CDX1 Gene Expression in Recurrent Spontaneous Abortion under Extracellular Regulatory Protein Kinase1/2 Signaling Pathway
CDX1 Gene Expression in Recurrent Spontaneous Abortion under Extracellular Regulatory Protein Kinase1/2 Signaling Pathway
Shouyan Cao1, Aili Yan2, Wenhua Zhang3, Fangfang Li1 and Xiaoning Liu4*
ABSTRACT
The aim was to explore the mechanism of the gene expression of caudal-related homeobox transcription factor 1 (CDX1) in recurrent spontaneous abortion (RSA) based on the extracellular regulatory protein kinase 1/2(ERK1/2) signaling pathway. In this study, 25 non-pregnant female patients were grouped into the experimental group (group B), who were admitted to the Binzhou Medical University Hospital from August 15, 2022 to June 10, 2023. Besides, 25 non-pregnant healthy female employees were selected as the control group (group A). The DNA was extracted from venous blood, which was amplified by polymerase chain reaction (PCR). Besides, the uterine tissue samples were processed and subjected to immunohistochemistry to monitor the phosphorylation level of ERK1/2, so as to analyze the expression of decidua and villous tissue cells in cytoplasm from group A and B. The results showed that the decidua and villous tissue cells of patients with RSA were almost not expressed in the cytoplasm. Through the χ2 test, the frequency of CC genotype in patients from group B was sharply lower than that of group A (χ2=13.9 and P<0.05). However, the frequency of TT genotype in patients from group B was markedly higher than that of group A (χ2=9.1 and P<0.05), and the frequency of TT genotype in patients with 3 or more than 3 abortions was higher than that of patients with less than3 abortions. Therefore, it proved that there was a damage in the ERK1/2 signaling pathway in the cytoplasm of decidua and villous tissue cells of patients with RSA. RSA was also closely related to homocysteine, folic acid and vitamin B12 levels in serum. Moreover, the level of homocysteine in serum is associated with mutations of CDX1.In addition, patients with more than 3 abortions indicated a greater probability of CDX1 abnormalities.
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