Salma Shaheen, Muhammad Jamil Khan, Saleem Jilani
...ith or without effective microorganism (EM) on the agronomic performance of spinach crop, at Gomal University D.I. Khan during 2009- 11. A commercial product called Bio-Aab was used as a source of EM. Organic manure was first mixed with Bio-Aab, left for 7 to 15 days at room temperature (20oC) and then applied to appropri- ate pots. The treatments of farm yard manure (FYM) @ 10 t ha-1, press mud @ 20 t ha-1, compost @ 0.7 t ha-1 and poultry manure @ 5 t ha-1 w...
Aqeel Ahmad, Zammurad Iqbal Ahmed, Irfan Aziz*
...by phosphorus mobilizing microorganisms 2.5 packets ha-1, in addition to control were evaluated under lentil-sorghum and lentil-mungbean cropping patterns. Fertilizer treatments were only applied to lentil while their residual effect was studied on sorghum and mungbean. The results revealed that net benefits and marginal rate of return from NPK were the highest mainly due to high lentil yield and poultry manure was next to it. The residual analysis ...
Atul Sharma, Aruna Chandra Singh, Gautam Bacher, Sunil Bhand
...or inhibit the growth of microorganisms. Their abuse results in various side effects on the human health, environment and agriculture. The occurrence of bacterial “suprainfection” with tolerance to antibiotics has attracted the significant attention, mainly due to the consequences of multi drug resistance and potential threat to human health and the environment. With the increasing incidences of antimicrobial contamination, especially in food, dair...
Iram Liaqat1*, Najma Arshad2, Muhammad Arshad3, Safdar Ali Mirza4, Nazish Mazhar Ali1 and Ammara Shoukat1
...ivity against all tested microorganisms, while C. sinensis and M. piperita showed weak antimicrobial activity. Methanolic plant extracts exhibited greater antimicrobial activity than aqueous extracts. Maximum zone of inhibition exhibited by methanolic extracts of S. aromaticum was 10 mm and 20 mm in mono and mixed culture bacterial isolates, respectively. The findings from this study warrant further research to help to establish an alternative anti-infective p...
Salma Shaheen, Mumtaz Khan, Muhammad Jamil Khan, Saleem Jilani, Zarina Bibi, Muhammad Munir and Mehwish Kiran
...ditions....
Sanaullah Jalil*, Asim Hayat, Abid Majeed, Syed Haider Abbas, Muhammad Noman, Muhammad Imran Kasana and Muhammad Mazhar Hussain
...rd manure with effective microorganisms would be a promising strategy for enhancing P use efficiency and productivity of wheat crop in eco friendly way....
A. K. Chaubey and Satyandra Kumar
Bio-management of root knot nematode and root rot disease by antagonistic fungi and rhizobacteria
...ontrol (P< 0.05). The microorganisms which caused high nematicidal activity also showed antifungal activity in dual plate culture test in vitro. All the antagonists showed reduction in root knot and root rot disease and promote plants growth as the length and weight of root and shoot in pot trials on tomato plants. Keywords: Antagonistic fungi, root knot nematode, wilt fungus, bio-management
...
Sitansu Pan and Someshwar Bhagat
Biological control of plant diseases
...hat is why consortium of microorganisms is needed for tackling several plant pathogens simultaneously instead of only one biocontrol agent-one pathogen,which is the most common and established system of biocontrol of plant diseases....
Rana Muhammad Aadil1,2*, Xin-An Zeng1, Saqib Jabbar3, Akmal Nazir2, Abid Aslam Mann2, Muhammad Kashif Iqbal Khan2, Abdullah2, Amna Ramzan2
...es, bioactive compounds, microorganisms and enzyme analysis. The grapefruit juice was treated with processing variables of HPP treatment (0, 150, 200 and 250 MPa), temperature (40, 50 and 60 oC), for processing time of 3 minutes and thermal treatment was done in a water bath at 70 oC for 90 seconds. In HPP treatment (250 MPa/60 °C/3 min), significant reduction was observed in microorganisms, PME and...
