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Timothy Helton

In a Mirror Dimly: Anthropology and Restoring a Sense of Presence to an Empty World
...ological conceptions and methods made by contemporary thinkers. Next, taking the work of E. B. Tylor as an example, I demonstrate the validity of such criticisms. Then, I offer two examples of alternatives to objectifying ethnography before tracing developments in anthropological method in the last decades that have greatly improved the lens used for the anthropological gaze. Finally, I conclude by suggesting the collaboration between anthropology and theology...

Chun-Yi Lin1, Meng-Ling Wu2, Tang-Long Shen1, Hsin-Hung Yeh3, Ting-Hsuan Hung1*

 

...plex molecular detection methods provide the understanding of the genetic diversities among viroid isolates and quantify viroids in citrus host. Our field survey can help clarify citrus-viroid relationships and develop proper prevention strategies in the near future.

 

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Rick Repetti

...fferent frameworks, methods, and modes of discourse that are distinctively Buddhist, much of which it presents in its own modality.

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Shumaila Manzoor1, Afshan Ahmed1 and Muhammad Abubakar2*

...y and specificity. These methods were performed by using set of sera collected from cattle with foot-and-mouth disease vaccination. The pattern of antibody titers in animals of different age groups was observed as less than 1 year, 1-2 years and more than 2 years for both ELISA and SNT. The peak immune response measured by SNT was 2.8log10 whereas ELISA detected serum antibody titer up to 2.4 log10. Findings showed tha...

Ummara Waheed Khan, Raza Ahmed, Irum Shahzadi and Mohmmad Maroof Shah

Email | mmshah@ciit.net.pk

...align: justify;">Various methods and techniques have been developed and tested to improve important cereal crop species since quite long time. Among the prominent techniques, traditional plant breeding has been on leading edge. The discovery of molecular genetics and biotechnological techniques, such as genome mapping, tissue culture, genetic transformation and in vitro regeneration offered new dimensions of research to improve important cr...

Mushtaq Ahmad1*, Habib Akbar1, Mohammad Tariq Jan1, Muhammad Jamal Khan Khattak2 and Abdul Bari3

...ed at band and broadcast methods at the rate of 140 kg ha-1 mixed with biochar at the rate of 25 and 50 t ha-1. The data collected on various parameters were statistically analyzed and planned mean comparisons were carried out to explain significant differences. Seeding depth significantly affected all the studied parameters except sugar sucrose. Sugar beet planted at 2 cm depth showed highest germination process and gave better yield production. Mean maximum ...

Shahid Ali1, Habib Akbar2, Mohammad Tariq Jan2, Mohammad Jamal Khan3 and Abdul Bari4

...ions and setts placement methods on the attributes of sugarcane were assessed at Sugar Crops Research Institute, Mardan-Pakistan. The experiment was conducted in RCB Design with split plot arrangement, over years (2012-13 and 2013-14). Planting sources i.e. standing (fresh canes frost exposed) trenched (stored canes frost protected) and canes portions top, middle, bottom and mixed 33 % each were assigned to main plots, while setts placement

Monireh Ahmadi Bani1*, Mostafa Kamali2, Iman Borji3, Soheyla Aslani3, Francis Fatoye4

...based on the old and new methods. Paired sample t-test was used for comparison between two methods and different conditions (eyes open and eyes closed). Traditional method for stability analysis showed no difference between two conditions in one minute standing (p ≥ 0.05). However, the new method confirmed that stability parameters significantly decreased in five minutes standing (p<0.001). Moreover, patients were unst...

Ali Mohammadi, Mohammad Hossein Ebrahimi

...align: justify;">Various methods of treatment have been used for patients suffering from knee osteoarthritis (OA). Among them total knee artroplasty is being practised frequently. This method is suitable especially for elderly subjects with limited degree of physical activity. However, most of young subjects are not recommended to be treated with this method owing to high level of physical activities. KineSpring knee implant system was desi...

Abiodun D. Olabode

...ield and farm management methods adopted in this area. Results were presented using charts and tables. The study revealed that; chemical fertilizers, manual weeding and shifting cultivation are essential for rice cultivation in the study area; the regression model established strong influence of farm-size at R-value of 0.64 on the yield of rice in this area; while estimated yields of 101.6tons and 57.9tons from irrigated and non-irrigated field respectively es...

Yongfeng Li, Mo Zhou, Xiao Wang, Libao Xie, Hua-Ji Qiu*

... as improving diagnostic methods and vaccines for classical swine fever.

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Amtul Jamil Sami1*, Madeeha Khalid1, Sara Iqbal1, Maira Afzal1 and A.R. Shakoori2

...is beneficial over other methods that employ toxic polymers or mineral coagulants.

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Atul Sharma, Aruna Chandra Singh, Gautam Bacher, Sunil Bhand

...ion, reported analytical methods, advancement in biosensing platform especially regarding electrochemical transduction are discussed in detail. Finally, future prospects toward the development of selective and sensitive aptasensors for antibiotic detection are presented.

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 Ali Murad Rahoo1,2, Tariq Mukhtar3,*, Simon R. Gowen1, Rehana Kanwal Rahoo4 and Shaukat Ibrahim Abro5

...ence of IJ by using both methods was non-significant. Similarly, with an increase in inoculum dose and time, invasion of host was significantly increased. There was little invasion at the 800 IJ dose even after 4 days whereas with the commercial dose (8,000), nematodes did migrate to the larvae and caused some infection after 2, 3 and 4 days. Greatest invasion took place with the highest dose (80,000) with 8 IJ successfully finding and penetrating the larvae. ...

Abdur Rahim1, Ghulam Abbas1, Muhammad Naeem2, Sara Ferrando3, Lorenzo Gallus3, Noor Khan4, Muhammad Hafeez-ur-Rehman4, Abdul Ghaffar5 and Abdul Mateen6

...ssing units, traditional methods were applied causing quality problem. Finally, it was concluded that quality of fish meal and production in our region is suitable for fish feed formulation. But the processing technique should be improved to match international standard, and to avoid declining in production of fish meal strategic management of fisheries resources will be required.

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Sezgi Arman* and Sema Isisag Ucuncu

... with different staining methods of alcian blue (AB) at pH 2.5, aldehyde fuchsin (AF), periodic acid-Schiff (PAS), KOH/PAS and PAS/AB (pH 2.5) and investigated. Goblet cells of anterior intestine were colored strongly by AB (pH 2.5), AF and PAS; and moderately by PAS/AB (pH 2.5) (AB dominant). In the mid part of the gastrointestinal tract, goblet cells were also stained strongly with AB, PAS and PAS/AB (pH 2.5) (AB dominant), however, their reactions to KOH/PA...

Sezgi Arman* and Sema Isisag Ucuncu

... with different staining methods of alcian blue (AB) at pH 2.5, aldehyde fuchsin (AF), periodic acid-Schiff (PAS), KOH/PAS and PAS/AB (pH 2.5) and investigated. Goblet cells of anterior intestine were colored strongly by AB (pH 2.5), AF and PAS; and moderately by PAS/AB (pH 2.5) (AB dominant). In the mid part of the gastrointestinal tract, goblet cells were also stained strongly with AB, PAS and PAS/AB (pH 2.5) (AB dominant), however, their reactions to KOH/PA...
Yusuf Koc1, Ecevit Eyduran1* and Omer Akbulut2
...and CART regression tree methods for different combinations of parent node: child node in the data set regarding animal science. To achieve the aim, 1884 Mengali lambs were provided for predicting weaning weight from sex (male and female), birth type (single and twin), birth year (2005, 2006, 2007, 2008 and 2009), farm (Research station, Mastung, Quetta, and Noshki), birth weight, dam age, and dam weight. To choice the best regression tree method, goodness of ...
Eman Mahmoud El-Diasty1,Madeha Abd El-Halim Ibrahimand Ghada Kamal El Khalafawy2,*
...apings). Phenotype-based methods for identifying yeast especially Candida species are often difficult, time-consuming and not enough to identify most yeast species. . Molecular biological techniques provide a useful alternative approach. Concerning with genotypic identification, polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) was applied for identification of Candida species, Cryptococcus albidus, Saccharomyces, Trichosporn, Torul...
Irshad Ahmad
...l and biological control methods for the substantial control of walnut borer. The present study offers valuable information through IPM, which appears to be a promising approach for the control of walnut borer. Collectively this strategy has resulted in significant reduction of the pest population in the target area of district Dir, Pakistan.
...
Sadaf Ijaz Malik1, Kiran Afshan2* and Mazhar Qayyum1
... traditional microscopic methods on the basis of standardized measurements. Amphistomes were flattened and stained in Borax carmine, some were sectioned in the median sagittal plane and histological slides of the worms were prepared for detailed morphological studies. Bile tissues, aspirates and blood samples of the same animals were analysed. The infection was found prevalent throughout the year but during post monsoon season it was relatively high. Morpholog...
Abdus-Subhan1, Qudratullah Khan1, Muhammad Mansoor2, Muhammad Jamil Khan1, Ammanullah2, Matiullah Khan3
...erization (P) and sowing methods (S) on Water Use Efficiency (WUE), wheat yield and economics, a field experiment was carried out at Arid Zone Research Center, Dera Ismail Khan, Khyber Pakhtunkhwah Province, Pakistan, during rabi season of 2014-15 and 2015-16. The experiment was laid in randomized complete block (RCB) design with split plot arrangement keeping pulverization viz. single tiller P1, double tiller P2, triple tiller P3

Nirbhay Kushwaha, Achuit K Singh, Brotati Chattopadhyay and Supriya Chakraborty

Recent advances in geminivirus detection and future perspectives
...ry ago.Agreat variety of methods have been developed that permit differentiation of viral pathogens.These methods, initially based solely on identifying the distinct biological characteristics of different viruses, were soon supplemented with methods based on light or electron microscopy and serology and subsequently by Enzyme Linked Immunosorbant Assay (ELISA) and finally the use of molec...

