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Anna-Maria Andersson*, Ann-Kristin Nyman, Per Wallgren

... hypergammaglobulinemia, plasmacytosis and immune complex disease. Due to lack of success in eradicating Aleutian disease (AD) by stamping out, a new strategy for disease control with focus on evaluation of disease progression has been proposed. For estimation of antibodies in serum and dried blood samples collected from mink, a VP2 ELISA (enzyme-linked immunosorbent assay) system was recently evaluated. However, such estimations of dis...

Paul I. Ankeli, Mashood A. Raji, Haruna M. Kazeem, Moses O. Odugbo, Nendir J. Umaru, Idowu O. Fagbamila, Livinus T. Ikpa, Obinna O. Nwankiti, Issa A. Muraina, Pam D. Luka and Nicholas D. Nwankpa

...isolate and identify Mycoplasma mycoides subspecies mycoides (Mmm) from the ear canal of apparently healthy cattle in Plateau State, Nigeria. One hundred and sixty six ear swab samples (n=166) were cultured from which eight (8) Mycoplasma species were isolated and characterized using conventional biochemical tests and polymerase chain reaction (PCR), respectively. Six (6) were observed to ferment glucose, reduce tetrazolium ...

Asma-ul-Husna1, Muhammad Sajjad Ansari2, Bushra Allah Rakha3, Rabea Ejaz1, Nemat Ullah1 and Shamim Akhter1*

...rm progressive motility, plasma membrane integrity, acrosomal integrity, viability and chromatin integrity was higher (P<0.05) in extender containing melatonin 0.1 mM and 0.5 mM compared to control. In conclusion, the addition of melatonin 0.1-0.5 mM to extender improved the post-thaw quality of buffalo bull semen.

...

Afzaal Ahmad Naseem1*, Maleeha Akram1, Sarwat Jahan2, Kiran Afshan2, Zubaria Iqbal1, Faheem Tahir3, Mazhar Qayyum1 and Syed Shakeel Raza Rizvi1

Shakeel Ahmed* and Sarwat Jahan

...After NMS administration plasma adipokines levels were determined in fed and fasting monkeys. Four intact adult male monkeys were used in this study. 50 nmol of NMS was injected intravenously. Blood samples were collected individually 60 min before and 120 min after NMS administration at 15 min intervals. The plasma adipokines concentrations were determined by using specific Enzyme Immunoassay (EIA) kits. 48 h fasting signif...

Tayiba Latif Gulraiz1, Arshad Javid1*, Syed Makhdoom Hussain2, Muhammad Shahbaz3, Irfan3 and Sharoon Daud4

...ngal (Cryptococcus, Histoplasma and Trichophoton) and six bacterial (Acaligenes, Azotobacter, Bartonella, Nitrsomonas, Pseudomonas and Salmonella) genera were isolated from guano while five fungal (Alternaria, Aspergillus, Chrysosporium, Exophilaand Scopulariopsis) and four bacterial (Bacillus, Corynebacterium, Listeria and Streptomycete) genera were common in bolus and guano samples. Seasonal variations were recorded in occurrence of various fungal and bacter...

Mostafa A. Abdel-Maksoud1,2, Fathy A. Abdel-Ghaffar2, Azza El-Amir2, Gamal Badr3 and Saleh Al-Quraishy1*

...nd granulocytes count in plasma samples of the live P. chabaudi infected group in comparison to either the control or the gamma irradiated P. chabaudi infected group. Additionally, live P. chabaudi infection has resulted in histopathological changes and increased apoptotic cell death in splenic tissue of infected mice. Moreover, infection with live P. chabaudi was accompanied with an increase in NO, H2O2, and MDA levels in splenic tissue of lupus mice in compa...

Rongrong Li1, Kun Li2, Xiaoqiang Wang2, Houqiang Luo2, Gang Qiu2, Hui zhang2, Yanfang Lan2 and Jiakui Li2,3*

Serkan Sayiner1* and Hilal Karagul2
...eifer blood selenium and plasma vitamin E levels were generally found within adequate ranges in all regions for both seasons. We suggest that feed supplements containing selenium should be added for the Iskele region during the winter months. For the Morphou region we advise that feed supplements should be added during summer due to low levels of selenium found in roughages.
...
Akbar Ali1,*, Khalil Mohamed2 and Fawzia Toulah3

 

...disease caused by Toxoplasma gondii parasite, is one of the most worldwide parasitic infections of warm-blooded animals including man. In most adults it does not cause serious illness, but it can cause serious health problems in infants, pregnant women and immune compromised patients. Abortion is one of the most serious outcomes in pregnant women. In this cross-sectional study, a total of 162 women of child bearing age were included from Rafha city of S...

Muhammad Asif Zahoor1, Zeeshan Nawaz1, Abu Baker Siddique1, Sajjad ur Rahman2 and Shahid Ali1*

...ext-align: justify;">Mycoplasma infections in gallinaceous birds are reported throughout the world and are caused by either Mycoplasma gallisepticum or Mycoplasma synoviae. Hence, in the present study we have detected Mycoplasma spp. from tissue samples collected from clinically sick (60 samples) and apparently healthy broiler birds (30 samples) in a com...
Hamayun Khan1,*, Momen Khan2, Muhammad Subhan Qureshi3, Shakoor Ahmad1, Ali Gohar4, Hameed Ullah5, FarmanUllah6, Arab Hussain7, Pershotam Khatri8, Said Sajjad Ali Shah1, Hamid Rehman1 and Azmatullah Khan1
... motility, viability and plasma membrane integrity. The data was statistically analyzed with one-way analysis of variance using SPSS. The highest significant (P<0.05) response was obtained in extenders containing 0.75% green tea extract for all studied parameters. Based on the findings of the current study, it can be concluded that supplementation of green tea extract in semen extender showed significant response on the post-thawed spermatozoa motility, via...
A. Hamid1,*, M. Ilyas2 and S. Kalsoom3
...d grains used locally on plasma cholesterol level of dietary-induced hypercholesterolemic rats. Insoluble dietary fiber (lignin and cellulose) was extracted from wheat and maize, while ß glucan was extracted from barley and sorghum. Male rats (n = 32), weighing 200-220 g were induced with hypercholesterolemia and were equally divided into 5 groups to study the effect of purified diet-AIN-93 G, wheat bran feed (WBF) , maize bran feed (MBF), sorghum ß...
Shabana1,*, Farah Ehsan1 and Shahida Hasnain1,2
...ith weight, BMI, fasting plasma glucose, total cholesterol level and LDLC had no effect on age, height, triglycerides, HDLC and leptin. In conclusion, Gly482Ser variant appears to be significantly associated with diabetes in Pakistani population. The variant appeared to exert its effect by changing serum fasting plasma glucose, total cholesterol and LDLC levels.
...
Habibun Nabi1, Saher Islam2, Amna Arshad Bajwa2, Imran Rashid1,*, Haroon Akbar1, Wasim Shehzad2, Kamran Ashraf1, Nisar Ahmad1 and Aneela Durrani3
...ccidian parasite, Toxoplasma gondii. One third of human population of the world is believed to be infected with T. gondii. Cats serve as final host of Toxoplasma gondii and are the main source of contamination of soil and water. Fecal samples from cats at Pet center of UVAS (Lahore, Pakistan) were screened for coccidian parasites through microscopy examination. DNA was extracted from positive feca...
Faiz Muhammad1,*, Syed Khurram Fareed1, Urooj Zafar1, Taseer Ahmed Khan2 and Aqeel Ahmad1
...pproach to detect Mycoplasma gallispeticum, Mycoplasma synoviae and nonpathogenic mycoplasmas at the same in a single PCR reaction collected from diseased chicken. Direct PCR from the clinical samples produced false negative results. Both culture and PCR were combined as culture-enhanced PCR approach. Three different DNA crude preparation methods were used and broth dilution ...
Rehana Shahida1, Tasnim Farasat1, Shagufta Naz1,* and Shahjahan2
...nic parameters including plasma glucose level, serum levels of insulin, IL-6, and TNF-α. Serum level of thrombomodulin was assessed as a measure of vascular damage. Different demographic parameters as age, BMI, waist /hip ratio, B.P., personal history and socioeconomic status were recorded. Fasting glucose and HbA1c was done by chemistry analyzer. Fasting serum insulin, IL-6, TNF-α and thrombomodulin levels were assessed by ELIZA. Gene expression w...
Ibrar Muhammad Khan1, Rifat Ullah Khan1,*, Muhammad Subhan Qureshi2, Tahir Usman2, Adnan Khan1, Zakir Ullah1 and Hamid Rehman1
...y, progressive motility, plasma membrane integrity, acrosomal and DNA integrity, seminal pH and dead sperm percentage in fresh and thawed semen. Data were subjected to one way analysis of variance. High sperm individual motility (85.12 ± 0.51%), progressive motility (20.25 ± 0.47%), plasma membrane integrity (82.25 ± 0.43%), acrosomal integrity (79.91 ± 0.51%), and DNA integrity (98.54 ± 0....
Abdul Malik1,2, Ghulam Abbas1,*, Abdul Ghaffar3, Sara Ferrando4 and Lorenzo Gallus
...atocrit, cholesterol and plasma triglycerides were similar among fish fed on different salinity level (P < 0.05).
...
Saara Ahmad1,*, Iftikhar Ahmed2, Saida Haider3, Zehra Batool3, Fatima Ahmed4, Saiqa Tabassum3,6, Syeda Madiha3, Tahira Perveen3 and Saad Bilal Ahmed5
...six weeks. The levels of plasma cholesterol, progestril, estradiol, and androgens were estimated at the end of the experiment. The ovaries of the rats were collected, weighed and histopathologically evaluated for the development of cysts. It was seen that the ovaries of the group treated with caged chicken meat showed increased cholesterol levels, imbalanced steroidal sex hormone levels, increased ovary weight and development of the cysts upon histopathologica...
Alaeldein M. Abudabos*, Abdullah N. Al-Owaimer, Elsayed O.S. Hussein and Mutahar H. Ali
...umulative performance or plasma mineral status.
...
Nasir Abbas1, Muhammad Suleman1, Nazir Ahmad Khan2,*, Ijaz Ali3, Mubashir Rauf1,5 and Sadeeq ur Rahman4,*
...nd involvement of Mycoplasma gallisepticum (MG) in cases of chronic respiratory disease (CRD) in poultry and pheasantry birds of Northern Pakistan is underreported. In this study we report on the seroprevalence and isolation of MG from a total of 2341 poultry and 24 pheasantry birds. Overall, seropositivity of 46.56% (1090/2341) and 27.2% (6/24) against MG was observed among poultry- and pheasantry- birds, respectively. Broiler breeder and broiler showe...
Nilgün Paksoy1, Hikmet Dinç2 and Serap Kılıç Altun3,*
...d by inductively coupled plasma - mass spectrometry (ICP-MS). Minimum and maximum levels of Na, Mg, P, K, Ca, Fe, Cu, Zn in donkey milk samples were 108.6-202.7, 48.6-76, 355-670, 565-1053, 279-637, 1.86-5.8, 0.052-0.277, 1.78-10.97 mg L-1; in goat milk samples were 201.7-1087, 81.7-138.8, 818-1132, 972-1802, 678-1323, 1.91-4.48, 0.052-0.576, 1.72-4.68 mg L-1; in sheep milk samples were 219-640.5, 119.7-238.9, 946-1895, 881-1474, 1103-196...
Chuanpeng Zhou1, Heizhao Lin2,*, Zhong Huang2, Jun Wang1, Yun Wang1 and Wei Yu2
... glucose group has lower plasma glucagon content 12 h after oral administration, higher muscle and hepatic glycogen contents 6 h after oral administration (P<0.05). The hepatic pyruvate kinase activity peaked 6 h after oral administration in both doses of glucose groups and thereafter started to decrease 24 h after oral administration. While the hepatic PEPCK and G6Pase activities decreased 9 h after oral administration in both doses of glucose groups and t...
Mohammed Al Bratty1, Hassan A. Alhazmi1, Somaya Jadoh Ogdi1, Jana Ahmad Otaif1, Abdul Jabbar Al-Rajab2, Mohammad Firoz Alam3 andSadique Akhtar Javed1,*
S. Jalbani1, N. T. Narejo2,*, P. Khan2 and Y.M. Jalbani3
... may be due to different plasma viscosity and specific gravity of the erythrocyte is responsible for different ESR values.
...
Hanif Ur Rahman1, Umer Saddique1, Zahoor-ul-Hassan1, Shakoor Ahmad1, Muhammad Kamal Shah1, Said Sajjad Ali Shah1, Farhan Anwar Khan1Fazli Rabbani2, Muhammad Asif Hussain2, Attaur-Rahman2, Irshad Ahmad3,4 and Sadeeq ur Rahman2,*
...ase, is caused by Mycoplasma mycoides (MM) - a cluster of six member species. However, literature is not available regarding the causative agents of CCPP in sheep and goats in Northern Pakistan. The current study was thus aimed to identify relative abundance of members of MM in small ruminants suspected of CCPP in Northern region of Pakistan. A total of 300 samples, (150 sheep and 150 goats) were randomly collected from nasal discharge, pleural fluid an...

Bata Shalangwa Ishaku1*, Maimadu Abdullahi1, Dakwang Nalong1, Rengkat Jonah1 and Olabode Mayowa

...ne the prevalence of Toxoplasma gondii antibodies in pigs, sheep and goats at slaughter in Jos, Plateau State Nigeria. Five hundred (500) serum samples comprising of 300 pigs, 100 each of sheep and goats were collected and analyzed for Toxoplasma gondii antibodies (IgG) using latex agglutination test (LAT). Serum samples with LAT titer >10 IU/ml were considered positive. The study showed that 176 of the 500 samples analyz...

