A Simplified Medium for In Vitro Digestion of Ruminants: Cattle-Fed Rice Straw with Supplementation of Legume Foliage
A Simplified Medium for In Vitro Digestion of Ruminants: Cattle-Fed Rice Straw with Supplementation of Legume Foliage
Danh Mo1*, Nguyen Van Thu2
ABSTRACT
An experiment was carried out to evaluate the effect of a supplement of Sesbania grandiflora L. foliage (SF) on the nutrient digestibility of crossbred (local × Red Sindhi) cattle fed basal diets of rice straw (RS) in Southwestern Vietnam. The experiment was designed to change each of the 4 cattle fed 6 diets over 6 periods, the dietary treatments were RS with the supplementation of SF from 0, 7, 14, 21, and 28 to 35% of total dry matter (DM) intake. Urea was also used to adjust the crude protein content in diets to approximately 11% of DM. The dietary organic matter digestibility (OMD) and metabolizable energy (ME) were determined by the in vivo, formal in vitro (F_iv) and in vitro procedure of simplified medium (S_iv) by eliminating trypticase, minerals and reducing solutions and replacing them with rumen fluid from slaughterhouses of cattle with unknown dietary histories. The results showed that the dietary OMD, total digestible nutrients, and ME values significantly (P<0.05) increased according to the rise of the SF supplement. Still, the digestible fiber had a significant (P<0.05) decreasing trend. The OMD and ME values (n=24) estimated by the S_iv procedure had a close linear relationship to the in vivo (R2 0.78 - 0.80) and F_iv (R2 0.86 - 0.87). It is, therefore, concluded that the in vitro test of digestion could utilize rumen fluid from slaughterhouses and eliminate some chemicals in the medium to satisfy better concerns associated with animal ethics and welfare, environment and cost.
Keywords | Digestibility, In vivo, In vitro, Medium, Rumen fluid, Sesbania
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