Asma-ul-Husna1, Muhammad Sajjad Ansari2, Bushra Allah Rakha3, Rabea Ejaz1, Nemat Ullah1 and Shamim Akhter1*
...ted (50×106 motile spermatozoa ml-1) in tris citric acid extender with melatonin at 0 (control), 0.1, 0.5, 1, and 1.5 mM. Diluted semen was cooled to 4°C for 2 h and equilibrated for 4 h at 4°C. Straws were then kept over liquid nitrogen vapours for 10 min and plunged in liquid nitrogen for storage. Thawing was performed after 24 h of storage, at 37°C for 30 seconds and post-thaw sperm quality parameters were assessed. Sperm progressive motil...
Hamayun Khan1,*, Momen Khan2, Muhammad Subhan Qureshi3, Shakoor Ahmad1, Ali Gohar4, Hameed Ullah5, FarmanUllah6, Arab Hussain7, Pershotam Khatri8, Said Sajjad Ali Shah1, Hamid Rehman1 and Azmatullah Khan1
...teristics of post-thawed spermatozoa such as motility, viability and plasma membrane integrity. The data was statistically analyzed with one-way analysis of variance using SPSS. The highest significant (P<0.05) response was obtained in extenders containing 0.75% green tea extract for all studied parameters. Based on the findings of the current study, it can be concluded that supplementation of green tea extract in semen extender showed significant response ...
SHARIF UDDIN, MD. HASIBUL HASAN, MOHAMMED MAHBUB IQBAL, MOHAMMAD AMZAD HOSSAIN
...atocytes, spermatids and spermatozoa stages during the month of June and July.
Irfan1,*, Arshad Javid7, Muhammad Ashraf2, Athar Mahmud3, Muhammad Altaf4, Syed Makhdoom Hussain5, Muhammad Shahbaz4 and Khalid Javed Iqbal6
...ale birds. Mean abnormal spermatozoa rate was 14.61±1.61% with most common acrosome defects 39.67±3.80 % followed by defects in mid-piece 29.61±0.24%, head defects 10.15±1.21% and tail defects 20.57±1.97%. Laid eggs were collected, stored and were incubated for hatching. Hatchability percentage in artificially inseminated hens was greater 88.30% than natural mating 80.51%.
...
Souheila Slimani1,*, Sonia Hamouda1, Chahrazed Souadi1, Sara Silini2, Cherif Abdennour2 and Leila Delimi3
...ed pigeons had more dead spermatozoa compared to the control. Remarkably, abnormal spermatozoa were much higher in the group received 5mg than that of 10mg.The histological profile revealed degenerative changes in testes of treated pigeons with elongated and irregular diameter of seminiferous tubules, degenerative of Sertoli cells, severe atrophy of Leydig cells and pronounced decrease in the interstitial space. However, in ...
Nizam Uddin Jamali1, Asmatullah Kaka1,*, Pershotam Khatri1, Moolchand Malhi2, Muhammad Naeem3, Akeel Ahmed Memon1, Rameez Raja Kaleri4, Habibullah Janyaro5 and Dildar Hussain Kalhoro6
Zakirullah1, Hamayun Khan1, Ali Gohar2, Haq Amanullah1,*, Muhammad Younas1, Muhammad Tarique Tunio3, Muhammad Saleem Khan4, Ibrar Muhammad Khan1 and Noushad Khan5
...otility and viability of spermatozoa were significantly higher in 1.5 mM and 1.0 mM L-cysteine supplemented groups in comparison with 0.5 mM or 0 mM. The number of supra-vital hypo-osmotic swelling test positive spermatozoa at 1.5 mM and 1.0 mM was greater in comparison with lower concentration of 0.5 or 0 mM L-cysteine. Furthermore, significant improvement for acrosomal integrity was recorded in semen samples treated with 1...
