Lei Chen, Ling Zhu, Zhi-Wen Xu, Wan-Zhu Guo
Molecular Genetics of Aichivirus C (Porcine Kobuvirus) in China
... circulating in China. Recombination events were also observed in Chinese Aichivirus C strains. More further studies are needed to clarify the evolutionary features and pathogenicity of Aichivirus C.
...
Dana Kay Nelkin
...ism about free will on a combination of conceptual and empirical grounds, and the thesis that life on the assumption that we do not have free will can be meaningful and perhaps even better in certain important respects than life on the assumption that we have it. At the same time, there are some subtle shifts in particular commitments, new ways of framing certain issues, and detailed responses to a good number of the very many challenges to the view as origin...
Muhammad Irshad1, Abdur Rab1, Javed Rahman2, Muhammad Sajid1, Ilyas Khan2, Sajad Ali1, Muhammad Razaq1, Sallahuddin1
...- ary in soil media with combination of (silt: Garden soil: FYM at the ratio of 2:1:1) for better growth and development under the agro climatic condition of Swat....
Arshad Ali Khan1, Muhammad Sajid1, Abdur Rab1, Sahib Alam2, Abdul Bari3
...te the effect of various combinations of potassium from organic and inorganic sources on the growth, yield and fruit quality of two tomato cultivars i.e., Falcon and Rio Grande for two years. The response of tomato cultivars was assessed to six combina- tions viz. 0-0 (control), 100-0, 75-25, 50-50, 25-75 and 0-100% of poultry manure (PM) and Sul- phate of Potash (SOP). All the factors (K-sources, cultivars and years) alone ...
Hai-Bo Wang, Qiu-Hua Mo, Ze Yang*
...diagnostic system, which combines nucleic acid sequence-based amplification (NASBA) and molecular beacon technique. Recently there have been two major progresses in the field, in which RT-NASBA technique was applied for the detection of diarrhoea related viruses. This mini review overviewed the basic principle of RT-NASBA and discussed the significance of these new findings....
Imdad Sohu1, Allah Wadhayo Gandahi2*, Ghulam Raza Bhutto2, Muhammad Salim Sarki2, Rabail Gandahi3
...re and poultry manure in combination with inorganic NPK fertilizers had shown positive effect on chickpea plant height, number of branches plant-1, number of pods plant-1 and seed index. The growth and yield of chickpea significantly increased with the collective application of chemical fertilizers and organic manures. It is concluded that the application of half of recommended dose of NKP (18-36-10 kg ha-1) in combination ...
Muhammad Nawaz Kandhro*, Habib-Ur-Rehman Memon, Muhammad Ali Ansari, Ahmed Naqi Shah
...to weedy check. The combined application of sorghum @ 15 L ha-1+ Dual Gold @ 1.25 L ha-1 resulted in weeds mortality upto 66.6%, produced seedcotton yield of 3961.4 kg ha-1 and gave net benefit of Rs. 2,20,029. Sunflower applied @ 15 L ha-1 in integration with Dual Gold @ 1.25 L ha-1 showed weeds mortality of 65.5 %, resulted in seedcotton yield of 3949.7 kg ha-1 and net monetary benefit of Rs. 2,19,821. Sole application of Dual Gold @...
Temitope Oluwasegun Cephas Faleye1, Johnson Adekunle Adeniji1, 2 *
... and E19 (3 isolates). Recombination analysis showed evidence of recombination events. Results of this study showed that the NPEV- Cs circulating in south-western Nigeria also contributed to the emergence of cVDPV2 previously described between 2005 and 2011. Hence, the risk of recombination between OPV and NPEV-C members to generate recombinant VDPVs in ...
Mohammed A. Rohaim1*, Rania F. El-Naggar2, Abdulrahman M. Gamal3, Elshaimaa Ismael3, Mohamed M. Hamoud4, Sherif T. Moubarak3, Ashraf M. Metwally1, Manal M. Zaki3, Shimaa A.E. Nasr3, Samah Elsaid3, Mohamed M. Ali3, Hussein A. Hussein1 and Osama K. Zahran3
Email: mohammedvet1986@gmail.com; mohammed_abdelmohsen@cu.edu.eg
...nes were devised to combat the spread of highly pathogenic avian influenza (HPAIV) H5N1. HPAIV are highly susceptible to all disinfectants because they are enveloped viruses. Disinfection against avian influenza viruses at the poultry farms would significantly reduce and/or limit the chance for its transmission and outbreaks. Many disinfectants have been evaluated for their inactivation ability, but there is still a need for their evaluatio...
Muhammad Imran1*, Muhammad Mobashar2, Muhammad Irfan3, Shazia Hanif4, Sumaira Hanif5 and Muhammad Abubakar6
...tely with MOLM however a combination of MOLM and CSC improved milk yield than either protein source fed alone in lactating Nili-Ravi buffaloes in Pakistan....
Tauseef Taj Kiani1*, Mozammil Hussain1 and Hidayat ur Rahman2
...ten F1 hybrid combinations, which were used to develop three generations F2, BC1 and BC2. Heterosis (in F1) and inbreeding depres- sion (in F2, BC1 and BC2) were quanti ed for traits including shelling percentage, kernels per row, kernel rows per ear, and 100-grain weight and grain yield. Non-additive gene action was evident for shelling percentage, kernels per row and 100-...
Mohammad Taghi Karimi
...deformities were used in combination with orthosis, brace and corset. The searched outcome indicated that there were at least 15 articles published on this topic and most of them have a small number of subjects. Although some new orthoses have been developed to decrease the curve of kyphosis, most of them did not test on more number of subjects. It seems that Milwaukee orthosis carry higher effciency to reduce the curve of kyphosis. There are not enough eviden...
Muhammad Amjad Nadim1*, Mohammad Safdar Baloch1, Ejaz Ahmad Khan1, Abdul Aziz Khakwani1, Kashif Waseem2
..., Cu and B (alone and in combinations) were assigned to sub-plots. The results revealed significant variations in plant growth and yield attributes with recommended doses of NPK fertilizers. This treatment (NPK at recommended levels) resulted in higher leaf area index (0.33 and 0.27 [49 days after sowing-DAS] and 2.03 and 2.77 [98-DAS]), crop growth rate (40.42 and 40.96 g m-2 day-1), relative growth rate (88.94 and 84.14 mg g-1 day-1), net assimilation rate (...
John G. Bruno1*, Chien-Chung Chao2,3, Zhiwen Zhang3, Wei-Mei Ching2,3, Taylor Phillips1, Allison Edge1, Jeffery C. Sivils1
...ure and reporter aptamer combinations and tested in a fluorescent peroxidase-linked Amplex® UltraRed (AUR; a resazurin-like substrate) version of a rapid (< 1 h) aptamer magnetic bead sandwich assay. The optimal sandwich aptamer combination utilized the same aptamer sequence (designated Rt-18R) for both capture and reporter functions while producing a signal to noise ratio of > 4.0 for detection of ~ 1,000, R. t...
Shahida Bibi* and Ijaz Ahmad Khan
...ied under different crop combination at the Research Farm of The University of Agriculture Peshawar, Pakistan during the year 2012. The study was carried out in a split-plot design with three replications. Herbicide treatments (herbicide used and herbicide not used) were assigned to main plots, while intercropping treatments (sole maize, sole mungbean, 5 rows of mungbean + 6 rows of maize, 10 row of mungbean + 6 rows of maize) were allotted to subplots. Result...
Tahir Khan, Raziuddin, Fakharuddin* and Muazam Jamal Razi
.../sub> populations, cross combinations CA5 × DH8, CA2 × DH7, CA2 × DH8, CA5 × DH7, CA4 × DH7 and CA4 × DH8 performed better. Plant height and main raceme length displayed moderate to high broad sense heritability and maximum genetic advance for most of the F4 populations. Current results suggested the effectiveness of selection in early generations for the improvement of these traits. ...
Qaizar Ahmed1, Fida Mohammad2, Hidayat-ur-Rahman2, Sheraz Ahmed2* and Fakharuddin2
... crossed in all possible combinations to generate 7 x 7 diallel crosses. In 2008 and 2009, all F1 hybrids along with their parent cultivars were planted in randomized complete block (RCB) design with four replicates at Tobacco Research Station Mardan (plain) and Tobacco Research Substation Mansehra (hilly). Data for all traits were analysed in pooled analysis and individual environments. Heterosis over better parent was determined for all traits acr...
Amitha Reena Gomes1, Belamaranahally Muniveerappa Veeregowda2, Sonnahallipura Munivenkatappa Byregowda1, Vinayagamurthy Balamurugan3*
...ls. With the advent of recombinant DNA technology, recombinant protein based vaccines and/or diagnostics are being tested in various heterologous systems across the globe for development of vaccines and/or diagnostic antigens. The recombinant viral proteins, virus like particle based vaccines, bivalent/multivalent vaccines, recombinant viral vectored vac...
Zar Muhammad and Mohammad Tariq Jan
...ed as sole or in various combinations six weeks before sowing and then were incorporated to plots at sowing both the years. All treatments including control were either supplemented with half of the recommended dose (75 kg N ha-1) of Urea-N or no Urea-N. Compost, its composition, mineral N and their interactions significantly affected maize yield and yield components over two years average data. Compost application produced more maize ears m-2 (13%), grains ea...
Muhammad Ishaq and Raziuddin
...ceme length. Analysis of combining ability revealed highly significant GCA (general combining ability), SCA (specific combining ability) and RCA (reciprocal combining ability) effects for the studied traits. Based on GCA, best general combiners were AUP-7 for earlier flowering and maturity, AUP-2 for plant height, AUP-...
Farmanullah Khan, Samad Khan, Wiqar Ahmad and Imran Khan
...ands during 2007-08. The combination of nutrient sources tested were; control, NPK50 (50% NPK), NPK75 (75% NPK), NPK100 (100% NPK), FYM10 (10 t ha-1 FYM), NPK50+FYM10, NPK75+FYM10, NPK100+FYM10, FYM20 (20 t ha-1 FYM), NPK50+FYM20, NPK75+FYM20, NPK100+FYM20. Results revealed that NPK75FYM20` significantly (p<0.05) improved the yield. As regards soil properties, NPK20FYM20 reduced bulk density, increased WHC, saturation percentage, organic matter content, pla...
Christopher U Orji1*, Ignatius O Onyeocha2, Steven S Shaida3, Peter M Dede4, Bitrus Yakubu5, Elijah E Ella6 and Pam D Luka5
...he two species. Based on combined loci of cytochrome oxidase II (COII) and cytochrome b (CytB), twelve haplotypes were generated for G. p. palpalis and five for G. m. submorsitans. Similarly, for the combined loci, haplotype diversity (Hd) was higher for G. p. palpalis (0.90) than G. m. submorsitans (0.68). Nucleotide diversity was homogenous for G. m. submorsitans (0.24859) than G. p. p...
Muhammad Ibrahim, Jawaria Ali Khan, Muhammad Sarwar Khan, Wasim Shehzad, Muhammad Avais, Ali Husnain, Naimatullah Khan, Muhammad Kamran Ameen and Abdullah Iqbal
...arlic and Vitamin E + Se combination in cows....
Allah Wadhayo Gandahi, Khalilluah Panhwar, Rabail Gandahi, Muhammad Saleem Sarki and Mahmooda Buriro
...plied @ 120-70-40 NPK in combination with Zn and B @ 4 and 1.5 kg ha-1, respectively....
Laila M. Fadda1, Nouf M. Al-Rasheed1, Iman H. Hasan1, Hanaa M. Ali2,3*, Nawal M. Al-Rasheed1,4, Musaed Al-Fayez5, Aly M. Ahmed5, Nada Almutlaq1, Nehal Qasem1 and Reem Khalaf1
...(CUR) either alone or in combination on liver injury induced by inflammation, oxidative stress in response to APAP toxicity in rats. Serum aminotransferases (ALT and AST), alkaline phosphatase (ALP), lactate dehydrogenase (LDH), total protein, total bilirubin, hepatic glutathione (GSH), nitric oxide (NO), superoxide dismutase (SOD) and lipid peroxides (LP) levels were estimated. Moreover these biochemical parameters were confirmed by histopathological examinat...
Aqila Shaheen and Mehwish Matien
...ltivated land uses. Data combined over locations illustrated that Mollisols contained higher SOC and K, whereas, TN, AP, and BD were statistically similar at both sites. The SOC was positively correlated with TN, AP and K of soil, whereas, SOC was negatively correlated with BD and pH of the soil. There is a large difference in SOC storage and physico-chemical properties mainly in relation to climate, soil type and vegetation....
Pan-pan Guo1, Wei Liu2, Yan Li2, Rui-yu Ma2, Wang Zaigui1*, Kai Zhan2*, Jun-ying Li2 and Sheng-nan Liu2
...hase program. Haplotypes combinations constructed on these two SNPs of HTT gene were associated with some reproduction traits. In particular, diplotype H1H1 had positive effect on reproduction traits, diplotype H2H4 had negative effect of reproduction traits. In conclusion, our study showed that polymorphisms of the HTT gene were significantly associated with egg production traits in two Anhui local chicken breeds, but the suggestions that HTT may be considere...
Taghreed A. Hafiz1, Murad A. Mubaraki1*, Mohamed A. Dkhil2,3 and Saleh Al-Quraishy2
... annuum could be used in combination with standard drug to treat herpes infection....
Süleyman Kök1*, Sertaç Atalay1, Hasan Semih Eken2 and Mustafa Savaşçi3
...nd all of their genotype combinations, were also found to be carried by TGC....
