Qin Zhao1,2, Yani Sun1,2, Gaiping Zhang1,3, Frederik Widen4, En-Min Zhou1,2,*
...d is responsible for the induction of the protective humoral immune response. Six antigenic domains in the capsid protein have been identified. The fragmented proteins in these domains are analyzed for their application in serological diagnosis of HEV....
Muhammad Zubair, Sajid Mahmood Sajid
...in puberty and ovulation induction. The beneficial effects are manifested in male in terms of improvement of semen....
Zamarud Shah, Safdar Hussain Shah, Asad Jan and Gul Shad Ali
...lectrolyte leakage after induction of heat stress at 42°C compared to wild type plants (WT). No significant decrease was recorded in the chlorophyll content of overexpression lines whereas significant decrease was recorded in the chlorophyll content of WT plants following heat stress. Similarly, no significant reduction in the water content of the leaves of overexpression lines was recorded compared to 33.87% reduction in leaf water content of WT plants as...
Gökhan Kuş1,*, Hatice Mehtap Kutlu2, Djanan Vejselova2 and Emre Comlekci3
1Bikash Ranjan Ray and 2Mrinal Kanti Dasgupta
Management of root holoparasite Aeginetia pedunculata of (Orobanchaceae), causing wilt of sugarcane by trap and catch crops
...the basis of germination induction test of A. pedunculata seeds on root exudates as compared to that of three germination inducing chemicals (viz.; NaOCl, TTC (2,3,5-triphenyl tetrazolium chloride) and GR24-a strigol analogue) and growth of the parasite on excised root pieces of prevalent crop cultivars including sugarcane, were evaluated in A. pedunculata infested sugarcane field for their (crops) efficacy to deplete or recharge the parasite seeds in soil. Ri...
Tiansen Li1, Meiling Huang2, Zhen Wang1,Fei Guo3,Hui Zhang1,* and Chuangfu Chen1,*
...me fusion, and apoptosis induction, as assessed in appropriate in vitro (cell culture) and animal models. Results showed the omp10 mutant was dramatically attenuated for survival in macrophages and mice than the parent strain 2308. With decrease in the spleen/body weight ratios of mice infected with omp10 mutants were noted, inhibition of phagosome-Lysosome fusion was dramaticly impaired, and profound increase of apoptosis rate after expos...
Furqan Sabir1, Muhammad Tayyab1,*, Bushra Muneer2, Abu Saeed Hashmi1, Ali Raza Awan1, Naeem Rashid3, Muhammad Wasim1 and Sehrish Firyal1
....4 mM IPTG with the post induction time of 6 hours. PHYTN showed maximal activity at 80°C in 50 mM sodium acetate buffer pH 6. Presence of Fe3+ or Cu2+ showed an enhancing effect on the PHYTN activity. Thermostability studies demonstrated that PHYTN retains 88% residual activity when the protein was incubated at 80°C for 1.5 h in the presence of 1.5 mM Fe3+. The enzyme exhibited K
Zaheer Abbas*, Shaukat Ali, Jalal-Ud-Din and Ghulam M. Ali*
... primary calli on callus induction medium containing both 0.5mgL-1 2, 4-D and 1 mgL-1 picloram. Eighty percent of embryogenic calli were obtained on MS medium containing only single auxin while combination of auxin and cytokine in MS and Chu’s N6 media produced 53.33 and 35.56% embryogenic calli, respectively. Inclusion of 5 mgL-1 silver nitrate in embryogenic medium enhanced the production of type II embryogenic calli by 54.90% compared to control treat...
Müge Aliye Hekimoğlu*, Cüneyt Süzer,Şahin Saka andKürşat Firat
...uarium fish species. The induction and recovery times were determined at five stages of melanistic and not melanistic clownfish (Amphiprion ocellaris) and different sexes of Swordfish (Xiphophorus helleri). Clownfish were tested with 0.4, 0.6 and 0.8 ml/l phenoxyethanol and 0.5, 1 and 1.5 ml/l clove oil. Swordfish were tested with 1, 3 and 5 ml/l phenoxyethanol and 2, 4, 6 ml/l clove oil. For short-term application the safe dose for clownfish was...
Faiza Ambreen*1 and Muhammad Javed2
...n fish erythrocytes with induction of maximum DNA damage at highest concentration (1/3rd of LC50) of pesticide mixture. Statistically significant effects for both concentrations and time of exposure in terms of DNA damage were observed in treated fish as compared to control group. The results supported the use of Comet assay for evaluating the toxicity of pollutants which may be used as part of environmental monitoring programs.
... 
Sumaira Zareen1, Syeda Sadaf Zahra2, Ayeza Mehmood3, Muhammad Asadullah4* and Aish Muhammad5
...AP) or kinetin for shoot induction and Indole acetic acid (IAA) for primary shoot and Indole Butyric acid (IBA) for root proliferation. Best shoot proliferation (4.48 per explant) was observed in MS medium containing 0.2/0.4 mg/L BAP/IAA combination. For rooting of the micro shoots, MS medium was supplemented with 1.0 mg/l IBA exhibited best results with 4.69 mean value of roots per shoot at an average root length of 3.86 cm. After acclimatization and transpla...
Sadia Tehrim*, M. Yasin Mirza** and G. Mustafa Sajid*
COMPARATIVE STUDY OF DIFFERENT GROWTH REGULATORS FOR EFFICIENT PLANT REGENERATION IN GRAPES
...t proliferation and root induction) when combined with BAP. The media containing NAA, BAP and kinetin accelerated the growth in terms of shoot mass in two accessions, 4494(1) Kowar Sufaid and 4472(12) Harker Agoon and again the culture response was genotype dependent. There was a difference in the growth responses of the three accessions studied. The best rooting response was -1 obtained on 75% MS media containing 0.7 mgl , NAA. ...
Muhammad Shahbaz Aslam1, Iram Gull1, Zaigham Abbas2,* and Muhammad Amin Athar1
...n soluble form with IPTG induction to final concentration 0.5 mM at 37oC for 4 h and purified using affinity chromatography. SUMO tag was removed from SUMO-IFNα2-Tα1 by SUMO protease and recombinant IFNα2-Tα1 was collected in flow through by affinity chromatography. The MW (~23 kDa) of IFNα2-Tα1 was determined by 12 % SDS-PAGE and its integrity was confirmed by Immuno blot analysis using anti-interferon α-2 ...
Tengfei Lu1, Wenhua Pei1, Shuang Zhang2, Yangnan Wu1, Fenghao Chen2, Xiao Han2 and Weijun Guan1,*
...ke cells after procedure induction. The results showed that the AMSCs obtained from sheep demonstrated the features of multipotent stem cells. Therefore, AMSCs could be a good option for transplantation for treatment and regenerative medical projects.
...
Mohammed Zaki Fathy*
...ne was produced by rapid induction, long duration, smooth recovery and satisfactory anesthesia without any significant cardio-respiratory changes and produced good and produce efficiently anesthesia in xylazine pre-medicated Baladi Egyptian donkeys in compare with the propofol. ...
Rahim Dad Brohi1,*, Amir Bukhsh Kalhoro1, Allah Bux Kachiwal1, Illahi Bux Kalhoro1, Dildar Hussain Kalhoro1, Sarfaraz Ahmed2, Farhan Anwar Khan3, Hira Sajjad Talpur1, Zia-ur-Rehman1 and Dinesh Bhattaria4
...imens. The means of safe induction of anesthesia (propofol, 14.00 ± 0.2582; and thiopentone sodium, 14.83 ± 0.1667 seconds) and duration of anesthesia (thiopentone sodium, 14.50 ± 0.2236 and propofol, 13.22 ±0.1014 min), and a full recovery from anaesthesia (thiopentone sodium, 13.67 ± 0.4216; and propofol, 12.45 ± 0.2432 min) were differed from one another. Comparing the results of this study, it is concluded that the...
