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Science, Religion and Culture
...or armies of atheist evangelists in the art of demolishing faith, which Boghossian claims is “pretending to know things one doesn’t know.”

...

Mark Zelcer

... were geared toward a largely bilingual Jewish audience that had strong cultural ties to Yiddish (the language of Eu- ropean Jewry) and also to science. But what does it mean for an ethnicity to have strong cultural ties to science and how did it get that way in this case? Put another way, why put all this effort into producing Yiddish texts for an audience, part of which could get by in English and part of which was not particu- larly well educated enough to ...

Bruce L. Gordon

...estations of broader evangelical culture. It continues to be the most common view of the relationship between science and Scripture held in the evangelical community and, unfortunately but understandably, the view of science most non-Christians associate with evangelicalism. For scientifically literate non-Christians, it presents an obstacle to Christian faith, and for young Christians who...

Mohammad Fayaz, Abbas Ullah Jan, Dawood Jan

...LI) functional form of Engel equation. The quantity and expenditure elasticities remained less than unity with reasonable differ- ence in magnitude across urban and rural households. Quantity and expenditure elasticities of all the vegetables were higher for rural households than urban. Quality elasticities for the products under study were calculated as the difference between the expenditure and the corresponding quantity elasticities. Overall, the quality el...

Marcus Mann

...California – Los Angeles, woke up with an itch. He had been shaken to his core by the events of the previous day and found himself drawn to his computer, relating his thoughts on the 9/11 terrorist attacks’ implications to a word processor. What started as an itch evolved into his first book, The End of Faith: a scathing indictment of religion, which in his opinion, was an impetus just as effective and real as the jet engines that propelled the ter...

Ummara Waheed Khan, Raza Ahmed, Irum Shahzadi and Mohmmad Maroof Shah

Email | [email protected]

... success depends largely upon the average effect of several important factors including explant tissue, culture medium, plant genotypes, and their interactions, the detail of which is not fully explored. Current article described this issue in detail. The information synthesized would be important to select and manipulate appropriate factor(s) for successful wheat tissue culture and regeneration that in turn
would help its improvemen...

 Mohammad Fayaz, Abbas-ullah Jan, Dawood Jan, Ghaffar Ali and Yousaf Hayat

...verse specification of Engel curve for cereals’ quantity and quality response to households’ income in Pakistan. The elasticities of interest (quantity, expenditure and quality) were inelastic with reasonable difference in magnitude across urban and rural households. Overall, the quality elasticity turned out to be positive with an estimated value of 0.0061 and 0.2375 for wheat & wheat flour and rice & rice flour, respectively. The estimati...

Robina Karim and Dawood Jan

...ade is expected to be largely better off once WTO’s regime is fully implemented in Pakistan and rest of the world”. The statistical results show that, the coefficient of variation of Pakistan exports trade estimates at 0.1657 during 1985-90 and 0.0936 during 1990-95 pre-WTO periods, which with the exception of sub-period 2000-05 decreased throughout post-WTO period, which suggests Pakistan exports got smoothened after WTO’s implementation in ...

Rashid Ahmed Khan* and Muhammed Naveed

...n. Its management has largely relied upon the indiscriminate and injudicious use of conventional insecticides resulting in problem of insecticide resistance, environmental pollution, fruit contamination and health hazards. The major goal of the present study was to evaluate the effectiveness of a bio-insecticide, emamectin benzoate with other conventionally used insecticides against B. zonata. The results revealed the high toxicity of emamectin benzoate with L...
Sameera Akhtar1*, Muhammad Akram Muneer1, Khushi Muhammad1, Muhammad Yasin Tipu1, Muhammad Anees2, Imran Rashid1, Raza-ur-Rehman3 and Irshad Hussain1
...r characterization is largely inadequate and requires continuous evaluation. In this study, sequencing and molecular analysis of fusion (F) and hemagglutinin (HN) genes of NDV strain isolated from an outbreak in a peacock flock was undertaken. Proteolytic cleavage site of F0 showed a cleavage motif (112RRQKR↓F117), representative of a velogenic serotype. Moreover, based on F and HN gene nucleotide sequence analysis, the is...
K.N. ArulJothi1, M. Abinaya1, B. Suruthi Abirami1, Melvin George2, S. Elangovan3 and A. Devi1*
...y PCR-RFLP combined with gel electrophoresis. The genotype distribution in the population was found to be consistent with Hardy Weinberg equilibrium. Through logistic regression analysis, it was observed that, in Tamil population the CC and CT genotype carriers have higher odds of developing myocardial infarction (OR – 1.64 and  2.04 respectively) and for developing hypercholesterolemia (OR – 2.02 and 2.13 respectively). In the total populatio...

Muhammad Khurshid1,2*, Muhammad Nafees1, Mehmet Somuncu2

...ns of HKH region have largely transformed this subsistence livestock production in to commercial agricultural system and shaped an uncertain environment that is not suitable for both landless nomadic pastoralism and ecological sustainability. This study reflects that how this agriculture encroachment may have contributed to the pastoral resources degradation and subsequent landless pastoralist’s marginalization in uplands of Naran. Extensive field survey...

Wazha Mugabe1,2, Lawrence Akanyang1, Mackenzie Nsinamwa1, Batanani Moatswi1, Naledi Matthews1, Kealeboga Dipheko1, Imtiaz Ahmed Ujjan3 and Assar Ali Shah2*

...nding the dynamics on rangeland response to grazing could prove worthy in finding an equilibrium point for optimizing animal productivity, with limited range degradation. Therefore, the current study was aimed at determining and comparing fodder tree species composition along grazing gradient in fenced and unfenced grazing area in the Gaborone North Region. For each study area, two parallel transects measuring 1000 m radiating from the water points (borehole) ...

M. S. A. Mamun and M. Ahmed

Integrated pest management in tea: prospects and future strategies in Bangladesh
...ck of these pests and largely responsible for the declining productivity of tea. Extensive use of chemical pesticides began only a few decades ago with tremendous immediate economic gains but its abuses were not foreseen or ignored. As a consequence there arose the development of resistance to pesticides, pest resurgence and undesirable pesticide residue in the made tea as the major problems. Current trends in eco-friendly insect pest management practices emph...

Anjan Bhattacharyya, Suhrid Ranjan Barik & Pritam Ganguly

New pesticide molecules, formulation technology and uses: Present status and future challenges
... like water emulsifiable gel, floating granules, drift less dust, macro and micro encapsulated suspension, hollow fibers, monolithic matrix, laminated structures etc can avoid these problems. The prime motto for these developments is to give protection to the crops along with safety to the natural enemies of different pests as a whole safety to environment.
 

 

...
Bushra Niaz1,*, Tahir Zahoor1, Muhammad Atif Randhawa1 and Amer Jamil2
... sulphate-polyacrylamide gel electrophoresis (SDS-PAGE). Disc diffusion method was applied for the evaluation of antibacterial activity of lactoferrin. Minimum inhibitory concentration (MIC) of lactoferrin was determined by 96-well plate method using ELISA reader. Results depicted that CM-Sephadex C-25 was a good cation exchanger for recovery of lactoferrin. Camel raw milk produced 2.4 mg/mL of lactoferrin. The lactoferrin isolated from camel milk exhibited pr...

Hassan Hashim Ghalib, Syed Attaullah Shah*, Abbas Ullan Jan and Ghaffar Ali 

...turn per acre could be hugely affected by temperature increase due to climate change. This change in temperature could positively affect wheat growers’ net-return in the Northern zone. Development of new seed varieties with increased heat tolerance and provision of timely and sufficient water for irrigation would be helpful in reducing future potential losses to wheat growers.  

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Abbas Ullah Jan1, Muhammad Fayaz1*, Yousaf Hayat2, Dawood Jan3 and Ghaffar Ali3 

...representing nonlinear Engel curve relationship proved to be a valid estimation technique for fruit consumption in Pakistan. The results show that quantity elasticity with respect to income is constant for dates, grapes, guava and mango and tends to increase in all fruits, apple, banana and citrus with increase in household income for urban households. For rural households with increase in household income, quantity elasticity of all the fruits tends increase ...
Amtul Jamil Sami1,*, Madeeha Khalid1, Rehman Shehzad1, Sana Mughal1 and A.R. Shakoori2 
...lactogen was purified by gel filtration chromatography using Sephadex-G100 and ion exchange chromatography on DEAE- Sepharose, employing a linear salt gradient. SDS-PAGE was used to monitor the purification steps, and protein band was located by immunoblotting technique. Bubaline placental lactogen was purified to homogeneity and showed a molecular weight of about 30 kD. Purified placental lactogen was administered to male albino mice for up to eight weeks for...
Mumtaz Akhtar1, Shabana Naz2,*, Muhamamad Iqbal3, Waqar Azeem4 and Mirza Azhar Beg5
...s V animals comprised largely of heavy animals. In the winter sample, animals of Age Class III, IV and V were dominant, while in the spring sample, animals of Age Class IV and V were predominant. Animals of Age Class I were represented for the first time in the summer sample. It was also observed that the seasons influenced the body weight of the gerbille at a statistically significant level (P < 0.01). Sex did not seem to be an important factor in this res...
Hafiz Muhammad Ishaq1, Muhammad Shahzad2, Xiaokang Wu3, Chaofeng Ma4 and Jiru Xu1,*
...of most dominant excised gel bands was done. Real-time PCR was performed to evaluate the copy numbers of dominant bacteria of gut microbiota. The results indicated that a significant diversity difference between asthma and healthy groups (*P< 0.05), was shown in DGGE profile. Similarity index was found to be lower in inter-group than intra-group that indicated a change in composition of gut flora in asthma patients. Sequencing results also indicated ...
Faiza Hassan1, Mubshara Saadia1,*, Muhammad Sher1, Mian Anjum Murtaza2, Muhammad Arshad3,Asam Riaz4 and Mahmood Ahmad Khan5
...red to black cumin (Nigella sativa oil; NSO) and insulin. Alloxan induced diabetic rats were post treated with plant extracts (150 mg/kg) and serum samples were collected from overnight fasted diabetic rats on day 15 to observe thepercent changes in blood glucose levels and lipid profile. Garlic extracts have shown the significant (p<0.001) hypoglycemic activity with no considerable effect on mean body weight of rats (ns, p>0.05) as compared to di...

Wang Jian1, Abdur Rehman2*, Noor Khan3, Wang Zhengjia4, Ji Peng-fei5, Xie Cong6, Muhammad Niamatullah Khan Babar7 and Raheel Saqib8 

...asing trend except the Engel coefficient shows the declining trend from 39.508% to 29.355%.The study of regression analysis, MPC (marginal propensity to consume) and APC (Average propensity to consume) clearly shows that in the study area with the rise of rural households income, their consumption demand also increases. The relative co relational analysis shows, that the food consumption ranks first, household consumption has second, clothing consumption third...

Sidra Majeed, Shahid Ahmad*, Asif Ali Bhatti** and Ghazanfar Abbas***

...that the increase was largely in the cost of tillage and harvesting due to increase in the price of diesel fuel and renting cost of farm machinery. The average net return from barani wheat using chemical fertilizer is Rs. 1.22 million, on an average the benefit and cost ratio was 1.28, which means that 28 % of the total cost is realized back in the form of net returns. Out of ten years, the barani wheat production using chemical fertilizer was profitable in si...

 Sarfraz Ahmad, Shaista Koukab, Nayyar Razzaq, Muhammad Islam, Aneel Rose*, and Muhammad Aslam**

...lly in many protected rangelands areas. However, its population is declining due to over-exploitation and un-controlled grazing. Traditionally these species are used for the treatment of fever and pain. Annual or German chamomile (Matricaria recutita L.), and perennial chamomile (Chamaemelum nobile) are the most important species all over the world due to multipurpose uses. Field experiments were conducted at Arid Zone Research Centre, Quetta to evaluate the g...
Xia-Lin Zheng1, Pan Wang2, Jun-Yan Liu1, Yu-Jing Zhang1, Jun Li1 and Wen Lu1,*

 Rabeea Tariq*, Khalid Mahmood Qureshi*, Imran Hassan*, Muhammad Rasheed* and Usman Shaukat Qureshi*

EFFECT OF PLANTING DENSITY AND GROWING MEDIA ON GROWTH AND YIELD OF STRAWBERRY
...cause plant growth is largely depended on the physiochemical properties of the growing media used. Winter strawberry production in a greenhouse using high plant densities and various media may be a viable alternative to openfield production system. Planting density can be increased thrice by using different production systems. Studies were conducted to see the impact of different planting densities and media on growth and yield of strawberry. The treatments we...

 Quratulain*, Muhammad Aslam**, Muhammad Khalid Rafique*, Mian Atiq Ahmad* and Rashid Mahmood***

POPULATION DYNAMICS OF ROSE APHID MACROSIPHUM ROSAE L. ON DIFFERENT CULTIVARS OF ROSA INDICA L. IN PAKISTAN
...hid nymph, winged and wingeless adults on leaves, buds and flowers were 11.11, 4.97 and 10.13, respectively observed on Perfecta variety

...

 Hafiz Saqib Habib*, Arshad Mahmood Malik*, Abid Ali** and Muhammad Aamir Khan***

SOCIOECONOMIC DETERMINANTS OF RURAL HOUSEHOLD FOOD EXPENDITURES IN RAWALPINDI
...rital status variable. Engels law analysis regarding necessities such as food items was confirmed by the food expenditures value of 0.20. The study provides important food policy tools reducingnegative impacts ofhigher food expenditures on ruralhouseholds.

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  Mohammad Umar Farooq* , Sarfraz Ahmad ** , Mohammad Islam and Imtiaz Ahmad Qamar ***

ASSESSING THE STATUS OF CARBON POOL IN TWO RANGELAND GRASSES ( ) IN POTHWAR, REGION, PAKISTAN
... was conducted in two rangeland grasses and in field area of Rangeland Research Institute (RRI), National Agricultural Research Centre, (NARC), Islamabad. Carbon is considered as the most important component of green house gases. Carbon sequestration during the photosynthesis process via plant biomass is the extent of this atmospheric gas. Data for above and below phytomass for two rangela...

 Arshia Naeem*, Maria Anjum*, Mariam Rehman*, Zahid Mahmood** and Muhammad Asif Kamran**

AN INTEGRATED INFORMATION SYSTEM TO FACILITATE FARMERS IN WHEAT, SUGARCANE AND OTHER CROP DISEASES IDENTIFICATION
... as Pakistan, economy largely depends on agriculture. Therefore, the need to move from conventional ways of farming to easy, effective and efficient smart solutions are required to minimize yield losses in crop plants. An integrated application to facilitate farmers to communicate their crop related issues directly to agriculture scientists is proposed. This 'AXPERT Platform' consists of web application that provides user centered interface to farmers and a de...
David Beckwith Greene
...iv>According to Thomas Nagel, neo-Darwinians do not recognize the radical discontinuity between subjective experience and materially conceived reality. He calls for a new understanding of preconscious matter such that genuine continuity between it and subjectivity may become intelligible. According to Monroe Beardsley, music presents modes of continuation. An analysis of modes of continuation in Haydn’s “The Creation” shows that the co...
Abdul Nabi Jatt1,*, Sarfraz Ali Tunio1, Shaista Bano Memon1, Abdul Sattar Qureshi2 and Muhammad Aqeel Bhutto
...e bacterial strain Shigella dysenteriae IM using API-ZYM test system. Out of nineteen different enzymes, S. dysenteriae IM strain was capable to poroduce nine enzymes, i.e., alkaline-phosphatase, acidic-phosphatase, lipase, valine arylamidase, cystine arylamidase, napthol-AS-BI-phosphohydrolase, β-galactosidase, leucine arylamidase, and trypsin. While, esterase, esterase lipase, α-chymotrypsine, α-galactosidase, β-glu...