Wenmin Cheng1,2*, Weirong Pan1,2, Yubo Qing1, Yingchao Liu1, Xingqin Zha1,2, Yan Huang2, Jige Xin1,2, Hongjiang Wei1 and Yangzhi Zeng2
...(BMI), we obtained the recombinant nucleoplasmin (Npm) by using a prokaryotic expression system and compared the difference on the developmental effects between exogenous Npm and its structural analog, polyglutamic acid (PGA). We chose the pMD18-T vector for Npm expression and transformed E. coli BL21 (DE3) cells to construct a prokaryotic expression system. The results showed that the recombinant Npm protein of 32 kDa could...
Muhammad Sohail1,*, Muhammad Naeem Khan1, Naureen Aziz Qureshi2 and Abdul Shakoor Chaudhry3
...copper (Cu) alone and in combinations (Pb + Cr + Cu) for 15 days under laboratory conditions. Gill cells of mussels were used to determine the DNA damage by comet assay. The tail DNA (%), comet tail length and olive tail moment (OTM) were the parameters selected to detect DNA damage. Low doses (120 µg L-1) of each metal induced significantly higher levels of DNA strands breaks as compared to medium dose (240 µg L-1) and very low levels of DNA damag...
Muhammad Hafeez-ur-Rehman1, Farzana Abbas1, Muhammad Ashraf1, Naeem Tariq Narejo2, Khalid Javed Iqbal3, Ghulam Abbas4* and Syedah Andleeb5
...treatment 2 (35% CP), in combination of ovaprim + fresh PG showed very short latency period when compared to the ovaprim+HCG injected fish. Treatment 1 and control failed to spawn. Fish fed on 30% protein diet (treatment 1) and the control had the lowest fecundity showing insufficiency of protein required for proper development of the ovary. These studies revealed that 40% protein diet not only improved fish growth and health but also enhanced egg fecundity, g...
Muhammad Saeed* and Iftikhar Hussain Khalil
... 3 replications. General combining ability (GCA), specific combining ability (SCA) and narrow sense heritability for maturity and yield components traits were determined. The main objective of the research was identification and proper selection of best performing wheat parental genotypes and best F1 crosses, based on GCA and SCA estimates. Significant differences were observed among the wheat genotypes for all the studied t...
Salma Shaheen, Mumtaz Khan, Muhammad Jamil Khan, Saleem Jilani, Zarina Bibi, Muhammad Munir and Mehwish Kiran
...howed that EM + pressmud combination significantly improved soil physical and chemical properties as well as spinach growth compared to other fertilizers with EM. Maximum soil organic matter content (OM) and K was observed in treatment where pressmud was co-applied with EM. While greater N and P were found where compost and poultry manure were co-applied with EM respectively. Significant (P < 0.05) increase in plant height (11%), number of leaves (124%), fr...
Hina Fatima, Lal Almas and Bushra Yasmin
...ng cotton-melon crops in combination under tunnels. The study area was Punjab, Pakistan. The stochastic cost frontier analysis was used to estimate the allocative efficiency of cotton-melon cropping systems efficiency. The result illustrates that none of the farmers to be optimally using their inputs. The average allocative efficiency was around 75%. Approximately 36 percent of farms allocative efficiency varies from 0.20 to 0.60. Around 60% of farms allocativ...
Sana Irshad Khan1*, Zafar Iqbal2, Hamid Ullah Shah2 and Shaukat Hussain3
... of novel antibiotics to combat resistant pathogens....
Ibni Amin Khalil* and Raziuddin
...was further subjected to combining ability analysis. For the inheritance of the studied trait (seed yield plant-1), additive as well as non-additive genetic mechanisms were found important due to significant effects for both components i.e. General Combining Ability (GCA) and Specific Combining Ability (SCA) effects. Maximum GCA was shown by L-7 and T-1; whereas high SCA was noted for L-6 ...
Yusuf Koc1, Ecevit Eyduran1* and Omer Akbulut2
...ee methods for different combinations of parent node: child node in the data set regarding animal science. To achieve the aim, 1884 Mengali lambs were provided for predicting weaning weight from sex (male and female), birth type (single and twin), birth year (2005, 2006, 2007, 2008 and 2009), farm (Research station, Mastung, Quetta, and Noshki), birth weight, dam age, and dam weight. To choice the best regression tree method, goodness of fit criteria such as c...
Lingtong Ye1, Chao Cao1,2, Bin Tang1,2, Tuo Yao1, Ruixuan Wang1 and Jiangyong Wang1*
... taxonomic approach that combines morphology and molecular analysis of the mitochondrial cytochrome c oxidase subunit 1 (COI) gene. Adult P. websteri exhibit a high degree of morphological plasticity in the palp pigmentation pattern, the shape of the anterior edge of the prostomium, the shape of the major spines on chaetiger 5, and the shape of the pygidium. The COI gene sequence demonstrated that the intraspecific distance of P. websteri was 0.3...
Pam D Luka, Frank N Mwiine, Bitrus Yakubu, Joseph Erume, Ricardo Pérez-Sánchez, Hermann Unger and David Shamaki
...k antibodies using the recombinant TSGP1 indirect ELISA. Of the samples analysed, 13.4% (442/3,288) showed moderate to high reactivity, suggestive of domestic pigs’ exposure to tick-bite. Antibody reactivity’s were found in eight of the 10 states studied and all the states have favourable climatic conditions for soft ticks’ survival and national parks harbouring wild swine (warthogs) able to maintain the developmental cycle of these ticks. Th...
Md. Yeamin Hossain1,*, Md. Alomgir Hossen1, Md. Nasir Uddin Pramanik1, Fairuz Nawer1, Md. Mosaddequr Rahman2, Suraiya Sharmin1, Dalia Khatun1, A. H. Bahkali3, Abdallah M. Elgorban3 and Khairun Yahya4
...wth in male, female, and combined sexes (b < 3.00, p < 0.001). All LLRs were highly significant (p < 0.001), with coefficients of determination (r2) values exceeded 0.98. Among the condition factors, KF is the best index for assessing the well-being of this species in the Ganges River. The WR was not significantly different from 100 for males (p = 0.298) and females (...
Glomus mosseae (Gerd and Trappe) and Neemex Reduce Invasion and Development of Meloidogyne incognita
Atta ur Rehman Khan1,2*, Nazir Javed2, Shahbaz Talib Sahi2, Tariq Mukhtar1, Sajid Aleem Khan2 and Waqas Ashraf3
... each harvest, neemex in combination with G. mosseae proved the most effective as the development of nematode was adversely affected. Developing juveniles and adults were less in number in the combined treatment.
...
Sadaf Ijaz Malik1, Kiran Afshan2* and Mazhar Qayyum1
...phistome adults and eggs combined with examining pathological, hematological and bile biochemical response in buffaloes to Gigantocotyle explanatum infection. Of the 558 slaughtered buffaloes from central Punjab, Pakistan, 18.99% were infected, and morphometric analyses were made of 115 mature amphistomes and gravid eggs with traditional microscopic methods on the basis of standardized measurements. Amphistomes were flattened and stained in Borax carmin...
K.N. ArulJothi1, M. Abinaya1, B. Suruthi Abirami1, Melvin George2, S. Elangovan3 and A. Devi1*
...sms was done by PCR-RFLP combined with gel electrophoresis. The genotype distribution in the population was found to be consistent with Hardy Weinberg equilibrium. Through logistic regression analysis, it was observed that, in Tamil population the CC and CT genotype carriers have higher odds of developing myocardial infarction (OR – 1.64 and 2.04 respectively) and for developing hypercholesterolemia (OR – 2.02 and 2.13 respectively). In the t...
Nosheen Noor Elahi* and Misbah Mughal
...ious stem epidermal cell combinations and structures like number of stomata, number and shape of cork, silica, long, and pointed elongated cork cells. It is therefore contended that the structure of the epidermis in sugar cane can be used as a characteristic of variety differentiation....
Sheraz Ahmed and Fida Mohammad
... program. One hundred recombinant inbred lines (RILs) derived from four F4 populations of Flue Cured Virginia (FCV) tobacco were tested to identify effective selection indices. Experimental material was planted at two locations i.e. Mardan (E-1, E-3 and E-5) and Mansehra (E-2, E-4 and E-6) using alpha lattice design with three replicates during 2012/13, 2013/14 and 2014/15. Heritability in broad sense was generally low for all traits except nicotine and reduc...
Erum Bughio1, Ahmed Sultan Jatoi*1, Muzamil Memon2, Reema Bughio3, Pervez Ahmed Khoso4, Zafar Ali Khoso1 and Ali Asghar Baloch5
... vaccinated with various combinations of IBD through different routes. Results showed that the birds of different groups received different maternal antibodies did not induce any antibodies as recorded in all groups received vaccines at days 1, 9 or 12 exhibited similar pattern antibody profiles, however, an increase in the mean titer of antibodies was recorded from 17 and onwards. Furthermore, on day 39 of age, the birds of all groups including control were c...
Habib Ullah Khan1*, Saleem Ullah1, Hamid Ullah Shah1, Muhammad Arif2
...s C, F, N,S, P and their combination i.e.CF, CN, CS, CP, FN, FS, FP, NS, NP, SP, CFN, CFS, CFP, FNS, FNP, FSP, CNS, CNP, CSP, FSP, NSP, CFNS, CFNP, CFSP, CNSP, FNSP, CFNSP. Quality of the treated wood coal and their effect on soil pH, Electrical Conductivity (EC), acidity, total nitrogen and other minerals was studied. pH and EC of the treated wood coal ranged from 1.48 to 11.9 and 3.45 to 28.4µs/dl respectively. Soil EC was lower 96.60 µS/dl in un...
Wesam Hasan Mohamed Mady 1*, Bing Liu2, Dong Huang2, A. Arafa1, M.K. Hassan1, M.M. Aly1, Pucheng Chen2, Yongping Jiang2* and Hualan Chen2
...in vitro expression of recombinant plasmid DNA was performed by transfection of 293T (HEK) cell line. The HA protein was analyzed using SDS-PAGE followed by Western blot and immunofluorescence assays. The pCAGG-optiH9 vaccine efficacy was evaluated by intramuscular immunization of SPF chickens with different concentrations of plasmid DNA and the sera were collected weekly post vaccination for antibody detection by HI test. All immunized chickens shown high HI ...
Maoying Zhu, Yuntao Ji, Xiaoyu Wang, Junying Pu and Changqing Qu*
...em cells. Four different combinations of cryoprotectants with and without hydrogen gas (H) (purified H2 was dissolved into normal cryopreservation solution for 2 hrs under 0.6 MPa were tested including the following groups: control (CK), 100µm GSH, 10µm fasudil hydrochloride (FH) and a combination of the two (GSH + FH). A solution of 10% (v/v) Me2SO + 20% FBS was used as the standard cryopre...
Response of Spring and Autumn Sunflower to Chemical and Bio-Fertilizers for Yield and Quality Traits
Muhammad Tahirand Memoona Shehzadi*
...atments comprised on the combination of chemical fertilizers (No chemical fertilizer, half recommended dose of fertilizer and full recommended dose of chemical fertilizer) and Plant Growth Promoting Rhizobacteria (PGPR) (No PGPR, PGPR-1, PGPR-2 and PGPR-1 + PGPR-2). The PGPR used in this study were pre-isolated and characterized, taken from soil microbiology and biochemistry laboratory, Institute of Soil and Environmental Sciences (ISES), University of Agricul...
Abdus-Subhan1, Qudratullah Khan1, Muhammad Mansoor2, Muhammad Jamil Khan1, Ammanullah2, Matiullah Khan3
...as recorded in treatment combination of P2 × S1.It is concluded that use of 2-tillers on raise bed sowing method gave significant result of WUE, economics and grain yield which may be recommended for the arid regions.
... 
Nirbhay Kushwaha, Achuit K Singh, Brotati Chattopadhyay and Supriya Chakraborty
Recent advances in geminivirus detection and future perspectives
... to detect individual or combinations of viruses with sensitivity comparable to ELISA. Methods based on PCR provide the greatest sensitivity among the listed techniques but are limited in parallel detection capability even in “multiplexed” applications. Better, easier and cheaper than polymerase chain reaction or antibody detection, rolling circle amplification using the bacteriophage 29 DNApolymerase allows for a reliable diagnosis of geminiviruse...
Sitansu Pan and Amrita Das
Evaluation of shelf life of some value added organic formulations of Trichoderma harzianum
...an and FYMand also their combinations with two oil cakes, neem and mustard cake. The growth was estimated in terms of colony forming unit (c.f.u.)/g of substrate up to 120 days at 30 days interval. In all organic products or their amendment with oil cakes c.f.u. increased up to 60 days and then gradually declined. Among the four organic media, vermicompost retained 21.3 x 10 c.f.u./g at 120 days where as leaf mold,FYMand rice bran retained 20.1 x 10 c.f.u./g, ...