Soumble Zulfiqar1, Khuram Shehzad1, Sana Tahir1, Khalid A. Al-Ghanim2 and Abdul Rauf Shakoori1,2,3,*
...8.3 times - under copper induction. Time course study revealed a bimodal pattern of expression with two maxima, first at 15 min and second at 90 min exposure time. The role of CueO in copper detoxification as well as its laccase activity makes it suitable for biotechnological applications.
...
Asad Ullah1, Umar Sadique Khattak2, Sultan Ayaz1, Muhammad Subhan Qureshi2, Imad Khan1, Ibad Ullah Jan2, Irfan Khattak1, Raheela Taj3, Sahar Nigar4, Naimat Ullah Khan1,Mumtaz Ali Khan5 and Muhammad Luqman Sohail6,*
...T positivity. Similarly, induction of new animals into the herd (OR= 2.661; CI = 1.758-4.028) and sheltering the animals at night (OR= 2.448; CI = 1.568-3.882) also showed association with a positive skin test. The herd owners and animal handlers had poor knowledge regarding signs, symptoms and the zoonotic nature of the infection. Three primary signs (persistent cough, weight loss and fever) were recognized by 4.7% of respondents including herd owners, herder...
Song Jiang1,2, Fa-lin Zhou1, Qi-bin Yang1, Jian-hua Huang1, Li-shi Yang1 and Shi-gui Jiang1*
...an important role in the induction of many signaling pathways in response to thermal stress. Additional ATP was also produced after cold and heat stress, suggesting that more energy was required to cope with temperature extremes. Our findings indicate that temperature stress led to oxygen metabolism disorder, possibly due to the temperature being beyond the oxygen- and capacity-limitation of thermal tolerance of P. monodon. Oxygen metabolism disruption ...
Muhammad Akram and Faheem Aftab
THIDIAZURON INDUCES IN VITRO BUD BREAK AND SHOOT DEVELOPMENT FROM NODAL EXPLANTS OF ORTHOTROPIC SHOOTS OF MAIDENHAIR TREE (GINKGO BILOBA L.)
...tudy, axillary shoot bud induction, the growth of shoot and callus formation was observed. Shoots were cut to prepare nodal explants (5-10 mm long), surface sterilized and inoculated on MS (Murashig and Skoog, 1962) medium supplemented with different concentration (0.001, 0.005, 0.01, 0.05, 0.1, 0.5, 1,2,3,4 or 5uM) of TDZ for 24 days. Highest bud break (100%) was obtained at 0.005 uM TDZ after 14 days of initial culture. The same cultures were further maintai...
Muhammad Babar Khawar, Nadeem Sheikh*
Effect of paper industry leachate on various serological indices and serum proteins of wistar rats
...s revealed that leachate induction leads to significant positive variations in level of serum aspartate aminotransferases (AST) (P<0.0001), cholesterol (P<0.0001) and High density lipoproteins (HDL) (P<0.0001) while a significant negative change in triglycerides (P=0.0002) and creatinine (P=0.0370) level in both experimental groups. Alanine aminotransferases (ALT) level showed a significant increment in Group 1 and a decrement in Group 2 compared to c...
Tasleem Akhtar, Nadeem Sheikh*
Induction of acute phase in response to tacrolimus induced hepatotoxicity
Muddasir Hassan Abbasi1, 2, Noor Fatima1, Syed Shahid Imran Bukhari1, Asma Rashid khan3, Nadeem Sheikh2*
Variations in proteins and transaminases following experimental induction of Bisphenol A in mice
Rizwan Ullah Shah* and Iqbal Munir
...nd hypocotyl) for callus induction followed by shoot and root regeneration of Brassica carinata. Seeds were sterilized and germinated, of which one week cotyledon and hypocotyl were used for callus formation and shoot regeneration at five different level of 1-naphthaleneacetic acid NAA (0.05, 0.1, 0.5, 1.0 and 1.5 mg/l) and 6-benzyl amino purine BAP (0.1, 0.5, 0.7, 1.0 and 1.5 mg/l), and root regeneration at four levels of indole butyric acid IBA (0.05, 0.1, 0...
Ummad-ud-Din Umar1, Syed Burhan-ud-Din2, Muhammad Fahad Khan1, Ateeq ur Rehman1, Syed Atif Hasan Naqvi1*, Muhammad Asif Zulfiqar3, Azhar Ali Khan3 and Naila Ilyas1
... protection level by the induction of systemic acquired resistance. ...
Muhammad Aslam Rajput1,3, Imtiaz Ahmed Khan2, Rehana Naz Syed3 and Abdul Mubeen Lodhi3*
Aisha Khalid1, Muhammad Tayyab1,*, Abu Saeed Hashmi1, Tahir Yaqub2, Ali Raza Awan1, Muhammad Wasim1, Shagufta Saeed1, Sehrish Firyal1 and Abdul Rauf Shakoori3
...opyranoside (IPTG), post induction time, effect of temperature and pH were examined for the maximal production of recombinant cellulase. The effect of supplementation of LB medium with additional carbon and nitrogen sources was also analyzed for maximal production of recombinant protein. Higher level enzyme activity was recorded at 25°C, pH 7.0 when the cells were induced with 0.5 mM IPTG with 22h post induction i...
Hala Rajab1, Muhammad Sayyar Khan1*, Safdar Hussain Shah1 and Syed Mehar Ali Shah2
... calli, shoots and roots induction in a variety of genotypes. While false positive lines were obtained in the result of floral-dip method, the tissue culture based method led to successful regeneration of shoots on Murashige and Skoog (MS) media containing BAP 3 mgL-1 and developed roots in ½ MS media supplemented with IBA 3 mgL-1 in case of OSCAR cultivar. Successful selection of transgenic lines was achieved using selective antibiotic kanamycin 50 mg...
Betül Ayça Dönmez, Sarbesh Das Dangol and Allah Bakhsh*
...xplants. About 33% calli induction was achieved from transformation mediated by AGL1 strain in cv. Desiree, and about 23 % from LBA4404 strain in S. chacoense M6. This study will further aid in the development of transgenic stable transgenic potatoes, especially in diploid cultivars. ...
Awais Ahmed Khan1*, Zafar Iqbal1 and Muhammad Atiq2
...by H2O2 elevation and by induction of antioxidant enzymes. This study was planned to evaluate the organic elicitors and to determine the biochemical changes in citrus fruit. ...
Amna Qazi1, Ghulam Shah Nizamani2*, Muhammad Tahir Khan2, Shafquat Yasmeen2, Shahla Karim Baloch1, Muharram Ali1, Imtiaz Ahmed Khan2, Sagheer Ahmad3, Muhammad Rashid Nizamani4 and Mohammad Aquil Siddiqui2
...nt combinations of shoot induction media and three disparate recipes of rooting media. It was seen that both of the factors i.e. genotype as well as growth medium played significant role in inducing propagation of the sugarcane. Shoots were observed to initiate earliest in NIA-2004, NIA-2012 and NIA-1026-P7 in MS media supplemented with 1.00 mg l-1 IAA + 1.00 mg l-1 Kin + 1.00 mg l-1 BAP and 20 g l-1 sucrose. Regarding number of shoots, shoot length and number...
