Asma-ul-Husna1, Muhammad Sajjad Ansari2, Bushra Allah Rakha3, Rabea Ejaz1, Nemat Ullah1 and Shamim Akhter1*
...the role of melatonin in extender for its cryoprotective effect on post-thaw quality of buffalo bull semen. For this purpose, two consecutive ejaculates were collected from three Nili-Ravi buffalo bulls using artificial vagina at weekly intervals for a period of three weeks (three replicates). Qualifying semen ejaculates were diluted (50×106 motile spermatozoa ml-1) in tris citric acid extender with melatonin at 0 (con...
Hamayun Khan1,*, Momen Khan2, Muhammad Subhan Qureshi3, Shakoor Ahmad1, Ali Gohar4, Hameed Ullah5, FarmanUllah6, Arab Hussain7, Pershotam Khatri8, Said Sajjad Ali Shah1, Hamid Rehman1 and Azmatullah Khan1
...was extended in egg yolk extender at various inclusion levels (0.0%, 0.25%, 0.5%, 0.75 and 1.0%) of green tea extract. Testing was consummated for quality characteristics of post-thawed spermatozoa such as motility, viability and plasma membrane integrity. The data was statistically analyzed with one-way analysis of variance using SPSS. The highest significant (P<0.05) response was obtained in extenders containing 0.75% g...
Mushtaq Ahmad1,*, Mehboob Ahmed1,2 and Nasim Ahmad1
...s citrate egg yolk based extender and cryopreserved using standard protocol. The semen straws were thawed at 37oC and stained using different protocols as followed: Protocol 1 (washing effect); washing either with normal saline (NS) or phosphate buffered saline (PBS), Protocol II (heating effect during incubation in tampon solution); smears were immersed in tampon either at room temperature or at 60oC, Protocol III (concentration of dye i...
Asmatullah Kaka2, Wahid Haron1,*, Rosnina Yusoff 1, Nurhusien Yimer1, A.M Khumran1, Akeel Ahmed Memon2, Kazhal Sarsaifi1 andMahdi Ebrahimi3
... were extended into Tris extender consist of equal concentration of each ALA and DHA (0, 3, 5, 10,15ng/ml). Extended semen was cooled at 5oC for 2 hours at 5oC and then packaged in 0.25ml straws and stored into liquid nitrogen for 24 hours. Successively, thawing and evaluation was performed for sperm motility, viability, membrane integrity, morphology, malondialdehyde (MDA), fatty acid composition and super oxide dismutase (SOD). Sperm ch...
Ibrar Muhammad Khan1, Rifat Ullah Khan1,*, Muhammad Subhan Qureshi2, Tahir Usman2, Adnan Khan1, Zakir Ullah1 and Hamid Rehman1
...ly diluted with egg yolk extender and tested for individual motility, progressive motility, plasma membrane integrity, acrosomal and DNA integrity, seminal pH and dead sperm percentage in fresh and thawed semen. Data were subjected to one way analysis of variance. High sperm individual motility (85.12 ± 0.51%), progressive motility (20.25 ± 0.47%), plasma membrane integrity (82.25 ± 0.43%), acrosomal integrity (79.91 ± 0.51%), and D...
Nizam Uddin Jamali1, Asmatullah Kaka1,*, Pershotam Khatri1, Moolchand Malhi2, Muhammad Naeem3, Akeel Ahmed Memon1, Rameez Raja Kaleri4, Habibullah Janyaro5 and Dildar Hussain Kalhoro6
...um addition to the semen extender on chilled and frozen thawed quality of Kundhi buffalo bull semen. After collection ejaculate was brought in Semen Production Unit. Semen was evaluated as fresh for volume, colour, motility, morphology, live dead ratio, membrane integrity and sperm concentration, post chilling for motility, morphology, live dead ratio, membrane integrity and frozen thawed for motility, morphology, live dead ratio and membrane integrity. Semen ...