S. K. Mukhopadhyay and M. V. Santha Kumar
Studies on the biosafety of botanical insecticides to native natural enemies of mulberry ecosystem
...tract in different conc./combinations along with dimethoate and dichlorvos on four coccinellid bio-control agents (Micraspis crocea, Micraspis discolor, Brumus suturalis and Scymnus bourdilloni ). All botanicals tested showed least mortality and was on par with unsprayed control. Dimethoate (0.1%) was highly toxic causing 100% mortality. Whereas, dichlorvos (0.1%) was least toxic in comparison to dimethoate. Botanical insecticides tested in different conc./
1Parvin Noor and 2Md. Nashir Uddin
Morphological changes of different castes of the subterranean termites (Odontotermes proformosanus) in fungus combs of termitophiles
...enomena of making fungus combs inside the termitophiles. Considering the consistency of fungus-bed in the termitophiles, an attempt has been made to observe the morphological changes of nymphs while they stayed within the fungus comb. Biological attributes indicated that Odontotermes proformosanus are polymorphic in nature and have exopterygote post-metamorphic development. During the period of study, eggs and nymphs were co...
Sitansu Pan and Amrita Das
Control of cowpea (Vigna sinensis) root and collar rot (Rhizoctonia solani) with some organic formulations of Trichoderma harzianum under field condition
...ong with other treatment combinations. It was observed that seed priming with mycelia preparation of the antagonist Trichoderma harzianum at 4g/kg of seed and organic formulation of the antagonist in vermicompost along with 20% neem cake (w/w) gave best disease control along with yield of cowpeas over leaf mold +20% neem cake formulation of the antagonist, talc base formulation and seed treatment with carbendazim at 2g/kg of seed. Seed priming with mycelial ma...
Biswajit Pramanick, Sruti Karmakar, Koushik Brahmachari, Rupayan Deb
An integration of weed management practices in potato un-der New Alluvial soil
...rbicides and mulching in combination to cope up with the labour crisis and minimize the cost of cultivation, therefore, maximizing benefit cost ratio....
K. K. Hegde and B. S. Nandihalli
Bioefficacy of some indigenous products in the management of okra fruit borers
...ried individually and in combinations along with endosulfan (0.07%) and untreated control. Repeated sprays of GCKE recorded least number of eggs (1.40 eggs/plant) followed by NSKE (5%) alternated with cow dung 10 per cent (1.71 eggs/plant). Repeated sprays of cow dung and cow urine used individually were not effective against the borers and recorded higher fruit damage of 55.70 and 52.10 per cent, respectively. GCKE was significantly superior in reducing the f...
Azhar Abbas Khan1,*, Arif Muhammad Khan2 and Muhammad Afzal3
...s were used singly or in combination with prey as a host. An increased numbers of C. septempunctata was observed when host plants were added with aphids. But was no significant difference in most of the combinations except wheat with aphids (3.8889±0.4554). The fresh leaves of rose, citrus, brassica and wheat (1.2±0.2006, 1.3556±0.1416, 1.5778±0.1829, 2.1444±0.2027, respectively) att...
Muhammad Imran Shabbir
...to describe the possible combinations of paternal and maternal alleles for a particular cross. Described here is a line-dot method using ternary genotype codes to determine genotype probabilities in a very quick and simple way. Also ternary genotype codes along with gender symbols and affection status are used to modify the Punnett squares. On applying these modified Punnett squares along polygonal shapes covers all possible parental c...
Adnan Yousaf1,Jia Wu1, Qaiser Shakeel2, Yasir Iftikhar3, Muhammad Irfan Ullah4, Uzma Tahira5,Mustansar Mubeen1 and Wubei Dong1,*
...ii separately and in combination as well. At harvesting, roots of C-302 contained significantly fewer galls (30) and egg-masses (51) compared to the other fourteen cultivars. Seven cultivars including C-33, Gola Peshawari, 11-2010, 18-2010, 15-2010, 27-2010 and C-68 had 10 root galling and egg-masses indices (from a scale of 0-10). Hence, present study shows that none of the available chili cultivars are resistant to attack from M. incognita and ...
Wesam Hasan Mohamed Mady1*, Bing Liu2, Dong Huang2, Abdel Satar Arafa1, Mohamed Khalifa Hassan1, Mona Mehrez Aly1, Pucheng Chen2, Yongping Jiang2* and Hualan Chen2
...st antigens encoded by recombinant DNA. The goal of this study was to construct Egy-H5 plasmid DNA-based vaccine against a currently circulating avian influenza virus H5N1 strain in Egypt targeting the HA gene. The HA gene of AIV Egyptian strain H5N1 was codon optimized for chicken biased codon and sub-cloned into pCAGGS mammalian expression vector under the control of chicken β-actin promoter. The construct was transfected into 293T HEK (Human Embryonic ...
Yijin He1, Song Ma2, Bo Liu1,*, Ting Xue1, Qunlan Zhou1, Wu Jin1 and Kui Chen2,*
...n spectrum of tissues in combination with bioinformatics analysis so as to understand the possible functions in response to chemical sensation behavior of taste receptors. The receptors when compared with T. rubripes, there was 99.2% identity in two kinds ORF sequences, with only 7 bases different, and there were 5 amino acids different between speculated fusion proteins. Under bioinformatics analysis, the T2R1 gene without introns was consistent...
Murad Ali1, Farmanullah Khan1, Wiqar Ahmad2* and Imran Khan1
... beneficially and easily combine to give maximum output still needs lots of research. The experiment was conducted during wheat season 2014-15 on a selected water eroded site in District Swabi. Fourteen treatments viz the control (T1), recommended dose (RD) (120:90:60 kg ha-1 N: P2O5: K2O. T2), four combinations of farmyard manure (FYM) i.e 20, 15, 10 and 5 t ha-1 with 0, 25, 50, 75% of the RD (T3, T4, T5, T6, respectively),...
Sumaira Maqsood1,*, Muhammad Afzal1, Muhammad Anjum Aqueel1, Abu Bakar Muhammad Raza1,Waqas Wakil2 andMuhammad Hussnain Babar3
... (69.35±1.66%) in combined application of NPV and flubendiamide. Dose of flubendamide was the key factor for type of interaction, its combination with NPV at lower dose showed synergistic interaction (CTF≥20). While rest of the combination showed additive effect (CTF≤20). Integration of NPV and flubendiamide proved more fatal at higher concentrations than the individual applic...
Muhammad Arslan Khan1,*, Sajid Aleem Khan1, Imran-ul-haq1 and Rashad Waseem Khan2
...i> (Rifai) (alone and in combination) were used as bio-control agents against isolated pathogens at three different concentration viz., R, R/2 and R/4. Combination of bio agents (T+P) gave maximum results at R concentration against R. bataticola and M. incognita.
...
Tanveer Ahmad1, Hafiz Sultan Mahmood1*, Zulfiqar Ali1, Muhammad Azeem Khan2 and Shahid Zia3
...rticator together with a combing machine was Rs. 507.9 h-1. The break-even point or payback period of this technology was 233 h. Sisal fibre needs to be washed, dried and brushed after decortication process for getting good quality fibre to make domestic products. This technology has a great potential and needs to be commercialised in sisal growing areas of the country.
...
Mohammad Umar Farooq1*, Sarfaraz Ahmad2, Ghulam Nabi1, Ijaz Ali1 and Imtiaz Ahmad1
Mian Ahmad Raza*, Ghulam Hassan and Naqib Ullah Khan
...performed to investigate combining ability effects and gene actions for some important traits using diallel mating design in wheat at Peshawar Pakistan. Eight wheat genotypes viz. Janbaz (JNB), Fakhr-i-Sarhad (FS), Pirsabak 2005 (PS), AUP-5008 (AUP), Saleem 2000 (S2K), Tatara (TTR), Barsat (BST) and Siren (SRN) were crossed in all possible combinations during 2011-12 cropping season. The resultant F1 hybrids were planted fol...
Furqan Sabir1, Muhammad Tayyab1,*, Bushra Muneer2, Abu Saeed Hashmi1, Ali Raza Awan1, Naeem Rashid3, Muhammad Wasim1 and Sehrish Firyal1
...nd characterization of recombinant thermostable phytase from Thermotoga naphthophila (PHYTN). This study was an initial step for the fulfilment of domestic industrial requirement of phytase in Pakistan.The PCR resulted in the amplification of 1.8 kb phytase gene. SDS-PAGE confirmed the size of recombinant protein as 70 kDa. The optimization studies demonstrated the maximal production of re
Abdus Subhan1, Qudrat Ullah Khan1, Muhammad Mansoor2 and M. Jamil Khan1
...ective of their kind and combinations, exerted significant (p≤0.05) variation in plant growth, yield parameters of wheat and water use efficiency. The inorganic fertilizer gave significantly higher total dry matter, grain and straw yield and also due to the greater grain yield the water use efficiency calculated was greater in the NPK treatment. Bulk density, porosity and organic matter were significantly improved by the cattle manure and compost treatments...
Zaheer Abbas*, Shaukat Ali, Jalal-Ud-Din and Ghulam M. Ali*
... only single auxin while combination of auxin and cytokine in MS and Chu’s N6 media produced 53.33 and 35.56% embryogenic calli, respectively. Inclusion of 5 mgL-1 silver nitrate in embryogenic medium enhanced the production of type II embryogenic calli by 54.90% compared to control treatment. Maximum regeneration (58.33%) was observed when embryogenic calli were placed on N6 medium supplemented with 60 gL-1 sucrose and 1 mgL-1 NAA for one week initially...
Asmatullah Kaka2, Wahid Haron1,*, Rosnina Yusoff 1, Nurhusien Yimer1, A.M Khumran1, Akeel Ahmed Memon2, Kazhal Sarsaifi1 andMahdi Ebrahimi3
...ty acids. In conclusion, combination of ALA and DHA did not improve frozen-thawed bull sperm parameters when compared to control.
...
Shengyan Su, Zaijie Dong*, Wenbin Zhu, Lanmei Wang and Jianjun Fu
...mes;3 full diallel cross combinations used for selection was built to investigate compensatory like growth of each combination. Yes/No(Y/N) effect was determined for specific combination dyads, which there were significant differences in body weight at tagging, but no difference in body weight at harvesting. Among the 36 dyads, 7 had an YN response (19.4%). When the YN effect had a fixed a...
Faiz Muhammad1,*, Syed Khurram Fareed1, Urooj Zafar1, Taseer Ahmed Khan2 and Aqeel Ahmad1
...oth culture and PCR were combined as culture-enhanced PCR approach. Three different DNA crude preparation methods were used and broth dilution method found simpler and most efficient for positive PCR. Primers were selected for 16s rRNA and it could detect up to 100 cfu and 250 cfu from MS and MG respectively in a samples. We have tested pure DNA of other mycoplasmas (5 species from avian origin and 3 other mycoplasmas species) but it produced only the genus sp...
Zhenyu Chang1, Hui Zhang1, Khalid Mehmood1,2, Mujeeb Ur Rehman1, Xiaodan Yuan1, Zhaoyang Li1, Fazul Nabi1, Xiaoxing Wu1, Xinxin Tian1, Xueting Liu1, Jingen Xu3 and Donghai Zhou1,*
...lysaccharide (LPS) and recombinant human (rh) TFF3. Cell proliferation and TFF3 mRNA expression were examined by 3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide (MTT) assays and real-time quantitative PCR, respectively. The results showed that rhTFF3 effectively prevented the cell injury induced by LPS (P<0.05). A low concentration of LPS (10 µg/mL) down-regulated the expression of TFF3 mRNA, whereas a high concentration of LPS (...
Bibi Haleema1*, Abdur Rab1 and Syed Asghar Hussain2
... can be used alone or in combination to improve growth and fruit production of tomato. ...
Wen-Feng Li, Xiao-Yan Wang, Ying-Kun Huang*, Rong-Yue Zhang, Jiong Yin, Zhi-Ming Luoand Hong-Li Shan
...ugar yield caused by the combined occurrence of Tetramoera schistaceana (Snellen) and Chilo sacchariphagus (Bojer) were measured in pesticide–treated and untreated areas during 2014-2016. The results indicated that the in untreated areas, percent dead-heart was 8.30% - 58.83%, the percent stalk injured was 26.73% - 96.67%, the percent internodes bored was 4.40% - 25.87%, and the effective stem number was reduced by 4440-21900 stalk/h...
Rana Fartab Shoukat1, Shoaib Freed1,*, Kanwar Waqas Ahmad1 and Ateeq-ur-Rehman2
...ith insecticides and the combined application can improve the management program of C. pipiens.
...
Safdar Ali1*, Ghulam Abbas Shah1, Muhammad Naveed Tahir1, Shahid Mehmood2, Asim Gulzar3 , Ijaz Ahmad4 and Bashir Ahmad Khan5
...different tillage systemscombined with glyphosate herbicide on soil moisture content,bulk density and porosity associated with wheat grain yield were evaluated. Seven tillage treatments were applied in the fallow periods (summer seasons) of both years before sowing of wheat crop. These tillage treatments were (i) Once Mould board plowing followed by 8 cultivations with planker (1 MBP + 8 CS) as a check, (ii) No-till followed by twice application of glyphosate ...
Malak Atiq Ullah Khan* and Fida Mohammad
Faiza Hassan1, Mubshara Saadia1,*, Muhammad Sher1, Mian Anjum Murtaza2, Muhammad Arshad3,Asam Riaz4 and Mahmood Ahmad Khan5
... be applied in herb-drug combinations commonly used as antidiabetic.
...
Muhammad Usman Saleem1, Saima Masood1,*, Hafsa Zaneb1, Aneela Zameer Durrani2, Asim Aslam3, Kamran Ashraf4, Habib-ur-Rehman5, Muti-ur-Rehman3 and Muhammad Shabir Shaheen6
...tion individually and in combination on carcass yield, small intestinal microarchitecture and serum biochemistry in Hubbard chicks. A total of 128 one day-old chicks were divided equally in four different groups with each group having 4 replicates. The number of birds per group were 32 whereas the number of birds per replicate were 8.The first group was kept as control (CONT) whereas, the second (MOSG) and third (OABG) groups were given MOS (2g / kg of feed) a...