Riaz Hussain Pasha1*, Muhammad Zubair Anjum2, Imran Ullah2, Muhammad Akram Khan3, Adnan Ali4, Saif-Ur-Rehman5, Sana Batool2 and Arslan Emmanuel2
...g summer. Free oozing of spermatozoa in testes and ovaries packed with fully grown eggs are the distinct features of this phase. The highest values of Gonadosomatic Index (GSI) for male and female in summer coincided with the histological structure of the gonads where they are in their spawning phase. Present study revealed that C. garua breed once in a year during summer season and this information will be helpful in culturing of this economically important c...
Amit Sharma1* and Pankaj Sood2
...functional damage to the spermatozoa leading to reduced motility, viability, impaired transport and fertility. Caprine sperms are extremely sensitive to cryopreservation compared to other species which is mainly attributed to the compositional variation of the sperm plasma membrane. Different factors affect affects the semen cryopreservation viz. species, breed, semen collection technique, collection season, extender composition, type of cryoprotectant used, ...
Alicja Kowalczyk1, Marian Kuczaj2, Anna Szul3 and Ewa Czerniawska-Piątkowska4*
... of frozen-thawed bovine spermatozoa. In this study, ten Polish Holstein-Friesian bulls with an average age of 3.5 ± 0.5 years were housed individually in pens. From each bull, two ejaculates were collected using an artificial vagina. Semen samples that showed more than 60% motility and 60% viability were selected for this experiment. To the four diluted samples were added 0µg/mL successively; 2µg/mL; 4µg/mL and 8µg/mL buserelin ...
Rabea Ejaz1*, Saima Qadeer2,Muhammad Sajjad Ansari3, Bushra Allah Rakha4, Shazia Shamas5, Iram Maqsood1, Asma Ul Husna6, Asima Azam1 and Shamim Akhter6
...of cryopreserved buffalo spermatozoa by addition of arachidic acid in semen extender. For this purpose, semen was collected from 3 adult buffalo bulls (Bubalus bubalis) of same age by artificial vagina keeping temperature at 42°C for a period of 5 weeks (replicates; n=30). Ejaculates were mixed in tris citric acid extender having 0.0 (control), 20.0, 25.0 and 30.0 ng/mL of arachidic acid at a temperature of 37°C (>1 mL volume, >0.5 ...
Qianbao Wang, Zhengyang Huang, Shoufeng Li, Huayun Huang, Chunmiao Li and Zhenhua Zhao*
...erm motility and useable spermatozoa/ejaculate before 32 wk of age (p>0.05). Feed quantity was correlated positively with sperm concentration and sperm motility. At 50 wk of age, higher sperm motility was observed in males fed with 135.5and 125.5 g/d diet than males fed with 115.5 g/d diet (p<0.05). While feed quantity was correlated positively with semen volume, sperm concentration, useable spermatozoa/ejaculate, nega...
Ibrar Muhammad Khan1, Dezhi Xu1, Zubing Cao1, Hongyu Liu1, Adnan Khan2, Sajid Ur Rahman3, Jam Zaheer Ahmed4, Muhammad Akmal Raheem5 and Yunhai Zhang1*
...s) to semen diluent. The spermatozoa freeze-thawed quality were assessed and found that total motility, progressive motility, membrane functionality, acrosomal integrity, and viability percentage were significantly higher (P < 0.05) in the diluent groups treated with 7.5 mmol of L-cysteine or 4.8 mmol of vitamin E. Correspondingly the DNA integrity and mitochondrial membrane potential percentage were found significantly higher (P < 0.05) in...
Haris Setiawan1, Aris Winaya1,*, Herwintono Herwintono1, Mulyoto Pangestu2 and Yayuk Kholifah3
...protects the membrane of spermatozoa. This study aimed to observe the effects of Snakehead Fish (Channa striata [Bloch 1793]) Extract (SFE) supplementation in tris Egg-yolk extender on mortality and motility of spermatozoa of Limousin bull (Bos primigenius f. taurus [Linnaeus, 1758]) collected from Singosari Artificial Insemination Center, Indonesia and commercial SFE. Semen from the same bull were collected an...