Zakirullah1, Hamayun Khan1, Ali Gohar2, Haq Amanullah1,*, Muhammad Younas1, Muhammad Tarique Tunio3, Muhammad Saleem Khan4, Ibrar Muhammad Khan1 and Noushad Khan5
... tris-citric acid extender (TCA) was supplemented with various concentrations of L-cysteine 0 mM, 0.5 mM, 1.0 mM and 1.5 mM. Cooling and equilibration of the diluted semen was done at 4°C for 4 h subsequently filled in straws at the same temperature and were cooled in liquid nitrogen vapors for 10 min. followed by storage at -196°C for assessment. Progressive motility and viability of spermatozoa were significantly higher in 1.5 mM and 1.0 mM L-...
Amit Sharma1* and Pankaj Sood2
...ique, collection season, extender composition, type of cryoprotectant used, cooling rate, equilibration time, freezing and thawing rate. All these factors should be given due credit for achieving better post-thaw post thaw quality of semen to be utilized in different advanced reproductive techniques. ...
Bushra Ismail Khan1, Shamim Akhter1, Sanwal Aslam2* and Rabea Ejaz1
... Polyvinylpyrrolidone in extender on cryopreservation of Nili-Ravi buffalo bull semen. The semen samples were collected from Nili-Ravi buffalo (Bubalus bubalis) bull kept at SPU Qadirabad, District Sahiwal, Pakistan. Qualifying semen ejaculates having motility >60%, volume >5-6ml and concentration >0.5 billion/ ml were diluted 50 × 106 motile sperm ml approximately at 37°C in Tris-citric acid extend...
Rabea Ejaz1*, Saima Qadeer2,Muhammad Sajjad Ansari3, Bushra Allah Rakha4, Shazia Shamas5, Iram Maqsood1, Asma Ul Husna6, Asima Azam1 and Shamim Akhter6
... arachidic acid in semen extender. For this purpose, semen was collected from 3 adult buffalo bulls (Bubalus bubalis) of same age by artificial vagina keeping temperature at 42°C for a period of 5 weeks (replicates; n=30). Ejaculates were mixed in tris citric acid extender having 0.0 (control), 20.0, 25.0 and 30.0 ng/mL of arachidic acid at a temperature of 37°C (>1 mL volume, >0.5 billion per mL ...
Haris Setiawan1, Aris Winaya1,*, Herwintono Herwintono1, Mulyoto Pangestu2 and Yayuk Kholifah3
...ntation in tris Egg-yolk extender on mortality and motility of spermatozoa of Limousin bull (Bos primigenius f. taurus [Linnaeus, 1758]) collected from Singosari Artificial Insemination Center, Indonesia and commercial SFE. Semen from the same bull were collected and processed according to the protocol at Singosari AI Centre. Semen extender were supplemented with SFE, G0 = 0 % SFE, G1 = 2 % SFE, G2 = 4 % SFE, G3 = 6 %...
Alamaary Mohaammed Saad1,3, Abd Wahid Haron1*, Mohamed Ali2,
Mark Wen Han Hiew1 and Lawan Adamu1
...semen with the following extenders: HF-20 extender (0) as control, 0.5 cysteine, and 0.5 ascorbic acid. The level of oxidative stress steadily increases with increase concentrations of cysteine and ascorbic acid. Although the samples treated with cysteine and those with low concentrations of ascorbic acid showed lower level of oxidative stress, the control group revealed a much better protection of oxidative stress. The supp...
Noor Bad Shah Khattak1, Hamayun Khan1, Muhammad Tarique Tunio2, Shakoor Ahmad1, Nazir Ahmad3, Syed Murtaza Hassan Andrabi4, Obaidullah5, Ihsan Ali1,5,* and Nan-Zhu Fang5,*
... Egg yolk was used as an extender, in which different concentrations of GT extract (0.0%, 0.50%, 1.0%, 1.5%, 2.0% and 2.5%) were added. Semen quality indicators studied were the percentage of motility, survivability, plasma membrane integrity and acrosome integrity of frozen buffalo bull sperms. The antioxidative effect of GT was observed at the comparatively lower concentration (0.1-1.5%) on the post-thawed quality indicators. On the other hand, the hight GT ...