Muhammad Zamin* and Abdul Mateen Khattak
... for conservation and to combat salinity problem. For this purpose, various ecotypes of Sporobolus spicatus were investigated by conducting mowing tests in the year 2013 under UAE climatic conditions. Fifty ecotypes of Sporobolus spicatus were cut at various heights, i.e. 1, 2, 3, 4 and 5 cm. Significant differences were found among various ecotypes and mowing heights for different growth and quality parameters including green cover, canopy stiffness, leaf len...
Ted Peters
...ful way while studying a combination of natural history and human history? No, say big historians; we cannot ask the God question. Religion maybe, but certainly not God. It follows that we need more than just Big History which looks at nature through scientific lenses. We need Cosmic History to pose the God question, and even to discern human meaning within an otherwise impersonal universe. In this article, I entertain criticisms of my book, God in Cosmic H...
Imran Khan*, Zahir Shah, Wiqar Ahmad, Farmanullah Khan and Muhammad Sharif
... 10 and 15 t ha-1 FYM in combination with 75, 50 and 25% of the recommended NPK (INM5:75, 10:50, 15:25) in sub plot (5 * 3 m2) and tillage practices (TP) in main plots. Tillage practices effect on micronutrient status was significant, however, variations in physical properties were negligible. The Zn, Cu, Fe and Mn contents were 23, 11, 38 and 33% higher in ST than DT soil, respectively. Significantly higher OM in ST than DT soil is supporting these results wh...
Muhammad Ijaz Khan* and Amanullah Jan
...4 kg ha-1), alone and in combinations with gypsum (1000 kg ha-1) as inorganic soil conditioner were applied a week before sowing of maize. Design used was randomized complete block with split plot arrangement replicated thrice. Experimental site was Agronomy Research Farm, The University of Agriculture Peshawar. Irrigations regimes were subjected to main plots while soil conditioners to subplots. Results of the two years study indicated that most of the yield ...
Sumaira Zareen1, Syeda Sadaf Zahra2, Ayeza Mehmood3, Muhammad Asadullah4* and Aish Muhammad5
...erent concentrations and combinations of benzyl amino purine (BAP) or kinetin for shoot induction and Indole acetic acid (IAA) for primary shoot and Indole Butyric acid (IBA) for root proliferation. Best shoot proliferation (4.48 per explant) was observed in MS medium containing 0.2/0.4 mg/L BAP/IAA combination. For rooting of the micro shoots, MS medium was supplemented with 1.0 mg/l IBA exhibited best results with 4.69 mea...
Muhammad Aqeel Sarwar1*, Abid Ali2, Manzoor Hussain2, Saadia3, Muhammad Khubaib Abuzar4, Ijaz Ahmad5 and Sohail Latif6
...ed different fertilizers combinations i.e (F1) control, (F2)100% recommended dose of NPK, (F3) 75% recommended dose of NPK + 1.5 t ha-1 poultry manure(PM), (F4) 75% of recommended dose of NPK +3 t ha-1 farmyard manure (FYM) and(F5) 75% of recommended dose of NPK + 4.5 t ha-1 press-mud (PrM) along with seed inoculants (I1) Azospirillum and (I2
Sikander Khan Tanveer, Shaheena Yasmin, Imtiaz Hussain, M.Yaqub Mujahid*, Muhammad Munir and Muhammad Asif**
...the effects of different combinations of fertilizer nitrogen (N) and phosphorous (P) with or without farm yard manure (FYM) on yield, yield components and as well as on the grain quality parameters of wheat. Nutrient treatments included full fertilizer NP (100:100:00), full fertilizer NP + FYM (100:100:20), half- fertilizer NP (50:50:00), half- fertilizer NP + FYM (50:50:20), FYM only (00:00:20) and control (00:00:00). N & P were applied in kg ha-1 and FYM...
Rahmat Ullah Khan, Abdur Rashid*, Mohammad Sohail Khan** and Erdogan Ozturk***
...1 or 1.5 kg ha-1) and in combination with full (60:40 kg ha-1) and half (30:20 kg ha-1) the recommended rates of NP fertilizers. Results showed that in the first growing season (2007-08), the combination of 3 kg ha-1 HA with half (30:20 kg ha-1) rate of NP produced the highest grain yield (1314 kg ha-1) and increased the yield by 46.9% over the control. In the second growing season (2008-09), application of 3 kg ha-1 HA alon...
Abdullah Adil Ansari* and Kumar Sukhraj**
... the vermitech in varied combinations for exploring the effect on soil and productivity of Okra (Abelmoschus esculentus) in Guyana. The soil quality was monitored during the experiment along with plant growth parameters of Okra. The study revealed that combination organic fertilizers vermicompost and vermiwash combination [VW+VC] compared with control [CON] and chemical fertilizers [CHM], ...
Oguz Bilgin, Kayihan Z. Korkut, Alpay Balkan, and Ismet Baser*
...arents and their genetic combinations for the characters. The degree and direction of heterosis varied for different characters and for different hybrids. The highest heterosis over mid and better parents (120.14-109.93%) was recorded in hybrid of Kyzyltan91xIDSN209 for grain weight/spike. Maximum limits of heterosis over mid and better parent for spike length, spikelets/spike, grains/spike, spike density, grains/spikelets and spike harvest index were 8.77-8.1...
Saba Rafique1,2,*, Khalid Naeem1, Naila Siddique1, Muhammad Athar Abbas1, Aamer Ali Shah2, Akbar Ali1, Abdul Rahim1 and Farooq Rashid1
...ked to be derived from recombination. It showed maximum nucleotide homology 99.5% with ck/CH/LHB/121010 (KP036503), India/IBV572 (KF809797) and Japan/JP/Wakayama-2/2004 (AB363951.2) and 99.3% with 4/91 vaccine (KF377577), Iran/491/08 (HQ842715) and 99.1% with India/NMK/72/VRI/10 (HM748585) and was least related to rest of IBV lineages compared in this study. It is concluded that Pak-786 isolate belongs to GI-13 lineage that include both the vaccine and virulen...
Anish Shrestha
...diture on sugar drug and comb foundation seems to have positive and significant relation with gross return. All of them appeared to be underutilized and needed to be increased by 39%, 74% and 34% respectively for the profit maximization. Main production problem found to be high cost and inadequate availability of modern equipment with the index score of 0.81. And, according to the farmers, main role institution that should play is providing training to the far...
Muhammad Kashif Maan1, Muhammad Arif Khan1, Shehla Gul Bokhari1, Muhammad Ijaz2, Hamid Akbar1, Sajid Umar 3 and Muhammad Luqman Sohail4,*
...ts was anesthetized with combination of xylazine (3mg/kg) and ketamine (10mg/kg, I/V), while the second group (n=8) was pre-anesthetized with xylazine, followed by treatment with isoflurane (4%) with oxygen flow rate of 1L/min. Rabbits administered with xylazine-ketamine anesthesia showed significant increase (P<0.05) in serum alanine aminotrasferase, aspartate transaminase and blood urea nitrogen as compared to isoflurane anesthesia; whereas, no statistica...
Imtiaz Hussain*, Hassnain Shah, M. Azeem Khan, Waqar Akhtar**, Abdul Majid*** and M. Yaqub Mujahid*
Corresponding author:izhussain@yahoo.com
PRODUCTIVITY IN RICE-WHEAT CROP ROTATION OF PUNJAB: AN APPLICATION OF TYPICAL FARM METHODOLOGY
... wheat is harvested with combine followed by burning of residues that not only create environmental pollution but also affect the productivity of the cropping system. Stagnation in yield of rice and wheat is reported mainly due to uninterrupted sequential rice-wheat cropping which cause low fertility, increased weed problem, more disease and insect invasion leading to yield losses. Berseem is the only crop that breaks this rotation. Farmers are aware of the be...
Nazakat Nawaz, Malik Shah Nawaz*, Nasir M. Cheema** and Mubashir A. Khan*
Corresponding author:nazakat_nawaz@yahoo.com
ZINC AND IRON APPLICATION TO OPTIMIZE SEED YIELD OF MUSTARD
...n ha and 1.5 kg Fe ha in combination at the time of sowing. It can therefore be concluded that 100 % -1 seed yield of mustard variety BARD-1 increased at 5 Zn: 1.5 Fe kg ha as a -1 -1 result of increased pods plant , number of seeds pod and 1000-seed weight....
Amanullah, Ahmed Aziz Kurd, Saifullah Khan, Munir Ahmed and Jahangir Khan*
Corresponding author:aman_azrc@yahoo.com
BIOFERTILIZER- A POSSIBLE SUBSITUTE OF FERTILIZERS IN PRODUCTION OF WHEAT VARIETY ZARDANA IN BALOCHISTAN
...1 -1 of biofertilizer in combination with N 45kgha and P O 30 kgha increased 2 5 fresh yield from11% to 59% and suggested increases in grain yield of 20-46% as compared with control treatment....
Hidayatullah, T. Mahmood, M. Farooq, M. A. Khokhar and S.I. Hussain*
* Corresponding author: hidayatu2003@yahoo.uk.co.uk
PLANT GROWTH REGULATORS AFFECTING SEX EXPRESSION OF BOTTLE GOURD (LAGENARIA SICERARIA MOLINA) PLANTS
...iar spray with different combinations of ethrel did not improve yield and yield contributing attributes....
Muhammad Arifullah, Muhammad Munir*, Abid Mahmood**, Saifullah Khan Ajmal and Ghulam Shabbir*
COMBINING ABILITY ANALYSIS OF SOME YIELD ATTRIBUTES IN INDIAN MUSTARD (BRASSICA JUNCEA L.)
... crossed in all possible combinations using 8×8 diallel system for combining ability analysis. Data was recorded for seed yield and some important yield attributes. The results for combining ability analysis indicated significant general combining ability for most of the traits except plant height and siliqua length. UCD-8/4, KJ-119 and BRS-2 were ...
Zammurad Iqbal Ahmed, Muhammad Ansar*, Ashiq Saleem**, Zafar ullah Arif*, H.I. Javed and Rashid Saleem**
IMPROVEMENT OF MASH BEAN PRODUCTION UNDER RAINFED CONDITIONS BY RHIZOBIUM INOCULATION AND LOW RATES OF STARTER NITROGEN
...III and the treatment -1 combinations applied were control, 20, 40 and 50 kg N ha , 750 g inoculum -1 -1 -1 - ha , inoculum + 20 kg N ha , inoculum + 40 kg N ha , inoculum + 50 kg N ha 1 . The experiment was laid out in a split plot design keeping varieties in main plots and fertilizer/inoculum combinations in sub-plots. The results showed significant differences for all the plant characters under the study. -1 The crop matu...
Gulzar S. Sanghera and Wassem Hussain*
HETEROSIS IN RELATION TO COMBINING ABILITY PER SE PERFORMANCE IN TEMPERATE RICE (ORYZA SATIVA L.)
...;">Thirty six (36) cross combinations developed by crossing 2 Cytoplamic male sterility (CMS) lines with 18 testers in line x tester mating design were evaluated to find out best heterotic combinations in terms of yield and yield components of rice (Oryza sativa L.). Analysis of variance revealed significant differences among genotypes, crosses, lines, testers and line x tester interactions for all the traits studied. The sp...
Safdar Ali, Sahiba, M. Azim Malik, Fayyaz-ul-Hassan and M. Ansar*
GROWTH OF RAINFED FODDER MAIZE UNDER DIFFERENT LEVELS OF NITROGEN AND PHOSPHORUS
... yield, respectively. In combination -1 of nitrogen and phosphorus @ 180:90 NP kg ha , there was a significant increase in green fodder and dry matter yields through their interactive effect, that were 90.19% and 159.31%, respectively. This increase may be due to increase in vegetative growth parameters like plant height, stem diameter and leaf area. It was concluded that fertilizers combination of -1 180:90 NP kg ha was rec...
Wabekwa, J.W., I.A. Sodagni and F.K. Mohammad*
PHYSIOLOGICAL GROWTH AND YIELD EVALUATION IN PMINERALIZED SUNFLOWER (HELIANTHUS ANNUS L.) UNDER SUDANOSAHELIAN AGRO-ECOLOGY, NIGERIA
...AS in both years and the combined means for the two years, and at 8 WAS in 2011 and the combined analysis. Days to 50% flowering and head dry weight were not significantly influenced by P O rates and 1000- 2 5 -1 grain weights indicated that increased P O application (40-80 kg ha ) 2 5 enhanced grain filling efficiency and this influenced weight per 1000 -1 grains during both years. The application of 40 kg P O ha increased ...
Tahir Abbas, Bushra Nawab, Rahila Nazli, Rubina Saleem, Amrat Lal and Khalid Jamil*
EFFICACY OF COTTON WASTE COMPOST AND FERTINEMAKIL FERTILIZER ON THE GROWTH PARAMETER OF SUNFLOWER PLANTS
...ste compost alone and in combination with Fertinemakil fertilizer on sunflower crop was tested in the field of PCSIR Laboratories Complex Karachi, Pakistan. Individually all the treatment showed maximum plant growth character in terms of plant height, number of flowers, diameter of flowers, 1000 grains weight and oil contents. The highest significant improvement in plant growth characters were recorded in plots treated with cotton waste compost and cotton wast...