B. Ramanujam, R. D. Prasad, S. Sriram and R. Rangeswaran

Mass production, formulation, quality control and delivery of Trichoderma for plant disease management
...ops. There are two major methods of inoculum production of Trichoderma spp. viz., solid state fermentation and liquid state fermentation. In solid fermentation, the fungus is grown on various cereal grains, agricultural wastes and byproducts. The solid state production is highly labour intensive and fit for cottage industry. These products are used mainly for direct soil application in nurseries/main fields to suppress the soil-borne inoculum. In liquid state ...

M. S. A. Mamun and M. Ahmed

Integrated pest management in tea: prospects and future strategies in Bangladesh
...paration and application methods of new botanicals and microbial pesticide formulations, evaluation of field bio-efficacy and conservation of biological agents, cultural control measures, genetic techniques and sex pheromones technology. In tea husbandry, cultural control measures such as plucking, pruning, shade regulation, field sanitation, fertilizer application, manipulation or destruction of alternate hosts and selection of pest resistant/tolerant varieti...

Dipak Mandal, Paramita Bhowmik, Pronobesh Halder and M. L. Chatterjee

Determination of relative toxicity and base line data of dif-ferent insecticides against cotton mealybug (Phenacoccus solenopsis Tinsley)
...ated food treated insect methods were followed. The lowest LC50 values through treated food untreated insect method were recorded in chlorpyriphos 20 EC (226 ppm) and dichlorvos 75 EC (282 ppm) in treated food treated insect method, which was 8.89 and 7.13 times more relatively toxic in respective method after 24 hours when compared to the standard check flonicamid 50 WG. Based on the LC50 values (ppm) and relative toxicity, the descending order of toxicity wa...

Partha Pratim Ghosh and 2N. C. Mandal 

Some disease management practices for bacterial wilt of potato
Bao-Ming Ge* Dai-Zhen Zhang, Qiu-Ning Liu, Sen-Hao Jiang, Jun Cui, Chun-Lin Zhou and Bo-Ping Tang
...;s patchiness regression methods in each season (P< 0.001). The abundances were markedly affected by habitat type (F5,120 = 49.409, P < 0.001) and season (F3,120 = 13.577, P < 0.001), however, there was no significant interaction of habitat type and season (F15,120 = 0.529, P = 0.919). The abundances distribution was significantly different among habitats in each season as shown by one-way ANOVA, the higher abundance c...
Fariha Imtiaz1, Muhammad Islam1, Hamid Saeed2,*, Bushra Saleem1, Maryam Asghar1 and Zikria Saleem2
... hot and cold extraction methods. Phytochemical analysis of leaves was performed on powder and extracts of leaves using already reported methods. Mineral analysis was done using atomic absorption spectrophotometer. Hair growth promoting effects were examined using alopecia mouse model. Phytochemical analysis demonstrated the presence of higherquantities of carbohydrates, proteins and secondary metabolites in methanol compare...

Shah Saud Ahmad and Muhammad Zafarullah Khan*

...hnology (94%) and sowing methods (93%) learned through FSC. Mass contact method was rarely used in the study area as only 10% of the respondents ranked it very high and 28% respondents reported it very low on likert scale. It can be concluded that FSC had developed the linkages of the respondents with agriculture research and water management department in the study area. Top learned skills from this approach were fertilizer application, improved agricultural ...
Ghulam Abbas1,Asif Nadeem1,*, Masroor Ellahi Babar2, Tanveer Hussain2, Muhammad Sajid Tahir3, Wasim Shehzad1, Rajput Zahid Iqbal3, Muhammad Tayyab1 and Maryam Javed1
...ral resources. Molecular methods have an important role in phylogeny and diversity analysis. In Pakistan hog deer (Axis porcinus) is an important wild species belongs to the family Cervidae. The hog deer is one of the least studied species at molecular level in Pakistan. The present study was planned to investigate its molecular phylogeny and diversity analysisusing mitochondrial cytochrome b, cytochrome c and d-loop region. Samples...

Sania Shaheen1*, Hina Fatima2 and Muhammad Azeem Khan3

...stify;">In Pakistan, two methods mainly using for the transplanting of rice paddle (Conventional method) and Direct seeded method (Dry method) in rice growing areas of Punjab. In last some years, the direct seeded rice system is introduced in some of the rice cropping districts of Pakistan. The current research estimate the technical efficiency of conventional and dry rice farmers and also determine the factors which significantly contribute to increase the ri...

Raza Ullah1*, Qaisar Shah Safi2, Ghaffar Ali3 and Irfan Ullah

...actices, proper time and methods of harvesting, packaging, storage and processing. There are various middlemen (market intermediaries) involved at different stages in the marketing of citrus. This study is an attempt to examine the marketing margins of the intermediaries involve in the value chain of sweet oranges of citrus group. Three villages in Bunir district are randomly selected and a total of 120 sampled respondents are selected using proportional alloc...
Kadir Karakuş1, Bahat Comba2, Abuzer Taş3, Tunahan Sancak3, Arzu Comba2, Devrim Saripinar Aksu4, Hasan Koyun5 and Mohammad Masood Tariq6,*
... of different castration methods in 4-month-old male Norduz sheep resulted in changes in the blood hormone levels, it was determined that these changes did not affect body weight. In conclusion, the evaluation of the process in castrated six-month-old sheep revealed an increase in the body weight which was similar to that in the control group.
...
Omar S.O. Amer1,3,*, Mohamed I. Waly2,4 andSaeed A. Al-Zahrani1
...ng different statistical methods to develop effective prevention and control strategies.
...
Lu Liu1, Sher Khan Panhwar2, Tianxiang Gao3, Zhiqiang Han3, Chunhou Li4, Dianrong Sun4 and Na Song1,*
...the species by molecular methods, we sequenced a fragment of the cytochrome oxidase subunit I (COI) gene of mitochondrial DNA of Liza affinis and Liza klunzingeri from coastal waters of China and Pakistan, respectively. Sequences of L. carinata were obtained from GenBank. A neighbor-joining tree showed the specimens to form a strong monophyletic group. The mean within-species genetic distances for L. affinis, L. carinata, and...
Mohammad Umar Farooq1*, Sarfaraz Ahmad2, Ghulam Nabi1, Ijaz Ali1 and Imtiaz Ahmad1
... m 2 quadrate methods, weighed and then oven dried at 60 oC to find out the dry weight for above pytomass carbon Mg C ha-1 (Brown and Lugo, 1982). Similarly, the same 1 m2 quadrate area was dug up to the depth of root zone for each grass and the root portion was separated, weighed, oven dried at 60 Co for estimating below ground pytomass carbon Mg C ha-1. Carbon pool in above ground phtyomass wa...
Shakeel Ahmad Anjum, Muhammad Farrukh Saleem, Muhammad Shahid, Abdul Shakoor*, Muhammad Safeer, Imran Khan, Ayesha Farooq, Iftkhar Ali and Usman Nazir
...e objectives to optimize methods of B and Zn application in maize and ultimately enhance profitability. The experiment was laid out in randomized complete block design with factorial arrangement during autumn 2015. Treatments were replicated three times. The treatments were comprised of C = no Zn and B (Control); Zn (S) = soil application of Zn @ of 12 kg ha-1; B (S): soil application of B @ 3 kg ha-1; Zn (F) = foliar application of 1% Zn...
Faiz Muhammad1,*, Syed Khurram Fareed1, Urooj Zafar1, Taseer Ahmed Khan2 and Aqeel Ahmad1
...nt DNA crude preparation methods were used and broth dilution method found simpler and most efficient for positive PCR. Primers were selected for 16s rRNA and it could detect up to 100 cfu and 250 cfu from MS and MG respectively in a samples. We have tested pure DNA of other mycoplasmas (5 species from avian origin and 3 other mycoplasmas species) but it produced only the genus specific band. The optimized ratio of tPCR primers were 1: 1: 10: 1. For Outer F, I...