Muhammad Tariq1, Imtiaz Rabbani2, Muhammad Shahbaz Yousaf2, Imdad Ullah Khan1*, Hafsa Zaneb2, Sajid Iqbal3, Alam Zeb Khan1, Shakirullah1, Muhammad Shuaib Khan1, Atta Ur Rehman1 and Mumtaz Ali Khan2
...of antioxidants in semen extender at the time of cryopreservation. In the current study, semen from four (n=4) healthy Nili Ravi buffalo bulls was collected through artificial vagina and Gallic acid was added to the semen at 1 µM, 15 µM, 30 µM, 45 µM, 60 µM, and 100 µM. A total of six groups were prepared. One group was kept as control where no Gallic acid was added. After the addition of extender
Muhammad Hammad Fayyaz*, S. Murtaza Hassan Andrabi and Muhammad Shafiq Haider
... L-cysteine (CYS) in the extender. In the first experiment, semen samples from each of the five mature bucks were pooled and diluted in Tris-citric acid (TCA) extender containing Glutathione (0.5mM, 1mM), L-cysteine (5mM CYS, 10mM CYS), and control. In experiment 2, semen samples (n=5) were diluted in TCA extender containing differential doses of combined antioxidants (0.5 mM GLU+ 5mM CYS,...
Ezz Ibrahim Khalifa, Ahmed Loly Ibrahim Desoky, Adel AbdElaziz Elbadawy, Gamal Ibrahim El-Emam, Tarek Messlam Mahmoud Mahdy*
... and 2.0 % for ram semen extender during freezing steps. Semen was collected by an artificial vagina from five adult Rahmani rams twice-weekly for five weeks. Semen was pooled, split into five replicates, and prepared to be frozen. In 1st replicate, semen was extended in Tris egg yolk (TEY) is contained synthetic sources of energy (fructose), antibiotics (penicillin and streptomycin), and antioxidants (vitamin E), which served as control ...
Entesar Z. Eliraqy1*, Basant M. Shafik2, Yasser S. Hussein1, Abdelwahab A. Alsenosy3, Emad A. Abd allah1, Mohamed F. Saad1
...rlic extract additive to extender would be useful to improve semen quality and antioxidant enzymes of buffalo-bulls spermatozoa after different processes (cooling and freezing) at variable time.
Keywords | Plant extract, Preservation, Spermatozoa, Garlic Extender, Antioxidant enzymes.
...
Nuzhat Kamal1, Muhammed Sajjad Ansari2*, Bushra Allah Rakha1, Shamim Akhter1, Saima Qadeer1 and Ali Akhter1
...ed with tris-citric acid extender supplemented with different concentration of alanine (5, 10, 15 and 20mM), extender without alanine was kept as control. Extended semen was cooled to 4°C in 2 h and equilibrated for 4 h at 4°C. After equilibration, cooled semen was filled in 0.5 ml French straw at 4°C and kept over liquid nitrogen vapors for 10 min. Then straws were plunged into liquid nitrogen (-196°C) for s...
I Wayan Lanus Sumadiasa*, Enny Yuliani, Lukman Lukman
...n Tris and Citrate-based extenders on spermatozoa quality of Brangus bull after sex-break. Semen samples (twelve ejaculates) were diluted in Tris-egg yolk (TEY) without GF (control group, T1), TEY with 10% GF (T2), Citrate-egg yolk (CEY) with 10% GF (T3), Citrate with 10% egg yolk and 10% GF (T4), then chill-storage. The variables quality evaluated include spermatozoa progressive motility, visibility, and abnormality. Spermatozoa quality was observed every 24 ...