Baharullah Khattak1*, Saifullah2, Shaukat Hussain2, Musharraf Ahmad2, Asad Ali2, Mohammad Junaid2, Ijaz Ahmad Khan3, Taj Ali Khan1 and Mubbashir Hussain1 

...resulted in 113 bands on gel for the 15 isolates of T. harzianum. The analysis of the bi-variate data, using Unweighted Paired Groups Method Averages, divided the indigenous isolates of Trichoderma, into four well-defined clusters. These results clearly revealed that there were considerable genetic differences among the Trichoderma isolates. While, there was no correlation, found between RAPD clusters and aggressive groups (AGs). Isolates belonging to differen...
Nina Bao1,*, Yongguang Yin2 and Panpan Wang3
...entified by using silica gel column and TLC, respectively. IR analysis showed that the extraction has hydroxyl and amide groups, which was proved to be cerebroside. Meanwhile, bioactivity showed that the extraction has antibacterial activity, and the purified one is better than the crude one.
...
Changqing Zhao*, Zhuochi Chen and Yubin Li
...lus bulgaricus and Angel Yeast was used to produce fermented pork jerky, and a series of indexes were measured. The results demonstrated that the pH of the fermented jerky was lower than the non-fermented jerky; the true protein content of the fermented jerky was lower than that of the non-fermented jerky; the free amino acids content of the fermented jerky was higher than the non-fermented jerky; the acid protease activity was slightly higher than the neu...
Jia Chen*, Yuanxi Deng and Hui Xu
...yzed by ion exchange and gel filtration chromatography, and the MALDI-TOF/TOF-MS search on the Internet. The results indicate that the protein was diamine- N-acetyltransferase.
...
Xi-wen Chen1,2, Qian Wang1,3, Miao Yin1, Zhong-hui Pu1,2, Ai-wei Guo3, Lian Li 1,3, Wen-tao Luo1,3 and Xiong-qing Wang1,*
...ualized using 1% agarose gel electrophoresis or color change after the addition of the SYBR Green I dye. The RT-LAMP method was approximately 100-fold more sensitive than RT-PCR for PRRSV detection. The assay was also specific for PRRSV and did not cross react with CSFV, PCV-2, PPV or PRV. In clinical samples (n=5) RT-PCR and RT-LAMP both identified the same 2 PRRSV infected samples. Thus, the novel RT-LAMP assay described here is a rapid, simple, sensitive, s...
Yang Yang1,2, Yun Liu1, Mingxu Zhou1 and Guoqiang Zhu1,*
...>Escherichia coli flagellin expression, E. coli F18ab strain 107/86 was modified to either express acyl-homoserine lactone (AHL) synthase (QS-I) or deleted for autoinducer 2 (AI-2) expression (QS-II). AHL expression and deletion of luxS (AI-2) both inhibited flagellin expression, as measured by motility assays, bacterial gene expression, and host responses to infection. The QS systems and fla
Guifen Liu1,2, Xiaomu Liu1,2, Khuram Shahzad3, Wei You1,2, Juan J. Loor3,* and Fachun Wan1,2,4,*
... of adipose tissue is largely influenced by the genetic background of cattle. Therefore, we analyzed the transcriptome changes within adipose tissue in beef cattle at different stages of growth. Microarray analysis of data at 12 vs 18 months, 12 vs 24 months, 12 vs 30 months, 18 vs 24 months, 18 vs 30 months and 24 vs 30 months uncovered a total of 10625 differentially expressed genes (P ≤ 0.05; no fold change). The dynamic impact approach (DIA) bioinformat...
Mumtaz Ali Khan1, Aneela Zameer Durrani1, Sher Bahadar Khan2, Shehla Gul Bokhari1, Ikramul Haq1,*, Imdad Ullah Khan3, Naimat Ullah4, Naimat Ullah Khan4, Kashif Hussain1 and Azmat Ullah Khan2
...with aluminium hydroxide gel. Safety, sterility and stability of the toxoid were ascertained. Bacterin toxoid of C. perfringens type D was procured from Veterinary Research Institute Peshawar. The immunogenicity of toxoid vaccines and bacterin toxoid vaccine were studied and compared initially in rabbits and then in goats and sheep. Experimental trials were conducted on 54 healthy animals of each species and were divided into three equal groups (n=18). ...
Nuzhat Huma1, Fozia Ghaffar1, Saima Rafiq2,*, Imran Pasha1, Aysha Sameen1, Imran Hayat2 and Imtiaz Hussain2
Kiran Aqil1, Muti-ur-Rehman Khan2, Asim Aslam2, Aqeel Javeed5, Rizwan Qayyum1, Farooq Yousaf,1 Farkhanda Yasmeen1, Muhammad Luqman Sohail3 and Sajid Umar4,*
...of six dilutions of Nigella sativa (N. sativa)alcoholic extracts against the PPRV was investigated in vitro. Vero cell lines infected with PPRV were treated with six dilutions alcoholic extracts of N. sativa (200, 100, 50, 25, 12.5 and 6.25 µg/mL). The antiviral effects were judged by 3-(4,5)-dimethylthiahiazo (-z-y1)-3,5-di-phenytetrazoliumromide colorimetric (MTT) assay. Plaque reduction assay was used to test the mode of action. O...
Yang Liu1,2,3, Baimei Liu1,2,3, Chenchen Li1,2,3 and Meiwen An1,2,3,*
...m with pressure.Chitosan-gelatin scaffolds were fabricated by vacuum freeze drying. Keratinocytes and fibroblasts were seeding on scaffolds to form systems as: keratinocytes monoculture (KM), fibroblasts monoculture (FM), co-culture of keratinocytes and fibroblasts (KM&FM). Systems was treated with 3.4 kPa pressure for 1 day, systems without pressure was considered as control groups. The mRNA expression and protein secretion of supernatant were tested us...
Şehriban Çek Yalnız 1,* and Erdal Yilmaz2
...s function in fish is largely unknown. In order to discuss the possible role of apoptosis in fish ovarian follicular atresia and postovulatory regression, the pre and postovulatory follicles (POF) of the freshwater teleost, Clarias gariepinus were observed by light microscopy. The germinal vesicle and the cytoplasmic organelles of the oocyte were disintegrated. The theca of atretic mature oocytes was hypertrophied and persisted to form the interstitial ...

Asma Sohail1*, Kashif Sarfraz Abbasi1, Maryum Arif2 and Fatima Najam1 

...l was encapsulated using gel entrapment i.e. extrusion method and indirect diffusion method using Ca-alginate. Pumpkin seed oil macrospheres showed 91.9 % encapsulation efficiency and 63.0 % loading capacity by direct extrusion method. However, indirect or diffusion method showed 43.0 % and 24.7 %. These results showed that direct method of encapsulation was suitable for oils to achieve maximum encapsulation efficiency and loading capacity at higher concentrat...

Nadia Sharif1, *Neelma Munir1 and Shagufta Naz1

MICROBIAL PRODUCTION OF ALGINATE
... properties like ease of gelation and biocompatibility promote its applications in engineering and bio medical science.

...

Muddasir Hassan Abbasi1, 2, Noor Fatima1, Syed Shahid Imran Bukhari1, Asma Rashid khan3, Nadeem Sheikh2*

Variations in proteins and transaminases following experimental induction of Bisphenol A in mice
...te
polyacrylamide gel electrophoresis (SDS-PAGE) was also run to analyze protein bands. Statistically significant
increase in total protein contents, albumin and aminotransaminases were noted in sample in comparison with control.
Comparative analysis between experimental and control samples revealed protein fractions (KDa) of 149, 132, 101,
72, 75 and (163, 114, 91, 75, 72, respectively. Conclusively, it can be stated that extensive...

Farkhanda Asad1*, Samina Qamer1, Tayyaba Ali1, Ammara Behzad1 and Tahira Yasmin2 

...ility coefficient (%) of gelatinized and non-gelatinized corn in Cirrhinusmrigala fingerlings for 90 days. Using various levels of chromium chloride hexahydrate six test dietsdesignated as; T1(G/0.0, CrCl3.6H2O mg Kg-1), T2(NG/0.0, CrCl3.6H2O mg Kg-1), T3(G/0.2, CrCl3.6H2O mg Kg-1), T4(NG/0.2, CrCl3.6H2O mgKg-1), T5 (G/0.4, CrCl3.6H2O mg Kg-1) and T6(NG/0.4, CrCl3.6H2O mg Kg-1) were prepared. Results showed highest apparent ...

Syed Amjad Kamal Jan* and Naushad Khan 

...e 5.434 Kg per acre averagely of the respondents. According to analysis Farm Size, Tractor hours, Fertilizers, Number of irrigations, Farmer’s education were found significant at 5% level while , Seed Variety, Pesticides, Farming experience, Number of labors were found non-significant at 5% level. The R-Square value was 0.92 which means that 92% variation is explained by the independent variables on the dependent variable in the model. It seems very good...
Jam Nazeer Ahmad1,2,*, Muhammad Jafir1, Muhammad Wajid Javed1, Sumaira Maqsood3 and Samina J.N. Ahmad1,2,*
...he PCR bands obtained in gel electrophoresis amplified PCR fragments from all DCB species at 710 bp. The sequencing results and phylogenetic analysis with sequences submitted at NCBI database revealed that DCB have 99-100% similarity with Oxycarenus hyalinipennis OH1 (JQ342987.1), Oxycarenus hyalinipennis OH2 (JQ342988.1) reported from USA. This is the first report of molecular identification and DNA barcoding of Oxycarenus hyalinipennis i...
Rishen Liang*, Meng Zhou, Zhenxiang Lin, Guozhang Li, Yuan Chen, Xuan Lin and Zaohe Wu
...pectively, which were largely greater than the threshold of species diagnosis divergence value of 2%. Inner individual divergence values of each species were less than 0.5%. In the molecular phylogenetic trees, the longer branch length also clearly distinguished the independent placement of the two species. These results revealed great genetic differences between P. orientalis and P. vittatus, and strongly suggested that they might be two distinc...
Saleha Gul1,2, Muhammad Khisroon2, Ajmal Khan2, Attaullah1, Saira Gul3 and Gul Nabi Khan1,4,*
... on other tissues are largely unknown. We explored the genotoxic effects of tobacco smoke on peripheral lymphocytes of active and passive smokers. Blood samples were isolated from 100 males’ including 25 active-, 25 passive- and 50 non-smokers. Alkaline comet assay was done and classes were defined on the basis of comet tail length. Significant differences were found in total comet score (TCS) among different groups (p˂0.05). The TCS value of act...

 Asad Sultan1, Rabia Ali1, Rifat Ullah Khan2,*, Sarzamin Khan1, Naila Chand1 and Ambrina Tariq3

...p;Poultry industry is largely based on cereals for energy component in poultry ration. However, certain anti-nutritional factors hamper the bioavailability of nutrients and need to be addressed to avoid compromised birds’ performance. The present study assessed the impact of phytase on enhancing the nutrient retention and phosphorus utilization in two different sorghum cultivars fed to broiler. In an open sided house, a total of 160, 14-days-old broilers...
Faiz-ul Hassan1,*, Muhammad Sajjad Khan1, Muhammad Saif-ur-Rehman1, Syed Muhammad Raihan Dilshad2 and Muhammad Amjad Ali3
... separated on an agarose gel electrophoresis and scoring of bands was performed. Phylogenetic tree was constructed by UPGMA method to determine the genetic similarity among the horse breeds. Thoroughbred and Arab horse breeds showed a higher similarity with Pakistani indigenous breeds, suggesting that these two exotic breeds had contributed significantly in the development of indigenous horse breeds of Pakistan. One set of primers OPL-7 revealed a polymorphic ...
Faiza Jabeen1,*, Bushra Muneer2 and Javed Iqbal Qazi3
...and polysaccharides like gelatin and starch. Phylogenetic analysis based on 16S rRNA gene sequences revealed that both isolates belong to the same specie Anoxybacillus rupiensis with 99% similarity under Accession number KF254911 (JF82) and KC849452 (JF83). The DNA G + C contents were 56.854% and 56.754% for A. rupiensis JF82 and A. rupiensis JF83, respectively. The isolates grew over a wide range of temperatures (40-70°C) and pH (4-9)...
Zahra Nazir1, Saba Ijaz1, Roquyya Gul2 and Mahjabeen Saleem1,*
...t with ammonium sulfate, gel filtration on Sephadex G-75 column and ion exchange on Q-Sepharose column chromatography. Polygalacturonase was recognised as a protein with 47kDa molecular weight by SDS-PAGE. The optimum pH of purified polygalacturonase activity was found to be 4.5 and stable within pH range 3.5-5.5. Temperature dependent studies revealed temperature optimum of enzyme to be 40°C and stable up to 60°C. Among substrates, polygalacturonic ac...
Iram Liaqat1,*, Nazish Mazhar Ali2, Najma Arshad3, Riffat Iqbal1 and Zain-ul-Abideen1
...e protein filament of flagellar apparatus producing significantly controlled biofilm formation which might also be helpful to control clinical infections associated with Desulfovibrio spp.
...
Mohsen M. El-Sherbiny1,2,*, Reny P. Devassy1, Erinn M. Muller3, Abdulmohsin A. Al-Sofyani1 and Ali M. Al-Aidaroos1
...rsity followed by dinoflagellates. A total of 138 phytoplankton species (80 diatoms and 57 dinoflagellates) contributed to the phytoplankton diversity during the study. Zooplankton exhibited clear diel variation in distribution with higher abundance during night. Among the thirty-zooplankton taxa observed, Copepoda formed the dominant taxa. Of the total 70 copepod species observed during the study eight species are new recor...
Jing Hu1,2, Yan Liu2,3, Gang Yu1,2, Changping Yang2,3, Binbin Shan2,3, Shengnan Liu2,4 and Dianrong Sun2,3,*
...eny in larval A. schlegelii, which provides fundamental information for future artificial breeding and taxonomy of A. schlegelii and other relative species.
...
Tian-Yi Zhao1, Zi-Qing Liu2, Bo Yang1, Qiao Gao1, Hong-Yu Zhang1, Pei-Yu He1, Ming-Hua Duan1* and Yu-Li Yan1*
...pression.
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Aqsa Javaid and Nageen Hussain*
...r analyzed on 1% agarose gel. Sequencing was done through genetic analyzer (3500 ABI). No mutation was observed in FOXP3 gene exon 1of Pakistani HIV patients.
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Attaullah Khan Pathan1, Imran Ali Rajput1*, Agha Mushtaque Ahmed2, Muhammad Waseen Kalroo1, Muhammad Munir Shahid4, Abdul Qadir Baloch3 and Faheem Ahmed Rajput2 

...Highest number of Bracon gelechiae found than other in all selected districts. Parasitoids were active during the month of November. 

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Asad Ali Khaskheli1*, Gulfam Ali Mughal1, Gul Bahar Khaskheli2, Allah Jurio Khaskheli3, Arshad Ali Khaskheli4, Abdul Samad Magsi5, Ghulam Shabir Barham2, Arab Khan Lund5 and Maqbool Ahmed Jamali4 

Abd El-Nasser Ahmed Mohammed1,2,*
... of 1.0 and 2.0% of Nigella sativa (N. sativa) oil to female mice and secondly to explore if dietary N. sativa is effective in alleviation of hypothermia and hyperglycemia due to general anesthesia. Seventy-five albino female mice (21.74 ± 0.19) were distributed into three groups; control (G1; N=25; not receive N. sativa oil) and two N. sativa oil groups supplemented with either 1.0% (G2; N=25) or 2.0% (G3; N=25) for 3...
Hafiza Mehwish Iqbal1, Shahid Yousaf1, Salman Khurshid1*, Qurrat Ul Ain Akbar1, Saqib Arif1, Nasreen Fatima2 and Ali Murad Rahoo3
...istan. Its quality is largely influenced by postharvest handling and conditions that imparts huge economic and health implications when not been addressed properly. This study has been attempted to assess the quality of citrus in terms of postharvest physiological disorders and organoleptic properties in relation to the occurrence of pathogenic diseases. Fruit samples (injured and healthy) were taken from the three different local markets of Karachi. Some of t...
Muhammad Suleman1,*, Abu ul Hassan Faiz2, Muhammad Shahbaz2 and Ayesha Riaz3
...rlenmeyer flasks using vogel’s medium at 35°C for five days. UV irradiated A. niger has significantly increased the xylanase production as compared to parent strains. Twenty minutes exposure of A. niger was considered suitable for the production of xylanase. Wheat bran concentration (3.5%) was considered best substrate for xylanase production than corn cobs and sugarcane baggasse. Maximum xylanase production was observed at 30°C wit...
Yue Ren1, Peng-Fei Liu2, Wan-Long Zhu1,*,Hao Zhang1 and Jin-Hong Cai1
...in higher altitude (Xianggelila, XGLL and Deqin, DQ) than that of lower altitude (Ailaoshan, ALS, Jianchuan, JC and Lijiang, LJ). In winter, body mass and serum leptin levels were lower, and food intake, RMR, NST, NPY and AgRP expressions were higher in XGLL and DQ. All of the results suggested that E. miletus can successfully overcome the physiological challenges of an cold temperature in winter by increasing thermogenic capacity, food intake and decre...
Syed Maaz Nadeem1*, Muti ur Rehman Khan1, Asim Aslam1, Ali Ahmad Sheikh1, Arfan Ahmad1* and Muhammad Anees2
...r characterization is largely inadequate and requires continuous evaluation. In this study, sequencing and molecular analysis of specific regions overlapping VP1-VP2 and VP2-VP3 genes of CAV strain from outbreaks in broiler flock (n=45) were undertaken in the Punjab Province of Pakistan. The phylogenetic analysis clustered study strains of CAV in group II similar to those reported previously from China. These findings are helpful for an understanding of curren...
Qaisar Jamal*, Akram Shah, Syed Basit Rasheed and Muhammad Adnan
...r end, having as long flagella as one and a half to twice the body. The nectomonads changed to leptomonads with a ratio of 5% and 44% on day 2and 3 respectively. The body to flagellum ratio decreased in leptomonads giving a wider and shorter appearance than the nectomonads. On day 4, the log phase, the metacyclic promastigotes appeared in the culture. The metacyclic to leptomonad to nectomonad ratio was 27%, 43% and 29% resp...

