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Frank Griffel

...equent readers the front page needed no further explanation. Foreign observers, however, did not understand so quickly and when a journalist of Slate.com asked him to explain, Luz said that the drawing is a callback to an earlier 2011 cover showing the Prophet Muhammad with the words: “100 lashes if you don’t die of laughter.” On the day that cover appeared, the offices of Charlie Hebdo were attacked by a firebomb. Luz explained Charlie Hebdo...

Yashab Tur

 

Science, Religion and Culture, Vol. 2, Iss. 2
...out the emergence and propagation of life on earth which, to the lay believer, seemed outright un-Godly. Marwa Elshakry, now an Associate Professor of History at Columbia University, explores the encounter of the Arab world with these and many other Western technologies and ideas in her wide-ranging Reading Darwin in Arabic through a panoramic lens, which then zooms in to focus on specific individuals. Thus we meet Free Masons, missionaries, colonial agents, o...

John Gachohi, Simon Karanja, Salome Wanyoike, Nduhiu Gitahi, Salome Bukachi, Kenneth Ngure

...abitats in Leptospira propagation. Influential habitats would serve as key targets for interventions. To achieve this aim, we have designed a pseudo-longitudinal survey to be undertaken for two years in Kibera slums in Nairobi city Kenya. Human and animal blood and urine samples will be collected at the household level whereas soil and water samples will be collected at the community level. Attempts will be made to isolate Leptospira bacteria from urine, soil ...

Abdur Rahim1, Ghulam Abbas1, Muhammad Naeem2, Sara Ferrando3, Lorenzo Gallus3, Noor Khan4, Muhammad Hafeez-ur-Rehman4, Abdul Ghaffar5 and Abdul Mateen6

...ck fin sea bream (Acanthopagrus berda), grunts (Pomadasys hasta), croaker (Otolithus ruber), silver sillago (Sillago sihama), sea perch (Lates calcarifer) and jaw fish (Johnius goma). In majority of processing units, traditional methods were applied causing quality problem. Finally, it was concluded that quality of fish meal and production in our region is suitable for fish feed formulation. But the processing technique should be improved to match internationa...

Abdur Rahim1, Ghulam Abbas1*, Lorenzo Gallus2, Sara Ferrando2, Muhammad Hafeez-ur-Rehman3, Abdul Ghaffar4 and Abdul Mateen5

...ck fin sea bream, Acanthopagrus berda (weight 23.5g−125.8 g) were investigated. Fish were randomly distributed in rectangular glass tanks (150 liter) and were fed at six ration levels of 2.0, 2.5, 3.0,3.5, 4.0 and 4.5% body weight per day (BW d−1) and four feeding frequency i.e., once, twice, thrice and four times per day. Fish in all replicates were fed with diet comprising 40% protein and 20% lipid for 75 days. Higher percent weight gain (% WG), ...
Abdur Rahim1, Ghulam Abbas1,*, Muhammad Naeem2, Sara Ferrando3, Lorenzo Gallus3, Muhammad Hafeez-ur-Rehman4, Abdul Ghaffar5 and Abdul Mateen6
... fin sea bream Acanthopagrus berda was investigated. Fish juvenile (10.1±0.5 g) were collected from Sonari Channel, Hawksbay, Karachi and were brought to aquaculture laboratory of the Centre for 15 days acclimatization. After acclimatization, they were randomly distributed into the rectangular tanks (3 × 1.5 ×1.5 ft each). In each tank, 10 fish were stocked with three replications for each treatment. Four isonitrogenous diets containi...
Abdur Rahim1, Ghulam Abbas1,*, Sara Ferrando2, Lorenzo Gallus2 and Abdul Ghaffar3
...fin sea bream, Acanthopagrus berda was examined in brackish water ponds. Fish were stocked @ 1500 individuals per pond (2 treated and 2 control) of approximate dimensions, 13.72 m × 10.97 m × 1.52 m (229 m3). In treated ponds, fish were fed with artificial diet (protein 42%, lipid 20% and energy 25.2 kJ g-1) for 120 days in three equal meals. On the other hand, control ponds remained without additives. During the whole ...

Muhammad Waqas1*, Anwar Ali Shad1, Omaira Bashir2 and Mohsin Iqbal3

...ts in sunflower oil and spaghetti. Tomato peel contained 720 mg/100g of carotenoids consisting phytoene (3.15%) followed by phytofluene (2.31%), β-carotene (2.11%), cis-lycopene (1.71%), lutein (1.51%), cis-ζ-carotene (0.61%), ζ-carotene (0.56%), and γ-carotene (0.52%). Lycopene was found in highest amount (87.52 %). Antioxidant activity of carotenoids from tomato peel was assessed by measuring Rancimat’s test and diphenyl-1-picrylhy...

Wesam Hasan Mohamed Mady 1*, Bing Liu2, Dong Huang2, A. Arafa1, M.K. Hassan1, M.M. Aly1, Pucheng Chen2, Yongping Jiang2* and Hualan Chen2

B. Ramanujam, R. D. Prasad, S. Sriram and R. Rangeswaran

Mass production, formulation, quality control and delivery of Trichoderma for plant disease management
...om the counts of live propagules in the formulation, bioefficacy also should be taken as a quality parameter to ensure availability of better products to farmers. Keywords : Trichoderma, mass production, formulation quality control, registration delivery, shelf-life, disease management.
 

 

...

Debjani Chowdhury, P. C. Paul and B. Dasgupta

Management of leaf spot of Centella asiatica (Thankuni) caused by Alternaria sp. and target leaf spot of Rauvolfia serpentina (Sarpagandha) caused by Corynespora cassicola
...Rauvolfia serpentina (Sarpagandha) caused by Corynespora cassicola by spraying three fungicides (carbendazim at 0.1%, mancozeb at 0.025% and blitox at 0.25%) and four biocontrol agents (Trichoderma viride at 107 cfu / ml, T. harzianum at 107 cfu / ml, Pseudomonas fluorescens at 108 cell / ml and Biomonas-a formulated product of P. fluorescens preparation at 0.15% for three times at an interval of 15 days. The results revealed that the lowest percent disease in...

Gadeeyya G and Ratna Kumar P.K.

...n of parasitized weed propagules like infected leaves, stems, root and floral parts on different fungal growth media. The diagnostic characteristic features of each isolate were critically studied using relevant literature. The pathogenicity of the isolates was examined to select biocontrol agent against host weed. 
...

Wesam Hasan Mohamed Mady1*, Bing Liu2, Dong Huang2, Abdel Satar Arafa1, Mohamed Khalifa Hassan1, Mona Mehrez Aly1, Pucheng Chen2, Yongping Jiang2* and Hualan Chen2

Furqan Sabir1, Muhammad Tayyab1,*, Bushra Muneer2, Abu Saeed Hashmi1, Ali Raza Awan1, Naeem Rashid3, Muhammad Wasim1 and Sehrish Firyal1
Amtul Jamil Sami1,*, Madeeha Khalid1, Rehman Shehzad1, Sana Mughal1 and A.R. Shakoori2 

Riaz Alam* and Muhammad Sajid 

...ars regarding asexual propagation through air-layerage. The daughter saplings of cultivar Manzanilla took less number of days (47.94) to root appearance, produced more rooting percentage (38.89%), number of roots (4.31), root length (4.61 cm) and root weight (1.77g) with more number of re-sprouts (3.18) and shoot length (5.28 cm). The time intervals of air layering also significantly influenced the rooting and growth attributes of olive cultivars. Mid August, ...
Amjad Khan1,*, Muhammad Hassan Mushtaq1, Mansur ud Din Ahmad1, Jawad Nazir1, Zahida Fatima2, Asghar Khan3 and Shahid Hussain Farooqi3
Sumaira Zareen1, Syeda Sadaf Zahra2, Ayeza Mehmood3, Muhammad Asadullah4* and Aish Muhammad5

 

... culture protocol for propagation of an endangered medicinal plant Neurada procumbens L. from Cholistan desert in Pakistan. Nodal segments from healthy grown plants were used as explants and cultured on standard Murashige and Skoog (MS) medium supplemented with different concentrations and combinations of benzyl amino purine (BAP) or kinetin for shoot induction and Indole acetic acid (IAA) for primary shoot and Indole Butyric acid (IBA) for root prolife...