Sadaf Javaria*, Muhammad Qasim Khan*, Kashif Waseem*, Muhammad Saleem Jilani*, Habib ur Rahman*, Muhammad Sohail Khan* and Muhammad Sohail**
EXPERIMENTAL INVESTIGATION ON IMPACT OF BIO AND ORGANOMINERAL FERTILIZES ON COMPOSITION AND SHELF LIFE OF TOMATO ( ) FRUIT.
...io-fertilizer (effective microorganisms) with or without organic manure (Farmyard Manure) and inorganic fertilizer (NPK) on the quality characteristics of tomato. The pot experiment was conducted by combining 7 treatments in completely randomized design with 3 replications. The results revealed that nutrient sources significantly affected different quality parameters including; physicochemical (vitamin C, lycopene, total soluble solids and pericarp thickness);...
Ewa Czerniawska-Piątkowska1,*, Magdalena Skibicka1, Barbara Cioch-Szklarz1, Jolanta Karakulska2, Karol Fijałkowski2 and Sonia Hiller3
...d absence or presence of microorganisms in the nasal cavity in a small amount (<10 CFU).
...
Syed Aun Muhammad1, Muhammad Soaib Said2, Shams Ur Rehman2, Muhammad Dawood2, Muneer Ahmed Qazi3 and Nighat Fatima2,*
...merging highly resistant microorganisms. The existing beta lactamase inhibitors are considered ineffective against these superbugs and there is an urgent need for discovery of novel drugs with selective potential. In this study, we illuminate the binding mode of pimarane diterpenes (diaporthein A and diaporthein B) to the NMD-1 by molecular docking. A computational ligand-target docking approach was used to analyze structural complexes of enzyme target with di...
Hareem Mohsin, Azka Asif*, Warda Fatima, Sarooj Nadeem, Anjum Nasim Sabri
Assessment of biocalcification by the water absorption test using microorganism from oil contaminated site
...f calcium along with the microorganism under optimum culture conditions. Alkaline conditions produced by the activity of the enzyme urease allowed the reaction between calcium and carbonate ions that leads to the precipitation of the calcium carbonate. The water absorption test was performed to check the presence of a protective layer that provides resistance to water penetration and corrosion to cement. The samples treated with calcium and urea for one hour ...
Dur-E-Shahwar1,2, Riaz Ahmad Sheikh1, Nazia Jamil2*
...ds which are produced by microorganisms that have several applications in petroleum, pharmaceutical and agricultural industries. Produced water is a major waste water stream of petroleum industry which is produced during petroleum extraction from subsurface in which hydrocarbons are found as a main environmental pollutant. Present study is focused on production of biosurfactants from indigenous bacteria, isolated from produced water and produced water contamin...
Zahra Nazir1, Saba Ijaz1, Roquyya Gul2 and Mahjabeen Saleem1,*
...ead in plants, fungi and microorganism, gain commercial importance for improving the yield and nutraceutical properties of juice processing industry, degumming of fibre plants and maximum oil recovery. These enzymes break down complex polysaccharide polymers of plant tissues into simpler monomer like D-galacturonic acids. In this study, polygalacturonase from grape skin was purified by salting out with ammonium sulfate, gel filtration on Sephadex G-75 column a...
Mohammed Al Bratty1,*, Hassan A. Alhazmi1,2, Desam Nagarjuna Reddy3, Abdul Jabbar Al-Rajab3, Sadique A. Javed1 and Zia ur Rehman1,4
...side attackers including microorganisms. In present study, propolis sample was collected from adjacent to Jazan city, Saudi Arabia and the chemical constituents of the ethanolic extract was evaluated by GC-MS. The ethanolic extract was tested against selected strains of microorganisms for its antibacterial and antifungal activities. The major classes of compounds indentified in the extract were fatty acid esters and aliphati...
Shagufta Naz1, Ayesha Javed1, Ayesha Saleem1, Khadija Murtaza1, Rukhama Haq1, Akbar Hayat2 and Neelma Munir1,*
... total demolition of all microorganisms and their spores in foods. In recent study garlic samples were treated with three different doses of gamma radiation such as 0.5, 1 and 2 kGy. Effects of gamma radiation were analyzed for different aspects like microflora, moisture, fat, ash, fiber, phenolic content, sprouting inhibition, physiological weight loss, pyruvate analysis and sugar contents. Gamma radiation affected these parameters at higher doses but at 0.5 ...
