Commercial Fumigant Fitness and Bio-Pesticides Potential against Resistant Strains of Quarantine Insect Pests

Abdul Khaliq1, Muhammad Irfan Ullah1,*, Muhammad Afzal1, Akhlaq Ahmad2 and Yasir Iftikhar3

1Department of Entomology, University of Sargodha, Sargodha 40100

2Food Quality and Safety Research Institute, Southern-zone Agricultural Research Centre, Pakistan Agricultural Research Council, Karachi University Campus, Karachi

3Department of Plant Pathology, University of Sargodha, Sargodha 40100

ABSTRACT

Food spoilage of perishable and other grains are seriously proliferated during storage by stored grain insect pests under variable conditions. Resistance development in insect pests against fumigants is a serious threat to effective commercial use of fumigants in various quarantine treatments. Therefore, resistant strains of Tribolium castaneum (Herbst), Rhizopertha dominica (Fabricius) and Sitophilus oryzae (Linnaeus) were tested against phosphine and bio fumigants to check their fitness and potential following by F1 generation. Phosphine fitness in term of mortality mean calculated at 300 ppm was minimum against weevil but highly effective against red flour beetle after 24hours while, at 400ppm showed same resistance level in all. At 500ppm, lesser grain borer (LGB) was more susceptible as compared to others as in rice weevil. Nicotiana tabacum (L.) and Calotropis procera (Aiton) oil both at 15% were most lethal to LGB, weevil and red flour beetle after 72hours following by effect of Datura stramonium (L.) and Eucalyptus camaldulensis (Dehnh) with respect to control. Similar response was noted at 10% concentrations of all plants but E. Camaldulensis (5%) was least effective against all insects after control. Resurgence response in F1 generation in each experiment showed high multiplication rate at low concentrations and vice-versa also suppressed against N. tabacum and C. procera. Hence, developing PH3 resistance can be managed with bio-pesticides up to extant and need more work to make it applicable in field.

Article Information

Received 14 June 2017

Revised 23 July 2018

Accepted 24 December 2018

Available online 15 April 2019

Authors’ Contribution

AK and MIU conceived, designed and performed the experiments. MA and AA interpreted the data and wrote the paper. YI reviewed and edited the manuscript.

Key words

Phosphine, Biopesticides, Tribolium castaneum, Rhizopertha dominica, Sitophilus oryzae.

DOI: http://dx.doi.org/10.17582/journal.pjz/2019.51.3.1075.1084

* Corresponding author: mirfanullah@uos.edu.pk

0030-9923/2019/0003-1075 $ 9.00/0

Copyright 2019 Zoological Society of Pakistan

Introduction

Food commodities like food grains are stored all over the world as a major component of the food security till the next season harvest (Daglish et al., 2015). Efficiency of the adopted prophylactic mitigations is highly flexible and directly proportional to the length of the storage (Benhalima et al., 2004). In response to long time storage lead from mild to severe qualitative and quantitative losses due to proliferation by plenty of stored grain insect pests (Nenaah, 2014). Direct consumption not only effects the germination but also reduces the nutrients level as well as insect products like webbing, exuviae, body fragments, silk or other chemical secretions, dead and live insects may unfit food for human consumption (Nenaah, 2014; Rajendran and Sriranjini, 2008; Serin, 2016).

Among stored grain insect pests three insect pests as T. castaneum, red flour beetle (RFB), R. dominica, the lesser grain borer (LGB) and S. oryzae, the rice weevil (RW) are documented as notorious polyphagous stored grain insect pests that has got resistance against commercial fumigants and is a serious threat for plenty of cereal crops including wheat (Ashouri and Shayesteh, 2009; Bughio and Wilkins, 2004; Daglish et al., 2014; Lira et al., 2015; Liu et al., 2007; Nenaah, 2014). It is estimated that about 90% wheat grains or its products are being used for the human consumption where insect losses have been reported 9% in developed countries while more than 50% are recorded in the under developed (Brader et al., 2002; Kwiatkowska et al., 2014). Stored grain insect pests cause 20-30% losses in the tropical and sub-tropical region while 5-10% losses have been reported in the temperate zone (Nenaah, 2014; Rajendran and Sriranjini, 2008).

Wheat losses due to insect pests in Canada and United States of America were noted nearly about 20-26% while in Asia 6.5% losses were recorded in India (Pant et al., 2014). Around the globe post-harvest losses due to pest infestation may accede from 10-40%. For 6-8 month storage duration of the small land holding farmers, the losses can be very high as much as 80% (Ogendo et al., 2008; Pant et al., 2014). Among other cereals, in a season all over the world maize production is infested by rice weevil cause losses 1-2% developed world and 14-50% in under develop countries (Suleiman et al., 2015). In Pakistan, 2-6% food grains of the total grains produced are infested by store product pests during storage annually (Saleem et al., 2014).

Managing these insect pests with plenty of chemical insecticides employed like magnesium phosphide, aluminum phosphide, Methyl bromide, Deltamethrin, Pyrethroids, organophosphates and primiphos-methyl raised plentiful problems like food toxicity, deterioration, adulteration and contamination, insect resistance, environmental effects and lethal for the beneficial insect fauna (Chen and Chen, 2013; Eissa et al., 2014; Fouad et al., 2014; Nenaah, 2014).