 Shazia Erum, Rashid Anwar and Shahid Masood*

 Ali Raza Shah**, Muhammad Saffar Mirjat**, Abdul Quadir Mughal***, Inayatullah Rajper** and Asif Ali Mirani****

PERFORMANCE EVALUATION OF DIFFERENT TILLAGE SYSTEMS IN A CLAY LOAM SOIL*
...er CT whereas, wheel slippage/travel reduction was significantly minimum (P<0.05) under CTF and maximum under CT. Significantly, higher field capacity was recorded under CTF and lower (P<0.05) under CT. The maximum fuel consumption (P<0.05) was recorded under CT while it was minimum under ZT. Almost similar trends were observed for all parameters in 2012 and 2013. The results suggested that the control traffic system was more efficient tillage system ...
Mingming Ning1,2, Yanjie Zheng1, Yuanyuan Dun1, Weijun Guan2 and Xiuxia Li1,*
... period of subculture propagate fastest from cell cycle examination on Flow Cytometer and growth curve using CCK-8 kit on ELIASA. CAFSCs are induced to neuron and epithelium (epiblast), adipocyte (mesoblast), hepatocyte (entoblast).
...
Nuzhat Huma1, Fozia Ghaffar1, Saima Rafiq2,*, Imran Pasha1, Aysha Sameen1, Imran Hayat2 and Imtiaz Hussain2
Faxiang Wang, Yan Chen, Sai Chen, Xianghong Li, Jian Yu, Jianhui Wang and Yongle Liu*

Muhammad Babar Khawar, Nadeem Sheikh*

Effect of paper industry leachate on various serological indices and serum proteins of wistar rats
...group (P<0.0001). SDS page analysis revealed an overall decreased expression of various proteins in both experimental groups. The protein fractions of 317, 212, 178, 134, 89, 74 and 68 KDa were found in Group 1 compared to control (331, 215, 180, 139, 112, 91, 75 and 69 KDa). However, protein fraction of 202, 171, 136 and 91 KDa were present in Group 2 compared to control. These findings confirmed that paper industry leachate is extremely noxious and induce...

Muddasir Hassan Abbasi1, 2, Noor Fatima1, Syed Shahid Imran Bukhari1, Asma Rashid khan3, Nadeem Sheikh2*

Variations in proteins and transaminases following experimental induction of Bisphenol A in mice
Muhammad Muddassir Ali1,2 and İbrahim Hakkı Ciğerci1,*
...rved for their proper propagation. Thermposis turcica is endemic to Turkey. It’s general anti-oxidant and anti-cancerous activities are explored, but no study has been observed on liver cancerous cell line in term of its genotoxicty. So, genotoxic evaluation was carried out for the alcoholic and hexane extracts of T. turcica. Methanol extracts showed the highest DNA damage (20±1) at 200 µg/ml concentration and 11.67±2.52...

Muhammad Aslam Rajput1,3, Imtiaz Ahmed Khan2, Rehana Naz Syed3 and Abdul Mubeen Lodhi3* 

...ing a large number of propagative materials; standardized germplasm screening protocols as well as optimized inoculation techniques are required. The pathogen inoculation method should be less cumbersome, simple, and rapid. Here, we compared the effectiveness of six different inoculation methods, i.e., dipping, paste, wound+paste, soil infestation, spraying and injection methods for the establishment of smut disease. It appears that disease expression was sign...
Aisha Khalid1, Muhammad Tayyab1,*, Abdual Rauf Shakoori2, Abu Saeed Hashmi1, Tahir Yaqub3, Ali Raza Awan1, Muhammad Wasim1, Sehrish Firyal1, Zaheer Hussain4 and Munir Ahmad5
Naveed Ahmad1,*, P.J.A. Siddiqui1, Amjad Ali1, Khan Mir Khan2, Rafaqat Masroor3, Noor ul Akbar4, Muhammad Amin5 and Mohammad Attaullah6
...wfin seabream, Acanthopagrus arabicus. Five semi-purified experimental diets were formulated containing 350 (P30), 400 (P35), 450 (P40), 500 (P45) and 550 (P50) grams of protein kg-1 of dry matter. Thirty healthy fish (20.94±0.81g initial weight) were stocked in each floating net cage (1.5m x 1.5m x 1.5m), with three replicates of each treatment. The diets were fed to visual apparent satiation two times a day for a period of eight week...

Arshad Farooq* and Muhammad Zafarullah Khan 

...igation application, stoppage of irrigation before harvesting and cane cutting whereas farmers of district Mardan had low knowledge gap in length of cane setts, sowing practices, irrigation and cane cutting techniques. Medium knowledge gap (21 to 50%) was observed in length of cane setts, row to row spacing in farmers of D.I. Khan district while in farmers of Mardan district medium know...

Mehboob Arslan* and Xin Yang 

...l, proliferation, and propagation inside the host cell, viruses modulate certain metabolic pathways to destabilize the host metabolism. These alterations in host metabolic signaling as a result of virus-host interaction generate a favorable environment for the viral pathogens. This phenomenon is a hotspot for investigations in order to improve the diagnosis and drug development of future antiviral therapeutics. In fact, metabolism is considered as a crucial pl...
Sagir Hussain, Erum Iqbal* and Shahina Fayyaz
...ential to survive and propagate proficiently at low temperature (-2 to -15ºC).
...
Naheed Ikram and Nafisa Shoaib*
... marine fishes Acanthopagrus sp. Parastromateus niger, Nemipterus sp., Pampus argenteusIlisha sp., Alepes djedabaEpinephelus sp., Teraponjarbua, Terapon puta, Scomberomorus koreanus, Epinephelus coioides, Lutjanus sp., Pomadasys sp. and Lutjanus johnii procuredfrom Kemari fish harbor (Pakistan). Our study shows that fresh fishes were not contaminated by ...

Masood Ahmad* and Abdur Rab 

...iolus is commercially propagated by corms. The quality and health of corm is one of the important factors that affect the production and quality of gladiolus florets and spikes as well as corm production. Keeping in view, the significant role of calcium in plants and its potential effects on vegetative attributes and quality production of gladiolus corms, an experiment was conducted during the years 2016-2017. The influence of foliar calcium application was in...
Yating Cheng1, Wenlong Shi1, Xue Xiao2, Qirong Zhang2, Qihao Zhang1, Zhijian Su1, Qi Xiang1,2* and Yadong Huang1,2
Qaisar Jamal*, Akram Shah, Syed Basit Rasheed and Muhammad Adnan
...oted. Acquisition and propagation of axenic amastigotes was assessed. During promastigote culture, log, mid-log, and late-log phases were observed respectively on day 4, 5 and 6. The stationary phase was observed on day 7. The day following inoculation, most of the promastigotes were nectomonads having long and slender bodies with roughly uniform width from anterior to near posterior end, having as long flagella as one and a half to twice the body. The nectomo...
Doulat Khan1, Hamayun Khan1, Nazir Ahmad2, Muhammad Tarique Tunio3, Muhammad Tahir2, Muhammad Saleem Khan2 and Rifat Ullah Khan1*

Amna Qazi1, Ghulam Shah Nizamani2*, Muhammad Tahir Khan2, Shafquat Yasmeen2, Shahla Karim Baloch1, Muharram Ali1, Imtiaz Ahmed Khan2, Sagheer Ahmad3, Muhammad Rashid Nizamani4 and Mohammad Aquil Siddiqui

...align: justify;">Micropropagation is a phenomenal technique to serve rapid multiplication of vegetatively propagated plants through in vitro culture. This study was conducted to establish the optimal concentrations of plant growth regulators and sucrose for micropropagation of sugarcane. Sugarcane is cultivated through cane sets and therefore, multiplication of any new genotype throug...
Housh Muhammad Solangi1, Javaid Ali Gadahi1*, Mansoor Tariq2, Bachal Bhutto1Zubair Ahmed Laghari1, Jamila Soomro3, Taufeeq Ahhmed Khosa1 and Abdullah G Arijo1

Muhammad Amir, Syed Waqar Shah, Michael J. Pont 

FAULT-MANAGEMENT ON SYSTEM’S LEVEL IN CAN-BASED “SHARED-CLOCK” ENVIRONMENTS
...olume 37, 2013, issue 1, pages 9-23) which was concerned with fault-management on embedded level. In this paper, we intend to show that the use of a Port Guardian (PG) mechanism can also improve fault-management on system’s level. 

...

 Muhammad Amir*1, Syed Waqar Shah1, Michael J. Pont2

FAULT-MANAGEMENT ON SYSTEM’S LEVEL IN CONTROLLER AREA NETWORK BASED “SHARED-CLOCK” ENVIRONMENTS
...olume 37, 2013, issue 1, pages 9-23). In this paper, we intend to
show that the use of a Port Guardian (PG) mechanism can also improve fault-management on system’s level.
...

