Yasemin Bircan Yildirim* and Hediye Tugce Vurmay
...er aerogenes, Klebsiella pneumoniae, and Enterobacter cloacae were isolated from the gill samples, while E. aerogenes, E. cloacae, Proteus vulgaris, K. pneumoniae and Pseudomonas fluorescens were isolated from the fish intestine samples. E. aerogenes (68.4%), Serratia spp. (53.3%), E. cloacae (50%) and K. pneumoniae (44.4%) showed...
Muhammad Usman Qamar1, 2, Sidrah Saleem1, Usman Arshad1, Muhammad Farhan Rasheed3, Hasan Ejaz4, Naveed Shahzad5 and Shah Jahan6,*
... species; E. coli, K. pneumoniae, E. cloacae, A. baumannii and P. aeruginosa, were isolated and identified using VITEK 2 compact system. Carbapenamase and metallo-β-lactamase (MBL) production by these isolates was confirmed by modified Hodge’s test and disc potentiation method, respectively. The blaNDM-1genewas amplified by PCR and sequenced. MIC of different antibiotics were determined by VITEK 2 system and antibacte...
Hanif Ur Rahman1, Umer Saddique1, Zahoor-ul-Hassan1, Shakoor Ahmad1, Muhammad Kamal Shah1, Said Sajjad Ali Shah1, Farhan Anwar Khan1, Fazli Rabbani2, Muhammad Asif Hussain2, Attaur-Rahman2, Irshad Ahmad3,4 and Sadeeq ur Rahman2,*
...Contagious Caprine Pleuropneumonia (CCPP), a highly contagious and economically devastating disease, is caused by Mycoplasma mycoides (MM) - a cluster of six member species. However, literature is not available regarding the causative agents of CCPP in sheep and goats in Northern Pakistan. The current study was thus aimed to identify relative abundance of members of MM in small ruminants suspected of CCPP in Northern region of Pakistan. A total of 300 s...
Muhammad Sarfraz*, Shahida Hasnain
...n used to identify K. pneumoniae and E. coli; preliminary screening for AmpC beta-lactamase production was done by cefoxitin disc followed by inhibitor based confirmatory method. Antibiogram was performed following CLSI guidelines 2013. Of 11,725 clinical specimens, 29 % were culture-positive. 80% K. pneumoniae and 70% E. coli showed resistance to cefotaxime or ceftazidime. Only63% K...
Junaid Akhtar1,2, Sidrah Saleem1, Naveed Shahzad3, Abdul Waheed1, Iqra Jameel1, Farhan Rasheed4 and Shah Jahan5,*
...ginosa (n=26), K. pneumoniae (n=19), E. coli (n=16), C. ferundi (n=04), P. vulgaris (n=02) and E. cloacae (n=01), were isolated from clinical samples of hospitalized patients. Out of these 100 carbapenem resistant isolates, 93 and 89 isolates were positive for cabapenemase and β-lactamase production, respectively. Notably, 3 (3.3%) of MBL producing strains harbor blaIMP gene while 29 (32.5%) of MBL ...
Soumble Zulfiqar1, Khuram Shehzad1, Sana Tahir1, Khalid A. Al-Ghanim2 and Abdul Rauf Shakoori1,2,3,*
...y isolated Klebsiella pneumoniae KW strain. The CueO protein was purified to homogeneity by nickel affinity chromatography. Enzyme assays of CueO protein with phenolic substrates revealed its laccase activity. The kinetic studies showed Km value of 0.2µM, kKcat 0.68 S-1 and Kcat/km 1.2S-1 µM-1 for 2,6-Dimethoxyphenol (DMP) and Km value of 0.25mM, Kcat 300 S-1 and Kcat/Km=1200S-1mM
Muhammad Hidayat Rasool1,*, Muhammad Zaheer1, Muhammad Farooque Hassan2, Muhammad Shafique1 and Muhammad Usman Qamar1
...ency were; Klebsiella pneumoniae (59; 38.8%), Pseudomonas aeruginosa (46; 30.3%), Escherichia coli (36; 23.7%), Klebsiella oxytoca (4; 2.6%), Serratia marcescens (3; 2%), Enterobacter agglomerans (2; 1.3%) and Enterobacter cloacae (2; 1.3%). Out of 103 carbapenem-resistant isolates, 58 (55.1%) were positive by DDDT and 49 (46.7%) by MHT. Most of the carbapenems MBL resistant isolates were resistant towards drugs...
Sumreen Hayat1,6, Muhammad Hussnain Siddique2, Bilal Aslam1, Habibullah Nadeem2, Asma Ashraf3, Muhammad Saqalein1, Mohsin Khurshid4, Naveed Shahzad5 and Saima Muzammil1,*
...
...
Ali Mujtab Shah1,2,3, Muhammad Naeem2, Muhammad Giasuddin Shah2, Muhammad Haaroon2, Quanhui Peng1 and Zhisheng Wang1,*
...ps respectively, whereas pneumonia problem was observed as 20, 40 and 60% calves were affected accordingly. It is concluded from the present study theconcentration of serum IgG following administration of colostrum by ST method increases at a rate to that in calves fed by NS and NB and reaches a level consistent with high calf survival, it is also concluded from the study that the body weight gain is increased by ST feeding.
...
Abdul Waheed1,2, Sidrah Saleem1, Naveed Shahzad3, Junaid Akhtar4, Muhammad Saeed5, Iqra Jameel1, Farhan Rasheed6 and Shah Jahan7,*
.../i>(n=60), Klebsiella pneumoniae (n=20), Enterobacter cloaca (n=09), Enterobacter aerogenes (n=02), Proteus mirabilis (n=05), Proteus vulgaris (n=02) and Citrobacter freundii (n=02). Extended spectrum beta lactamases (ESBL) production was screened by double disc synergy test (DDST) and combination disc method as per Clinical Laboratory Standard Institute (CLSI) guidelines 2015. Presence of &ld...
