Detection of Intestinal Fungi in Chickens Naturally Infected with Infectious Bursal Disease
Detection of Intestinal Fungi in Chickens Naturally Infected with Infectious Bursal Disease
Aly M. Ghetas1*, Dalia M. Sedeek1, Hanaa S. Fedawy1, M.A. Bosila1, Hoda M. Mekky1, Kh. M. Elbayoumi1, 3, Mohamed M. Amer2
Intestine tissue sections of naturally IBDV infected chicken stained by H and E. A. Normal intestine tissue sections stained (x 200) B: Chicken intestine showing inflammatory cells infiltration of the mucosa (white arrow) and congestion (C) of the submucosa (x 100). C: Chicken intestine showing inflammatory cells infiltration of the mucosa (white arrow) (x 100).
Amplified ITS gene of isolated fungi. Lane L = 100 bp marker; Lane P = Positive control. Lanes 1 (A. niger), 6 (A. fumigatus), 7 (A. flavus), 9 (A. fumigatus) and 10 (A. flavus); Lane N = Negative control.
Phylogenetic tree 5 Aspergillus strains named MZ052072, MZ052073, MZ048029, MZ048030 and MZ052073 are compared with 21 sequences of ITS gen published in GenBank. Branched distances correspond to sequence divergence.
Percentage of nucleotide identities for the ITS genes of 5 Aspergillus strains named MZ052072, MZ052073, MZ048029, MZ048030 and MZ052073as compared with 21 sequences published in GenBank.
Deduced amino acid sequence of the ITS gene of of 5 Aspergillus strains named MZ052072, MZ052073, MZ048029, MZ048030 and MZ052073 as compared with 21 sequences published in GenBank.