Therefore, phosphine resistant strains of T. castaneum, R. dominica and S. oryzae are reported in different areas of the world which have adopted themselves to neutralize the phosphine effect like by changing respiration rate or chemistry of active compounds or going to inactive stage (Bughio and Wilkins, 2004; Sousa et al., 2009; Tapondjou et al., 2002). Two distinct levels for inheritance of phosphine resistance were diagnosed in red flour beetle where rph1 and rph2 genes were responsible for week and strong resistance, respectively (Bengston et al., 1999; Daglish et al., 2015). Furthermore, homozygous beetles carried gene rph1 showed week resistance while, homozygous beetle insects possessed both genes showed strong resistance as genes were incompletely recessive and not sex linked (Bengston et al., 1999). In eastern Australia a study conducted in 1980s sowed a little phosphine resistance development in field population of stored grain insect pest. Twenty two Brazilian populations of Sitophilus weevils were tested for phosphine resistance under FAO standard methods, 20 of which had inherited resistant (Pimentel et al., 2009). In Morocco, 32% samples of stored grain insect pests population collected from various storage facilities were found having a significant number of phosphine resistant populations (Benhalima et al., 2004).

Despite heavy dependence on phosphine and methyl bromide alternative strategies against massive use of these commercial fumigants need to adapt from in-vitro to in-vivo like use of plant extract, essential oils, leaf powders, seed oils, pathogenic fungi and other inorganic means like ozone, carbon dioxide and temperature (Betancur et al., 2010; Rajendran and Sriranjini, 2008; Zandi-Sohani and Ramezani, 2015). Owing to several modes of action plant oils designated as potential fumigant of contact toxin, antifeedant, repellent and disruptors of cuticle as well as they are supposed good to suppress insect growth and fecundity significantly (Daglish and Pulvirenti, 1998; Zandi-Sohani and Ramezani, 2015). Plenty of biopesticides significantly influence the behavioral and physiological response of stored product pests like lethal, growth regulatory, repulsive and reduce reproduction (Hanif et al., 2015). Plant derived insecticides in the form of plant oils, extract, ash, leaf powders, seed oils and lectins has been purposed as possible part of integrated pest management (IPM) of stored grain insect pests (Demissi et al., 2003; Lira et al., 2015). However, profuse studies showed N. tabacum, C. procera, D. stramonium, A. indica and E. camaldulensis are the examples of promising phytochemical plants that possess potential to control the stored grain beetles (Gusmao et al., 2013; Hanif et al., 2015; Hasan et al., 2014; Jemâa et al., 2013; Sagheer et al., 2013; Pant et al., 2014; Saleem et al., 2014). Medicinal plant belong to family Meliaceae, Solanaceae, Apiaceae, Laminaceae, Lauraceae and Myrtaceae actively own insecticidal or toxicological properties (cyanohydrins, monoterpenoids, thiocynates, alkaloids and others) as fumigant (Nenaah, 2014; Rajendran and Sriranjini, 2008). N. tabacum at 10% concentration caused 6.69% insect mortality (Sagheer et al., 2013). Similarly among other biopesticides highest mean mortality (14.36%) was recorded for A. indica (Hasan et al., 2012). Studies for comparative efficacy of plant oils were found best against red flour beetle and other stored product insect pests (Gusmao et al., 2013; Hanif et al., 2015; Jemâa et al., 2013).

Bioassays were conducted to check phosphine fumigation prevalence and fitness as well as assessment of toxicological impact of indigenous biopesticides against resistant strains of T. castaneum, R. dominica and S. oryzae under laboratory conditions. Furthermore, to reflect their resurgence level F1 progeny of surviving insects was also checked.

 

Materials and methods

Collection and rearing of test insects

Resistant population strains of T. castaneum, R. dominica, and S. oryzae were collected from grain market, Government godowns and reported flour mills located in Karachi (Longitude 67.02 East; Latitude 24.92 North). After collection, these insect pests population were kept in the separate jars covered with muslin cloths. Insect populations were regularly checked for their growth and were sieved and transfer to new uninfected sterilized wheat grains diet. At laboratory temperature 30±2ºC and relative humidity at 65±5% was maintained for insect’s maximum growth. Homogenous population of equal size and age was sieved out separate for each insect that later on was used for different bioassay studies (Hanif et al., 2013, 2015; Pant et al., 2014; Saleem et al., 2014).

Plant collection

The fresh leaves of Azadirachta indica (L.), Eucalyptus camaldulensis (Dehnh) Myrtaceae, Myrtales), Calotropis procera (Aiton) (Apocynaceae, Gentianales), Datura stramonium (L.) (Solanaceae, Solanales) and Nicotiana tabacum (L.) (Solanaceae, Solanales) were collected from Food Quality and Safety Research Institute, Pakistan Agricultural Research Council, and Karachi University Campus Karachi (Longitude 67.02 East; Latitude 24.92 North) from 15 May to 15 June 2015 from their natural habitats and were identified by taxonomic specialists. Collected leaves were shadow dried under good ventilation and woody stems were separated. Dried samples were kept in separate plastic bags inside a refrigerator until the time for oil extraction (Hanif et al., 2015; Saleem et al., 2014; Ziaee et al., 2014).

Essential oil extraction

Dried leaves were first ground into powder than essential oils were obtained using ethanol by steam distillation method. The ground powder of all collected plant material was run on Soxhlet’s apparatus with ethanol as solvent in the flask. After that solvent was evaporated leaving the essential oil. The obtained essential oils were dried over sodium sulfate (Merck) and were kept in separate vials (volume 2 mL) with aluminum caps inside a refrigerator to be used later (Hanif et al., 2013, 2015; Hasan et al., 2014; Saleem et al., 2014; Ziaee et al., 2014).