Nasia Batool*, Imtiaz Ahmad Qamar**, Imdad Hussain Mirza** and Muhammad Fateh Ullah Khan**
MIXING LESS PALATABLE GRASSES WITH UREA, MOLASSES AND EFFECTIVE MICROORGANISMS AND ITS EFFECT ON CHEMICAL COMPOSITION AND DIGESTIBILITY IN GOATS
...d (DB) were used and the combinations were grass + 4% molasses, grass + 4% urea, grass + 4% urea + 4% molasses, grass + 4% urea + 1:100 EM, grass + 1:100 EM + 4% molasses, grass +1:100 EM + 4% molasses + 4% urea. Proximate analysis of samples was carried out. Crude protein content of mixtures improved as compared with sole grasses. Digestibility of HC supplemented with urea, molasses and EM in various combinations was also s...
Sadia Tehrim*, M. Yasin Mirza** and G. Mustafa Sajid*
COMPARATIVE STUDY OF DIFFERENT GROWTH REGULATORS FOR EFFICIENT PLANT REGENERATION IN GRAPES
...tudy, different hormonal combinations in regeneration media were studied to determine the best combination for in vitro morphogenesis of grapevine accessions viz., 4494(1) Kowar Sufaid, 4472(12) Harker Agoon and 4492(1) Kowar Naray. When levels of benzylaminopurine (BAP) were increased in the basal MS media for culturing the grape plantlets, there was a corresponding increase in the number of shoots and root mass. Additional...
Syeda Nasreen*, Mehwish Ishaque**, Muhammad Ayub Khan*, Saleem-ud-din* and Syeda Musarrat Gilani*
COMBINING ABILITY ANALYSIS FOR SEED PROTEINS, OIL CONTENT AND FATTY ACIDS COMPOSITION IN SUNFLOWER (HELIANTHUS ANNUUS L.)
...53 were the best general combiners for seed total proteins, oil content, oleic and linoleic acid, respectively. Among males, C-206-R, SF-187R, RHA-295 and RHA-854 were the potential parents exhibiting desirable GCA for seed total proteins, oil content, oleic and linoleic acids, respectively. Among the F hybrids, 1 highest specific combining ability (SCA) estimates were exhibited by CMSNDMTC x RHA-295 (3.837) for seed total p...
Frasat Saeed*, Jehanzeb Farooq*, Abid Mahmood**, Tassawar Hussain***, Muhammad Riaz* and Saghir Ahmad****
GENETIC DIVERSITY IN UPLAND COTTON FOR COTTON LEAF CURL VIRUS DISEASE, EARLINESS AND FIBER QUALITY
...n cluster 1 and 5 may be combined to obtain desirable traits related to earliness and better disease tolerance. Scatter plot and tree diagrams demonstrated sufficient diversity among the cotton accessions for various traits and some extent of association between various clusters. It is concluded that diversity among the genotypes could be utilized for the development of CLCuD resistant lines with increased seed cotton and lint yields with desirable fiber quali...
Nosheen Zahra*, Ghulam Sarwar* and Sher Muhammad**
COMPARISON OF GYPSUM AND POTASSIUM SILICATE FOR RECLAMATION OF SALINE SODIC SOIL
...ssium silicate and their combinations at different levels for reclamation of saline sodic soil was tested. Treatments were replicated thrice. All pots were arranged according to completely randomized design and treatments were applied to the soil according to treatment plan. Appropriate time was given to achieve the reclamation of saline sodic soil and relative efficiency was assessed through laboratory analysis. After this, rice seedling (Shaheen Basmati) was...
Muhammad Arshad Ullah*, Nazir Hussain**, Helge Schmeisky*** and Muhammad Rasheed****
INOCULATION AND INTER-CROPPING OF LEGUMES IN ESTABLISHED GRASS FOR INCREASING BIOMASS OF FODDER
...3.70 and 17.87 t ha when combined with inoculation. Intercropping of grass and legumes 67% with inoculation was assessed as the best treatment. The increases were computed as 304%, 230%, 132%, and 60% over grass alone in the first, second, third and fourth crops while respective increases were 101%, 151%, 165% and 74% over monoculture legumes....
Tosif Tabassam*, Tariq Sultan**, M.Ehsan Akhtar**, M.Mahmood-ulHassan** and Arshad Ali**
SUITABILITY OF DIFFERENT FORMULATED CARRIERS FOR SUSTAINING MICROBIAL SHELF LIFE
...e rhizosphere. Different combinations of material were prepared using clay soil (35-50%), fly-ash (30-45%), press mud (5-15%) and lignite (5-15%). Clay soil (53% clay) was used for adhesion purpose but considering free of lump formation an important property of a good carrier, mixing 40% of soil with other material was found suitable. Using 40% of soil, six different treatments were formulated and physico-chemical characteristics were determined. Four
Syed Asif Imran Shah*, Shah Jehan Khan**, Abdul Aziz Baloch*** ,
Obaid Ullah Sayal**, Kalim Ullah* andShujaat Ali**
COMBINING ABILITY ANALYSIS IN VARIOUS INBRED LINES OF MAIZE (ZEA MAYS L.)
...p;An experiment to study combining ability effects through 6 × 6 diallel model in maize inbred lines, was laid out in randomized complete block design with three replications at Agricultural Research Institute, Dera Ismail Khan, Pakistan, during 2012. Variance analysis exhibited highly significant (P≤0.01) variation among genotypes for days -1 -1 -1 to 50% silking, ear length, kernel rows ear , kernels row , kernels ear and grain yield. Mean square va...
Muhammad Arshad Ullah*, Nazir Hussain**, Helge Schmeisky*** and Muhammad Rasheed****
IMPROVING FODDER QUALITY OF PANICUM GRASS THROUGH INTERCROPPING OF LEGUMES AND THEIR INOCULATION
...culation proved the best combination. The 6-7% higher crude protein (CP) of mixed fodder was recorded from 67% intercropping in comparison to grass alone while inoculation increased it by further 1-2%. Results also suggested that total digestible nutrients (TDN) were increased by 2-4% as compared to other treatments....
Amjad Pervez*, Syed Muzaffar Ahmed*, Akhlaq Ahmad** and Qazi Mehmood Ali**
EVALUATION OF DIFFERENT FOOD GRAINS AS BAITS FOR MANAGEMENT OF HYSTRIX INDICA
...cracked form alone or in combination with wheat can be applied as very palatable (32% and 27%, respectively) and cost effective grain as bait base for effective management of Indian porcupine, Hystrix indica, being an economically important rodent pest of agriculture, forestry and irrigation network in Pakistan....
Syeda Nasreen*, Muhammad Ayub Khan*, Muhammad Arshad*, Maryam Kamal**, Doulat Baig *, M. Tariq*, Talat Gilani* and Musarrat Gilani*
COMBINING ABILITY STUDIES FOR SEED YIELD TRAITS IN SUNFLOWER
...ents with high general 1 combining ability (GCA) effects were identified for yield related traits. Among
females, CMS-H55-2-2-1 expressed the highest significant GCA effects for 1000- seed weight whereas female, CMS-303 represented the highest and significant GCA estimates for head diameter, yield and harvest index. Among male parents, RHA-295 expressed the highest significant magnitude for head diameter while SF-187R possessed the highest sig...
Doulat Baig*, Fida Mohammad Abbasi*, Habib Ahmed*, Maqsood Qamar** and Muhammad Ayub Khan**
RESPONSE OF SUNFLOWER HYBRIDS TO DIFFERENT NITROGEN LEVELS FOR PHYSIOLOGICAL AND AGRONOMICAL TRAITS UNDER FIELD CONDITIONS
... ha can provide the best combination for good yield in sunflower crop under the prevailing humid conditions of Pakistan....
Waqar Akhtar*, Muhammad Sharif**, Abdul Hayee Qureshi*, Khalid Mahmood Aujla*** and Muhammad Azeem Khan*
COMPETITIVENESS OF TOMATO PRODUCTION IN PUNJAB, PAKISTAN
...ent (EPC) indicated that combine effects of policies on output and tradable input market did not pass any protection to tomato farmers in the study area. Net effect of policy or market failure is reducing the profitability of tomato producers at farm level which indicates lack of motivation from policies for farmers to expand tomato production as import substitute crop. Present study recommended competitiveness and economic efficiency analysis in other tomato ...
Hafiz Sultan Mahmood*, Tanveer Ahmad*, Zulfiqar Ali*, Munir Ahmad** and Nadeem Amjad***
FIELD EVALUATION OF A WHEAT STRAW CHOPPER
...gy that makes chaff from combine-harvested wheat straw and saves the environment from smoke pollution, if straw is burnt in the field. Field performance of this technology has not been evaluated yet, although this technology has been adopted in Pakistan for many years. The objective of this study was to evaluate the performance of an improved version of wheat straw chopper in combine-harvested wheat fields of southern Punjab...
Sadaf Javaria*, Muhammad Qasim Khan*, Kashif Waseem*, Muhammad Saleem Jilani*, Habib ur Rahman*, Muhammad Sohail Khan* and Muhammad Sohail**
EXPERIMENTAL INVESTIGATION ON IMPACT OF BIO AND ORGANOMINERAL FERTILIZES ON COMPOSITION AND SHELF LIFE OF TOMATO ( ) FRUIT.
...eriment was conducted by combining 7 treatments in completely randomized design with 3 replications. The results revealed that nutrient sources significantly affected different quality parameters including; physicochemical (vitamin C, lycopene, total soluble solids and pericarp thickness); sensory (fruit color, fruit flavor and fruit firmness) and shelf life. It was concluded that bio-fertilizer integrated with Farmyard Manure emerged as better nutrient source...
Nazia Qamar1, Sher Khan Panhwar1,* and Ralf Riedel2,*
...
Port landing of Scomberoides commersonnianus and S. tol were obtained between July 2013 and June 2015 for stomach content analysis. Analysis of prey composition was done using permutational analysis of variance (permanova), with species, life stage (juvenile and adults), gender, and weather (rainy and dry season) as factors. Patterns of empty stomachs were investigated to estimate feeding intensity. Feeding intensity was estimated with logis...
Basit Zeshan1,2,*, Mushtaq A. Saleem2,Javed Iqbal Wattoo2, Mohd Mokhtar Arshad1 and Maizan Mohamed1
...uct by sequencing. The recombinant plasmid was identified by restriction enzyme and sequencing analysis. The in vitro expression of the truncated protein was analyzed in E. coli with a molecular weight of 38kDa determined through SDS-PAGE and confirmed by Western blotting. The recombinant truncated protein was then purified from the culture media. The immunogenicity of the protein was studied in an animal...
Muhammad Umair Sattar1, Aysha Sameen1,*, Nuzhat Huma1 and Muhammad Shahid2
...s planned to investigate combined impact of fat mimetic (guar gum) and homogenization as fat reduction strategies on the quality of low fat Mozzarella cheeses (LFMCs). Guar gum was used at level of 0.15, 0.30 and 0.45% with and without cheese milk homogenization (2000 psi at 45°C) for LFMCs production. LFMCs produced were subjected to analysis for physicochemical parameters, functional attributes, texture (hardness), appearance (L* value) and proteolysis b...
Sabah Mansoor1, Muhammad Tayyab1,*, Amna Jawad1, Bushra Munir2, Sehrish Firyal1, Ali Raza Awan1, Naeem Rashid3 and Muhammad Wasim1
...nd characterization of recombinant α-amylase (AMYSBS) from Geobacillus SBS-4S. AMYSBS exhibited a highest identity of 99.78% with Geobacillus thermoleovorance GTA. E.coli BL21-CodonPlus (DE3) cells were used as host for expression studies of AMYSBS. Recombinant AMYSBS produced as inclusion bodies was transmitted to soluble active form by denaturing the in...
Kaori Sasaoka1, Takahiro Kataoka1, Norie Kanzaki1, Yusuke Kobashi1, Akihiro Sakoda2, Yuu Ishimori2 and Kiyonori Yamaoka1,*
...appropriate dose of CDDP combined with radon inhalation for highly radiosensitive mice. CDDP was administered at 20 mg/kg weight to C57BL/6J and BALB/c mice after radon inhalation at 1000 Bq/m3 and 2000 Bq/m3 for 24 h. Radon inhalation had a slight positive effect against CDDP toxicity in C57BL/6J mice with respect to improved hair condition, whereas radon inhalation exacerbated CDDP-induced toxicity in BALB/c mice, such as a decrease in ...
Ehtisham Shakeel Khokhar*1, Amir Shakeel1, Muhammad Amir Maqbool1, Muhammad Khubaib Abuzar2, Sumaira Zareen2, Syeda Sana Aamir4 and Muhammad Asadullah4
Zahida Parveen1, Safdar Sidra1*, Bushra Nisar Khan2
...ek, all three diets were combined and offered to the peafowls. The feed was pre-weighed and the leftover feed was collected and weighed the next day before offering the new feed. According to previous reports at site, the peafowls were preferred to poultry feed but in present research, the diet preference was in order of maize >millet >poultryfeed. During feeding, the behavioral patterns of the captive peafowls were monitored on daily basis. Time spent i...
Azhar Mehmood1,2,*, Muhammad Farrukh Saleem2, Muhammad Tahir2, Muhammad Aqeel Sarwar3, Tasawer Abbas4, Ali Zohaib2, Hafiz Tassawar Abbas5
...conducted to examine the combined effect of nitrogen and boron on growth, yield and oil quality of sunflower hybrids during 2009. Two nitrogen levels (0 and 150 kg ha-1) and three boron levels (0, 2 and 3 kg ha-1) were tested against two sunflower hybrids (Hysun-33 and DK-4040). All the growth and yield components like plant height, stem diameter, head diameter, achene and biological yield were significantly affected by varying levels of nitrogen and boron fer...