Sikandar Hayat, Khalid Usman* and Muhammad Tahir Amin 

...N fertilizer application methods (broadcast and placement) were kept in subplots. Results revealed that row spacing of 30 cm had higher yield and yield components besides higher benefit cost ratio (3.50). Nitrogen placement method proved to be superior to broadcast method of application regarding wheat yield and yield contributing traits. Row spacing and N application methods interaction showed that zero tillage wheat had th...
Mubashir Hussain1,Safdar Ali1, Muhammad Naveed Tahir1, Ghulam Abbas Shah1, Ijaz Ahmad2*, Muhammad Aqeel Sarwar3 and Sohail Latif4
... of seed bank extraction methods revealed higher weed seeds density, weed frequency with more diversity of weed species under sieving method in comparison to seedling emergence method. Therefore, sieving method was considered superior over seedling emergence method. The feasibility analysis of seed bank extraction methods indicated that sieving was cost-effective, less time consuming, more user friendly with higher accuracy ...
Muhammad Qaisar Nawaz
...d conditions. Two sowing methods i.e. broad cast and drill sowing with 30 cm apart rows and four nitrogen levels (75,100,125 and 150 % of N recommended dose i.e. 150kg ha-) were tested. Recommended dose of PK fertilizer (85-60 PK kg ha-1) was used uniformly with experimental N rates. Data on plant height (132.00 cm), number of plants (91.33 m-2), number of tillers (146.00 m-2), number of leaves tillers-1 (...
Semra Benzer1,* and Recep Benzer2
...mation result of the two methods which are Length - Weight Relations and Artificial Neural Networks from Uluabat Lake regarding narrow-clawed crayfish (Astacus leptodactylus Eschscholtz 1823) between 2015 and 2016. The relationships between total length (TL) and total weight (TW); carapace length (CL) for Astacus leptodactylus caught from Uluabat Lake were presented with tradional method of length-weight relation (LWR) Equation and Artificial Neu...
Temel Gokturk1, Elif Tozlu2,* and Recep Kotan2
...ntal issues, alternative methods were sought in the past; and in this context, studies were conducted to find new methods in which fungi and bacteria were used in the biological control against pests. Totally 10 bacterial strains including 2 strains of Brevibacillus brevis (CP-1, FD-1), 1 strain of Bacillus thuringiensis (FDP-1), 2 strains of Bacillus thuringiensis subsp. kenyae (FDP-8, FDP-42), 2...
Falah Baiee1,3, Wahid Haron1,*, Rosnina Yusoff1, Ariff Omar,2 Nurhusien Yimer1, Salman Hammadi1, Tarig Ahmedeltayeb1, Asmatullah Kaka4
...ion was the same in both methods. Also, there were no significant differences in semen parameters such as sperm volume, motility, morphology, viability, and concentration. In conclusion, gradation of electrical stimulation into three stages (our modified Method II) could help to ease the collection of semen samples from bulls with minimum discomfort signs. Furthermore, the modified method is also recommended to use for other animals, in particular, the wild an...
Shumaila Ashfaq1, Liaqat Ali2*, Muhammad Ashraf Zia2, Rizwan Ahmad Khan2 and Mehtash Butt3
...veral factor and various methods have been used to reduce and treat the pain of PDPH. We evaluated effects of hydrocortisone on the treatment of PDPH in obstetrical patient.To compare mean decrease in Visual Analogue Scalepain score in patients who developed PDPH after elective caesarean section under spinal anaesthesia who were given conventional treatment versus conventional treatment plus hydrocortisone.Double blind randomized control trial. Study conducted...
Wali Khan1,*, Noor-Un-Nisa2 and Aly Khan3
... smear and concentration methods. One hundred and fifteen (11.04%) participants were found infected with one or more than one intestinal protozoans. Forty one (35.6%) of the participants were infected with single parasite and seventy four (64.3%) with multiple infections. Entamoeba histolytica 30.5% (n=77/252), Giardia lamblia 15.0% (n=38/252), Ascaris lumbricoides 17% (n=43/252), Trichuris trichura 11.1% (n=28/252), Enterobius v...
Saleh S. Alhewairini
...s on finding appropriate methods for managing and controlling it. A huge fund was given for managing and controlling RPW in several countries. In this study, the potential toxicity of oxamyl to RPW was evaluated under both laboratory and field conditions. In the laboratory, oxamyl had significant effects on the mortality of RPW. After exposure to direct spray of oxamyl, adult mortality was 62, 82 and 100% whereas larvae mortality was 72, 77 and 100% after 1, 2...

Hafiz Muhammad Tahir1, Khanum Zahra2, Arooj Zaheer2, Khizar Samiullah3

...ng to introduce advanced methods to manufacture spider silk artificially to enhance silk production. Silk fiber can be designed after determining type and sequence of constituent amino acid or by inserting silk producing gene in another suitable animal.

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Niamat Ullah Khan1*, Abdul Aziz Khakwani2, Sami Ullah2, Najeeb Ullah2, Abdur Rauf3 and Inam Ullah2

...plicated thrice. Tillage methods were allotted to main plots while nitrogen levels were kept in sub plots. Results showed that RT produced higher bolls per plant, weight per bolls, lint yields, earliness % and benefit cost ratio (BCR) than FT. Ridge tillage gave lower percent of lodging. Results for nitrogen indicated that bolls numbers per plant, bolls weight, lint yield, earliness, lodging, BCR, fibre length and fineness were highest at 150-200 kg N/hectare....
Amjad Khan1,*, Muhammad Hassan Mushtaq1, Mansur ud Din Ahmad1, Jawad Nazir1, Zahida Fatima2, Asghar Khan3 and Shahid Hussain Farooqi3
...it and HI as serological methods for diagnosis, we found a 24.13% (168/696) overall seroprevalence, where the seroprevalence for H3N8 was 14.51% (101/696), for H7N7 6.03% (42/696), and 3.59% (25/696) as mixed infections, suggesting EIV current and active circulation in equine population of Pakistan. Statistical analysis suggested predicting variables including “local equine density per 2 KM”, “equines workings in ponds and rivers”, and ...
Wali Khan1,*, Noor-Un-Nisa2 and Muhammad Asif Nawaz3
... smear and concentration methods. Two hundred and twenty one (21.2%) participants were found infected with one or more than one intestinal tapeworms. Seventy seven (7.39%) of the participants were infected with single parasite and one hundred forty four (13.8%) with multiple infections. Taenia saginata 32.6% (n=146/447), Ascaris lumbricoides 20.3% (n=91/447), Hymenolepis nana 19.7% (n=88/447), Trichuris trichura 14.3% (n=64/447),

Saeeda Raza, Muhammad Naeem Safdar, Amer Mumtaz, Nouman Siddiqui, Khalid Nasim and Muhammad Amjad*

...s utilizing home cooking methods and evaluate the sensory attributes and shelf life at ambient conditions. Four different recipes of baby foods were developed containing 50% cereals, 30% legumes, 10% vegetables and 10% nuts and seeds. Different processing methods cleaning, washing, germination of pulses, peeling, cutting and blanching of vegetables, drying, roasting, grinding and mixing of ingredients were used. Recipe &ldqu...

 Abdullah Adil Ansari* and Kumar Sukhraj**

...rather than conventional methods based on the earlier observations.

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 Zaheeruddin Mirani*and Aslam Memon**

...and method demonstration methods of technology transfer were perceived as effective methods of technology transfer. Farmers were able to increase the yields of their farm produce. However, the picture says that there is yield gap between the potential yield obtained by research scientists and yield obtained by the farmers. Farmers were of the opinion that both public and private extension should increase their timely visits....
Mustafa Sahin1,*, Esra Yavuz1 and Fatih Uckardes2
...ponents Regression (PCR) methods. For this reason, eggs were collected from 20 to 24 weeks of age, non-selected and random-mated Japanese quails. In this study, we investigated the characteristics of egg external quality such as egg width (X1), egg length (X2), Haugh unit (X3), and shape index (X4). The impact of these variables on albumen index was analyzed. Due to the multicollinearity between independent variables...

 Saima Rani and Irum Raza*

COMPARISON OF TREND ANALYSIS AND DOUBLE EXPONENTIAL SMOOTHING METHODS FOR PRICE ESTIMATION OF MAJOR PULSES IN PAKISTAN
...mong the two forecasting methods namely trend analysis and double exponential smoothing. Measures of accuracy (MAPE, MAD and MSD) were used as the model selection criteria that could best describe the trend of prices of major pulses such as gram, mash, masoor and mung during 1975-76 and 2009-10. Double exponential smoothing method was found to be pertinent for price estimation of major pulses in Pakistan because of smaller values of accuracy measures. Six-year...

 A.A. Mengal* , M. U. Mallah, Z. A. Mirani* and B. N. Siddiqui**

AN ANALYSIS OF PUBLIC AND PRIVATE AGRICULTURAL EXTENSION SERVICES IN BALOCHISTAN, PAKISTAN
...spectively, as preferred methods of extension. Most of the farmers agreed that public extension field staff provide information regarding application of irrigation and private extension field staff provided advice for the use of plant protection measures. Significant differences were observed between public and private extension field staff on various statements regarding competency level and agronomic practices.