Generation of phosphine gas

The phosphine gas was generated by FAO’s method. The apparatus for generation of phosphine gas consisted of a 5 liter beaker, a collection tube (cylinder), an inverted funnel, Phostoxin® (aluminum phosphide) tablets and muslin cloth. The tube for collection of gas was sealed from one side with airtight rubber stopper and then was filled with 5% sulphuric acid (H2SO4) solution. Half of the beaker was also filled by 5% H2SO4 solution.

The gas collecting tube was placed carefully into the beaker over the inverted funnel in such a way that there is no loss of H2SO4 solution from the collection tube, while dipping into the beaker. Before generating phosphine gas all air in collection tube was removed within collection tube. Then phostoxin tablets (wrapped in aluminum foil goes down). When it was filled, 5 ml gas was suck out with the help of an air tight syringe and was injected into a sealed desiccator of known volume then 50 ml of gas was taken out from the desiccators and injected into phosphine meter for measuring gas concentration. With the help of phosphine meter required concentrations of 300 ppm, 400 ppm and 500 ppm of phosphine gas were obtained (Hanif et al., 2013, 2015).

Phosphine fumigation bioassays for prevalence and fitness

Bioassays was followed the methods recommended by FAO method. A phosphine source was generated from an aluminum phosphide tablet and collected over acidified water. The source concentration was measured by gas chromatography using a gas density balance (Aerograph Model 90-P; Varian, Mount Waverley, Victoria, Australia). Adults of homogenous age was added to ventilate plastic soufflé cups which were then placed inside gas-tight desiccators and gas-tight syringes was used to inject the required amount of phosphine through a septum in the lid of each desiccators. Adults were fumigated at 300, 400 and 500 ppm and data was recorded after 24, 48 and 72 h until end-point mortality was assessed. Sterilized food was given to surviving adults in separate jar of each treatment to asses F1 progeny after thirty days (Hanif et al., 2013, 2015).

Screening bioassay for toxicological impact of indigenous bio pesticides against stored grains insect pests

Response of biopesticide oils in mortality bioassay was tested against test under laboratory conditions. For this purpose, different concentrations (5, 10 and 15%) of each botanical were applied on Whatman filter paper (Whatman No.1, qualitative filter paper has the pore size of 11 µm carrying 150mm diameter circles and 460mm x 570mm sheets) placed in having 20 sterilized cereal grains (wheat) for each treatment. Twenty insects for each insect strain were used for each treatment; the control was kept without any insecticide application. Each treatment was replicated three times; each contained about 60g of the wheat grains into each plastic vial (10mL). Whole experiment was performed in the laboratory keeping in an incubator at 30±2°C and 65±% RH. Data regarding mortality was noted at regular time intervals (24, 48 and 72 h). Surviving adults were shifted in separate vials (considering treatments) having sterilized food and data for F1 progeny was recorded after 30 days for the completion of the experiment (Hanif et al., 2013, 2015).

Data recording procedure for F1 progeny development

Surviving adults along with food grains collected from mortality bioassay were shifted in separate vials (considering treatments) having additional sterilized food. Data for adult emergence as F1 progeny was counted in number (adult beetles developed) after 30 days for the completion of the experiment (Hanif et al., 2013, 2015; Mahmoud et al., 2014).

Statistical analysis

Statistical analysis initially, the mortality data were transformed by arcSen in order to normalize the data. Once normalized the data were analyzed by ProGLM to carry out an ANOVA; then the means were separated by Tukey test (p=0.05). For statistical analysis CRD Factorial was used for all collected data of percent mortality and F1 progeny was subject to analysis of variance using Statistica-8 software (Hanif et al., 2013, 2015).

 

Results

Phosphine fitness against insects

Resistance inheritance level in three coleopteran pest strains was significantly (P<0.05) determined. Studies also conducted to evaluate the fitness of quarantine fumigant as well as to measure biocidal potential echelon of some plant oils for their management. Fumigant fitness was less affective at 300 ppm as rice weevil (4.33±0.47SE) showed highest resistance compared to T. castaneum (7.11±0.42SE) after 24 h but there was not abrupt change in mortality after 48 and 72 h. Population of S. oryzae was recorded most challenging among other coleopteran beetles at 500 ppm where insect mortality in R. dominica and T. castaneum was highest (16.00±0.29SE), (15.89±0.56SE) after all time intervals proved to possess weak resistance with respect to control treatment, respectively. Inheritance of phosphine resistance at 400ppm dose was same but averagely low in response showing that weevil has not been considerable affected as compare to red flour beetles and lesser grain borer as shown in Figure 1.

Progeny production

Insect resurgence in term of F1 production/emergence was significantly (P>0.05) checked after 30 days to find the response of tested strains to phosphine toxicity as shown in Table I. Progeny development counted in numbers was high with decrease in phosphine concentrations (500 >400 >300 ppm) against all insects with respect to control (0 ppm) treatment. Over all weevil multiplication rate was high (32.22±1.30SE), (21.67±1.30SE), (11.00±1.30SE) at 300, 400 and 500 ppm with respect to control (51.67±2.25SE) but squat in T. castaneum (7.11±1.05SE), (10.89±1.05SE), (12.89 ± 1.05SE) and R. dominica (21.00 ± 0.74SE), (11.44 ± 0.74SE), (4.44 ± 0.74SE), respectively.