Matiullah Khan1*, Motsim Billah2, Shoaib Ahmad1, Raza Ullah Khan1 and Muhammad Sarwar1
...oculating with effective microorganisms (EM) on the wheat during 2010-11. Both experiments were conducted consecutively, in the same lay out of randomized complete block design with three repeats. Various doses of PEC (6, 4, 2 Mg ha-1) were compared with simple poultry-litter compost (PLC) as 8 Mg ha-1, single super phosphate (SSP) fertilizer as 100 kg P2O5 ha-1 and control. The results showed that PEC at 6 Mg ha-1gave 14.7% and 7.7% increased paddy yield and ...
Tanzeela Riaz, Fiza Sana-Ullah Khan, Farah Rauf Shakoori
...kistan. Locally isolated microorganisms were screened for their mannanase producing characteristics on mineral salt agar medium pH 7. Six isolates were found to be mannanase producers by qualitative analysis. Three isolates (MBL-SP02, MBL-CW01 and MBL-GN02) produced maximum zone of hydrolysis with maximum activity ratio. These isolates (MBL-SP02, MBL-CW01 and MBL-GN02) produced maximum units of mannanase activity, i.e., 9.474, 7.364 and 3.568 U/ml in qu...
Barkat Ali1,2, Wasim Sajjad1,2,3, Imran Khan1, Muhammad Rafiq1, Sahib Zada1, Aamer Ali Shah1 and Fariha Hasan1,*
...esistance emerging among microorganisms against antibiotics already in use. Microorganisms from unusual environments, such as reported from the surface and inside of rocks are a repository of certain metabolites that might be able to solve the problem of increasing resistance against antibiotics. The present study was designed to isolate endolithic and epilithic bacterial strains from the rapidly weathering rocks collected f...
Saleha Ashfaq1, Manzoor Hussain1, Nazish Bibi2, Jan Alam1, Muhammad Junaid2 and Sabi-Ur-Rehman3*
...nd n-hexane 26mm against microorganisms studied. Standard antibiotics were used as a positive control for bacteria and fungi respectively. The cytotoxic assay results showed that methanolic extracts of stem and root of H. gilesii had toxic effects on brine shrimp larvae with LD50 values of 116.72 µg/ml and 168.16 µg/ml respectively. The antimicrobial and cytotoxic parameters reported can be considered as quality standards of H. gilesii in herbal in...
Nafeesa Batool1*, Javed Iqbal Qazi1, Nida Aziz1, Ali Hussain2 and Syed Zawwar Hussain Shah3
...settings at substrate to microorganism (S / M) ratio of 1.0 based on volatile solids (VS) for 30 days. Solids [Total solids (TS), volatile solids (VS), fixed solids (FS) and their ratios as (VS / TS)] determinations were also carried out for process monitoring. The highest cumulative biogas yield of 459.4 mL /g VS was observed in the co-digestion of DM + FW with 58.63 and 41.36 % CH4 and CO2 contents. BMP of the microcrystalline cellulose...
Abid Khan*, Mukhtar Alam and Yousaf Jamal
...n sources and beneficial microorganisms on growth and biochemical traits of wheat” for two years at Agricultural Research Station Buner, Khyber Pakhtunkhwa, Pakistan. The experiments were laid out using three factors factorial Randomized Complete Block Design (RCBD) with three replications. Bio-aab was used as a source of (BM) culture. Microbes present in this culture were species of photosynthetic bacteria (Rhodopseudomonas palustris, Rhodobacter spaero...
Abdiaziz Idiris Mohamud1, Yonis Abukar Mohamed2, Osman Sheikh Ali Jama3, Pravin Mishra4* and Mohamed Idiris Mohamed5
Prevalence and Major Pathogens Associated with Clinical and Subclinical Mastitis in Dairy Camel (Camelus dromedarius) in Benadir Region of Somalia
...recognize the associated microorganisms as causal agents of mastitis. The prevalence of this study was measured by using California mastitis test (CMT). Milk samples were collected from the Deyniile District, Benadir Region of Somalia. The overall prevalence of mastitis was 16.66% (7.93% on the quarter basis), the prevalence of clinical and subclinical mastitis was found to be 22.78% and 9.85% on the animal basis and 9.37% and 6.15% on quarter basis, respectiv...