Lin Sun1, Xiaofang Bu2, Jian Wang1*, Xiaorui Liu1 and Zhanyi Kong2
... injury of Mycoplasma pneumoniae pneumonia (MPP). However, the exact mechanism of CXCL8 and its mRNA in the children with Mycoplasmal pneumonia needs to be further clarified. The concentration of the CXCL8 in serum and the level of CXCL8 mRNA in PBMCs of forty-eight children were dynamically measured by ELISA and PCR. The ratio of lgcDNA/lgGAPDH was regarded as the extreme level of ...
Ahsan Anjum1, Asim Aslam1, Raheela Akhtar1, Tahir Yaqub2, Muti-ur-Rehman Khan1, Rizwana Sultan3, Saba Usman1, Aneela Zameer Durrani4 and Muhammad Usman4*
...>Contagious Bovine Pleuropneumonia (CBPP) is an infectious respiratory disease of cattle, characterized by anorexia, fever, dyspnea, polypnea, cough, and nasal discharges and caused by Mycoplasma mycoides subsp. mycoides. This study was carried out in abattoirs of Lahore, Kasur and Jhang districts of Punjab, Pakistan. Different tissue samples were collected from 560 cattle showing respiratory signs at slaughter. Lung samples were used for detecti...
Farzana Rashid1, Rabia Masood1 and Mariam Faiz2*
... prevalence of MBL in K. pneumoniae, P. aeruginosa, E. coli and Acinetobacter Species was 26%, 52%, 27% and 5% respectively. Prevalence of blaNDM-1 gene was 51% identified in 14 strains of P. aeruginosa, 2 strains of A. Baumanni, 29 strains of E. coli and 18 strains of K. pneumoniae. In conclusion, high prevalence of blaNDM-1 in our strains is a serious concern. A careful use of carbapenems is important to prevent the spread...
Asmaa A. Darwish
...rations related to sheep pneumonia, arthritis and enteritis and spot the light on MMP-2 and MMP-9 diagnostic and prognostic accuracy in these conditions. 80 barki lambs were divided into four groups control group (CG), pneumonic group (PG), arthritic group (AG) and enteric group (EG). Blood samples were collected from the four groups then haematologically, biochemically and statistically analyzed. A significant (P< 0.05) microcytic hypochromic anemia was ob...
Ambrin1*, Ghulam Dastagir1, Jehan Bakht2 and Muhammad Adil1
... epidermidis, Klebsiella pneumonia, Shigella flexneri and Staphylococcus aureus. Highest zone of inhibition against Klebsiella pneumonia and Pseudomonas aeruginosa was shown by ethyl acetate and ethanolic extracts of O. biennis. Lowest MIC value was noted for ethyl acetate and ethanolic extracts of O. biennis against Staphylococcus epidermidis, Staphylococcus aureus, Salmonella typhi an...
Md. Sahidur Rahman
Origin and Spillover of Coronaviruses: Prospects of One Health Action
...e recent one that showed pneumonia-like syndrome named COVID-19. It was assumed that SARS-CoV-2 was initially a spillover from bats due to its genomic similarity with Bat-CoV-RaTG13, where pangolins may have acted as an intermediate host. Wild trade, ecotourism, destruction of wild habitat, and other anthropogenic activities disrupt the human-wildlife barrier which leads to the spillover process. In these circumstances, a One Health approach is crucial to impl...
Mumtazur Rahman1, Farhan Anwar Khan1,*,Umar Sadique1, Ijaz Ahmad1, Shakoor Ahmad1, Faisal Ahmad1,2, Hayatullah Khan1,3, Muhammad Saeed1, Faiz Ur Rehman1, Ibrar Hussain1, M. Faraz Khan1, M. Izhar ul Haque1 and Hanif-ur-Rehman1,3
...Contagious caprine pleuropneumonia (CCPP) caused by Mycoplasma capricolum subsp. capripneumoniae (Mccp) is a highly contagious and deadly disease of small ruminants, especially goats. It is an economically important disease, causing high morbidity and mortality in goats. More recently Mccp Pakistan strain has been identified and isolated by our laboratory. Alarmingly, antimicrobials in animals are inappropriate...
Ayesha Alam1,* and Labeeb Ali2
...gestive tract disorders, pneumonia and conjunctivitis. Additionally, traces of COVID-19 were also found in sewage water sources in some countries like Italy bringing attention towards analysis of sewage water. Based on information from cited literature it is estimated that an individual handling sewage water have approximately 1% chance of becoming infected with virus. Treated sewage effluent and reuse of sewage water for recycle purposes must be considered as...
Khurram Goraya1,*, Qais ALRawahi1, Sultan ALBalushi1, Hani ALSaadi1, Sami ALRahbi1, Zahir ALAlawi1, Muhammad Hammad Hussain2 and Madad Hussain1
...ated deaths (n=10, 20%), pneumonia (n=8, 16%), old age (n=4, 8%), impaction of stomach caused by foreign bodies (n=3, 6%) and bloat (n=2, 4%). The exact cause of death could not be established in 5 (10%) mortalities. Internal organs were screened for the presence of adult worms. However, no parasite was observed in these animals. These findings demonstrated that more than 50% mortalities could be reduced by only controlling aggression-related injuries and taki...
Maimoona Imran1,2, Soumble Zulfiqar2, Hafsa Saeed2 and Farah Rauf Shakoori1*
... resistant Klebsiella pneumoniae strains KW and CC were investigated for their bioremediation potential. Maximum uptake of Cu++ by mid log phase cultures of KW and CC was 19.26 and 28.77µg per mg cell dry weight, respectively. The strains also exhibited efflux ability; KW possessed more efficient efflux system as it expelled 94.46% of maximum stored copper within 24 h as compared to 56% by CC. These strains were also found substantially...
Waqar Ali1,3*, Muhammad Yaqoob2, Abida Arshad1, Guifu Dai3,Safir Ullah Khan1, Muhammad Arshad Malik4, Imran Ullah1 and Ihsan Ullah1
...13.5%) and Klebsiella pneumonia (8.4%). Staphylococcus aureus was found most prevalent pathogen that causes mastitis in goats. The overall prevalence of goat mastitis was recorded (11.67%). Risk factor like age, lactation stage, parity number and unhygienic condition showed statistically significant association with the prevalence of mastitis in beetal goats. Antibiotics sensitivity profiles of investigated bacteria showed that these were mo...