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 Amir Khatam*, Sher Muhammad, and Ijaz Ashraf**

ROLE OF INDIVIDUAL CONTACT METHODS IN DISSEMINATION OF AGRICULTURAL TECHNOLOGIES
...ts of individual contact methods in diffusion of agricultural technologies from 280 randomly selected farmer respondents. Researchers collected the data with the help of a pretested and validated interview schedule using survey technique. The results show that majority of the farmer respondents is aware of the farm visits (66.43%), demonstrations being conducted (62.50%) and home visits made by the extension staff (60.71%) as extension

 Muhammad Sohail*, Imtiaz Hussain*, Riaz-ud-din*, Syed Haider Abbas*, Maqsood Qamar* and Muhammad Noman*

EFFECT OF SPLIT N FERTILIZER APPLICATION ON PHYSIOAGRONOMIC TRAITS OF WHEAT (TRITICUM AESTIVUM L.) UNDER RAINFED CONDITIONS
...ha and three application methods i.e. full basal N dose at planting and N application in two and three equal split doses at tiller formation and stem elongation stages. Maximum grain yield -1 -1 (5.20 t ha ) was achieved when N was applied @120 kg ha in three equal split doses at planting, tiller formation and stem elongation stages. N application in 2 and 3 split doses resulted in 25 - 50% grain yield advantage at all N rates as compared to single basal N dos...

 Sajida Taj*, Akhter Ali*, Nadeem Akmal*, Shujaat Yaqoob** and Mubarik Ali***

RAISED BED TECHNOLOGY FOR WHEAT CROP IN IRRIGATED AREAS OF PUNJAB, PAKISTAN
...s also one of the modern methods of planting crop with significant water saving. The study was planned and conducted by the Social Sciences Research Institute, Faisalabad in 2011-12 to assess the determinants of the adoption of the raised bed technology for wheat crop in irrigated Punjab, Pakistan. The study was conducted at three sites of the districts Faisalabad and Toba Tek Singh where the Water Management Research Institute, University of Faisalabad promot...

 Muhammad Shahzad*, Akhter Ali**, Abdul Hayee Qureshi**, Naveed Jehan*, Imran Ullah* and Majid Khan*

ASSESSMENT OF POST-HARVEST LOSSES OF PLUM IN SWAT, PAKISTAN
...ons were poor harvesting methods, over maturity, insufficient cold storage facility, poor grading, low-quality packaging materials and poor infrastructure. Total post harvest losses in the marketing channel of the plum were 21.51% of which 5.12% occurred at farm level, 1.44% at wholesale level, 6.31% at retail level, and 8.64% at the consumer level. Harvesting at proper maturity, using experienced labor and storage facility can reduce extent of post harvest lo...

 Amir Khatam*, Sher Muhammad**, Badar Naseem Siddiqui***, Muhammad Zakaria Yousuf Hassan****, Ijaz Ashraf**, Muhammad Zafarullah Khan***** and Aijaz Kuharoo******

COMMUNICATION OF AGRICULTURAL INFORMATION THROUGH GROUP CONTACT METHODS IN PAKISTAN
...on through group contact methods in Pakistan. Data were collected from 280 randomly selected farmers of four districts and analyzed using descriptive statistics. Survey method was used for data collection by researchers using a pre-tested research instrument. The results of the study show that sources of agricultural information used by the farmer respondents were seed/ fertilizer dealers, workshops, panel discussions, role playing and brainstorming. However, ...

 Aniqa Iram*, Javed Khan**, Nadeem Aslam**, Ehsan-ul-Haq**, Habib Iqbal Javed**, Muhammad Irfan*, Awais Rasool*, Muhammad Ishaque Mastoi** and Sumera Aslam* 

EFFICACY OF PLANT DERIVED OILS AND EXTRACTS AGAINST WHITEFLY, BEMISIA TABACI (GENNADIUS) ON SESAME CROP
...nts worldwide. Different methods are being used for its control. The present experiment was conducted to determine the effect of some plant extracts of mint (Mentha spp.) and geranium (Pelargonium graveolens) and soybean oil (Glycine max), mustard oil (Brassica spp.) and taramera oil (Eruca sativa) against whitefly, Bemisia tabaci on sesame crop. The data were recorded 24h before and 24h, 48h, 72h and 168h after application of each spray material. The results ...

 Syeda Aimen Hadi*, Abid Ghafoor Chaudhry*, Aftab Ahmed** and Shaheer Ellahi Khan***

PERCEPTION, APPROACHES AND PRACTICES OF LOCAL FARMING AND DEVELOPMENT: AN ANTHROPOLOGICAL APPROACH
... nexus of modern farming methods, due to which they are being abandoned by the farmers. Modern farming which is representative of development has increased the competition, transitioning, breaking the community into people experimenting on modern technology and those who are still finding means to adapt their traditional agriculture to the fast changing needs of the societies. The research was carried out in the villages of Ghora Gali and Aruka through qualita...

 Muhammad Asif Jamil*, Nowshad Khan*, FarhatUllah Khan* and Muhammad Zakria*

EFFECTIVENESS OF AGRICULTURAL EXTENSION SERVICES IN DISSEMINATION OF INFORMATION TO THE FARMING COMMUNITY OF DISTRICT BHIMBER, AZAD JAMMU AND KASHMIR
... training were effective methods of communication. The study concluded that other sources of information i.e., relatives, friends were more influential in bringing changes in the behavior of the respondents and solving the problems of farm related issues. It was found that 38% of the aware respondents yield was 50% greater than unaware farmers. It was also concluded that 80% of the respondents had increased the production of wheat while 80.67% had increased th...

 Abdul Razaq*, Muhammad Jamal Khan*, Tahir Sarwar* and Mohammad Jamal Khan**

EFFECT OF DEFICIT IRRIGATION, SOWING METHODS AND MULCHING ON WHEAT YIELD AND NITROGEN UPTAKE
...ere studied i.e., sowing methods, mulching and deficit irrigation with four levels viz: full irrigation (DI ), 20% deficit irrigation (DI ), 40% deficit irrigation (DI ) and 60% deficit irrigation (DI ). Soil moisture was continuously monitored and full irrigation was determined on the basis of 50% Management Allowed Deficit (MAD). Results indicated that sowing methods, mulching and various levels of deficit irrigation signi...

 Ehsanullah*, Muhammad Amjad Shahzad*, Shakeel Ahmad Anjum*, Ali Zohaib*, Muhammad Ishfaq* and Ejaz Ahmad Warraich*

EFFECT OF DIFFERENT SOWING METHODS AND PLANTING DENSITIES ON GROWTH, YIELD, FIBER QUALITY AND ECONOMIC EFFICACY OF COTTON
...r under different sowing methods and planting densities. A field experiment was conducted to evaluate the influence of different sowing methods and planting densities on growth, yield, quality and economic returns of cotton. Sowing methods included pit planting (1 m × 1 m pits), bed planting (75 cm apart beds), ridge planting (75 cm apart ridges) and line sowing with varied inter row...

Hina Zubair1, Saima Perveen2, Saeed Alam3, Akkad Rafiq4* and Sara Ejaz 

...ifferent family planning methods in relation with socio-demographic factors at divisional headquarter hospital in a year time. A retrospective study was performed by analyzing one year records from family planning clinic at divisional headquarter hospital. At family planning centers, a total of 920 individuals attended the Reproductive Health Services from April 2015 to March 2016. Among them, 92% individual utilized temporary methods<...
Burcu Onuk1,*, Murat Kabak1, Bünyamin Sahin2 and Nazan Gezer İnce3

 

...ple of the stereological methods. The volume and volume fractions of structures were assessed. Results obtained from the CT images were compared between stork and seagull. The volume fractions of the conchae, nasal cavity and nasal septum were 30.24%, 55.22%, 14.54%, and 33.03%, 53.23%, 13.73%, in stork and seagulls, respectively. The volume fractions of structures did not show statistical difference between two species. Our findings suggest that the nasal str...
Abdul Nabi Jatt1,*, Sarfraz Ali Tunio1, Shaista Bano Memon1, Abdul Sattar Qureshi2 and Muhammad Aqeel Bhutto
...ive to recent biological methods. This method could be applied for the identification of various enzymes on routine basis directly in bacterial cultures and biological fluids.
...
Grace Chinenye Onyishi, Ifeanyi Oscar Aguzie, Joseph O. Okoro, Christopher Didigwu Nwani*, Ngozi Ezenwaji, Ndubisi Stanley Oluah and Fabian C. Okafor
...ities. Light and teasing methods were used for recovery of parasites from the snails. A total of 618 snails belonging to 7 species (Achatina achatina, A. belteata, A. degneri, Limicolaria aurora, L. flammea, Lamellaxis gracilis and Orthalicus sp.) were collected. A. achatina was the most abundant species (35.6%) while A. degneri was the least (1.3%). Overall, 388 snails (62.8%) were infected. A. achatina had the highest (76.4...