Taqi Raza1*, Sergio de Los Santos Villalobos2, Muhammad Shehzad3, Shakeel Imran4 and Derly José Henriques da Silva5
PGP Characterization of Rhizobacteria Associated with Apple Gourd (Praecitrullus fistulosus L.)
... are beneficial group of microorganisms which improve plant and soil health by enhancing nutrient availability and protecting plant against stresses. The current study was carried out to characterization of rhizobacteria associated with Apple Guard (Praecitrullus fistulous L.). Morphological, qualitative and quantitative tests were performed to characterize the PGPR abilities of isolated rhizobacteria. Lab study indicated that isolated bacterial strains have m...
Ayesha Talib1,2, Khanzadi Nazneen Manzoor1, Wajahat Ali1, Maria Saeed1, Muhammad Asif Gondal1, Malik Badshah3 and Abid Ali Khan1*
...ibiotics in a variety of microorganisms is expanding at an alarming pace. There is a dire need to devise novel tactics to deal with these minute yet evolving ever clever microscopic entities. One of the possible ways to fight them is through nanoparticles. Green synthesis is an easy way to synthesize nanoparticles by using biological resources as it is cost effective, eco-friendly and large-scale production possibilities exist. Copper nanoparticles (CuNPs) hav...
Abdul Nabi Jatt1,2*
...QS) is a system by which microorganisms can monitor their own population density via small signalling molecules to communicate with each other. The sinking of marine snow aggregates is as an essential process for moving organic carbon source from the up-ward to down-ward in the ocean. Marine snow associated bacterial communities perform a crucial role in organic material degradation by producing extracellular hydrolytic enzymes and are considered as key compon...
Rida Haroon Durrani1*, Fareeha Akhtar1, Ali Ahmad Sheikh1, Munazzah Maqbool1, Aleena Kokab1, Kiran Imtiaz2 and Muhammad Ilyas Riaz3
...on. Food spoilage due to microorganisms is a serious issue which causes approximately 25% food loss globally Present study revealed the effects of storage temperature associated with bacterial spoilage of fish meat. A total 24 samples were procured during three different seasons from local retail shops (n=12) and supermarket (n=12) in order to observe the bacteria which are associated with meat spoilage. Total viable count of aerobic psychrophilic, mesophilic ...
Muhamamd Rizwan1*, Muhammad Arshad2, Muhammad Kashif3, Aneela Zameer Durrani4, Asghar Abbas5, Tanveer Ahmad6, Muhammad Nadeem7 , Kinza Khan8
...mechanism of prokaryotic microorganism. It acts as bacterial natural adaptive immune system against phages, plasmids and foreign genomic elements. Mostly prokaryotes uses their CRIPR Cas system to enhance the integrity of their cell membrane that inhibit the permeability of antimicrobials from host body into the bacterial cells. CRISPR Cas system also help bacteria to evade from the host immune system by suppressing the activity of their immune receptors e.g. ...
Shimaa M. Gad1, Ahmed A. Kheder1, Mohamed A. Awad 2
...Phytoplasmas a wall-less microorganism belongs to Class Mollicutes cause diseases in
several commercial ornamental plants, leads to serious economic losses all over the world. During
2017-2018, gazania showing phyllody, yellowing, proliferation, virescence and little leaf symptoms
was observed Giza, Egypt.
Objective: The current work aims the detection and molecular identification of phytoplasma infecting
...
Ahmed A. ElWakil1, Ausama A. Yousif2 , Adel A. Fayed3, Ibrahim M. elsabagh2, Mahmoud
El Gamal2, Omnia H. Refaei3
...commensal and pathogenic microorganisms that
have been reported to replicate in ticks (e.g. Staphylococci, streptococci ) In addition, the analysis
revealed a LSDV sequence output that was nearly equal to sequence output of different bacteria
known to replicate in ticks.