Asim Munawar1,2*, Farooq Ahmad1, Aqsa Arshad1, Muhammad Ishaque Mastoi3 and Chengjuan Liang4 

...aphical locations was largely unknown. To overcome this gap our research was undertaken to evaluate the resistance mechanism in lab and field collected strains of R. dominica and T. castaneum against deltamethrin and phosphine. Results indicated significant difference between lab and field collected pests strains regarding their resistance mechanisms to deltamethrin as well as phosphine treatments. Field strains collected from various geographical locations we...
Sikander Ali* and S. Wajiha Khalid
...d by SDS poly-acrylamide gel electrophoresis. 
...

Jie Yang

...yroid cancer remained largely elusive. In order to investigate the regulation of miR-16, human papillary thyroid carcinoma cells are transfected with miR-16 mimics or inhibitors. Targeting miR-16 and using dual-luciferase reporter assay, qRT-PCR and Western blot, the mRNA and protein expression were detected, respectively. Cellular apoptosis were examined by flow cytometry and Western blots was applied to assess the cleaved caspase-3, -8, -9, and cleaved ...
Saeed Fatima1, Khalid Javed Iqbal1, Usman Atique2,3*, Arshad Javid4, Noor Khan2, Sonia Iqbal2, Hamid Majeed5, Hamda Azmat2, Bakhat Yawar Ali Khan6, Irfan7, Muhammad Tausif Shahid1, Gulnaz Afzal1
...nd holidaymakers spot largely for angling and feasting. The investigated five fish species are Ctenopharyngodon idella, Oreochromis niloticus, Eutropiichthys vacha, Rita rita and Sperata sarwari. The results divulged the pattern of metals concentration in fish in the order of Cd > As > Hg. However, R. rita, O. niloticus and C. idella showed higher accumulation of the metals that clearly alluded to s...
Ke Zhang1, Shuai Liu2, Qiu Chen1, Yu Wang1, Li Liu1, Bingjie Li1, Kai Ma1, Xiaoya Wei1, Aijun Li3 and Junyan Li2*
...ns using two-dimensional gel electrophoresis (2-DE) followed by western blot analysis (WB) with artificial antiserum. A total of 105 Haemaphysalis longicornis (H. longicornis) ticks were selected to prepare protein samples. Some of them were used for 2-DE, and the others were used to immunize mice to collect tick antiserum for WB. Then, 2-DE followed by WB and MALDI-TOF was carried out to screen and identify tick antigens. We identified 19 protei...
Ambrin1*, Ghulam Dastagir1, Jehan Bakht2 and Muhammad Adil1
...Klebsiella pneumonia, Shigella flexneri and Staphylococcus aureus. Highest zone of inhibition against Klebsiella pneumonia and Pseudomonas aeruginosa was shown by ethyl acetate and ethanolic extracts of O. biennis. Lowest MIC value was noted for ethyl acetate and ethanolic extracts of O. biennis against Staphylococcus epidermidis, Staphylococcus aureus, Salmonella typhi and Shigella ...
Xiuqing Hao1, Zhe Hong2, Huili Gao1, Fumin Tian1, Hongke Zhang3, Yu Zhou3, Yuming Guo3, Guang Yang1,* and Bingyao Chen1,*
...s’ distribution largely overlapped with various vessels’ concentrated area, possibly due to abundant local fishery resources and forging behavior of seabirds. The MaxEnt model with the area under receiver operating curve (AUC) value of 0.957 for training data and 0.935 for test data showed reliable and accurate prediction. The distance to coast (64.7% contribution) and bathymetry (30.7% contribution) were the major variables. The model predicted ex...

Muhammad Irfan*, Saeed Gul*, Mohammad Younas*

SOLAR STILL PERFORMANCE FOR SEA WATER PURIFICATION: A STATISTICAL AND EXPERIMENTAL STUDY
...ficant parameter that largely affects the evaporation rate. The fresh water with 240 mg/L dissolved salt has been extracted from the model sea water having dissolved salts of 35000 mg/L. The efficiency of a single basin solar still is improved up to 80 percent. 
...

 Saman Shahid1, Shahid Ali1, Muhammad Abdullah Ahmad1, Sarfraz Munir2

RE-DESIGNING AND OPERATIONAL ANALYSES OF THE SEDIMENT TRANSPORT CONTROL IN IRRIGATION CANAL NETWORK
... for both Brownlie and Engelund Hansen predictor methods. The data related to BRBD canal, including its cross-sectional properties, hydraulics and sediment parameters were acquired from the Irrigation Department, Lahore. The paper also described the problem which exists in design and operation of the canal by comparing it with original field data. The BRBD canal achieved a regime condition in 1.5 years. The results of sediment deposition produced by Brownlie p...
Jam Nazeer Ahmad1,*, Samina J.N. Ahmad1,2,*, Mubashir Ahmad Malik1, Abid Ali1, Muhammad Ali3, Ejaz Ahmad1, Muhammad Tahir2 and Muhammad Ashraf2
...fragment on 1.5% agarose gel. DNA sequence analysis of PCR product indicated that specimens shared a 99-100% identity with Schistocerca gregaria gregaria)(Forskål, 1775). Molecular phylogenetic analysis and evolutionary divergence study also revealed the formation of same cluster in phylogenetic tree with that of specific subspecies S. g. gregaria which furtherdeviate genetically not only from closely related subspecies (S. g. flaviventr...
Zhi Yijin, Shao Mingqin* and Li Quanjiang
...ches, and are limited largely to shallow water areas. Of species pairs, the pied avocet Recurvirostra avosetta and Eurasian spoonbill Platalea leucorodia have the highest habitat niche overlap, as their diet and foraging pattern are similar; these two species reduce competition by foraging in different areas. Three small-sized shorebirds are recorded in each water depth category. Both spotted redshank Tringa erythropus and common greenshan...
Safia Arbab1, Rehana Buriro1, Shams Uddin Bhugio1, Atta Hussain Shah2,  Jamila Soomro3,Qudratullah Kalwar4, Saqib Ali Fazilani1 and Waseem Ali Vistro5*
...i>S. aureus, E. coli, Shigella and Salmonella spp respectively. However, out of 50 sample, 34 (68%) were found to have different bacterial species and 16 (32%) were recorded with pure contamination. At 30 µl of crude Aloe Vera isolated bacterial organisms did not show any susceptibility, whereas at 60 µl, organisms showed quit sensitive reactions. In contrast, ethanol extract of Aloe Vera showed better result as compared to crude Aloe Ve...

Abdul Jalal, Abdur Rab* and Naveed Ahmad

Aloe Vera Gel Coating Retains Persimmon Fruit (Diospyros kaki) Quality during Storage at Room Temperature
... durations and Aloe vera gel concentrations significantly decreased the loss of persimmon fruit quality during storage at room temperature. Storage at room temperature (26±2 °C) resulted in significant decline in fruit firmness, percent acidity, and ascorbic acid content with a concomitant increase in total soluble solids (TSS), organoleptic quality (Taste) score and decay incidence. Coating of persimmon fruits with 30% Aloe vera
Oluwatoyin Adenike Fabiyi1*, Olubumi Atolani2 and Gabriel Ademola Olatunji3
Toxicity Effect of Eucalyptus globulus on Pratylenchus spp. of Zea mays
.... Its annual yield is largely hampered by nematode infestation, especially the Pratylenchus spp. Experiments were conducted in a screenhouse and under field conditions to evaluate the toxicity of fractions and essential oil of Eucalyptus globulus (Myrtaceae) against Pratylenchus spp. infecting Zea mays. The phytochemical basis of the plant was partly established using Infrared (IR) spectroscopy, Gas Chromatography – Mass Spectrometry (GC-MS), proton and ...
Zhi Chen1, Toshifumi Minamoto2, Longshan Lin3 and Tianxiang Gao4,*
...ne fishes on the agarose gel preliminarily revealed that all three kits could successfully extract eDNA from field water samples. Digital PCR results further revealed that the average eDNA concentration of Sepiella japonica extracted by three kits were (4.62 ± 0.72) × 105, (3.83 ± 0.56) × 105, and (2.35 ± 0.27) × 105 copies/300 ml, respectively. The eDNA concentrations by AxyPrep DN...
Yahui Wang1, Ling Ren1, Yishen Xing1, Xin Hu1, 2, Qian Li1, Lingyang Xu1, Junya Li1* and Lupei Zhang1*
...s to preadipocytes is largely unknown. In this study, the Platelet-derived growth factor receptor α (PDGFRα) positive progenitor cells were isolated from the longissimus dorsi muscle (LM) of fetal bovine and induced adipogenesis. To optimize the in vitro IMF differentiation model, the effects of bone morphogenic protein 4 (BMP4) and rosiglitazone during differentiation were studied. Comparing with control group, progenitor cells treated with...
Qianbao Wang, Zhengyang Huang, Shoufeng Li, Huayun Huang, Chunmiao Li and Zhenhua Zhao*
...ernal families were averagely distributed into three groups received treatments of 135.5, 125.5 and 115.5 g/d diet respectively with ME 2770 kcal/kg and CP 15.4% from 23 wk of age. Fertility trials were conducted in another three groups of F line hens with 20 hens in each group. A significantly positive interaction between dietary quantity and semen volume was observed (p<0.05) which showed that males fed with 135.5 g/d diet produced much more semen. Except...
Sheza Shehzadi1, Mohammad Umar Farooq2*, Rukhsana Kausar1, Ijaz Ali1*, Muhammad Arshad Ullah3 and Maqbool Shahbaz4
... in the field area of Rangeland Research Institute National Agricultural Research Centre Islamabad. In this regard an experimental plot of one acre area was established in the field area of RRI, NARC, Islamabad. Grass tuft of sized 15 cm was collected from the grass nursery of range land Research Institute (RRI) and plantation was carried out in one acre in barani condition. The area was laid down and plant to plant distance was 60 cm and row to row distance 9...
Saqib Nawaz1, Sher Bahadar Khan1*, Umar Sadique1, Muhammad Israr2
Mumtaz Ali Khan3, Hameed Ullah Khan4, Muhammad Irshad5, Ihsan Ali1 and Haq Aman Ullah1
...d through Gram staining, gelatin liquefaction test (GLT) and polymerase chain reaction (PCR), respectively. Out of total, 85 (31.3%) were positive for C. perfringens type D on PCR. The results revealed 6-34% prevalence of C. perfringens type D in different regions. Similarly, significantly (P˂0.001) higher prevalence was observed in lambs (54%) as compared to sheep (23%). For gross and histopat...

Imrana Khushk1, Abdul Nabi Jatt2, Abdul Sattar Qureshi1*, Choudhary Haider Ali3, Muhammad Aqeel Bhutto1, Imran Ibrahim Khaskheli1, Sartar Iqbal Panhwer1

...ccharomyces cerevisiae Angel was used for the production of ethanol, while, Bacillus coagulans, Pediococcus sp. and Lactobacillus sp. were used for lactic production, respectively. All the microbial strains were inoculated on wheat straw hydrolysate and were monitored with regular interval until stable cell growth, glucose consumption, and, ethanol and lactic acid production. Fermentation performance of the adapted strain improved over parental strains. Ethano...

Syed Ismat Hussain1, Muhammad Ramzan Asi1, Habbib Anwar1, Faqeer Ahmad1, Ateeq ur Rehman2*, Muhammad Shahid3, Syed Atif Hasan Naqvi2*, Ummad ud Din Umar2, Muhammad Asif Zulfiqar4, Memuna Ijaz1 and Hafiz Usman Shakir1

El-Habbaa, A.S.

...gen detection using agar gel
immune-diffusion test (AGIDT) and indirect-Immunofluorescence assay (IFA) and
molecular detection of viral DNA using PCR. Positive results were showed in 36 of
samples (28/30 of sheep samples and 8/10 of goat samples)upon isolation on ECE by the
3rd passage and 22 of samples (17/28 of sheep isolates and 5/8 of goat isolates) using
AGIDT and indirect-IFA. PCR detection show...

1Mona A. El-Manzalawy, 2Abeer A. Boseila , 3Hanan M. El Zahed

... with Aluminum hydroxide gel (Alum gel) or without Alum gel, then ensure the safety and sterility of these vaccines and compare between them with the local RVF vaccine with Alum gel alone in sheep. This peppermint formulation including Alum gel adjuvant was the best of choice as it induced higher immunological enhancem...

Sahar Abd El Rahman1; Mohamed Eladl2 and Mohamed M. El-Diasty3

...wed 470 bp clear band in gel electrophoresis. The sensitivity of the utilized techniques in the identification and diagnosis of bovine ephemeral fever disease revealed that the virus isolation is a gold standard technique, as well, the immunofluorescence technique. RT-PCR proved to be a rapid, sensitive and specific tool for bovine ephemeral fever disease diagnosis.

...

Safaa M. Mohammed*, Shahira A. Abdelwahab**,Fetaih,H⃰ ⃰ ,Neveen,R⃰ ,Abdel satar ,A⃰ and Mohamed S. Elshahidy**

...s and identified by agar gel precipitation test and polymerase chain reaction .Two main types of TKPV infection were observed in examined flocks ,cutaneous and diphtheritic forms. Morbidity rate ranged from 5-15%with no mortalities .75% of examined serum reacted positive to TKPV .All TKPV isolates were amplified using specific P4b primers and visualized by gel electrophoresis at 578bp.

...

Nashwa M. Helmy1, Ahmed S. Ahmed2, and Zeinab3 Y. Mohamed

...e and identified by agar gel precipitation test (AGPT) and indirect fluorescent antibody technique (IFAT) using specific hyper immune serum against LSDV. Further identifications were carried out by polymerase chain reaction (PCR) and clinco-pathological investigation. Results: The results showed that 11/30 biopsies were positive by AGPT, 19/30 by IFAT and 30/30 by PCR. While results of sero-diagnosis showed that 45/100 from apparently healthy non vaccinated ca...
Nehafta Bibi1,2, Faiq Jan3, Munawar Saleem Ahmad4 and Haitao Wang1,5,*
...m light conditions is largely unknown. Here, we compared light intensity threshold of activity in two groups of passerines, i.e. secondary cavity and non-cavity nesting. For this purpose, different species of secondary cavity and non-cavity nesting passerines were subjected to two phases of trials: in the first trial birds were released in an experimental cage and allowed them to accommodate themselves in darkness for 10 min. In the second trial we...

Ayman S. El-Habbaa1 and Mervat E. Radwan2

... (RT-PCR) and subsequent gel purification of the amplified products of G gene of BEF virus.
Results: BEF virus was circulating in this region. Sequence analysis of G-gene of this Egyptian strain,
comparing with sequences of BEF virus circulating globally and regained from GenBank, showed
100% nucleotide homology with BEF virus from Egypt 2014 but nucleotide and deduced amino acid
substitution generates were showe...
Shourok E. Aly 1, Karim Zaki1, Soad E. Elsayed2, M. Elgamal2, Ausama A. Yousef1, Mohamed A. Shalaby1
...ng an aluminum hydroxide gel-adjuvanted inactivated Rift Valley
fever virus (RVFV) vaccine.
Objective: Herein, we aimed to enhance the immunogenicity of the conventional vaccine by
developing a smaller particle size vaccine formulation through ultrasonic dispersion of aluminum
hydroxide adjuvant.
Methods: This was achieved by ultrasonication of aluminum hydroxide gel

 Yongmin Li1,2, Huabin Zhang1, Xiaoyou Wu1, Dongwei Li 2, Peng Yan1 and Xiaobing Wu1*

...cophorus (R. schlegelii and R. dennysi) were clustered together with the representative of the genus Polypedates (P. megacephalus), meanwhile, the representative of the genus Buergeria (B. buergeri) occupied the basal position in the clade of Rhacophoridae.
...