Mushtaq Hussain Lashari1*, Fozia Afzal1 and Umer Farooq

...urally infected with Toxoplasma gondii (T. gondii). Blood samples of chicken (n=71), were collected from Cholistan areas of Bahawalpur (Punjab), Pakistan. Serum samples were tested for toxoplasmosis with a commercial latex agglutination kit (Antec Diagnostic products, UK). The overall prevalence was 26.76%. The significant (P<0.05) higher seroprevalence was observed in males (34.28%) than in females (19.44%). For analysis of hematological parameters blood s...
İnan Kaya1,*, Muhitdin Yılmaz2, Müge Mavioğlu Kaya3,Abdulsamed Kükürt4 and Mahmut Karapehlivan5
...ve stress index (OSI) in plasma were evaluated as well as AST and ALT levels in the liver. Plasma OSI and liver AST levels in the groups with carbaryl and 2,4-D were compared with the control group, and they were found to be significantly higher. As a result, it was concluded that carbaryl and 2,4-D administered under of LC50 value caused liver damage by increasing the oxidative stress of Capoeta capoeta.
Asima Bano1, Hafiz Muhammad Tahir2, Hira Sherawat3, Muhammad Mutlib4, Muhammad Arshad5, Muhammad Akram Qazi6, Sajida Naseem5, Rabia Ishaq1, Iram Liaqat2 
...r. For enzyme estimation plasma samples from the patients suffering from breast cancer and age matched healthy individuals (control) were collected. The results revealed decline in the level of these enzymes in breast cancer patients as compared to healthy individuals because of this decline the overall level of protein was also found to be decline. It is concluded from the study that these enzymes can be used as biomarker in the diagnosis of breast cancer.&nb...
Tünay Kontaş Aşkar1,*, Şinasi Aşkar1, Olga Büyükleblebici2 and Murat Güzel3
...ificantly high levels of plasma malondialdehyde, nitric oxide, myeloperoxidase, adenosine deaminase and cytokines and significantly low levels in total antioxidant capacity and glutathione levels were found in dogs with leishmaniasis when compared with the healthy controls. According to the result of this study, oxidative stress and inflammatory changes occur in CVL. And this is the first report for myeloperoxidase (MPO) activity in CVL. Therefore investigatio...

 Asma Karim1, Wajid Ali1*, Noreen Ahmad1, Muhammad Irfan2, Hafiz Abdullah Shakir3

Histological and biochemical study of liver of silver carp (Hypophthalmichthys molitrix) after acute exposure to pyrethoride (deltamethrin)
...mes AST and ALT in blood plasma of exposed fish as compared to control fish.
These results suggested that deltamethrin is highly toxic for aquatic organism like silver carp causing malfunctioning
of liver.

...

 Khurram Shahzad1*, Muhammad Naeem Khan1, Farhat Jabeen2, Nasreen Kosour3, Muhammad Sohail1, Muhammad Khalil Ahmad Khan1, Munir Ahmad1

Bioaccumulation of manufactured titanium dioxide
A.M. Abdul Azeem, A.N. El Shahat* and Ashraf M. Mounir
...r lipid peroxidation and plasma levels of caspase 3 associated with significant increase in the levels of luteinizing hormone, follicle stimulating hormone and testosterone in addition to marked improvement in the level of total antioxidant capacity and glutathione content and the activity of superoxide dismutase activity and catalase activity of the liver and testes of lead-intoxicated rats. In conclusion GPE could ameliorate the damage and apoptotic effects ...
Muhammad Saqib Shahzad1, Amina Arif1,*,Saeeda Kalsoom2, Javed Iqbal3, Shahnam Shafique2, Muhammad Shahid Nadeem4 and Rafique Ahmed5
...ge and viral load in the plasma. The plasma values of ALT have shown no specific relation with SVR and NSVR. Genotype 3a was the most prevalent (69.7%) followed by 3b (13.6%), mixed genotypes (10.5%), 1b (3.2%), 1a (3.1%) among the investigated patients. We found highest SVR against genotype 3a (79.4%), followed by 3b (56%), 1a (33%), 1b (37.3%), 3a+3b mixed infection (29%), and 1a+1b mixed infection 24.9%. In summary, we fo...
Tamoor Azeem1,*, M. Yasin Tipu1, Asim Aslam1, Sajjad Ahmed2, Salman Ahmed Abid1, Abdullah Iqbal3, Naeem Akhtar4, Muhammad Saleem4, Aamerzish Mushtaq4 and Sajid Umar4
...i>Babesia, and Anaplasma. The current study was executed to investigate haematobiochemical alterations in camels during haemoparasitism. Blood samples were collected from 200 camels of either sex and different ages from district Attock. Samples were processed for haematobiochemical analysis and thin smear stained with Field stain according to standard procedure. Out of 200 camels 46% of them have blood parasites. Trypanosoma was the most prev...
Samiah Shahid1,Jawaria Shaheen1, Wajeehah Shahid2, M. Waheed Akhtar1,3 and Saima Sadaf1,*
...n in patient and control plasma samples of ALL. Three highly reported reference genes namely RNU6-2, mir-16-5p and cel-mir-39-1 were selected as the candidate genes for normalization. Preliminary, quantification was performed through real time PCR in eight samples. The geNorm algorithm and comparative delta Cq method were used for selection of suitable reference gene out of the three candidate genes. The validation studies were, thereafter, performed on...
Tafail Akbar Mughal1, Muhammad Zubair Saleem2, Shaukat Ali3,*, Khawaja Khurshid Anwar1, Muhammad Majid Bashir1, Muhammad Babar4 and Muhammad Adeeb Khan1
... resulted in increase in plasma ALAT and decrease in LDH. Vitamin E pre-treatment abolished CCl4-induced changes in the activities of these enzymes. Blood glucose content was increased while cholesterol content was decreased. Vitamin E pre-treatment abolished only CCl4-induced change in blood glucose content but failed to abolish CCl4-induced change in cholesterol content. Glucose, urea, lipids, cholesterol contents in liver we...
Shehzad Ghayyur1,3, Sadia Tabassum1, Munawar Saleem Ahmad2Naveed Akhtar1 and Muhammad Fiaz Khan1,*
...essor agent, while total plasma proteins and triglyceride level were significantly decreased (p < 0.05).

...

 Jawaria Shaheen1, Samiah Shahid1, Sabahat Shahzadi2, M. Waheed Akhtar1,3 and Saima Sadaf1,*

...0b) has been seen in the plasma samples of TNBC patient´s samples relative to non-TNBC control samples. The scatter plots and other statistical analysis have given differences in expression of four miRNA panel with significant p-value (p< 0.001), respectively. Kruskal Wallis test and Mann Whitney U test have clearly showed significance and association of miRNA panel with clinicopathological characteristics of breast cancer which have revealed their ro...
Junli Sun1,2,3, Lin Bai1 2, Xiaogan Yang1,2, Yangqing Lu1,2, Shengsheng Lu1,2,* and Kehuan Lu1,2,*
...puncture of egg zona and plasma membrane had the highest cleavage rate and blastocyst rate of porcine parthenogenetic embryos, which was significantly higher than Group I with intact oocytes (P<0.05) and Group III with the puncture of zona and plasma membrane and aspiration egg cytoplasm (P<0.05) on the cleavage rate, and was extremely significantly higher than Group III on the blastocyst rate (P<0.001). Raman spect...
Shujjah Haider1,*, Ayesha Maqbool1, Tariq Pervez1, Saima Parveen1, Arfan Ahmad2, Zahid Iqbal3, Javed Iqbal3, Shahid Mehmood3, Amanullah Khan4 and Sajid Umar5
...asmosis caused by Mycoplasma gallisepticum (MG). The distribution pattern of MG in layer flocks is not known in Chakwal. Keeping this in mind, current study was designed to know about distribution of MG. The present study was conducted on 25 commercial layer flocks from different regions of district Chakwal, Pakistan. A total of 358 blood samples were collected from different regions of district Chakwal and subjected to serological tests including enzym...
 

Aftab Shaukat1,*, Khalid Medmood3, Irfan Shaukat2, Tauseef ur Rehman3, Muhammad Ahsan Naeem1,5, Ashar Mehfooz1, Muhammad Ijaz Saleem1, Zia-ud-Din Sindhu1, Shahid Ali Rajput4, Mubashar Hassan1,4, Saqib Umar1, Muhammad Ali Jamil1, Rao Zahid Abbas1 and Anas Sarwar Qureshi1

 

...propionate against Anaplasma marginale (A. marginale). For this study, the blood samples of three hundred and sixty nine cattle were collected. Giemsa stain of blood smears showed 10.84% infection of cattle with Anaplasma. A. marginale positive cases were further confirmed through PCR. Among risk factors, age (χ2=19.35, P value=0.001), breed (χ2=29.08, P value=0.000), freque...
Abd El-Nasser Ahmed Mohammed1,2,*
...Cs, hematocrit, WBCs and plasma total protein values in addition to significant hypoglycemia compared to control. The quality of oocytes and reproductive performances were improved due to N. sativa oil supplementation. N. sativa oil rescued the depression in oocytes number and quality after ovarian transplantation. N. sativa oil could not improve hypothermia and hyperglycemia due to general anesthesia. In conclusion, supplementation of ...
Sabbah M. Allam, T.M. El-Bedawy, M.H. Bakr and A.E.M. Mahmoud*
...level of antioxidants in plasma, and presence and count of fecal pathogenic bacteria e.g. E. coli and Salmonella spp. Forty lactating Holstein cows, weighing 550±50 kg, with an average of 20 kg daily milk yield, were randomly divided into four groups (ten in each group). Animals were fed rations containing DOP at 0, 25, 50 and 75% substitution of yellow corn grains (R1, R2, R3 and R4, respectively). Digestion trail was conducted in ...
Lin Sun1, Xiaofang Bu2, Jian Wang1*, Xiaorui Liu1 and Zhanyi Kong2
...logical injury of Mycoplasma pneumoniae pneumonia (MPP). However, the exact mechanism of CXCL8 and its mRNA in the children with Mycoplasmal pneumonia needs to be further clarified. The concentration of the CXCL8 in serum and the level of CXCL8 mRNA in PBMCs of forty-eight children were dynamically measured by ELISA and PCR. The ratio of lgcDNA/lgGAPDH was regarded as the extreme level of CXCL8 mRNA. The serum level o...

Olufisayo Bamidele* and Safiriyu Idowu Ola 

...t where appropriate. The plasma LH for the AI groups peaked at 60mins while it peaked at 120mins for the control. Kindling rates were 10%, 10%, 60% and 50% respectively for the groups. There was no significant difference (p>0.05, t-test) between the litter size and total live kits obtained for 10µg and 15µg groups. Kit weight at birth showed no statistical difference (p>0.05, ANOVA) among all the groups. There was no still born in the control...
Chaman Ara1*, Asmatullah1, Sehrish Kanwa1, Asma Chaudhary2, Ayesha Siddiqua1
...in was observed in blood plasma. Urea level showed a remarkable decrease, whereas creatinine level increased but not significantly. Histological examinations exhibited pyknosis, necrosis, vacuolations, increased sinusoidal spaces, karyomegaly, glomerulosclerosis, glomerulonephritis, epithelium degeneration, spermatocytes exfoliation, tubular degeneration and aspermia in liver, kidney and testis respectively. Levofloxacin causes deleterious effects on liver, ki...
Ahsan Anjum1, Asim Aslam1, Raheela Akhtar1, Tahir Yaqub2,  Muti-ur-Rehman Khan1, Rizwana Sultan3, Saba Usman1Aneela Zameer Durrani4 and Muhammad Usman4*
...ges and caused by Mycoplasma mycoides subsp. mycoides. This study was carried out in abattoirs of Lahore, Kasur and Jhang districts of Punjab, Pakistan. Different tissue samples were collected from 560 cattle showing respiratory signs at slaughter. Lung samples were used for detection of CBPP through PCR targeting 16S ribosomal RNA. Furthermore, lungs, liver, kidney and lymph node were collected for histopathological examination. Of the 560 sampl...
Saara Ahmad1,*, Iftikhar Ahmed2, Saida Haider3, Zehra Batool4, Laraib Liaquat3, Fatima Ahmed5, Asra Khan1, Tahira Perveen3, Mirza Jawad ul Hasnain6, Saima Khaliq7 and Saad Bilal Ahmed8
...he liver, and had raised plasma TNF-α and AFP-levels. This study shows that damage to liver cells is caused by consumption of commercial feed for chickens and their meat which is considered safe for human consumption.
...
Elmas Ulutaş1,*, Abdullah Eryavuz2, Aziz Bülbül2, Abdur Rahman3,*, İsmail Küçükkurt4 and Cangir Uyarlar5
...on increased (p<0.05) plasma urea nitrogen and glutathione but decreased (p<0.05) leptin and malondialdehyde concentration while other parameters remained unaffected. No difference was observed in ruminal pH between the treatment groups. Ruminal ammonia and number of protozoa were decreased (p<0.05) with 700 ppm and 1000 ppm zinc supplementation. Rumen Zn concentration increased (p<0.05) in the goats fed with 1000 ppm zinc, whereas there was no dif...
Mubashra Salim1, Omeira Ibrahim1, Hugo Vilhena2,3,4, Carla Maia5, André Pereira5, Maria Shahzeen1, Shabana Kalsoom1, Asim K. Mahmood6 and Furhan Iqbal1*
...mall;">Hemotropic mycoplasmas (hemoplasmas) are obligate Gram-negative bacteria that target red blood cells, and infect a wide range of hosts including cats, dogs, domestic ruminants, pigs, rodents and humans. The present study was designed for the molecular detection of Mycoplasma haemofelis and Candidatus Mycoplasma haemominutum in...
Ahmet Ozer Sehirli1,2, Serkan Sayiner3*, Ayliz Velioglu-Ogunc4, Nedime Serakinci5, Emel Eksioglu-Demiralp6, Berrak Yegen7, Feriha Ercan8 andGoksel Sener2
... cells was analysed from plasma samples. Tissue samples from the brain, heart, aorta and kidneys were used for analysis of the collagen content besides tissue luminol, lucigenin, malondialdehyde (MDA) and glutathione (GSH) levels. A significant difference was determined between the CRF group and the control group with regard to heart rate, blood pressure, serum creatinine, BUN, LDH, cytokines and urinary electrolyte levels. Furthermore, monocyte and neutrophil...

