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Synthetic Oviductal Fluid Medium with Isologous Oviductal Epithelial Cells Improves the Development of In Vitro Produced Nili Ravi Buffalo (Bubalus bubalis) Embryos

Synthetic Oviductal Fluid Medium with Isologous Oviductal Epithelial Cells Improves the Development of In Vitro Produced Nili Ravi Buffalo (Bubalus bubalis) Embryos

Asima Azam1, Asma-Ul-Husna2, Saima Qadeer3, Qaisar Shahzad4, Rabea Ejaz1, Nemat Ullah5, Tasneem Akhtar4 and Shamim Akhter2,*

1Department of Zoology, Shaheed Benazir Bhutto Women University, Peshawar 25000
2Animal Physiology Laboratory, Department of Zoology, PMAS-Arid Agriculture University, Rawalpindi 46300
3Department of Zoology, University of Education, Jauharabad Campus, Jauharabad-41200
4Buffalo Research Institute Pattoki, Kasur, Lahore 54000
5Department of Animal Reproduction, Riphah International University, Lahore 54000

*      Corresponding author: shamim@uaar.edu.pk.com

 

Fig. 1.

Cumulus oocyte complexes (COCs) before (A) and after (B) 24 h of maturation.

Fig. 2.

Assessment criteria for developmental stages.

Fig. 3.

Percentage of morula per cleaved zygotes in different IVC treatments (Group I, SOF alone; Group II, SOF+Co-culture; Group III, Conditioned SOF; Group IV, M199 alone; Group V, M199+Co-culture; Group VI, Conditioned M199).

Pakistan Journal of Zoology

October

Pakistan J. Zool., Vol. 56, Iss. 5, pp. 2001-2500

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