Feroza Arshad*, Aftab Ahmed Memon**, Muhammad Aslam Uqaili** and Arshad Habib Malik*

SYNTHESIS OF A NOVEL ROBUST INTELLIGENT CASCADED REACTIVITY CONTROLLER FOR CANDU REACTOR
...d using Adaptive Back Propagation Feedforward Neural Network (ABPFNN). The proposed controller is synthesized in a distributed parallel computing environment using MATLAB. The proposed NICRC is formulated in a highly complex multi-objective neural form and evaluated against full power operation of CANDU nuclear power plant from cold start-up to high power. The performance of highly robust NICRC is tested and evaluated for a typical transien...
Muhammad Shaheen
APPLICATION OF LABELLED K MEANS CLUSTERING FOR GIS CONTRACT AUTOMATION
...alized database. Back Propagation Neural Network (BPNN) is used to train the system on performance indicators defined for compliance by both contracting parties.

 

...

Mohammad Enamul Hoque Kayesh1,2*, Mohammad Tufazzal Hussan3, Md. Abul Hashem2,4, Mohammad Eliyas5 and A.K.M. Mostafa Anower1

Lumpy Skin Disease Virus Infection: An Emerging Threat to Cattle Health in Bangladesh
...are favorable for the propagation of vectors, which should influence the rapid transmission of LSD in the cattle population of Bangladesh. Therefore, LSD may appear as a big challenge to cattle health in Bangladesh in the near future. Consequently, it is very important to take strategic plans and immediate control measures for LSD outbreak control and prevention in Bangladesh. In this review article, we will focus on epidemiology and modes of LSDV transmission...

Kecheng Zhu1,2,3, Peiying He1, Baosuo Liu1,2,3, Huayang Guo1,2,3, Nan Zhang1,2,3, Liang Guo1,2,3, Shigui Jiang1,2,3 and Dianchang Zhang1,2,3,* 

...al analysis of Acanthopagrus latus myomaker (Almyomaker) were performed. The open reading frame (ORF) sequence of Almyomaker is 858 bp, which encodes a polypeptide of 285 amino acids. Moreover, phylogenetic and gene structure analysis indicates that Almyomaker is highly conserved among vertebrates. The tissue distribution pattern shows that Almyomaker is more highly expressed in white muscle than in other tissues. Furthermore, to ex...

Agustine Christela Melviana1, Rizkita Rachmi Esyanti1*, Roy Hendroko Setyobudi2, Maizirwan Mel3, Praptiningsih Gamawati Adinurani4 and Juris Burlakovs5

Gene Expression Related to Steviol Glycoside Synthesis Produced in Stevia rebaudiana (Bert.) Shoot Culture Induced with High Far-Red LED Light in TIS RITA® Bioreactor System
...the hindrances in the propagation of Stevia plants using in vivo methods, an alternative technique of propagation using in vitro methods is needed. The micropropagation method with the RITA® (Recipient for Automated Temporary Immersion System) is used in the production of a large amount of stevia biomass in approximately a short period. The forming of flowers is one of the limiting fac...

Mahmoud Mohamed Ahmed Youssef* and Suzan Abd-Elazeim Hassabo

The Role of Genetic Engineering in Management of Plant Parasitic Nematodes with Emphasis on Root-Knot Nematodes: A Review
... capable of continued propagation. Azotobacter spp. with nematicidal activity could control root-knot nematode, Meloidogyne incognita. The development of such strains by genetic manipulation by transferring gene (s) required for saponins production is effective in increasing crop yield together with fixing nitrogen. Escherchia coli is still used as a host for the recombinant DNA technology – based industrial production of proteins and peptides to manage ...
Arifa Khan1*, Arsalan Bashir1, Shazia Erum2, Jabar Zaman Khan Khatak1 and Aish Muhammad3
Effects of 6-Benzylaminopurine and Indole-3-acetic Acid on Growth and Root Development of Banana Explants in Micropropagation
...banana explants micro-propagated in vitro. Explants were obtained from young suckers of 8818-william, Pisang and Brazilian varieties. The explants were cultured in vitro containing MS media and different BAP and IAA combinations. The results showed banana varieties exhibited differences for the shoot and root development and also for the number of shoots and leaves. Wiallium-8818 hybrid gave more shoots (7.33) when the concentration of BAP and IAA was 2.5 and ...

Noor Ayaz1*, Faiza Aman1, Sidra Saleem1*, Maqbool Rehman1 and Muhammad Fahim2

Olive Cuttings as Affected by Different Concentrations of Indole Butyric Acid
...e used effectively as propagating material for olive cuttings.

 
...

Inam Ul Haq1*, Humara Umar2, Naeem Akhtar3, Muhammad Azhar Iqbal2 and Muhammad Ijaz1

Techniques for Micropropagation of Olive (Olea europaea L.): A Systematic Review
...align: justify;">Micropropagation is one of the most significant applications of biotechnology in the field of horticulture. This technique is used for proliferation of olive (Olea europaea L.) tree which is globally cultivated for centuries and its value added products are consumed worldwide. The traditional propagation techniques of olive are uneconomical with wastage of time while micropropag

Muhammad Ihtisham1, Noor Amjad2, Muhammad Nauman3, Asghar Ali3, Khawar Riaz3, Muhammad Sajid3, Muhammad Owais Shahid*3 

 

...n and cheap source of propagation. However, several plant species have water-impermeable hard seeds which take a long time to germinate. The hard seed coat can be scarified successfully through acid treatment. Sophora is a beautiful ornamental plant and its seeds have the same issue. In this experiment, seeds of Sophora secondiflora were treated with H2SO4 and then sown at different dates. Both H2SO4 scarification and sowing time had a significant effect on ge...
Javaria Sherani1,2*, Muhammad Saleem Jilani1, Tanveer Ahmad2, Sohail Kamaran3, Abdul Manan2, Tehseen Ali Jilani2 and Mateen Sajid2
 
... should be used to be propagated on local rootstocks to enhance yield as well as fruit quality that will contribute in ensuring food security.
...

Anwar Ali1,5*, Horia Shaukat2, Munir Ahmed3,5, Ahmed Bostani4 and Syed Ashiq Hussain3

...imulation fastens the propagation and termination of rigor mortis hence decreasing the processing time and fatigue. After electrical stimulation, the muscles become paralyzed and the rigor mortis is dispatched to reduce the time spent in the muscle and his fatigue. It is also found to enhance meat tenderness and to boost meat sensory attributes. But it may also have adverse effects on some meat quality characteristics like holding capacity of water and other o...

Salma Javed and Samreen Khan*

...y. On the first week, propagation rate was noted approximately 2.5x10³ followed by 3.9x10³ on the second week and finely after three weeks incubation period the greatest multiplication rate estimated up to 8.9x10³; however, the same did not appeared on Aspergillus niger and result was found negative. The main purpose of captioned study was to report mass culturing of parasitic Aphelenchus avenae and their relationship and further explore these n...

Talat Farid Ahmed1*, Shamim-ul-Sibtain Shah1, Ashfaq Ahmed Sheikh2, Hashim Nisar Hashmi3, Muhammad Atiqullah Khan1 and Muhammad Azeem Afzal1

...rastructure, without stoppage of Canal breaches, minimized damages to standing crops in command area, and will ensure improved irrigation supplies in the Koh e Suleman (Pachad) area, enhancement in economic condition of the area, stable farming in the Pachad area and providing maximum use of flood water for agriculture to increase production and ultimately for socio-economic uplift of the area. The data regarding flood levels and topographic maps have collecte...

Arshad Khan1, Mohammad Ihsan1, Mohammad Nisar1, Ali Hazrat1*, Murad Ali3, Rashid Ul-Haq3, Khalid Khan2, Karishma Gul1 and Shah Faisal1 

Tahseen Ullah* and Noorul Amin

...s recommended for the propagation of Berberis lycium from cutting.

...

Fahrauk Faramayuda1,2*, Totik Sri Mariani3, Elfahmi1,4 and Sukrasno1

...nds abundantly and to propagate purple and white-purple varieties of O. aristatus. A strategy that can be adopted  to achieve plant tissue culture (callus induction). This research aim is to identify secondary metabolite in callus using thin layer chromatography (TLC). The profiling of ethanol extracts of callus of two varieties O. aristatus were carried out using solvent system toluene-ethyl acetate- formic acid-water (3:3:1:0.2).  The results of mo...
Asmaa A.M. Abd El-Ghani *; Ahmed B.M.; Abdel-Raouf A. ; Nagwa S. Ata* and H.A. Hussein
...) were used for virus propagation and titration. The
effects of two concentrations of Salvadora persica on viral growth in MDBK cells as
well as cytolytic activity of BHV-1 were evaluated at different times pretreatment
,simultaneouslly and after adsorption. The results demonstrated that the safest
concentration (non-cytotoxic) of the plant extract under study on MDBK cells was 0.1
mg/ml.The plan extr...