Ou-Mei Hao1*, Xue-Feng Wang1, Zhi-Jun Yue1, Chun-Hong Nan1,Yan Yu1,Tong Zhang2,Yu-Feng Liu3 and Xue Zhao1
...tion after Mycoplasma pneumoniae pneumonia (MPP) infection and compare the therapeutic effects of Qing Zhi Zhi Ke formula (QZZKF), QZZKF combined with azithromycin (AZM) and AZM on experimental mice. Fifty female BALB/c mice were randomly divided into five groups: control, model, AZM (0.01 g/kg/day), QZZKF (10.16 g/kg/day), AZM (0.01 g/kg/day) combined with QZZKF (10.16 g/kg/day). QZZKF and AZM were administered by or...
Hailemariam Assefa1*, Tesfahun Dagnaw2
...erichia coli, Klebsiella pneumoniae, Staphylococcus aureus and Streptococcus pyogenes were used as tested bacterial species whereas Yeast, Mold and Dandruff were selected as tested fungi. The effect was seen high on Staphylococcus aureus, while the Klebsiella pneumoniae was less affected by any of the used extracts. Methanolic extract was more active than petroleum ether and ethyl acetate extract on all three tested fungal s...
G. Basana Gowda*, N.B. Patil, M. Sahu, S.R. Prabhukarthikeyan, S. Raghu, G.P. Pandi, T. Adak, C.K. Swain, S. Pokhare, S.D. Mohapatra and P.C. Rath
...s fusiformis, Klebsiella pneumonia (all four from resistant populations) and Achromobacter sp (from a susceptible population). Present study provides a basis for elucidating the role of the gut bacteria in the phosphine resistance and design novel strategies for the management of T. castaneum....
Sharjeel Saif1, Asghar Ali Kamboh1*, Ghulam Mustafa Solangi2, Rehana Burriro3, Hasina Baloch1, Atta Muhammad Memon2
... cultured on PPLO (pleuropneumonia like organism) media and were found negative for Mycoplasma spp. The prevalence of mycoplasma mastitis in this population of dairy buffaloes was recorded <0.23%. The study results suggested that the mycoplasma mastitis did not exist in buffaloes of study area, however, further studies using molecular tools are warranted to validate these findings.
Keywords | Dairy buffalo, M. bovis, Mastitis, Mycoplasma, Survey&nbs...
Roheela Yasmeen1* and Samia Chaudhry2
...ngoing outbreak of viral pneumonia. This pandemic started from Wuhan City, China and has spread throughout most parts of the world (210 countries). COVID-19 is a large sized, enveloped, positive stranded RNA virus. Out of the four known genera, alpha and beta corona viruses are the most commonly recognized viruses infecting human beings. COVID-19 is a new virus that is highly contagious. It spreads through infected persons in its prodromal stage which suggest ...
Mona M. Osman1, Kamelia M. Osman2, Manal Abu Elmakarem Mohamed1, Mahmoud E. Hashad2, Jeeser Alves Almeida3, Alaa Saad4, Octavio Luiz Franco5,6, Heba N. Deif 2*
...inini) and Mycoplasma ovipneumoniae (M. ovipneumoniae). It shed light on the current knowledge gap in the role of two potential virulence determinants viz H2S production and catalase activity, for their possible roles in biofilm formation, antibiotic resistance and hemolytic activity of M. ovipneumoniae. The recovered sheep isolates of M. arginini and M. ovipneumon...
Basanta Kumar Adhikari1*, Deepak Subedi1*, Sumit Jyoti1, Krishna Kaphle2, Chet Narayan Kharel3, Doj Raj Khanal4
...Contagious Caprine Pleuropneumonia (CCPP) in the Rupandehi and Palpa districts of Nepal. A total of 89 goat serum samples were tested by using a competitive enzyme-linked immunosorbent assay (c-ELISA) for the presence of antibodies against Mycoplasma capricolum capripneumoniae. Out of the total serum sample tested, 3 were seropositive for CCPP giving an overall apparent seroprevalence of 3.37% and true seroprevalence of 3.4%...
Sri Melia1*, Indri Juliyarsi1, Yulianti Fitri Kurnia1, Evy Rossi2, Hurriya Alzahra3
...a inokua, and Klebsiella pneumonia; and (3) Molecular identification of LAB by analysis of the base sequence of the 16S RNA gene. The whey for this study was collected in Lasi Farm in Agam Regency, West Sumatra, Indonesia. The data was subsequently subJected to descriptive analysis. The bacteria obtained were rod-shaped that were catalase negative and homofermentative. Lactic acid bacteria were found to have antibacterial activity against pathogenic bacteria s...
Hui Xu1,*, Ronghua Luo1, Yaping Duan1, Mingjia Chen1, Jiaxu Zhou1 and Xiaoli Shao2
...children with Mycoplasma pneumoniae pneumonia (MPP). From January 2018 to January 2020, 114 children with MPP in our hospital were selected and randomly divided into control group and experimental group, each containing 57 cases. The control group was given azithromycin. The experimental group was given fibrobronchoscope-assisted lavage on the basis of the control group. The curative effect, the time of remission of clinical...
Ochieng Onunga George*, Owuor Ndonga Millicent Florence, Mario Kollenberg
... coli, P. aeruginosa, K. pneumoniae, E. faecalis, and S. dysenteriae. Bacterial concentrations cfu/100ml of abattoir effluents ranged from 3.17 × 106 of TC. 3.94x104 of FC, 2.84x104 of E. faecalis, 8.65x104 of E. coli, and BOD of 828.04mg/l.Fungi isolated were., Aspergillus niger, Fusarium oxysporum, Penicillium spp, Aspergillus fumigatus, Saccharomyces cerevisiae, Aspergillus flavus. Parasites isolated from samples of effluent and water were Balantidium...