Muhammad Iftikharul Husnain1, Aneel Salman1, Inayatullah Jan2*, Tahir Mahmood3
...ld pay more attention to combat negative impacts of rising temperature. This will have an impact on the agriculture and hence on the livelihood of the mass population residing in South Asian countries. ...
Habib-ur-Rehman1, Masood Ahmed Siddiqui1,*, Abdul Qayyum1, Arifa Bano1 and Naeem Rashid2
Muhammad Shahbaz Aslam1, Iram Gull1, Zaigham Abbas2,* and Muhammad Amin Athar1
...n on target cells. The recombinant expression of some proteins in E. coli produces inclusion bodies which are misfolded proteins. The objective of this study was soluble expression of IFNα2-Tα1fusion protein in E. coli using pET-SUMO vector and determination of its biological activity. SUMO-IFNα2-Tα1 was successfully expressed in soluble form with IPTG induction to final concentration 0.5 mM at 37oC for 4 h and ...
Muhammad Adnan Khan1*, Majid Suhail Hashmi1, Ali Muhammad1, Muhammad Muneeb1, Haris Bilal1 and Gul Wali2
...uash was prepared by the combination of pulp: sugar: water, with 3: 4: 1 ratio respectively. All samples were tested for total soluble solids (TSS), pH, titratable acidity, reducing sugar, non-reducing sugar, ascorbic acid and sensory properties as flavor, color and overall acceptability. Samples were stored for three months and readings were recorded at every 15 days interval. During storage intervals, TSS increased from 48.0 to 51.31°Brix, pH increased 3...
Muhammad Ismaeel1,2*, Syed Mehar Ali Shah1, Aziz Ur Rahman1 and Mian Ahmad Raza1
... (45.1%) while F1 hybrid combinations Bas-2008/Kashmir-Bas and Bas-6129/Dokri-Bas for 1000-grain weight (18.9%) and grain yield (50.0%). Rice parents IR-8, Bas-6129 and Bas-370, based on their excellence for yield and yield related parameters, are recommended for use in future rice hybridization programs while the F1 cross combinations DR-92/DR-83, Bas-6129/Dokri-Bas and Bas-2008/Kashmir-Bas are recommended to develop advanc...
Sumaira Maqsood1,*, Muhammad Afzal2, Muhammad Anjum Aqueel2, Waqas Wakil3 and Hafiz Azhar Ali Khan1,*
... evaluated (alone and in combination) against S. litura under field condition. Minimum plant infestation (7.60±0.40%) was observed three days after application when B. thuringiensis and flubendiamide were applied in combination (@0.5 kg/ha+75ml/ha, respectively). Maximum infestation (11.60±0.97%) was observed in SpltNPV @ 1.0×109 POB/ml + B. thuringiensis @1.00 kg/ha. Simil...
Zihong Chen1, Ling Xu2, Xiaona Yang2, Yaguan Zhang3* and Yuming Yang4
...morphological characters combined with a multigene phylogenetics analysis involving 5.8S–ITS, nrSSU, nrLSU, EF–1α, RPB1 and RPB2. In multilocus phylogeny, M. baoshanense was grouped as a sister clade to Metarhizium majus, Metarhizium guizhouense, Metarhizium lepidiotae and Metarhizium indigoticum, but conclusively separated from these allied species with high support value. Morphological character differenti...
Changqing Zhao*, Zhuochi Chen and Yubin Li
...strated that the strains combined with Lactobacillus bulgaricus and Angel Yeast reduced the meat pH, degraded the protein in the meat by the proteases (including acid and neutral proteases) produced during fermentation, and increased the amount of free amino acids and flavored compounds. In these ways, the meat texture and taste were improved, and digestion and absorption were enhanced.
...
Abdul Basit1, Muhammad Zeeshan Majeed1,4*, Sohail Ahmed2, Gohar Ali2 and Muhammad Javaid3
...It is concluded that, in combination with biorational insecticides such as spinosad, installation of different colored refractive sheets, except yellow, at 30° or 60° angles would be more effective and economical to control fruit fly incidence on different vegetable crops such as M. charantia. ...
Fangxiao Dong1,2, Xuan Luo1,2, Delu Yuan1,2, Aixin Liang1,2* and Liguo Yang1,2*
...n DNA vaccine. A novel recombinant plasmid pEGS/2SS-tPA encoding two copies of somatostatin genes fused to tPA signal sequence was succesfully constructed and identified in MCF-7 cells. Subsequently, thirty female mice randomly were divided into three groups, treating with 50 μg pEGS/2SS-tPA (group T1), 50 μg pEGS/2SS (group T2), and 50 μg pEGFP-N1 (Control group) at weeks 0, 2, 4 and 6. Mating with male mice was performed at 2 weeks post final...
Muhammad Fiaz1*, Muhammad Afzal1 and Muhammad Zeeshan Majeed1,2
...ither alone or in binary combinations. There was a significant reduction in psyllid population for all treatment combinations as compared to control (F8, 107 = 70.36; P-value = 0.001 for 2016 and F8, 107 = 63.58; P-value < 0.001 for 2017). Combined application of spirotetramat along with I. fumosorosea formulation has shown a significant synergistic effect against D. citri infestation a...
Muhammad Shoaib Saleem*, Muhammad Faheem Akbar, Amjad Sultan and Saqib Ali
... Clothianidin, Momentum (combination of Nitenpyram+Chlorfenapyr) and Buprofezin were applied when population of insect pests reached at economic threshold level (ETL). Pre-treatment data were taken before 24 hours and post-treatment data were recorded after 24, 72, 168 and 336 hours of each spray. In this manner the data of three sprays were collected. Amongst neonicotinoids Clothianidin was found to be very effective with 84% reduction in jassid population ev...
Zartasha Anjum1*, Rakhshindah Bajwa2 and Munazza Hassan3
... when these were used as combination therapy along with tamoxifen in estrogen receptor (ER) and HMG-COAR positive breast carcinomas. However, in our local population, expression of HMG-COAR is not investigated. The objective of our study was to examine the expression of HMG-CoAR in primary breast carcinomas and determine its association with ER/PR expression.
Material & Methods: Cross-sectional analytical study was conducted to examine t...
Abdur Rehman and Zahir Shah
...a-1) applied alone or in combination on nodulation, nitrogen fixation, fresh pods yield and nutrient uptake of pea [Pisum sativum L.] under silt-loam alkaline soil conditions. Treatments were arranged in randomized complete block design (RCBD) with three replications. The required Fe and Mo as [(NH4)6Mo7O244H2O] and FeSO4.7H2O respectively were applied to soil followed by thorough mixing before sowing. Both Fe and Mo induced significant increases in fresh pods...
Rana Zeeshan Habib1, Muhammad Afzal1, Syed Zakir Hussain Shah2,*, Mahroze Fatima3, Muhammad Bilal4 and Syed Makhdoom Hussain5
...dividually as well as in combination.
...
Fiaz Ahmad1, Mumtaz Hussain2, Ghulam Abbas1, Muhammad Jawad Asghar1 and Muhammad Rizwan3*
...ore severely affected by combined lead and water stress treatment (80 mg Pb2+ 50% FC) as compared to single lead (40 mg Pb2+ 100% FC and 80 mg Pb2+ 100% FC) and single water stress treatment (0 mg Pb2+ 50% FC). The negative effect of Pb2+ and water stress on growth of shoot, root length, shoot, and root fresh and dry weight was observed more during 2nd harvest interval as compared to the 1st harvest interval. Highly significant differences were observed among ...
Shahen Shah1*, Manzoor Hussain1, Arshad Jalal1, Mohammad Sayyar Khan2, Tariq Shah1, Muhammad Ilyas1 and Muhammad Uzair3
...ted to main plots, while combination of sulfur (S) levels (15, 30, 45 kg ha-1) and application timings (100% at sowing, 100% at tillering and 50% at sowing + 50% at tillering) along with a control were allotted to the sub plots. Data regarding number of productive and unproductive tillers m-2, thousand grain weight, grains spike-1, biomass yield and value cost ratio (VCR) of wheat were significantly affected by N and S. Sulfur and its application time prominen...
Hafiz Manzoor Ahmad1,*, Hamad Bin Rashid1, Muhammad Arif Khan1, Asim Khalid Mahmood1, Muhammad Ijaz1, Shahid Hussain Farooqi1, Muhammad Rafi Ullah2 and Usman Ghani1
...with usage of anesthetic combinations. This study was aimed at comparative evaluation of two anesthetics combinations viz. ketamine-xyalzine (K+X) and ketamine-xylazine-diazepam (K+X+D). A total of 20 ducks presented at pet center University of Veterinary and Animal Sciences Lahore were divided into 2 equal groups each carrying 10 birds. The birds were monitored for various physiological parameters before and after an...
Muhammad Sajid Mahmood1*, Muhammad Ashraf1, Ijaz Ahmad2, Muhammad Khubaib Abuzar3, Sohail Latif4 Muhammad Aqeel Sarwar5 and Waqas Ahmad6
...ter extract alone and in combination with low doses of pendimethalin (pre-emergence) for weed control in mungbean during summer season, 2009. The treatments were: Control (weedy check), Hand weeding (30 and 45 DAS), Pendimethalin herbicide @ 1.5 kg a.i. ha-1 (recommended dose), Barley W.E. @ 12 L ha-1 (20, 30, 40 and 50 DAS), Barley W.E. + pendimethalin @ 12 L ha-1 + 1.0 kg a.i. ha-1, Barley W.E. + pendimethalin @ 12 L ha-1 + 0.75 kg a.i. ha-1, Barley W.E. + p...
Xiaqing Chen, Yü Huang and Zhen Huang*
...hiraiana alone or in combination with fungicide carbendazim under laboratory conditions. The results showed that S. shiraiana was not detected on mulberry leaves and branches, whereas it was only detected in mulberry fruits. T. hamatum fermentation broth and its ethyl acetate extracts caused high mycelial growth inhibition and germination inhibition of S. shiraiana, with the inhibition value up to 80% and 90%, respectively. An a...
Ghulam Abbas1, Khalid Pervez Akhtar1, Muhammad Ahsan2, Muhammad Jawad Asghar1, Fiaz Ahmad1 and Muhammad Rizwan3*
...es; mash interspecific recombinants. In this study, 72 mung × mash interspecific recombinant genotypes and a susceptible mungbean variety Mung Kabuli were screened against Yellow Mosaic Disease (YMD) using disease severity ratings by visual scoring of symptoms to calculate percent disease index (PDI). Tested recombinants responded differently to the disease. None of the tested genoty...
Dan Yü, Chuchu Li, Yü Huang and Zhen Huang*
...enoconazole alone or its combinations against S. sclerotiorum. The results showed that T. hamatum could infect and destroy the mycelia and sclerotia of S. sclerotiorum by mycoparasitism. The cell-free culture supernatant and ethylacetate extracts from T. hamatum inhibited mycelial growth of S. sclerotiorum, with EC50 value of 0.54 µl/cm2 and 2.99 µg/ml, respectively. The sclerotia production ...
Arifa Mehreen1, Iram Liaqat2,Muhammad Arshad3, Muzzamil Waheed4 and Najma Arshad1,*
...hood of strains carrying combinations of genes involved in toxin production and/or host immune evasion. Highest resistance was observed against beta-lactam group followed by cephalosporin, lincosamide, tetracycline, macrolides and aminoglycosides. No resistance was observed against vancomycin and linezolid. Among genes involved in host immune evasion, Staphylococcus protein A (spa) was identified most frequently (81%) and proportions of capsular ...
Binbin Shan, Yan Liu, Changping Yang, Shengnan Liu and Dianrong Sun*
...nicillatus from four combined tissues (eyestalk, muscle, intestinal and viscus) using High-seq sequencing technology. A total of 65,703,216 high-quality clean reads were generated to produce 27,850 non-redundant transcripts with a mean length of 918 nt using Trinity and Tgicl software. For homological alignment, 13,083 unigenes had significant hits in Nr database. And then, 10,467 unigenes were annotated into three ontologies: biological processes, cellula...
Ali Sher1*, Khalid Naveed1, Gulzar Ahmad2, Ayub Khan1, Muhammad Saeed1 and Shah Masaud1
...er no fertilization thus combination of the 150 kg ha-1, 3 kg Zn ha-1 and 1 kg Fe ha-1 is recommended for improving biomass and leaf area. Further studies are recommended to understand the mechanistic approach of such improvement. ...
Gokhan Sahin1, Ecevit Eyduran2,*, Mete Turkoglu3 and Fatma Sahin2
Junaid Akhtar1,2, Sidrah Saleem1, Naveed Shahzad3, Abdul Waheed1, Iqra Jameel1, Farhan Rasheed4 and Shah Jahan5,*
...ied Hodge test (MHT) and combined disks diffusion (CDD) method, respectively. The MBL producing clinical isolates were further subjected to PCR for the existence of blaIMP and blaVIM genes. The carbapenem resistant A. baumannii (n=32), P.aeruginosa (n=26), K. pneumoniae (n=19), E. coli (n=16), C. ferundi (n=04), P. vulgaris (n=02) and E. cloacae (n=01), were isolated from clin...