Amit Sharma1* and Pankaj Sood2 

...l variation of the sperm plasma membrane. Different factors affect affects the semen cryopreservation viz. species, breed, semen collection technique, collection season, extender composition, type of cryoprotectant used, cooling rate, equilibration time, freezing and thawing rate. All these factors should be given due credit for achieving better post-thaw post thaw quality of semen to be utilized in different advanced reproductive techniques. 

...
Hifzulrahman1, Muhammad Abdullah1*, Muhammad Uzair Akhtar2, Jalees Ahmed Bhatti3, Muhammad Saadullah1 and Muhammad Naveed-ul-Haque2
...lk production, milk fat, plasma metabolites, and reproductive parameters in early-lactating Nili Ravi buffaloes. Sixteen early-lactating Nili Ravi buffaloes received 2 treatments in a completely randomized design with a period length of 120-d. The treatments were 1) Ctrl = basal diet without supplementation of fat, 2) Ca-FA = basal diet with calcium salts of palm fatty acids supplementation. The Ca-FA treatment increased the 3.5% fat-corrected milk yield by 11...
Mian Adnan Kakakhel1, Faryal Gohar1, Zahid Anwar2, Raza Ullah1, Muhammad Attaullah3, Shahbaz Ahmad4, Aamir Khan5, Kalimullah5*

Markus I. Francis1*, Paul I. Ankeli2, Clara N. Kwanashie1, Jibril Adamu1, Lushaikyaa Allam1, Mashood A. Raji3, Godwin O. Egwu4, Flavio Sacchini5 and Massimo Scacchia5

Detection of Mycoplasma bovis from Cattle Presented for Slaughter in Adamawa and Taraba States, Northeastern Nigeria
...ext-align: justify;">Mycoplasma bovis is the second most important mycoplasmas causing a variety of diseases in cattle worldwide. This study was aimed to report the PCR identification of M. bovis from slaughtered cattle in northeastern Nigeria. Four hundred and eighty (480) samples of lung tissues (180), nasal swab (180), ear swab (60) and pleural fluid (60) were collected from 190 heads of cattle at slaughter in Yola and Ja...
Maryam Yousaf1, Naveed Shahzad2, Zeeshan Mutahir1 and Moazzam Ali1*
...MRP-1 trafficking to the plasma membrane by Rab21 is a potential strategy to overcome multi drug resistance in cancer cells.
...
Muhammad Rizwan1*, Bilal Atta1, Ana Maria Marino de Remes Lenicov2, Roxana Mariani2, Arshed Makhdoom Sabir1, Muhammad Tahir3, Misbah Rizwan4, Muhammad Sabar1, Ch. Muhammad Rafique1, Muhammad Afzal5
...iids are vectors of phytoplasmas, mycoplasmas and prokaryotes-like associated to the class Mollicutes. Specimens of planthoppers were collected from the rice fields and surrounding weeds. A list of Fulgoromorpha is provided, with distributional and biological records as well. Records are extracted primarily from field data and specialized reference sources. Seven species from two different families of Fulgoromorpha were rela...

Ummad ud Din Umar1, Syed Atif Hasan Naqvi1*, Ishfaq Ahmed1, Ateeq ur Rehman1, Muhammad Asif Zulfiqar2, Sibghat Ullah3, Shah Pasand4, Zehri Khan3 and Abdur Rehman5

Prevalence and Serological Detection of Spiroplasma citri, a Cause of Citrus Stubborn Disease in Southern Punjab, Pakistan
...xt-align: justify;">Spiroplasma citri, a fastidious pathogen, is considered as the main cause of Citrus little leaf disease (Citrus stubborn disease) which is not only responsible for the low citrus production in terms of quality and quantity but also moving the citrus industry to down fall in citrus growing areas throughout the world. Current research was organized to study the distribution of disease using the serological based detection methods by conductin...
Bushra Ismail Khan1, Shamim Akhter1, Sanwal Aslam2* and Rabea Ejaz1
...ssed for sperm motility, plasma membrane integrity (PMI), dead sperm percentage and the live sperm percentage. The data on the role of PVP on different parameters of semen quality were analyzed by using ANOVA and RCBD. Higher percentage (P< 0.05) of sperm motility (66.1±7.51 and 59.4±10.72) and PMI (72.9±5.39 and 75.7±6.5) was observed in extenders having 0.05 mM and 0.1mM PVP compared to extenders having 1.5mM PVP and control. T...
Rabea Ejaz1*, Saima Qadeer2,Muhammad Sajjad Ansari3, Bushra Allah Rakha4Shazia Shamas5, Iram Maqsood1, Asma Ul Husna6, Asima Azam1 and Shamim Akhter6
...m motility, liveability, plasmalemma integrity and viability were increased in extender (P<0.05) having 20.0 ng/mL of arachidic acid compared to 25.0 ng/mL, 30.0 ng/mL and control. However, sperm chromatin integrity was equally improved in experimental extenders having arachidic acid compared to control. Sperm abnormalities were reduced in experimental extender with 20 ng/mL of arachidic acid compared to other experimental extenders containing arachidic aci...
Shakeel Ahmed* and Sarwat Jahan
...after NMS administration plasma testosterone (T) and growth hormone (GH) levels were determined in four normally fed and 48 hours fasted adult male rhesus monkeys.Fifty nmol (50 nmol)of NMS was injected through a cannula affixed in saphenous vein. Blood samples were collected individually 60 minutes before and 120 minutes after NMS administration at 15 minutes intervals. Plasma T and GH concentrations were determined by usin...

Faiqah Ramzan1,2,*, Irfan Zia Qureshi2 and Muhammad Haris Ramzan3

...bserved in the levels of plasma FSH. The concentrations of testosterone and LH were found to be significantly reduced in kisspeptin treated groups of 1µg dose (p<0.01) and 1ηg dose (p<0.05) whereas the dose of 10ρg had shown no significant change. Kisspeptin doses of 1ηg and 1µg lead to significant reduction in quantity of elongated spermatids and daily sperm production (p<0.05; p<0.001), step 7 spermatids (p<0.05; p<0...
Mushtaq Ahamd Khan1, Ziaul Islam2, *, Amin Ullah Jan3, Kamran Khan2 and Abdullah Shah3
...disease caused by Toxoplasma gondii that infects a wide range of warm-blooded animals including humans. Congenital infection with T. gondii during pregnancy can result in severe abnormalities in infants such as hydrocephalus and mental retardation. The present study was conducted to estimate seroprevalence and potential risk factors in acquiring T. gondii infection by child-bearing age women in Dir Khyber Pakhtunkhawa, Pakistan. A cross se...
Mumtazur Rahman1, Farhan Anwar Khan1,*,Umar Sadique1, Ijaz Ahmad1, Shakoor Ahmad1, Faisal Ahmad1,2, Hayatullah Khan1,3, Muhammad Saeed1, Faiz Ur Rehman1, Ibrar Hussain1, M. Faraz Khan1, M. Izhar ul Haque1 and Hanif-ur-Rehman1,3

... (CCPP) caused by Mycoplasma capricolum subsp. capripneumoniae (Mccp) is a highly contagious and deadly disease of small ruminants, especially goats. It is an economically important disease, causing high morbidity and mortality in goats. More recently Mccp Pakistan strain has been identified and isolated by our laboratory. Alarmingly, antimicrobials in animals are inappropriately using and resultantly leading to antimicrobial resistance (AMR) inc...
Saima Yaqub1, Tahir Yaqub1*, Muhammad Zubair Shabbir2, Asif Nadeem3Aziz-Ul-Rahman1, Muhammad Furqan Shahid1, Zarfishan Tahir4 and Nadia Mukhtar4
...Pakistan. A total of 201 plasma samples were collected from IDUs. Samples were first screened using HIV-1 Ag/Ab Combo test and then antigen positive samples were amplified for HIV-1 pol gene (1084 bp) and analyzed. Initial screening showed a total of 49 HIV samples positive (24.37%; 95% CI: 18.96-30.76). A substantial association of HIV incidence was observed in individuals with HCV infection (36.84%; 95% CI: 28.55-45.99; p<0.0001) followed by indivi...
Adnan Khan1, Rifat Ullah Khan1, Muhammad Subhan Qureshi2Muhammad Mobashar3, Ali Gohar4, Shakoor Ahmad1, Kamran Khan5Hamayun Khan1 and Shabana Naz6*

 

...gressive motility, sperm plasma membrane integrity were observed in T2. It reflects high damages at T3 and T1 due to cryo shock and oxidative stress respectively. Sperm DNA integrity and pH were increased linearly from T1 to T3. The dead sperm percentage was significantly (P<0.01) low in groups with semen sample treated with T2 compared to other groups. Significantly (P<0.01) high sperm individual motility percentage was observed for T2 than T3 and T1. S...
Rabia Riaz1, Khushi Muhammad1*, Masood Rabbani1, Muhammad Amjad Iqbal2, Aamir Riaz Khan2, Sadia Sarfaraz1, Mudassar Naseer3 and Khalid Majeed1
...ional GDP (1.3%). Mycoplasma gallisepticum (MG) causes avian mycoplasmosis and is only controlled through mass vaccination. Antibody response of birds to inactivated MG vaccine is poor. On 7th day of age (broilers: n=40) each bird of group A, B, C and D (n=8) was vaccinated (subcutaneous: 0.3ml) with G-MG, O-MG, G-MG+NDV (New castle Disease Virus) and O-MG vaccines, respectively. Serum samples were collected on 21, 28 and 35 days post vaccination for an...
Muhammad Younis1, Muhammad Irfan-ur-Rehman Khan1*, Mustansar Abbas1Ali Murtaza1, Imran Mohsin2, Muhammad Shahzad3 and Muhammad Zahid Tahir1
Yuanyuan Zhang1*, Liping Song1, Hui Guo2, Jun Wu1, Xiaoli Wang1 and Fangbin Yao1
...he model experiment. The plasma and liver were collected for the test after 72 h. Results showed that there was a significant (P<0.05) increase in weight gain rate (WG) and a special growth rate (SGR) of fish fed feeds supplemented with 60 and 120 mg curcumin kg-1. When fish were induced by CCl4 after 72 h, fish fed the diet supplemented with 120 mg (P5) curcumin kg-1 had significantly (P<0.05) lower
Jiawei Hong1,2, Mingqiang Chen 1,2, Zhenghua Deng1,2, Youning Li1,2 and Yu Wang1,2*
...tive abundance of Mycoplasma, dropped down from 58.38% to 3.85%, and Vibrio increased (from 15.23% to 40.8%) to become the dominant genus. The hierarchical clustering heat map analysis and principal component analysis (PCA) showed that the microbial community of the high dosage group (10 mg/L) was clearly different from the other two groups. Overall, enrofloxacin at high dosage of 10 mg/L significantly altered the community diversity of noble sca...

Mokbel, Samah A.1,Ahmed K. El Attar1; Azza G.Farag2.

...pt. The
Phytoplasma associated witches‟-Broom has been detected on symptomatic hibiscus
plants. Samples of hibiscusleaves were collected from El Giza, Alexandria, Qlubia,El-
Fayom, and El-Mansouragovernoratesand analyzed for phytoplasma infection.The
collected hibiscus plants showed characteristic symptoms for Phytoplasma associated
...
M. A. S. El-kady1; A. B. Badr2; Ahmed K. El-Attar1, Hoda M. A. Waziri1 and Kh. E. A. Saker1
...div>...
Eman A. Ahmed1, Osama Y. Shalaby2, Emad F. Dwidar2, Samah A. Mokbel1, Ahmed K. El-Attar1 
...and distribution of phytoplasma associated with diseased
tomato plants (Solanum lycopersycum L.), and to identify and classify the phytoplasma
involved. A detection of infected tomato plants, which showed symptoms of big bud,
witches'-broom and phyllody, in all regions of the screened governorates, reacted
positively when assayed by nested polymerase chain reactions (PCR) using...

Sadiq Haladu1*, Taiwo Oladoye Akande2 and Mudassir Nasir3

...profiles. The results of plasma lipid profile showed that diet 4 (78.76 mg/dl) is significantly low in HDL compared to other dietary treatments. Egg total cholesterol showed significant reduction in diet 5 (254.65 mg/dl) observed when compared to the control diet (314.56 mg/dl). The result of serum analysis also showed significant difference in all the tested parameters. It was concluded that supplementation of layer diets with 2% fluted pumpkin and moringa im...

Manar F. Seioudy1, Magda M. Sayed1, Ahmed A. El-Sanousi2 and M. A. Shalaby2

...PCR and detection of mycoplasma as possible bacterial contaminant using PCR technique. All of the four batches were positive when tested using specific primers and they were free from BVDV or mycoplasma contamination. Results in this study showed that molecular techniques could be used for rapid evaluation of PPR vaccine including RT-PCR for identity testing and for detection of BVDV as adventitious contaminant and PCR assay...
Eman A. Ahmed1, Osama Y. shalaby2, Emad F. Dwidar2, Samah A. Mokbel1 and Ahmed K. El-Attar1
...nfected tomato with phytoplasma with healthy ones in order to reveal anatomical modifications caused by the infection process. The results obtained showed that infection of tomato plants with phytoplasma led to an increase in stem diameter by 10.23% as well as greatly increase in measurements of the other stem components while the diameter of pith was decreased by 38.46%. This infection was led to an increase in the diameter...