Syed Ismat Hussain1, Khalid Mehmood2, Mudassar Khaliq3, Habib Anwar1, Syed Muhammad Zaka4, Ateeq ur Rehman5*, Muhammad Shahid6, Syed Atif Hasan Naqvi5*, Ummad ud Din Umar5 and Muhammad Asif Zulfiqar7

Iram Liaqat1,*, Safdar Ali Mirza2, Sumera Sajjad3, Shaukat Ali1,*, Muhammad Faiz Qamar4 and Ikram Ul Haq5
...formation mediated by flagellum-mediated motility. Type III protein secretion systems of several gram-negative bacterial pathogens use flagella to invade foreign surfaces, host tissues and substrates. Flagellar biosynthesis and function in Salmonella typhimurium isregulated by >50 genes. Bioinformatics analysis of flagellar assembly in S. ty...
Ayu Suci Wulandari1, Siwi Indarti1,2*, Muhannad Illayan Massadeh3 and Nguyen Van Minh4
...our locations: Brebes, Magelang, Tegal and Temanggung, Central Java, Indonesia. Samples of soil, roots and tubers were collected. Soil abiotic factors are taken measurements such as pH, temperature, and organic matter. The altitudes are measured using the Geograpichal Positioning System (GPS). The results showed that there were five parasitic nematodes genera in garlic, that are Ditylenchus sp., Helicotylenchus sp., Hoplolaimus sp., Rot...

Hafiz Muhammad Tahir*, Iram Liaqat*, Junaid Nadeem, Hammad Aamir and Shaukat Ali

... but the results have largely been inconclusive. The studies either failed to consider the inhibitory effects of the processing chemicals or did not investigate whether the inhibitory potential is an intrinsic property of silk or is due to the aggregate gland secretion coating its surface, hence a borrowed property. Our study used direction application and disk diffusion method to investigate the antibacterial potential of Neoscona mukherji and N. theisi egg a...
Gong Xue-na1, Jia Ting2, Zhang Di2 and Zhu Wanlong1*
... Jianchuan (JC) and Xianggelila (XGLL) were fed a high-fat (HF) diet for 4 weeks and returned to a low-fat (LF) diet for another 4 weeks, body mass, food intake, resting metabolic rate (RMR), activity behavior, serum leptin levels, hypothalamic neuropeptide expressions and body compositions were measured. The results showed that regions and HF diet affected body mass, food intake and RMR significantly, HF diet increased body mass in E. miletus, while re...

Nazeer Ahmed Lashari1*, Saghir Ahmed Sheikh2, Aijaz Hussain Soomro2 and ShamsuddinTunio3

...ages after 3 months. The gel consistency (mm) was medium and soft with the gel distance values of 92.00 in BS-385 when packed in cotton bag for 9 months however, it was 48.00 after 3 months when packed in nylon bag. The statistical analysis showed that there was substantial variance between the varieties. Culinary superiority parameter such as WAR was ranged between 2.3 (DR-82 and IR-6). Volume expansion ratio was 0.8 in DR-...
Congfen Zhang1,Yingxiao Liu2, Linfeng He2, Fengjun Shi2, Weitang Yao2 and Xuegang Luo2,*
...on most organisms are largely remained unknown especially in insects. Antheraea pernyi pupae were injected with uranyl acetate, which concentrations were 6.25mM, 12.5mM, 25mM. Changes in malondialdehyde (MDA) and reduced glutathione (GSH) and activities of superoxide dismutase (SOD), and catalase (CAT) as well as their corresponding mRNA levels in the fat bodies of the pupae were evaluated. As a result, we found that the contents of MDA were increa...

Mst. Deloara Begum1, Md. Muniruzzaman1, Md. Salauddin2* and Md. Mostafizer Rahman1

...nella spp.12.5% (21), Shigella spp. 8.93% (15) and Klebsiella spp. 2.97% (5). In cooked fish 9 isolates were identified and species were Escherichia coli 66.66% (6) and Shigella spp. 33.34% (3). Total viable count varies from 1.28×107CFU/g to 3.74×109CFU/g. The highest concentration was found in Loitta fish and lowest in Ruhi from dried fish. Most species of bacteria isolated were resistant to amoxicillin, penici...
Muhammad Awais1,2, Waqas Ahmad3, Akhtar Rasool Asif4, Sayyed Aun Muhammad1, Iahtasham Khan1*, Muhammad Amjad Ali5, Muhammad Qaiser Riaz1 and Muhammad Saqib2 
... normal saline, 0.05% PI gel, certified Acacia honey and H-PImxr (an equal volume mixture of Acacia honey + Pyodine gel) respectively. Significant differences were found among four treatments with respect to contraction, granulation tissue, tensile strength, healing time and hydroxyproline contents measurement in both experiments. Honey and povidone mixture treated animals showed significantly improved wound contracti...

Muhammad Usman Asif*, Raza Muhammad, Waseem Akbar, Mubasshir Sohail, Muhammad Awais and Javed Asgher Tariq

Sadaf Ashraf1, Masood Ahmed Siddiqui1*, Kanwal Nisa1, Samar Ali1 and Naeem Rashid2
...sing cation exchange and gel filtration column chromatographies. Purified Pcal_0222 exhibited optimal α-amylase activity at 85°C and pH 5.5. The activity was not dependent on any metal ion. The hyperthermophilic nature and metal ion independence make it a suitable candidate for both basic as well as applied research.
...
Syed Shahid Imran Bukhari1*, Nusrat Jahan2, Muhammad Khalil Ahmed Khan3, Mariam Zaheer1, Sabir Javed1
...ts of different doses of gel extract in diabetic induced albino mice. Male albino mice, weighing (25±5g) were divided into three groups; Group 1: normal (non diabetic nontreated), Group 2: diabetic non treated (DNT), Group 3: diabetic treated (DT) mice. Group 3 was treated with three doses of A. barbadensis (200 mg/kg, 300 mg/kg and 400 mg/kg) per day for 14 days were assigned as DT1, DT2 and DT3 respectively. Aloe vera given orally produced significant...
Yang Yang1,2, Ji Shao1,2, Mingxu Zhou3,4, Qiangde Duan1,2, Xinyi Zhang1,2 and Guoqiang Zhu1,2*

...ors such as fimbriae, flagella, enterotoxin, adhesin and hemolysin under the influence of the AHLs. The results showed that under the influence of quorum sensing AHLs, the biofilm formation ability and adherence ability of ETEC were significantly reduced, and the expression of fliC and other major virulence factors were regulated. AHL signaling molecules can regulate virulence related characteristics of F4ac+ ETEC.
...

Ho Shi Hui1, Eng-Keng Seow2, Nurul Huda1* 

... test score of egg white gel. However, texture profile analysis showed that the hardness, springiness, gumminess and chewiness of egg white gel had decreased after ultrasound treatment. Ultrasound-treated duck egg white for 40 minutes showed a significantly (p<0.05) higher foaming capacity and improved folding test score. Result showed that the increase of ultrasound treatment time may have conferred detrimental effects o...
Warkoyo Warkoyo1*, Devi Dwi Siskawardani1, Anggit Ayu Pradana Siwi1, Moch. Wachid1, Ivar Zekker2 and Jumpen Onthong3
 
 
...sion with inconsistently gel-forming, syneresis probability of starch gel is high, limited solubility in water. Therefore, starch modification by crosslinking agent addition should be developed. The purpose of this research was to determine the effect of glycerol (C3H8O3) and Sodium Tripolyphosphate (STPP) — Na5P3O10 concentrations on the physical and me...

Shoaib Hameed1, Shakeel Ahmad1, Jaffar Ud Din2 and Muhammad Ali Nawaz3*

...their distribution is largely based on anecdotal information and expert judgments. The present study investigated the species’ current distribution in the Hindu Kush Range in Pakistan, gathering information on human-brown bear conflict along with other large carnivore species in the study area. Multiple survey techniques questionnaire surveys, sign surveys and camera trapping were used during the period 2008–2010 in five study blocks delineated on ...

Shakila Mumtaz1, Khalid Javed1, Muhammad Dawood1*, Muhammad Imran2, Asad Ali1 and Nazia Ramzan1

...k composition depends largely on the breed’s genetics and ration fed. Different proteins can be found in milk. Beta-caseins are thought to be more important because some serious health-related issues in humans have been reported with the consumption of A1 milk (mutated casein variant). This study was planned to investigate the polymorphism in the beta-casein gene (CSN2) in Sahiwal (40), American Holstein Friesian (40) and the crossbred (Sahiwal × H...

Farheen Aslam*, Hira Lodhi, Rasheeda Bashir, Faiza Saleem and Shagufta Naz

....5% E. coli and 6.25% shigella sp. Nucleotide sequencing of PCR product of Klebsella sp, Enterobacter cloacea and Citrobacter freudii showed 100% sequence homology. It is concluded that there is high prevalence of blaNDM-1 among carbapenem resistant enterobacteriaceae isolated from patient suffering from different diseases at local tertiary care hospital of Lahore.

...

Aymen Mabrouk*

...ent of volatile oil of Nigella sativa seeds, against the deterioration of blood indices by lead (Pb) has never been studied. Therefore, the present study was carried out to evaluate the possible beneficial effect of TQ against Pb-induced hematological changes. Adult male Wistar rats were treated with Pb (2000 ppm of Pb acetate in drinking water) and/or TQ (5 mg/kg/day, per os) for five weeks. Results obtained clearly showed that Pb intoxication significantly d...

Hamdy Abdala Elnagar*, Wael Mohamed Wafa, Moataz Ibrahim Badwy, Abdelaziz Mustafa Sakr 

...using SDS-polyacrylamide gel electrophoresis. Results showed the presence of the molecular weight of proteins of 71, 52 and 31 kDa in BP and SP; 63, 33, and 19 kDa-proteins in SP, and 28 - 30 kDa-proteins in BP is in association with bull fertility (high semen quality). Also, 48, 18 and 16 kDa-proteins were present in the BP and SP of low fertile bulls. Proteins with 16, 30, 34, 35, 36, 37, 63, 64, 175, and 316 kDa were observed in the BP of bull calves. Prote...

Mirtneh Akalu1,2*, Takele Abayneh2, Esayas Gelaye2, Behailu Tefera2, Teferi Degefa2, Vemulapati Bhadra Murthy1 

...l sulfate polyacrylamide gel electrophoresis (SDS-PAGE) assay of the partially purified MH-OMVs revealed approximately ten protein bands ranging from 25 to 104 kDa. Hemagglutination inhibition (HI) assay confirmed that the immune response was significantly increased in both single-dose and booster-dose immunized groups as compared to calves in the control group. Calves in booster-dose group reach its peak geometric mean (GM) log10 HI antibody titer (3.46 &plus...

Noora Hassan Hezam Al-Aqmer1*, Zain Aamir2, Muhammad Farooq Hanif3, Soumble Zulfiqar4, Sibgha Zulfiqar1, Mateen Izhar5 and Abdul Rauf Shakoori4*

...s were run on 2% agarose gel followed by visualization under UV light. Data analysis was done using IBM SPSS 24. We found that the distribution of ApaI genotypes was 28 (42.4%), 27 (40.9%), and 11 (16.7%) for the genotypes AA, Aa, and aa in responders and 22 (33.3%), 26 (39.4%), and 18 (27.3%) in non-responders. The allelic distribution was 83 (62.9%) and 49 (37.1%) for the “A” and “a” alleles in responders and 70 (53%) and 62 (47%) in ...
Mubasshir Sohail*, Qadeer Ahmed Soomro, Raza Muhammad, Muhammad Usman Asif and Imran Rauf
Yang Yang1,2, Hong Zhou3, Ji Shao1,2, Xinyi Zhang1,2, Pengpeng Xia1,2, Mingxu Zhou4,5, Qiangde Duan1,2 and Guoqiang Zhu1,2*
... in F18ab△hcp1, the flagella gene flic and type I fimbriae fimA were significantly down-regulated. In conclusion, hcp1 gene of F18 STEC is related to flagella expression, bacterial adherence, invasion, and secretion of Stx2e toxin. T6SS hcp1 is proved to be closely involved in the pathogenesis of F18 STEC.

...
Ateeq-Ur-Rauf1*,Wisal Khan1, Muhammad Salman Rafi2 and Nauman Khan3
... temperature trend. Strangely for substation Astore the precipitation trend imitates the temperature trend. It was also found that temperature is changing in three of the four seasons on an annual basis during the study period and there is a variation in the precipitation and Temperature trend patterns among the five selected sub-basins of the UIB depending upon the location of these basins. From the results, longer summer and shorter winter are expected in th...
Mudassar Jehan1, Masroor Ahmed Bajwa2, Mohammad Masood Tariq2, Asim Faraz3*, Abdul Samaad2, Jameel Ahmad1 and Yousaf Hassan Barozai2
...rs were amplified. After gel documentation, a total number of 97 alleles were recognized having 1 to 5 polymorphic forms (OARFCB193, OARJMP29, MAF33) to 4 (OARHH47, DYMS1, SRCRSP5). For total loci, number of alleles averaged 2.1162±0.3769. Shannon’s Information index (I) 0.6184±0.2447 and the effective number of alleles (Ne) averaged 1.6251±0.4604. Average observed, expected and average heterozygosities were found to be 0.5815±...

Syeda Rubab Zaidi* and Safdar Ali Mirza

...for further purification gel filtration and anion exchange methods were used along with protein estimation. The purified fraction was then subjected to enzymatic characterization to find its thermal stability, effect of pH and selected chemical compounds. Other significant experimental findings were; optimum incubation period was 7 days, inoculum size was 3 discs of 0.5 cm, CuSO4 as inducer improved production, laccase activity of crude extract i.e. 10.89U/ml ...

Oluwatoyin Adenike Fabiyi

...s fractionated on silica-gel (120-150 mesh) using glass column. The resulting fractions were investigated in screenhouse and field trials. The fractions were equated with carbofuran a synthetic nematicide. Each experimental pot containing 40 kg of pasteurized soil was inoculated with 1000 eggs of M. incognita in the screenhouse, while 2500 eggs was introduced to the base of each tomato plant on the field. Significant (p<0.05) increase was noted in the veget...

Limei Yuan1, Kong Yang1* and Dechun Jiang2*

...shan and Zhangixalus yinggelingensis were placed in the genus Rhacophorus sensu stricto and Zhangixalus, respectively, only based on morphological characters. The research on the genus Rhacophorus sensu lato faces major challenges due to their complex interspecies relationships, and the phylogenetic status of many species are still unclear, which hampered the taxonomy and protection of these species. In this study, we investigated the molecular phylogenetic st...

Xiaowei Huang1, Jinji Wang1, Zhun Yu2, Minghua Duan1* and Zhe Lin1*

..."text-align: justify;">Angelica sinensis polysaccharide (ASP) is a biomacromolecule that isolated from the roots of Angelica sinensis. This study aims to investigate its protective effect on kidney injury and its influence on BMP-7/Smads/TGF-β1 signal pathway in irradiated rats. Total 60 Sprague Dawley (SD) rats were randomly divided into 5 groups: the normal (normal saline), model (normal saline), and low, medium, high...

Dede Kardaya*, Dewi Wahyuni, Elis Dihansih 

...ecessary. Leaves of Asam gelugur (Garcinia atroviridis) contain hydroxy citric acid and other active substances that function as antioxidants, so the use of them in ration is expected to improve the meat quality of spent layer ducks. Sixty spent layer ducks were fed four rations including commercial rations (R1), nonconventional rations + 2% Asam gelugur leaf meal (AGLM) (R2), nonconventional rations +&t...