Hui-Ying Chen1,2*, Ping-Chuan Yin1,2, Ya-Nan Lu1,2, Hai-Yun Li1,2*and Yang Shan3
...artially mediated by the induction of apoptosis in cells. However, the underlying molecular mechanisms of aspirin in the prevention of cancer are yet to be fully elucidated. In the current study, the GSE115660 microarray dataset was downloaded from the Gene Expression Omnibus database to identify key genes in aspirin-damaged yeast cells following redox injury. Differentially expressed genes (DEGs) were subsequently identified and functionally enriched for anal...
Yuhong Li1, Dongxue Wu1, Xue Wang1, Mi Zhang1, Hanyu Zhu1, Zhe Shi1 and Fumin Feng1,2*
Muhammad Usman Ghazanfar1, Waqas Raza1*, Waqas Wakil2,3, Imtiaz Hussain4 and Misbah Iqbal Qamar1
...f the study was to study induction of defense responses and protective effects against Phytophthora infestans (Mont.) de Bary and sucking insect pests of potato (Solanum tuberosum L.) with application of salicylic acid (SA) and β-aminobutyric acid (BABA). Concentration of SA and BABA (2, 4 and 5 mM) were applied to foliage. Application of both resistance inducers suppressed disease development by 54.1% and 67.4%, for SA and BABA, respectively, 5 days afte...
Ming-Yan Shi2, Xu-Liang Yan1, Ping-Ping Han1, Yu-Jie Tang1 and Hui-Xia Li1*
...ved in SMSC adipogenesis induction, and the role of these miRNAs in Wnt/β-catenin signaling, has received little attention, particularly in livestock. In the present study, bovine skeletal muscle satellite cells (bSMSCs) were cultured and the Wnt/β-catenin signaling pathway was activated by LiCl. Investigation of the patterns of differentially-expressed miRNAs and analysis of bSMSCs target genes were then carried out using microarray and bioinformati...
Muhammad Mazhar Iqbal1*, Ijaz Ahmad2, Shahzad Maqsood Ahmed Basra3, Abu Baker Ijaz4, Zahid Hassan Tarar5, Muhammad Ansar6, Umer Iqbal7, Tayyaba Naz4, Bilquees Fatima8, Muhammad Akram9, Allah Wasaya10, Asif Iqbal11, Shakeel Ahmed Anwar12, Khalid Saif Ullah Khan13, Azeem Khalid14, Asif Aziz15 and Rashid Mehmood16
Huma Abbas1*, Nazir Javed1,Muhammad Kamran2, Sajid Aleem Khan1, Hira Abbas3, Ehetisham-ul-Haq2, Abdul Jabbar1, Mehwish Naz1 and Ihsan Ullah4
...als was evaluated on the induction of systemic activity by using modified split root technique. Efficacy of different application methods; soil drench and root dip was also tested. Results revealed that all the chemicals have more or less systemic effect against M. incognita. Cartap was found to be more effective in reduction of egg masses followed by Virtako in both treated and untreated root portion. Number of egg masses was reduced significantly in C...
Yihong Tian, Yongmei Qi*, Sajid Naeem, Ke Gao and Yingmei Zhang*
...ttributable to autophagy induction.
...
Tafail Akbar Mughal1, Shaukat Ali1*, Hafiz Muhammad Tahir1, Shumaila Mumtaz1, Samaira Mumtaz1, Ali Hassan1 and Syed Akif Raza Kazmi2
...betic phenotype. Alloxan induction raised the overall level of triglycerides, low density- lipids (LDLs), and cholesterol and reduced the level of high density lipids (HDLs). Oral administration of 200 mg/kg aqueous extract of B. lycium Royleroot bark for 28 days rescued all these changes. This study shows that the root bark extract of B. lycium Roylehas antihyperlipidemic properties.
Agustine Christela Melviana1, Rizkita Rachmi Esyanti1*, Roy Hendroko Setyobudi2, Maizirwan Mel3, Praptiningsih Gamawati Adinurani4 and Juris Burlakovs5
Gene Expression Related to Steviol Glycoside Synthesis Produced in Stevia rebaudiana (Bert.) Shoot Culture Induced with High Far-Red LED Light in TIS RITA® Bioreactor System
... by using a high-red LED induction to delay the flowering stage, which may contribute to higher biomass and glycoside concentrations through greater system productivity. Hitherto, there was no significant study about the effect of far-red LED induction to improve the steviol glycoside content of Stevia plants, particularly at the molecular level. This research was therefore performed in the TIS RITA® bioreactor sys...
Arifa Khan1*, Arsalan Bashir1, Shazia Erum2, Jabar Zaman Khan Khatak1 and Aish Muhammad3
Effects of 6-Benzylaminopurine and Indole-3-acetic Acid on Growth and Root Development of Banana Explants in Micropropagation
...mily) on growth and root induction of banana explants micro-propagated in vitro. Explants were obtained from young suckers of 8818-william, Pisang and Brazilian varieties. The explants were cultured in vitro containing MS media and different BAP and IAA combinations. The results showed banana varieties exhibited differences for the shoot and root development and also for the number of shoots and leaves. Wiallium-8818 hybrid gave more shoots (7.33) when the con...
Ying Liu1, Shankun Liu1, Hui Wang2 and Weihua Su2,*
...jection was used for the induction of diabetes. The rats were received the various dose of caffeic acid. After the pregnancy (19 day) the blood sample was collected via puncturing the retro arbital and collecting the fetus from all the groups. The rats, fetus weight and placental weight were calculated. The blood glucose level, serum, lipids, antioxidant, cytokines and C-peptide was recorded. GDM group rats showed the lower weight of pregnant rats, fetal and p...
Simeen Mansoor, Jabeen Farheen* and Meher Hassan
...ess. Thus, the impact of induction of thermotolerance by sublethal-temperature (40 °C), 100 µM indoleacetic acid (IAA), and 100 µM gibberellic acid (GAᴣ) before lethal-temperature stress (50 °C) were assessed on green gram growth and protein profile. For this purpose, the distilled-water and ...
Muhammad Usman Ghazanfar1, Waqas Wakil2, Shahbaz Talib Sahi3, Waqas Raza1* and Mahmood Ahmad Randhawa4
... plant amino acids after induction of resistance. Here we investigated the amino acid contents in induced and un-inoculated and invaded region after induction of resistance. The quantities of methionine, iso-leucine, leucine, tyrosine and phenylalanine contents in three (Pb-91, C-44 and Bittle-98) chickpea cultivars increased after induction and stress of A. rabiei and comparatively higher...
Hesham Saeed1*, Manal Abdel-Fattah1, Ahmad Eldoksh1, Farid S. Ataya2 and Manal Shalaby3
...weight of 22.5 kDa after induction of expression with IPTG for 5 h. The recombinant IFNα was expressed in the form of inclusion bodies that were separated and solubilized in vitro and the protein was refolded using SDS and KCl. The folded protein is then purified using on Ni-NTA Agarose affinity chromatography and the purity was judged by SDS-PAGE. Moreover, the effect of the recombinant IFNα of the viability of cancer cell line was assessed...
Md. Alal Hossen1, Mohammad Amzad Hossain1*, A.K.M. Munzurul Hasan1,2, Bipresh Das1, Sohel Mian1, Mohammed Mahbub Iqbal1
...thetic GnRHa was used as induction agent where male injected half of the doses of female and a control without S-GnRHa was assigned. The stages of embryonic development were observed under a photomicroscope. Average fertilization and hatching rates varied from 74.33% to 83.89% and from 72.33% to 85.11% respectively among different treatments and control females did not ovulate. The eggs of M. gulio was firmly adhesive and immediately after spawning the average...
