The Effects of Equilibration Time and Post-Thawing Temperatures in Cryopreservation of Gaga Chicken Semen
Sri Wahjuningsih1*, Ani Atul Arif1, Khaerudin2, Herlina Pratiwi3, Ardyah Ramadhina Irsanti Putri1
1Department of Animal Reproduction and Genetic, Faculty of Animal Science, University of Brawijaya, Jl. Veteran, Malang 65145, East Java, Indonesia; 2Student of Doctoral program in Animal Science, Faculty of Animal Science, University of Brawijaya, Jl. Veteran, Malang 65145, East Java, Indonesia; 3Department of Veterinary Medicine, Faculty of Veterinary Medicine, University of Brawijaya Jl. Puncak Dieng, Malang 65151, Indonesia
*Correspondence | Sri Wahjuningsih, Department of Animal Reproduction and Genetic, Faculty of Animal Science, University of Brawijaya, Jl. Veteran, Malang 65145, East Java, Indonesia; Email: yuning@ub.ac.id
Figure 1:
Viability of sperm Gaga chicken a. dead sperm b. live sperm.
Figure 2:
Plasma membrane integrity of sperm a. damaged PMI b. intact PMI (swelling in tail of sperm).
Figure 3:
Acrosome Integrity of sperm Gaga chicken using colouring solution of Coomassie Brilliant Blue (CBB) a: integrated acrosome (blue coloured in head sperm) b: unintegrated acrosome (less coloured in head sperm).