 

Table I.- Concentration based response of commercial fumigant (Means± SE*) on rate of adult emergence (number) with comparisons to control against R. dominica, T. castaneum and S. oryzaeafter thirty days.

Test insects	Treatments	F1 progeny (Means± SE*)
S. oryzae	500ppm	32.22±1.30B
	400ppm	21.67±1.30C
	300ppm	11.00±1.30D
	Control	51.67±2.25A
R. dominica	500ppm	21.00±0.74B
	400ppm	11.44±0.74C
	300ppm	4.44±0.74D
	Control	37.33±1.28A
T. castaneum	500ppm	12.89±1.05A
	400ppm	10.89±1.05B
	300ppm	7.11±1.05C
	Control	1.00±1.82D

*Means followed by same letters within treatments are not significantly different (Tukey test, p=0.05).

 

 

Table II.- Concentration based response (Means± SE*) of biofumigants on rate of adult emergence (number) with comparison to control against R. dominica, T. castaneum and S. oryzae after thirty days.

Test insects	Treatments	F1 progeny (Means± SE*)
T. castaneum	A. indica	35.67±1.37C
	E. camaldulensis	43.11±1.37B
	N. tabacum	18.89±1.37E
	D. stramonium	35.33±1.37C
	C. procera	24.78±1.37D
	Control	55.11±1.37A
R. dominica	A. indica	44.89±2.87AB
	E. camaldulensis	42.56±2.87BC
	N. tabacum	25.11±2.87D
	D. stramonium	35.00±2.87C
	C. procera	44.78±2.87AB
	Control	52.67±2.87A
S. oryzae	A. indica	39.78±2.35C
	E. camaldulensis	50.33±2.35B
	N. tabacum	20.89±2.35E
	D. stramonium	34.00±2.35CD
	C. procera	31.33±2.35D
	Control	63.78±2.35A

*Means followed by same letters within treatments are not significantly different (Tukey test, p=0.05).

 

Biocidal potential in biopesticides

Resistance inheritance in quarantine insect pests populations checked in the previous experiment was also encountered with some bio-pesticides (reported effective in early studies) were used. Significant (P<0.05) effect of bio-pesticides and their concentrations (5, 10 and 15%) was found against all insect pests with exposure time. Plant derived oil of N. tabacum was highly lethal to a lesser grain borer (17.33±1.19SE) and rice weevil (15.33±0.90SE) after 72 h. Even 5% and 10% concentrations produced high toxicity in rice weevil; lessen grain borer and red flour beetle, respectively. Minute lethal effects were observed in oil of E. camaldulensis against all test insects especially red flour beetle proved high resistance at all concentrations and exposure time intervals, respectively. Average survival rate was originated against A. indica and D. stramonium but C. procera was the second most effective bio-pesticides against rice weevil LGB and RFB as shown in Figure 2. Overall results depicted that oils of N. tabacum and C. procera were potential insecticides at 15% rate against phosphine inherited resistant population of quarantine insect pests following by A. indica, D. stramonium and E. camaldulensis, respectively. Tested bio-insecticides have potential and efficient to cope the phosphine resistance inherited strains of these coleopteran beetles.

Progeny production

Although insect respond significantly (P<0.05) against different essential oils but F1 adult emergence rate was little bit high in all insect strains especially in rice weevil in comparison to response observed against phosphine as shown in Table II. Resurgence response in term of offspring development counted in numbers was decline with raise in biofumigants concentrations (15>10>5%) in all insects. However, plant oil concentrations against LGB lessen affected the progeny with respect to red flour beetle. Similarly, among plant oils, N. tabacum and C. procera highly suppressed the progeny development of all insects following by A. indica, D. stramonium and E. camaldulensis, respectively.

 

Discussion

The response to phosphine inherited resistance and important/essential constituents in the (isolated) biopesticides from Nicotiana sp., Azadirachtin sp., Calotropis sp., Eucalyptus sp. and Datura sp. were similar to previous studies performed against red flow beetle, lesser grain borer and rice weevil in different countries. However, results are significantly and/or slightly change with respect to insecticide used, concentrations, and exposure time tested in previous studies parameters. These changes might be due to an environmental variations (geographical, seasonal, climatic, agro ecological position), maturational status of the test animal or phonological state of pesticide source (plant) and soil variations where it was grown, time of year and climatic effects of bio-pesticides or plant part, genetic deference of strains and other chemo types (Nenaah, 2014).

In Australia mild phosphine resistant strains of S. oryzae are common but from some area high resistant weevil population has been documented (Daglish et al., 2014). Weakly phosphine resistant phenotypes of T. castaneum were reported as 62.2% of the collected population of 115 samples. Alarming the resistance development and selection pressure in eastern Australia (Daglish et al., 2015). Stored grains are frequently contaminated in result to exposure stored grain insect pests especially T. castaneum, R. dominica and S. oryzae throughout the storage period. Stored product insect pests are developing behavioral and genetically resistance with progress in the management practices. Two resistant non-sex linked genes (rphl and rph2) were supposed to be responsible for resistance development (Bengston et al., 1999; Price and Mills, 1988). Strange phosphine resistance (431 fold) in quarantine insect pests (T. castaneum) recorded after twenty hours exposure fumigant in sense of insect mortality but was low (12.3 fold) in week resistant strains (Bengston et al., 1999).