Alamaary Mohaammed Saad1,3, Abd Wahid Haron1*, Mohamed Ali2,
Mark Wen Han Hiew1 and Lawan Adamu1
Noor Bad Shah Khattak1, Hamayun Khan1, Muhammad Tarique Tunio2, Shakoor Ahmad1, Nazir Ahmad3, Syed Murtaza Hassan Andrabi4, Obaidullah5, Ihsan Ali1,5,* and Nan-Zhu Fang5,*
...zen buffalo bull’s spermatozoa. Semen samples were obtained from the bulls for three weeks through artificial vagina. Egg yolk was used as an extender, in which different concentrations of GT extract (0.0%, 0.50%, 1.0%, 1.5%, 2.0% and 2.5%) were added. Semen quality indicators studied were the percentage of motility, survivability, plasma membrane integrity and acrosome integrity of frozen buffalo bull sperms. The antioxidative effect of GT was observed ...
Nour El-Hoda Khayrat Hammad1*, Yousef Y. El-Seady3, Azza E. Hassan1, Sara T. Elazab2, Magdy S. Amer2
...rone hormonal levels and spermatozoa (viability and motility) which accompanied by some histopathological damage in testes and epididymis. On a molecular basis, mRNA expression of CYP17A1 and LHR genes were significantly reduced in testicular tissues of FCZ group while aromatase gene was elevated. The administration of LIN oil after FCZ treatment markedly improved the aforementioned alterations caused by FCZ. So linseed oil is capable to ameliorate the flucona...
Muhammad Tariq1, Imtiaz Rabbani2, Muhammad Shahbaz Yousaf2, Imdad Ullah Khan1*, Hafsa Zaneb2, Sajid Iqbal3, Alam Zeb Khan1, Shakirullah1, Muhammad Shuaib Khan1, Atta Ur Rehman1 and Mumtaz Ali Khan2
...ng cryopreservation, the spermatozoa faces osmo-chemical, mechanical and thermal stresses, which are predominant at dilution, cooling, equilibration, freezing and thawing stages. They damage functional and morphological characteristics of sperms. Beside these exogenous stresses there is an oxidative stress which damages the spermatozoa endogenously. These stresses can be controlled with the inclusion of antioxidants in semen...
Emtenan M. Hanafi1*, Fouad M. Tawfeek2, Walid S. El Nattat1, Moetazza M. Alshafei3, Seham S. Kassem3, Kawkab A. Ahmed4, Enas N. Danial5, Hagar Elbakry3, Hoda S. El-Sayed2
...nimals in G2 showed good spermatozoa profile while, those fed SP (G3) showed low sperm count and motility. Histopathological examination declared testicular degeneration in G1 and G3 and a high spermatogenesis in G2 compared to control group. It was concluded that spirulina may act as health potentiator but also, may negatively affect the male reproductive performance. Special care should be paid for the dose and frequency of administration of any polyphenol o...
Muhammad Hammad Fayyaz*, S. Murtaza Hassan Andrabi and Muhammad Shafiq Haider
...osurvival of beetal buck spermatozoa with the supplementation of Glutathione (GLU) and L-cysteine (CYS) in the extender. In the first experiment, semen samples from each of the five mature bucks were pooled and diluted in Tris-citric acid (TCA) extender containing Glutathione (0.5mM, 1mM), L-cysteine (5mM CYS, 10mM CYS), and control. In experiment 2, semen samples (n=5) were diluted in TCA extender containing differential doses of combined antioxidants (0.5 mM...
Tinda Afriani*, Zaituni Udin, James Hellyward, Endang Purwati, Adisti Rastosari, Dwiki Wahyudi
Lukman Affandhy*, Muchamad Luthfi*, Yeni Widyaningrum, Dian Ratnawati, Mutia Primananda
...ty, individual motility, spermatozoa concentration, viability, and libido. Data were analyzed using the ANOVA test. The results showed that the volume and pH of semen did not differ in all treatments, but treatment A showed cream color and thick consistency over treatments B and C. The mass motility and sperm concentration of treatment C with the dose and period of GnRH administration resulted in the lowest values significantly different (P<0.05) than treat...