Asmatullah Kaka1*, Wahid Haron2, Nurhusien Yimer3, Abdullah Channo1, Ali Raza Jahejo2, Mahdi Ebrahimi3, Dildar Hussain Kalhoro4
...xtended in Tris-buffered extender (TBE) supplemented with docosahexaenoic acid (DHA). Electro ejaculator was used to collect the semen and ejaculates having ≥ 20 × 109 spermatozoa with an initial motility rate above 70 %, and normal morphology and viability of ≥ 80 % were extended in TBE, to which different concentrations (0, 3, 5, 10 and 15 ng/ml) of DHA were enriched. Semen sample with supplementations were sequentially incubated for 15 minutes ...
Hasanain Jihad Neamah
...am semen (PPE1=5mg +Tris extender, PPE2=10 mg +Tris extender, and PPE3=30 mg +Tris extender), and compared with control group (Tris extender without addition of PPE). Motility of sperm, hypo osmotic swelling test (HOS-Test), and dead sperm percentage were assessed after 2 and 48 hour at preservation. The PPE2 group exhibited the significantly (p≤0.05)...
Sigit Bintara1*, Riyan Nugroho Aji1, Panjono2, Ali Agus3
Chairdin Dwi Nugraha1, Rafika Febriani Putri1, Ari Ardiantoro2, Wike Andre Septian1, Ahmad Furqon3, Kuswati Kuswati1, Nashi Widodo4, Suyadi Suyadi1*
Vui Van Nguyen1, Samorn Ponchunchoovong2, Sajeera Kupittayanant3, Pakanit Kupittayanant2*
...ng antioxidants to semen extender on canine sperm during 12 days of chilling storage. Twenty ejaculates from five American Bully dogs were used. The sperm quality was determined by system automatic sperm analyser for sperm motility parameters, and by confocal laser scanning microscope for sperm membrane integrity parameters. Sperm lipid peroxidation was also analysed through malondialdehyde production. The study found that there was a gradual decrease in all s...
Vui Van Nguyen1, Samorn Ponchunchoovong2, Sajeera Kupittayanant3, Pakanit Kupittayanant2*
...100 µg/ml in semen extenders had a positive impact on sperm quality. Nonetheless, employing essential oils at elevated concentrations of 200 µg/ml exhibited detrimental impacts on the sperm. The optimal dosage for maintaining the integrity of the dog sperm’s membrane and enhancing its property parameters was determined to be a supplementation of 100 µg/ml essential oils, which demonstrated a significant difference from the control treat...
Abdel-Khalek El-Sayed Abdel-Khalek1*, Abdelaziz M. Sakr2, Wael M. Nagy2, Hesham S. Abou-Serie2, Entesar Z. Eliraqy2
...supplementation on semen extender of Egyptian buffalo semen to improve sperm characteristics and antioxidant capacity of sperm medium after cryopreservation. Tris-egg yolk extender with different levels (0, 4, 8, 12, and 14 mg/ml) of propolis ethanolic extract (PEE) were used. Semen was collected once/week for 20 weeks from five buffalo bulls aged 3.5–5 years. The collected semen was pooled and distributed into five eq...
Aeni Nurlatifah1, Anita Hafid1, Pradita Iustia Sitaresmi1, Santoso Santoso1, Rahma Isatirna Anwar1, Mohammad Firdaus Hudaya1, Diana Andrianita Kusumaningrum1, Nurul Azizah1, Dimar Sari Wahyuni1, Herdis Herdis1, Florentina Bety Indah Lupitasari1, Desiana Ade Mahari1, Muhammad Rizal2
...l into the egg yolk tris extender resulted in the greatest preservation of ram sperm quality (P<0.05), such as motility and viability. Hence, the addition of catalase as EZ at a dose of 50 mg/100 ml to the egg yolk tris extender resulted in the highest (P<0.05) percentage of motility, percentage of live spermatozoa, and intact plasma membrane. The NEZ group showed better results (P<0.05) than the NEZ group in mainta...