Essential oils of Azadirachta sp. and Eucalyptus sp. tested against stored grain insect pests showed potential insecticidal action as well as antimicrobial or anti-oxidant action. United States Food and Drug Administration (FDA) have also categorized these biopesticides as GRAS can be used as food flavors and additives (Prakash et al., 2015). Biological activates of biopesticides with peculiar chemical composition have a range of medical uses (Laribi et al., 2015). Oil of Alpinia purpurata was highly effective against weevil as food deterrent or disrupted the nutritional status but biocidal action was slow when applied on the insect cuticle (Lira et al., 2015). Among previous researches similar response of Azadirachtin (30.68%) and other biopesticides was noted at 20% concentration in beetle mortality while, minimum (20.24%) at 5% neem oil (Hasan et al., 2014). Biopesticides (Spinosad) was lethal to R. dominica resulting 100% mortality at 0.5 mgkg-1exposed 168 hours under controlled conditions (Eissa et al., 2014). Fumigation with oil of Achillea fragrantissima at 60 uL L-1 of air for 12 days exposure caused (91.3%) mortality in S. oryzae and T. castaneum. Lesser grain borer was highly susceptible (100%) at same rate of A. beiber steinii and A. conyzoides. Additionally, powder of mentioned plants when mixed with grains was more effective then oil application against these quarantine insect pests (Nenaah, 2014). Enriched plant extract of A. indica at 1000, 2000 and 4000EC caused successful beetle mortality also made it susceptible to the UV light exposure (Costa et al., 2014). Significant toxicity of level of biopesticides used through topical applicant larval stage and on filter paper for adult red flour beetles recorded after 96 h exposure was 212 uL cm-2 and 796.8 uL cm-2 receptivity at 62.3 mg/mg dose (Nenaah, 2014).

Fumigant toxicity of A. santolina oil was dramatically increased and significant F1 progeny of stored gain beetle was recorded (Nenaah, 2014). Lethal toxicity response of some biopesticides was result in delaying F1 emergence and decrease in growth of the stored product weevil (Jumbo et al., 2014). Percentage emergence of red flour beetle negatively correlated with the concentration of biopesticides. N. tabacum (10%) suppressed the F1 generation (28%) at maximum level as compared to other botanical insecticides. As potential insecticide it affected the F1 by minimizing pupation (40%) (Hasan et al., 2012). Progeny production was censored in term of larvae, pupa and adult emergence of stored product beetle with increase in the concentration of C. procera following by Ammranthus hybridus, Salsola baryosma and Cumminum ciminum (Sagheer et al., 2011). Basil oil of Ocimum basilicum fumigation produced strong lethal toxicity to S. oryzae at 3ml, 10% concentration inside a 1L plastic container. However, biopesticide used in packed rice caused very low mortality. Furthermore, F1 progeny development was unaffected against basil oil fumigation (Follett et al., 2014). Beetle progeny production collected from Faisalabad district of Pakistan was completely failed to develop as compared to Verhari strain against biopesticides extracted from four citrus species. C. aurantium derived insecticide (8%) killed 27.30% adult proved to be highly effective among others (Sagheer et al., 2013).

Similarly, mortality rate was significant maximum (96.67%) when essential oils of two Eugenia sp. were applied on filter paper (Gonzalez et al., 2014). Another example of biopesticides of Myrtales family (Eucalyptus sp.) was found to be litter bit effective against infestation of Sitophilus sp. and Tribolium sp. When tested under laboratory conditions on homogenous population (Tapondjou et al., 2005). Positive and improved synergistic insecticidal action of basil oil with other commercial fumigants, heat and irradiation application was suggested against stored grain insect pests (Follett et al., 2013, 2014). Insecticidal activity of D. stramonium, E. camaldulensis, Moringa deifera and Nigella sativa oils were checked against three coleopteran beetles including T. castaneum and found effective. D. stramonium was highly toxic among all biopesticides treated against beetle and T. castaneum found second most susceptible (17.11%) insect after Cryptolostes furrugines (23.79%), respectively (Saleem et al., 2014). Toxicological effects of N. tabacum at 10% concentration were high (6.69%) against T. castaneum mortality as well as its repellency (93.33%). While, Pegnum hermale and Aussurea costa were least effective so scientists nominated the N. tabacum and Salsola baryosma best for management of stored grain insect pest (Alvi, 2013). Fumigation toxicity of two Eucalyptus sp. against C. maculates was in wide rang (0.9-100%) in progeny production also for morality bioassay (2.58-7.85). Furthermore, female oviposition was highly effected (6.3-100%) (Gusmao et al., 2013). Insect mortality investigated in response to daily concentration variation proved that continuous phosphine treatment with respect to time and dose was less effected as compared diurnal interrupted treatments caused more mortality audit beetles of in the S. oryzae (Beckett, 2011).

 

Acknowledgement

Authors are thankful to the scientists of Pakistan Agriculture Research institute Karachi camps for helping in collection of insect pest strains. Also, thankful to Mr. Raza Ullah from University of Agriculture, Faisalabad for the initial review the research work.

 

Statement of conflict of interest

The authors declare no conflict of interest.

 

References

Alvi, A., 2013. Repellent and toxicological impact of acetone extracts of Nicotiana tabacum, Pegnum hermala, Saussurea costus and Salsola baryosma against red flour beetle, Tribolium castaneum (Herbst). Pakistan J. Zool., 45: 1735-1739.

Ashouri, S. and Shayesteh, N., 2009. Insecticidal activities of Black Pepper and Red Pepper in powder form on adults of Rhyzopertha dominica (F.) and Sitophilus granarius (L.). Pak. Entomol., 31: 122-127.