Sherif .M. Ibrahim*, Abd El-Razek. B. Abd El-Razek*. Hanan. M. El-Zahed Amal. A. Fatouh* and Ayatollah. I. Ibrahim*

...isolated strains were propagated on the chorioallentioc membrane of specific pathogen free (SPF) embroynated chicken egg (ECE) showing the characteristic pock lesions for each strain with titers reached 4.5 and 3.5 log10 EID50/ml at 5th passage for FPV and PPV isolates, respectively. The isolated strains were further identified using the specific hyper-immune sera for each virus by virus neutrization test (VNT). As the Neutrization index (NI) for PPV and FPV i...
Abd El-Hamid, M.I1, Seham, A.El-Zeedy1, El-Sanousi, A.A2, Reda, I.M2, Nehal, S. Saleh1, Abbas, A.M1
...ain the stability and propagation of the cloned gene then the cloned plasmid isolated to be transformed again in Ecoli BL21 cell which include the T7 promotor for expressing the glycoprotein D, western blotting carried out on the induced culture at different expression times using the alkaline phosphatase labeled N-terminal anti histidine monoclonal antibodies revealed single bands at the expected size, the same samples have been reacted with serum collected f...

Ahmed A. Azab, Wesam H. Mady, Ali Zanaty, Mahmoud Samir

...lts revealed that the propagated virus in CEK showed the highest virus titer (5.952 x
106 and 3.245 x 106) than Vero cell line (7.184 x 101 and 2.14 x 103) and ECE (7.536 x 103 and 5.444 x
105) after both 48 and 72 hours respectively.
Conclusion: The chicken embryo kidney cell (CEK) is the most successful and sensitive monolayer
cell culture host system for isolation of the Egyptian variant 2 strain of infectious...

Maha A. El-Abhar1, Moustafa A. Elkady1, Khaled M. Ghanem2, Hussieny A. Bosila3

...irus isolate was then propagated in
potato Ditta cv. plants, The virus was identified on the bases of host range, symptomatology,
transmission and serological diagnosis, in addition to the ultrastructural changes produced in potato
leaf cells infected with AMV .
Results: Reaction of thirteen plant species and cultivars belonging to four families (Amaranthaceae,
Solanaceae, Fabaceae and Laminaceae) to ...

Mohsen M. Elsharkawy1, Salem H. Homayed1, Mohamed M. Elsawy2 and Amr A. Khedr1

...
pathogen propagation and disease development.
...

Ahmed M. Soliman

...ium amaranticolor and propagated in healthy Nicotiana benthamiana. Enzyme linked immunosorbent assay (ELISA) and reverse transcription-polymerase chain reaction (RT-PCR) with specific primers designed for the coat protein gene of CMV were used to detect the virus. RT-PCR products (657 bp) of coat protein gene of CMV were cloned and then sequenced.
Results: CMV was isolated from four vegetable crops (cucumber, tomato, pepper and watermelon) using EL...

Youssef A. Elgharbawy1, Mervat M. Ali1, Ayman H. El-Deeb2, Mohamed A. Shalaby2

...ia isolate of LSD was propagated and titrated on Madin Darby Bovine Kidney Cells (MDBK) till reach the highest titer (10 5.5 TCID50/ml). The inactivation process was performed using Bromoethylamine hydro bromide (BEI) that did not affect the immunogenic characterization of the LSDV and the process last for 18 hours with 1% concentration of BEI at 37°c. The inactivated LSDV tested on MDBK cells and the animals free of antibodies for residual live virus and ...
Hadeer M. Mossa, Ausama A. Yousif, Emad A. Aboelsoud, Mahmoud El Gamal, Ahmed El-
Sanousi
...te of LSDV strain was propagated on MDBK cell line and CPE was appeared
after 72 hours as cell rounding, cell aggregation and cell degeneration with titer of 105.5TCID50/ml,
DNA extraction and PCR for the candidate strain was performed using universal and specific primers
for virus identification and confirmation. The size of the produced amplicons was 192 pb for the
universal primer and 502pb, 1452pb for the spe...

Mohamed M. Mashaly1, Ayman H. El-Deeb2, Momtaz A. Shahein1, Hussein A. Hussein2

...ceptible cell for the propagation of LSDV with best harvesting time
72hr P.I to obtain a maximum titer for subsequent vaccine production.
...

Muhammad Rafique1, Muhammad Azhar Iqbal2, Inam Ul Haq1*, Muhammad Ramzan Anser2, Humara Umar2 and Muhammad Ashraf Sumrah2

...ed out to standardize propagation technology concerning optimization of light intensity under greenhouse conditions. The current study was conducted at Izhar farms (Pvt.) Ltd. under the collaboration with Barani Agricultural Research Institute (BARI) Chakwal to find out the appropriate light intensity for successful olive propagation under greenhouse conditions. The data regarding days to root initiation, number of roots, ro...

Mazhar Ullah and Mohammad Sayyar Khan*

...r the effective micro-propagation of sugarcane. Various factors affecting tissue culture must be evaluated and optimized to produce maximum callus and regenerated plantlets. In this research, different auxins [Dichlorophenoxy Acetic acid (2,4-D), Nephthalene Acetic Acid (NAA)], cytokinin [Benzyl Amino Purine (BAP)] either alone or in combination with each other and carbon sources [sucrose, glucose and fructose] with varying concentrations (2, 4 and 6%) were ev...
Muhammad Taqi1, Shazia Erum2, Shamaila Rasheed2, Sadar Uddin Siddiqui2 and Shakeel Ahmad Jatoi2*
... large-scale and fast propagation of C. tuberculata through in-vitro organogenesis was carried out at in-vitro lab, Bio-resources Conservation Institute, NARC, Islamabad, Pakistan. Nodal-tips as explants were grown on MS salts enriched with diverse plant growth regulators including BAP, NAA, Kin and 2,4-D, alone as well as in combinations. The highest growth response for callusing 78.3% was observed in BAP (0.5mg/L) in combination with Kinetin (0.25mg/L) +NAA ...

Vahid Mollasadeghi1,2* and Samaneh Elyasi1,2

...ings of potatoes were propagated and grown and then transferred to sterile plastic pots with suitable soil. The randomized complete block design (RCBD) was performed in three replications. Pars Humic treatment (Ammonium Humate) was treated with control concentrations (zero), 1, 2 and 3 per thousand. PVY-activated tobacco leaf extract was used to infect the potato plant. This paper measured chlorophyll a, b, leaf area, number of leaves, stem length and tuber we...
Johar Hussian1, Faiz Muhammad2, Shafqat Fatimah Rehmani3, Aqeel Ahmad4, Nazir Ahmad Lone4, Moomal Bughio5, Syed Khurram Freed1 and Shakeel Ahmed Khan4*
Furqan Awan1,2, Sadia Nawaz3, Muhammad Muddassir Ali3,*, Asia Iqbal4, Raheela Akhtar5, Sehrish Firyal3, Haleema Sadia6, Arshad Javid4, Muhammad Rashid7, Muhammad Sulman Ali Taseer8 and Zhenling Zeng1
Aqsa Fayyaz1, Muhammad Kashif Zahoor1*, Asma Ashraf2, Muhammad Asif Zahoor3, Azhar Rasul2, Humara Naz Majeed4, Muhammad Zulhussnain1, Kanwal Ranian1, Bushra Riaz1, Nazia Khalil1 and Attaullah1
...nated chicken eggs to propagate the NDV, which was then titrated using haemagglutination inhibition assay for quantification of NDV. Polyclonal antibodies were produced in experimental rabbits followed by titration. These antibodies were then used to detect the presence of NDV. Sixteen samples were found positive (20%), 62 negative (77.5 %) and two unreactive (2.5 %) for NDV. All of the poultry farms around Faisalabad showed positive NDV (ranging from 1 to 4 s...
Syarif Husen1*, Devi Dwi Siskawardani2, Setiawan Deny Rexmardi1, Erny Ishartati1, Muhidin Muhidin1, Jumpen Onthong3 and Ivar Zekker4
...gmediacompositiononthepropagationofstemcuttingpotato(G0)plantedinthe screenhouse.ConductedinSumberBrantasVillage(1700ma.s.l.),BumiajiRegency,City ofBatu,EastJava,Indonesia,thisresearchutilizedRandomizedCompleteBlockDesign withsevengrowingmediacompositionsandfourreplicatesinamicro-cuttingplanting.The growingmixmediacompositioncontainedhuskcharcoal+cocopeat+bokashi(goat manure) + bokashi(cowmanure), andhuskcharcoal+ cocopeat+bokashi(cow manure) + bokashi(poultry...