Sadaf Ansari1, Shahid Hussain Abro1, Abdul Jabbar Tanweer2, Abdullah Sethar3, Ghulam Abbas4, Sabahat Ansari1, Asghar Ali Kamboh1*
...10% and 10%), Klebsiella pneumoniae (15%, 10% and 0%) and Shigella dysenteriae (10%, 12.5% and 5%) in beef, mutton and fish respectively. While from cooked beef, mutton and fish the prevalence of E. coli (25%, 25% and 20%), S. aureus (15%, 15% and 10%), S. enteritidis and B. cereus (10%, 10% and 10%) were observed. The highest (p < 0.05) bacterial load (g¹) was detected in raw (1.76×106) and cooked (6.1×104) beef than raw (1.55×...
Mehwish Saleem1* and Amina Atiq2
...ies (E. coli, Klebsiella pneumonia, Bacillus subtilis and Staphylococcus aureus). Additionally, Staphylococcus aureus showed the greatest sensitivity to garlic nanoparticles, with inhibitory zones of 19 mm, 17 mm, and 18 mm for an aqueous extract for aqueous extract and for ethanolic garlic extract was (19mm, 17mm and 15mm) K. pneumonia was the least susceptible, with inhibitory zones for aqueous and ethanolic garlic extract...
S. Feroza 1, A. G. Arijo1†, F. M. Bilqees2* and M. S. Phulan1
...vere congestion, chronic pneumonia, exuded in one lobe of the lung were seen, whereas, necrosis and atrophy in infected heart were notable observations....
Nuzhat Munawar1, Abrar Hussain1, Imran Sajid2, Zahra Noreen1, Muhammad Aslam3*, Zeeshan Rehman1 and Aamir Ali3,4
...eems inactive against K. pneumoniae as compared to control. When tested on S. aureus, chloroform showed a 10 mm inhibition zone while it was inactive against all other experienced bacteria. Ethyl acetate extract gave an inhibition zone of 10 mm against S. aureus, B. subtilis, and P. aeruginosa while it was inactive against the rest of the bacteria. Using disc diffusion assay, ethyl acetate extract gave 12 mm, 8mm, and 7 mm zone of inhibition against B. subtili...
Safika1*, Ni Luh Putu Ika Mayasari1, Juliadi Ramadhan2
...resistance in Klebsiella pneumoniae isolated from clinical cats in Bogor, Indonesia. This study’s total sample comprised of 58 clinical cat laryngeal swabs. Samples were isolated and biochemically and molecularly identified. The Kirby-Bauer disk diffusion method was used to test positive isolates, followed by PCR method to discover the resistance coding genes. The results showed that ampicillin showed 76.0% resistance to Klebsiella ...
Ghada A. Ibrahim1*, Mohamed Sayed Helal1, Nahed Abd Elhafeeze Kamoura2, Mohamed Samir3,4, Amira Mohamed Mazid5, Mohamed F.M. Farag6
... a high proportion of K. pneumoniae (18.7%). In-vitro antibiogram exhibited a wide MDR phenotype. The virulence genes (fimA, aerobactin, rmpA and magA) were exhibited in 90%, 85%, 90% and 80% of the tested isolates, respectively. Moreover, ESBL-resistant markers (blaCTX-M, blaTEM and blaSHV) were found in 100%, 85% and 80% of the same isolates, respectively. A significant positive correlation (R = 0.7) was reported between aerobactine and magA virulence genes ...
Ghulam Mustafa Solangi4, Zaheer Ahmed Nizamani1*, Mansoor Tariq1, Zubair Ahmed Leghari2, Asghar Ali Kamboh3, Barirah Rehman Talpur1
...Contagious Caprine Pleuropneumonia (CCPP) is a serious and economically important respiratory disease which causes significant losses in goat population throughout Asia, Middle East, Europe and Africa. In order to investigate the seroprevalence of CCPP in goat population of Sindh province, a total of 800 serum samples from four districts of Sindh (Thatta, Tharparkar, Jamshoro and Larkana) representing four agro-ecological zones of Sindh were examined by using ...
Fathin Faahimaah Abdul Hamid1, Mohd Farhan Hanif Reduan1*, Jasni Sabri1 , Faez Firdaus Jesse Abdullah2,Mohammed Naji Odhah1, Nur Athirah Binti Abdul Manaf1, Mohd Jefri Norsidin2 , Siti Nor Che Yahya1, Intan Noor Aina Kamaruzaman1, Nur Zul Izzati Mohd Rajdi1
... of the common causes of pneumonia caused by Mannheimia haemolytica. Evaluation of the hematological and serum biochemistry changes is critical to determine the effectiveness of the treatment approach in reducing the severity of infection. Healthy goats (n=20) were equally divided into 5 groups: Mannheimia haemolytica 107 concentration was inoculated intranasally to all goats except goats of Group 1 which served as the negative control, Group 2 was the positiv...
Rafik Soliman1*, Rafik H. Sayed2, Hanan Mahmoud3, Heidy Abo El-Yazeed1, Marsell Saad1, Shaimaa Abdelall Elsaady2, Khalid Sh3
...eris (7.3% and 2.5%), K. pneumoniae (4.4% and 3.3%), Str. fecalis (4.4% and 0%), Str. pyogenes (2.9% and 0%) and Pseudomonas aeruginosa (1.5% and 3.3%). Among the recovered E. coli serotypes, the serotype O111:K58 (B4) was the most prevalent (38.2%). An inactivated polyvalent autogenous mastitis vaccine was prepared from the most encountered bacterial pathogens, namely, E. coli, S. aureus, Str. agalactiae and Str. dysgalactiae. The selected strains for vaccine...
Yue-Xia Ding1, Qun Wu2, Zhao Namula1 and Yi Ma1,*
...ducted for Streptococcus pneumonia ATCC49619 and erythromycin-sensitive strains by gradually increasing the antimicrobial concentration. Plasmid conjugation test was carried out by membrane filtration method. The correlation between antimicrobial resistance and virulence traits was analyzed by LD50 and related genes. Sensitive Streptococcus isolates to erythromycin and S. pneumoniae ATCC49619 were induced to resistance in vi...