Entesar Z. Eliraqy1*, Basant M. Shafik2, Yasser S. Hussein1, Abdelwahab A. Alsenosy3, Emad A. Abd allah1, Mohamed F. Saad1
...ogressive motility, live spermatozoa and acrosome integrity % were higher (P < 0.05) in G1, G2 and G3 with reduced mortality percentage than control group after cooling process. In addition, a significantly increased (P < 0.05) of progressive motility, acrosome integrity and alive spermatozoa percentage in groups treated with different concentrations of garlic extract than control group. While both the abnormality and ...
Nuzhat Kamal1, Muhammed Sajjad Ansari2*, Bushra Allah Rakha1, Shamim Akhter1, Saima Qadeer1 and Ali Akhter1
... quality of Sahiwal bull spermatozoa. Qualifying semen ejaculates [motility (>60%) volume (>4ml) and concentration (>0.5 billion/ml)] collected from three Sahiwal bulls were diluted with tris-citric acid extender supplemented with different concentration of alanine (5, 10, 15 and 20mM), extender without alanine was kept as control. Extended semen was cooled to 4°C in 2 h and equilibrated for 4 h at 4°C. After equilibration, cooled semen was fi...
Ibrahim El-Ratel1*, Mostafa El-Moghazy2, Amira El-Gaml2, Ibrahim Abu El-Naser2
...ibido and abnormality of spermatozoa, while increased semen size and percentages of motility, acrosome status, integrity of membrane, total sperm output, sperm concentration, sperm mitochondrial potential, and initial semen fructose. All PEE levels increased serum follicle-stimulating hormone, luteinizing hormone, testosterone, immunoglobulin, and antibody titer, while reduced lysozyme. All PEE levels improved antioxidants capacity, while reduced malondialdehy...
I Wayan Lanus Sumadiasa*, Enny Yuliani, Lukman Lukman
...trate-based extenders on spermatozoa quality of Brangus bull after sex-break. Semen samples (twelve ejaculates) were diluted in Tris-egg yolk (TEY) without GF (control group, T1), TEY with 10% GF (T2), Citrate-egg yolk (CEY) with 10% GF (T3), Citrate with 10% egg yolk and 10% GF (T4), then chill-storage. The variables quality evaluated include spermatozoa progressive motility, visibility, and abnormality.
Asmatullah Kaka1*, Wahid Haron2, Nurhusien Yimer3, Abdullah Channo1, Ali Raza Jahejo2, Mahdi Ebrahimi3, Dildar Hussain Kalhoro4
...ing ≥ 20 × 109 spermatozoa with an initial motility rate above 70 %, and normal morphology and viability of ≥ 80 % were extended in TBE, to which different concentrations (0, 3, 5, 10 and 15 ng/ml) of DHA were enriched. Semen sample with supplementations were sequentially incubated for 15 minutes at 37 ºC and cooled at 5 ºC for 2 hour than packed in 0.25 ml straws, and cryopreserved in liquid nitrogen for 24 hours. Consequently, straws w...
Makruf Arif1*, Claude Mona Airin1, Dwi Sunu Datrianto2, Dinda Anggun Roro Sejati3
...cludes an examination of spermatozoa motility using a microscope, the viability with the eosin-negrosin staining method, and membrane integrity using the hypoosmotic swelling test (HOST) at the fresh semen, 5th, 24th, and 48th hours post-cold storage. The results revealed that in the 5th hour, percentage of motility, viability, and membrane integrity were not significantly different. However, at the 24th and 48th-hour results revealed significant differences b...
Aulia Puspita Anugra Yekti1, Sri Rahayu3, Gatot Ciptadi2, Trinil Susilawati2*
...ty and the proportion of spermatozoa X and Y from sexed semen using the Percoll Density Gradient Centrifugation method. The quality observed included individual motility, viability, abnormalities, concentration, total motile spermatozoa, chromatin damage, intact acrosome hood of spermatozoa, and sperm capacitation. The treatments consisted of T1 = unsexed semen, T2 = sexed semen X-bearing ...