Ejaz Ashraf1*, Hafiz Khuram Sharjeel1, Raheel Babar2, Muhammad Junaid1, Qamar Iqbal3, Rabia Rasheed1 and Nosheen Fatima1 

...rogram and demonstration methods were dominant approaches used in technology transfer. The mean of the results showed that low allocation of budget, massive operational area to give satisfactory coverage, less use of multimedia, unavailability of training facilities to EFS, manifold duties assigned to extension officers and top-down dimension of planning of implementation of extension approaches were main weaknesses. The steps like effective evaluation of exte...
Yan Li1, Kai Zhan1,*, Junying Li1, Wei Liu1, Ruiyu Ma1, Shengnan Liu1, Tao Han2, Shaoquan Li2, Shaolin Wang2 and Yang Hu2
...de to choice of breeding methods for poultry enterprises.
...

Osama Elshazly1, AbdelSatar Arafa1, Mohammed A. Rohaim2, Ismaeil M. Reda2 and Hussein A. Hussein2*

... and molecular detection methods. Full-length hemagglutinin (HA) sequences of six representative H5N1 isolates were analysed to study their genetic evolution followed by estimation of their evolutionary rates among different virus clusters. This analysis revealed a high evolution rate for clade 2.2.1.2. Additionally, analysis of selection pressures in the HA gene revealed a positive selection pressure. Deduced amino acid analysis revealed characteristic mutati...
Waqas Ali1,*, Arshad Javid1, Syed Mohsin Bhukhari1, Ali Hussain1, Syed Makhdoom Hussain2, Hira Rafique1
...tive effects of sampling methods used on habitat and taxa under consideration. In addition, study area must be sub-divided into small patches and more than two suitable sampling techniques should be used to record all possible species.
...
Jiji Li*, Tianyang Zu, Xiangli Dong, Xiao Yang, Wei Liu and Changwen Wu
...gs could help to develop methods of disease resistance in the aquaculture industry.
...
Niamatullah Kakar, Farhat Abbas, Muhammad Shafee and Tauseef Asmat*
...st while other detection methods were not that sensitive to detect the tuberculosis at that stage. Two patients were reported with history of tuberculosis but were effectively treated before imprisonment. On age basis, 128/186 (68.88%) patients were of younger age (18-30 years) and 10 (5.37 %) were above 50 year. Illiteracy, unemployment (daily wages) and tobacco smoking were some of the main risk factors common in inmates. The trend of sharing blankets, towel...
Baoying Guo*1, Yu Chen1, Chuan Zhang2, Zhenming Lv1, Kaida Xu3, Hongling Ping4 and Huilai Shi4
... likelihood and Bayesian methods were used to build phylogenetic trees based on protein coding mtDNA genes of 37 cuttlefish species. S. lycidas have a close relationship with S. pharaonis, S. aculeata, and S. esculenta. This result confirmed the relationships of S. lycidas as being similar to the traditional taxonomy. This study plays an important role in the investigation of phylogenetic relationships, taxonomic resolution, ...
Mingming Ning1,2, Yanjie Zheng1, Yuanyuan Dun1, Weijun Guan2 and Xiuxia Li1,*
...he isolation and culture methods of chicken amniotic fluid stem cells (CAFSCs) in vitro, characterizing CAFSCs and inducing CAFSCs to differentiate into three layers. Methods and results include that more CAFSCs are gained via thermoelectric method with lower cost. Groping and optimizing culture system abiding by the idea of simulating the chicken body environment, involving adding proportion of chicken serum and some...
Danyu Zhang1, Ying Liu1, Qinxin Shi1, Zhiwei Peng1, Yan Hua1 and Zhijun Hou1,2,*
...ting and next sequencing methods. The parasites were Capillaria sp., Baylisascaris devosi, Echinochasmus sp., and two Coccidianspecies. The Echinochasmus sp. andtwo Coccidian species were first time found either in sable or in other martens. The 434 genus of bacteria, belong to 30 phylum, the more popular bacteria were Proteobacteria (33.54%), following by Firmicutes (18.58), Acidobacteria (12.82%)...
Sadaf Noveen1*, Shaista Habib Ullah2, Babar Alam3
Nasreen Ishaq1, Arif Rasheed Malik2, Zameer Ahmad1, Saad Ehsan Ullah3
...r detection of different methods of identification to establish a baseline of identity e.g. dental data, fingerprinting, DNA analysis, anthropometry, identification of sex, assessment of age, determination of height and blood groups identification. Among these, DNA analysis and dental data provide easiest identifications, however, these techniques are expensive and not readily available necessitating additional techniques for identification. One of such novel ...
Masood Nizam Tabassum1*, Muhammad Ather Khan2, Saira Afzal3, Amir Gilani4, Abdul Wahab Gureja5, ShafaqTabassum6
Yan Li1, Kai Zhan1,*, Junying Li1, Wei Liu1, Ruiyu Ma1, Shengnan Liu1, Haijun Cui1 and Xin Zhang2
...s. Preferential trimming methods were the removal of the first and third or second and fourth toes of day-old chicks in Hy-Line strain.
...

Saeed Ullah1, Amanullah Jan1, Murad Ali2*, Wiqar Ahmad3, Hafeez Ur Rehman2, Muhammad Ishaq2 and Bilal Ahmad2  

...utrients and application methods showed that application of nutrients with band placement method gave excellent results even at lower doses emphasizing proper application method as more important than mare increasing nutrient levels for obtaining higher returns 

...
Muhammad Shaheer1*, Asad Aslam Khan2, Nasir Ahmed3, Tehseen Mahmood Mahju4, Ummarah Rasheed5
Taimoor Akram Khan1*, Anam Farooq2, Amna Farooq3, Muhammad Nasir1, Gohar Khan4 and Ali Akram Khan1
Sahar Naz1, Farhan Rasheed2, Muhammad Saeed3*, Shagufta Iram4 and Ambereen Anwar Imran5
Munir Ahmad1, Aisha Hameed2*, Saba Khaliq3, Hamid Saeed Malik4 and Shahida Mohsin5
Faisal Masood*1, Ranjit Kumar Sah2 and Ahmad Humayun Sarfaraz3
Naveed Rashid*1, Muhammad Aamer2, Uzma Malik3, Nimra Arif4, Muhammad Waqar Akram5 and Ayesha Irshad6
Muhammad Younus1*, Sabah Usman2 and Samia Jawed3

Muhammad Khalid1,2 and Muhammad Naeem2* 

...don idella. Standardized methods were used to determine the proximate composition of 72 samples. On wet weight basis, mean percentage for water was 80.76 %, ash 3.40 %, 4.31 % for fat and 11.53 % for protein in C. idella. Percent water contents showed inverse relationship with % protein (dry and wet body weight), % ash and % organic contents (wet body weight). Wet body weight (W) showed negative correlation (P < 0.001) with percent water, percent fat (dry a...

Ansaar Ahmed1, Imtiaz Hussain2*, Ibni Amin Khalil3, Subhanulla3, Gulzar Ahmed3, Imtiaz Ahmad3 and Muhammad Imtiaz2  

...ify;">Crop establishment methods like broadcasting of wheat and manual planting of maize on flat surface coupled with flood irrigation are used in irrigated maize–wheat system of Khyber Pakhtunkhwa (KP) province that requires skilled labor, intensive tillage and more water for irrigation. In this study, zero tillage and bed planting effect in comparison with farmer practice on maize-wheat system productivity was evaluated at five sites in district of Now...
Safia Rehman1, Nazish Mazhar Ali1,*, Gerald B. Pier2, Iram Liaqat1 and Bushra Mazhar1
...ment of chemotherapeutic methods against multidrug resistant bacterial pathogens in chronic and acute lung infections. It provides a practical approach towards the use of nanoparticles to enhance antimicrobial activity against these pathogens.
...
Arifa Mehreen1, Iram Liaqat2,Muhammad Arshad3, Muzzamil Waheed4 and Najma Arshad1,*
...biological and molecular methods. The agar disc diffusion method was used to evaluate the antibiotic susceptibility. Virulence-associated genes were detected using PCR while association analysis was used to test the likelihood of strains carrying combinations of genes involved in toxin production and/or host immune evasion. Highest resistance was observed against beta-lactam group followed by cephalosporin, lincosamide, tetracycline, macrolides and aminoglycos...

Ejaz Ashraf1*, Hafiz Khurram Shurjeel2 and Mujahid Iqbal3  

...or comparison of the two methods of instructions. The results of the study revealed that both methods were equally applicable and effective regarding timely transfer of information or knowledge to the farmers for aphid attack on wheat crop. Hence, it is concluded that the goal for creating awareness among farmers for the use of Mobile phone cellular technology is as effective as face-to-face (traditional method) for dissemin...
Rida Zainab1, Sana Elahi1, Afshan Kaleem2,*, Daniel C. Hoessli3, Mehwish Iqtedar2, Roheena Abdullah2, Faiza Saleem2, Shanza Khan1, Anusha Ijaz1, Shagufta Naz2 and Abdul R. Shakoori4,5,*
...One of the computational methods becoming increasingly popular to develop new drugs is relying on Pharmacophores. This method was utilized to develop pharmacophore models of Akt2 inhibitors and β2-Adrenoceptor agonists. A pharmacophore model is proposed, using fourteen second generation and one first generation antipsychotic drugs for Schizophrenia that are effective against both 5-HT2a and D2 receptors. Hydrogen bond acceptors (HBA), aromatic rings (AR r...