Samin Ullah1, Huma Rizwana1, Abdul Kabir1,2*, Atique Ahmed Behan1, Muhammad Naeem1, Ghulam Shabbir Barham1, Abdul Hafeez Bukero1, Anees ur Rahman1, Naseeb Ullah1 and Shafiq ur Rahman Shah1
...ues such as diarrhea and pneumonia. The findings suggest that providing 4 liters of colostrum to crossbred Holstein Friesian calves within 30 minutes of birth can improve their immunity and health. This study provides evidence for farmers to consider increasing colostrum feeding quantity in their management practices to improve the health and growth performance of their calves. Information aids farmers raising crossbred Holstein Friesian calves; cut vet expens...
Iram Liaqat1*, Noor Muhammad1, Muhammad Mubin2, Najma Arshad3, Tehreema Iftikhar4, Sumera Sajjad5 and Farzana Rashid5
...erichia coli, Klebsiella pneumoniae, Bacillus licheniformis, Pseudomonas aeruginosa and Bacillus subtilis). Secondary screening using ethyl acetate extracts also showed significant ZI in the range of 06 – 14.0 mm (P ≤ 0.05) against these pathogens thus confirming their bioactive potential. The minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) of screened Actinomycetes was in the range of 1.3 - 3.5 mgmL-1 and 1.9 &ndas...
Jameel-ur-Rehman1, Asghar Ali Kamboh1*, Ahmad Ali Moryani1, Riaz Ahmed Leghari1, Abdullah Sethar2, Ali Raza Nizamani3, Abdul Ahad Soomro3, Kanwar Kumar Malhi1
...& 18.3%), Klebsiella pneumoniae (15% & 13.3%), Staphylococcus epidermidis (13.3% & 10%), Klebsiella oxytoca (10% & 8.3%), and Streptococcus pyogenes (6.6% & 1.6%) in market and pond fish samples respectively. According to antibiotic susceptibility tests, among 11 tested antimicrobials, S. agalactiae and S. pyogenes were found resistant to 9 antibiotics; followed by P. aeruginosa and P. flavescens 8; S. flexneri 7; K. oxytoca and S. epiderm...
Orooba Mohammed Saeed Ibrahim1*, Rawaa Saladdin Jumaa2, Nibras Zeyad Yahya1
...us aureus, Sterptococcus pneumoniae and Klebsiella pneumonia. The antibacterial activity of fruit juice extract on bacterial strain was determined using macro-broth dilution, agar well diffusion methods and time kill curve. Results revealed that the used juice extracts were variously bacteriostatic and bactericidal against the examined bacteria depending on concentration. Phytochemicals analysis indicated the presence of al...
Abdul Latif Rind1*, Shahid Hussain Abro1, Rameez Raja Kaleri2,3, Ghulam Mustafa Solangi4, Raza Ali Mangi5, Muhammad Anees Memon6, Depeesh Kumar Bhuptani7, Sana Noor8, Zainab Lanjar9, Zahid Ali Mangrio10 and Abdul Wahid Solangi1
...arrhea, coughing, fever, pneumonia, stomatitis, and occulo-nasal discharge. Furthermore, no instances of pregnancy were reported during the research. These findings underscore the high prevalence of PPR in sheep and goats within District Sanghar, emphasizing the urgent requirement for effective control measures to curb its propagation....
Nguyen Thi Thu Hien1, Dong Huu Rin2, Nguyen Xuan Hoa1, Le Viet Tuan Khanh2, Phung Thang Long1*, Dinh Thi Bich Lan1
... (432 bp) encoding M. hyopneumoniae P36 protein, express this protein in E. coli BL21(DE3), and evaluate its antigenicity as a base for further studies to against M. hyopneumoniae. The gene encoding M. hyopneumoniae P36 protein isolated from DNA of fresh lung tissue samples from PEP infected pigs was cloned into pGEM®-T Easy vector for sequencing, and then was inserted into pET28a vect...
Hayder M. Watban1,2, Nabeel M.H. Al-Maaly2*
...l infections. Klebsiella pneumoniae is one of the bacterial diseases that infect camels and induce pneumonia. In this study, one hundred camels were examined and samples were collected from lungs of pneumonic camels slaughtered in Al-Muthanna abattoir. Swabs were taken from lungs of slaughter camels, and cultured on blood and MacConkey agars. The bacterial growth was subjected into gram stain and subculture to Klebsiella
Yunxia Guo1, Xia Zhou1*, Xin Huang2 and Fagang Zhong2
...at bronchitis and bronchopneumonia were commonly occurring respiratory diseases in cattle. The highest incidence of bacterial organism Staphylococcus aureus (83.6%), followed by Streptococcus pneumoniae (80.3%) and Klebsiella pneumoniae (77.0%) were recorded in the respiratory tract samples. Pasteurella multocida, Bacillus obstructivus and Mycoplasma alkalescens exhibited 100% resistance a...
Hafsa Saeed, Soumble Zulfiqar, Abeedha Tu-Allah Khan and Abdul Rauf Shakoori*
... yet been explored in K. pneumoniae. This study focused on molecular characterization of ZntR, a transcriptional regulator of znt operon. The minimum inhibitory concentration of zinc, lead and cadmium were 10, 10 and 4 mM, respectively against K. pneumoniae KW. The strain also exhibited metal uptake and storage ability. Having amplified and cloned zntR gene, expressed and purified ZntR protein showed binding with zntA promot...