Tinda Afriani*, Jaswandi, Adisti Rastosari, Muhammad Cadilac Al Razzak, Dwiki Wahyudi
...erved variables included spermatozoa motility, spermatozoa survival, spermatozoa abnormalities and intact plasma membranes of spermatozoa. The obtained data was analyzed using the Analysis of Variance (ANOVA). The results showed that the addition of tomato juice extract into egg yolk citrate diluent exhibited a 20% increase in sperm motility percentage, ...
Jaswandi, Ananda*, Rusfidra, Kusnadidi Subekti, Harif Gusdinal, Raziah Sri Wahyuni, Fahmi Ariansyah Caniago
...ation with variations in spermatozoa concentrations. Semen from 12 KBC was collected and evaluated to determine their characteristics (motility, viability, concentration, and abnormalities). Semen showing motility >70% was diluted into 500µl of Ringer’s lactate solution with various concentrations of spermatozoa, namely 50×106, 100×106, and 150×106. Fertile period and fertility were evaluated...
Lalu Ahmad Zaenuri*, Rodiah Rodiah, Adji Santoso Dradjat, Oscar Yanuarianto, I Wayan Lanus Sumadiasa , Lukman Hy
...valuated for volume, pH, spermatozoa concentration/ml and concentration/ejaculates, plasma membrane integrity, viability, progressive motility, and morphology, including normal and abnormal spermatozoa. The statistical significance of the results was evaluated by pre- and post-test analyses using T-test with repeated measuring. Data were expressed as Means ± SD and SEM. Results revealed that semen volume,
Sigit Bintara1*, Riyan Nugroho Aji1, Panjono2, Ali Agus3
...ion. Results showed that spermatozoa diluted with egg yolk citrate supplemented with 3% lycopene (P3) had better quality than that found in the other four treatments, namely motility before freezing (70.86%) and post thawing (53.00%); viability before freezing (76.00%) and post thawing (60. 14%); plasma membrane integrity before freezing (71.29%) and post thawing (59.86%); and also showed the lowest abnormality level before freezing and post-thawing, (17.29%) ...
Chairdin Dwi Nugraha1, Rafika Febriani Putri1, Ari Ardiantoro2, Wike Andre Septian1, Ahmad Furqon3, Kuswati Kuswati1, Nashi Widodo4, Suyadi Suyadi1*
...ethane on the quality of spermatozoa. Bulls aged 3–4 years used in this research. This research used an experimental method to examine the effect of genistein levels in the diluent on the characteristics of spermatozoa during pre-freezing at 4–5 0C and post-thawing (PT). The data obtained in the form of quantitative data were analyzed descriptively and analysis of variance patterns in One-Way ANOVA. 0 µM, 1...
Khalid M. Al-Syaad1,2
...city (TAC) and number of spermatozoa compared with control group. The histological examination of the kidney revealed alterations induced by CC, including atrophy, tubular necrosis, enlargement of the glomeruli, dilatation and congestion of the glomerular capillaries (G) and the blood vessels around the renal tubules, atrophy in some renal glomeruli, hemorrhage, and around edema interstitial the blood vessels. It is concluded that utilizing propolis with CC co...
Abdel-Khalek El-Sayed Abdel-Khalek1*, Abdelaziz M. Sakr2, Wael M. Nagy2, Hesham S. Abou-Serie2, Entesar Z. Eliraqy2
...nd acrosome integrity of spermatozoa in pre-freezing and post-thaw semen. All PEE-extenders increased (P < 0.05) viable sperm percentage, and level of total antioxidant capacity and superoxide dismutase activity, and decreased percentage of apoptosis, early apoptosis, necrosis, and malondialdehyde level; most favorable results were recorded with PEE2. The foregoing results indicated that supplementation of Tris-semen extender with propolis ethanolic extende...