Salama M. El-Saghir1, 2

...spected symptoms of phytoplasma infection were detected. These involved leaf yellowing and
withering, reduction in fruit size, malformation of fruits (where two or three fruits were attached
together), and tree death were observed.
Objective: The objective of this study is to characterize the nature of this disease complex through
examining the incidence of PNRSV and phytoplasma
Rasha M. Mahrous1,4, Ahmed K. El-Attar2, Ahlam A. AlWatban1, Sohair I. EL-Afifi3,
Nagwa M. Aref1,3
...iv>...
Sahar Mubashar*, Tariq Mukhtar and Nasir Ahmad Khan
...cacy. In Pakistan, blood plasma therapy is in high demand but involves the risk of transmission of blood borne pathogens. As Pakistan lacks medical facilities. the government has to get testing kits and primers from other countries. Only few quarantine centers are available and there is shortage of drugs, beds, trained doctors and paramedical staff. Unfortunately, no vaccine is available yet so the only management strategy is the prevention of infection by wea...
Saira Gul1,2, Sohail Ahmed2*, Tahir Usman1*, Khalid Khan3, Sultan Ayaz1, Saleha Gul4 and Nawab Ali5
...sial parasite Anaplasma marginale. This study investigated the prevalence of A. marginale in cattle on different commercial farms in subtropical conditions of Khyber Pakhtunkhwa (KP). A total of 120 cattle from different farms were randomly sampled and tested for the presence of A. marginale between May to August 2016. The study revealed that 15.0%, 20.8% and 29.1% of sampled animals were found positive f...

Shimaa M. Gad1, Ahmed A. Kheder1, Mohamed A. Awad 2

...iv>...
Aamer Abbas1, Jabbar Khan1*, Mir Abid Hassan2, Asif Qayyum3 and Hamed Shafiq4
...sperm samples from semen plasma, sperm counting and DNA extraction for assessment of clinical and molecular attributes was done using standard protocols. Healthy women were used as negative controls. This study revealed significant concentrations of Zn, Cd, Cr, Pb, Cu. Except Zn and Pb, the other 3 metals were found below the permissible limit set by WHO. Interestingly, the infertile males having maximum age limit of above 47 years and highest marriage ages du...
Wenjuan Yan1, Qunlan Zhou2, Bo Liu2,3*, Cunxin Sun2, Huimin Zhang3 and Changyou Song2
...>significantly increased plasma ALT activity, urea nitrogen, total protein, albumin and globulin contents, while significantly decreased AST activity in 400 mg Kg-1 group. Haemolymph SOD and GSH-PX activity was enhanced significantly in 400 and 800 mg Kg-1 group, respectively, while MDA content was significantly decrease in these two groups. I. cicadae did not affect the muscle cooking loss, but significantly increased the compress...
HuaBin Zhang1,2, YaSen Li1, Tao Pan1, Peng Yan1, En Li1, Hui Xue1 and XiaoBing Wu1,*
...nal changes of leptin in plasma of Chinese alligator by radioimmunoassay method. Our results demonstrated circulating leptin levels varied significantly over the season. Therefore, we hypothesize that leptin is involved in nutrient stores, absorption and utilization, and reproductive behavior of the Chinese alligator.
...
Rizwana Sultan1, Asim Aslam1, Muhammad Yasin Tipu1, Habib ur Rehman2, Saba Usman1, Ahsan Anjum1,*, Muhammad Saeed Imran1, Muhammad Usman3 and Muhammad Zahid Iqbal3
...lds of caeca, mild lymphoplasmacytic infiltration in the periportal area of the liver and mild depletion of lymphocyte in the bursa of Fabricius. The seven clades of avian Eimeria species strongly support that E. necatrix and E. tenella were closely associated and placed in the same sister clade with high bootstrap support (98%). Our two isolates RSI and RSII showed a homology index of 99.82% (nucleotide level) and 99.47% (amino aci...
Ou-Mei Hao1*, Xue-Feng Wang1, Zhi-Jun Yue1, Chun-Hong Nan1,Yan Yu1,Tong Zhang2,Yu-Feng Liu3 and Xue Zhao1
...nflammation after Mycoplasma pneumoniae pneumonia (MPP) infection and compare the therapeutic effects of Qing Zhi Zhi Ke formula (QZZKF), QZZKF combined with azithromycin (AZM) and AZM on experimental mice. Fifty female BALB/c mice were randomly divided into five groups: control, model, AZM (0.01 g/kg/day), QZZKF (10.16 g/kg/day), AZM (0.01 g/kg/day) combined with QZZKF (10.16 g/kg/day). QZZKF and AZM were administered by oral gavage every day for five ...
Song Jiang, Dong-liang Liu, Fa-lin Zhou, Xian-bin Mo, Qi-bin Yang, Jian-hua Huang, Li-shi Yang and Shi-gui Jiang*
Ikechukwu Eugene Onah*, Ifeoma Esther Aniaku, Jude Ifeanyi Okwor and Ada Sylvester Haruna
...lic health risk. Data on plasma biochemistry profile which is indicative of the health condition of the birds in the wild is scanty while knowledge of the environmental factor that bridges the gap between the crow and man is lacking. This study evaluated the plasma biochemistry profile of C. albus and the environmental factor that bridges the gap between man and crow through which disease pathogens could be transmitte...

Khlood G. Abdelkhalek*, Aly B. A. Badawy, Mohamed Fathi, Elshymaa A. Abdelnaby 

...>Pubertal alterations in plasma levels of follicle stimulating hormone (FSH), luteinizing hormone (LH), estradiol 17β (E2), testosterone (T), and serum nitric oxide metabolites (NOMs) were measured in 5 Baladi bucks weighting (13–15 kg; with a body condition score of 3.5 ± 0.01) from 4-months- to 10-months-old in relation to testicular hemodynamics. Testicular morphometry, scrotal circumference, Doppler examinations, image analysis, and blood...
Noor Bad Shah Khattak1, Hamayun Khan1, Muhammad Tarique Tunio2, Shakoor Ahmad1, Nazir Ahmad3, Syed Murtaza Hassan Andrabi4, Obaidullah5, Ihsan Ali1,5,* and Nan-Zhu Fang5,*
...motility, survivability, plasma membrane integrity and acrosome integrity of frozen buffalo bull sperms. The antioxidative effect of GT was observed at the comparatively lower concentration (0.1-1.5%) on the post-thawed quality indicators. On the other hand, the hight GT concentration (1.5%) significantly decreased the quality indicators of spermatozoa. Green tea extract enhanced the spermatozoa survivability, integrity of acrosome and

Fang Zhao, Gen Wang, Xiaobin Li, Guodong Zhao, Hui Chen, Chen Ma and Kailun Yang*

...d melatonin (MLT) in the plasma of sheep. Eighteen sheep were assigned randomly to three diet groups (n = 6). The treatment groups included control (CT, corn-soybean meal basal diet), CT + 111 (CT + 111 mg/kg BW RPT 5-HTP), and CT + 222 (CT + 222 mg/kg BW RPT 5-HTP) groups. The experiment lasted for 16 d. On the 16th day of the experiment, blood samples from the sheep were collected at 0 h before feeding and at 1.5, 3, 4.5, 6, 8, 10, and 12 h after feeding in ...

Shireen S. Aboelwafa¹, Alsagher O. Ali², Rania Hamada³, Hassan Y.A.H. Mahmoud2* 

...t, ELISA 

...

Fulencio Ferretiz-Rodríguez1, Jose Alejandro Roque-Jiménez1, Héctor A. Lee-Rangel1*, Gregorio Alvarez-Fuentes1, Juan Carlos Garcia-Lopez1, Rolando Rojo-Rubio2 

Alexander Tsybulsky1*, Tatyana Tabakaeva1, Anna Klimovich2, Michail Shchelkanov1, Eduard Kostetsky1, Maksim Aliev1, Anton Degtyarenko3 

...fic antigen in the blood plasma; 5) Expression of ifnα6, ifnα7, ifnβ1, ifnγ and ifnλ1 genes in WBC; 6) Expression of p53 and gadd45g genes in WBC. The concentration of the viral p27-FeLV antigen in the blood plasma was determined by ELISA. The total DNA and total RNA was extracted from the WBC. The cDNA was obtained using an mRNA matrix. Qualitative and quantitative analyses of FeLV in WBC as we...

Hung Van Mai1, Tuan Duc Nguyen2, Nang Thu Tran Thi3 and Hung Phuc Nguyen4*

...estion status, including plasma lipid level, liver and muscle lipid contents, bile acid (BA) concentration, lipase activity, and lipid apparent digestibility coefficient (ADC) in pompano. The FSBM1 was produced by fermentation of defatted soybean meal (SBM) with Bacillus subtilis B3. The FSBM2 and FSBM3 were commercially available products, which were produced by fermentation of SBM with Lactobacillus spp. Five diets were formulated and denoted as FMD (fish me...

Wafaa M.A. Ghoneem, Reham R. El-Tanany and Adel E.M. Mahmoud*

...eased. Concentrations of plasma urea were significantly (P<0.05) decreased with lambs fed zeolite rations. Results indicated that zeolite addition had no adverse impact on lambs’ health which all blood parameters were within the normal range. Lambs fed 2% zeolite ration had the highest ADG and the best feed conversion and energy utilization efficiency with no significant difference among the different groups. Also, the addition of 2% zeolite decreased...
Muhammad Tariq1, Imtiaz Rabbani2, Muhammad Shahbaz Yousaf2, Imdad Ullah Khan1*, Hafsa Zaneb2, Sajid Iqbal3, Alam Zeb Khan1, Shakirullah1, Muhammad Shuaib Khan1, Atta Ur Rehman1 and Mumtaz Ali Khan2
...ere percentage motility, plasma membrane integrity (HOST assay), acrosomal integrity (NAR), viability (Live/Dead), DNA integrity (Acridine orange assay) and oxidative stress (TBARS assay). Five straws from each Gallic acid group were thawed individually in water bath at 37°C for 30 seconds. The addition of 15 µM GA to semen extender improved marginally the buffalo bull spermatozoa motility, viability and membrane integrity but still not sufficient to...

Nargis Sardar1*, Maria Binte Sarfraz2, Sufian Rasheed3 , AKM Rezwan Sardar4 , Fahamida Zaman5, Arsalan Rasheed6,7* 

...ood, or even human blood plasma in the detection of Vitamin D from diverse samples, including Vitamin D production in nature. Among the most commonly used electrochemical biosensors for vitamin D detection are Ab-25OHD/SPE/ FMTAD, CYP27B1/GCE, SiO2/GO/Ni(OH)2/GCE, BSA/Ab-VD2/CD-CH/ITO, BSA/Anti VD/Fe3O4 PANnFs/ITO, BSA/Ab-VD/Asp-Gd2O3NRs/ITO, 25OHD Antibody, 25OHD, 25OHD Antibody, IoT Enabled Enzyme Embossed Biosensor, Au-Pt NPs/APTES/FTO and GCN-β-CD/Au ...

Sharjeel Saif1, Asghar Ali Kamboh1*, Ghulam Mustafa Solangi2, Rehana Burriro3, Hasina Baloch1, Atta Muhammad Memon2  

..., Survey 

...

Hamdy Abdala Elnagar*, Wael Mohamed Wafa, Moataz Ibrahim Badwy, Abdelaziz Mustafa Sakr 

...lationship between blood plasma (BP) proteins and seminal plasma (SP) proteins in low and high fertile buffalo-bulls. Also, the relationship between protein profile in BP of adult bulls and bull calves were studied. Blood samples were taken from 3 calves (128.33±7.58 kg and ageing 6 mo.) and 10 bulls (400±37.5 kg and 24-25 months of age) and semen samples from bulls. Semen was collected once a week from all bul...

Amira M. Refaie1*, Manal S. Mohamed1, Enayat H. Abo El-Azayem1, Sherein H. Mohamed2 

...yde (MDA) level in blood plasma by increasing AA levels in diets. None of the groups record any significant differences in immunoglobulin (IgM and IgA). It could be concluded that adding Aspergillus awamori to does diets up to 0.15% enhanced their performance during lactation period, in term of increased their milk production and litter weight at weaning.

Keywords | Aspergillus awamori , Doe rabbits, Milk production, Productive performance, Weaning bun...

Mona M. Osman1, Kamelia M. Osman2, Manal Abu Elmakarem Mohamed1, Mahmoud E. Hashad2, Jeeser Alves Almeida3, Alaa Saad4, Octavio Luiz Franco5,6, Heba N. Deif 2* 

...the pathogenicity of Mycoplasma arginini (M. arginini) and Mycoplasma ovipneumoniae (M. ovipneumoniae). It shed light on the current knowledge gap in the role of two potential virulence determinants viz H2S production and catalase activity, for their possible roles in biofilm formation, antibiotic resistance and hemolytic activity of M. ovipneumoniae. The recovered sheep isolates of M. arginini and M. ovipneumoniae were exam...

Basanta Kumar Adhikari1*, Deepak Subedi1*, Sumit Jyoti1, Krishna Kaphle2, Chet Narayan Kharel3, Doj Raj Khanal4

...f antibodies against Mycoplasma capricolum capripneumoniae. Out of the total serum sample tested, 3 were seropositive for CCPP giving an overall apparent seroprevalence of 3.37% and true seroprevalence of 3.4%. Significantly higher seroprevalence (p<0.05) was observed among goats with a history of respiratory symptoms. This study revealed the goat population in Rupandehi (district bordering India) and Palpa districts are at high risk of acquiring CCPP infec...
Roy Hendroko Setyobudi1, Erkata Yandri2, Yogo Adi Nugroho3, Mardiana Sri Susanti4
Satriyo Krido Wahono5, Wahyu Widodo1, Lili Zalizar1, Elfi Anis Saati1, Maftuchah Maftuchah1
Manar Fayiz Mousa Atoum6, Muhannad Illayan Massadeh6, Dwi Yono3, Rangga Kala Mahaswa7
Herry Susanto2, Damat Damat1*, Dyah Roeswitawati1, Praptiningsih Gamawati Adinurani8 and 
Susi Mindarti1

Aiman Al Mufarji, Abd El-Nasser Ahmed Mohammed* 

... modulation of blood and plasma parameters of ewes in subtropics. Twenty seven Naimi ewes, 51.3 ± 2.70 kg body weight and aged 2.5 - 3.0 years, were randomly allocated to three equal groups (nine/group) as control and M. oleifera groups (25 & 50.0 g daily). Blood samples were collected and were subjected to analysis through hematology and plasma biochemistry analyzers. The hematological parameters include red and ...