Saba Aleem1*, Iram Sharif2, Mehvish Tahir3, Muhammad Najeebullah3, Ali Nawaz1, Muhammad Imran Khan1, Amina Batool1 and Waheed Arshad1
...by their ability to curd induction and development at high temperature and also based on different physiological traits. Heat susceptible genotypes showed lengthened curd induction stage. Further, decrease in chlorophyll and osmoprotectants contents was also seen in heat susceptible genotypes. TSX-C40 was identified as most susceptible genotype to heat stress due to low accumulation of glycine betaine and proline, and greate...
Fahrauk Faramayuda1,2*, Totik Sri Mariani3, Elfahmi1,4 and Sukrasno1
...t tissue culture (callus induction). This research aim is to identify secondary metabolite in callus using thin layer chromatography (TLC). The profiling of ethanol extracts of callus of two varieties O. aristatus were carried out using solvent system toluene-ethyl acetate- formic acid-water (3:3:1:0.2). The results of monitoring TLC suggest that callus obtained from Schenk and Hildebrandt (SH) media can proceed to the suspension culture stage because it...
M. A. Kararah1, Om-Hashem M. El-Banna1, Salwa N. Zein2 and Abd-Elrehiem, A.F.3
...div>
were used for induction of systemic acquired resistance, whereas camphor
oil was used as an antiviral agent to reduce PMV infection. Parahydroxy
benzoic acid was effective at 0.001% concentration, exhibited 88.7%
reduction when had applied 2h before inoculation. Also, salicylic acid was
effective as it reduced infection to 77.4% at 0.01% concentration when
applied 3h before inoculation. Whe...
A. A. Rezk1,3, K. A. Alhudaib2 and A. M. Soliman2,3
...e expression vector upon induction; the viral protein was expressed as 6XHis-tagged CYSDV fusion protein in Escherichia coli cells BL21 strain. The purified protein was immunized by injecting to New Zealand white rabbit using 6 injections at weekly intervals intravenously and subcutaneous. The rabbit blood was collected by bleeding and the antiserum was obtained. IgG was purified and conjugated to alkaline phosphatase. The conjugated antiserum was evaluated by...
Aly M. Abdel-Salam
...mmunization was used for induction of antisera for CYSDV and CVYV. RT-PCR was used to amplify coat protein genes for the two viruses using specific primers. DAS-ELISA and immuno-blotting were used for evaluating the induced antisera. Results: Antisera for CYSDV and CVYV were produced efficiently and used for virus diagnosis through DAS-ELISA, DBIA, and TBIA. RT-PCR confirmed the nature of the two viruses. However mixed infection was noticed for CVYV and CVYV u...
Huda S. Darwish 1, Om-hashem M. El-Banna2, Mohamed S. Abbas3, Hoda M. Waziri 1, Maisa A. Awad1
...ars and characterization induction mutagens by ISSR molecular method.
Methods: ToRSV was isolated from infected tomato, grapevine and pelargonium plants and detected
using double antibody sandwich-enzyme linked immunosorbent assay (DAS-ELISA). Host range study
was carried out using mechanical inoculation into fourteen different diagnostic host plant species and
cultivars belonging to 6 families. Tomato seeds were...
Atta Muhammad Memon1, Ubedullah Kaka2*, Muhammad Umer3, Asghar Ali Kamboh4, Atique Ahmed Behan5, Habibullah Janyaro6, Yong Meng Goh7, Amir Bukhsh Kalhoro6, Jalila Abu8, Khaleeq ur Rehman Bhutto9 and Arsalan Maqbool10
...ooth and excitement free induction of anaesthesia in pigeons. Ketamine produced surgical anaesthesia within 4.58 ± 0.68 min. Duration of anaesthesia was 55.79 ± 4.51 min. Vital parameters – pulse rate, respiratory rate and cloacal temperatures decreased significantly after premedication as well as during anaesthesia in the MK group which served as the control. The MKA group significantly increased (P < 0.001) the pulse rate, respiratory ...
Sajid Abdullah1*, Huma Naz2*, Zunera Abbas1, Uzma Nazir1, Mehrunisa Basharat1, Tanveer Ahmed3, Adnan Ahmad Qazi2
... attu showed significant induction of MN and DN in blood due to metals (Pb, Cr and Cd) exposure. It was noted that Pb and Cd exposure caused significant formation of MN and DN in RBCs of fish throughout the experimental period. However, Cr treated fish showed higher formation of MN and DN during initial two weeks of exposure after that both were decreased. The toxic potential of different concentration of Pb, Cr and Cd induced genotoxicity followed order: 1/3r...
Mazhar Ullah and Mohammad Sayyar Khan*
...ty-CP 77/400. For callus induction 2,4-D as an auxin was found to be most effective as compared to NAA. BAP and NAA in combination resulted in high regeneration capacities. Amongst the carbon sources, sucrose was most effective both for callus induction and regeneration. ANOVA revealed significant differences (P ≤ 0.05) amongst the callusing media (CM). Maximum callus induction (48.55%)...
Najeeb Ullah1*, Muhammad Nadeem2, Malik Mujaddad-ur-Rehman1, Rubina Nelofer2 and Yasir Arfat3
...re 35 °C, pH 9.0 and induction period of 96 hour. Almost 2.9 fold increase in protease production was observed after optimization at these optimal conditions. All these results indicated that skin waste from leather industry can be exploited as a potential substrate for alkaline protease production at optimized conditions by Bacillus sp.
Novelty Statement | In present research work industria...
Ayesha Muzamil, Hafiz Muhammad Tahir, Shaukat Ali, Iram Liaqat, Aamir Ali, Muhammad Summer
...an important role in the induction and suppression of inflammation. Cytokines are diverse form of proteins that act pro- and anti-inflammatory cytokines. The main component of immunity is IL-1. IL-6 cytokine play its role in regulation of metabolic reactions. The activity of various leukocytes is suppressed by IL-4 and IL-10 (anti-inflammatory cytokines) that triggers the production of pro-inflammatory cytokines. This review paper provides an overview of infla...
Muhammad Taqi1, Shazia Erum2, Shamaila Rasheed2, Sadar Uddin Siddiqui2 and Shakeel Ahmad Jatoi2*
...ed with NAA and IBA root induction. The highest rooting percentage (61.67%), as well as root length (3.4 cm) was obtained with 0.5mg/L of both IBA and NAA. The current study could be beneficial for providing the basis to restore wild populations of C. tuberculata using in-vitro raised mass propagated plants, and help conserve the endangered germs of hills....
Johar Hussian1, Faiz Muhammad2, Shafqat Fatimah Rehmani3, Aqeel Ahmad4, Nazir Ahmad Lone4, Moomal Bughio5, Syed Khurram Freed1 and Shakeel Ahmed Khan4*
...ot affected by enzymatic inductions. Therefore, the antigen prepared by phospholipase C has been found to be more effective for HI test for rapid diagnosis of IBV during infection....
Lin Huang1, Ling Mai2, Keyan Zhong1 and Xinjun Chen3*
...pET-30a(+)-SeFABP. After induction, the recombinant SeFABP was successfully expressed in the form of inclusion body. SDS-PAGE and Western blot showed a 15kD band, indicating that the recombinant was successfully purified. In conclusion, the characteristics of SeFABP and its high-purity recombinant protein were obtained, provides a basis for future research based on SeFABP in vaccine development and drug design....