Mirtneh Akalu1,2*, Takele Abayneh2, Esayas Gelaye2, Behailu Tefera2, Teferi Degefa2, Vemulapati Bhadra Murthy1 

Mubasshir Sohail*, Qadeer Ahmed Soomro, Raza Muhammad, Muhammad Usman Asif and Imran Rauf

Sania Subhan Qureshi*,1, Baitullah Khan1, Shahid Khan1, Hanif Ur Rahman1 and Muhammad Subhan Qureshi2

...study was designed to propagate the virus on BHK-21 cell line. The results revealed that serotype ‘A’ virus virulence is more as compared to serotype ‘O’ and ‘Asia-1’. Although less virulence difference was found between serotype ‘A’ and ‘O’. Serotype ‘Asia-1’ was comparatively less virulent. Randomized Complete Block Design (RCBD) was used for determining the effect of incubation time, vi...

Tariq Mahmood1*, Shakeela Ismail1, Faraz Akrim2, Muhammad Farooq1, Nadeem Munawar1 and Muhammad Raza Khan1

Amjed Ali1, Muhammad Tayyab1*, Abu Saeed Hashmi2, Asif Nadeem3, Shumaila Hanif4, Sehrish Firyal1, Shagufta Saeed1, Ali Raza Awan1 and Muhammad Wasim1

Xiangli Dong1,2, Shilin Mikhail Borisovich2, Jiji Li1,*, Jianyu He1, Zeqin Fu1, Yingying Ye1, Julia N. Lukina3, Olga V. Apalikova4 and Jianshe Zhang1

Anara Ryskeldina, Indira Iskakova, Nurgul Sarina , Alexander Shevtsov , Laura Syzdykova, Alexander Shustov, Yerlan Ramankulov, Marat Kuibagarov* 

Iram Amin1,2*, Shazia Rafique1, Nadeem Ahmed1, Muhammad Shahid1, Samia Afzal1, Tahir Rehman Samiullah1, Mohsin Ahmed Khan1 and Muhammad Idrees1,3

Hanaa H.A. Gomaa1*, Dalia Y.Z. Amin1, Mona A. Ismail1 and Khalid A. El-Dougdoug2

...a glutinosa for virus propagation. Fresh wood cuttings (4-6 nodes) were collected from naturally infected fig plants. Healthy fig plants were grafting-inoculated with chip budding of infected fig plants and kept in a greenhouse. Symptoms were confirmed by DAS-ELISA. Strips of infected and healthy N. glutinosa leaves were viewed under light microscope. Extracted infectious sap of N. glutinosa leaves was examined using Transmission Electron Microscope. Total RNA...

Saydat Saad Abd El-Megeed1, Walaa Yehia El-Sayed1*, Tarek Khamis2 

...Ps in combination with Empagliflozin suggestively declined the blood glucose levels, lipid profile, and liver and kidney functions. A reduction was recorded in malondialdehyde (MDA), as well as improved catalase (CAT), glutathione peroxidase (GPx), and superoxide dismutase (SOD). A reduction was found in the gene expression in the hepatic homogenate of Patched 1, hedgehog-interacting protein (Hhip-1), smoothened (SMO), glioma-associated oncogene homolog 1 (GLI...

Saba Rehman1, Faisal Salih Hayat1, Sadia Norin2, Abdul Aziz1, Siddiq Ur Rahman1* and Noor ul Haq1*

Nauman Zaheer Ghumman, Muhammad Ijaz*, Arslan Ahmed, Muhammad Umar Javed, Iqra Muzammil and Ahmed Raza

Saima Mumtaz1,3*, M. Imran Kasana1, Riaz Alam1, Noorullah Khan1, Muhammad Noman1, Sanjeela Sabahat1 and Hussain Shah2

... chinensis) is mostly propagated through air layering which is cumbersome because of low survival rate of layered plants, more requirement of mother stock and labor intensive method. To address these issues an alternative technique has been employed for quick multiplication of litchi. In the present study we have investigated the influence of various levels of Indole Butyric Acid (IBA) on four Litchi cultivars (Gola, Surahi, China and Bedana) for root inductio...
Aadil Sultan, Mohsin Ahmad Khan*, Nadeem Ahmed, Muhammad Hassan, Rashid Bhatti, Hafsa Naeem, Muhammad Islam Khan, Saad Tahir,  Samia Afzal* and Ahmad Ali Shahid

Imran Muhammad Siddique1, Naseem Sharif1*, Abdul Ghaffar2, Mudassar Naseer1, Ammara Noreen3, Naseem Akhtar4 and Safeer ud Din5

... vegetative method of propagation thereby avoiding inevitable heterozygosity and polyembyonic nature of citrus fruits.

...
M. A. Abou El- Nasr1, Kh. A. Dougdoug1, Hayam S. Abdelkader2, and Rehab A.
Dawoud2
...ar apricot trees, was propagated on apricot healthy seedlings. Degenerated oligonucleotide primers were designed to amplify the N-terminal portion of the capsid protein of PPV. The amplified products were cloned into pGEM-T-Easy vector and hybridized to PPV DNA specific probe labeled with Dig-I 1dUTP. DNA sequencing using fluorescent dideoxy nucleotides showed that the capsid protein region of PPV-EA strain had about 65% sequence homology with other strains of...

A.S. Gamal El din1; Sohair I. El-Afifi2; A. S. Sadik 2; Nashwa M. A. Abd El-Mohsen1; and H. M. Abdelmaksoud1

...ector by ligation and propagation after transformation process in E. coli. (2) Isolation of plasmid DNA. then used restriction enzymes Bam HI and Bgl II to identify clones containing inserts. To confirm the fragment inserted of CP gene sequence. PCR was performed using specific primers for PVYN CP gene. (3) The gene expression was performed using 100 µM IPTG & 2 µM biotin and incubation for 4h / 37 oC to produce biotinylated protein for E. coli...

E. K. Allam1; Kh. A. El-Dougdoug1; M. A. Amer2; A. A. Abou-Zaid2; and S. M. Amin2

S.A. El-Kewey1; R.A. Omar1; S.A. Sidaros2 and Samaa Abd El-Khalik3

...on through vegetative propagation reached 100% in both cultivars (Chinese and Balady) with different degrees of symptoms severity. Plant height, leaf area. fresh and dry weight of bulb decreased significantly with the increasing of severity symptoms on garlic plants of both cultivars. The electron microscope preparations of crude sap extracted from naturally infected garlic plants and negatively stained with 2% PTA showed flexuous rod-shaped particles 657-714 ...

Asim Faraz1, Amal AlKharusi2, Nasir Ali Tauqir3, Muhammad Shahid Nabeel4, Syeda Maryam Hussain5, Abdul Waheed1*, Muhammad Arslan Akbar6

...Mahni for rearing and propagation during period 2014-2016. The farm management worked to improve and propagate the breed of Thalli Sheep. Different management practices like identification, weighing, vaccination, deworming, dipping, spraying, hoof trimming, shearing, breeding, lab screening and weather management were practiced to increase the fertility and decrease the mortality percentage. Average birth weight was improved...

Muhammad Akram¹,²*, Rashid Mahmood³, Fahim Arshad4 and Umer Farooq Gohar5

...que to develop a mass propagation system for the effective multiplication and conservation of P. minima. Juvenile leaves were cultured on MS basal medium supplemented with 0.1, 0.5, 1.0, 2.0, and 5.0 µM thidiazuron (TDZ). As a result, 100% callus induction was achieved on the medium containing 1.0 µM TDZ. Notably, calli grown on 1.0 µM TDZ exhibited greater potential for shoot regeneration and proliferation in the growth regulator-free MS liq...
A.l. Abd El-Fattah; A.s. Saclik M.M. El-Kholi; I.A. Åbdcl-Hmnid and M.A. Madkour

Atena Azarniush1, Majid Moghbeli2*, Farshid Kafilzadeh1, Mohammad Kargar1 and Hooshang Jamali1

Anwar ul Haq1, Maqsooda Parveen2, Shehzadi Saima3* and Khalid Masood Ahmad1*

...ters in chrysanthemum propagated by means of tip cuttings.

...

Munawar Lal1*, Naeem Tariq Narejo2, Muhammad Hanif Chandio1, Faheem Saddar3, Hameeda Narejo4, Ghulam Dastagir5, Ghulam Abbas6 and Shahnaz Rashid6

...7.0 cm in length. Acanthopagrus latus had a total population of 185 individuals, measuring 3.2-20.2 cm in length and 3.56-168.86 g in weight. Arius thalassinus was 210 and ranged in length and weight from 5.4 to 19.8 cm and 4.32 to 88.9 g, respectively. In comparison to the other four dominating species from Nurri Lake, District Badin, Sindh, Liza subviridis, Aulopaereiaocellata, Acanthopagrus latus, and Arius thalasinus spe...