Mian Noor Hussain Asghar Ali, Liaquat Ali Jamali, Shakeel Ahmed Soomro*, Shakeel Hussain Chattha, Khalil Ahmed Ibupoto, Naseer Ahmed Abbasi and Noor Mehdi Qumi  

...days under two different methods i.e. one kept open and other covered with sugarcane trash. The samples of sugarcane were taken at an interval of 24 hours to determine different quality parameters i.e. cane weight, brix, pol/sucrose, purity, fiber and commercial cane sugar. The results revealed that weight loss and fibre content throughout increased with increasing storage period. However, a decrease was observed with increasing storage period for brix content...
Nimra Naseer, Adeela Fatima and Imran Sajid*
...ical screening different methods including cross streak, agar plug and well diffusion method were performed. Seven Streptomyces strains including NWMD-6, NWMD-7, NWMD-10, NWMD-11, NWMD-12, NWMD-15 and NWMD-16 were found to have significant antimicrobial activity against MDR bacterial pathogens. Secondary metabolite profiling of each methanolic extract by TLC and HPLC-UV signified the presence of unique and diverse metabolic compounds. The study predicts...

Aneel Salman1, Muhammad Iftikhar ul Husnain1, Inayatullah Jan2*, Muhammad Ashfaq2, Mudassar Rashid1 and Usman Shakoor1 

...ing, and drip irrigation methods are prevalent in the research area. The major hurdles that farmers perceived in adaptation are limited access to credit, lack of access to information, and institutional support. As expected, no significant variations were found across districts in the characteristics of respondents (age and income) that can affect their perceptions, their adaptation strategies, and obstacles faced in adaptation. The results of the study need t...

Asma Sohail1*, Aina Rafique2, Kashif Sarfraz Abbasi1 and Maryum Arif3 

...tivity. Effect of drying methods (sun-, oven- and freeze drying) on antioxidant and antimicrobial activities of date fruit powders from three varieties Zahidi, Aseel and Muzafati were investigated. The DPPH activity was significantly higher in Muzafati sundried date powder, whereas maximum phenolic and flavonoid contents were significantly higher in Muzafati freeze dried date powders. Results suggest that freeze dried date fruit powder had the highest potentia...

 Nazish Mazhar Ali1, Saiqa Andleeb2, Bushra Mazhar1, Anum Imtiaz1, Shaukat Ali2*

Assessment of antibiogram and resistogram of pathogenic bacteria isolated from irrigated wheat field water
... and agar well diffusion methods. Optimum temperature for growth of all bacterial strains was 37oC and pH 7 except Salmonella which had pH 6. Among all the antibiotics Oxacillin was less potent as three bacterial strains showed resistance against it while others antibiotics were more potent. Minimum inhibitory concentration of heavy metals for all bacterial isolates was different. It was concluded that irrigated pathogenic bacteria are highly resistant to heav...

 Abbas Ali1, Muhammad Altaf2*, Muhammad Samar Hussain Khan3

Winter survey of birds at Keti Bunder, district Thatha, Pakistan
...sing direct and indirect methods. During the survey, total of 49 winter season bird species belonging to 33 genera
and 21 families were recorded. A total of 4280 birds were recorded dedicated survey effort from the Keti Bunder.
Dominance (D), Shannon-Wiener diversity Index (H’), Simpson Index (S), Evenness (E) and Margalef (R) were
observed form the study area as; 0.06, 3.23, 0.94, 0.52 and 5.74 respectively. During the surveys noted...

 Raazia Kiran1, Alya Riaz1, Muhammad Irfan1*, Hafiz Abdullah Shakir2

An overview of pre-treatment methods used for bioconversion of lignocellulosic biomasses into valuable products
..., and bursting, chemical methods i.e.,
solvents, acids, salts, bases, the physicochemical processes include liquid hot
water and AFEX (ammonium fibre explosion), biological methods involving whiterot/
brown-rot fungi and bacteria, and quite a few combinations thereof to
breakdown the lignocellulose into its components. Pre-treatment process changes
cellulose morphological, chemical and phys...

Abid Muhammad Shah1, Safdar Ali1*, Ijaz Ahmad2, Gulzada Wazir1, Obaidullah Shafique1, Muhammad Amir Hanif1, Bashir Ahmad Khan3 and Sumaira Zareen4 

...nt herbicides and sowing methods on wheat productivity and population dynamics of weeds. Different wheat sowing methods (S) were Broadcasting, Line sowing, Ridge sowing, Bed sowing and herbicides used were Ally Max, Clean wave, Starane M. and Buctril Super 60 Ec. The experiment was laid out in RCBD with split plot arrangement having three replications. Hand weeded (weed free), and weedy check (control) plots were also mainta...

Misbahullah, Shad Khan Khalil, Asim Muhammad*, Afza Tabassum and Shah Fahad  

...e environmental friendly methods are needed to explore possibility of weed control and enhance crop yield. Sorghum extract and their application times on weed dynamics, phenology and leaf tracts of variety Atta Habib was sown at the Research Farm of Agronomy, The University of Agriculture Peshawar, Pakistan throughout 2015-16. Used of sorghum extract with three concentrations (1:3, 1:4, 1:5), weedicide (affinity) with three different levels (1/3, 1/ 2, 2/3 of ...

Babar Tasim1, Tariq Masood1*, Zafar Ali Shah1, Muhammad Arif2, Ata Ullah1, Ghazal Miraj3 and Muhammad Samiullah4 

...amples by using standard methods. Statistical analysis of the data revealed significant difference for proximate composition, pH, EC and macro and micro nutrients. Biochar obtained from Sesbania stem was found high in fixed carbon content (66.9%) and volatile matter (27%) but low in ash content (4.5%). AD biochar contained lowest amount of fixed carbon (49%) and volatile matter (17%) but highest ash content (30%) among different biochar samples under investiga...
Aqeel Mehboob, Syed Muhammad Zaka*, Muhammad Sarmadand Maryam Bajwa
... pesticides, alternative methods like botanical extract in the control of this pest consider greater importance nowadays. The antifeedant and oviposition deterrent index of Moringa oleifera, Murraya paniculata and Piper nigrum leaf extracts were evaluated against S. litura by choice and no choice method under laboratory controlled conditions. In M. oleifera extract, the maximum antifeedant index was recorded in 125.00 mg/ml (...

Naseer Ahmed Abbasi1, Mian Noor Hussain Asghar Ali1*, Bilawal Abbasi2, Shakeel Ahmed Soomro1, Najeeb Ahmed Khan Nangraj1, Jam Ghulam Mustafa Sahto1 and Sajjad Ahmed Morio1 

...parametric and fuzzy set methods in the land assessment for wheat production of Tando Allahyar district of Sindh Province. The present study able to provide grade performance suitability of lands for agricultural purposes; it could be more helpful for farmers of the research area. Fuzzy AHP methods could be beneficial for future studies for land suitability. The study finding have capabilities of different

Muhammad Aslam Rajput1,3, Imtiaz Ahmed Khan2, Rehana Naz Syed3 and Abdul Mubeen Lodhi3* 

...ix different inoculation methods, i.e., dipping, paste, wound+paste, soil infestation, spraying and injection methods for the establishment of smut disease. It appears that disease expression was significantly influenced by pathogen inoculation method. The infection caused by S. scitamineum and subsequent whip smut development has also been greatly varied with the inoculation method. Injection method followed by wound+paste ...
Khalid Khan1, Saima Liaqat2*, Somaiya Rasheed1 and Ihsanullah Kakar3
...r, orthodox and dogmatic methods, inadequate awareness and lack of animals’ hospitals lead to hamper livestock efficiency than the potential. Therefore, it is urged that government should make veterinary services available to the farmers at their door steps to improve their income, livelihood and standard of life. Moreover, this can be one of a feasible solution to the problems of poverty, malnourishment and starvation in backward and undeveloped areas l...
Muhammad Hidayat Rasool1,*, Muhammad Zaheer1, Muhammad Farooque Hassan2, Muhammad Shafique1 and Muhammad Usman Qamar1
...standard microbiological methods. Isolated GNRs were subjected to susceptibility testing against various antibiotics as per Clinical and Laboratory Standards Institute (CLSI) guidelines. Carbapenem-resistant isolates were subjected to detection of Metallo-beta-lactamase production by Double Disc Diffusion test (DDDT) and Modified Hodge’s test. In 152 specimens, different species identified in order of their frequency were; Klebsiella pneumoniae (5...
Shahid Yousaf1*, Salman Khurshid1, Saqib Arif1, Hafiza Mehwish Iqbal1, Qurrat ul Ain Akbar1, Najmus Sahar1 and Ghulam Rasul2
...using NIR and referenced methods shown to have average repeatability of 0.2%, 0.3%, 2.4% and 0.1%, 0.7%, 1.5% respectively. The information is useful for maintaining the quality of wheat intended for milling and ultimate products.
...
Doğukan Ölmez1 and Gonca Alak2,*
...re prepared by enzymatic methods at different time periods using different trout byproducts. In this study, two different groups (viscera and muscles) of trout processing wastes were hydrolyzed by enzymatic method based on specific time periods (0, 30, 60, 90, 120 and 180 min) for this purpose. Protein profiles, molecular size and distribution changes of the obtained hydrolysates were calculated using SDS-PAGE and lipid profiles were calculated using high perf...