Zahra Jamilah Sabrina1, Adrian Pearl Gunawan2, Beryl Reinaldo Chandra2, Ilham Pangestu Harwoko3, Johannes Marulitua Nainggolan4,Widi Nugroho1* 

Abdelrahman A. Abdelrahman1, Salama A. S. Shany1, Kareem E. Hassan1, Mansy A. A. Dardeer2, Ali A.1*, Magdy F. El-Kady1* 

...tis virus (IBV), and Mycoplasma spp. are high. Thus, co-infection with one or both viruses and Mycoplasma gallisepticum (MG) or Mycoplasma synovaie (MS) is thought to play an important role in the clinical severity of respiratory infections. In this study, we conducted a passive surveillance study for MG and MS among cases of viral respiratory affections in broilers. Samples were collected...

Hamdy Abdala Elnagar, Wael Mohamed Wafa*, Abdelaziz Mustafa Sakr, Mohammed Mahmoud Hegazy 

...were determined in blood plasma. Results showed insignificant increase in TES and PRL by age advancing at 1-3 month, and in leptin at 1-6 month of age. All hormones markedly increased up to 24 month of age. TES increased by increasing body weight (BW) from 40 to 420 kg. Leptin and PRL increased by increasing BW (>200 and >120 kg, respectively). The polynomial regression equation for all hormones gave the highest R2 values 0.889 and 0.971 for the unique m...
Aiman Al Mufarji1, Abd El-Nasser Ahmed Mohammed1*, Rashid Al Zeidi2, Haitham Al Masruri3
...tological parameters and plasma biochemistry parameters. The results indicated that heat stress negatively influenced the thermo-tolerance, hematological, and plasma biochemistry parameters. M. oleifera alleviated the negative effects of heat stress, as well as modulated thermo-tolerance responses, metabolites, and liver and kidney functions. M. oleifera supplementation with a rate of 50 and 100 g to stressed ewes especially...

Yasmin M. M. Mahmoud* 

...th control one. In blood plasma, concentrations of total protein, albumin and globulin as well as activity of aspartate aminotransferase (AST) and alanine aminotransferase (ALT) were significantly (P<0.05) increased while, glucose, total cholesterol, uric acid and creatinine were significantly decreased (P<0.05) in G1 as compared to unsupplemented group. Kids in G1 group recorded the highest (P<0.05) concentrations of hemoglobin, mean corpuscular volu...
M. Fatmawati1, L.T. Suwanti2*, Mufasirin3
...ic disease caused by Toxoplasma gondii. The definitive host of T. gondii is the cat, while the intermediate hosts are all mammals. Livestock become infected by ingesting sporulated oocysts in the environment, while humans can be infected by T. gondii due to environmental contamination by T. gondii oocysts, consumption of foods and milk containing cysts or tachyzoites of T. gondii. Raw milk is a risk factor for toxoplasmosis in humans. This paper discusses the ...

Hui Xu1,*, Ronghua Luo1, Yaping Duan1, Mingjia Chen1, Jiaxu Zhou1 and Xiaoli Shao2

...ood in children with Mycoplasma pneumoniae pneumonia (MPP). From January 2018 to January 2020, 114 children with MPP in our hospital were selected and randomly divided into control group and experimental group, each containing 57 cases. The control group was given azithromycin. The experimental group was given fibrobronchoscope-assisted lavage on the basis of the control group. The curative effect, the time of remission of clinical symptoms (pulmonary rales, b...

Abdullah Channo1*, Asmatullah Kaka1, Qudratullah Kalwar2, Imdadullah Jamali3, Ghulam Jelani2, Muhammad Bakhsh4, Ghulam Nabi Dahri5 and Jai Parkash Goil6

...of progesterone level in plasma and milk by using kits of progesterone assay, and to confirm the diagnosis of COD, mostly trans rectal palpation and trans rectal ultrasonography methods have been used. COD should be treated by using different treatment protocols such as hormonal, medicinal and homeopathic medicines.

...

Muhammad Hammad Fayyaz*, S. Murtaza Hassan Andrabi and Muhammad Shafiq Haider

...g, progressive motility, plasma membrane integrity (PMI), and livability of buck spermatozoa were higher (P< 0.05) with 1mM GLU and 10mM CYS as compared to control. In the second experiment, post-thaw progressive motility, PMI, and livability were higher (P< 0.05) with 1mM GLU + 10mM CYS and 1mM GLU + 5mM CYS compared to other doses of GLU+CYS and control. We concluded that optimized supplementation of Glutathione and L-cysteine in TCA extender ameliorat...

Tinda Afriani*, Zaituni Udin, James Hellyward, Endang Purwati, Adisti Rastosari, Dwiki Wahyudi 

...percentage of motility, plasma membrane integrity (PMI) and acrosome intact (AI). The result revealed that the average motility, PMI and AI of sperms bearing X- and Y-chromosome in the albumin gradient method was 58.33±10.32% and 60.83± 13.57%, 53.63±16.54% and 52.76± 8.88%, and 53.00±7.93% and 54.08±8.76%, respectively. In swim-up method the average motility, PMI and AI of sperms bearing X- and Y-chromosome was re...

Arsalan Maqbool1,2, Faez Firdaus Abdullah Jesse1,3*, Eric Lim Teik Chung3,5, Abd Wahid Haron3, Mohd Azmi Mohd Lila5, Bura Thlama Paul3,4, Khaliq ur Rehman Bhutto3,6 

 

...esults revealed that the plasma progesterone concentration increased significantly (p<0.05) whereas estrogen, follicle-stimulating hormone and luteinizing hormone decreased significantly (p<0.05) in the non-vaccinated group compared to vaccinated and control groups in both seasons. The study outcomes shown that climate has neither significant effect on reproductive physiology nor exaggerated the effect on an experimentally infected animal. Furthermore, i...

Liyun Chang1, Aiju Liu2, Jianshuang Zhang3, Yingbin Chen1 and Zhiyong Liu4*

... on the SAG2 gene of Toxoplasma gondii (T. gondii) to improve the detection of toxoplasmosis in pet cats. Escherichia coli BL21 (DE3) was transformed with a prokaryotic expression vector, pET-21a-SAG2, which was constructed and induced to express a recombinant protein (SAG2), identified using SDS-PAGE and Western blot analysis. The purified protein was then used as a coating antigen to establish an indirect ELISA method for detecting the T. gondii antibody in ...

Eman A. Al-Shahari1,2, Eman R. ElBealy3, Abdelhalim A. Alkhazendar4 and Abeer A. Alm-Eldeen4*

...crease in the liver ALT, plasma cholesterol and MDA comparing with 3 months old rats. The percentages of p53 and bax positive areas/ field were 26.04% and 24.9%, respectively. After quercetin administration, 30 months old rats showed non-significant change in the previous biochemical parameters compared with 3 months old rats and the percentages of p53 and bax positive areas/ field were 0.6% and 0.9%, respectively. In conclusion, quercetin at a dose of 200 mg/...

Hanan Saad El-Samahy, Amani Abd El-Naby Hafez, Mohamed Talat Ragab, Disouky Mohamed Mourad*  

...chia coli (E. coli), Mycoplasma gallisepticum (M. gallisepticum), Mycoplasma meleagridis (M. meleagradis), Ornithobacterium rhinotracheale (ORT), Pasteurella multocida (P. multocida), and Pseudomonas aeruginosa (P. aeruginosa) . The most prevalent detected bacteria were E coli (13) followed by M. gallisepticum (12), ORT (12) and M. meleagridis (7). Two farms reported single infection, one with M. gallisepticum and one with E...

Mutassim Abdelrahman1, Ibrahim Alhidary1, Majdi Bahaddi1,2, Mohsen Alobre1,3, Riyadh Aljumaah1 and Rifat Ullah Khan4, *

...s by inductively coupled plasma optical emission spectrometry. Results indicate that there is no significant difference (P >0.05) between the concentration of all the elements in all lambs’ tissues, except for the concentration of aluminum in kidney and rumen fluid, as well as Mn in rumen fluid. Moreover, Mn concentration in rumen fluid was significantly (P < 0.05) decrease with zeolite supplementation, with significantly lower values for lambs fro...
Abdulaziz A. Alaqil1*, Mohammed I. Buhaya2
...d blood hematological or plasma biochemical parameters of the layers. It can be concluded that inclusion of LO into layer’s diets can be safely used to enhance egg production. Furthermore, LO inclusion might have a beneficial impact on reducing egg cholesterol content which can be considered as an adding value for consumer’s general health. The economic efficiency evaluation reviled that diet fortified by 6% LO is the most efficient diet in respect...

Wael Mohamed Wafa1*, Mohammed Mahmoud Hegazy1, Mohamed Mohamed El-said Ibrahim1, Hamdy Abdala El-Nagar1, Rehab Fawzy Ismail2 

...bin, packed cell volume, plasma total proteins, globulin, glucose, total cholesterol, total lipids, and triglycerides increased (P<0.05), while those of urea-N, creatinine, and aspartate and alanine transaminases decreased (P<0.05) in GG and PFG compared with CG. Ages at puberty, 1st service, and conception were earlier (P<0.05) in GG and PFG than in CG. Pregnancy rate was the highest in GG (100%), followed by PFG (83.3%), and the lowest in CG (66.7%...

Ibrahim S. Abu-Alya1*, Nagwa I. Sheraiba2, Enas K. Aziz2, Noha A. Osman2, Afaf A. Kishta1 

...overall body weight. The plasma biochemical parameters results indicate that garlic oil and/or ginger oil supplementation on the Nile tilapia diet at different levels raises markedly raises the T4 hormone level and reduces total cholesterol and LDL-cholesterol levels. Moreover, administration of 1% garlic oil and ginger oil (T3 and T5 respectively) and both of them (T6) to the diet of Nile tilapia significantly (P<0.05) reduces the ...

Ekechukwu Esther1, Ohanu Chinenye1, Ossai Nelson1*, Elijah Okwuonu1, Hinmikaiye Funmilayo1, Ngene Innocent1, Andong Felix1, Ikegbunam Clara2, Echude Daniel1, Ekeh Felicia1 and Odo Gregory1

... C. cajan. The values of plasma total protein, albumin, Albumin/Globulin ratio, glucose, total cholesterol and urea nitrogen revealed non- significant changes among the groups that received different dietary treatments (P>0.5). The activities of small intestine trypsin of rats fed on diet 20% were higher than those of other diets, (p<0.05). The rats fed on 50% diet had the highest relative weight of stomach while the ones fed on 0% diet had also the high...

Saleh M. Albarrak1*, Fahad S. Aldahmashi1,2, Abdulrhman A. Alrubayan1,2, Abdel Kader A. Zaki1,3 

...in the serum and seminal plasma of 29 infertile dromedary male camels, which were of two different breeds (Waddah & Majaheem) and three different ages (> 3 to ≤5, ≥5 to ≤7, and >7 years). ASAs were detected in ≥ 80% of the examined animals with considerable individual variation within each group. The serum and seminal plasma ASAs indexes (%) were significantly elevated in the >7 years group compared ...
Nuzhat Kamal1, Muhammed Sajjad Ansari2*, Bushra Allah Rakha1, Shamim Akhter1, Saima Qadeer1 and Ali Akhter1
... for sperm motility (%), plasma membrane integrity (%), viability (%), normal apical ridge (%) and chromatin integrity (%). Sperm motility was recorded higher in extender containing 15mM alanine compared to control. Sperm viability was recorded higher in extender containing alanine 10, 15, 20mM compared to control. Sperm plasma membrane integrity, normal apical ridge and chromatin integrity remain similar in all experimental...

K. H. El-Kholy1*, Enayat Hassan Abo El-Azayem2, Safaa Ataya Barakat2, Mona Gamel Mohamed3, Sara H. M. Hassab1 

...creased. Haematological, plasma biochemical and antioxidant enzymes (SOD, GPx and CAT) were significantly elevated (P≤0.05) by the highest dietary AV inclusion (G4). The addition of AV significantly enhanced immune- responses; lymphocyte percentage, IgG and IgM increased when growing rabbits were fed G4 compared to the control group. In conclusion, dietary addition of 1 or 2g AV may serve as an enhancer of growth and immune response in growing rabbits.

...

Kirana D.D. Adiputra1*, Tulus Maulana3 , Ekayanti M. Kaiin3, H. Hasbi2, Herry Sonjaya2 

...ity, abnormality, intact plasma membrane, and concentration. Meanwhile, parameters observed in frozen semen were motility, viability, abnormality, intact plasma membrane, acrosome status, DNA integrity, and movement patterns. Viability and abnormality parameters were stained with eosin-nigrosine, intact plasma membrane was stained with Hypo-osmotic (HOS) solution, acrosome status was stain...

Muhammad Bilal Munawar1, Saima Naveed1, Shafqat Nawaz Qaisrani1, Jibran Hussain2, Shahzad Munir3, Ayesha Ahmed3, Mubbashir Hussain4, Ahmad Kamran Khan5, Abdullah Jalal6 and Irfan Ahmed7*

...is of growth production, plasma and tibia phosphorus. The (n=504) day-old broiler chickens procured randomly allocated into 6 treatments dietary treatments were: B positive control (PC) diet (0.42% available phosphorus in grower feed, A negative control (NC) diet with (0.30% avail. P in starter and 0.26% avail. phosphorus in grower) while four diets C, D supplemented with NC+ (500 and 750 powder phytase units (FTU)/kg) and G, H supplemented with NC+ (500 and 7...

Ibrahim El-Ratel1*, Mostafa El-Moghazy2, Amira El-Gaml2, Ibrahim Abu El-Naser2 

...ood serum and in seminal plasma. Advanced motility, membrane integrity, livability and sperm viability were improved, but sperm abnormality, apoptosis and necrosis were reduced in cryopreserved semen by all PEE levels. Total antioxidants capacity was increased, while malondialdehyde was decreased in post-thawed semen by all PEE levels. Conception rate of doe rabbits was improved by artificial insemination with fresh and thawed buck semen with all PEE levels, b...