Amany M. Reyad1*, Aya Maher Rabie1, Reda Mohamed Taha1 and Khalid El-Dougdoug2
...gn: justify;">Klebsiella pneumoniae were isolated and mixed in a cocktail, then applied for the treatment of the collected meat samples. At room temperature (24°C), the number falls over time until reaching 72 hours for all bacterial species. The results obtained showed a significant reduction in the bacterial count of
Eman, M. Abd El-Mottaleb.*; Ahmed, S.A."; Maysa, H.M.*; Salem, S.A.* and M. M. El-Shenawy
... as well as interstitial pneumonia and proliferation of the cells lining the bronchioles. Severe edema and infiltration of mononuclear inflammatory cells with proliferative fibrous connective tissues were seen in the heart. Liver showed severe edema, fibrous connective tissues proliferation, various sized organized thrombus with severe infiltration by mononuclear inflammatory cells. Depletion of the white pulp of the spleen as well as edema in the lymph node. ...
Nurhashunatil Mar’ah1, Safika Safika2*, Agustin Indrawati2
...ce factors of Klebsiella pneumoniae from Dairy Farm isolates in South Sulawesi, Indonesia. Twelve isolates obtained in the field were tested for phenotype resistance profile with four antibiotics of β-lactams using the Kirby-Bauer method. The Polymerase Chain Reaction (PCR) was used to detect the resistance genes and virulence factors of K. pneumoniae. Samples isolated from water sources showed a resistance profile to c...
IRFANA IQBAL1*, PAKEEZA TANWEER1, FARKHANDA MANZOOR1, MUHAMMAD NAUMAN AFTAB2, AFSHAN KALEEM3, ROHEENA ABDULLAH3, ASMA ZAFAR4 & MEHWISH IQTEDAR3
.... aeruginosa, E.coli, K. pneumoniae and S. aureus. The original bacterial inoculum was serially diluted (adjusted to 1.5 X 106 CFU) and spread on the nutrient agar plates. Whattmann filter paper discs were soaked in three different concentrations (50%, 70% & 90%) of each of the three unifloral honey samples for 48 hrs. The filter discs were placed on the agar plates seeded with the individual bacteria. Solitary effect of antibiotic discs (Ciprofloxacin, Im...
Ahmad Shandookh Hameed1, Ali Abd Kadhum2 and Murtada Wafi Beden1*
...th ventilator associated pneumonia of whom 23 were confirmed positive. for SARS-CoV-2 (infectious group) and 18 were tested negative for SARS-CoV-2 (non-infectious), were recruited in this study during the period from February to September 2022 at Al-Hussein. Teaching Hospital in Thi-Qar province, Iraq. Endotracheal aspirate samples were collected for the presence of microbes, using traditional cultivation methods along with antibiotic susceptibility testing f...
Ali Abd Kadhum
...eruginosa, Streptococcus pneumonia, Moraxilla catarrhalis, and Klebsiella pneumonia resistance (100%) to the antibiotic Amoxycillin. While isolates of the bacterial species Staphulococcu aureu, Escherichia coli, Pseudomonas aeruginosa, Klebsiella pneumonia, and Proteus vulgaris showed resistance (100%) to the antibiotic azithromycin. Klebsiella pneumonia...
Maimoona Imran2, Farah Rauf Shakoori2*, Soumble Zulfiqar1,
Abeedha Tu-Allah Khan1 and Abdul Rauf Shakoori1*
...terial cells. Klebsiella pneumoniae remains a major health concern around the globe due to its associated infections, such as, pneumonia, meningitis and bloodstream, surgical site and wound infections. The present study reports a locally isolated K. pneumoniae strain exhibiting high resistance (MIC=90mM) against Ag+ and its accumulation potential. Transcription of Cus (Cu sensing) determin...
Mohamed E. Enany1, Ahmed M. Hamouda2, Reem M. Khashaba3*
...phimurium and Klebsiella pneumoniae. Among the plant extracts used, cloves showed a broad antimicrobial spectrum three examined strains with inhibition zones between 17-19 mm followed by garlic with inhibition zones between 16-18 mm and rosemary with 8-13mm. Meanwhile Ficus sycomorus and Ziziphus spina-christi have no effect against the three tested strains. Combinations of these extracts and antibiotics (amoxicillin, doxycycline and florophenicol) were applie...
Hayder A.H. Al-Mutar*, Baqer J. Hasan, Khawla Abbas Hussein, Souhayla Oneeis Hussain
... lutetiensis, Klebsiells pneumoniae, Escherichia coli and Staphylococcus aurreus were present in a percentage from 94.61%, 93.25%, 80.5% and 78.69%. respectively. The study concluded that there are many types of Saprophytic bacterial proliferation during estrus cycle inside vagina and uterus of cows with a variation of different countries, bread and age.
Keywords | Saprophytic bacteria, Estrus cycle, Cows, PCR, Clinical isolation...
Mohammed A. Hamad1*, Murtadha Abdule Mahdi Al-Mudhafar2, Alaa Fayeq Habeeb3, Firas R. Jameel1, Ismael Raheem Al-Muhana2, Najemaddin A. Hamad5
...-align: justify;">Bovine pneumonia is a complex disease which results in significant financial losses. The aims of this investigation were to identify the respiratory syncytial virus, the prevalence of infection that has spread throughout Al-Anbar region, and the factors that contributed to the infection in Calves also by detecting the virus using a molecular technique. In this study, fifty-two (n=52) nasal swabs were collected from male and females in young c...
Soumble Zulfiqar* and Abdul Rauf Shakoori
...per-resistant Klebsiella pneumoniae strain were explored, which form a two-component regulatory system crucial for copper detoxification. The genes were successfully amplified and cloned into a vector. Their sequences were obtained through commercial services. The corresponding protein sequences were deduced and a comprehensive analysis was conducted, including multiple sequence alignments and phylogenetic tree construction. The findings reveal that CusS and C...
Hongxiao Li1*, Xiying Wu2, Dengxiang Wu1 and Jiangqing Yu1
...udomonas aeruginosa (Pa) pneumonia in elderly individuals. The sample of 50 elderly patients with PA pneumonia were selected and assigned into control group (Ctrl group, n=25, PPC/TS treatment) and experimental group (Exp group, n=25, amikacin +PPC/TS treatment) according to the single and even numbers. The between-group differences in CURB-65 score, CPIS score, APACHE II score, SOFA score, infection-related indicators, and ...