Omar Berkani1*, Souheila Slimani2, Nora Sakhraoui3 and Cherif Abdennour1
...al period, while most of spermatozoa were malformed or dead. Various histopathological alterations including a decrease in interstitial space, an enlarged lumen, a remarkable atrophy of Leydig cells and malformed spermatozoa were observed in the gonads of birds exposed to CYP. The FT4 concentration decreased significantly and none of the CYP groups was molting. Treatment with PO aqueous extract seems counteracted the toxic e...
Aeni Nurlatifah1, Anita Hafid1, Pradita Iustia Sitaresmi1, Santoso Santoso1, Rahma Isatirna Anwar1, Mohammad Firdaus Hudaya1, Diana Andrianita Kusumaningrum1, Nurul Azizah1, Dimar Sari Wahyuni1, Herdis Herdis1, Florentina Bety Indah Lupitasari1, Desiana Ade Mahari1, Muhammad Rizal2
...idants on the quality of spermatozoa in chilled, stored liquid semen from Garut rams. The rams were randomly divided into seven treatments in a 2 × 3 factorial design. The two factors were the antioxidant levels of EZ (25, 50, and 75 mg) and NEZ (5, 10, and 20 mg). Motility, viability, and plasma membrane integrity were the metrics used in this analysis. The experiment conducted with the additional NEZ on the 4th day of observation showed that the additi...
Lukmanhy1*, Enny Yuliani1, I Wayan Lanus Sumadiasa1, Lalu Ahmad Zaenuri1, Mardiansyah2
...ty, and abnormalities of spermatozoa. The data obtained were analyzed using the analysis of variance (ANOVA) followed by Duncan’s multiple range test to determine the difference among the treatments. The current study showed that adding 15% sugarcane (T2) improved the semen parameters compared to the other treatments with 54% progressive motility, 74.8% viability, and 8.8% total abnormalities at 5°C for 4 days of storage. 15% sugarcane juice in egg y...
Rizkie Elvania1, Tintin Rostini1, Sakiman2, Ani Susilawati2, Emilda Adriani2, Abd Malik1*
...ffalo was the quality of spermatozoa post-thawed. Therefore, research was conducted on the use of lycopene as an effort to improve the quality of spermatozoa post-thawing. This study aimed to assess the impact of incorporating lycopene into both the skim milk and egg yolk extender at varying doses on pre-freeze and post-thawed Kalang buffalo semen. Four Kalang buffalo, aged between 6 to 7 years and weighing 500-550 kg, were ...
Rahmat Rahmat1, Muhammad Yusuf2*, Abdul Latief Toleng2, Herdis Herdis3, Athhar Manabi Diansyah2, Hasrin Hasrin4
.../b>. ...
Budianto Panjaitan1, Husnurrizal Husnurrizal2, V.Y. Edward3, D.A. Nishcaya3, Teuku Armansyah4, Tongku Nizwan Siregar2, Mulyadi Adam5, Arman Sayuti1, Amalia Sutriana4, Hafizuddin Hafizuddin2*
...ISA) method. The average spermatozoa volume (mL); concentration (106 cells/mL); and testosterone concentration (ng/mL) in P1 vs. P2, were 4.82±1.93 vs. 4.92±2.45; 889.0±250.50 vs. 721.0±246.25 (P<0.05); and 8.352.34 vs. 9.60±2.24 (P>0.05), respectively. It is concluded that the PGF2α injection 1 hour prior to the collection was able to increase the spermatozoa concentration comp...
Khaeruddin1, Gatot Ciptadi2, Muhammad Yusuf3, Iswati4, Setya Budhi Udrayana4, Sri Wahjuningsih2*
... quality of Gaga chicken spermatozoa during liquid storage. The semen was collected using abdominal massage methods and divided into 4 tubes filled with diluent. For the analysis, the diluent selected was egg yolk Ringer’s lactate added with BHT at concentrations of 1, 2, and 3 mM, respectively as well as a control treatment without BHT. Liquid semen was stored for 72 hours at 5°C, with spermatozoa quality monitore...
