Polymorphism Analysis of GHRL Gene in Goat
Polymorphism Analysis of GHRL Gene in Goat
Jun Yan Bai1,*, Yong Gang Zhao2 and Yu Qin Wang1
ABSTRACT
This study is to search candidate genes that control goat growth and explore SNP loci of GHRL genes, thus laying foundations for genetic marker of growth traits of goats. In this study, GHRL genes of black goat and Yaoshan goat were collected for PCR amplification. Polymorphism of goat species was tested by sequencing technology. Results demonstrated that there are two mutation sites (SNP loci) of the primer GHRL-2 in black goat and Yaoshan goat, which are G447C site and T498C site. At G447C site, gene frequencies of G and C in black goat and Yaoshan goat were detected 0.621/0.379 and 0.793/0.207, respectively. In black goat and Yaoshan goat, G is the dominant allele in G447C. At T498C site, gene frequencies of T and C in black goat and Yaoshan goat were tested 0.675/0.325 and 0.847/0.153, respectively. At this site, T is the dominant allele in Yaoshan goat. In black goat, GHRL-6 has polymorphism and its mutation site is C105T. The gene frequencies of C and T at C105T in black goat were detected 0.698 and 0.302, respectively. T is the dominant allele at C105T in black goat. These research results lay a theoretical foundation for further correlation analysis with growth indexes of goats.
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February 2019
Vol. 51, Iss. 1, Pages 1-398