Binod G.C. 1 Nim Raj Sapkota 2 Binod Rayamajhee 2 Jayram Lamichhane 1 Pramod Poudel 1 Sunil Lekhak 3 Shovana Thapa 4 Santosh Khanal 1
Volume 2, Issue 5 September and October 2018 Pages 65-74
...chia coli and Klebsiella pneumoniae isolates, confirmed through using Modified Hodge Test (MHT). During the six months of this study (November, 2016 – April, 2017), a total of 1503 clinical samples including urine, blood, wound swabs and Cerebrospinal fluid (CSF) were collected and processed at Microbiology Laboratory, International Friendship Children’s Hospital, Kathmandu, Nepal. Among the E. coli and K. pneumonia<...
Eze E.M. 1 Unegbu V.N. 2 Ezebialu C.U. 3 Nneji I.R. 4
Volume 2, Issue 6 November and December 2018 Pages 147-155
...enes 15 (5%), Klebsiella pneumonia 55 (18.3%), Proteus mirabilis 25 (8.3 %), Pseudomonas aeruginosa 64 (21.3%), Neisseria gonorrhoeae 62 (20.7%), Candida albicans 56 (18.7%), and Trichomonas vaginalis 88 (29.3%), respectively. Frequency of occurrence was predominant with the age groups of 21-30 and 31-40 years; conversely was least in ages of 10-20 and those age >51 years; respectively. There was significant statistical difference between microbial infectio...
Fatiha Malki1*; Abdelkader Touati1
Novel Research in Microbiology Journal (2019), 3(1): 281-285
...-size: 11pt;">Klebsiella pneumoniae, Salmonella enteric), and three fungi (Aspergillus carbonarius, Mucor ramannianus, Candida albicans
Hafsa Zaib1; Rabia Kanwar1; Nishat Zafar1*; Sultan Ali1
Novel Research in Microbiology Journal (2019), 3(6): 526-534
...s aeruginosa, Klebsiella pneumonia and Escherichia coli. These pure cultures were then tested for resistance to different antibiotics using the Kirby–Bauer method. The prevalence of Gram positive bacteria (60.83%) was more than that of Gram negative bacteria (24.13%). The results of antibiotics susceptibility tests were analyzed statistically....
Bassma H. Elwakil1; Safaa M. Ali2*; Soad F. Hafez3; Adnan A. Bekhit4; Moustafa Y. El-Naggar5; Zakia A. Olama5
Novel Research in Microbiology Journal (2019), 3(6): 535-545
...nbsp;
Klebsiella pneumoniae has been identified as an opportunistic pathogen associated with both community- acquired and nosocomial infections mainly among patients admitted to the Intensive care units (ICUs). Some resistant genes were transferred vertically or horizontally within many microbial communities, directly between bacteria through plasmids or integrin. Different techniques including; phenotypic and genetic ones were used to evaluate the pre...
Faisal Ahmad1,2,3, Farhan Anwar Khan1, Hayatullah Khan4* and Muhammad Saeed1
...ontagious caprine pleuro-pneumonia (CCPP) is a fatal disease of goats and causes enormous economic losses due to high morbidity and mortality. CCPP is enlisted as a notifiable animal disease by the World Organisation for Animal Health. The causative agent of CCPP is Mycoplasma capricolum subsp. capripneumoniae (Mccp). The present study aimed to investigate the seroprevalence of CCPP in north-western Pakistan. The study areas...
Wafa H. Alamshani*; Faisal Al-Sarraj; Mashail A. Algamdi
...chia coli and Klebsiella pneumonia, making treatment more difficult.
Recurrent UTIs can be effectively treated with long-term antibiotics; however, they can have
several adverse side effects, and sometimes they may generate antibiotic-resistant strains. Due
to these downsides, alternative remedies based on plant extracts are increasingly being
considered for the prevention and treatment of urinary tract infection...
Enas M. Hefzy1*; Reda M. Taha2; Safaa Abd El Salam2; Abdelrhman Abdelmoktader1; Mahmoud A.F. Khalil3
...ns induced by Klebsiella pneumoniae (K. pneumoniae) include
pneumonia; urinary tract infections, liver abscesses, bacteremia, and others. The introduction
and spread of the hypervirulent K. pneumoniae (hvKp) strains have raised the number of
persons who are already susceptible to infections, including those who are hea...
Ramesh Bhandari*; Fenyong Sun; Qiuhui Pan
Novel Research in Microbiology Journal (2020), 4(3): 746-765
...hat may extend to severe pneumonia. Currently, there are no any vaccines or antiviral drugs against this virus. Treatment of patients is based mainly on symptomatic management. The aims of the present study were to summarize the information on the origin, evolution, structure and genomes, epidemiology, molecular immunopathogenesis, and diagnostic approaches of the SARS-CoV-2 coronavirus. Moreover, we discuss the current approaches, progress in vaccine developm...
Shimaa, A. Abdel Salam1; Raghda, Hager2*
Novel Research in Microbiology Journal (2020), 4(5): 968-978
...lood samples. Klebsiella pneumoniae (K. pneumoniae) was the most common isolated bacterium; that was represented by 24 isolates (68%). Out of the 35 GNB, only five isolates (14.3 %) were resistant to colistin by E-test, with MIC >256 𝜇g/ ml. The mcr-1 gene was detected only in one Pseudomonas aeruginosa (P. aeruginosa) isolate. This study concluded the low frequency of mcr-1 gene among the current GNB isolates. However...
Salwa M. Masoud1; Hend A. Refat2; Nada S. Sayed2; Mohamed K. Abd-El Aal2; Ahmed A. Dosocky2; Zeinab S. Mohammed3; Mohamed A. H. Abdel Wahab3; Dina M. Mekawy3; Ali I. Ali4; Basma A. Atya4; Katren T. Welliam4; Rehab M. Abd El-Baky1, 5; Zeinab S. Hashem1*
Novel Research in Microbiology Journal (2020), 4(5): 1005-1014
...ivity against Klebsiella pneumoniae ATCC10031 and Staphylococcus aureus ATCC 6538. This study revealed that bacteriophages could act as effective alternatives of antibiotics especially against multidrug resistant bacteria; however, further in-vivo and shelf stability studies are needed....