Nafisa Shoaib* and Aisha Majid Ali
...ve been evaluated by the induction of micronuclei (MN). The aim of the present study was to assess the MN frequency in the haemolymph of green mussel (Perna viridis) after exposure to different concentrations of organophosphate pesticides (chlorpyrifos, malathion), synthetic pyrethroid pesticide (cypermethrin, lambda-cyhalothrin) and herbicide (buctril). Haemocytes of bivalve play an important role in immune defense and detoxification of contaminants. The biva...
Semra Okur Gumusova1, Gul Fatma Yarim2, Cuneyt Tamer1,*, Ayris Salt2 and Gokhan Inat3
Arman Sayuti1, Hamdan Hamdan2, Miftahul Jannah3, Safriadi Safriadi3, Teuku Nasri3, Tongku Nizwan Siregar2*
Mohamed Mohamady Ghanem1*, Yassine Mahmoud Abdelraoof1, Abdelghany Hefnawy Abdelghany2, Eman Abdelhamid El-Ebissy3, Ahmed Ragab Askar4,5, Attia Ahmed Eissa6
...vement after lactic acid induction. Hematological examination revealed a significant increase (P < 0.05) in RBCs, HB, PCV%, platelets and WBCs after induction of lactic acidosis. Biochemically, the induced lactic acidosis produced significant increase (P < 0.05) in ALT, AST, GGT, urea, creatinine with significant decrease (P < 0.05) in ALP, albumin, globulin, total protein, A/G ratio, Na, Cl, Ca, P and Mg. Examinati...
Ahmed Alazzouni1, Ashraf Al-Brakati2,*, Sherif Rabie1, Mohamed Gabry1 and Basmaa Hassan1
... for one month after the induction of leukemia. We found that the extracts ameliorated most histopathological changes in MLNs and spleens in rats exposed to benzene, restoring normal mast cell counts and elastic fiber area percent in a benzene-induced leukaemia model. To conclude Moringa oleifera, curcumin, and green tea extracts could be used as natural chemotherapeutic agents and as adjuvants to ameliorate histopathological changes associated with leukemia.<...
Zhongqing Wang1, Qiuyue Wu1, Ruiling Ye1, Fazul Nabi1,4, Yangfei Shang1, Sarfaraz Ali4 and Juan Liu1,2,3*
... except in CG. After the induction of the diarrhea, the pathological signs and the quantity of iIELs in small intestine and the mRNA level of IL-6 and TNF-α in duodenum were analyzed by qRT-PCR from all groups at 6 h and 4 d, respectively. IG group shows abnormal mental state and the lack of the appetite; however, AMP pre-treatment caused the normal state and appetite. The duodenal mucosa in IG was seriously injured; with epithelial hyperplasia and necro...
Amjed Ali1, Muhammad Tayyab1*, Abu Saeed Hashmi2, Asif Nadeem3, Shumaila Hanif4, Sehrish Firyal1, Shagufta Saeed1, Ali Raza Awan1 and Muhammad Wasim1
... 0.6 mM IPTG with a post induction time of 5h at 37 °C. The characterization studies demonstrated the maximal enzyme activity at 37 °C in 50 mM sodium phosphate buffer pH 7. The presence of EDTA in the activity assay mixture reduced the β-lactamase activity to 91% while Zn2+, Co2+, Mn2+ enhanced the enzymatic activity to 144, 121 and 108% when used at a final concentration of 1 mM. The ionic and non-ionic detergents showed slight inhibitory impact...
Muhammad Kashif Iqbal1*, Khalid Mehmood2,4, Shakeel Ahmed3, Fazul Nabi4, Muhammad Arif Rizwan1, Muhammad Kaleem1, Mushtaq Ahmed1, Abdul Waheed1 and Jiakui Li5
...oup. Furthermore, thiram induction also increased the levels of AST, ALT and MDA contents in liver, whereas decreased the antioxidant enzymes (SOD; GSH-Px) and ALP values in thiram group, while these values were found close to normal range in FK228 group as compared to control group in response to FK228 treatment. In conclusion, VEGF and Flk-1 genes play an important role in the formation of avascular growth plate, whereas FK228 can heal lameness and avascular...
Chemoprotective Effect of Daphnetin against Benzene Induced Leukemia Cancer via Alteration of CYP2E1
Junling Liu, Chen Sun, Qiao Yu, Yanzhi Liang, Shanshan Lin and Meng Tian*
...Benzene was used for the induction of leukemia in experimental rats. The rats were divided into different groups and received the daphnetin (12.5, 25 and 50 mg/kg). The body weight, haematological parameters, deoxyribonucleic acid (DNA) fragmentation and cell cycle regulatory parameter were also estimated. Reverse transcription polymerase chain reaction (RT-PCR) was used for the estimation of messenger RNA (mRNA) expression of cytochrome P450 2E1 (CYP2E1). Dap...
Muhammad Shafiq1, Tehseen Ashraf2*, Sehrish Mushtaq1, Naveeda Anjum3, Muhammad Asim4, Muhammad Aqeel Feroze3, Malik Abdul Rehman4 and Marja Aziz3
...ted. Maximum mean callus induction rate of hypocotyl was 76.6% in SP followed by TP (73.3%); Sanam (57.3%); Loungi (43.3%) respectively. Whereas in case of cotyledon maximum inductions rate was found in Loungi (62.0%) followed by SP (60); Sanam (57.3); TP (46) individually. According to the observations maximum plant regeneration was found in genotype SP (9%), followed by Loungi (1.8%); Sanam (1.5%) but no plant regeneration...
Ahmad Nadeem1,2 and Rubina Arshad1,2,*
...irradiation for mutation induction. Seeds of two chickpea varieties were inoculated with LAB isolates in four treatments comprising of T1 (wild type LPA6), T2 (mutant MLPA6), T3 (wild type LPA6 + mutant MLPA6 consortium) and T4 (un-inoculated control). Inoculated and uninoculated seeds were sown in net house in three replicates. In kabuli variety, maximum increase in plant height (16.7%), root length (5.9%), number of secondary branches (19%), pod number (63.2...
Florence N. Nworah*, Nene O. Uchendu, Ifeoma F. Chukwuma*, Emmanuel C. Ossai and Emmanuel C. Ezeako
...Iron and Zinc. After the induction of myelosuppression, there was a significant (p<0.05) decrease in haematological parameters and a significant (p<0.05) increase in the concentrations and activities of oxidative stress biomarkers. However, treatment with the blood of P. sinuatus reversed the effect of cyclophosphamide on the haematological parameters and the oxidative stress biomarkers. These results suggest that the P. sinuatus blood exhibits both haem...
Mohamed M. Bahr1, Mohamed S. Amer1*, Khaled Abo-EL-Sooud2, Shaimaa M. Kamel3, Marwa A. Fouly4, Ahmed N. Abdallah5, Ashraf A. Shamaa1, Omar S. El-Tookhy1
...s (6 rabbits per group), induction of corneal ulcers centrally in groups (I, II) and peripherally in (III, IV). Treated groups are (I, III) using topical lyophilized canine Mesenchymal Stem Cells derived EVs & control groups are (II, IV) using topical normal saline. Result: both treated groups appeared fluorescein negative at the 5th day and are characterized by rapid reepithelization significantly earlier than control groups which were still fluorescein p...