Rizkie Elvania1, Tintin Rostini1, Sakiman2, Ani Susilawati2, Emilda Adriani2, Abd Malik1*
...e skim milk and egg yolk extender at varying doses on pre-freeze and post-thawed Kalang buffalo semen. Four Kalang buffalo, aged between 6 to 7 years and weighing 500-550 kg, were involved in this investigation. Semen collection was conducted weekly a 12- week period using an artificial vagina. Lycopene was added to the skim milk-egg yolk diluent at concentrations of 1%, 2%, 3%, and 4%, while the control group received no lycopene substitution. The freshly col...
Nawab Ali1, Akeel Ahmed Memon1, Asmatullah kaka1, Amjad Hussain Mirani2, Muhammad Ibrahim Panhwar3*, Nisar Ahmed Solangi1, Kashif Ali Malik1, Fazul U Rahman1, Moin Akhtar Vistro1
...ris-based Egg Yolk (TEY) extender supplemented with various concentrations of selenium, i.e., group A control (0mM), group B (2mM), group C (4mM) and group D (6mM). Results showed significantly (P<0.05) higher motility (78.1±0.22), morphology (86.5±1.08), membrane integrity (85.6±0.30) and live dead ratio (85.3±0.37) for chilled semen in group B than the other groups. Significantly (P<0.05) improved post-thawed motility (68.1&...
Makruf Arif1, Asmarani Kusumawati2*, Kurniawan Dwi Prihantoko3, Agustina Dwi Wijayanti4, Sri Gustari2
...en diluted with the LREY extender using five different treatments: LREY without antioxidant as control (LREY0), LREY supplemented with genistein at 5 µM (LREY5), genistein at 10 µM (LREY10), glutathione at 0.2 mM (LREY2), and glutathione at 0.4 mM (LREY4). The semen was evaluated after dilution, equilibration, and thawing. The evaluation criteria included sperm motility, recovery rate, viability, membrane integrity, and DNA fragmentation. In the po...
Mariana Isti Dwiningsih1, I Ketut Suatha2 and I Ketut Puja3*
... immediately after added extender, experiment 2, 3 and 4 were. The results of the study found that the average motility percentages for semen transported at 0 hours, 1 hour, 2 hours, and 3 hours were 91.00% ± 0.89, 90.83% ± 0.75, 90.17% ± 0.75, and 89.83% ± 0.75, respectively. Similarly, the average viability percentages for the same transport times were 82.00% ± 0.89, 81.66% ± 1.03, 81.66% ± 1.03, and 80.67% &p...
Langgeng Priyanto1,2*, Herdis2, Santoso2, Pradita Iustitia Sitaresmi2,
Tri Puji Priyatno2, Rahma Isartina Anwar2, Desiana Ade Mahari2,
Florentina Bety Indah Lupitasari2, Maman Surachman2,
I Wayan Angga Darmawan2, Arfan Abrar1 and Arum Setiawan3
...ns, and diluted with TEY extender Semen was supplemented with 0 (C0;E0), 5 (C5;E5), 10 (C10;E10), and 15 (C15;E15) mg/mL of each α-tocopherol and ascorbic acid, respectively. Total sperm motility, viability, abnormalities, and semen membrane plasma were analyzed after thawing (%). C5 and E15 extenders resulted in higher total motility (p 0.05) than the other extenders, with the excep...
Abdullah Channo1,2*, Asmatullah Kaka1, Akeel Ahmed Memon1, Mool Chand Malhi3, Muhammad Bakhsh4, Qudratullah Kalwar5, Shakeel Ahmed Tunio6 and Muhammad Ibrahim Panhwar7
... and Tris based egg yolk extender on post-thawed quality of Tharparkar cattle bull semen. The research was carried out at Department of Animal Reproduction, Faculty of Animal Husbandry and Veterinary Sciences, Sindh Agricultural University Tandojam. In this study four fertile Tharparkar bulls (A, B, C and D) having age of 4-5 years were used. A total of 52 (n=13) ejaculates were collected with the help of artificial vagina, after the collection macroscopic (co...