Ibrahim Samir Abd El-Hamid1, Alaa Emara Rabee2, Moustafa Mohamed M. A. Ghandour2, Rasha Salah Mohammed3, Ahmed Mohamed. Sallam4 

Mustafa Erkan Özgür1*, Selim Erdogan2, Songül Aydemir3 and Hatice Yumusakbas2

...sitions of semen seminal plasma in rainbow trout (Oncorhynchus mykiss) and brown trout (Salmo trutta fario) were analyzed in fresh semen by gas chromatography (GC) and investigated the relationships with the sperm cell velocities. According to our results, total levels of saturated fatty acids (SFA) and monounsaturated fatty acids (MUFA) for Salmo trutta fario occurred in higher quantities than Oncorhynchus mykiss but the quantities of total polyunsaturated fa...
Makruf Arif1*, Claude Mona Airin1, Dwi Sunu Datrianto2, Dinda Anggun Roro Sejati3
...viability, and damage to plasma membranes that can reduce fertility rates. The use of antioxidants in semen preservation is very important to suppress lipid peroxidation so it can maintain the quality of the sperm. This study aimed to determine additional honey to the quality of KUB rooster sperm during cold storage. The semen with superior quality was diluted into four different treatments, lactate ringer egg yolk without honey as control (LREY0),   ...
Farid S. Nassar1,2, Abdulaziz M. Alsahlawi3, Ahmed O. Abbas1,2*, Abdulaziz A. Alaqil1, Nancy N. Kamel4, Abdelwahab M. Abdelwahab1,5
...erol, and calcium in the plasma. Furthermore, a linear increasing effect (p < 0.05) on the T3 and E2 hormone concentrations and on the humoral and cellular immune response were obtained as the dietary BSFL level increased. Moreover, BSFL treatment linearly increased the profit margin, the cost benefit ratio and the return on investment per bird, recording the highest economic efficiency when employing 12% BSFL in the layer diets. Our results conclude that e...
Shaker Al-Suwaiegh
...ted for determination of plasma proteins, glucose, liver enzymes and minerals parameters. The results indicated that Azolla pinnata contain protein (32.83%), crude fiber (13.6%), ether extract (1.17%) and ash (3.89%). Upon feeding, replacement diet with 20.0% Azolla pinnata caused significant increase in feed efficiency and body weight gain. In addition, the plasma metabolites (total protein, globulin, and urea) and minerals...

Aiman Al-Mufarji, Shaker Al-Suwaiegh, Abd El-Nasser Mohammed*

...ls, and lymphocytes) and plasma parameters (total protein, albumin, globulin, glucose, urea, and minerals) of ewes and lambs. It could be concluded that M. oleifera leaves supplementation to pregnant ewes from eight weeks prepartum to eight weeks postpartum might be ameliorative for both productive and reproductive performances of ewes through modulating thermo-tolerance responses, blood and plasma parameters in subtropics.<...

Athhar Manabi Diansyah1, Muhammad Yusuf2*, Abdul Latief Toleng2, Muhammad Ihsan Andi Dagong2, Tulus Maulana3, Hasrin4, Abdullah Baharun5 

...ng quality and proteomic plasma semen as fertility performance in Bali-polled bull. The utilization of Bali-horned bulls as a point of reference was necessary to achieve this aim. The semen samples from Bali-horned and Bali-polled bulls were obtained twice weekly using an artificial vagina. The semen samples were immediately sent to the laboratory for processing. Parameters measured were sperm motility, abnormality, viability, acrosome integrity, and membrane ...

Ghulam Jelani1, Muhammad Haris Raza Farhan2, Rida Asrar3, Muhammad Ahmad1,4*, Chanda Naseem5, Zafar Ali Khoso6, Majid Hussain Soomro7* 

ASIMA BANO1, HAFIZ MUHAMMAD TAHIR*2, AROOSA RASHEED3, MUHAMMAD ARSHAD4, SHAFAAT YAR KHAN1 & RABIA YAQOOB5

...ion in breast cancer and plasma levels of non-specific esterases in breast cancer patients. For study blood samples were collected from various diagnostic laboratories of Sargodha city. Levels of non-specific esterases were determined in the normal and breast cancer patients. The levels of non-specific esterases were higher in healthy individuals than the breast cancer patients. So, levels of non-specific esterases could be used as a marker for the diagnosis o...
ASMA ABDUL LATIF*1, SAMREEN MUSHTAQ1, SABIHA FAZAL1, MUHAMMAD MANSHA2, & ATIF YAQUB3
...opositive.
...
 
 MUHAMMAD MUNEEB SOHAIL1, FATIMA AMEER2, GUR CHARN SINGH2, MUHAMMAD ZAID1* 
... concentrations of other plasma lipids. This study reanalyzes the relation between plasma lipids and various anthropometric parameters. In this cross-sectional type of study, the participants (n=53) were recruited for the study. Participants were given one-day free health camps and they were anthropometrically measured. Plasma lipid fractions were accordingly determined. Participants suffe...

Tinda Afriani*, Jaswandi, Adisti Rastosari, Muhammad Cadilac Al Razzak, Dwiki Wahyudi 

...abnormalities and intact plasma membranes of spermatozoa. The obtained data was analyzed using the Analysis of Variance (ANOVA). The results showed that the addition of tomato juice extract into egg yolk citrate diluent exhibited a 20% increase in sperm motility percentage, 26.3% in sperm survival rate and 22.5% in intact plasma membrane percentage as compared to the control group (P < 0.01). However, the sperms abnormali...

Doaa H. Assar1, Rasha A. Al-Wakeel2, Zizy I. Elbialy3, Mahmoud M. El-Maghraby4, Helmy K. Zaghlool5, Adel A. El-Badawy4, Abdel-Khalek E. Abdel-Khalek6*

...PL. In serum and seminal plasma, glutathione, superoxide dismutase, catalase, and glutathione peroxidase significantly increased, while malondialdehyde significantly decreased in SPL groups. Based on our results, spirulina as a dietary feed additive (5 g/kg diet) could be used safely for improving growth, health, antioxidant defense system, and semen quality of growing cross-bred ram lambs from 10 to 15 months of age.
 
Keywords | ...

Hasanain Jihad Neamah 

... improve sperm motility, plasma membrane integrity, and dead sperm percentage at cooling preservation. 

...

Om-Hashem M. El Banaa l, A.A. Abou Zeid2, Fawzia I. Moursy3 and Azza, G. Farag

...xt-align: justify;">Spiroplasma citri Saglio et al is the type species of the genus spiroplasma. Spiralin gene is the gene responsible for spiralin which is the major protein of Spiroplasma citri (S. citri Saglio et al), the causal organism of citrus stubborn disease. RecA gene protein is the enzyme primarily responsible for the homologous recombination of chromosomal DNA in bacteria. The ...

Rahma Boukarine*, Leila Hamdi and Kamel Khelili

...uric acid and creatinine plasma levels. Additionally, a significant decrease in GSH levels and GPx activity accompanied with an increase in MDA levels were noted due to the exposition of xylene. However, animals that received ESAE with xylene (REX1 and REX2) showed an adjustment of these perturbations, by a significant decrease in the levels of AST, ALT, ALP, urea, uric acid, creatinine and MDA. As well as an increase in GSH levels and GPx activity compared to...

Lalu Ahmad Zaenuri*, Rodiah Rodiah, Adji Santoso Dradjat, Oscar Yanuarianto, I Wayan Lanus Sumadiasa , Lukman Hy 

...oncentration/ejaculates, plasma membrane integrity, viability, progressive motility, and morphology, including normal and abnormal spermatozoa. The statistical significance of the results was evaluated by pre- and post-test analyses using T-test with repeated measuring. Data were expressed as Means ± SD and SEM. Results revealed that semen volume, spermatozoa concentration/ejaculate, plasma membrane integrity, viabili...

Sigit Bintara1*, Riyan Nugroho Aji1, Panjono2, Ali Agus3

... post thawing (60. 14%); plasma membrane integrity before freezing (71.29%) and post thawing (59.86%); and also showed the lowest abnormality level before freezing and post-thawing, (17.29%) and (24.14%), respectively.
 
Keywords | Spermatozoa, Belgian blue crossbreed, Egg yolk citrate, Lycopene
...

Sarwat Yousuf1, Shaista Emad2 , Mohammad Misbah ur Rehman3, Zehra Batool4, Sara Qadeer5, Yousra Sarfaraz1, Sheeza Sheikh1, Sana Sadaf6 and Tahira Perveen1,*

...re also enhanced whereas plasma corticosterone levels were significantly decreased following the administration of a low dose of lemon peel oil. However, rats administered with higher doses of lemon peel oil that act as prooxidants showed depression and anxiety-like effects, and impaired muscular strength. Altered brain antioxidant enzyme activity and elevated corticosterone in plasma were also observed in rats treated with ...

Luigi Liotta1, Vincenzo Lopreiato1, Fariborz Asroosh2 and Alireza Seidavi2*

...sing inductively coupled plasma mass spectrometry. Data were subjected to the two-way ANOVA analysis to determine the significant differences between species/breeds. The acidity, density and fat in Talesh sheep’s milk was higher than milk from other species/breeds (P<0.01). Also, the amount of protein in Talesh sheep’s milk was significantly higher (P<0.05) than all animals tested with the exception of Talesh buffalo milk. The pH of Saanen go...

Abdulaziz A. Alaqil*

...rature (BT), H/L ratio, plasma malondialdehyde (MDA), and corticosterone (CORT) concentrations were evaluated in the treatment groups (n=10). Additionally, samples of the spleen and duodenum were taken from layers in each group (n=10) to assess heat shock protein 70 (HSP70) density and glutathione peroxidase (GHS-px) activity, respectively. The general linear model procedure with one-way analysis of variance (GLM-ANOVA) was used to analyze the data. The ...

Supawadee Piratae1*, Noraphat Khiewkham2, Nattawut Maungmungkun2, Chanakan Tippornwong2, Tossapol Seerintra2, Sirikanda Thanasuwan3, Luyen Thi Phung4 

...p>Ehrlichia canis and Anaplasma platys are rickettsial pathogens that cause infections in the blood and have adverse health effects in animals, especially dogs and other canids. PCR targeting the 16S rRNA gene was performed to determine the prevalence of ehrlichiosis and anaplasmosis in 127 dogs collected from Rayong province, Thailand. To confirm the identification of these two pathogens, PCR was performed to detect the citrate synthase (gltA) gene followed b...

Vui Van Nguyen1, Samorn Ponchunchoovong2, Sajeera Kupittayanant3, Pakanit Kupittayanant2* 

...ne potential, the intact plasma membrane and acrosome membrane indicators in the Ocimum gratissimum essential oils treatment were higher than those in the rest treatments and had a substantial difference when compared to the control treatment (P<0.05). In concluding, semen extender supplemented with Ocimum gratissimum essential oils is appropriate for improving the chilled canine sperm for the duration of 12 days in storage. 

...

Kazi Afsana Homayra Orchy1, Mst. Antora Akter1, Nelema Yesmin1, Md. Moshiur Rahman Khan1, Marzia Rahman2, Md. Mahmudul Alam1* 

...terologous platelet-rich plasma (hPRP) in the treatment of third-degree burn wounds. A total of 18 circular third-degree burn wounds measuring 10mm in diameter were made in six rabbits and studied under two groups: Group A and Group B. The wounds of Group A were treated topically with freshly prepared hPRP gel twice a week. The control rabbits received normal saline topically. The wounds were evaluated for 45 days post-burning. Morphological features of wounds...

Walaa I. Mohameden1, Haidy G. Abdel-Rahman2, Ibrahim M. Hegab3* 

...ored alongside blood and plasma samples were harvested at days 0, 30, and 60 of the study for hematological and biochemical analysis. Results revealed an overall improvement in animal health, skin conditions, and liver and kidney functions. Significant (P < 0.05) increases in plasma proteins, zinc, and copper were recorded, while malondialdehyde, TNF-α, and MMP-9 significantly (P < 0.05) decreased. Furthermore, b...

Yingying Wang, Jianyi Yu and Lichen Zhou*

...ptibly infected with Toxoplasma gondii. In this study, we used polymerase chain reaction to detect DNA, and enzyme-linked immunosorbent assay to test for T. gondii antibodies. Antibodies (S/P>0.31) to T. gondii were found in 20 (26.32%) of the 76 tigers, while all blood samples tested through nested PCR were negative. This is the first investigation of T. gondii infection in South China tiger.

...

Kiran Afshan1*, Sobia Baseer1, Shanza Kiran1, Ghulam Narjis2 and Sabika Firasat1

...ext-align: justify;">Toxoplasma gondii is a globally found intracellular parasite that infected a large proportion of the world population, it remained asymptomatic in immunocompetent patients but the acquisition of the infection during pregnancy can lead to abortions and other congenital defects. The present study aimed to find the seroprevalence of T. gondii among suspected women who visited local hospitals in Khyber Pakhtunkhwa (KP), Pakistan. Sera of 425 s...

Moustafa M. Zeitoun 1,3; Mohamed M. Mansour 2,4* 

...dy the blood and seminal plasma aldosterone and testosterone levels of male dromedary camels and their outcome on semen parameters and fertility. Methods; From breeding history records, depending on fertility status, sixty camels had divided into two groups; control “fertile” (n = 20) and infertile (n = 40). Blood and seminal plasma have obtained from all camels for hormone analysis. Results; Blood testosterone l...