M.M.A. Youssef and A.M.S. Lashein†
Saima Mumtaz1,3*, M. Imran Kasana1, Riaz Alam1, Noorullah Khan1, Muhammad Noman1, Sanjeela Sabahat1 and Hussain Shah2
...ina and Bedana) for root induction, its development and success rate of survival in hardwood cuttings. Six treatments of IBA (1000, 2000, 3000, 4000, 5000, 6000ppm) and one with control (without IBA) were applied at base of hardwood cuttings (6mmØ) of different litchi cultivars. Statistically analyzed data showed that root induction, root growth and survival of rooted cuttings was significantly influenced by IBA conce...
Muhammad Adeel Qureshi1*, Raza Ahmad2, Bilal Ahmed1, Tanzim Ullah Khan1 and Muhammad Yasin3
...best protocol for callus induction and plant regeneration of potato (Solanum tuberosum L.). The experiment was carried out in complete randomized design (CRD). Leaves and internodes of potato cv. Desiree were used as explant for callus induction and plant regeneration. Two types of callus induction media (CIM) were used, i.e. CIM1 and CIM2 with concentrations of 2, 4-D 2mg/lit and 3 mg/lit...
Shah Murad Khan1, Hamayun Khan1, Younas Muhammad1, Haq Aman Ullah1*, Muhammad Tariq Tunio2, Ali Gohar2 and Fazli Rabbani3
Chenchen Liu1,2, Tong Wang1, Muhammad Khan3*, Xiaolin Cui2 and Yongming Li1,2*
...echanism associated with induction of apoptosis includes ROS generation, G2/M phase arrest and inhibition of aerobic glycolysis. Inhibition of glycolysis was found to be linked with down-regulation of lactate dehydrogenase A (LDHA) and glucose transporter-1 (GLUT1). Finally, ALT effectively suppressed growth and induced apoptosis in cisplatin resistant A2780/CR cells. Taken together, our findings suggest that ALT could be developed into a lead for effective tr...
M. A. Abou El- Nasr1, Kh. A. Dougdoug1, Hayam S. Abdelkader2, and Rehab A.
Dawoud2
...100 plasmid vector. Upon induction, the viral protein coat gene was expressed as 6xHis-tagged PPV/CP fusion protein in E. coli M15 cells. The fusion protein was confirmed by western blot analysis....
Muhammad Akram¹,²*, Rashid Mahmood³, Fahim Arshad4 and Umer Farooq Gohar5
...As a result, 100% callus induction was achieved on the medium containing 1.0 µM TDZ. Notably, calli grown on 1.0 µM TDZ exhibited greater potential for shoot regeneration and proliferation in the growth regulator-free MS liquid medium (95.25%) as compared to the solid medium (89.00%). Shoot cultures remained viable for up to 15 days without refreshing the medium and could be sustained for 12 months when sub-cultured every 15 days. Invigoration of a...
Shaimaa A.Tawfik2,3, EL S.T. Awad1*, Hoda O.Abu Bakr1, Amira M.Gamal-Eldeen4, Esmat Ashour2, Ismail M.Ahmed1
...esulted in an observable induction in CYP3A4 and MRP-1, while CSO/DOX showed an inhibition in both proteins. c-MYC was dramatically decreased in DOX group, while CSO/DOX group restored cellular c-MYC. CSO/DOX group resulted in a remarkable inhibition in the tumor suppressor miRNA (let-7a) compared to DOX-induced expression. Moreover, miR-122, as negative regulator of DOX resistance, showed a dramatic induction in CSO/DOX gr...
Aamir Ali, Hafiz Muhammad Tahir*, Azizullah, Shaukat Ali, Muhammad Summer, Ali Haidar Gormani, Saira Nawaz and Ayesha Muzamil
...injected to mice paw for induction of pain. These extracts significantly decreased edema in mice paw. The level of TLC and RBCs was increased to normal. Platelets level was also increased in mice treated with mulberry leaves extracts. Furthermore, these extracts significantly reduced the inflammatory pain and licking response triggered by injection of formalin in mice paw. These findings showed that the hot water extracts of leaves of these two plant species p...
Mohamed Mahrous Amer1*, Hanaa Sayed Fedawy2, Hoda Mohamed Mekky2, Khaled Mohamed Elbayoumi2, Ahmed Ali El-Shemy3, Mohamed Abd El-Rahman Bosila2
Muhammad Ilyas Riaz1, Masood Rabbani1*, Sohail Raza1, Ali Raza Awan2, Aleena Kokab1 and Iqra Nazir3
...t of residual virulence, induction of abortion, diagnostic interference, and human pathogenic potential. In this study, we have developed a Brucella abortus pUC19-K-UP-DN plasmid cassette for the construction of ΔpurD deleted mutant by deleting the purD gene which is responsible for de novo purine synthesis. For this, the pUC19-K-UP-DN plasmid cassette was initially constructed using pUC19, kanamycin, and purD upstream and downstream fragments. Then the ...
Bayanda Mdoda*, Faith Machabi, Busisani Wiseman Lembede, Eliton Chivandi
Abnet Mekonnen1*, Kadir Abderehman1 and Feti Seyaka2
... a very rapid and smooth induction and recovery. Pre-anesthetic medications are used to decrease fear and apprehension, aid in restraint, decrease the amount of other anesthetic agents required and decrease side effects of induction drugs. These drugs are metabolized by the liver and excreted by the kidneys. Monitoring vital signs continuously during anesthesia will provide early warning of potential problems and emergencies...
Rabail Hassan Toor1,3, Raazia Tasadduq2, Jane B. Lian3, Janet L. Stein3, Gary S. Stein3 and Abdul Rauf Shakoori1*
...st activity. Upon RANK-L induction of RAW264.7 cells to differentiate into osteoclasts, we found 10ng/ml CQ-H as most efficacious concentration for inhibiting osteoclastogenesis with 57.8% inhibition of TRAP activity. Whereas, in comparison 20ng/ml RSV showed 49.9% inhibition of TRAP activity. Expression of osteoclast marker genes such as NFATc1, ACP-5, CTSK, MMP-9 and CTR corroborated our results. Distinct downregulation of marker genes was observed in CQ-H a...
Ali Mosa Rashid Al-Yasari1, Zahid I. Mohammed2, Haider S. Almnehlawi3,4, Ali F Bargooth5, Nawar Jasim Alsalih1, Huda F. Hasan6*, Mohenned A Alsaadawi1
...roups as a result of the induction of diabetes. Comparing the STZ group to the control and camels’ milk groups, the blood glucagon level was the highest in the STZ group. Comparing the diabetes group to other groups, immunohistochemical analysis revealed a significant reduction in insulin secretion cells. In contrast, considerably more glucagon-producing cells were seen in the STZ group. Data from the camel milk group demonstrated its capacity to lessen ...
Irfan Safdar Durrani*, Noreen Asim and Ammar Sohail
...volved in plant hormonal inductions, developments and biotic and abiotic stress response. Notably, the OsGLP12-3 gene promoter from Dilrosh-97 was found to contain higher number of TATA-box and CAAT-box as compared to Nipponbare, reflecting it to be efficient in transcription initiation. Pairwise alignment using NCBI Nucleotide Blast tool showed 99% similarity between subject sequence (Nipponbare) and query sequence (Dilrosh-97). Localization of Transcription ...
Hanaa H.A. Gomaa1*, Dalia Y.A. Amin1, Mona A. Ismail1, Basma Hamdy2, Khaled A. El-Dougdoug3
...been suggested that, the induction of systemic acquired resistance was successfully achieved and protected fig plants against FLV infection.
...
Madbouly*, H.M.; Saif**, M.A. and Hussein* , A.S.