Javeria Khourshid*, Ghulam Abbas, Shahnaz Rashid, Asma Fatima* and Abdul Malik

...mis niloticus and Acanthopagrus berda) were used for this study and kept in two different treatment tanks with their replicates (n=10 each) designated as T1= OxyAqua and T2= simple aeration. Juveniles were fed on supplementary pelleted diet thrice daily at 2% of their total body weight. Results showed that WG and SGR were significantly higher in T1, in which we applied Oxy Aqua organic® product without aeration than T2, whereas no significant difference (p...

Yanping Li, Fei Xu, Yongming Wang*, Yunyun Lv, Jinrong Shi, Biwen Xie, Wenyao Cai and Dian Liu

... to implement captive propagation programs to protect their populations. Here, we firstly carried out artificial breeding of S. superciliaris and S. reevesae, and hybrid combinations were designed as S. superciliaris ♀ × S. reevesae ♂, and S. superciliaris ♂ × S. reevesae ♀. Secondly, 19 microsatellite loci were used to evaluate genetic diversity between hybrid offspring and their parents. The mean polymorphic information content (PIC) amon...
Tariq Ahmad1*, Anum Razzaq2*, Li Bo1, Faiz ur Rehman3, Gul Saba2, Omama Saqib1, Saif Ullah2 and Muhammad Suliman1

Sajid Ali1, Ghulam Abbas1, Shahnaz Rashid1, Asma Fatima1*, Abdul Malik1, Dilawer Ali1, Muneer Hussain Bijoro1, Ushra Batool Hashmi1, Rumaisa Abdul Rahim1, Shahid Hussain2, Kashif Ali3, Jabbar Memon4 and Javeria Khourshid1

...lackfin seabream, Acanthopagrus berda (Forsskal, 1775) by using different protein-containing diet i.e., soybean meal and fishmeal. The dietary efficacy was monitored in seawater earthen ponds designated into four nylon meshed hapa (3.7×9.5×9.5 feet) for 60 days. Juveniles (35.75±3.2g) were collected with the help of cast net from Sakro creek and transferred into the earthen fish ponds located at Garho, Thatta, Sindh. Juveniles were acclimati...

Nasratullah Baloch1, Naeem Tariq Narejo2, Hamida Narejo3, Muhammad Farooq Hassan4, Ghulam Abbas5, Shahnaz Rashid5, Muhammad Hanif Chandio6, Faheem Saddar7 and Majida Parveen Narejo8

...lusmn;0.4 cm) and Acanthopagrus berda (7.99%; TL 1.47-28.5± 0.5 cm). The Simpson’s biodiversity index (1-D=0.223) shows that the Sonmiani water noted as low in terms of fish diversity, growth and propagation. Length-weight relationship of four dominant species was calculated as follows. Liza subviridis: Log a -0.23+2.20, Sardinella longicep: Log a -0.78+2.92. Silago sihama: Log a -0.70+2.20, Acantho

Saad Tahir, Nadeem Ahmed*, Mohsin Ahmad Khan, Muhammad Akram, Rabia Abbas and Kausar Malik

Abdul Latif Rind1*, Shahid Hussain Abro1, Rameez Raja Kaleri2,3, Ghulam Mustafa Solangi4, Raza Ali Mangi5, Muhammad Anees Memon6, Depeesh Kumar Bhuptani7, Sana Noor8, Zainab Lanjar9, Zahid Ali Mangrio10 and Abdul Wahid Solangi

... measures to curb its propagation.

...
El-Dougdoug. K.A.1, Mervat, M. Fath Alah2, Reham A. Hassen3,Rehab A . Dawoud2
...robial of potato micropropagative in vitro. The occurrence of induced resistance and increased potato plantlets were found by MS medium treated with culture filtrates based on reduction of PVY infection, disease severity, biochemical changes accompanied to induced resistance (the high level of endogenous salicylic acid, protein content, chlorophyll contents and peroxidase and polyphenol oxidase activities as well as
Nassarl , Entsar A.; El-Dougdoug , Kh. A.; Osmanl , M.E; Dawoud3, Rehab
A. and Kinawy l , Aliaa H.*
...y of TMV-Ch-free micropropagated shoots via application of 20 mg/l virazole followed by thermotherapy at 380C for two weeks and early screening to facilitate the efficient production of virus free tissue culture derived propagules using the produced antiserum against TMV-Ch-EG.

...

Aboud*, K. A; Gomaa**, Hanaa H. A.; El—Taholowy***, Mohage, A. and El - Sugher**, S.

...ained virus free micropropagated plantlets were subjected to DAS-ELISA detection. Tissue culture-derived genetic stability banana plants and virus tested plants were screened by RAPD- PCR. Only two RAPD primers (among 10 tested) were chosen as producing polymorphic DNA bands differentiating the investigated micropropagated plants. Based on DNA markers, the genetic stability between micropropag...

Ahmed*, Amal A.; Khatab*, Eman A.H. ; Dawood*, Rehab A. and Ismeil*, Amira .M.

...roliferation and micropropagation of the infected shoot clumps respectively. The plantlets were rooted in MS medium supplemented with (1.5 mg/l NAA ) . Plantlets regenerated from meristem measuring 0.1 mm and 0.2 mm yielded (90 and 80 % ) for CLV and (92 and 80 ) for CarVMV virus-free tested plantlets respectively,while larger meristems (0.3 and 0.4 mm ) were not effective . As well as the plantlets survival was (20 , 35 , 65 and 80% ) for meristem measuring( ...

El-Tabakh, SAA l ; Abdel Wahab, KSE; Badr, AF   and Helal, IG  

Eman, Abo Hatab*; Hussein, HA.; El-Sabagh IM. and Saber, MS.

...ulture harvest of the propagated viruses and RT-PCR using primers specific for VP6 and VP7 of group A rotaviruses was employed and confirmed the molecular characterization Of the isolates viruses with the correct and expected bands. The RT-PCR specific band of VP7 gene of two selected isolates was eluted from the agarose gel and sequenced using VP7 specific primers sequence. The obtained sequence was analyzed using computer software (BLAST) which revealed that...

El-Sabagh, I.M.; Hussein, H.A.; Amer, H.M.; El-Sanousi, A.A.; Reda,I.M. and M.A. Shalaby

Amer, H.M.; Hussein, H. A.; El-Sabagh, I. M.; El-Sanousi, A. A. Saber,M.S. and Shalaby, M. A.

...quences using PCR and propagated for generation of high-titer viral stocks. In vitro expression studies utilizing the recombinant baculoviruses were conducted and revealed high-level expression of N protein as indicated by its distinct reactivity in immunofluoresence, solid phase ELISA and Western blot.

...
Hala M. El-Makaky*•, Sherif, N.A.;Fekria El-Bordiny* and Taha, M.M.*
...SHSV was successfully propagated on the yolk sac of 5-7 days of SPF embryonated chicken eggs. The virus was harvested 48-72 hours post inoculation. The titre of the virus reached 1062 EID50ml. One hundred and fifty chickens were divided into 3 equal groups. The first group was vaccinated with the prepared SHS vaccine, 2nd group two was vaccinated with imported SHS vaccine, while the third group was kept as non-vaccinated control. Evaluation of the prepared vac...

Eman, M. Abd El-Mottaleb.*; Ahmed, S.A."; Maysa, H.M.*; Salem, S.A.* and M. M. El-Shenawy

...or BEFV isolation and propagation on Vero, BHK21 cell lines and baby mice. Identification was done by both Indirect Fluorescence Antibody Technique (IFAT) on tissue culture and inoculated mouse brain, while immunoperoxidase technique was done on bovine lung. Haematological examination revealed leucocytosis accompanied with neutrophilia (immature form) and lymphopenia. Histopathological examination clarified the most common lesion of field cases in lung in the ...

Mayada A. Abd Elgalell; B.A Othman2; Th. Radwan, 1 and Amal S.M. Abo-Sinna3

... PP2. The phages were propagated and purified by polyethylene glycol, 6000-sodium dextran sulfate 500 two phase system. The two phages had different ultraviolet absorption spectra. Results of the lytic pattern of both phages showed that, they had a restricted host range. Particle morphology of the isolated phages was determined and it was found that the two viruses have tadpole shape, which phage PPI has an isometric head (135 nm) and long non-contractile tail...