Ijaz Ahmad1,5*, Haihong Hao1, Pascal Sanders2, Zafar Iqbal3, Saeed Ahmed4, Farhan Anwar Khan5, Zahir Shah5, Muhammad Ibrahim6 and Lingli Huang1

... cefquinome samples from plasma were extracted and separated by HPLC on a reversed phase C-18 column with mobile phase of acetonitrile and formic acid (0.1%) solution- (90:10, v/v). The calibration curves were linear ranged from 0.01-5µg/mL. The limit of quantification (LOQ) and limit of detection (LOD) were 0.04 and 0.01 µg/mL. The recovery of cefquinome from plasma was 73.4%, 78.33%, and 77% for low, medium and...

Imbang Dwi Rahayu1, Ali Mahmud1*, Wahyu Widodo1, Adi Sutanto1, Apriliana Devi Anggraini1, Devi Dwi Siskawardani2, Wisnu Nurcahyo3, Tri Untari3

...ct (p>0.05) the total plasma protein (TPP), globulin, cholesterol, high-density lipoprotein (HDL), aspartate aminotransferase (AST), and all parameters of the blood profile. However, the herbs’ addition significantly (p<0.05) affected albumin, triglycerides (TG), low-density lipoprotein (LDL), alanine aminotransferase (ALT), and malondialdehyde (MDA). Adding herbs through drinking water gave lower TG and LDL levels than through feed or the control....

Reza Esmaealzade Dizaji1, Arasb Dabbagh Moghaddam1*, Arash Ghalyanchi Langeroudi2, Mohamad Foad Heydari3, Seyyed Javad Hosseini Shokouh4 and Ana Shirzad Shahrivar5

...oaded with platelet-rich plasma (PRP) was used to examine the effects on a second-degree heat wound and the healing process’ sub-streaming molecular route for re-epithelialization. Model (deep second-degree thermal wound), PRP, and PRP/BNF groups were each randomly assigned a set of animals. Wounds were monitored daily for many days following therapy to see how they were healing. H&E staining and qRT-PCR were used to examine the pathological alterati...

Omar Berkani1*, Souheila Slimani2, Nora Sakhraoui3 and Cherif Abdennour1

...erent semen analysis and plasma FT4 level were measured at week 10. Results showed that under long photoperiod, the sexual activity of the control group lasted for 06 weeks, characterized by a significant growth in the testicular volume, followed by spontaneous gonadal regression up to week 10. Azoospermia in control birds was noticed, with a significant increase in FT4 level and a fall of flying feathers in the 8th and 10th weeks, which assert the testicular ...

Syed Shakeel Shah1, Ayesha Jameel1, Sabila Afzal1*, Muhammad Zubair2, Iram Fatima Bokhari1

... (2.29%). Trichuris, Toxoplasma, Entamoeba, Fasciola, Hookworm and Giardia showed less than 5% prevalence. Statistical inquiry indicated a significant relationship (P < 0.05) of parasitic contaminations and raw vegetables. This study infers that raw and unwashed vegetable consumption is an essential route of parasitic infections to humans.

...

SAGHIR AHMAD*, WARDA HANIF & SANA YOUNAS

...tibodies specific to Toxoplasma gondii (T. gondii) simultaneously in sheep, goat and humans associated with the sheep and goat farming from Bahawalpur, Pakistan from May, 2016 to April 2017. 640 blood samples were taken from sheep, goats, farmers and non-farmer humans (160 each). Blood sera were examined to detect anti-Toxoplasma antibodies (IgG) by employing latex agglutination test (LAT). The findings showed the seropreval...

Jyotsana Shakkarpude1*, Aditya Mishra2, Deepika D. Caesar2, R.K. Sharma3, Anand Kumar Jain2, Sanju Mandal2, Rajesh Kumar Vandre4, Danveer S. Yadav5, Bhavna Ahirwar6 and C.P. Solanki6

...e overall mean values of plasma superoxide dismutase, total antioxidant capacity and immunoglobin G of high betaine group was numerically higher than other groups which differed (p<0.05) significantly between groups. The overall mean value of plasma HSP70 of high betaine group was minimum followed by low betaine group and control group had maximum mean value which differed (p<0.05) significantly between groups. The ove...

Nguyen Thi Thu Hien1, Dong Huu Rin2, Nguyen Xuan Hoa1, Le Viet Tuan Khanh2, Phung Thang Long1*, Dinh Thi Bich Lan1

...justify;">Keywords | Mycoplasma hyopneumoniae, porcine enzootic pneumonia, P36 protein, protein expression, recombinant protein, antigenicity
...

Hamdy Abdala El-Nagar1, Abdelaziz Mohamed Elhais2, Ayman Hassan Abd El-Aziz3, Wael Mohamed Wafa1, Mohamed Sobhy Elsayed Farrag2, Moataz Ibrahim Badawy1, Safaa Elsayed Salah Atia2

Abd-Alfattah Abd-Alhamid Alderey1, Nabila Elsaid Mahmod El-Kassas1, Eman Said El-Hadad1*, Manal Hamdy Gomaa1, Eman Ashour Hussein2  

... DY. Egg shape index and plasma cholesterol and triglyceride were improved (P<0.05 or P<0.001) by all SOP20% diets. Shell percentage was increased (P<0.01) by SOP20%+E+DY. Haugh unit was increased (P<0.01) by SOP15%+E or E+DY, SOP20%+E+DY. Egg surface area was improved (P<0.05) by SOP15%+E or DY, SOP20%+DY. SOP20%+DY diet showed the highest economic efficiency relative to the control followed by SOP15% or SOP20%+E diets. Overall, results demonst...

Aeni Nurlatifah1, Anita Hafid1, Pradita Iustia Sitaresmi1, Santoso Santoso1, Rahma Isatirna Anwar1, Mohammad Firdaus Hudaya1, Diana Andrianita Kusumaningrum1, Nurul Azizah1, Dimar Sari Wahyuni1, Herdis Herdis1, Florentina Bety Indah Lupitasari1, Desiana Ade Mahari1, Muhammad Rizal2

...Motility, viability, and plasma membrane integrity were the metrics used in this analysis. The experiment conducted with the additional NEZ on the 4th day of observation showed that the addition of ascorbic acid as NEZ at a dose of 20 mg/100 ml into the egg yolk tris extender resulted in the greatest preservation of ram sperm quality (P<0.05), such as motility and viability. Hence, the addition of catalase as EZ at a dose of 50 mg/100 ml to the egg yolk tri...

Yunxia Guo1, Xia Zhou1*, Xin Huang2 and Fagang Zhong2

...lus obstructivus and Mycoplasma alkalescens exhibited 100% resistance against penicillin, while Bacillus subtili and M. alkalescens showed 100% resistance against tetracycline. Mycoplasma dispar, B. subtilis, M. alkalescens, B. obstructivus, and Stah. aureus against cefoxitin; and M. alkalescens, and Microccus luteus against cefoperazone exhibited ≥90% resistance. Overall, the majority of bacterial isolates exhibited ≥...

Roheela Yasmeen1,3* , Faheem Hafeez1 , Umme Ammara1 , Rubab Younas1 , Sibtain Ahmad2 , Zulfiqar Ali3, Zaheer Ahmad Nasir4 

Karl Maramorosch

...own to be caused by phytoplasmas and spiroplasmas. Earlier attempts to concentrate, purify and visualize by electron microscopy the presumptive viruses have failed. Causative agents of yellows-type plant diseases were classified as viruses because no fungi or bacteria could be detected in diseased plants. The inadequate characterization of viruses delayed the discovery of the pathogens of yellows-type diseases by forty years...

Rizkie Elvania1, Tintin Rostini1, Sakiman2, Ani Susilawati2, Emilda Adriani2, Abd Malik1*

...tility, abnormality, and plasma membrane integrity. The findings revealed that the inclusion of 1% and 2% lycopene in the diluent before freezing exhibited significantly higher (P < 0.05) spermatozoa motility. Moreover, the viability and motility of post-thawed semen significantly improved (P < 0.05) at lycopene doses of 1% and 2%. The addition of 1% to 2% lycopene in the extender demonstrated a positive effect on the sperm qualities, particularly enhanc...

Muhammad Talha Sajjad1, Hamid Akbar1*, Muhammad Arif Khan1, Muhammad Hassan Mushtaq3, Shehla Gul Bokhari1, Muhammad Abid Hayat4 and Ghulam Mustafa2

...Autologous platelet-rich plasma (PRP) gel has been suggested for wound repair. However, there is insufficient evidence to support its usage in animals with cutaneous wounds. Therefore, this study aimed to evaluate the effect of PRP gel on cutaneous wound healing in rescued horses. Twelve horses were divided into two groups: group A (PRP-treated) and group B (control). The group A (n=6) animals were treated with autologous PRP gel, while the group B (n=6) anima...

Farid S. Nassar1,2, Osama A. El-Sayed3, Saidi Ouassaf4, Ahmed O. Abbas1,2* 

...ially (p < 0.05). The plasma total protein and triiodothyronine hormone levels were remarkably (p < 0.05) enhanced by adding FS to broiler meals. At the same time, the concentrations of alanine transferase, aspartate transferase, and uric acid were significantly (p < 0.05) lowered. Additionally, the triglycerides were dramatically (p < 0.05) decreased while the high-density lipoprotein composed a higher proportion than the low-density-lipoprotein o...

Qing Cao1, Wei Jiao2, Huihui Lu1, Jing Zhang2, Meiling Ren3, Yan Xu1* and Shuyang Hu1*

... P < 0.05); levels of plasma fibrinogen (Fb) and D-dimers (D-D) in serum of patients in the hyperglycemia group were much higher than those in the hypoglycemia group, while serum plasma prothrombin time (PT) and activated partial thromboplastin time (APTT) were much shorter than those in the hypoglycemia group (all P < 0.05). Thrombus precursor protein (TpP), P-selectin (Ps), maximum platelet aggregation rate (MAR) and...

Qing Cao1, Wei Jiao2, Huihui Lu1, Jing Zhang2, Meiling Ren3, Yan Xu1* and Shuyang Hu1*

... P < 0.05); levels of plasma fibrinogen (Fb) and D-dimers (D-D) in serum of patients in the hyperglycemia group were much higher than those in the hypoglycemia group, while serum plasma prothrombin time (PT) and activated partial thromboplastin time (APTT) were much shorter than those in the hypoglycemia group (all P < 0.05). Thrombus precursor protein (TpP), P-selectin (Ps), maximum platelet aggregation rate (MAR) and...

Khaeruddin1, Gatot Ciptadi2, Muhammad Yusuf3, Iswati4, Setya Budhi Udrayana4, Sri Wahjuningsih2*

...motility, viability, and plasma membrane integrity were significantly higher (P<0.01) when 2-3 mM BHT was added, while acrosome integrity was elevated after the addition of 1 and 3 mM BHT. Furthermore, the addition of BHT did not significantly change semen pH. DNA damage was lower (P<0.05) when 3 mM BHT was added and mitochondrial activity was enhanced under the same condition. The concentration of malondialdehyde (MDA) was lower (P=0.05) with the additi...

Afrasyab Khan1,3, Ali Raza Jahejo1,3, Meng-li Qiao1,3, Xin-yu Han1,3, Raza Ali Mangi1,3, Ding Zhang1,3, Yu-hai Bi2, George F Gao1,3* and Wen-xia Tian1,3*

...ext-align: justify;">Mycoplasma synoviae is one of the most important pathogens in the poultry industry and often causes diseases of a chronic and persistent nature. However, there is limited data available on Nuclear factor kappa-light-chain-enhancer of activated B cells (NF-κB) signaling pathway genes expression in chicken erythrocytes infected with Mycoplasma synoviae (M. synoviae). Therefore, the aim of the current...

Rehana Shahida1, Shagufta Naz1*, Tasnim Farasat1, Saima Sharif1, Shah Jahan2 and Farkhanda Manzoor1

...erogenic parameters like plasma glucose level, serum levels of insulin, thrombomodulin and IL-6 were measured by ELISA. The serum level of thrombomodulin was assessed as a measure of vascular damage. This study showed significantly elevated serum concentration of IL-6 and altered lipid profile in diabetes individual when compared with the control subjects. IL-6 and thrombomodulin was progressively higher in diabetic group. IL-6 correlated positively with throm...

Eman Said El-Hadad*, Hesham Ahmed Madian, Mahmoud A.E. Hassan, Mohamed Fahmy Saad, Abdelghany M. El-Shhat, Entesar Zakaria Eliraqy

...study. Hematological and plasma biochemical parameters, liver and kidney function, and antioxidant markers were determined. Results showed that RBCs and lymphocyte increased, while neutrophils and Neut./Lymph ratio decreased in G2, however, Hb, PCV, RBCs, MCH, and lymphocyte increased, while WBCs, neutrophils, and Neut./Lymph ratio decreased in G3 in comparison with G1. Plasma total protein and albumin increased, while total...

Xin-yu Han1,2, Afrasyab Khan1,2, Ali Raza Jahejo1,2, Qian-qing Cheng1,2, Meng-li Qiao1,2, Raza Ali Mangi1,2, Muhammad Farhan Qadir1,2, Ding Zhang1,2, Ying Wang1,2, Yu-hai Bi1,3, Rui-wen Fan1,2 and Wen-xia Tian1,2

...cytes in response to Mycoplasma synoviae. The purpose of the current in-vitro study was to determine the chicken erythrocytes interaction with M. synoviae using a transmission electron microscope (TEM). The mRNA gene expression of TLR1, 2, 3, 4, 5, 7, 15, MHC I, II, and MyD88 in M. synoviae infected chicken erythrocytes was determined using quantitative real-time PCR (qRT-PCR) at four different time intervals (0, 2, 6 and 10 h) post-infection and compared to u...

Babi Kyi Soe1*, Toe Win Naing2, Su Lai Yee Mon1, Nay Chi Nway3, Hiroshi Sato4

...gether on most farms. Anaplasma spp. are obligate intracellular rickettsial vector-borne pathogens that impact on livestock farmers with major economic constraints and eventually threaten human health in cases of zoonotic species involvement. Therefore, our study aimed to identify Anaplasma spp. infection in a bovine host using both microscopy and molecular techniques. A total of 59 samples were collected from Mingaladon tow...

