Sequencing and Phylogenetic Analysis of P4b Gene in Pigeon Poxvirus
Sequencing and Phylogenetic Analysis of P4b Gene in Pigeon Poxvirus
Rawaa Saladdin Jumaa
The agarose gel electrophoresis pattern demonstrated the conventional PCR for the P4b gene in infected birds (M=leader, lane1-10 samples).
(A) Non-pathogenic CAM (Control); (B) Infected CAM with thickened and hemorrhagic at 1st passage; (C), (D) Infected CAM with Pock lesions at 2nd and 3rd passages.
Cytopathic effect of the virus when propagated in embryonated chicken eggs; (A) Uninfected cell culture (control); (B) cell rounding, aggregation and sloughing of it at 1st passage after 48h; (C) and (D) cell aggregation and sloughing after 24h and 48h of 2nd passage.
Three dimensional models of P4b gene for pigeon poxvirus (A), Model evaluation (Ramachandran plot) of P4b gene for pigeon poxvirus (B), Model validation (local estimation and Z score) of P4b gene for pigeon poxvirus (C and D).
Phylogenic tree of Iraqi sample that submitted to NCBI under the accession number (MT499377.1, MT499380.1, MT499381.1, MT499376.1, MT499372.1, MT499373.1, MT499378.1, MT499374.1, MT499375.1 and MT499379.1) using Mega 6 software for P4b.