Conclusion: The modified RT-PCR assay used to detect the polyadenylated LSDV DNA polymerase
mRNA in combination with data generated ...
Ljupco Angelovski*, Zagorka Popova, Katerina Blagoevska, Sandra Mojsova, Marija Ratkova Manovska, Mirko Prodanov, Dean Jankuloski, Pavle Sekulovski
...obial resistance of this microorganism towards some critical antimicrobials used in the human and veterinary medicine. In this study samples were taken from three points in the broiler meat production (farm, slaughter line and cold storage of the meat before shipping to the market). A total of 283 samples (cloacal swabs, caeca and carcass swabs) were analyzed for the presence of C. jejuni. The isolates of C. jejuni were confirmed with the con...
Md. Sahidur Rahman1*, Tishita Ape1, Md. Islam1 and Sharmin Chowdhury2
... antimicrobial-resistant microorganisms. We conducted a cross-sectional study to assess the perspective of dairy farmers regarding antibiotic use as well as its residual and resistance effect. We collected data from 101 dairy farmers in Chattogram, Bangladesh. The results revealed that ceftriaxone, gentamicin, streptopenicillin, amoxicillin, and sulfur drugs were the most frequently used antibiotics in the study area. Among the participants, 99% did not follow...
Jun-Dong Tian1,2, Wei-Jie Guo1,2, Han Yan1, Jie Zhang1, Shu-Liao Tian3 and Ji-Qi Lu1,2,*
...ts host rich and diverse microorganisms, and the microbial community could be affected by many factors including activity rhythm which responds to the timing of feces defecation. To test the effect of activity rhythm on fecal microbiota, we used non-invasive sampling method to collect the feces defecated in the morning (AM group) and afternoon (PM group) from a zoo rhesus macaque population. Then 16S rRNA sequencing technology was adopted to assess the microbi...
Yunilas Yunilas1*, Nurzainah Ginting1, Tri Hesti Wahyuni1, Muhammad Zahoor2, Nelzi Fati3 and
Ahmad Wahyudi4
...d technology using local microorganism additives is expected to improve the physical quality of silage feed. This study aimed to determine the effect of adding local microorganisms (MOL) and fermentation time on the physical quality of corn straw silage. This study used a completely randomized design, factorial 3 × 3 with three replications. Factor I was various doses of MOL (1 %, 3 %, 5 %), and Factor II was different...
Nkiru E. Ekechukwu, Felicia N. Ekeh, Chinenye M. Ohanu, Hope C. Ezinwa and Ifeanyi Oscar N. Aguzie*
...s the vector for various microorganisms. They have the potential for spreading diseases in the environment where they are found. Many insects are associated with animal feces and manure, and abattoirs are one of the important sources for such contacts and disease dissemination. We assumed that understanding the diversity of insect fauna might help to shade light on vector insect-borne diseases, prevalence and transmission rates in abattoir communities. Here we...
Mohammad Belal Shaker1, Waleed Rizk El-Ghareeb2,3*, Marwa Magdy Seliem4, Wageh Sobhy Darwish3, Bassam Abdulla Alhawas2, Ahmed E. Tharwat3
...one of the opportunistic microorganisms, that normally inhabits aquatic environments such as fresh, and marine water bodies. Aeromonas spp. might cause food-borne gastroenteritis that might be complicated to cause septicemia, meningitis, endocarditis, and osteomyelitis with high mortalities in immune-compromised persons. This international collaborative research was conducted to investigate the prevalence of Aeromonas spp., particularly A. hydrophila in three ...
Yahia A. Amin1*, Alaa Eldin Z. Mahmoud2, Mohamed Sabry Aref3, Abd El-Latif Shaker Seddek4, Waleed Younis5
...ic to different types of microorganisms responsible for endometritis and its effect on the days open and pregnancy rate. The current study was carried out on 100 Holstein Frisian cows that were not pregnant for 90-120 days after calving. The cows were classified into two groups: the first group is the normal control group (9 cows), and the second group (91 cows) is the treated group and suffered from endometritis. Samples for bacteriological examination were t...