...ect of curcuma is in the induction of protection level 80% in treated but not NDV vaccinated group which equivalent to that group vaccinated with NDV vaccine only and not treated. From the obtained results we recommend the use of curcuma longa powder in poultry rations for enhancing the immune response against either field infection or vaccination....
Mahdyl , A.M.M.; Hafezl , M.A.; EL-Dougdoug2, Kh. A.; Fawzyl , R.N. and Shahwanl , Eman S.M.•
...cylic acid (SA) resulted induction of protein related to biotic inducers, virus concentration and disease severity (DS). The obtained results from quantification of total SA in induced tomato plants (after 7 days of spraying inducers) showed high level of SA with kombucha &eatment followed by C. inerme and M. jalapa, while mixed (M. jalapa+C. inerme) gave the lowest level of SA compared with healthy and infected controls. On the other hand, after virus ino...
El-Sayed • l , Eman H; Mahfouze , Sherin A.; Shaltout ,A. D.; El-Dougdoug3 ,Kh. A. and Sayed1 , R. A.
...om (1, 2 and 3 mg/l) for induction of mutation. In vitro screened plants after rooting were hardened and acclimatized in the glass house and were shifted into the field. Field screening was carried out against two different isolates of (BBTV and BMV) by using syringe method of inoculation. It was observed that, only two banana plants of the concentration 6 mg/l from 2, 4-D was found to be resistant against BMV isolate. In the same time, it is not BBTV resistan...
Fadia M. Attia and Hanaa H. A. Gomaa
... level of viremia before induction of therapy. In addition, complete blood count, prothrombin time and liver function tests were performed. The results showed that leucopenia and thrombocytopenia are more frequent in HCV patients with moderate degree of viremia than patients with mild viremia than those with no viremia (p<0.05). Moderate neutropenia (< 1000 /ul) and moderate lymphopenia (< 1000/ul) are observed in all patients with different levels of...
M.A. Abo-EInasr l, Kh.A. EL-Dougdougl, M.H. El-Kattan2 and L.A. Salem2
Nurlan Akhmetsadykov1*, Tanatar Kydyrov2, Moldir Akhmetzhanova1, Gulnazi Akhmetova3, Maxat Berdikulov4
...-1-thiogalactopyranoside induction at 2℃ for 18 hours was the most optimal condition for expression of recombinant GM6 T. evansi antigen. From a 500 ml of growth medium, a total of 3.4 mg of recombinant protein was successfully obtained. A recombinant GM6 antigen of T. evansi encoding a 30 kDa polypeptide was developed, which has diagnostically significant sensitivity in the enzyme immunoassay at a dilution of 1:6400 with sera of horses and donkeys infected ...
Sena’a M. Hussein1,2, Raffal A. Omar3*
...monstrates safety, rapid induction, and excellent analgesia, ensuring smooth recoveries suitable for surgery without complications. ...
Rondius Solfaine1, Faisal Fikri2, Salipudin Tasil Maslamama3, Muhammad Thohawi Elziyad Purnama4, Iwan Sahrial Hamid5*
...vealed that CCoV vaccine induction in the treatment group resulted in duodenal mucosa haemorrhage, necrosis, and severe inflammation. Conversely, in the group treated with Lactobacillus acidophilus, tissue damage was inhibited, demonstrating the protective effect of the probiotic. Lactobacillus acidophilus effectively mitigated lung, liver, and duodenal damage by reducing the expressions of TNF-α and IL-1β in mice.
Key...
Taghreed Jabbar Humadai*, Bushra Ibrahim AL-Kaisei
...tem and oxidative stress induction in male albino rats. The methods included sixty male albino rats at age 8-9 weeks and weighted 190-200 gm were divided into three groups: The first group: Consisted of 20 rats control were given distilled water and normal rat pellet, the second group: Consisted of 20 rats injected weekly 0.5µg /kg. b.w. by I/P route which represented 1/20 LD50 of tetrodotoxin, the third group: It also consisted of 20 rats injected weekl...
Muhammad Atif Raza1, Muhammad Tariq Javed2, Muhammad Fiaz1*, Muhammad Shakeel3, Muhammad Shahbaz Ul Haq2, Amna Kanwal2, Syeda Maryam Hussain1 and Muhammad Zubair Siddiqi4
...biotic is leading to the induction of antimicrobial resistance in microorganism. Main aim of this study was to find out substitute of antibiotic against Fowl Typhoid in broiler in terms of immunological, serum biochemistry and lipid profile parameters. Broiler chicks (Age = 1 d and n = 90) were kept under uniform management conditions. Chicks were divided randomly into six groups on day ten of their age with 15 replicates in each; control negative (CN), contro...
Qurrat ul Ain Shafique1, Sana Batool2, Hanfa Ashfaq2, Asima Tayyab2, Roquyya Gul3 and Mahjabeen Saleem1*
...TG and lactose) and post induction time. Maximum expression of IL-2 was achieved in LB with 0.5 mM IPTG for 6 hours post induction time. A denaturing purification scheme (Immobilized metal affinity chromatography) was employed for the purification of IL-2. The refolding of the purified IL-2 was achieved by stepwise dialysis method using urea gradient (8M-0M). Human IL-2 was recovered from 1 litre culture to a purity level of...
Abdelghafour El-Hamzaoui*, Mouloud Lamtai, Laila Ibouzine‑Dine, Sofia Azirar, Mohamed Yassine El-Brouzi, Ayoub Rezqaoui, Aboubaker El-Hessni, Abdelhalem Mesfioui
...of being involved in the induction of affective disorders. The present study was undertaken to examine the dose-dependent impact of GBH exposure over 8-week period on anxiety- and depression-like behaviors in male Wistar rats, administering daily subcutaneous injections of four different doses 25, 50, 75, and 100 mg/kg of GBH while a control group received 0.9% NaCl. Based on behavioral tests (the open field, elevated cross maze, and forced swim), behavioral a...
Quart ul Ain1, Arfan Ahmad1*, Aftab Ahmad Anjum1 and Mamoona Chaudhry2
...ucing virus shedding and induction of humoral immunity however local vaccines presented robust reduction compared to imported vaccine and can be used preferably due to cost effectiveness and availability to combat avian influenza H9 in poultry birds....
Muhammad Afzal and Aqeela Mobeen Akhter
...re. Successful bud shoot induction and shoot elongation was achieved on MS media with 5µM BAP concentration. High multiplication rate i.e.12 micro shoots per culture was obtained with 15µM BAP. After 4-5 weeks the shoots developed roots in the same media without adding any rooting hormones. These plantlets showed a good growth response in earthen pots and 85% survival of plants in soil was recorded....
Q. N. Javeed, Rehana Perveen, Imtiazul Haq and Ihsan Elahi
...drolysate was studied on induction and further growth of callus. Auxins were NAA (0.05, 0.1, 0.5 and 1.0 mg/1). Cytokinin used was BAP (1.0mg/1). Our results indicated that size of the explant, presence or absence of apical bud, controlled conditions of light and temperature are important factors in callus formation. A successful callus could only be raised on MS medium supplemented with 1.0mg/1 of 2, 4-D, CM10%, sucrose 2%in diffused light at 25 ± 20&de...