Beckett, S.J., 2011. Insect and mite control by manipulating temperature and moisture before and during chemical-free storage. J. Stored Prod. Res., 47: 284-292. https://doi.org/10.1016/j.jspr.2011.08.002

Bengston, M., Collins, P.J., Daglish, G.J., Hallman, V.L., Kopittke, R. and Pavic, H., 1999. Inheritance of phosphine resistance in Tribolium castaneum (Coleoptera: Tenebrionidae). J. econ. Ent., 92: 17-20. https://doi.org/10.1093/jee/92.1.17

Benhalima, H., Chaudhry, M., Mills, K. and Price, N., 2004. Phosphine resistance in stored-product insects collected from various grain storage facilities in Morocco. J. Stored Prod. Res., 40: 241-249. https://doi.org/10.1016/S0022-474X(03)00012-2

Betancur, J.G.S., Concepción, A.J., Fischer, S. and Zapta, N., 2010. Insecticidal activity of Peumus boldus molina essential oil against Sitophilus zeamais Motschulsky. J. agric. Res., 70: 399-407.

Brader, B., Lee, R.C., Plarre, R., Burkholder, W., Kitto, G.B., Kao, C., Polston, L., Dorneanu, E., Szabo, I. and Mead, B., 2002. A comparison of screening methods for insect contamination in wheat. J. Stored Prod. Res., 38: 75-86. https://doi.org/10.1016/S0022-474X(01)00006-6

Bughio, F. and Wilkins, R., 2004. Influence of malathion resistance status on survival and growth of Tribolium castaneum (Coleoptera: Tenebrionidae), when fed on flour from insect-resistant and susceptible grain rice cultivars. J. Stored Prod. Res., 40: 65-75. https://doi.org/10.1016/S0022-474X(02)00077-2

Chen, C.Y. and Chen, M.E., 2013. Susceptibility of field populations of the lesser grain borer, Rhyzopertha dominica (F.), to deltamethrin and spinosad on paddy rice in Taiwan. J. Stored Prod. Res., 55: 124-127. https://doi.org/10.1016/j.jspr.2013.10.001

Costa, J.T., Forim, M.R., Costa, E.S., de Souza, J.R., Mondego, J.M. and Junior, A.L.B., 2014. Effects of different formulations of neem oil-based products on control Zabrotes subfasciatus (Coleoptera: Bruchidae) on beans. J. Stored Prod. Res., 56: 49-53. https://doi.org/10.1016/j.jspr.2013.10.004

Daglish, G.J., Nayak, M.K. and Pavic, H., 2014. Phosphine resistance in Sitophilus oryzae (L.) from eastern Australia: Inheritance, fitness and prevalence. J. Stored Prod. Res., 59: 237-244. https://doi.org/10.1016/j.jspr.2014.03.007

Daglish, G.J., Nayak, M.K., Pavic, H. and Smith, L.W., 2015. Prevalence and potential fitness cost of weak phosphine resistance in Tribolium castaneum (Herbst) in eastern Australia. J. Stored Prod. Res., 61: 54-58. https://doi.org/10.1016/j.jspr.2014.11.005

Daglish, G.J. and Pulvirenti, C., 1998. Reduced fecundity of Rhyzopertha dominica. J. Stored Prod. Res., 44: 71-76. https://doi.org/10.1016/j.jspr.2007.06.003

Demissi, G., Tefera, T. and Tadesse, A., 2003. Importance of husk covering on field infestation of maize by Sitophilus zeamais Motsch (Coleoptera: Curculionidae) at Bako Western Ethiopia. Afr. J. Biotechnol., 7: 3777-3779.

Eissa, F.I., Zidan, N.E.H.A., Hashem, M.Y. and Ahmed, S.S., 2014. Insecticidal efficacy of certain bio-insecticides, diatomaceous earth and modified atmospheres against Rhyzopertha dominica (F.)(Coleoptera: Bostrichidae) on stored wheat. J. Stored Prod. Res., 57: 30-35. https://doi.org/10.1016/j.jspr.2014.02.003

Eissa, F.I., Zidan, N.E.H.A., Hashem, M.Y. and Ahmed, S.S., 2014. Insecticidal efficacy of certain bio-insecticides, diatomaceous earth and modified atmospheres against Rhyzopertha dominica (F.) (Coleoptera: Bostrichidae) on stored wheat. J. Stored Prod. Res., 57: 30-35. https://doi.org/10.1016/j.jspr.2014.02.003

Follett, P.A., Rivera-Leong, K. and Myers, R., 2014. Rice weevil response to basil oil fumigation. J. Asia-Pacific Ent., 17: 119-121. https://doi.org/10.1016/j.aspen.2013.11.008

Follett, P.A., Snook, K., Janson, A., Antonio, B., Haruki, A., Okamura, M. and Bisel, J., 2013. Irradiation quarantine treatment for control of Sitophilus oryzae (Coleoptera: Curculionidae) in rice. J. Stored Prod. Res., 52: 63-67. https://doi.org/10.1016/j.jspr.2012.09.004

Fouad, H.A., Faroni, L.R.D., Souza de, T.W., Ribeiro, R.C., Sousa de, F.S. and Zanuncio, J.C., 2014. Botanical extracts of plants from the brazilian cerrado for the integrated management of Sitotroga cerealella (Lepidoptera: Gelechiidae) in stored grain. J. Stored Prod. Res., 57: 6-11. https://doi.org/10.1016/j.jspr.2014.01.001