Hilwah Nora1,2, Rajuddin2*, Hafizuddin3, Rachmad Suhanda4, M. Fathurrahman5
...iminary studies based on spermatozoa quality parameters, morphology, and morphometry of testes and seminal vesicles that have never been reported. The main objective of this research is to examine the effects of curcumin on several aspects of reproduction and determine the optimal dose for using curcumin as an antifertility agent. The test animals in this study were Wistar rats (Rattus norvegicus) three-month-old males weighing 300 g. Rats were acclimatized fo...
Kenyum Lollen1, Judith Betsy C1*, Cheryl Antony2 and Stephen Sampath Kumar J3
Aziz Ahmed1*, Anila Naz Soomro2, Muhammad Ramzan Ali1, Muhammad Aleem Khan1 and Hasina Basharat1
...r for the peculiarity of spermatozoa. Present research evaluated the effect of two laboratory-prepared media and one commercially available media i.e., ACTi fish while control is freshwater on sperm quality parameters i.e., percent motility, motility interval, sustainability, and fertilization rates in Nile tilapia (Oreochromis niloticus). The experimental design was CRD with four treatments and three replicates. Lab prepared (Activation media A and B), one co...
Atikah Nur Baity1, Noni Ashri Maghfiroh1, Syalsa Bella Fitriana1, Kurniawan Dwi Prihantoko2, Dyah Maharani2, Diah Tri Widayati2*
...ng. The integrity of the spermatozoa cell DNA affects the success of embryo development. DNA damage carried by the paternal line has an impact on the syngamy process, in which a certain amount of male and female gamete chromosomes unite to continue the division process. This study aimed to determine the effect of storage periods in liquid nitrogen on DNA fragmentation of post-thawed semen of Bali bull. This research was conducted at the Laboratory of Animal Ph...
Husnurrizal Husnurrizal1,2, Hafizuddin Hafizuddin2*, Sri Wahyuni3, Cut Nila Thasmi2, Tongku Nizwan Siregar2,4
...α (PGF2α) on spermatozoa quality improvement still shows inconsistent results. In addition, the mechanism leading to increased spermatozoa concentration after administration of PGF2 has not been clearly explained. Therefore, this research aimed to assess the impact of the time interval between PGF2α administration and semen collection on the enhancement of spermatozoa qua...
Langgeng Priyanto1,2*, Herdis2, Santoso2, Pradita Iustitia Sitaresmi2,
Tri Puji Priyatno2, Rahma Isartina Anwar2, Desiana Ade Mahari2,
Florentina Bety Indah Lupitasari2, Maman Surachman2,
I Wayan Angga Darmawan2, Arfan Abrar1 and Arum Setiawan3
...ne plasma in post-thawed spermatozoa (p 0.05). Finally, compared with the other extenders, abnormalities were lower in the C5-and E10 extenders (p<0.05). Finally, the current findings show that C5 and E15 can increase the quality of post-thawed Brahman bull spermatozoa....
Kusuma Adhianto*, Tina Rahmawati, Rio Ramanda, Muhtarudin Muhtarudin, Sri Suharyati
...roscopic (motility, live spermatozoa, abnormalities, and concentration) quality of sheep semen. The research involved an experimental study using a Randomized Complete Block Design (RCBD) consisting of 3 treatments, with each treatment being replicated 5 times. The treatments applied were as follows: P0; Complete Feed 60% + silage 40% (without the addition of natural and synthetic vitamin E), P1; Complete Feed 60% + Silage 40% + Natural Vitamin E 50 IU (equiva...
Safaa Sabbar Atiyah1*, Ali Abdullah Zuairi Al-Sadoon2, Shifaa Kadhum Jieish2
...(AEAV) to the epididymal spermatozoa of local goats during the cooling process for different periods. The AEAV was prepared and stored in the refrigerator until the time of use. Epididymal spermatozoa were diluted by a Tris extender and divided into three treatments: T1 with a Tris extender only (control group), T2: 0.02 AEAV, and T3: 0.03 AEAV. Epididymal sperm were cooled at 5 ºC for 0, 24, 48, 72, 96, and 120 hours. ...
