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New-HPLC-Method-for-Determination-of-Cefquinome

New-HPLC-Method-for-Determination-of-Cefquinome

Ijaz Ahmad1,5*, Haihong Hao1, Pascal Sanders2, Zafar Iqbal3, Saeed Ahmed4, Farhan Anwar Khan5, Zahir Shah5, Muhammad Ibrahim6 and Lingli Huang1

1National Reference Laboratory of Veterinary Drug Residues (HZAU) and MAO Key Laboratory for Detection of Veterinary Drug Residues, Huazhong Agriculture University Wuhan, China.
2Laboratory of Fougères, French Agency for Food, Environmental and Occupational Safety, Maisons-Alfort, France.
3Department of Agricultural Chemistry, The University of Agriculture, Peshawar, Pakistan
4Department of Biological Sciences, National University of Medical Sciences, Islamabad, Pakistan
5College of Veterinary Sciences, The University of Agriculture, Peshawar, Pakistan
6Animal Biotechnology Division, Institute of Biotechnology and Genetic Engineering, The University of Agriculture, Peshawar, Pakistan
 
Ijaz Ahmad and Haihong Hao contribute equally to this work.
 
* Corresponding author: drijaz@aup.edu.pk

ABSTRACT

The cefquinome samples from plasma were extracted and separated by HPLC on a reversed phase C-18 column with mobile phase of acetonitrile and formic acid (0.1%) solution- (90:10, v/v). The calibration curves were linear ranged from 0.01-5µg/mL. The limit of quantification (LOQ) and limit of detection (LOD) were 0.04 and 0.01 µg/mL. The recovery of cefquinome from plasma was 73.4%, 78.33%, and 77% for low, medium and high concentration samples. The intraday and inter day relative standard deviation were less than 15%. The Cefquinome were found to be stable for more than 3 month at -70oC (degree symbol) and more than 24 h at 4oC. The present method has been applied successfully for the pharmacokinetics study in cattle. After intramuscular administration, the mean peak serum Concentrations (Cmax) was found 2.95μg/mL and achieved after (Tmax) 1.50h. The elimination half-life (tl/2(el)) was 2.03±0.06h. The Mean Residence Time (MRT) was 2.58±0.05h and the area under curve from zero time to infinity (AUC0-∞) were 9.67±0.72μg/mL/h. The newly proposed method was shown to be simple, rapid, economical and sensitive compared to the previously reported method, and thereafter applicable for the pharmacokinetics study in animals.

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Pakistan Journal of Zoology

October

Pakistan J. Zool., Vol. 56, Iss. 5, pp. 2001-2500

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