Gonzalez, M.S., Lima, B.G., Oliveira, A.F., Nunes, D.D., Fernandes, C.P., Santos, M.G., Tietbohl, L.A., Mello, C.B., Rocha, L. and Feder, D., 2014. Effects of essential oil from leaves of Eugenia sulcata on the development of agricultural pest insects. Rev. Brasil. Farmacog., 24: 413-418. https://doi.org/10.1016/j.bjp.2014.05.003

Gusmao, N.M.S., Oliveira de, J.V., Navarro do, A.F., Daniela, M., Dutra, K.A., Silva da, W.A. and Wanderley, M.J.A., 2013. Contact and fumigant toxicity and repellency of Eucalyptus citriodora Hook., Eucalyptus staigeriana F., Cymbopogon winterianus Jowitt and Foeniculum vulgare Mill. essential oils in the management of Callosobruchus maculatus (FABR.) (Coleoptera: Chrysomelidae, Bruchinae). J. Stored Prod. Res., 54: 41-47. https://doi.org/10.1016/j.jspr.2013.02.002

Hanif, C.M.S., Hasan, M.U., Ashfaq, M. and Javed, N., 2013. Combined insecticidal effectiveness of essential oils of two locally grown plant and aluminium phosphide against Tribolium castaneum. Pak. Entomol., 35: 77-81.

Hanif, C.M.S., Mansoor-ul-Hasan, M.S., Saleem, S., Ali, K. and Akhtar, S., 2015. Comparative insecticidal effectiveness of essential oils of three locally grown plants and phosphine gas against Trogoderma granarium. Pak. J. Agric. Sci., 52: 709-715.

Hasan, M., Sagheer, M., Ali, Q., Iqbal, J. and Shahbaz, M., 2012. Growth regulatory effect of extracts of Azadirachta indica, Curcuma longa, Nigella sativa and Piper nigrum on developmental stages of Trogoderma granarium (Everts) (Coleoptera:Dermestidae). Pak. Entomol., 2: 111-115.

Hasan, M., Sagheer, M., Ranjha, M.H., Ali, Q., Hanif, C.M.S. and Anwar, H., 2014. Evaluation of some plant essential oils as repellent and toxicant against Trogoderma granarium (Everts) (Coleoptera: Dermestidae). J. Glob. Innov. Agric. Soc. Sci., 2: 65-69.

Jemâa, J.M.B., Haouel, S. and Khouja, M.L., 2013. Efficacy of Eucalyptus essential oils fumigant control against Ectomyelois ceratoniae (Lepidoptera: Pyralidae) under various space occupation conditions. J. Stored Prod. Res., 53: 67-71. https://doi.org/10.1016/j.jspr.2013.02.007

Jumbo, L.O.V., Faroni, L.R., Oliveira, E.E., Pimentel, M.A. and Silva, G.N., 2014. Potential use of clove and cinnamon essential oils to control the bean weevil, Acanthoscelides obtectus Say, in small storage units. Ind. Crop Prod., 56: 27-34. https://doi.org/10.1016/j.indcrop.2014.02.038

Kwiatkowska, D.P., Nawrot, J., Zielińska-Dawidziak, M., Gawlak, M. and Michalak, M., 2014. Detection of grain infestation caused by the granary weevil (Sitophilus granarius L.) using zymography for α-amylase activity. J. Stored Prod. Res., 56: 43-48. https://doi.org/10.1016/j.jspr.2013.10.005

Laribi, B., Kouki, K., Hamdi, M. and Bettaieb, T., 2015. Coriander (Coriandrum sativum L.) and its bioactive constituents. Fitoterapia, 103: 9-26. https://doi.org/10.1016/j.fitote.2015.03.012

Lira, C.S., Pontual, E.V., de Albuquerque, L.P., Paiva, L.M., Paiva, P.M.G., de Oliveira, J.V., Napoleão, T.H. and Navarro, D.M.A.F., 2015. Evaluation of the toxicity of essential oil from Alpinia purpurata inflorescences to Sitophilus zeamais (maize weevil). Crop Prot., 71: 95-100. https://doi.org/10.1016/j.cropro.2015.02.004

Lira, C.S.D., Pontual, E.V., de Albuquerque, L.P., Paiva, L.M., Paiva, P.M.G., de Oliveira, J.V., Napoleão, T.H. and Navarro, D.M.A.F., 2015. Evaluation of the toxicity of essential oil from Alpinia purpurata inflorescences to Sitophilus zeamais (maize weevil). Crop Prot., 71: 95-100. https://doi.org/10.1016/j.cropro.2015.02.004

Liu, Z.L., Goh, S.H. and Ho, S.H., 2007. Screening of Chinese medicinal herbs for bioactivity against Sitophilus zeamais Motschulsky and Tribolium castaneum (Herbst). J. Stored Prod. Res., 43: 290-296. https://doi.org/10.1016/j.jspr.2006.06.010

Mahmoud, A.K., Satti, A.A., Bedawi, S.M. and Mokhtar, M.M., 2014. Combined insecticidal effects of some botanical extracts against the khapra beetle (Trogoderma granarium Everts). Res. J. Eng. appl. Sci., 3: 388-393.