Genlong Zhan1*, Yunhua Zheng2, Desheng Li*4 and Hongmei Zhang3

...ood low shear viscosity, plasma viscosity, as well as erythrocyte aggregation index of both groups decreased after treatment compared with the pre-treatment baseline. The hemorheological indexes of the Yiqi Huoxue Tongmai decoction group were lower than those of the control group. To conclude Yiqi Huoxue Tongmai Decoction has significant clinical effect in the treatment of DPN, can effectively improve the blood rheology of patients, and has the advantages of s...

KAFEEL AHMAD1, NIMRA ARSHAD1, ZAFAR IQBAL KHAN1, KINZA WAJID1, HUMAYUN BASHIR1, IJAZ RASOOL NOORKA2, MAHPARA SHEHZADI3, HAFSA MEMONA4, MADIHA SANA4, KHALID NAWAZ5, MUDASRA MUNIR1, IFRA SALEEM MALIK1, TASAWAR ABBAS6, ABRAR HUSSAIN7, MUHAMMAD SHER8, MUHAMMAD FAHAD ULLAH6, MUHAMMAD AKRAM QAZI9, MUHAMMAD NADEEM10, MUHAMMAD RIZWAN QURESHI10

ZAHID FAROOQ1, IRFAN BABOO2, MUHAMMAD WAJID3, HAFIZA SADIA4, MUHAM

...fferent (p<0.05). The plasma chemical values including the Urea, Alanine aminotransferase, Aspartate aminotransferase, Creatinine, Total Protein and Albumin were determined. The overall biochemical blood values of Urea 282.06±37.18mg/dL, ALT 13.61±2.597 μL, AST 48.23±28.157 μL, Creatinine 61.04±12.658 mg/dL, Total protein 9.29±1.228 mg/dL and Albumin 2.44±0.108 mg/dL and were recorded. All these parameters bet...

Sri Wahjuningsih1*, Ani Atul Arif1, Khaerudin2, Herlina Pratiwi3, Ardyah Ramadhina Irsanti Putri1

...ded motility, viability, plasma membrane integrity, and acrosome integrity. All data obtained were analyzed with two way-analysis of variance. The result showed that equilibration time treatment and differential temperature of thawing yield good results on the motility and viability of Gaga chicken sperm on all treatments (P>0,01). Equilibration time of treatment for 2 hours was capable of preserving Plasma Membrane Integ...

Gokarna Gautam1*, Sajan Rokaya1, Bhuminand Devkota1, Saroj Sapkota2

...basal level (<1ng/ml) plasma progesterone concentration. In conclusion, there was a clear seasonality pattern of reproduction in buffaloes in subtropical regions of southern Nepal with the autumn being the good and the spring being the low breeding as well as calving seasons; and 53% of buffaloes during low breeding season were in true anestrous condition.
 
Keywords | Buffalo, Reproduction, Seasonality, ELISA, Progesterone, Ane...

Zuhair Dardona1,2, Adnan Al-Hindi3, Mohamed Hafidi1, Ali Boumezzough1 and Samia Boussaa1,4*

...ion and is caused by Toxoplasma gondii, an obligate intracellular parasite. The goal of this research is to detect the existence and genotypes of T. gondii in beef and mutton, two of the most widely consumed red meats in Gaza, Palestine, using both ELISA and PCR techniques. For this purpose, 60 red meat samples were collected from butcheries in Gaza city, during the period from January to March 2021. These samples were divided evenly between beef and mutton. T...

Ping Sheng Ye1, Lin Li2, Yu Long Wu3 and Yuan Shu Zhang1*

...nt of milk compositions; plasma samples were collected to measure the content of IGF-1 and GH. The liver and mammary gland samples were collected to observe the expression of IGF-1R and GH. The results showed that compared with the control group, the content of plasma GH and IGF-1 were decreased in the high-concentrate group, and the percentage of lactose and milk yield were also decreased. Meantime, the mRNA expression of I...

Noura K. Al-Suwailem1, Nancy N. Kamel2, Ahmed O. Abbas1,3*, Farid S. Nassar1,3 , Dalia A.A. Elsayed4, Gouda F. Gouda1,5, Hosam M. Safaa6

...l assessment, as well as plasma was employed to measure blood metabolites and antioxidant markers. The results revealed a significant (P<0.05) reduction in red blood cell count (RBC), hemoglobin (HBG) concentration, hematocrit (HCT) percentage, albumin level, and endogenous antioxidant activity. Meanwhile, plasma total protein, cholesterol, triglycerides, creatinine, urea levels, and liver enzymes activity were significan...

Afrah Adil Hassan1*, Hawraa F. H. Alowaid1, Majdy Faisal Majeed2 

...a;) were measured in the plasma samples, and an evaluation of the liver histological profile was conducted on liver samples. The results indicated that lead toxicity significantly (p≤0.05) increased MDA and MOP levels while decreasing GSH activity in the liver compared to the control group. However, this effect was significantly (p≤0.05) altered by the supplementation of Chy and Gnk. Similarly, the levels of TNF-α and IL-1β were significantly ...

Xiaodong Lin*, Guangyao Wu and Fu Zhang

... in epithelial cells and plasma cells in the lamina propria, and its expression level in the observation group was significantly lower than that in the control group (P<0.05). It was concluded that the UC destroys the intestinal mechanical barrier and immune barrier resulting in the dysfunction of intestinal mucosa barrier.

...

Kingsley O. Omeje1, Juliet N. Ozioko2, Amaechi L. Ogara2, Benjamin O. Ezema2* and Dilibe C. Urama3

...here was increase in the plasma concentration of high-density lipoprotein and triacylglycerides, with a decrease in the plasma concentration of low-density lipoprotein, with non-significant increase (p>0.05) in the activities of antioxidant enzymes. The extract did not show alteration in the liver function. Hence, prolonged consumption of the extract has not shown any sign of toxicity and may not predispose the animal to ...
Zakaullah
...ed by a bacterium, Mycoplasma-Like Bodies and aphids respectively were recorded. Bacterial canker was more common and damaging as compared with witche's-broom and aphids attack in Phander areas situated about 200 km north-west of Gilgit at an elevation ranging between 2286 and 2438 m. The important forest trees include willows and poplars. Three species of willows, i.e. Salix purpurea, S. seriocarpa, S. tetrasperma and a single species of poplar...
Naveed Hussain1*, Muhammad Arif Khan1, Asim Khalid Mahmood2, Muhammad Yasin Tipu3, Mamoona Chaudhry4, Hamad Bin Rashid1, Haroon Akbar5 and Abdul Aziz6

Atikah Nur Baity1, Noni Ashri Maghfiroh1, Syalsa Bella Fitriana1, Kurniawan Dwi Prihantoko2, Dyah Maharani2, Diah Tri Widayati2*

... viability, abnormality, plasma membrane integrity, and DNA fragmentation were observed. The result showed that the sperm DNA fragmentation (%) which was stored for 3 months (6.33±0.38), 6 months (6.84±0.61), and 8 months (6.97±0.36) still in good conditions. Sperm motility, viability, and DNA fragmentation remained unaffected in different storage periods (p<0.05). Based on the result above, it could be concluded that storage period aff...

Husnurrizal Husnurrizal1,2, Hafizuddin Hafizuddin2*, Sri Wahyuni3, Cut Nila Thasmi2, Tongku Nizwan Siregar2,4 

...lso assessed using blood plasma from blood samples. Administration of PGF2α significantly affected spermatozoa concentration (p<0.05) at the 90th minute (P3), increased motility (P<0.05) overall treatment times, enhanced viability (P<0.05) at the 30th and 60th minutes (P1 and P2), and reduced abnormalities (P<0.05) in all treatment groups, but it did not affect testosterone levels (P>0.05). The peak of cAMP concentration occurred at P1, wh...
Langgeng Priyanto1,2*, Herdis2, Santoso2, Pradita Iustitia Sitaresmi2
Tri Puji Priyatno2, Rahma Isartina Anwar2, Desiana Ade Mahari2
Florentina Bety Indah Lupitasari2, Maman Surachman2
I Wayan Angga Darmawan2, Arfan Abrar1 and Arum Setiawan3
...bility, abnormality, and plasma membrane quality of post-thawed Tropical Brahman bull sperm. Sperm samples were obtained and pooled from eight sexually mature Tropical Brahman Bull, separated into seven equal proportions, and diluted with TEY extender Semen was supplemented with 0 (C0;E0), 5 (C5;E5), 10 (C10;E10), and 15 (C15;E15) mg/mL of each α-tocopherol and ascorbic acid, respectively. Total sperm motility, viability, abnormalities, and semen membran...

Saba Mehnaz1*, Farhan Ahmad Atif2, Rao Zahid Abbas1, Muhammad Kasib Khan1 and Muhammad Saqib3

...carried out to detect Anaplasma marginale and Theileria annulata from buffaloes of Sheikhupura, Jhang, and Rawalpindi districts by using duplex PCR. A total of 1,152 blood samples of buffaloes were screened for epidemiological investigations. DNA extracted from blood samples was used for the amplification of the msp1β gene and cytochrome b gene by using single and duplex PCR. The prevalence of these pathogens and associated risk factors were observed thro...

Mohammed Abdulameer Rashid Al-Sarray1, Hasanain Jihad Neamah2*, Sarah Gatea Fayyadh AL-Omairi1

...imate the TAC of seminal plasma depending on the absorbance. The P1, P2, O1, and O2 groups recorded a highly significant (P≤0.01) decrease in seminal MDA level. The P1, P2, O1, and O2 groups recorded a highly significant (P≤0.01) decrease in seminal MDA level. On the other hand, the results suggested a higher TAC increase for all groups than the control at different times of cooling and post-cryopreservation. In conclusion, adding 2 mg of O.M. powder ext...
Ilker Ugulu1*, Zafar Iqbal Khan2, Kafeel Ahmad2, Humayun Bashir2
Naunain Mehmood3, Asma Zafar2 and Muhammad Sohail2
...n soil, forage and blood plasma of horses in Central Punjab, Pakistan. Concentrations of metals, cobalt (Co), selenium (Se), manganese (Mn) and iron (Fe) in these samples were determined. Atomic Absorption Spectrophotometer was used to determine the concentration of metals. The concentrations of all the analysed heavy metals differed significantly with different seasonal periods. The mean Fe, Co, Mn and Se values in blood plasma...

Sumaya Loay Mohamed Shams Al-Dean1*, Sura Shakir Hammoud2, Mohammedali J. Ghafil3

... motion characteristics, plasma membrane integrity, and normal acrosome morphology were evaluated using paired sample T-test. The p-value (<0.05) is considered statistically significant. After thawing, the sperm cells’ motility dramatically increased with the addition of 2 mM (56.8±1.16), 4 mM (57.6±1.36), and 7 mM (62.1±1.67). The sperms viability is improved post-thawimg and at 5C after addition of anti-oxidants vitamin C. When ...

Dwi Walid Retnawati1,2, Mohamad Agus Setiadi3*, Iman Supriatna3, Ligaya Ita Tumbelaka3

... preliminary research of plasma MDA levels in 20 cows comprising 5 RP and 15 non-placental retention (NRP). This was followed by the determination of Se and MDA levels in 46 cows consisting of 21 RP and 25 NRP. The samples were obtained from clinical conditions of dairy cows in the local community in Indonesia which have the recording of RP. The blood samples were taken by 4 groups at different times such as at 3 weeks and 1 week prepartus, 1 day and 3 weeks p...

Muhammad Tahir1, Muhammad Saqib1, Shahbaz ul Haq2, Shahrood Ahmed Siddiqui3,4, Khurram Ashfaq1, Urfa Bin Tahir5, Mughees Aizaz Alvi1*, Shujaat Hussain6, Talha Javaid1, Raheela Taj7, Muneeb Islam8, Imad Khan9, Asad Ullah9* and Shakirullah Khan10

... population as well. Toxoplasma gondii is transmitted to ruminants through ingestion of oocytes-contaminated herbage. The purpose of the current study was to report prevalence and access the risk factors associated with toxoplasmosis. A total of 652 specimens of blood were taken from 9 regions situated within the jurisdiction of Punjab province, Pakistan. The collected samples were labeled in order to have randomization and to pull 384 samples out of 652 harve...

Wirokartiko Satyawardana1,4, Umi Cahyaningsih2*, Fadjar Satrija2, Safika Safika3, Arifin Budiman Nugraha2

...li, Hepatozoon canis, Anaplasma platys, and Ehrlichia canis. Using the t-test, data analysis, which covered numerous categories, comprising city of origin, sex, and age for each species of blood parasites and rickettsia, showed that only the male had a significant difference in Babesia canis vogeli infections with P-value of 0.023. In contrast to the other categories, there were no significant differences. Based on the construction of a phylogenetic tree of Ne...

Qamar Ullah1,2, Muti-ur-Rehman Khan1*, Raheela Akhtar1 and Aftab Ahmad Anjum3

...ups, i.e., platelet-rich plasma (PRP) group (n=32), antibiotic group (n=32) and PRP plus antibiotic group (n=32). Whole blood was taken from the animal’s jugular vein, and PRP was prepared by double spin open method and infused intra-mammary to the same animal from which the blood was taken. An intra-mammary antibiotic was administered intra-mammary to the animals of the antibiotic group. PRP plus antibiotic was allocated to the animals of the combined a...

Walid Elmonir1*, Ahmed Abdel-Fattah Tayel2, Suzan Abdelbaky Kotb1 and Wael Fawzy El-Tras2

... fish as a source of Toxoplasma gondii and to determine effective decontamination approaches. A total of 200 agricultural produce samples (50 per each of carrots, radishes, lettuces, and strawberries) and 315 fish samples (200 Mullet and 115 Tilapia) were collected. The T. gondii were identified in these samples by microscopy and molecular analysis. In total, 9% (18/200) of agricultural produce samples had Toxoplasma oocysts...

Pakistan Journal of Zoology

October

Pakistan J. Zool., Vol. 56, Iss. 5, pp. 2001-2500

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