Iqra Ejaz1, Saima Chaudhry2 and Sarah Ghafoor1*

...of an oral muco-adhesive gel, having a novel combination of 1% curcumin and licorice, in an animal model of oral submucous fibrosis (OSMF), as a possible treatment modality. Animal model of OSMF was created by injecting an anti-cancer drug Bleomycin (1mg/mL) into buccal mucosa of animals daily for 8 weeks. The 24 male Wistar rats were equally divided into two groups; six rats (group 1A) were injected with normal saline (control group) while 18 rats (groups 1B,...

Ehab A. Fouad1*, Khaled A. Abd El-Razik2 , Eman H. Abdel-Rahman3

...with Protein A Sepharose gel. The separated IgG was dialyzed for 3 days against a 0.1 M NaHCO3 buffer pH 8.3 with 0.5 M sodium chloride and 0.02 percent NaN3 before being linked to CNBr-Sepharose 4B swollen beads. Bound fraction was eluted with mixture of glycine (0.1 M) and sodium chloride (0.5 M) at pH (2.3). Purified antigens and unbound were inspected of protein concentration. In the crude antigen, there were two bands with molecular weights of 73 and 64 K...

Ghulam Qadir1, Khalil Ahmed1*, Muhammad Ashfaq Anjum1, Quais Muhammad Affan2, Muhammad Zaighum Mushtaq3, Muhammad Amjad Qureshi4, Amar Iqbal Saqib1, Hafeezullah Rafa1, Abdul Wakeel1, Ghulam Shabir1, Muhammad Rizwan1, Muhammad Qaisar Nawaz1 and Muhammad Faisal Nawaz1

...veolens) and Kalwanji (nigella sativa), under dual stress of ECe (electrical conductivity of soil extract) 0.79, 6 and 8 dS m-1 and SAR (sodium adsorption ratio) 5.99, 25 and 35. Each crop was grown for four months and biomass yield data was recorded. Results of three successive seasons suggest that all the evaluated medicinal plants can grow under the medium salinity and sodicity level of (6 dS m-1 + 25 SAR). However, biomass yield decreased linearly with inc...

Sadaf Ansari1, Shahid Hussain Abro1, Abdul Jabbar Tanweer2, Abdullah Sethar3, Ghulam Abbas4, Sabahat Ansari1, Asghar Ali Kamboh1* 

...(15%, 10% and 0%) and Shigella dysenteriae (10%, 12.5% and 5%) in beef, mutton and fish respectively. While from cooked beef, mutton and fish the prevalence of E. coli (25%, 25% and 20%), S. aureus (15%, 15% and 10%), S. enteritidis and B. cereus (10%, 10% and 10%) were observed. The highest (p < 0.05) bacterial load (g­¹) was detected in raw (1.76×106) and cooked (6.1×104) beef than raw (1.55×106) and cooked (4.5×104) mutt...

Dede Kardaya*, Ayu Ratnasari, Dewi Wahyuni 

...eople in Indonesia. Asam gelugur (Garcinia atroviridis) leaves are known to reduce fat content in duck meat because it contains hydroxy citric acid. This study aimed to examine the effect of Garcinia atroviridis leaf meal (GALM) supplementation in fermented nonconventional diets on the percentage of spent layer duck carcasses. The study used a completely randomized design with three treatments, and 50 replicates. The treatments consisted of 50% rice bran and 5...

Yue Ren1, Ting Jia2, Hao Zhang1, Zhengkun Wang1 and Wanlong Zhu1*

...erent habitats remain largely unknown or are controversial. In the present study focuing on T. belangeri chinensis that from Mengla, Puer, Jingdong, Kunmimng, Chuxiong, Dali, Pianma and Lijiang. Using microsatellite approaches, we observed considerable genetic variation and high heterozygosity in T. belangeri chinensis populations in different Yunnan province, and Chuxiong population and Lijiang population had relative low genetic diversity. Moreover, skull mo...

Samantha Shiuan Erl Lee1, Nurul Huda2*, Yetti Marlida3, Eng-Keng Seow4 

... moisture, folding test, gel strength, texture profile analysis, foaming capacity and stability, and emulsification stability were compared. Ultrasound treatment had caused a significant decrease (p<0.05) in pH and expressible moisture, apart from improving the gel strength and folding test score of egg white gels. Improvement of gel strength was subs...

Muhammad Saif-ur Rehman1, Faiz-ul Hassan1* and Muhammad Sajjad Khan2

...were analyzed by agarose gel electrophoresis. Scoring of bands were performed to generate dendrogram using unweighted pair group method of arithmetic means (UPGMA). Six hundred and four amplified bands were observed from successful amplifications of 73 primers (average 8.2 bands per prime). Only seven primers amplified no product or unidentifiable (smeared) bands. A total of 68 polymorphic bands were observed, showing 11.25 % polymorphism on average across cat...

Abdurakhim E. Kuchboev1*, Mehmonjon Kh. Egamberdiyev2 

...species: Angiomphallia regeliana, Pseudonapaeus albiplicatus, P.maydanica, P. sogdiana, Psedonopaeus sp., Xeropicta candacharica, Deroceras reticulatum and Candaharia levanderi. The infection rate of Pulmonata snails by protostrongilids larvae was 28.2% and that of slugs - 6.8%. In addition, the first informs of P. maydanica, C. levanderi and D. reticulatum as natural intermediate hosts for M. capillaris are given.

Keywords | Lungworms, Protostrongylid...

Salma Bessalah1, Samira Jebahi2, Asim Faraz3*, Amel Raoufi2, Cem Tırınk4, Walid Dridi2, Mahmoud Daouad2, Hassib Keskes5, Touhami Khorchani1, Khaled Farah2, Abdul Waheed3 and Mohamed Hammadi1

...e. Results revealed that gelatin yield increased significantly (P<0.05) as the extraction temperature and time increased (19.71%) at 70°C for 12 h. EPR spectroscopy demonstrated that 10 kGy of radiation-induced the decomposition of side groups. New peak positions (Paramagnetic centers) were located at around 3800 G. Moreover, an enhancement of EPR peak (3300 and 3400 G) (g-factor = 2.0) was detected. In addition, free radicals trapped in the crystalline...

Oluwatoyin Adenike Fabiyi1*, Mariam Temitope Baker2 and Gabriel Ademola Olatunji2

...n chromatography (silica gel 100-120 mesh grade), which afforded fractions that were analysed with GCMS and FTIR for constituent identification. The result shows octanoic acid; hexanoic acid methyl ester; ethyl octanoate; 9, 12-octadecadienoic acid methyl ester; dodecanoic acid; octadecanoic acid methyl ester; decanoic acid; octadecanoic acid ethyl ester and tetradecanoic acid as the major components while the infra red spectral diagnostic signals agree with t...

Aqsa Javaid, Masoom Yasinzai* and Khunsa Saeed Kiyani

...ulfate precipitation and gel filtration chromatography using Sephadex G-50). The molecular weight of extracellular phytase from B. subtilis KT004404 estimated by SDS-PAGE was 30 kDa. The Km and Vmax of B. subtilis KT004404 phytase were 0.175 mM and 250 U/mL (phytase activity) respectively. The optimum temperature for partially purified extracellular phytase was 40°C, and the optimum pH range for extracellular phytase activity was pH 6.5-7.0. The extracellu...

M. S. Bajestani1, K. Dolatabadi2†, E. Mahdikhani-Moghadam1

...iv>officinalis L.) and nigella (Nigella sativa L.) on root-knot nematode, Meloidogyne javanica @ 1500, 2500 and 5000
juveniles were studied on susceptible tomato cv. Karoon under greenhouse condition. Although all the treatments
reduced root infections rate and significantly effect on root weight (P ≤ 0.05). It was observed that when the
nematode inoculum increased, reproduction factor ...

M.A.M. El-Saedy, A.A. Mokbel*† and S.E. Hammad**

...mphor, castor, garlic, nigella, onion
and sesame resulted in 48.0-92.7% reduction in numbers of root galls/root under laboratory conditions. Similarly,
application of these oils resulted in great reductions of 59.2-92.8% and 47.4-89.6% in numbers of root galls and
egg-masses/root when oils applied before and after transplanting of tomato seedlings, respectively. The highest
reduction ...

O. A. Fabiyi

...romatography on a silica gel packed column. The nematicidal activity of the fractions were
evaluated in the laboratory using a 4x4x3 factorial experiment, while the crude extracts were tested on
Meloidogyne incognita infecting Abelmoschus esculentus in the field. Three hundred juveniles were used for the
assessment. Acetone extract coded DNLO/Me2CO caused a significant increase in plant height, number of leaves,
...

Tilahun Bekele* and Abebaw H. Georgis

...otypes of black cumin (Nigella sativa L.) in response to seed rate. The experiment composed of four varieties (Dirshaye, Darbera, Aden, and local), and four levels of seed rate (10kg, 15kg, 20kg, and 25kg per ha) set out in a randomized complete block design and replicated three times. The findings of the statistical analysis exhibited that the majority of the response variables under consideration were significantly (P<0.01) influenced by seed rate, variet...

O. A. Fabiyi, G. A. Olatunji and O. Atolani

...chromatography on silica gel (100-120 mesh grades) using glass column. The chromatographic fractions were tested in vitro along with their crudes and carbofuran on Meloidogyne incognita

Mohamed S. Abbas1*, Adel E.M. Mahmoud2, Hemat S. Mohamed3, Adam Cieślak4 and Małgorzata Szumacher-Strabel4

Hasnain Raza1,2, Qun Liu1*, Muhammad Tariq Hanif2 and Yanan Han1

... the models estimated largely similar results. The stock status results indicate that Euthynnus affinis and Rachycentron canadum were healthy, while Scomberomorus commerson and Parastromateus niger were overfished and Lutjanus argentimaculatus was strongly overfished. However, all the stocks were subjected to overfishing. Therefore, the parameters estimated from our study may be used as indicators for the sustainable management of the fisheries in the country....

Abdirahman Barre*, Karanja D Njuguna, Bebora Lilly Caroline and George Chege Gitao

...nt assay and double agar gel immunodiffusion test. The selected camels were followed into the slaughterhouse and pathological changes were identified grossly and microscopically based on alteration in organ and/tissue structure. The three main clinical signs that suggested brucellosis were lameness, swollen lymph nodes and abortion. Out of 160 samples tested, 15 (9.37%) were positive for Brucella antibodies and evenly distributed between counties; 8% (4/50) fo...
 
 USMAN AHMAD*1, AMTUL JAMIL SAMI1, MADEEHA KARAMAT1, MADEEHA KHALID1, FAIZAN NAEEM2 & MUHAMMAD JAMIL YOUSAF
...s of psyllium based hydrogels was observed. In this article we have fabricated different psyllium based dermal patches of hydrogels for insulin delivery. Psyllium, a natural polysaccharide, is a dietary fiber and widely utilized in gel forming. To study the structural aspects of these various polymeric webs were categorized with FTIR, SEM and release related experiments such as swelling ki...

E. K. Allam1; Kh. A. El-Dougdoug1; M. A. Amer2; A. A. Abou-Zaid2; and S. M. Amin2

...ng return-polyacrylamide gel electrophoresis (R-PAGE) and molecular techniques. The method involved the extraction of circular nucleic acids followed by electrophoretic analysis of the extracts on 5% polyacrylamide gel. A reverse transcription-polymerase Chain reaction (RT-PCR) assay was used for the isolation and identification of PSTVd cDNA from infected plant materials using a specific primer to PSTVd. A major cDNA produc...

 Salwa N. Zeinl and Hanaa S. Zawam2

..., and SDS-Polyacrylamide gels. Purification of ACLSV was performed using bentonite/polyethylene glycol. Electron micrograph of purified virus preparation revealed flexuous filamentous virus particles of 700 nm length and 12 nm wide. The average yield was 1.14-1.7mg/100 g tissue of Chenopodium quinoa Willd. Purified virus preparation was used for rabbit immunization. The polyclonal antibodies raised against the local isolate of ACLSV had a specific titer of l: ...

Saima Mehar1, Amtul Sami2 and Erum Iqbal3*

.... (Cuminum cyminum and Nigella sativa) in methanol, chloroform, ether, butanol, and hexane on egg hatching and mortality of the juveniles of root-knot nematode Meloidogyne javanica. After 72 hours, C. cyminum (MeOH) demonstrated the maximum nematicidal activity in egg hatching (15%±2.10) of M. javanica, followed by N. sativa (Ether), 22.33±6.86%, at the lowest administered dose of 0.25 % concentration. After 72 hours of treatment, C. cyminum (MeO...

Fakhra Nazir1*, Sahar Fazal1 and Fakhar-i-Abbas2

... confirmed by 1% agarose gel electrophoresis. Sequencing was carried out by Sanger’s method and BLAST analysis identified these samples as five species (Urocissa flavirostris, Dendrocitta vagabunda, Corvus splendens, Corvus corax and Corvus macrorhynchos) from three genera of family Corvidae. The nucleotide sequences were submitted to the Barcode of Life Data System (BOLD) and the eligible sequences were assigned the Barcode Index Numbers (BINs). The COI...

Amina Rahat1, Zahin Anjum1*, Sehlina Zehra2, Fatima Umal Baneen3 and Rabia Chishti1

...is also anticancerous. Nigela sativa is used for jaundice, paralysis, metabolic syndrome, insulin resistant syndrome, and high level of cholesterol, nerve tension problems; it stops hair fall and boost’s immune system. Based on the results of this study, it can be concluded that almost all the plant extracts showed potential antibacterial, insecticidal and antifungal activities against different. The research has focused on the potential therapeutic effi...

Muhammad Aijaz1, Nasir Mahmood2, Ghulam Mujtaba3 and Imran Riaz Malik1*

...were analyzed by agarose gel electrophoresis to detect polymorphism. Further analysis was done by sequencing the PCR products from some randomly selected samples. Urban locality, drinking of non-filtered water, poor hygiene, and poor socioeconomic status are the risk factors for the onset of breast cancer in the Pakistani population. Molecular analysis of the human IL-10 gene revealed that the prevalence of homozygous normal GG allele was 19%, heterozygous GA ...

Qi Zhuo, Yuanchun Yao, Meisongzhu Yang* and Jinhua Chen and Miao Tian

...lation mechanism of transgelin gene in colon cancer cells. HT29 cells were randomly divided into three groups: CON group, TGT group and DGT group. The transgelin gene was sub-cultured in the control (CON) group, transfected in the TGT (cells transfected with tranglin gene) group, and knocked out in the DGT (cells in which tranglin gene has been knowcked out) group. The protein expression and the gene expression were measured...

Zia Gul1, Muhammad Sajid1, Muhammad Noman Khan1*, Faheem ul Haq1, Zohra Nawaz1, Muhammad Bakhtiar2, Sajid Siddique1, Komal Aslam3 and Muhammad Irshad1

... of Aloe vera growth and gel production to organic manures and irrigation intervals was carried out at Ornamental Nursery, Department of Horticulture during the year 2014. The experiment was consisted of two factors, Factor A was irrigation intervals I1 =15 days, I2 =30 days and I3=45 days interval which was assigned to main plots, while Factor B was organic manures (control as O1, Leaf compost (LC) as O2, Poultry manure (PM) as O3 and Mushroom compost (MC) O4...

Kazi Afsana Homayra Orchy1, Mst. Antora Akter1, Nelema Yesmin1, Md. Moshiur Rahman Khan1, Marzia Rahman2, Md. Mahmudul Alam1* 

...th freshly prepared hPRP gel twice a week. The control rabbits received normal saline topically. The wounds were evaluated for 45 days post-burning. Morphological features of wounds such as pain sensation, the development of crusts, exudates and pus, and morphometric assessment like wound contraction were recorded. On the 7th, 14th and 21st days post-burning, biopsies were taken for histopathological assessment. In this study, the morphological and morphometri...
Tariq Ahmad1*, Anum Razzaq2*, Li Bo1, Faiz ur Rehman3, Gul Saba2, Omama Saqib1, Saif Ullah2 and Muhammad Suliman1
...complexity, affecting largely local communities that nearer to forests. Therefore, the two villages of District Dir lower (village Takoro and village Koherai Malakand), the part of great Himalayas has been selected to highlight the conflict between Himalayan palm civet (Paguma larvata) and local community during January 2018 to end of November 2020. During current studies it was found that the problem mainly arises due to civets foraging habits, who damages an...