Ashraf Khan1*, Suliman Shah2, Maid Zaman3 and Komal Habib2
...d in plants treated with combination of Nitenpyram + Chlorfenapyr (99.20 and 99.89 %) followed by Flubendiamide (89.83 and 99.39 %) and Imidacloprid (89.31 and 98.77 %) after spray 1 and 2, respectively. Least effective insecticide was proved to be Melathion with 39.79 and 65.43 percent reduction in citrus psyllidspopulation except untreated plants with 9.27 and 15.94 percent decrease after first and second spray, respectively. Decline in overall population re...
Madiha Naz1, Qudrat Ullah Khan1*, M. Jamil Khan1, Obaid Ullah Sayal2, Asim Afridi3 and Aziz Ullah Sayal3
...g manures and pressmud incombination with foliar spray of macro-nutrients and naphthalien acetic acid (NAA) and compared with inorganic soil applied fertilizers. The results of the study showed significantly higher number of branches, chlorophyll content in the treatment recieving inorganic fertilizers, the manures in combination with foliar NPK was comparable. The yield attributes showed that ten fruit weight were greater i...
Saif Ullah and Mohammad Akmal*
...d 30 kg ha-1 is the best combination for soils remained with cereal crops in cultivation to plant with sunflower good quality of oil and protein. ...
Safina Naz1, Khushbo Hussain1, Muhammad Asif Zulfiqar2* and Syed Atif Hasan Naqvi3
...id, Salicylic acid (SA), combination of Naphthalene Acetic Acid & salicylic acid and orange oil extract on physical and chemical parameters gave significant response. NAA promotes vine length upto 12.66 feet while chlorophyll content was 72.50 SPAD Value, while fresh weight of leaves is 5.1g, dry weight is 0.41g, while fresh weight of fruit is 121.66 g and fruit length is 14.11 cm. Salicylic acid promoted vine length upto 11.33 feet while chlorophyll conte...
Rahim Dad Brohi1,*, Amir Bukhsh Kalhoro1, Allah Bux Kachiwal1, Illahi Bux Kalhoro1, Dildar Hussain Kalhoro1, Sarfaraz Ahmed2, Farhan Anwar Khan3, Hira Sajjad Talpur1, Zia-ur-Rehman1 and Dinesh Bhattaria4
Shamsher Ali1, Zakir Ullah Jan1*, Zahir Shah2, Muhammad Jamal Khan2, Farmanullah Khan2, Inayat ur Rahman3 and Shah Fahad3
...eatments application but combined used of farm yard manure, N and P2O5 in (T5) and (T6) significantly increased yield and yield traits of Hybrid CS-200 and Azam variety than rest of the fertilizers treatments. The significantly maximum plant height of Azam cv. (260 cm) and hybrid (247cm) were recorded in treatment T5 (N- P2O5@ 130-90 kg per hectare + 20 ton per hectare FYM) followed by T6 (50-45) kg N, P2O5 ha-1 + FYM @ 20000 kg ha-1. The Azam plants were tall...
Yasir Ali1*, Muhammad Aslam Khan1, Muhammad Atiq1 and Muhammad Hussain2
Raza Ullah Khan*, Ahmad Khan, Mohammad Zameer Khan, Fayyaz Hussain and Sonia Saba
...r foliar or proportional combination thereof. Treatments consists of whole P applied as soil (PS =100%), foliar (Pf=100%) or different combination (PS75:Pf25, PS50:PF50, PS25:Pf75) to wheat grown in 5 kg soil in plastic container. Data on plant growth parameters (plant height, biomass and grain yield) was collected at the end of the experiment taking as an average of the three replications. Nutrients such as K, P and selecte...
Peng Xu1, Bin Liu1,2, Yongqiang Zhao3, Shicheng Lv3 and Changhu Lu1,*
Ammara Afzal1, Tazmeen Fatima1, Maham Tabassum1, Muhammad Nadeem2*, Muhammad Irfan3, Quratulain Syed2
...ong both strategies, SSF combination of T. viride and S. cerevisiae gave maximum ethanol production (3.88%) after 12 days of fermentation at 30ËšC.
...
Arshad Iqbal1*, Syed Mehar Ali Shah1, Hidayat ur Rahman1, Faiza Aman2 and Aziz-ur-Rahman1
...rived from F5 rice cross combinations along with their 8 parents were planted in a randomized complete block design (RCB) having three replications at The University of Agriculture, Peshawar during 2016 rice crop growing season. Data were taken on some of the morphological traits like number of days to heading (days), number of days to maturity (days), flag leaf area (cm2), culm length (cm), panicle length (cm), primary branches panicle-1 (no.) and secondary p...
Monsif Ur Rehman1*, Hidayat-ur-Rahman1, Muhammad Iqbal2, Iftikhar Hussain Khalil1 and Zahir Shah3
...was aimed at determining combining ability and grain yield performance of S1 lines derived from ten maize populations. These populations were crossed in half diallel design and the resulting 45 cross combinations were further explored for their use as source of elite inbred line development in a line × tester experiment conducted at Cereal Crops Research Institute, Pirsabak and Maize and Millet Research Institute, Yous...
Ambrin Rajput*
...g ha-1) were recorded by combined application of N, P and K fertilizer application. However, the minimum values were obtained from control treatment. The combined application significantly increased chickpea yield compared the one without K application. There was positive significant relationship between all yield parameters except straw yield. Hence, it was recommended that combined appli...
Syed Atif Hasan Naqvi1*, Ummad-ud-Din Umar1, Ammarah Hasnain2, Ateeq ur Rehman1 and Rashida Perveen1
...ither individually or in combination were tested at various concentrations under in vitro conditions by poison food and disk diffusion techniques while best effective seven were trialed in glass house and field experiments to determine their efficacy against Xanthomonas oryzae pv. oryzae. In vitro and in vivo studies under different conditions showed significant response of Mentha piperita, Azadirachta indica and Aloe vera either tested individually or in
*Muhammad Waseem1, Dost Mohammad Baloch1, Ghulam Khaliq1,
M. Rashid1, Qurban Ali2 and Mustajab A. Khan3
INTENSIFICATION IN BAMBOO (Bambusa bambos L.) TRAITS IN RESPONSE TO ORGANIC AND CHEMICAL NITROGEN ALONG THE COAST OF ARABIAN SEA
...application of treatment combination {50% N from urea (the recommended dose) + 50% N from Farm Yard Manure (FYM)}. It was suggested that the growing of bamboo should be produced through the use of combination of chemical {50% N from urea and organic N (50% N from Farm Yard Manure)....
Sumera Siddique1,†, Hafiz Abdullah Shakir1, Javed Iqbal Qazi1*, Amtul Bari Tabinda2, Muhammad Irfan1
Screening of some agri-wastes for economical cultivation of Candida tropicalis SS1
...l as in eleven different combinations. The media were inoculated with 1% (w/v) suspension of 24 h old yeast cultured in nutrient broth. The strain grew best in water melon (WM) at pH 7.0 and 37 °C under non agitation conditions. The proximate analysis revealed that the water melon peels showed the highest amount of crude protein (which was measured by micro Kjeldahl digestion system) followed by bagasse, mango, and apple peels with respective values of as ...
Hafiz Muhammad Umer Qaisar1, Tanveer Ahmad2, Muhammad Rizwan3*, Muhammad Saqib1
Antimicrobial efficacy of combination of lincomycin and spiramycin (Lispiracinâ„¢) as systemic dry cow therapy for controlling bovine mastitis
...etermine the efficacy of combination of lincomycin and spiramycin
(Lispiracin™) for controlling bovine mastitis through systemic dry period therapy,
present study was designed. A total of 20 dry pregnant cows were selected
randomly at the end of lactation and divided into two equal G1 and G2 groups.
Group G1 was treated with lincomycin@ 5mg/kg (IM) and spiramycin@ 10 mg/kg
(IM) (Inj. lispiracin®) at end of lactation...
(Lispiracin™) for controlling bovine mastitis through systemic dry period therapy,
present study was designed. A total of 20 dry pregnant cows were selected
randomly at the end of lactation and divided into two equal G1 and G2 groups.
Group G1 was treated with lincomycin@ 5mg/kg (IM) and spiramycin@ 10 mg/kg
(IM) (Inj. lispiracin®) at end of lactation...
Raazia Kiran1, Alya Riaz1, Muhammad Irfan1*, Hafiz Abdullah Shakir2
An overview of pre-treatment methods used for bioconversion of lignocellulosic biomasses into valuable products
...acteria, and quite a few combinations thereof to
breakdown the lignocellulose into its components. Pre-treatment process changes
cellulose morphological, chemical and physical features, making it prone to
enzymatic attack for saccharification. In this review, we have discussed different
pre-treatment methods and their effect on recalcitrant structure of lignocelluloses
for making it readily available for enzymatic hydrolysis&...
breakdown the lignocellulose into its components. Pre-treatment process changes
cellulose morphological, chemical and physical features, making it prone to
enzymatic attack for saccharification. In this review, we have discussed different
pre-treatment methods and their effect on recalcitrant structure of lignocelluloses
for making it readily available for enzymatic hydrolysis&...
Muhammad Asghar1*, Mirza Muhammad Qadeer Baig1, Sanaullalh Chaudhary1 and Muhammad Anjum Ali2
... crop by using different combinations of fungicide (difenoconazole) with various concentrations of sprayable formulations of macronutrients (NPK) was conducted during Kharif 2012 to 14 at Adaptive Research Farm, Gujranwala, Pakistan. The experiment was conducted on a promising basmati rice cultivars Super basmati in RCBD with three repeats. The spray of difenoconazole at rate of 315 mlha-1 + 500gha-1 of NPK (20:20:20) significantly decreased incidence of BLS p...
Misbahullah, Shad Khan Khalil, Asim Muhammad*, Afza Tabassum and Shah Fahad
...tract concentration with combination of weedicide sprayed at tillering stage gave less number of weeds (76), minimum weed fresh weight (170) and dry weight (132), increased days to heading (116.5), increased days to anthesis (123.0), increased days to physiological maturity (154.4), maximum number of tillers m-2(192.6) and more number of of spikelets spike-1 were found when treatments applied 50% at emergence + 50% at tillering or tillering stage. Days to head...
Shagufta Nighat1, Muhammad Sajid Nadeem1,*, Syed Israr Shah1, Amber Khalid1, Tariq Mahmood2, Ayesha Aihetasham3 and Muhammad Asif4
...d other activities like, combustion of fuel, processing of metals, coal mining, automobile, lead-acid batteries, and building material.Toxic metals are bio-accumulated by insectivorous bats due to their foraging habits and high trophic level. There is a growing interest on the conservation of bats throughout the world as they play an important role in controlling insect population. We measured cadmium (Cd), copper (Cu) lead (Pb) and zinc (Zn) concentrations in...
Qingling Hu1,2,3,* and Jinian Feng3
...que in this genus by the combinations of having 5 pairs of long setae on pronotum, the ultrashort chapped craspedum on posterior margin of abdominal tergites, and the shape and relative locations of setae on tergite IX.
...
Yan-Guo Han1, Xiao-Li Peng1, Kai Li1, Yu-He-Tian Zhao1, Xun-Ping Jiang2, Guang-Xin E1, Yong-Ju Zhao1, Jun-Hua Ye3, Li Xu3, Qin-Tao Zhao3 and Yong-Fu Huang1,*
...the CpG-SS fusion type recombinant plasmid named ptCS/2SS-asd. Twelve ram lambs were randomly divided into three groups, namely, the treatment (Group T1, ptCS/2SS-asd; Group T2, pCpG + ptS/2SS-asd) groups and the control (Group C, pVAX-asd). The vaccine (1 mg/ram) was injected into the rams at weeks 0, 3 and 6 of the study. The rams in Group T1 and T2 showed significantly higher anti-SS antibody, serum growth hormone and IGF-1 concentrations and average body w...
Syed Said Badshah Bukhari1*, Ghulam Ali Bajwa2 and S. Shafiqur Rehman1
...t the use of ring-widths combined with intra-ring wood formations enables better assessment of impacts of climate change on tree growth. It is recommended that climate change projections and scenarios may be made an integral part of Forest Management Plans for making realistic wood volume and yield estimates ...
Muhammad Saqib Shahzad1, Amina Arif1,*,Saeeda Kalsoom2, Javed Iqbal3, Shahnam Shafique2, Muhammad Shahid Nadeem4 and Rafique Ahmed5
...apeutic effectiveness of combined ribavirin (RBV) and interferon (IFN) therapy in hepatitis C virus (HCV) patients. Overall, 590 HCV patients were evaluated pre and post combined drug therapy during the period 2015-2017. Distribution of HCV genotypes, age groups, viral load and alanine aminotransferase (ALT) values were recorded and analyzed for rapid virologic response (RVR), non-rapid virologic response (NRVR), sustained v...
Mudassar Javed, Muhammad Zeeshan Majeed*, Muhammad Luqman and Muhammad Afzal
...digenous okra growers to combat infestations of E. vittella and H. armigera and other lepidopterous borers. ...
Zakiullah1, Muhammad Farid Khan1*, Muhammad Mohibullah3, Muhammad Iqbal2, Irfanullah3, Faheemullah3, Madiha Urooj1 and Uzma Arif1
...iment to determine their combining ability through 6 x 6 diallel mating design. The research was laid out in randomized complete block design with three replications at Cereal Crop Research Institute (CCRI) Pirsabak, Nowshehra (K.P.K) Pakistan during the year 2015. The results displayed highly significant (P≤0.01) variation among above six OPVS for days to germination, plant height, leaf area, number of grains ear-1, grains moisture % at harvest, grain yiel...