Khaeruddin1,2, Gatot Ciptadi1, Muhammad Yusuf3, Hermawansyah2, Sahiruddin3 and Sri Wahjuningsih1*
...g the quality of chicken spermatozoa. The aim of this study was to determine the effect of the use of three types of crystallized liquids and two types of monosaccharides on the quality of Gaga chicken spermatozoa. This study used a completely randomized design with a factorial pattern with the first factor being the type of crystalloid liquid (Ringer’s lactate, Ringer’s acetate, and KA-EN 3B) supplemented with e...
Siew Ing Nguang, Nur Syafiqah Binti Zainal, Ahmad Mukhlis Bin Hafas, Hou Chew Ha, Connie Fay Komilus, and Asmad Kari*
...eased motility with most spermatozoa displaying strong vibration. In contrast, T2 and T3 experienced a greater decline in motility with a weaker vibration. Antibiotics in T2 likely contributed to the higher motility observed in this treatment. In summary, the absence of antibiotics and sugar negatively affected sperm viability and motility. The addition of antibiotic, sugar, and Av in the extender proved crucial for maintaining the viability and motility of cr...
Lalu Ahmad Zaenuri*, Rodiah Rodiah
... morphology of Boer buck spermatozoa. The semen used in this study was collected from a 3-year-old Boer buck weighing 85 kg, which was well-trained, fertile, and free of both internal and external parasites. Ten semen samples were collected using an artificial vagina every four days over a period of ten consecutive days. The collected semen was divided into four tubes based on to the treatment extenders: FF0 (0 ml), FF1 (4 ml), FF2 (6 ml), and FF3 (8 ml) of fi...
Sigit Bintara1*, Diah Tri Widayati1 and Pradita Iustitia Sitaresmi2
...otility and viability of spermatozoa both in freeze-thawed semen (p < 0.05). P3 (3% supplementation) resulted in the highest motility (76.14±1.35% pre-freezing; 60.00±1.63% after thawing) and viability (85.00±2.00% pre-freezing and 70.14±1.57% after thawing) p<0.05). Regression tests using cubic curves showed that the R square2 values on motilities and viabilities were higher than 0.85, and could be used as a predictor of the ...
Muhammad Tayyab Khan1*, Muhammad Irfan-ur-Rehman Khan1, Tehmina Yasmin2, Ghulam Shabbir Khan1 and Amjad Riaz1*
...ity and viability (%) of spermatozoa increased (P< 0.05) in HDG and LDG treated mice than those of CON mice whereas sperm concentration was more (P< 0.05) in LDG than HDG and CON treated mice during the treatment. Five weeks after the treatment, motility and viability of spermatozoa were greater (P< 0.05) in HDG and LDG than CON treated mice. In conclusion, Ginseng treatment in aged BALB/c mice enhances the epididym...
Ibrahim Elmaghraby1, Zeinab Said2, Marwa Darweish1, Doaa Galal El-Sahra3 and Ahmed Ibrahim El-Nemr4*
...ion, and reduced luminal spermatozoa in the epididymis. The seminal vesicles and prostate exhibited marked atrophy, decreased secretory material, epithelial hyperplasia, and necrosis, accompanied by significant organ weight reduction and diminished PCNA immunoexpression. Pretreatment with either PSE or GSE significantly ameliorated these CP-induced alterations, restored organ weights, and enhanced PCNA immunoexpression. Both PSE and GSE demonstrate promising p...
Featuring
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Histopathological and PCNA-Based Immunohistochemical Assessment of Pumpkin and Grape Seed Extracts in Protecting Male Rat Accessory Glands Under Chemotherapeutic Stress
Ibrahim Elmaghraby, Zeinab Said, Marwa Darweish, Doaa Galal El-Sahra and Ahmed Ibrahim El-Nemr
Advanced Analytical Pathology, Vol. 1, Pages. 12-21
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Assessing the Pathological Diagnostic Skills of Veterinary Students for Ovine Pulmonary Adenocarcinoma
Ibrahim Elmaghraby, Osama Ahmed, Shymaa Moustafa and Salma Shoulah
Advanced Analytical Pathology, Vol. 1, Pages. 1-11
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