Eman H. Abotalp, Sahar R. Mohamed, Jakeen K. El Jakee
...r owners, and so harmful microorganisms can be easily transmitted from them to human beings. The ongoing review applied to be aware assuming canines and felines in Egypt are colonized with unsafe E. coli serotypes and the antibiotic resistance in these E. coli isolates. A total of 129 rectal swabs were gathered from apparent healthy and diarrheic canines and felines. By using Vitek2 compact system, 42 E. coli isolates (32.6%) were resulted from canines (2...
Husmaini1*, Sabrina1, Firda Arlina1, Linda Suhartati1, Yozella Martilova2
...p>...
Aulia Nurul Saputri, I Komang Gede Wiryawan, Dilla Mareistia Fassah*, Dewi Apri Astuti
...ive for AGP by providing microorganisms that have a health benefit for the host. This study was to identify the molecular characteristics of LAB isolated from the digestive tract of black soldier fly (BSF) larvae on different substrates as probiotic candidates. The LAB from the digestive tract of BSF larvae in chicken manure and palm kernel meal substrates was isolated and taxonomically identified using the 16S rRNA sequence homology and molecular identificati...
Ali Ahmad1*, Zubair Aslam1, Korkmaz Bellitürk2, Ehsan Ullah1, Ali Raza1 and Muhammad Asif3
...ve eliminated beneficial microorganisms, depleted soil fertility, and lowered natural resistance in crops, making them more susceptible to disease and having detrimental consequences for the environment and human health. To address these issues, we must turn to ecologically friendly alternatives like vermicompost tea and vermicompost, which not only increase crop growth and production while lowering diseases and pests over time, but also protect environment an...
Shama Sadaf1*, Komal Hassan1, Ayesha Saeed1 and Zeeshan Ahmad2
... binder. The presence of microorganisms was checked by ASTEM E2149 shake flask method before and after applying antimicrobial finish and after successive 25 washes. The results were analyzed through MANOVA. The fabric properties were checked by using ISO standard test methods. The fabric which was treated with A. indica showed 100% reduction on all samples after 22 hours, but after six days only one colony of microorganism w...
S.A. Khan†, M. Abid and F. Hussain1
...ive sign against harmful microorganisms which are responsible for
considerable losses in agriculture yield.
... 
Nuzhat Munawar1, Abrar Hussain1, Imran Sajid2, Zahra Noreen1, Muhammad Aslam3*, Zeeshan Rehman1 and Aamir Ali3,4
... various disease-causing microorganisms. In the case of well diffusion assay, the plant exhibited a maximum 16mm inhibition zone against B. subtilis a minimum 10 mm inhibition zone against P. aeruginosa, with acetone extract. The same extract seems inactive against K. pneumoniae as compared to control. When tested on S. aureus, chloroform showed a 10 mm inhibition zone while it was inactive against all other experienced bacteria. Ethyl acetate extract gave an ...
Vetlana Aleksandrovna Shemyakova1, Boris Kazievich Laipanov1, Ismail Anatolyevich Bittirov2, Kerim Khasanovich Bolatchiev1, Kamilla Gadzhimuradovna Alieva4, Anatoly Murashevich Bittirov2,3*
Sepri Reski*, Ridho Kurniawan Rusli, Montesqrit, Maria Endo Mahata
SAEED AHMED*1, ZIA-UR-RAHMAN1, SALMA KHALID2, TARIQ KHAN1, ARSHAD IQBAL3
& ZAMARUD SHAH4
...on and identification of microorganisms.Water samples showed the presence of faecal contamination; especially coliform bacteria which is a risk for the health of local community. Salmonella spp. was detected in 7.42 % of water samples having range 1-20 CFU 100ml-1, while E.coli was detected in 91.4% of water samples having colonial population between 1-40 CFU/100ml. According to this study, 98 % of the water sources of study area did not meet the safe limits f...
SADAF SARFRAZ1*, SAFDAR AMEER1, REENA AMBREEN1, SHOMAILA SIKANDAR 2, SHAISTA NAWAZ3 AND YASIR SALEEM3
...bating properties to the microorganisms in various species of cinnamon.