Sony Eka Nugraha1*, Marianne Marianne2, Rony Abdi Syahputra2, Aji Najihudin3, Nikmatul Ikhrom Eka Jayani4, Shaum Shiyan5, Nabila Nabila6,7
... (BW) for 10 days. After induction, rats were administered C. papaya leaves ethanol extract at various concentrations (50, 100, and 200 mg/kg BW) for 20 days. On day 21, the duke method was used to examine the bleeding time, and the blood clotting time was evaluated using the Lee White method. Blood samples were evaluated for platelet concentration, thrombopoietin concentration, prothrombin time, and active partial thromboplastin time. These results indicated ...
Amal Mohammed Ali Alah Al-Marmadhi*, Saadeya Ali lefelef Al-Gnami
...cles on female rats with induction of the hyperthyroidism. Fifty mature female rats (aged 90 days and weighted 150±10 g) have been randomly assigned to 5 equal groups (10 each),control (C) group , T1,T2,T3 and T4 groups were drenched with thyroxine (300mg/kg of B.W for 28 days ) for hyperthyroidism induction, T2 was drenched with methimazole (2.5 mg/kg of B.W for 30 days), T3 was drenched with allicin nanoparticles (5...
Syed Saqlain Hussain1*, Muhammad Rasheed2, Zammurad Iqbal Ahmed2, Ghulam Jilani3, Muhammad Irfan4, Muhammad Kashif Aziz5, Fiaz Hussain1, Muhammad Akhlaq Mudassar1 and Zuhair Hasnain2
Naveed Hussain1*, Muhammad Arif Khan1, Asim Khalid Mahmood2, Muhammad Yasin Tipu3, Mamoona Chaudhry4, Hamad Bin Rashid1, Haroon Akbar5 and Abdul Aziz6
...ng in osteogenesis, osteoinduction and osteoconduction. Orthobiologics are biological materials used to augment bone and soft tissue cure, with multifactorial effects. In our study, three orthobiologics i.e., Bone Marrow Aspirate (BMA), Platelet Rich Plasma (PRP) and Decellularized Fish Scale (DFS) were used in conjunction to autogenous bone graft. Objective was to transplant as many cells as possible with osteogenic potential along with the graft. These three...
Humam T. Hadi1*, Orooba M. S. Ibrahim2
...second part involved the induction of psoriasis in mice using 5% imiquimod cream, followed by topical application of different concentrations of SaAuNPs, clove oil, and salicylic acid. The second part evaluated the effectiveness of SaAuNPs in reducing psoriasis symptoms in mice. The findings showed that SaAuNPs had a significant (P≤0.05) anti-inflammatory effect, with the groups treated with 0.003% and 0.005% SaAuNPs showing a significant reduction in psori...
Maysaa A. Jumaah*, Ali A. Al-Iedani
...alum performed better in induction of adaptive immunity than the rest of adjuvents used. ...
Hafizuddin1, Sri Wahyuni2, Tongku Nizwan Siregar1*, Amalia Sutriana3, Ririn Triyuliani4, Siti Hanyyah Herviani4, Muhammad Farhan Syuhada4, Nur Salimah4, Muharni Mardiah Eriswandi4, Ratu Permata Ayu4, Putroe Hayatun Nufus4
...its after superovulation induction with BPE. The sample comprised of 1 local male rabbit and 9 local female rabbits with a history of giving birth and weighing between 1.8-2.2 kg. The animals were adapted for 30 days in separate cages and provided with feed and water ad libitum before treatment. The rabbits were divided into 3 treatment groups (n=3), the first group as a control group (R1) was injected with physiological NaCl, while group two (R2) and group th...
Bilal Ahmed Qazi, Nowsherwan Zarif, Anwar Ali*, Faizan Ahmed, Asim Karim and Ali Nawaz
...utcomes regarding runoff induction, survival rates, and the growth of tree species. This review recommends adopting specific water harvesting techniques for dry land forestry and range improvement in different geographical areas based on these research findings....
Essa D Alhtheal1*, Sufian S Salman2, Salah M AL-Kubaisi3
...ed red blood cells after induction of aplastic anemia in adult male dogs decreased from 2 on day 0 to 0.61 On day 15 for the positive control group, from 2.13 to 0.8 for the first group, and from 2.1 to 0.3 for the second group and from 2 to 1.07 for the third group and in the induction stage before giving it the treatment compared to the negative control group, respectively. In a hematological examination, the means of RBC ...
Eslam F.M. Eisa1, Bardees K. Elgohary1*, Mahasen El Shair1, Hagar F. Gouda2, Ali E. Kandeel1
...15 until 90 minutes post-induction, along with end-tidal sevoflurane, tidal volume, end-tidal carbon dioxide, oxygen saturation. The results revealed that G2 and G4 demonstrated superior hemodynamic stability, with more consistent in HR and MAP, reflected by the lowest coefficient of variation (18.7, 20.5% for HR; 15.8, 20.5% for MAP) throughout anesthesia and during the surgical procedure (2, 6% for HR; 5, 6% for MAP). In conclusion, combining a low dose of k...
Naglaa A. El-Taib1*, Asmaa T. Talayea2, Hanan R. Ghanayem3
...orine was more potent in induction E. coli O157 into VBNC state after 30 min. of application rather than hydrogen peroxide which failed in inducing the bacteria into VBNC at the same time points. The PMA-qPCR method, which was used to find the rfbE gene of E. coli O157, showed that the VBNC cells were still alive and working. This suggests that PMA-qPCR could be a useful way to find the VBNC state. This study concluded that in all treatments, whether by using ...
Noorulhuda A. Mahdi, Nadia H.R. AL-Falahi*
...row clot had a good osteoinduction properties in segmental bone defects regeneration.
Keywords | Segmental bone defect, Fish swim bladder, Bone marrow clot, Guided bone regeneration, Rabbits
...
Ashraf B. Said*, Ibrahim A. Ibrahim, Hoda I. Bahr, Marwa A. El-Beltagy
...5mg/kg b.w/i.p) for T2DM induction. The diabetic one was allocated to 6 groups (10 each) based on their treatment: untreated group, metformin (Metformin HCl, 500mg/kg b.w/day), GLP-1 (Trulicity,1.5mg/kg b.w/week), BM stem cells (IV injected with 1x106 units/once), GLP-1 plus metformin and the last group BM stem cells plus metformin for 4 weeks. After 4 weeks, rats were sacrified, blood samples are collected to measure FBG, FINS, C-peptide, and HOMA IR. TNF, IL...
Jaafar Haider Abd Alrudah1, Nada Fadhil Abbas2*, Farah Ali Hadi1, Haider Tuma Kaab3
...o demonstrate the immune induction potentials in pigeons. ...
Abbas A. Khudhair1*, Mohammed A. Qathee2, Muhsin S.G. Almozie’l3, Kassim F. Abdulkarem4
...ale rats were used after induction of hyperlipidemia through cholesterol administration, divided in to five groups and treated with different concentrations of pumpkin and rousovastatin. Pumpkin group (treated with 200mg/kg), rousovastatin group (treated with 20mg/kg) and comb group (treated with 20mg/kg rousovastatin and 200mg/kg pumpkin seed oil). Pumpkin and rousovastatin groups showed a significant decrease (p<0.05) in total cholesterol, triglyceride, L...
Qurat-ul-Ain1*, A. Ahmad2, Farhat Nazir Awan3, M. Rabbani1 and M. Hassan Mushtaq4
...MK084980) and tested for induction of humoral immune response in comparison with commercial vaccine in experimental cattle calves. Calves of group A (n=5) and B (n=5) were vaccinated with self-prepared and commercial vaccines respectively followed by boosting 30 days post primary vaccination, while group C was kept as non-vaccinated control group. Blood samples were taken from each group after every 2 months up to one year post vaccination and tested through A...
