Synthetic Oviductal Fluid Medium with Isologous Oviductal Epithelial Cells Improves the Development of In Vitro Produced Nili Ravi Buffalo (Bubalus bubalis) Embryos

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Synthetic Oviductal Fluid Medium with Isologous Oviductal Epithelial Cells Improves the Development of In Vitro Produced Nili Ravi Buffalo (Bubalus bubalis) Embryos

Asima Azam¹, Asma-Ul-Husna², Saima Qadeer³, Qaisar Shahzad⁴, Rabea Ejaz¹, Nemat Ullah⁵, Tasneem Akhtar⁴ and Shamim Akhter^2,*

Author Affiliations

¹Department of Zoology, Shaheed Benazir Bhutto Women University, Peshawar 25000

²Animal Physiology Laboratory, Department of Zoology, PMAS-Arid Agriculture University, Rawalpindi 46300

³Department of Zoology, University of Education, Jauharabad Campus, Jauharabad-41200

⁴Buffalo Research Institute Pattoki, Kasur, Lahore 54000

⁵Department of Animal Reproduction, Riphah International University, Lahore 54000

* Corresponding author: [email protected]

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Fig. 1.

Cumulus oocyte complexes (COCs) before (A) and after (B) 24 h of maturation.

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Fig. 2.

Assessment criteria for developmental stages.

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Fig. 3.

Percentage of morula per cleaved zygotes in different IVC treatments (Group I, SOF alone; Group II, SOF+Co-culture; Group III, Conditioned SOF; Group IV, M199 alone; Group V, M199+Co-culture; Group VI, Conditioned M199).

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December 2019

Vol. 51, Iss. 6, Pages 1999-2399

Pakistan Journal of Zoology

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Synthetic Oviductal Fluid Medium with Isologous Oviductal Epithelial Cells Improves the Development of In Vitro Produced Nili Ravi Buffalo (Bubalus bubalis) Embryos

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Synthetic Oviductal Fluid Medium with Isologous Oviductal Epithelial Cells Improves the Development of In Vitro Produced Nili Ravi Buffalo (Bubalus bubalis) Embryos

Asima Azam¹, Asma-Ul-Husna², Saima Qadeer³, Qaisar Shahzad⁴, Rabea Ejaz¹, Nemat Ullah⁵, Tasneem Akhtar⁴ and Shamim Akhter^2,*

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Synthetic Oviductal Fluid Medium with Isologous Oviductal Epithelial Cells Improves the Development of In Vitro Produced Nili Ravi Buffalo (Bubalus bubalis) Embryos

Synthetic Oviductal Fluid Medium with Isologous Oviductal Epithelial Cells Improves the Development of In Vitro Produced Nili Ravi Buffalo (Bubalus bubalis) Embryos

Asima Azam1, Asma-Ul-Husna2, Saima Qadeer3, Qaisar Shahzad4, Rabea Ejaz1, Nemat Ullah5, Tasneem Akhtar4 and Shamim Akhter2,*

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Asima Azam¹, Asma-Ul-Husna², Saima Qadeer³, Qaisar Shahzad⁴, Rabea Ejaz¹, Nemat Ullah⁵, Tasneem Akhtar⁴ and Shamim Akhter^2,*