M. Osman1, Kh. El-Dougdoug2, E. T. Abd El-Salam3, R.M. Taha4 and R.M. El-Hamidl

...olor. CMV-isolate was propagated on squash plants var. Eskandarani. After 15 days. systemic symptoms appeared in the newly formed leaves in the form of vein banding, severe mosaic, yellowing, malformation, and leaves wilting. Histopathological studies of CMV-infected squash leaves showed changes such as compact mesophyll, disintegrated and less lignified metaxylem, slight upward blistering of leaf blade, closed stomata, short palisade and rounded spongy cells ...

B. A. Othman; Kh. A. El-Dougdoug; M. H. Abdel Ghaffar and T. F. El-Arabi

H. A. Hussein1, N.A. Mohamed2, F.M. Mohamed2 and M.A. Shalaby1

...irus titration assay. Propagation of these viruses in BVDV-free cells for five passages showed decrease in the severity of CPE with marked difference in virus titer when compared with those passaged in BVDV infected cells. The present study. confirms that the cytopathogenicity of the local isolates of BVDV are directly related to the presence of a helper virus which could be NCP homologous or heterologous. After 12 passages in cell culture. the obtained BVDVs ...

Rawaa Saladdin Jumaa

...ibroblast (CEF) cells propagated by the prepared infected CAM have shown the cytopathic effect (CPE) that included rounding, detachment of the cells from the monolayer and aggregation of cells during the first and second tissue culture passages. The results of sequencing analysis of pigeon poxvirus field samples revealed that the gene has some mutations (nonsense and missense) and is highly conserved among pigeon poxvirus isolates. The phylogenetic analysis re...

M. Imran Kasana, Rashid Iqbal Khan*, Noor Ullah Khan, M. Noman, Shahid Ali, Saima Mumtaz, Shamaila Rasheed and M. Qamar-Uz-Zaman

...lly, avocado has been propagated through seed production that led to variability among off-spring and long juvenile period. Therefore, asexual propagation via grafting is the most appropriate method for producing high-quality, true to type plants. The current study was planned to evaluate four different grafting techniques (Cleft, Tongue, Patch and T-budding) in ten different cultivars of avocado. The outcomes suggested that...

Shahid Iqbal1, Arshad Khan, Ali Hazrat1*, Gul Rahim, Mohammad Ihsan1, Umar Zad Gul1, Maryam Bibi1, Khadija Bibi1 and Muhammad Mukhtiar2

Aatif Masood Ahmad Khan1, Masood Rabbani1*, Arfan Ahmad1, Muhammad Wasim2 and Sohail Raza1
...e. In the current study, page serotyping demonstrated that all isolated Av. paragallinarum belonged to page serovars B. Among the media evaluated for growth of the isolated strain BHI/SN appeared to be the optimum media supporting the maximum growth rate and providing the maximum cfu count and the highest value of specific growth rate during the exponential phase of bacterial growth curve were obtained at 8 h. Thus the curre...

Muhammad Bilal Zia1*, Ashar Farooq1, Hammad Ud Din1, Barkat Ullah Khan1 and Samee Ud Din2

...al planting stock and propagation methods for Paulownia indicated that the root stumps grew substantially taller, having straight stems and symmetrical crowns. Highest survival was observed for Paulownia fortunei, Paulownia tomentosa and Paulownia elongata. Paulownia fortunei and Populus deltoides; both had comparable growth in diameter and physico-mechanical properties. Apart from its utilization in wood works, its scope is also emerging in pulp and paper ind...

I Ketut Puja1*, I Nyoman Sulabda2, Ni Nyoman Trinayani3, Ni Wayan Patmawati3, Made Rahayu Kusumadewi3, Putu Bulan Sasmita Dewi3, I Wayan Sukernayasa4, Anak Agung Gede Oka Dharmayudha5, Putu Devi Jayanti5, I Wayan Nico Fajar Gunawan5 

TitleVol71(2)2021
... of Vol.71(2), 2021...
Title page and contents of PJF Vol.70(2), 2020
...JF Vol.70(2), 2020....
TitleVol.71(1), 2021
...of Vol.71(1), 2021....
Muhammad Yousaf Khan and Pervez Manan
...us is the most widely propagated tree species throughout the world, because of its adoptability to site, types of management systems, multipurpose nature and fast growth the Eucalyptus was introduced in Sub-Continent in late 18th century for the first time. Need for conducting this study was felt to dig out the facts about Eucalyptus plantations in Malakand District after a protest of few participants organized and brought by NGOs based at Peshawar ...
Muhammad Tahir Laeeq and Muhammad Asif Aziz
...icidal value. Neem is propagated primarily through seed, but seeds are having viability for short period of time. To get healthy and good quality planting stock appropriate nursery techniques are imperative.

The study was initiated to assess the effect of various nursery techniques on the growth improvement of neem seedlings for obtaining healthy planting stock. The study comprised of four experiments including (i) application of fertilizer (urea) @ ...

Mian Muhammad Shafiq and Hussain Bux Bhaagat
...nds are formed by the seepages from Jamrow and Nara canal. A general survey of the area was conducted in the month of January 2006 and direct (field observation) and indirect (meeting with communities and wildlife staff) observation methods were used.

These lakes support a good population of waterfowls, Marsh crocodiles, otters and fish stock. About 1% of global population of globally threatened duck specie i.e. Marbled teal (Marmaronetta angusti...

Mohammad Khan
...oza pine under clonal propagation at par with other fruit crops, efforts were made to graft scion-wood of this species on root stock of chir pine, which has proved successful. This paper presents results of two experiments conducted for the purpose....
Md. Aktar Hossain , Mohammad Kamaluddin and M. Serajuddoula
...f the species. During propagation, cutting morphology is not significantly changed due to the etiolation of the stockplants. ...
Muhammad Afzal, Muhammad Mushtaque, Aqeela Mubeen Akhtar, Nighat Chughtai and Shaheena Ramzan
...d of two months...
Muhammad Afzal
...h known as clump. The propagation of bamboos by seed is most successful but many of the bamboos flower and seed after long intervals. Seeding cycles vary from species to species and may be 30-100 years (Banik, 1980). Due to long inter-seeding intervals, the propagation is done vegetatively. Bamboo cultivation by rhizome planting is the most common method applied in this country. Normally, it takes 10 years for nonclump-formi...
K. M. Siddiqui
... the latter case, the seepage of irrigation water from unlined water courses has aggravated the problem to an enormous and critical extent. Unfortunately, role of forests in this regards is little understood and only mechanical means have been used to reclaim salt affected and waterlogged areas at a great expense by the government agencies such as WAPDA and accepted by the public at large....
Raja Atta Ullah Khan
... private lands. It is propagated with root shoot cuttings. Prevailing technique of raising of shisham nursery consists of broadcasting sowing of shisham pods on raised beds and keeping the beds moist with water through trenches till the seed germinates. Although the seed germinates very profusely and the density of seedlings in good nurseries varies from 100 to 160 per sq. metre of nursery area, yet the average number of standard size planting...
Muhammad Khan
...n Pakistan. It can be propagated from seed and stem or root cuttings. Propagating from stem or root cuttings is important for producing genetically uniform planting stock. The stem cuttings are difficult to propagate as compared to root cuttings. This paper reports results of the study on determination of suitable size of paulownai root for vegetative propag...
Hakim Shah and Malik Illahi Bakhsh
...urpose of sale. The stumpage value of annual wood removals from farmlands is estimated at 5.7 billion rupees. The tree stock is equivalent to 0.73 million ha of plantation forests....
Muhammad Khurshid Swati and Muhammad Afzal Cheema
... including volume of stumpage. The out turn volume can also be expressed as percentage of the standing volume of the tree. In the process of harvesting and conversion into logs or scants a certain proportion of the standing volume is lost as smallwood, Pharas, kurf or left as stump on the ground. The outtern volume of trees in logs or in scants primarily depends on parameters such as species, taper, site quality and density of the crop. At present the...
M. Iqbal Ahmad and I. A. Hafiz
...and AJ&K for further propagation and raising of plantations. Pollarding at 30 to 70 height cm from ground level was suitable in mulberry plantations on agriculture flat land and 70 cm to 170 cm height in irrigated forest plantations. The best planting time for mulberry was 15th December to 31st January in Punjab and N.W.F.P....
Muhammad Farooq, Pazir Gul Khan and F. W. Khan
...well to justify their propagation as a cattle feed on pilot scale....
Mahmood Iqbal Sheikh
...se species should be propagated on a large scale for bringing back the desert vegetation for fuel, fodder and food....
K.M. Siddiqui, M.I. Sheikh and S. Rehman
...species can be easily propagated asexually and the application of hormone had no significant effect on rooting. The narrow and broad-sense heritability was low emphasizing the importance of uniform site conditions and inclusion of a large number of progenies and clones in test plantations. These investigations suggest that a suitable breeding strategy would further bring about genetic improvement in Populus ciliata....
M. Saleem Khattak, Hafiz Inayatullah and Sharafat Khan
...tiation and economic propagation of olive, Olea europea L. cultivar Leceino cuttings. Three growth regulators namely, Indole 3-acetic acid, Indole 3-butyric acid and 1-Naphthalene acetic acid each with 3000, 6000 and 9000 PPM in 50%alcohol were used as Quick Dip method on the semi hardwood cuttings of the aforesaid species. Better routings (22.25%) were produced with Indole 3-butyric acid at 6000PPM as compared to I.A.A. and NAA. The non...
Mahmood Iqbal Sheikh
...erial for large scale propagation...
Adil Al-Kinany
...ng in GDR, vegetative propagation of Populus alba, Populus tremula, Picea abies and Jniperus communis by cuttings using different concentrations of IBA and NAA singly as well as in mixture was studied from February, 1979 to June, 1979 under glass house conditions at the Department of Biology, Dresden Technical University, Dresden. The choice of the species for this work was predominantly guided by their close similarity in silvicultural b...
Mahmood Iqbal Sheikh
...due to evaporation or seepage, the plants are supplied with a quantity just sufficient for their survival and growth....
M. Bashir Chaudhry (Dr.), M. Alim Mian and M. Rafiq
...in water table due to seepage from the canal system. The recent high water table conditions have, however produced o salt puff on these soils. The saline soils that have been grouped in five main categories occupy 14.3 million acres in the Indus plains but only 7.8 million acres in the canal command areas. The saline cultivated soils of primary practical importance are of two types: (1) soils with topsoil salinity sodicity occurring in cultivated fields, (2) p...
Moinuddin Ahmad, Asrar Hussain and Tariq Hussain
...Bor were vegetatively propagated in the grass nursery of Botany Department, University of Karachi. Various stage of their life cycle were studied under field and laboratory conditions inflorescence development period, an thesis time, number of sterile and normal florets per inflorescence were determined, and pollen grain fertility, pollen grain germination and viability and seed germination tests were carried out. Mean size, shape and weight of seed an...
Mahmood Iqbal Sheikh
...ferent sources and to propagate the same by growing at close (3x3 ft) and wide spacing (3x3 ft) and planting out the latter when one year old. The results at the nursery stage as well those of field planting are quite encouraging. ...