Bijay Kumar Shrestha*; Jenish Shakya; Manita Tumbahangphe; Bidhya Dhungana; Romika Shrestha; Jyoti Limbu
Novel Research in Microbiology Journal (2020), 4(6): 1015-1028
..., which is manifested as pneumonia clinically characterized by; fever, dry cough, shortness of breath and fatigue. Real time-Polymerase chain reaction (RT- PCR) and Radiological methods such as Computerized Tomography of chest (CT-scan) are the most preferred diagnostic tools. In fact, the CT-scan of chest is considered to be most sensitive, accurate and a rapid diagnostic tool to remove false negative results, and hence stands to be an efficient diagnostic to...
Yusuf Muhammed; Mkpouto Pius; Bashiru Sani; Bulus Timothy; Jafar Usman; Musa Garba
Novel Research in Microbiology Journal (2021), 5(1): 1063-1076
...r 2019, cases of unknown pneumonia-like disease connected to food markets were reported in China. The causative agent was identified as a Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV- 2), and the disease which spread all over the world, was named COVID-19. This pandemic has negatively affected quality of life and economy worldwide, thus motivating thoughtful search for treatment and prevention strategies. Efforts have been made in drug repositioni...
Bala, N. Umar1*; Jibril, Adamu1; Muhammad, T. Ahmad2; Kabiru, H. Ahmad3; Ochuko, Orakpoghenor4; Bashir, S. Aliyu5; Nuhu, Mohammed6; Aliyu Sada1,7
Novel Research in Microbiology Journal (2021), 5(2): 1162-1175
...lungs; leading to severe pneumonia, extensive ARDS and finally to death. Despite of this, the protective and pathogenic aspects of the human immunity have not been fully elucidated. Recent attempts conducted by several published research works have focused on information derived from the immune responses to the severe acute respiratory syndrome-related coronavirus diseases (mainly; SARS and MERS). However, these works lack sufficiency due to variations in the ...
Sara M. Farag1; Rasha A. Nasr1; Nesma G. El Sheikh2; Mona A. Khattab1*
Novel Research in Microbiology Journal (2021), 5(4): 1325-1337
... patients, as well as in pneumonia. This study aimed to investigate the potential utility of PSP as a predictive indicator of disease severity in COVID-19 patients. A total of 42 COVID-19 patients were enrolled in this study and stratified into moderate and severe groups, in addition to 15 healthy patients as controls. The PSP levels were measured using Enzyme Linked Immuno-Sorbent Assay (ELISA) within 24 h (1 day) as well as on the 5th day of admission to the...
Noura El-Kattan1*; Kamilia A.M. Allam2
Novel Research in Microbiology Journal (2021), 5(6): 1447-1462
...osa (25.2 %), Klebsiella pneumonia (23.5 %), Acinetobacter baumannii (12.2 %), Proteus vulgaris (7.8 %), and the less common isolate of Escherichia coli (5.2 %). Among 14 antibiotics tested in vitro for their susceptibility using the standard disk diffusion assay, results showed that imipenem was the most efficient antibiotic against most of the tested Gram (-) and Gram (+) isolates followed by meropenem. Currently, all the recovered bacterial isolates were MD...
Fatma Ahmed Abdel Aziz1; Gamal Fadl Mahmoud Gad1; Ahmed Mohamed Kamal El Shafei2; Reham Ali Ibrahem1*
Novel Research in Microbiology Journal (2022), 6(5): 1725-1741
...oteus sp., Streptococcus pneumonia, Klebsiella sp., and Haemophilus influenzae, were the least detected bacterial spp. Most of the bacterial isolates tested in this study exhibited high resistance to Amoxacillin-Clavulanic, Sulfamethoxazole-trimethoprim, Cefotaxime, and Cefoperazone. Using Cefoxitin, results of the phenotypic test predicted that 40.9 % of the Staphylococcal spp. were MRSA, and 23.6 % were MR-CoNS. The Polymerase chain reaction (PCR) technique ...
Apoorva Jain1; Ajay Kumar Oli1*; Sughosh Kulkarni2; Raghavendra D. Kulkarni2; Kelmani Chandrakanth3
Novel Research in Microbiology Journal (2024), 8(3): 2435-2451
...occus aureus, Klebsiella pneumoniae, Acinetobacter baumannii, Pseudomonas aeruginosa, and Enterobacter spp.) bacterial pathogens presents a formidable clinical challenge globally, affecting both the developed and developing nations. These pathogens, which are distinguished by their robust antibiotic resistance mechanisms, pose a significant threat to the public health. Their ability to evade traditional antimicrobial treatments underscores the urgent need for ...
Zine El Abidine Bzazou El Ouazzani1; Laila Reklaoui1; Houda Benaicha1,2; Said Barrijal1*
Novel Research in Microbiology Journal (2024), 8(4): 2491-2509
...chia coli and Klebsiella pneumonia; along with other Gram-negative bacteria (GNB), are known for their resilience to a broad spectrum of antibiotics, including last-resort options such as colistin, which is regarded as the last line of defense in treating multidrug- resistant (MDR) infections. This elevated level of resistance presents a substantial threat to the healthcare system, paving the way for a post-antibiotic era. In the present study, a checkerboard ...
Sarah Sonbol1; Nahla A. Hussein2*
Novel Research in Microbiology Journal (2024), 8(5): 2653-2665
..., and 52 % in Klebsiella pneumoniae) and the highest percentage of strain- specific genes (31 % versus 18 % in S. enterica and 24 % in K. pneumoniae). All bacterial strains included in the analysis encoded genes for resistance to commonly used antibacterials, including cell envelope acting antibiotics, aminoglycosides, quinolones, and sulphonamide. The resistance genes showed different distributions among the core, dispensab...
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