Rashid Saraz1, Saiqa Amur1, Zia-ul-Hassan1*, Naheed Akhter Talpur1, Inayatullah Rajpar1, Muhammad Sohail Memon2, Muhammad Nawaz Kandhro3, Khalid Hussain Talpur1 and Nizamuddin Depar4

...int were recorded with Magellan® Triton™ 200 GPS device. Efforts were made to include all agriculturally important union councils of six talukas, involving a variety of soil use and crop scenarios, viz. wheat, sugarcane, mango, brassica. Soil texture (Bouyoucos Hydrometer method), electrical conductivity and pH (1:2 soil-water extract), organic matter (Walkley-Black method), and ABDTPA (Ammonium bicarbonate diethylene triamine penta acetic acid) extr...

Rida Ahmad1,2*, Zulfiqar Ali2*, Farkhanda Manzoor1, Usman Ahmad3, Safdar Sidra4, Irfan Zainab2, Muhammad Furqan2, Aliza Batool1,2 and Zaidi Zona2

...pecies was maximum in rangelands (0.82) even though it was also observed in six habitats. Furthermore, Western Tragopan was found only in moist temperate habitat with HSI 0.70. The current study revealed that suitable habitat of these species is shrinking mainly due to habitat loss, its fragmentation and hunting pressure. Species prefer habitat with specific characteristics and this paper provides recommendations for the conservation and management of Steppe E...

Jameel-ur-Rehman1, Asghar Ali Kamboh1*, Ahmad Ali Moryani1, Riaz Ahmed Leghari1, Abdullah Sethar2, Ali Raza Nizamani3, Abdul Ahad Soomro3, Kanwar Kumar Malhi1 

... (78.3% & 58.3%), Shigella flexneri (61.6% & 53.3%), Pseudomonas flavescens (55% & 53.3%), Pseudomonas aeruginosa (40% & 35%), Staphylococcus aureus (33.3% & 25%), Streptococcus agalactiae (21.6% & 18.3%), Klebsiella pneumoniae (15% & 13.3%), Staphylococcus epidermidis (13.3% & 10%), Klebsiella oxytoca (10% & 8.3%), and Streptococcus pyogenes (6.6% & 1.6%) in market and pond fish samples respectively. According to anti...
Burarah Arooj1, Zeeshan Mutahir1, Moazzam Ali1, Mohsina Akhter2, Malik Siddique Mahmood1, Arslan Hamid3 and Mahjabeen Saleem1*

Olabode, H.O.K1, Mailafia, S1, Martha Echioda-Ogbole1*, Ameh, J.A1, Amadi, K.I1 and Meseko C.A

Blessing Ndenum Peter1, Olufemi Bolarinwa Adedeji1, Reuben Chukwuka Okocha2,3*, Ekemini Moses Okon4 

... spp. from 68(85.0%), Shigella spp. from 52(65.5%), Bacillus spp. from 52(65.5%), Staphylococcus aureus from 56 (70.0%) and Staphylococcus epiderdimis from 43 (53.8%) samples. All the Salmonella and Shigella isolates were sensitive to ceftazidime, cefuroxime, ofloxacin, and ciprofloxacin but resistant to gentamicin, cefixime, augmentin, and nitrofurantoin. This study concluded that ready-to-eat shrimps in Ibadan are not safe...

H.A. El-Nagar1, A.M. El-Hais2, M.S. Mandouh2, W.M. Wafa1*, A.H. ABD El-Aziz, K.A. Attia4 

...), black seed oil (BS, Nigella sativa), or their combination on growth performance, immunity, and health status of females of newborn Friesian calves. A total of 20 newborn calves (37.51±0.92 kg LBW) were divided into 4 groups. During the suckling period, animals in the 1st group were fed a starter without any addition (G1, control). Animals in G2, G3, and G4 were fed the same starter supplemented with BP (10g/kg), BS oil (10g/kg), or their combination ...

Ahmed Al-Hasnawi*, Wafaa Kadhim Jasim

... administrating Aloevera gel extract (500mg/kg b.w). (G4) group: were administrating azathioprine (50mg/kg. b. w) and aloe vera gel extract (500mg/kg b.w). after four weeks, blood samples from the rats were taken to measure MCV, MCH, MCHC, GST, GSH, and MDA levels. a liver sample was also taken for each of the aforementioned groups to be examined histopathological. According to the findings, azathioprine dramatically decreas...

Abida Mushtaque1, Ali Ahmad Sheikh1, Aamir Ghafoor1*, Wasim Shehzad2 and Nadeem Ahmad3

...-negative, small, non-flagellated, pleomorphic coccobacillus. It does possess different types of outer membrane proteins (OMP’s) which assist in interaction with host cells. The outer membrane protein H (OmpH) of Pasteurella multocida B:2 is a major transmembrane porin that can be used as a subunit vaccine and development of diagnostic kit against hemorrhagic septicemia (HS) because of its immunogenic nature. Pasteurella multocida has economic importance...
Omaima Khamiss
...hybridization dot blots, gel transfer to follow the probable mechanism especially with the high percentage of genome homology that was over 66% with one of five tested restriction enzymes. These observations suggest an important possible role for recombination in the early evolution and biological characteristics of these two viruses.
...

Rizk, Sonia A.; El-garf, Eman M. and Talaat, Abeer A.

...n the immune response of gel adjuvanted Bivalent Foot and Mouth Disease (FMD) vaccinated calves. This study conducted in two calve groups; goup (A) vaccinated subcutaneously with bivalent Alhydragel adjuvanted (30) % FMD vaccine, while group (B) vaccinated subcutanously with bivalent FMD vaccine adjuvanted with both Alhydragel and GSLS (10 mg/dose). The humeral and cellular immunoresponses...

Sofyl *, A.R.; Soliman , A.M.; Mousal, A.A. and El-Dougdoug , Kh.A.

...rk agent was detected by gel electrophoresis. The expected size of amplified cDNA by RT-PCR was approximately 300 bp. A phylogenetic tree of the Egyptian citrus gummy bark agent (Accession no. FJ984562) revealed 100%, 99.7% and 97.8% a moderate degee of similarity to CVd-11b (USA), CCaVd (Spain) and CCaVd (Egypt) respectively. The minimum free energy of a secondary structure for HSVd-EG-RNA was determined using its primary sequence at 370C. The sequence appear...

El-Dougdoug, Kh.A.; Rezk , A.A.; Dawoud, Rehab A. and Sofy, A.R.

...ecific primers. Purified gel RT-PCR product (⁓199) was cloned into the PCR Il TOPOvector then it was sequenced. Partial sequence 199 bp of CSVdEG is almost identical to that of the prototype 199 bp Canada and USA isolates of CSVd with 96% homology. The sequence of CSVd-EG can be arranged into viroid specific rod like structure. CSVd-EG differ from the prototype isolates Canada and USA at sites occur in regions corresponding to the conserved, variable and rig...

El-Tabakh, SAA l ; Abdel Wahab, KSE; Badr, AF   and Helal, IG  

...oteins by polyacrylamide gel electrophoresis (PAGE). HCV infected and control HepG2 cells supernatant fluids (SF) were sampled before complete change with fresh MM at weekly intervals for periods extended to one month after infection. Three SF samples taken 5 days apart after HCV infection showed that detection of HCV-RNA in SF was intermittent but' detection of new native protein as well as glycopeptides was consistent.  
...

Eman, Abo Hatab*; Hussein, HA.; El-Sabagh IM. and Saber, MS.

... eluted from the agarose gel and sequenced using VP7 specific primers sequence. The obtained sequence was analyzed using computer software (BLAST) which revealed that both isolates had Maximum identity to the GIO serotype of group A bovine rotaviruses ranging from 90-93%. This is the first report on the circulation of G 10 serotype of group A rotaviruses in camel. 

...

Amer, H.M.; Hussein, H. A.; El-Sabagh, I. M.; El-Sanousi, A. A. Saber,M.S. and Shalaby, M. A.

...s cut and extracted from gel using DNA gel extraction kit (Millipore). Eluted DNA was successfully cloned in pBlueBac4.5N5-His TOPO TA baculovirus transfer vector and transformed in chemically competent E. coli. A modified colony PCR assay was utilized to identify the positive bacterial colonies that harbor the recombinant plasmids carrying N gene in correct orientation. Generation of recombinant baculoviruses was achieved b...

Sonia, A.M.; El. Sanousi, A.A.; Saber, M.M.; Daoud, A.M.; Samira, E.K.; and Ismail I.

... compared with Al (OH) 3 gel vaccine and tested in calves. The obtained results revealed that the duration of immunity elicited by gel FMD vaccine was shorter than oil adjuvanted FMD vaccines. Results also indicated that vaccine emulsified with Montanide IMS 3015 could elicit the best protection capability with long lasting immune response (up to 42 week) in calves, if compared with other FMD vaccine batches emulsified with:...

Abdel Razek,B.Omar*and Magda M.Sayed**

...sts were applied by agar gel precipitation test (AGPT) and indirect fluorescent antibody technique (IFAT). Serum neutralization test (SNT) and ELISA tests were applied for titration and evaluation of the hyperimmune sera before conjugation with fluorescent. Total protein concentration of the prepared LSDV antisera was 0.8g/dl. Separation of anti-LSDV immunoglobulins 1gG were done using ammonium sulphate followed by conjugation with fluorescein isothiocyanate a...

H.A. SULTANl, H.A. HUSSEIN2, and F.F. EL-KHAYAT3

...ffected birds using agar gel precipitation test (AGPT) and antigen capture- enzyme linked immunosorbent assay (AC-ELISA). Antigenic typing of IBDV from selected outbreaks were carried out by monoclonal antibodies-based AC-ELISA and the pathogenicity of some representative infectious bursal disease virus (IBDV) field isolates were studied. Of the selected 22 IBDV -positive bursal samples, 59.1% (13/22) were typed as classic IBDVs and 40.9% (9/22) were of varian...

Musaad A. Al-Dubaib

...ent animal species. Agar gel immunodiffusion was carried out using commercial diagnostic reagents. All samples of herds having positive cases with immunodiffusion were tested by ELISA. Positive cases were recorded among samples of the Holstein cattle with both serological tests. Prevalence rates differed from one farm to another and with both serological tests. With agar gel immunodiffusion, the prevalence rate in 3 farms we...

Saddam Hummadi1, Nadia Al-Falahi2* 

...ynthesis and use of hydrogel derived from small intestine submucosa (SIS) and AgNPs composite for accelerate the healing of infected wounds and improve cosmetic outcomes. A 5% w/v SIS hydrogel was prepared and formulated with 100 µg/ml AgNPs to evaluate its effect on healing of infected wounds in rabbit. Forty eight adult rabbits aged 8-12 months, weighing 1.5-2.5 kg, were divided randomly into three equal groups (n=1...

Muhammad Talha Sajjad1, Hamid Akbar1*, Muhammad Arif Khan1, Muhammad Hassan Mushtaq3, Shehla Gul Bokhari1, Muhammad Abid Hayat4 and Ghulam Mustafa2

...atelet-rich plasma (PRP) gel has been suggested for wound repair. However, there is insufficient evidence to support its usage in animals with cutaneous wounds. Therefore, this study aimed to evaluate the effect of PRP gel on cutaneous wound healing in rescued horses. Twelve horses were divided into two groups: group A (PRP-treated) and group B (control). The group A (n=6) animals were treated with autologous PRP

Phoebe Lyndia Tolentino Llantada1,2*, Midori Umekawa2, Shuichi Karita2

... PCR-denaturing gradient gel electrophoresis (DGGE) method to profile the bacterial community and PCR amplification using species-specific primer sets for 16S rDNA fragments to detect major fibrolytic and non-fibrolytic bacteria. Results from the PCR-DGGE analysis showed differences in bacterial diversity, density, and banding patterns along the gut of buffaloes. Higher bacterial diversity, density, and banding patterns were observed in the foregut and hindgut...

Shahid Iqbal1, Arshad Khan, Ali Hazrat1*, Gul Rahim, Mohammad Ihsan1, Umar Zad Gul1, Maryam Bibi1, Khadija Bibi1 and Muhammad Mukhtiar2

...s using 12.5% acrylamide gel, where a total of 14 polymorphic bands were observed. In Band 1 the highest degree of variation (0.83%), followed by Band 2 and Band 3 with a value of 0.75% variation, whereas the lowest (0.17%) was found in Band 12, followed by Band 14 with a value of 0.25% respectively. Based on two-way cluster analysis all the genotypes were divided into 2 main groups and further subdivided into 6 groups where the genotypes RC01 and RC16 were fo...

Aniqa Qaisar, Shabana Naz* and Sajida Arooj

...NA damage by single-cell gel electrophoresis (comet) assay. P. cristatus kept at Wildlife Park Murree and Jallo Wildlife Park Lahore had almost same and greater DNA damage than the birds kept at Wildlife Park Bahawalnagar. Heavy metal concentrations and variation in DNA damage were correlated. Only Cr and Mn concentrations were directly associated with the LHead and LComet variations. This study concluded that heavy metals contamination exposure could be detec...

Wafa’a Mohammad Abduwhab1*, Waseem Ali Hasan2, Mohanad Abdulsataar AL-Bayati3

...tosome pumpkin-lidocaine gel in the context of third-degree burn wound healing. The reformulation of standard pumpkin seed oil involved the encapsulation of conventional Lidocaine within a phytosome structure, resulting in the formation of nanoparticles. This modification was undertaken to enhance both the physical stability of the oil and the therapeutic properties of pumpkin seed oil, while simultaneously mitigating the potential side effects associated with...

SYED MUJTABA HAIDER1, KHIZAR HAYAT BHATTI1*, EJAZ HUSSAIN SIDDIQI1, SYED MAZHAR IRFAN1,`2, & ALLAH BAKHSH GULSHAN2

...reptococcus pyogenes; Shigella sonnei; Escherichia coli (1) (4C11); Escherichia coli (2) (ATCC 13048) and Neisseria gonorrhoeae). Agar well diffusion method was applied to assess the antibacterial activity of these extracts. Their antibacterial activities were associated with typical chemical like gentamicine (antibiotic) as positive control. This study revealed that S. pyogenes, S. aureus and N. gonorrhoeae had the maximum inhibition to different extracts of ...

SYED SHAHID IMRAN BUKHARI1*, IQRA AZAM1, MUDDASIR HASSAN ABBASI1, MEERAB DAUD1, NADEEM SHEIKH2, ANDLEEB BATOOL3, RABIA MAHMOOD2 & MARYAM MUKHTAR2

...mples were processed for gel run and thermo cycler program, which revealed alterations in the expression of hepatic mRNA (IL-6) in all loads equally confirmed by the results of housekeeping gene β-actin. Gel analysis showed changes in the folds of IL-6 in hepatic mRNA compared to control. These finding led to the conclusion that Alloxan can induce an APR, which is evident by rise in expression of IL-6. Hence, extensive ...

MUBEEN RIAZ1, KIRAN ZAHID1, SUMAIRA ASLAM CHOHAN1, MUHAMMAD ASHFAQ*2, MUHAMMAD ALI2, RIFFAT SIDDIQUE1, NUDRAT ANEES1 & FARAH KHAN1

...fication was followed by gel electrophoresis (1.2% agarose gel) and visualized by UV light, which revealed the presence of monomorphic and polymorphic bands in the amplified products. During this analysis, two primers (OPH_01_F and OPH_01_RC) produced a total of 10 bands. Six of the bands were monomorphic i.e. 02 with the control plant, 02 with the 25mM salt stressed plant and 02 with the 50mM salt stressed plants. While wit...
SHAHZADA NADEEM ABBAS1, KIRAN JAMSHED1, SARAH BUSHRA NASIR2,
SAMAN SHAHID3 & NASIR MAHMOOD4
...methanolic extracts of Nigella sativa and Ficus carica seeds were used against bacteria (Staphylococcus aureus and Escherichia coli) and Fungi (Candida albicans, Fusarium oxysporium and Microsporum canis) to check their antimicrobial activities. For antibacterial assays, the volume and concentrations of extracts used were 0.5 μl (62.5 μg), 1 μl (125 μg), 5 μl (625 μg) and 10 μl (1250 μg). Specific laboratory protocols of antibacterial a...

SHEIKH AJAZ RASOOL1, MUHAMMAD SALMAN RASOOL2 & MUNAZZA AJAZ3

...mulation and directed flagella based shifting resulting out of hypo-doses of drug stimulation. Further, the prevalence of Enterobacteriaceae member strains has been witnessed producing Extended Spectrum β-Lactamases (ESBLs) and Carbapenemases. Drug resistance in viral entities has also posed challenge for public health programs. About 20% people die of viral hepatitis in one of Pakistan provinces. A counter malarial acrine drug is being tried against prot...