Sundus Oun Ali Al-Zaini1, Mohanad A. Al-Bayati3*, Khazaal Abbas Khazaal2, Salma Talib Salih1

...m common carp fish to propagate the Koi herpes virus. The objective is to develop a vaccine with a dose containing the antigenic standard of 1×102.5 TCID50/dose, aiming to achieve a heightened level of immunity compared to the outcomes observed in the initial phase. Additionally, we aim to combine the two vaccine agents, namely the virus and bacterial components, into a single product. Conclusion the study successfully developed and tested two liposomal ...

Abdul Basit1 and 2Sana Ullah*

...rtilizer application, propagation method, propagation time, transplanting time,
field situation and finally problems of the nurseries were studied. All the agricultural and
horticultural practices were not fully applied and practiced in proper recommended way. Out
of the total studied parameters, irrigation system that was sprinkler irrigation, was
impressive and appreciable. P...

Saima Naz1*, Moazama Batool2*, Qurat Ul Ain2, Ahmad Manan Mustafa Chatha3, Sheeza Bano2, Sadia Nazir4, Ghulam Abbas5 and Unab Zahra1

...on fish therefore its seepage into aquatic bodies should be carefully monitored.

...

Emad M. Gad1, Haidy G. Abdel-Rahman2, Mohy Eldin Abd-El-Fattah1, Merna M. Kamal1, Ahmed Shaker Eltahan3, Amina A. Dessouki3*

...ooked at the impact of dapagliflozin, mulberry fruit and leaves extracts and their combination on the kidney of diabetic rats. To induce diabetic nephropathy, experimental rats were supplied with 10% fructose (Fr) in drinking water for the first two weeks. Each Fr-fed animal received an intraperitoneal injection of a low single dose of STZ (40 mg/kg) after being fasted for the whole night. Sixty albino rats were separated into six equivalent groups. Group I co...

Amal Mahmood Alwan1*, Thekra Atta Ibrahim1, Ghalib Idrees Atiya Ali2 

...estigate the effect of empagliflozin in Albino rat animal model. The drug is previously known to impact the histological structure of the kidneys of male rats, however, mechanistic insights remained unknown. To investigate generic impact of the drug in animals, 15 adult male rats were randomly divided into two groups. The control group consisted of 5 rats, while the treatment group was divided into two subgroups, each consisting of 5 rats. Diabetes condition w...

Mujahid Ali1*, Malik Muhammad Akram2, Emily Silverman3, Asif Iqbal1, Muhammad Mohsan1, Haseeb Ahsan4

... sugarcane, and rice. Seepage from rivers/canals, mismanagement, unequal distribution, pollution, and salinization are major contributing factors to the water crisis. Climatic conditions, global warming, and lack of rainfall are worsening agricultural losses in this arid environment. More awareness is needed in the farming community to focus on water-saving strategies, water budgeting, and using monitoring software to evaluate areas to conserve water. Governme...

Dheyaa Ali Aljuhaishi*, Firas Hussain Kadim Albawi 

...t invade bacteria and propagate inside them, leading to the lysis of the bacterial cells. The aim of this study was to investigate the effect of adding BP to the broiler’s diet and its effect on body performance, bacterial population of the gut and immune responses against infectious bronchitis. For this purpose, a total of 250 one-day-old Ross308 broilers were randomly allotted five treatments with two replicate pens of 25 birds per pen. Different treat...

Wan Nurfarzana Wan Mohamad Zani1, Norrizah Jaafar Sidik1*, Asmah Awal2, Nurul Izzati Osman3, Lyena Watty Zuraine Ahmad1 and Mohd Khairi Nordin4

...emand for large-scale propagation and sustained supply. Traditional propagation methods are inconvenient and time-intensive. As an alternative, micropropagation techniques are opted to overcome these challenges. This research aimed to develop a micropropagation protocol for expanding D. asper bamboo through the utilization of various pro

Wen Xiong1, Yifan Huang1, Yue Liu1, Shuyin Li2* and Baoqiang Wang3*

...tect area, artificial propagation and artificial releasing and fishing ban, were implemented in Honghu Lake. We hope that this study should provide more management recommendations that will be useful for freshwater fish biodiversity conservation and fishery management in the Yangtze River.

...

Bassant Mahmoud Emam1, Jehan Abd Elrazek Hassanen1, Abeer Gaffer Ali Hassan3, Azza Mahmoud Abdullah1, Hadeer Said Aboelnaga2, Amina Ali Dessouki2*

...maceutical sector like Dapagliflozin (DAPA). However, medicinal plant remedies, e.g., Fenugreek (Trigonella foenum-graecum), are efficiently utilized for DM treatment with low side effects. Accordingly, the study aimed to compare the effects of DAPA and Fenugreek seeds powder (FSP) or extract (FSE) on hyperglycemia, dyslipidemia, and hepatic alterations of streptozotocin (STZ)-provoked DM rats. Here, DM was triggered by administrating 10% fructose (Fr) for two...

Asim Karim*, Anwar Ali, Bilal Ahmed Qazi, Nowsherwan Zarif and Wadood Shah

...its produced in vitro propagation. The major hybrids include in vitro clone 112, Cotevisa 2 and Shan Tong. Compared to Southern European countries, Central European countries have slower rates of growth and lesser manufacturing capacities. Paulownia breeding experiments are new and often replicate cultivation of Asian or Southern European hybrids. Agronomic techniques are, nevertheless, being produced, and studies are being conducted on responses to local vari...

Athar Mustafa Laghari1*, Naeem Tariq Narejo2, Muhammad Hanif Chandio1, Faheem Saddar3, Muhammad Yunus Laghari4, Munawar Lal1, Urooj Imtiaz4, Majida Parveen Narejo5, Hameeda Lashari4, Ghulam Abbas5 and Shahnaz Rashid5

...ms of fish diversity, propagation and growth. Shannon- Weiner index (H) was used to compute richness and abundance from four locations. Highest richness and abundance were noted at site I richness (32%) abundance 429 and diversity index (DI) 0.927 correspondingly while lowest detected at site IV richness, 20% abundance 121 and diversity index (DI) was 0.87 accordingly. Three kinds of native carps like Labeo rohita (11.25%), Cirrhinus mrigala (10.88%), Cirrhinu...

Sarhad Journal of Agriculture

December

Pakistan J. Zool., Vol. 56, Iss. 6, pp. 2501-3000

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