Sequence Analysis of SAG2 of Feline Toxoplasma gondii Oocysts in Pakistan

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Sequence Analysis of SAG2 of Feline Toxoplasma gondii Oocysts in Pakistan

**Habibun Nabi¹, Saher Islam², Amna Arshad Bajwa², Imran Rashid^1,*, Haroon Akbar¹, Wasim Shehzad², Kamran Ashraf¹, Nisar Ahmad¹ and Aneela Durrani³**

Author Affiliations

¹Molecular Parasitology Laboratory Department of Parasitology, University of Veterinary and Animal Sciences, Lahore, Pakistan

²Institute of Biochemistry and Biotechnology, University of Veterinary and Animal Sciences, Lahore, Pakistan

³Department of Clinical Medicine and Surgery, University of Veterinary and Animal Sciences, Lahore, Pakistan

* Corresponding author: [email protected]

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Fig 1

Agarose gel eletrophoresis of SAG2 gene products (242, 171, 211, 230 and 288bp).

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Fig 2

Phylogenetic and bootstrapping analysis of 3 isolates with published archetypal lineages and atypical strains. UVAS-Toxo 1, 3 and 6 are our isolates from cat feces. XM_00384580 is the SAG2 sequence of Neospora caninum served as out-group. AF357581 is the SAG2 sequence of atypical T. gondii strain.

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Fig 3

Clustal W alignment of 3 isolates of this study with the representative 3 clonal strains of Type I (EU053942), Type II (EU053943) and Type III (EU053944). Red rectangular marked sequences show dissimilarity of our isolates at 5’ locus of SAG2 with Type I, II and III strains.

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December 2017

Vol. 49, Iss. 6, Pages 1937-2341

Pakistan Journal of Zoology

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Sequence Analysis of SAG2 of Feline Toxoplasma gondii Oocysts in Pakistan

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Sequence Analysis of SAG2 of Feline Toxoplasma gondii Oocysts in Pakistan

**Habibun Nabi¹, Saher Islam², Amna Arshad Bajwa², Imran Rashid^1,*, Haroon Akbar¹, Wasim Shehzad², Kamran Ashraf¹, Nisar Ahmad¹ and Aneela Durrani³**

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Sequence Analysis of SAG2 of Feline Toxoplasma gondii Oocysts in Pakistan

Sequence Analysis of SAG2 of Feline Toxoplasma gondii Oocysts in Pakistan

Habibun Nabi1, Saher Islam2, Amna Arshad Bajwa2, Imran Rashid1,*, Haroon Akbar1, Wasim Shehzad2, Kamran Ashraf1, Nisar Ahmad1 and Aneela Durrani3

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**Habibun Nabi¹, Saher Islam², Amna Arshad Bajwa², Imran Rashid^1,*, Haroon Akbar¹, Wasim Shehzad², Kamran Ashraf¹, Nisar Ahmad¹ and Aneela Durrani³**