Ngoc Tan Nguyen1*, Thi Tuong Vi Trang1, Thi Thuy Tien Nguyen2, Tuan Thanh Hoang2, Duc Thoa Nguyen2

...rophoresis on 2% agarose gel for genotyping. Polymorphism was observed at the 24 bp PRL/indel locus, showing two alleles I and D with frequencies 0.57 and 0.43, respectively and three genotypes (II, ID, and DD) with frequencies 18.3, 49.4, and 32.3%, respectively. Hens with the DD genotype had a significantly (P<0.05) higher ANE than hens with the ID or II genotypes. The regression equation, Y = 1.5437X + 58.386 (R2=0.0185) showed a positive correlation wit...

Nabaa Fadhil Abbas*, Raffal A. Omar

...ceived remifentanil in a gel formulation. After local administration of these substances by infiltration (s/c) and compared with the control group. the severity of the burn injury and subsequent healing were assessed from first-degree burns. The study results revealed that tramadol and remifentanil with epinephrine significantly slowed down the wound-healing process in rabbit burn models compared to the other groups studied. This study investigated the effects...
Mouhamed Zakiou Kolawole Adissa Raimi1, Ghulam Hussain2, Nousheen Zafeer3, Faheem Ahmad1, Muhammad Irfan4, Anwar Ullah1, Imdad Kaleem1 and Asghar Shabbir1*
...e effects. Blackseeds (Nigella sativa) and honey possessing potent neuroprotective, antioxidant, and anti-inflammatory properties with no reported side effects can be a prospective candidate for an alternate remedial treatment of MS in animal model as well as in humans. In this study we established a new animal model (quail), to assess the synergistic efficacy of honey and black seed against demyelination within brain. A total of 35 male quails were used, amon...

Aspen Abutalip*, Batyrbek Aitzhanov, Assiya Mussayeva, Vladislava Suchshikh, Natalya Yegorova

...s associated with the flagellar antigen Clostridium chauvoei were used, which sensitised latex microparticles with a size of 0.8-1.1 microns from Thermo Fisher Scientific Corporation. In the process of testing the prepared diagnostic system with vaccine strains of the causative agent of emphysematous carbuncle used for active immunisation of animals in Kazakhstan and field experiments with biological material from sick and deceased animals, results were obtain...
Kamran Ahmed Pathan1 , Waheed Ali Panhwar1,*, Abdul Manan Shaikh1, Safdar Ali
Ujan1, Javed Ahmed Ujan1, Khadim Hussain Memon1, Irfan Ahmed Pathan1 and
Shabana Mangi1
...rp, 1905), Dorysthenes hugelii (Redtenbacher, 1848) were
reported first time from Pakistan. Additionally, the association of various plants with the
species is provided for the first time. This study will be a baseline for the future research
associated with longhorn beetles.
...

Nittaya Thongtip1, Sukanya Poolthajit1, Sirikhan Thartrak1, Qiongxian Yan2, Suntorn Wittayakun1* 

...atments were provided in gelatin hard capsules prepared for each treatment except the control. All animals were individually fed roughage to concentrate at a ratio of 30 to 70 daily. The experiment consisted of four consecutive periods, with the first 14 d for dietary adaptation and the last 6 d for data collection each, and lasted for 80 days of overall trial. Results indicated that all supplementations did not affect feed and nutrient intake (p > 0.05). H...
Hasina Baloch1, Rahmatullah Rind2, Dildar Hussain Kalhoro1*, Shahid Hussain Abro1, Hubdar Ali Kolachi3, Saqib Kakar1, Muhammad Ibrahim3, Fayaz Ahned1, Hasnain Ali1 and Muhammad Mahesar1
...species caused intensive gel formation in subclinical mastitis. However, 840 quarters were found with infection at diverse degree. Only 102 Most severe (4+) samples noted strong gel as with mean somatic cell count (SCC) of 8,1x105 while 60 more severe (3+) samples showed distinct gel with mean SCC of 2,7x105. Among the 102 strong positive samples, 57 (17.27%) contained pathogens. The corre...

Ashar Farooq1*, Farrukh Waheed1 and Asad Abbas Khan2

...ing capacity in scrub rangeland of Kherimurat, Punjab. For field data collection, two homogeneous sites i.e., grazed and un-grazed were randomly selected in Compartment No.12 of Kherimurat, scrub rangeland. Reconnaissance of the study area was carried out to layout the transect lines and quadrat method was used to collect the vegetation parameters such as cover percent and forage production. Three transect lines, each of 100...
Nancy Shackelford , Michael Renton , Michael Perring , Kristine Brooks , Richard Hobbs
Ghayyas Ahmad1, Arifullah Awan2 and Muhammad Imran3
...od. Local people were largely unaware of its legal status or lopping's adverse affect on the tree's health. Axe was the main tool used for lopping. The preferred species was Oak but since its availability was scarce, people had no choice but to lop Chir pine. As per local customs, women played no role in this activity....
Muhammad Farooq1*, Lal Badshah2 and Sanam Zarif1
...rea. The investigated rangelands are less productive and need rehabilitation through ecological management. This would be possible with participation of local communities and NGOs to make the plant resources sustainable. There is a dire need to provide an alternate source of energy to overcome the burden on rangeland resources.
Key words: Spectrum, Rangeland, Life form, Leaf size, Ashk...
Muhammad Yousaf Khan and Raees Khan
...nd bushes 533,523 ha, rangeland 794,471 ha, snow and glaciers 813,530 ha, horticulture is 15,519 ha, barren land (desert) is 646,305 ha, settlements is110,536 ha, and water bodies is 151,688 ha (Land Cover Atlas of Pakistan PFI 2012). Forest is the main revenue source of provincial exchequer till imposition of ban on forest harvesting in 1993 forest was contributing 43% revenue to provincial exchequer. Forest has the tremendous potential and scope for employme...
Asad Abbas Khan1, Amina Batool2, Muhammad Aslam1, Muhammad Ehtesham Asghar3, Abdul Ghafoor1 and Muhammad Arif1
...onditions of Pothowar....
Asad Abbas Khan1, Amina Batool2, Muhammad Aslam1, Muhammad Ehtesham Asghar3, Abdul Ghafoor1 and Muhammad Arif1
...onditions of Pothowar....
Muhammad Farrukh Lateef1, Asad Abbas Khan2 and Muhammad Arif
...poor condition of the rangeland could be improved by reseeding techniques. ...
Ashar Farooq1; Farid Shah2; Saifullah Zahri2 and Samiullah Jaffar2
...of arid and semi-arid rangelands of Balochistan. Six (06) species of Seriphidium are found in this region. The dwellers of the area are dependent on livestock to earn their keeps and livestock is dependent on these rangelands. Being a high palatable species, Seriphidium is the main target of large mammals. The experimental work has been carried out in the Maslakh protected area by comparing the cover, height, d...
Mubashir Jamil Khan1, Asad Abbas Khan2, Amir Saleem1, Lateef M. A.1, Muhammad Aslam1, Muhammad Mushtaq2, Abdul Ghaffoor2 Amna Btool1 and Arsalan Ali Khan1
...species of Kherimurat....
Sarfraz Hasan Dogar and Ashar Farooq
...d in Rakh Shadan Lund rangeland of Dera Ghazi Khan during October, 2010 for comparison of forage production & carrying capacity in seeded & unseeded range areas. Reseeding was done during 2009-2010. Data were collected by laying out eight transect lines each 30 meters long, four each in seeded and unseeded areas. Forty quadrats (1x1m) i.e. 20 in seeded area and 20 in unseeded area were studied for forage yield determination. Data were statistically analyzed us...
Tariq Mahmood
... land use followed by rangelands (23.80%), shrubs & bushes (22.32%) and agricultural land (19.94%).The change detection result shows that there is a considerable increase in Shrub & Bushes (21.33%), followed by Forests (9.76%). It also indicates a considerable decrease in land cover class agriculture land (28.34%), followed by the Rangeland land cover class (4.83%).

Key words: GIS/RS, Forest covers change assessment,...

Ashar Farooq and Mubashir Hassan
...from Rakh Dagar Kotli rangeland of Thal desert to determine the plant species composition, forage production and carrying capacity of the area to evaluate the current condition of the rangeland. Systematic sampling with random start procedure was adopted to collect the data. The study revealed that species composition of Cenchrus ciliaris was maximum (42.08%) followed by Eleusine flagel
Ch. Muhammad Muslim and Sohail Sikander
...cuminata, Rheum emodi, Angelica glauca, Adiantum capillus, Dioscorea deltoidea, Digitalis spp., Berberis lycium, Geranium wallichianum, Berginia ciliata, Solanum nigrum etc., for supply of quality seed and planting stock for other areas. Medicinal plants are a valuable natural resource and regarded as future of safe drugs. Sustainable management of this renewable natural resource is an important issue which needs to be discussed at national level. B...
Ch. Muhammad Muslim1, Yahya Karamat2, Muhammad Shabbir Mughal3 and Imtiaz Hussain4
... natural resources. Nigella sativa is a renowned medicinal herb of the world of herbaceous plants because of its multipurpose uses in different disease treatments. The study was conducted to increase the production of Nigella sativa by applying the NP fertilizer. This study was carried with objective to find the optimum doze of NP fertilizer for the growth and maximum yield of Nigel<...
Shakeel Haider Zaidi, Ch. Muhammad Muslim, Muhammad Shabbir Mughal and Imtiaz Hussain
...its psoralen content, largely used in psoriasis and certain immune diseases. To assess the possibility of its introduction and cultivation as a specialized crop, yield trials with different levels of nitrophos fertilizer (20:20 NP) on the seed yield were conducted for three consecutive years at Medicinal Plant Farm of Pakistan Forest Institute, Peshawar. The plant has successfully acclimatized under climatic and edephic conditions; gave better growth and highe...
Malik Mahboob ur Rehman1, Muhammad Rafiq2, Amjad Ali Ch.3, Tariq Mahmood4, Javaid Ahsan5 and Shahzad Fazal6
...eas while Eleucine flagellifera (Chimber) (38.72%) in untreated areas. General grass coverage on the average was 9.7% and 36.75% in un-treated and treated pastures respectively. Carrying capacity based on dry biomass of grasses/herbs was found to be 18 Ac/AU/Yr and 10 Ac/AU/Yr in un-treated and treated areas. Study concluded that untreated area provides fodder to 944 Animal Unit/5664 Sheep Unit while that of treated area to 1700 Animal Unit/10200 Sheep ...
Muhammad Afzal, Amjad Ali Ch., Javaid Ahsan, Shahzad Fazal and Saima Tabassum
...urvey of Pabbi-Rasool rangeland was conducted to assess its vegetation and carrying capacity. Cenchrus ciliaris (Dhaman) was the principal grass sp. (47.85%). General grass coverage on average was 57.01% and 30.27% in protected and open pastures respectively. Dry matter yield of protected pastures was 1316 kg/ha while it was only 388 kg/ha from open pastures. Carrying capacity based on herbage biomass for protected and open pastures was found to be 0.40...
Mohammad Athar and Syed Mahmood Nasir
...ly has spread from Los Angeles to 37 counties, including all of the state's commercial olive growing areas. Olive fruit fly was trapped from 19 tree species belonging to nine genera distributed in seven families of angiosperms. Olives (Family Oleaceae) were the preferred host of the olive fruit fly. Family Rosaceae has nine tree species followed by Rutaceae (five tree species) as host of olive fruit fly. Other host tree species were distributed in Anacardia...
Amjad Ali Chaudhry, Chaudhry Muhammad Muslim and Muhammad Mushtaque
...er, 2000. Eleusine flagellifera was the key grass species. 26.37% and 16 88% ground was covered by grasses/herbs and shrubs, respectively while 56.75% was uncovered. Carrying capacity was found to be 1.003 AU/ha/yr and shrubs were the major contributors of forage. The Rakh presented miserable condition of grasses and it is strongly recommended that rotational system to be followed which promotes forage vigour by avoiding repeated and continuous grazing of o...
Klaus Euler and Zulfiqar Ali Qazi
... forests, 24.2% under rangelands, 39.4% under wastelands, 7.6% under water bodies and permanent snow, while 1.4% of its area is under agriculture. Livestock rearing and subsistence agriculture is the mainstay of the local economy With about 32,000 livestock heads, there is a tremendous pressure on the rangelands Reduction in the numbers, better breeds, stall feeding, better marketing facilities, awareness raising, etc. are s...
S. M. Rafique
... state of art of arid rangelands, their management constraints and scope of range vegetation evaluation. The results of a case study on Lachi rangelands indicated that the area was rich in plant bio-diversity and there were 36 plant species. Out of it 18 were grass/grazing species, 2 forb species, 10 shrub species and 6 tree species. The bio-mass production was 752 kg (AD)/ha which was quite low. The study further indicated ...
Mahmood Nasir and Mohammad Noor
... protect family owned rangelands in summer each year by constructing loose stone walls for free grazing during winter In October 1995, species wise cover percent, density and above ground biomass data were collected to detect vegetation differences between protected and unprotected area. Twenty 1 m2 quadrats were studied randomly in both the areas.

The cover percent (10%) and above ground biomass (216 kg/ha) of Chrysopogon aucheri were higher thr...