Raza Ullah1* and Jamal Shah2
... farmers using different combinations of the two risk coping tools namely, off-farm diversification and precautionary savings. The results suggested that adoption of risk coping tools, particularly the simultaneous adoption of multiple tools at the same time, significantly reduced wheat yield. The research suggested that future studies should identify ways to minimize such costs associated with risk management at farm level. ...
Muhammad Asad Saleem1,*, Mirza Abdul Qayyum1, Mudssar Ali1, Muhammad Amin2, Muhammad Tayyab1,3 and Sumaira Maqsood4
...ence was recorded in the combined treatments. Moreover, decrease in weight gain, frass production and cumulative gain in size was found when larvae were treated with integrated effect of Bb and Nit. Depending on the lethal of treatment, development duration of RPW was disturbed. Integration of Bb and Nit delays the development and diet uptake in RPW which can be used for agent of control for this cryptic insect.
...
Rahat Afza, Muhammad Afzal, Muhammad Zeeshan Majeed and Muhammad Asam Riaz*
...of different life stages combinations of Coccinella septempunctata and Coccinella transversalis in the presence and absence of prey and the impact of sublethal concentrations of insecticides on intra-guild predation (IGP) and aphid consumption by coccinellids. Data revealed that in the absence of prey larvae of C. septempunctata exhibited more predation on C. transversalis while the predation of C. septempunctata on C. tra...
Muhammad Waqas Imam Malik1, Khalid Usman1*, Tahir Amin1, Muhammad Riaz2, Zafar Iqbal3 and Fiaz Hussain1
...that variety CRIS-134 in combination with 25 days irrigation interval conserved 60 % more water, produced 60.5% more bolls per plant, 21.1% higher GOT, 48.7% higher seed cotton yield, and 12.4% higher fiber length compared to all other combinations. Results indicate that CRIS-134 with 25 days irrigation interval in zero tillage system is more advantageous and adaptable in agro-ecological conditions of Dera Ismail Khan <...
Sumaira Maqsood1,*, Muhammad Afzal2, Muhammad Saleem Haider1, Hafiz Azhar Ali Khan1, Muhammad Ali1, Muhammad Ashfaq1, Muhammad A. Aqueel2 and Muhammad Irfan Ullah2
...d alone and in different combinations against 2nd and 4th instar larvae of S. litura. The highest mortality of larvae was recorded after 7 days of application when both insect pathogens were used in combination (SpltNPV 1×108 POB/ml + Bt 0.1 μg/g) i.e. 85.25, 79.31 and 92.11% mortality (in case of 2nd instar larvae) and 70.42, 63.64 and 80.88% (in case...
Aisha Siddiqua1*, Munir Ahmad1 and Nusrat Habib2
...egies in isolation or in combination on yield. The primary data of ‘Climate Change Impact Survey 2012-13’ used for this purpose. Crop-specific strategies of change in the time of sowing, use of inorganic fertilizer, change in irrigation, and varietal change. Change in irrigation practice gave rise to the highest yield suggesting that this might be the necessary requirement to sustain wheat growth in Pakistan in future. Farmers are expected to recei...
Syed Amjad Kamal Jan* and Naushad Khan
...ral farm plots should be combined and modern farming be practiced on the farm for enhancement of rice productivity in the study area. ...
Huseyin Cetin1,*, Oner Kocak2, Emre Oz1, Samed Koc1, Yesim Polat1 and Kalender Arıkan2
...s used as a synergist in combination with SPs for the control of houseflies. The aim of this research was to determine the effect of SPs (cypermethrin, permethrin and deltamethrin) and their combination with different rates of PBO (1: 0.125, 1:0.25, 1:0.5 and 1:1) against four Turkish house fly populations (Ankara, Antalya, Gaziantep and Şanlıurfa) in laboratory conditions. According to results the use of PBO in
Naseer Ahmed Abbasi1, Mian Noor Hussain Asghar Ali1*, Bilawal Abbasi2, Shakeel Ahmed Soomro1, Najeeb Ahmed Khan Nangraj1, Jam Ghulam Mustafa Sahto1 and Sajjad Ahmed Morio1
...ies of different methods combined with fuzzy analytic hierarchy process (AHP) and the potential time and cost saving methods can assess the suitability of land. Finding shows that the fuzzy set method is different from parametric method. The influence of explicit weight on land characteristics is combined with the evaluation of land characteristics. The correlation coefficient is high for both indices, but results obtained w...
Hafiz Abdullah Akbar, Zafar Iqbal, Waqas Raza*, MuhammadUsman Ghazanfar and Salman Ahmad
...nt extracts alone and in combination with wax and 3 concentrations of salicylic acid alone and in combination with wax. The study revealed that in case of plant extracts maximum growth inhibition (35.90 %) was shown by Anar followed by Chabir (33.60%) and Garlic (29.16%), while minimum growth inhibition was recorded from Parthanium (17.43%) and Ginger (19.94 %). In case of plant extracts + wax maximum growth inhibition (44.3...
Hande Küçükönder1, Pınar Çelebi Demirarslan1,*, Aykut Burgut2 and Mustafa BoÄŸa3
...Making (MCDM) techniques combined with the data fusion technique support the proposed hybrid approach’s results. In this context, the proposed hybrid approach was applied to evaluate the monthly production performance of a commercial enterprise with Lohman Brown genus 8000 chickens. According to the findings of the analysis, it was seen that January, March, October, November and December are the months when production performance is high. When these mont...
Aisha Khalid1, Muhammad Tayyab1,*, Abdual Rauf Shakoori2, Abu Saeed Hashmi1, Tahir Yaqub3, Ali Raza Awan1, Muhammad Wasim1, Sehrish Firyal1, Zaheer Hussain4 and Munir Ahmad5
...nd characterization of recombinant thermostable cellulase from Thermotoga naphthophila. PCR using the genomic DNA of T. naphthophila as template resulted in amplification of 1 kb cellulase gene. The amplified cellulase gene was cloned in pTZ57R/T and sub-cloned in pET28a. The expression of recombinant cellulase was analyzed using BL21 CodonPlus (DE3) cells as expression host. The expression studies resulted in ...
Liyan Zhang1,2, Zhidong Zhou1,3, Haiping Li1,2, Yanlong Qiao4, 5 and Yueping Zhang1,3,*
...orical demography of Scomber japonicus collected from four geographical locations in the Taiwan Strait and its adjacent waters. The results of genetic diversity and genetic structure analysis of S. japonicus showed that these four populations exhibited moderate genetic diversity across the sampled geographical range of the species. However, no phylogenetic structure corresponding to geographic location was observed from the neighbor-joining (NJ) ...
Azam Ali* and Amjad Farooq
...otton should be grown to combat pest infestation....
Åžaban Çelebi
...enomethionine) and their combination on Se concentration in liver, heart, kidney and breast muscle. In addition, malondialdehyde (MDA) concentration and enzyme activities of glutathioneperoxidase (GSH-Px), superoxide dismutase (SOD) and catalase (CAT) in liver, heart, breast and thigh tissues of laying hens were determined in present study. A total of 96 White Lohman laying hens, aged 24 weeks, were randomly divided into 4 groups (n=24), each of which was comp...
Muhammad Shahid Nadeem*, Maryam A. Al-Ghamdi and Jalaluddin Azam Khan
... was PCR amplified and recombinant plasmid pET22b-Asn was prepared. L-asparaginase was produced in BL21 (DE3) RIL codon plus strain of E. coli as heterologous protein under 0.3mM IPTG. The recombinant enzyme was purified by DEAE-Sephadex colum based anion-exchange chromatography. On SDS-PAGE, the enzyme exhibited a molecular weight of about 36 KDa. Its specific activity was 1650 U per mg of protein with about 5% gluta...
Aisha Khalid1, Muhammad Tayyab1,*, Abu Saeed Hashmi1, Tahir Yaqub2, Ali Raza Awan1, Muhammad Wasim1, Shagufta Saeed1, Sehrish Firyal1 and Abdul Rauf Shakoori3
... maximal production of recombinant thermostable cellulase from Thermotoga naphthophila using BL21-CodonPlus (DE3) cells as expression host and pET28a as expression vector. Effect of various concentration of Isopropyl β-D-1-thiogalactopyranoside (IPTG), post induction time, effect of temperature and pH were examined for the maximal production of recombinant cellulase. The effect of supplementation of LB medium wit...
Fakhra Soomro1,*, Riffat Sultana2 and Muhammad Saeed Wagan2
...e differentiated through combination of characters i.e. coloration, morphometry, pronotum, femur bands, wing pad position and appearance of bands.
...
Syed Atif Hasan Naqvi*
...izing to help farmers to combat the disease. There is a need to use environmentally safe approaches to overcome the loss of grain yield in rice due to this disease. Climate change has a serious effect on this disease because in current scenarios cropping pattern has been changed and the stakeholders has shifted to some other crops rather the practicing ones. Since, farming community has adopted rice crop in non-core areas of Indian Sub-continent where it was o...
Aziz Ur Rahman* and Syed Mehar Ali Shah
...comprised 87 rice F5:7 recombinant inbred lines (RILs) along with three check cultivars namely Pakhal, Kashmir Basmati and Fakhr-e-Malakand. The genetic material was planted in alpha lattice design using three replications across different locations namely Peshawar, Swat, Manshera and Charsadda of the Khyber Pakhtunkhwa province of Pakistan during 2017. Pooled analysis of variance indicated significant (p≤0.01) differences among the environments, genotypes ...
Bushra Siddique1,*, Muhammad Tariq1, Muhammad Naeem1 and Muhammad Ali2
...reatments with different combinations of plant extracts and semiochemicals were used for natural enemypreference experiment. The experiment was conducted with seven treatments and five replications at Glass house situated in Pir Mehr Ali Shah Arid Agriculture University, Rawalpindi field area during Feb-April, 2015. The Coccinella septempunctata were collected from wheat crop plants. They remained starved for two days before Olfactometer bioassays. For ...
Fraza Ijaz1*, Umair Riaz2, Shazia Iqbal3, Qamar uz Zaman4, Muhammad Furqan Ijaz5, Hina Javed1, Muhammad Amjad Qureshi1, Zuhra Mazhar3, Ahmad Hassan Khan6, Hassan Mehmood7 and Ijaz Ahmad8
...sults indicated that the combined application of Rhizobium species and Tryptamine performed better by improving growth and yield and quality parameters. It is concluded that precursor-inoculum combination is an effective approach and should be tested in different ecologies. ...
Sheng Niu1, Ali-Raza Jahejo1, Fa-jie Jia1, Xin Li1, Guan-bao Ning1, Ding Zhang1, Hai-li Ma1, Wei-fang Hao2, Wen-wei Gao1, Yu-jun Zhao1, Shi-min Gao1, Jian-hui Li1, Gui-lan Li1, Fang Yan1, Rong-kun Gao1, Huan-chun Chen1,3 and Wen-xia Tian1,*
...ith different doses of recombinant glutathione-S-transferase A3 (rGSTA3) proteins. We explored the responses of HDPs in erythrocytes to thiram-induced TD and rGSTA3 protein by quantitative real time PCR (qRT-PCR). The results showed many HDPs expressions were suppressed by thiram-induced TD, and the expressions of HDPs were upregulated by rGSTA3 protein. These findings demonstrated that mRNA expressions of HDPs are highly related to thiram-induced TD, and rGST...
Pin Lyu1, Xiangxian Chen2* andQinlong Liu3*
Amjad Ali1*, Wiqar Ahmad1, Muhammad Zeeshan1, Farmanullah Khan2 and Muhammad Motasim Billah1
...; 2, 2-5 and 5-10 mm) in combination with two commercial biofertilizers; Bizote-N and Rhizogold to lentils during Winter 2015 in an RCB design and the residual effect was evaluated on soil micro-nutrient status and chemical properties after maize harvest in Summer 2016. Particle sizes in the above order showed 3, 2 and 2% increase in soil pH over the control (7.63) and 15, 10 and 6% increase in soil EC over the control (125 mS cm-1), respectively. Their OM co...
Zafar Abbas1*, Muhammad Mubashir1, Umair Riaz1, Zeenat Javid1, Muhammad Ashraf1, Saeed ur Rehman1, Muhammad Javid Qamar1, Syed Ali Zulqadar1 and Shahzada Munawar Mehdi2
Ammara Saeed* and Noor-ul-Amin
...kg ha-1) each as well as combined. Results showed that when plants were supplied with phosphorus at 60 kg ha-1 and potassium at 40 kg ha-1, it significantly improved studied variables, such as early days to sprouting (11.5 days), days to flowering (35.0 days), maximum number of branches plant-1 (11.4), number of leaves branch-1 (19.0), flower stalk length (44.7 cm), flower diameter (7.7 cm), number of flower plant-1 (12.2), flower persistence (11.6 days) and s...
Shafqat Husnain Khan1, Shah Jahan2, Irshad Ahmad3,4, Sadeeq ur Rahman5,* and Tayyab ur Rehman4,*
...enemase producers, while combined disc diffusion test identified 28 isolates as MBL producers among them. Of all carbapenemase producers, 25 isolates (30.8%) of carbapenemase-producing E. coli were found to harbor bla-VIM gene, while 13 isolates (16.04%) were found to carry blaIMP gene. We report on high incidence rate of carbapenemase producers among carbapenem resistant E. coli isolates.
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