Mohammed Abdulameer Rashid Al-Sarray1, Hasanain Jihad Neamah2*, Sarah Gatea Fayyadh AL-Omairi1
...1 mg O.M. raw powder and extender, P2= 2 mg O.M. raw powder and extender, O1 group= 1 mg O.M. oil and extender, O2 group= 2 mg O.M. oil and extender and the control only extender. The sperm motility, pH level, malonaldehyde level (MDA), and total antioxidant capacity (TAC) were investigated post-preservation (5ᵒ C) a...
Safaa Sabbar Atiyah1*, Ali Abdullah Zuairi Al-Sadoon2, Shifaa Kadhum Jieish2
...a were diluted by a Tris extender and divided into three treatments: T1 with a Tris extender only (control group), T2: 0.02 AEAV, and T3: 0.03 AEAV. Epididymal sperm were cooled at 5 ºC for 0, 24, 48, 72, 96, and 120 hours. The results show a significant effect (P≤0.01) of AEAV on sperm motility, abnormal sperm percentage, dead sperm percentage, and membrane integrity. The overall means were 51.11 %, 65.55 %, and 60....
Sumaya Loay Mohamed Shams Al-Dean1*, Sura Shakir Hammoud2, Mohammedali J. Ghafil3
.... In an egg-yolk citrate extender, the current study evaluates the impact of various vit Cand E concentrations and their fusion on inferior-quality Ram semen. In this investigation, Four Ghezel-Merinose rams, who were roughly two years old, were employed .They were kept in settings of uniform lighting, housing, and food. The farm’s feeding programme was applied to the experimental animals as well. Ejaculate of the rams is divided into seven groups. For t...
Siew Ing Nguang, Nur Syafiqah Binti Zainal, Ahmad Mukhlis Bin Hafas, Hou Chew Ha, Connie Fay Komilus, and Asmad Kari*
...cies requires a specific extender because of diverse sperm characteristics. Tilapia (Oreochromis sp.) popular in Malaysia for its hardiness and rapid growth is an excellent choice for aquaculture. Aloe vera (Aloe barbadensis) (Av) shows promise in spermatogenesis, but its use as a cryoprotectant in semen extenders is still underexplored. This study investigates the effects of antibiotic- and sugar-free Av-based...
Lalu Ahmad Zaenuri*, Rodiah Rodiah
... in a Tris-albumin-based extender on the motility, viability, and morphology of Boer buck spermatozoa. The semen used in this study was collected from a 3-year-old Boer buck weighing 85 kg, which was well-trained, fertile, and free of both internal and external parasites. Ten semen samples were collected using an artificial vagina every four days over a period of ten consecutive days. The collected semen was divided into four tubes based on to the treatment
Sigit Bintara1*, Diah Tri Widayati1 and Pradita Iustitia Sitaresmi2
...olk citrate (2.9% (v/v)) extender containing 1%, 2%, 3%, and 4% (P1; P2; P3; P4), and control/P0 which will then be evaluated for the quality of the semen produced, both in low temperatures (5° C) and frozen in straws that will be thawed later. The data obtained in this study were statistically analyzed using a completely randomized design (CRD). The results showed that the addition of C. xanthorrhiza improved the motility and viability of spermatozoa both...
Lalu Ahmad Zaenuri*, Enny Yuliani
... aged 3 years. The semen extender employed was Tris-egg yolk. The study was conducted using a 3 x 5 Completely Randomized Design (CRD). The first factor examined was the pre-freezing duration, which included 10, 15, and 20 minutes (T1, T2, and T3, respectively). The second factor assessed was the post-thawing storage time of 0, 30, 60, 90, and 120 minutes. After equilibration, Eppendorf tubes containing liquid semen were placed four centimeters above liquid ni...
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