Editor
... Forests, Watersheds, Rangelands, Wildlife, Biodiversity and their habitats. The policy seeks to launch the active participation of all the concerned agencies and stakeholders, to realize the sustainable development of the resources. It is an umbrella policy providing guidelines to the Federal Government, Provincial Governments and territories for the management of their RNR. In consonance with it the Provincial and District Governments may devise their own po...
Amjad Ali Chaudhry, Muhammad Afzal, Muhammad Mushtaque and Barket Ali Awan
...mportant Rakh of Thal rangelands. The current study conducted in this particular Rakh revealed a small potential of supporting only 0.177 animal units (AU) per hectare per growing season on a re-seeded and protected pasture. Cenchrus ciliaris stood at the top of the palatable species (24.80%). The other species included Eleucine flagellifera (27.52%) and Elionurus hirsutus (13.85%)....
Syed Zainul Arifeen and Abdul Khaliq Chaudhry
... such as agriculture, rangeland, forests and mixed forest, horticulture and agriculture in moist temperate zone at Chikar. Azad Jammu and Kashmir (AJK). In this study four sub-catchments were selected. Nine year's (1988-1996) average data showed that both the hydrologic response (16%) and sediment concentration (158 kg/ha) were minimum i.e. for the subcatchment under forest and was maximum i.e. hydrologic response (26%) and sediment yield (332 kg/ha) for the c...
Sardar Muhammad Rafique
...d that poor temperate rangelands could be rehabilitated by application of suitable fertilizer and control grazing for about 5 years....
Maqsood Ahmed and H. Verplancke
...ymers are water storing, gel forming soil conditioners, used to improve the water storing capacities of the drought-prone soils and to improve the supplies of plant available water, thus helping in better plant establishment and growth. The effects of polyacrylamide co-plymers on germination and growth were investigated in this study. The polymer was mixed with dune sand at different percentage by weight and soil-hydrogel
Wali-ur-Rehman
...f a medicinal plant Nigela sativa in the medicinal plant garden at the Pakistan Forest Institute, Peshawar during 1993. It caused complete seed losses in 73% of the infested fruits. In rest of the 27% fruits seed loss rated as heavy (8%), medium (8%) and Light (11%)....
Udo Schickhoff Dr.
...forests into farm and rangelands can be differentiated into several periods with varying degrees of intensity. It turns out that the dynamics of landscape change are heavily dependent on the general socio-economic conditions. Until the beginning of the nineteenth century the Kaghan Valley was very thinly populated. Substantial changes in the distribution of forests and agricultural lands occurred in the first two decades of British rule from 1847 to 18...
Mohammad Saleem and C. A. Call
...itions on Baluchistan rangelands. Both species were grown in monoculture and in a 50:50 mixture in an 11-month (44-week) greenhouse study. Defoliation treatments were implemented when plants were 32 weeks old; and consisted of: equally clipping (3-cm stubble height) plants in monoculture and mixture zero, one, two or three times at 4-week intervals (32, 36. and 40 weeks after emergence), and clipping (3-cm stubble height) one species in mixture zero, one, tw...
Javed Afzal, Abdul Sattar Alvi and Sarwat Naz Mirza
...nomic return. So, the rangelands are a valuable natural resource and need to be managed properly to get sustained maximum forage and ensure plant cover to halt soil erosion and further loss of primary productivity....
Mohammad Saleem and C. A. Call
... on arid and semiarid rangelands in Baluchistan. A controlled environment study was conducted to determine differences in leave and tiller development and root development between seedlings of Chrysopogon aucheri (Boiss.) staph, and Cymbopogon jwarancusa (Jones) Schult, at 15, 30, 45, and 60 days after emergence. Chrysopogon aucheri seedlings generally had greater, but not significantly greater, tiller and leaf development ...
Shakeel Haider Zaidi and Anwar Ahmad Khan
...ory properties and is largely used in indigenous and allopathic medicines. To assess the possibility of its introduction and cultivation as a special crop, yield trials with various spacings were conducted for 3 consecutive year (1989 to 1992) at Medicinal plants farm, Peshawar. The plant has successfully adapted to the climatic condition of Peshawar giving the highest yield when planted at 60*30 cm spacing. It can be easily cultivated under irrigated...
Mohammad Noor, Mohammad Khan and Gul Nabi
...ovement of overgrazed rangelands and secondary succession....
*Nasir M. But, **Noor Mohammad, *Sartaj and *M. I. Sultani
...um (A146) in the rangelands of Pothwar tract of Pakistan....
S. H. Iqbal, Shahbaz and Ghazala Nasim
...phedra gerardiana, E. regeliana, Juniperus communis, Pinus helepensis, P. roxburghii, Podocarpus chinensis, Taxodium mucronatum, Thuja orientalis and Zamia floridana. Endomycorrhizae were prevalent in species of most families including Pinaceae which normally forms the ectomycorrhiza. Fungal hyphae colonized the young roots and formed coils, arbuscules and vesicles inside the cortical cells. Coiled and linear hyphae were very common, digesti...
S. M. Chughtai, Jehandar Shah, Syed Zahir Shah and Sardar Hussain Shah
...s of early colonizers largely depending upon the availability of moisture and the coarser fraction of soil. In moderately wet new alluvial lands, Saccharum spontaneum was the leading dominant; on relatively drier sites, Astragalus graveolens took the lead over; and in more wet habitats, Salvia-Polypogon community dominated the scene. The stable land was dominated by Cynodon-Imperata community representing a relatively a advanced stage of success...
Sultan Maqsood Khan
...is a great variety of rangelands including foot-hill ranges, dry temperate ranges and alpine pastures. In cultivated fields shaftal, Lucerne, aftermath of agricultural crops, fodder trees and leaf fall of fruit trees are important sources of livestock feed. The availability of winter feed is a limiting factor in the production of livestock. In Northern Areas 100,125 metric tonnes of air dry feed is available annually against a requirement of 1,212,439 ...
Sultan Maqsood Khan and Raja Muhammad Zarif
...ying capacity of this rangeland from about 23 to only 0.5 hectares/Animal Unit/year. This shows that at present level of livestock management the production of livestock and livestock products can be increased about 40 times by simply seeding these areas with Cenhrus ciliaris. ...
G. M. Khattak, Mahmood Iqbal Sheikh and Abdul Khaliq
...tro: Pakistan is largely an arid and sami-arid land with two thirds of it under mountains and deserts. Agriculture is practiced over about 47.5 million acres, of which about 33.5 million are irrigated, mostly by canals. Production forests cover barely 1.5% of the total land area. So the country is short of timber and fire-wood: Wood and wood products - mostly pulp and paper - worth 550 million rupees are imported every year on the average. And the scarcit...
Sultan Maqsood Khan
...tones from cropland, rangeland and forests combined. The production of Shaftal can be increased by 90 thousand tones if the local variety is replaced with improved variety and fertilized. Planting of summer legumes in 10 per cant of the cultivated land and alfalfa under apple trees can increase the forage production by 772 and 40 thousand tones respectively. Replacement of undesirable forage species on field boundaries and range fertilization can furth...
M. Bashir Chaudhry (Dr.), M. Alim Mian and M. Rafiq
...chieved but these are largely negated by the sodicity problem caused by low-quality tubewell water. This kind of sodicity is affecting about half of the area irrigated by tubewells. Towards the end, priorities for agricultural development have been suggested, keeping in view the kinds and extent of the saline and waterlogged soils, the vast extent of high-potential but under-utilized agricultural land the economics of water use in the country. The sore need to...
Sultan Maqsood Khan
...ion cover in depleted rangelands of Mangla Watershed through introduction of suitable grass species is important for the production of good quality water and for increasing effective life if the Mangla reservoir by retarding its siltation rate. To find out suitable species for introduction in the Mangla watershed project area (dry sub-tropical) grass introduction trials with 39 cotypes of 14 species are being carried out in collaboration with Watershed Managem...

Md. Maniruzzaman1, Md. Kamrul Haque1,3, Md Rokonuzzaman2, Md. Mahmudul Hasan3, Rumana Biswas4, Md. Mustafizur Rahman5, Tahmina Akter Rimi6, Md. Rahat Uz Zaman7*, Md. Alauddin8, Md. Abdul Baki9 and Md. Yeamin Hossain10

... sector but remains a largely untapped area that has not developed properly, considering the country’s prospects in this particular field. With few exceptions, the agro-industrial sector of Bangladesh remains underdeveloped, and mainly without significant institutional, technical and financial support. Still, the size of the agribusiness sector in the domestic economy is relatively small, and the country’s total exports are also negligible. This st...
Fangmei Zhang1,2, Xiaocen Zhao1,2, Li Qiao1,2, Shibao Guo1,2, Li Zhang1,2
Jian Yin1,2, Zhou Zhou1,2, Chuleui Jung3 and Shubao Geng1,2*
...s, scape, pedical and flagellum that consisted numerous individual flagellomeres, respectively. There were eight kinds and twelve types of sensilla on the antennae, including 4 subtypes of sensilla trichodae, 2 subtypes of sensilla basiconica, sensilla chaetica, Böhm bristles, sensilla squamiformia, sensilla auricillica, sensilla coeloconica, and sensilla styloconica. No differences were found about the morphology, abun...

Maysaa A. Jumaah*, Ali A. Al-Iedani 

...xtract of propolis and nigella oil) to current vaccine adjuvants (alum) using inactivated antigens (sonicated and whole-killed bacteria) of E. coli. We also aimed to reveal tthe nature of host innate and adaptive immune responses by measuring the extent of their effects on the level of pro-inflammatory (interferon γ and interleukins (IL)-12) and anti-inflammatory cytokines (IL-4 and IL-10) and innate immune responses (total white blood count, phagocytosi...
Guangcan Lin1,2,3, Xingyu Chen1,2,3, Xiaohao Shi1,2,3, Xiaolin Li1,2,3
Anwar Tanwari Kamran4, Zhengxiang Wang1,2,3, Qing Zhu5, Gaodao Liang5* and Lei Pan1,2,3*
...parva (Temminck and Schlegel, 1842) has a significant potential to spread outside of its current locations and regions, all-continents-spanning invasive range. The invasion of P. parva has threatened the existence of native species. Therefore, evaluation of the condition and fitness of the invasive P. parva population in different regions is necessary. However, no systematic reports of the P. parva length-weight relationships (LWRs) around the world has been d...

Gautam Kumar Deb1*, Md Faizul Hossain Miraz1, SM Jahangir Hossain1, Shahrina Akter1, Md. Ahsanul Kabir1, Md Ruhul Amin2, Md Panir Choudhury1, Nure Hasni Desha1

... stallions (43.65%) over geldings for rearing. A semi-intensive rearing system is identified where farmers provide a combination of roughage and concentrate feed. Bloat, diarrhea, colds, fevers and skin diseases are common among others. Mares experience first heat at 24.02±2.61 months and conceive at 27.09±3.64 months. Foaling length, gestation interval and estrous length were recorded as 26.98±3.14 months, 11.58±0.46 months and 21....

Syeda Rubab Zaidi* and Safdar Ali Mirza

...olumn chromatography and gel filteration method. It has been concluded that locally isolated strain A. niger was one of the potential and promising fungal isolate, screened for laccase enzyme activity, from soil sample collected from agricultural area near Harbans Pura, Lahore. 
 
Novelty Statement | A novel strain of filamentous fungus Aspergillus niger i.e., SAN5 was isolated from soil sample col...
Safaa Sabbar Atiyah1*, Ali Abdullah Zuairi Al-Sadoon2, Shifaa Kadhum Jieish2
...xtract of Aloe vera leaf gel (AEAV) to the epididymal spermatozoa of local goats during the cooling process for different periods. The AEAV was prepared and stored in the refrigerator until the time of use. Epididymal spermatozoa were diluted by a Tris extender and divided into three treatments: T1 with a Tris extender only (control group), T2: 0.02 AEAV, and T3: 0.03 AEAV. Epididymal sperm were cooled at 5 ºC for 0, 24, 48, 72, 96, and 120 hours. The res...

Essa D Alhtheal1*, Sufian S Salman2, Salah M AL-Kubaisi3

...n was prepared using the gelation method. The appropriate cross-linking ratio and cross-linking times of 2% (w/v) chitosan CNPS/cyclosporine nanofibers and solution were prepared. It was dissolved in deionized water at a weight ratio of 2G CNPS diluted with 100 ml DW, and mixtures were prepared by stirring at 80 Co for three and adding five drops of acetic acid. The physical properties and chemical composition were characterized using FE-SEM. Preparation of ch...

Ibrahim Ali1,2, Hanan Mohamed Fathy Abdien1*, Wael Kamel Elfeil1, Mohsen Mohamed Zaky ElDemerdash1, Mohamed Ali Zain El-Abideen2,3, Walid Hamdy Kilany2,3

Ahmed J. Jaafer1*, Alaa K. Jassim1, Nidhal A. Hashim2 

...icipants and placed in a gel tube and left at room temperature for 15 minutes until coagulation, after which it was separated using Centrifuge for 15 minutes, and the serum was stored at a temperature of -20 Celsius until use. Fasting glucose levels were measured using a spectrophotometer, and HBA1c and insulin levels were measured using Cobas e411. Lipid profile, their levels were measured using spectrophotometer. The results showed a significant increase in ...

Nesreen Ahmed Nasser1, Anas H. Sadek2, Bayadir Abdulhussein Mahmeed3, Osama Akram Mohsein4* 

...ticipant and placed in a gel tube. prostate-specific antigen (PSA) and leptin levels were examined using enzyme-linked immunosorbent assays. Result; The results showed that there was no statistical significance in age or body mass index. The results also showed an increase in blood sugar, leptin, PSA, cholesterol, triglycerides, GOT, GPT, urea, and creatinine in the patient group compared to the control group. Conclusions; The increase in all biomarkers in pat...

Bunga Rimta Barus1, Masfria Masfria2*, Aminah Dalimunthe3, Sovia Lenny4

...ate the effectiveness of gel formulations containing the ethyl acetate fraction obtained from red ginger leaves in facilitating the healing process of excision wounds. The study utilized 30 male Wistar rats, divided into six groups with five rats each, to ensure robust statistical analysis. The ethyl acetate fraction was extracted using a detailed maceration and fractionation process with 96% ethanol, followed by formulation into a gel...

Adsadawut Sanannam1, Worawatt Hanthongkul2, Yu-Jing Liao3, Kunlayaphat Wuthijaree4, Pattaraporn Tatsapong4, Amornrat Wanangkarn4,5, Anurak Khieokhajonkhet4,5, Niran Aeksiri4,5, Sureeporn Saengwong6, Wilasinee Inyawilert4,5*

...proteinases (MMPs) using gelatin zymography in pregnant pigs. Eight sexually mature individuals were identified as being in early pregnancy using the vaginal smear technique within 18 to 26 days following artificial insemination. The findings indicated an elevated leukocyte count in pregnant pigs between days 18 and 26. However, a small number of epithelial cells that were either nucleated or cornified were observed during the entire pregnancy period. Addition...

Rael Jepkogei Masai 

...minths are determined largely by climate, which determines mosquito and helminth free-living stage survival. The study was carried out in Nandi Hills Sub-County Hospital in Nandi County and data was collected from 300 pregnant study participants. The study period was stratified into wet season and dry season. Consenting participants provided stool samples that were processed by formal-ether concentration techniques and results recorded as either positive or ne...

Shah Fahad* and Akhtar Ali 

...409 to 1.9682 Kanal averagely. On the basis of problems the study recommend that to make proper law for urbanization in the Country; Provide subsidy and support price to the rural farmer for enhancing agriculture production for high return etc.

...

Wirokartiko Satyawardana1,4, Umi Cahyaningsih2*, Fadjar Satrija2, Safika Safika3, Arifin Budiman Nugraha2

...ncluding Babesia canis vogeli, Hepatozoon canis, Anaplasma platys, and Ehrlichia canis. Using the t-test, data analysis, which covered numerous categories, comprising city of origin, sex, and age for each species of blood parasites and rickettsia, showed that only the male had a significant difference in Babesia canis vogeli infections with P-value of 0.023. In contrast to the other categories, there were no significant diff...

Hamayun Arshad1, Muhammad Waris2,3 and Qurratulann Afza Gardner1*

...tein was subjected to in-gel digestion and peptide mass fingerprinting for identification. Following the successful identification of this protein, we used it to test its toxicity against mosquito larvae by following WHO guidelines with slight modification. The bioassays were performed in triplicate and LC50 was calculated by applying log probit analysis. In conclusion, we found that S-layer protein of L. sphaericus Q001 was effective against the tested larvae...

Eman Wahsh1, Tarek M Ibrahim2, Mirna E. ElAwady3*

...ansmitters. Currently, Vogel conflict paradigm was applied to measure anxiety and validated by using anxiolytic agent, diazepam, and anxiogenic drug, caffeine. Vogel paradigm is based on the conflict between animal’s licking water and receiving electric shocks. Rats were individually tested in the anxiometer for a 10-min session; the licks/shock ratio was fixed at 20 licks/1 shock. In addition to the saline-control gro...
Abrar ul Haq1, Shusheng Yin2, Amara Maryam1, Muhammad Khan1*, Hafiz Abdullah Shakir1, Muhammad Akhtar Ali3, Muhammad Irfan4, Muhammad Faisal Maqbool1 and Yongming Li2*
...y of lathyrol remains largely unknown in various human cancers. The present study was designed to evaluate lathyrol for its broad-spectrum anticancer activity and binding affinity with STAT3 DNA binding domain. Using CCK-8 assay kit, we showed that lathyrol reduced the cell viability of Hep-3B, MHCC97-L, A2780 and taxol resistant Hey-T30 cells in a dose-dependent fashion. Using Molecular docking study, we found that lathyrol exhibits strong binding interaction...

Asfand Yar Khan1, Syed Saleem Ahmad1*, Muhammad Avais1 and Kamran Ashraf2

...ulans @ 25 mg/ml), G4 (Nigela sativa @ 30 mg/ml), and G5 (Calotropis procera @ 10 mg/ml) were taken as treatment groups, while G6 as a negative control, and G7 as a positive control. The results showed that 47.6% serovars were positive for the virulent gene (Stx-1). Moreover, the highest zone of inhibition was observed in G5 (17.1 ± 0.60 mm, P<0.05) as compared to other groups, which indicated high anti-bacterial efficacy on in-vitro basis. The survi...
Roshita Ibrahim1*, Mohd Firza Ahmad Fauzi1 and Mohd Nizam Lani2
...cula-judae) with its gel-like texture in producing healthy drinks, aiming to diversify the market’s range of wholesome beverages. The investigation involves creating a drink with suspended black jelly mushroom pieces and sweetening it with various agents like sucrose, xylitol, erythritol, stevia, and honey. The study evaluates the drinks’ physicochemical, and functional properties, including sensory acceptability to see the potential utilizatio...

Bakht Zada1, Ahmed Zia2, Sardar Azhar Mehmood1, Abdur Rehman3*, Toheed Iqbal4*, Kiran Shahjeer5 and Shabir Ahmed1

...khwa province, remain largely unexplored due to geopolitical challenges, social barriers, and uncertain ground conditions. Despite being covered with dense coniferous forests, District Battagram has never been surveyed for aphids, a prevalent pest in pine plantations globally. This study aimed to investigate the aphid complex affecting pine trees in the district, revealing two speciesCinara atrotibialis and Cinara atlanticathat are newly recorded in the countr...

Sarhad Journal of Agriculture

December

Pakistan J. Zool., Vol. 56, Iss. 6, pp. 2501-3000

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