Nenaah, G.E., 2014. Chemical composition, toxicity and growth inhibitory activities of essential oils of three Achillea species and their nano-emulsions against Tribolium castaneum (Herbst). Ind. Crop. Prod., 53: 252-260. https://doi.org/10.1016/j.indcrop.2013.12.042

Nenaah, G.E., 2014. Bioactivity of powders and essential oils of three Asteraceae plants as post-harvest grain protectants against three major coleopteran pests. J. Asia-Pacific Ent., 17: 701-709. https://doi.org/10.1016/j.aspen.2014.07.003

Ogendo, J., Kostyukovsky, M., Ravid, U., Matasyoh, J., Deng, A., Omolo, E., Kariuki, S. and Shaaya, E., 2008. Bioactivity of Ocimum gratissimum L. oil and two of its constituents against five insect pests attacking stored food products. J. Stored Prod. Res., 44: 328-334. https://doi.org/10.1016/j.jspr.2008.02.009

Pant, M., Dubey, S., Patanjali, P., Naik, S. and Sharma, S., 2014. Insecticidal activity of eucalyptus oil nanoemulsion with karanja and jatropha aqueous filtrates. Int. Biodeterior. Biodegrad., 91: 119-127. https://doi.org/10.1016/j.ibiod.2013.11.019

Pimentel, M., Faroni, L.D.A., Guedes, R., Sousa, A. and Tótola, M., 2009. Phosphine resistance in Brazilian populations of Sitophilus zeamais Motschulsky (Coleoptera: Curculionidae). J. Stored Prod. Res., 45: 71-74. https://doi.org/10.1016/j.jspr.2008.09.001

Prakash, B., Kedia, A., Mishra, P.K. and Dubey, N., 2015. Plant essential oils as food preservatives to control moulds, mycotoxin contamination and oxidative deterioration of agri-food commodities–Potentials and challenges. Fd. Contr., 47: 381-391. https://doi.org/10.1016/j.foodcont.2014.07.023

Price, L. and Mills, K., 1988. The toxicity of phosphine to the immature stages of resistant and susceptible strains of some common stored product beetles, and implications for their control. J. Stored Prod. Res., 24: 51-59. https://doi.org/10.1016/0022-474X(88)90008-2

Rajendran, S. and Sriranjini, V., 2008. Plant products as fumigants for stored-product insect control. J. Stored Prod. Res., 44: 126-135. https://doi.org/10.1016/j.jspr.2007.08.003

Sagheer, M., Hasan, M., Latif, M.A. and Iqbal, J., 2011. Evaluation of some indigenous medicinal plants as a source of toxicant, repellent and growth inhibitors against Tribolium castaneum (Coleoptera: Tenebrionidae). Pak. Entomol., 33: 87-91.

Sagheer, M., Hasan, M., Ali, Z., Yasir, M., Ali, Q., Ali, K., Majid, A. and Khan, F., 2013. Evaluation of essential oils of different citrus species against Trogoderma granarium (Everts) (Coleoptera: Dermestidae) collected from Vehari and Faisalabad districts of Punjab, Pakistan. Pak. Entomol., 35: 37-41.

Sagheer, M., Ali, K., Mansoor-ul-Hasan, Rashid, A., Sagheer, U. and Alvi, A., 2013. Repellent and toxicological impact of acetone extracts of Nicotiana tabacum, Pegnum hermala, Saussurea costus and Salsola baryosma against red flour beetle, Tribolium castaneum (Herbst). Pakistan J. Zool., 45: 1735-1739.

Saleem, S., Hasan, M., Sagheer, M. and Sahi, S.T., 2014. Insecticidal activity of essential oils of four medicinal plants against different stored grain insect pests. Pakistan J. Zool., 46: 1407-1414.

Serin, Z.O., 2016. Interaction of the host’s chemical composition with bark beetles. Transylv. Rev., 24: 12-19.

Sousa, A., Faroni, L.D.A., Pimentel, M. and Guedes, R., 2009. Developmental and population growth rates of phosphine-resistant and susceptible populations of stored product insect pests. J. Stored Prod. Res., 45: 241-246. https://doi.org/10.1016/j.jspr.2009.04.003

Suleiman, R., Williams, D., Nissen, A., Bern, C. and Rosentrater, K., 2015. Is flint corn naturally resistant to Sitophilus zeamais infestation? J. Stored Prod. Res., 60: 19-24. https://doi.org/10.1016/j.jspr.2014.10.007

Tapondjou, A., Adler, C., Fontem, D., Bouda, H. and Reichmuth, C., 2005. Bioactivities of cymol and essential oils of Cupressus sempervirens and Eucalyptus saligna against Sitophilus zeamais Motschulsky and Tribolium confusum. J. Stored Prod. Res., 41: 91-102. https://doi.org/10.1016/j.jspr.2004.01.004

Tapondjou, L., Adler, C., Bouda, H. and Fontem, D., 2002. Efficacy of powder and essential oil from Chenopodium ambrosioides leaves as post-harvest grain protectants against six-stored product beetles. J. Stored Prod. Res., 38: 395-402. https://doi.org/10.1016/S0022-474X(01)00044-3

Zandi-Sohani, N. and Ramezani, L., 2015. Evaluation of five essential oils as botanical acaricides against the strawberry spider mite Tetranychus turkestani Ugarov and Nikolskii. Int. Biodeterior. Biodegrad., 98: 101-106. https://doi.org/10.1016/j.ibiod.2014.12.007

Ziaee, M., Moharramipour, S. and Francikowski, J., 2014. The synergistic effects of Carum copticum essential oil on diatomaceous earth against Sitophilus granarius and Tribolium confusum. J. Asia-Pacific Ent., 17: 817-822. https://doi.org/10.1016/j.aspen.2014.08.001