Ayesha Khan*, Zubair Ahmad Khan, Urooba Pervaiz And Mehmood Iqbal 

...raints and the extension methods used by them. All agricultural officers and 40% of field assistants were interviewed making a sample of 81 extension agents; 10 agricultural officers and 71 field assistants. The study results indicate that 77% field assistants were educated up to Matric with two years diploma while 10% agricultural officers had M.Sc. (Hons) degree with the majority of extension agents having job experience of more than 20 years. The main sourc...
Tahira Moeen Khan* and Amat ul Mateen
...ensive chemical/physical methods. A cheaper, simple to perform method giving high yield without by products is employed here. The leaf extract act both as reducing and capping agent. The atomic absorption shows a linear curve between concentration and absorbance. The uv-vis spectrum revealed the shift of absorption towards longer wavelength with the increasing concentration of plant extract. XRD indicates CuO Nps particle size is in between 14-20 nm. CuO nanop...
Jason Shepard1 and Tyra Turner2
...e studies using big data methods to test whether the relation between future-oriented thought and well-being generalizes to non-WEIRD cultures. We found evidence that the relation between future-oriented thought and well-being was robust across cultures, including when analyzing a general trend across 64 countries (Study 1) and when examining specific cultural contrasts (Study 2 and Study 3). Taken together these three studies provide evidence that the relatio...

 Abdul Maalik1, Shahzad Ali1,*, Anam Iftikhar1, Muhammad Rizwan1, Haroon Ahmad2 and Iahtasham Khan1

...s analyzed by chi-square methods at 95% confidence interval. The overall prevalence of subclinical mastitis as a result of surf field mastitis test was 42.2%. The prevalence of disease was higher in cows (54.5%) than in buffalo (22.9%). Based on chi-square and P-value the prevalence of mastitis was significantly (P˂0.05) associated with animal type, urbanicity, age of animal and previous exposure of mastitis. There was no association of the prevalence of bovi...

Hafiz Umar Farid1, Muhammad Zubair2, Zahid Mahmood Khan1, Aamir Shakoor1*, Behzad Mustafa1, Aftab Ahmad Khan3, Muhamad Naveed Anjum4, Ijaz Ahmad5 and Muhammad Mubeen

... conventional irrigation methods. However, the most burning issue of these systems is their non-adoptability or low adoptability by the farming communities, despite their proven benefits. It was highly desirable to analyze and evaluate certain parameters which keep systems functional as well as ensure adoptability of these systems at farmer’s fields. A total of 30 sites were selected randomly in Punjab province of Pakistan where HEIS were installed. Twel...
Rıfat Ertekin1*, Mete Ozkurt1, Gokhan Kus2 and Selda Kabadere1
...uire that new, effective methods or chemicals should be developed. Tolfenamic acid (TA) is a nonsteroidal anti-inflammatory medicine stimulating apoptosis in cancer cells like pancreas, oesophagus and lungs. This study aims to determine the role of TA in cell viability and apoptosis in glioma cells (T-98G) and androgen-sensitive human prostate cancer cell line (LNCaP). In our study, 1, 5, 10, 25, 50, and 100 μM doses of TA were applied to T-98G and LNCaP ce...
Farhat Iqbal1, Abdul Waheed2,*, Zil-e-Huma3 and Asim Faraz2
...ast squares and Bayesian methods. Using the monthly body weight data of 2070 weight records of Thalli sheep from birth to 24 months of age for both sexes (male and female) and type of births(single and twin), the estimates of parameters and their credible intervals were obtained from posterior distributions. The overall goodness of fit of classical method was calculated; namely, Akaike information criterion (AIC), Bayesian information criterion (BIC), coeffici...
Emel Banu Buyukunal1,*, Rojan Ibrahim Albazaz1 and Mehmet Ali Bal2,3
...nventional and molecular methods for E. coli isolation in this study. Subsequently, antimicrobial susceptibility profiles of E. coli isolates (n=71) were determined against thirteen agents; amoxicillin-clavulanic acid, piperacillin-tazobactam, ceftazidime, cefotaxime, ceftriaxone, cefpodoxime, aztreonam, ciprofloxacin, amikacin, ertapenem, imipenem, meropenem, trimethoprim-sulfamethoxazole by disk diffusion method. Double-disk synergy test (DDST)...

Ayat Ullah* and Ayesha Khan 

...cal and cultural control methods in the rain-fed districts of Khyber Pakhtunkhwa, Pakistan. Using multistage random sampling technique data were collected from 395 respondents according to Yamane’s formula. The study uses the Kruskal–Wallis H test for checking differences among farmers knowledge with differences in their contact with extension service providers. The results revealed significant differences in farmers’ knowledge of chemical, b...
Pin Lyu1, Xiangxian Chen2* andQinlong Liu3*
...esponding rehabilitation methods. The injured muscular tissues and blood samples were collected pre-injury and post-injury on day 1, 2, 4, 6, 9, 12 and 15. The exercise and massage group showed the fastest recovery reflected by muscular histological structure and C-reactive protein levels, followed by the other three groups. C-reactive protein decrease in exercise and massage, massage only or exercise only groups was significantly different comparing to contro...

Waqar Akhtar1*, Munir Ahmad2, Nadeem Akmal1, Hassnain Shah1 and Asif Ali Mirani2 

...n traditional sun drying methods for processing of dates remained main source of low resource use efficiency of the system. Policy interventions needed to enhance the resource use efficiency in the production and processing of dates in study area through investment in research and development for processing and value addition technologies. 

...
Zhengfei Wang*, Dan Tang, Xuejia Shi, Huayun Guo, Xiuping Chen, Daizheng Zhang and Boping Tang*
...nt crabs by at least two methods. A series of putatively selected codons was localized in or close to the important functional regions (protein binding region and helical transmembrane region) in the mitochondrial protein structure.These results help explain why Bythograeidae crabs are capable of living in the hydrothermal vents and suggest that these crabs might have acquired an enhanced capacity for energy metabolism in an extreme hypoxic environment. These ...
Wafaa M. Hikal1,2,* and Hussein A.H. Said-Al Ahl3
...sistance to disinfection methods. The aim of this study was to isolate the pathogen from the dental units and hospital water network and treatment of Acanthamoeba with Trachyspermum ammi essential oil that have the ability to kill cysts. The amoebicidal activity of T. ammi oil was determined in vitro. Out of T. ammi oil showed that, higher concentrations (90 μg/mL after 72 and 96 hrs and 100 μg/mL after 24 and 48...
Ali Mujtab Shah1,2,3, Muhammad Naeem2, Muhammad Giasuddin Shah2, Muhammad Haaroon2, Quanhui Peng1 and Zhisheng Wang1,*
...arious colostrum feeding methods on growth performance, and immunity of Holstein Friesian (HF) calves. The applied feeding methods were stomach tube (ST), nipple feeder (NF) and natural suckling (NS). A total of 15 newly born HF calves were selected randomly from the available stock and divided into 3 groups, each of 5 calves. The median birth weight (kg) ± S.D of calves fed by ST, NP and NS were recorded as 28...
Qihang Liu1,2, Yueli Jiang1, Jin Miao1, Zhongjun Gong1, Tong Li1, Yun Duan1 and Yuqing Wu1*
...rol locusts, alternative methods such as photo-induced traps are elusive. In this report we investigated locusts visual sensitivity to different spectral lights after orange light stimulation. Results showed that after 10, 20, and 40 min of 1000 lx post-orange light stimulation, locusts visual response intensity was stimulated by green, violet, and blue lights. However, post-64600 lx orange light stimulation for 30, 20, and 10 min, the response intensity was t...
Qiuxia Lei1, 2, Haixia Han1, 2, Jinbo Gao1, 2, Yan Zhou1, 2, Wei Liu1, 2, Chunlei Wang3, Jie Liu1, 2, Dingguo Cao1, 2, Huimin Li1 , Baohua Huang1,* and Fuwei Li1, 2, *  
...To develop the effective methods for the innocuous treatment of the poultry manure, this study explored the efficiency of bacteria in decomposing the poultry manure through testing the variation of gas production and nitrogen(N), phosphorus (P), potassium(K)in the fermentation process. Poultry litter, sawdust and rice husk were mixed in equal quantities by volume. One was treated with a single strain of Bacillus; another was treated with a consortium of...

Muhammad Waqas, Shahid Ali*, Syed Attaullah Shah and Ghaffar Ali 

...d land and use different methods to make it arable for wheat production. Government also needs to make large reservoirs in unirrigated areas in order to store rainfall water for irrigation of unirrigated land for higher wheat production.

