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Mass Culturing of Mycetophagous Nematode Aphelenchus avenae (Nematoda: Aphelenchidae) in vitro System by Feeding on Pathogenic Fungus

Mass Culturing of Mycetophagous Nematode Aphelenchus avenae (Nematoda: Aphelenchidae) in vitro System by Feeding on Pathogenic Fungus

Salma Javed and Samreen Khan*

National Nematological Research Centre, University of Karachi, Karachi-75270, Pakistan.

 
*Correspondence | Samreen Khan, National Nematological Research Centre, University of Karachi, Karachi-75270, Pakistan; Email: samreenkhan3336@gmail.com 

Figure 1:

A and B. Isolation of Aspergillus niger from banana leaves; C. Pure culture of Fusarium oxysporum; D. Pure culture of Aspergillus niger; E and F. Interaction between fungus and nematodes; G. Fungal spores; H. Bunch of nematodes along with eggs; I. Mass culture of A. avenae after grew on F. oxysporum; J. Various embryogenetic stages of A. avenae. 

Figure 2:

Increasing in population of A. avenae on F. oxysporum with no growth on Aspergillus niger during three weeks incubation period.

Figure 3:

Exponential growth of A. avenae on F. oxysporum grew on PDA after three weeks incubation period. 

Figure 4:

A. Pure culture of F. oxysporum in test tube; B-C. Change in appearance and lessening of F. oxysporum after inoculation of A. avenae; D-E. Consuming fungus as well PDA medium by A. avenae.

Figure 5:

Culturing of A. avenae in a pot using brinjal plant.

Sarhad Journal of Agriculture

September

Vol.40, Iss. 3, Pages 680-1101

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