...
Shanshan Cai1, Tianxiang Gao2, Binlun Yan3, Aiyi Zhu1 and Xiumei Zhang1,2,*
...very, yet lack of proper methods hinders the precision and accuracy of such assessment. The swimming crab, Portunus trituberculatus is a commercially important species in Chinese fishery industry. However the natural resources of swimming crabs are declining and enhancement programs are being conducted for resources restoration for decades. In this study, 524 female broodstock from 10 hatcheries and 547 recaptured crabs from 6 investigations were used t...
Muhammad Mansoor1, Khalid Usman2*, Shahid Hameed Khan Khalil3, Abdul Mateen Khattak4, Muhammad Ehsan Elahi1, Muhammad Waqas Imam Malik2 and Amir Hamza2
...p;
...
Zhijun Zhong1, Rui Tu1, Xichun Wang2, Yi Geng1, Qicheng Xiao1, Yinan Tian1, Bin Wei1, Jiaming Dan1, Ya Wang1 and Guangneng Peng1,*
...n in pet dogs. Molecular methods, combining three types of PCR assays, identified two strains isolated from two pet dogs as Brucella canis. Histopathological changes revealed extensive inflammation and necrosis in the liver, lung, spleen, kidney, testicle, and lymph nodes, among which changes in the spleen were the most serious. Immunohistochemistry results demonstrated the detection of B. canis antigens in the lesions of all examined tissues. St...
Qi Ren, Shufeng Sun, Chen Niu, Yuhong Li, Yingzhi Chong, Biao Li, Guoying Zheng and Fumin Feng*

 

...ation-specific PCR (MSP) methods. The increases in the methylation rate of CpG islands in the CYP1A1 promoter region and expression of DNA methyltransferases (DNMTs). And then the CYP1A1 mRNA and protein expression decreased with cell damage, thus suggesting that elevated methylation of the promoter region of the CYP1A1 gene may affect its expression and could be associated with cell damage. After treatment of the cells with AZA, increases in methylation rate ...

Illahi Bux Bhatti1, 2*, Imran Khatri2, Maqsood Anwar Rustamani2 and Riffat Sultana

...ion of different control methods i.e. cultural, biological, yellow sticky traps and chemical against sugarcane whitefly during February 2016 at PARC-NSTHRI, Thatta, experimental field. The result revealed that lowest number of whitefly (0.38 nymphs + puparia per cm2 per leaf) with highest % mortality (79.89) was recorded in T11 (cultural control + biological control + chemical control), followed by T10 (Cultural control + Biological control + Yellow sticky tra...

Muhammad Khuram Razzaq1,2*, Saeed Rauf1, Mohsin Khurshid3, Shahid Iqbal1,4, Javaid Akhter Bhat2, Ayaz Farzand5, Adeel Riaz1,6, Guangnan Xing2 and Junyi Gai

 

...viability, selection and methods for the improved pollen viability and the management of heat stress.  

...

Yousaf Noor*, Zahir Shah and Muhammad Tariq 

...ct of Zn and B and their methods of application on the yield and yield attributes of citrus (Citrus Sinensis) orchards established on calcareous soils at three farmers fields in Dubandai and Palai area of District Malakand of Khyber Pakhtunkhwa, Pakistan during 2009-2010. A randomized complete block experiment with split plot arrangement was used. Methods of application (Soil and Foliar) were assigned to main plots while mic...

Shwe Yee Win1, Lat Lat Htun1, Myint Myint Hmoon1, Hla Myet Chel1, Yu Nandi Thaw1, Nyein Chan Soe1, Htet Lin Oo3 and Saw Bawm2* 

...tation and sedimentation methods were carried out to detect the gastrointestinal parasites. The overall occurrence of gastrointestinal parasites in village chickens was 60.5% (121/200). Six species of gastrointestinal parasites were observed as Capillaria spp. (30.0%, 60/200), Ascaridia galli (24%, 48/200), Eimeria spp. (20.5%, 41/200), Raillietina spp. (17%, 34/200), Strongyloides spp. (2.5%, 5/200) and Subulura brumpti (2.0%, 4/200). Mixed infection rate was...
Abu ul Hassan Faiz1,*, Fakhar-i-Abbas2, Mehboob ul Hassan3 and Lariab Zahra Faiz1
...nd for birds point count methods were used to determine abundance of each species. The study, documented 10 species of mammalian pests, 13 species of pest birds of maize crop and their population density differed significantly (P≤ 0.05) at study sites. The habitat analysis of study sites and correlation of species with habitat were done through Principal Component Analysis (PCA) by using software (XLSTAT-Ecology version). The habitat was positively correlat...

Turab Ali Kaurajo1*, Huma Rizwana1, Gulbahar Khaskheli2, Muhammad Haroon Baloch1, Muhammad Naeem Rajput1, Asad Ali Khaskheli3 and Mohsin Solangi

...by using old traditional methods. The modern farming does not exit and extension services are rarely available to motivate and educate the camel herders. Higher load carrying capacity, hair production and body weight was observed in Kharai breed, however; milk production and lactation length was better in Dhatti breed. 

...

Manzoor1*, Ahmad Khan1, Amir Sohail2, Shahzad Ali1, Fawad Ali Shah3, Junaid Iqbal3, Muhammad Owais Khan4 and Sultan Nawaz4 

...le of different planting methods (Ridge, Flat and Broadcast) in soil moisture conservation for maize crop. The research was carried out using RCBD with split plot arrangement having 4 replications at Agronomy Research Farm, The University of Agriculture Peshawar, during May 2017. Deficit Irrigations were allotted to main plots, while planting methods were allotted to sub plots. Deficit irrigations had significant (P≤0.05)...
Chiping Kong1,2, Yongheng Wu1,2, Shangling Lou1, Benping Chen3, Simon D. Dowell4 and Yiqiang Fu1*
...an, China. We used three methods to compare the acoustic difference among and within individuals. The results demonstrated that male Emei Shan Liocichlas had individually distinct songs and can be used to identify individuals. We also found that 4 to 5 songs were relatively certain of an individual identity. Our findings indicated that individual distinctiveness by vocalisation was reliable in the Emei Shan Liocichla. Monitoring song therefore provides a poten...

Awais Ahmed Khan1*, Zafar Iqbal1 and Muhammad Atiq2 

...d to develop alternative methods to synthetic fungicides, currently used to control green mold of citrus caused by the Penicillium digitatum. Antifungal activities of organic elicitors were evaluated and all the elicitors expressed significant results. Salicylic acid was the most effective to control the disease and showed minimum mycelial growth diameter (35mm) followed by benzoic acid (44mm), jasmonic acid (45.5), and ascorbic acid (50mm) as compared to cont...

Muhammad Qaisar Nawaz*, Khalil Ahmed, Ghulam Qadir, Muhammad Rizwan, Muhammad Faisal Nawaz and Muhammad Sarfraz 

... the cost-benefit sowing methods for turnip production under moderately salt-affected soils. The experimental design was split-plot with sowing methods (bed and ridge) in the main plot and nitrogen doses (40, 60, 80 and 100 kg ha-1) as sub plot. Treatments were replicated three times with sub plot size of 4 m × 6 m. Measurements included were: number of plant/m-2, number of leaves/plants, root length, bulb diameter, fo...
Nurlybay Kazhgaliyev1*, Talgat Kulmagambetov2, Dulat Ibrayev1, Saule Bostanova1 and Zhanat Titanov1
...tigate common zootechnic methods, daughters of imported Aberdeen Angus and Hereford cows were grouped at the age of 18-19 months with a live weight of no less than 350 kg. Groups were constituted using the analogue-pairs method and consisted of 30 cattle/group. Observations during the present study showed that the average live weight of Hereford heifers of the second generation at the age 18-22 months was 378.5 and 375.2 kg which was 8.7 and 20.6 kg (2.2-5.4%)...
Monica Paula Marin1, Elena Narcisa Pogurschi1*, Iuliana Marin2 and Carmen Georgeta Nicolae1
... natural, less expensive methods for improving the milk quality are the most studied by farmers and researches. Clinoptilolite, natural zeolite added to dairy cows diets, has been proven to be an efficient and economic solution. Starting from the fact that natural zeolites are recognized as having beneficial effects on the animal organism, being detoxifiers, immunomodulators, regulators of the internal pH, we studied the effect of using volcanic stuff as a foo...

Kalpana Polisetty1* and Kiran Kumar Paidipati2 

...prominent non-parametric methods i.e. Pettitt’s, Standard normal homogeneity (SNH) and Buishand’s range tests. The core purpose of these three techniques is to capture the mutation point in time series data connected to wheat production during the period 1980-2016. Consequently, Sen’s slope estimator has been used to measure the magnitude of the trend for wheat production and their significances examined through Mann Kendall (M-K) test. The a...
Ramazan İlgün1*, Nilgün Kuru2, Ferhan Bölükbaş3 and Fatih Mehmet Gür4
...ning electron microscopy methods. Six chicks (6-7 weeks), six layer hens (9-13 weeks) and six studs guinea fowl were used to study and determine the histological features of the tongue of the guinea fowl. Histological structures were examined using a light microscope the tissues and papillae of the tongue were examined with a the scanning electron microscope, and photos of the general histologic structures were taken. The tongue was triangular shaped, and cons...
Nihat Yeşilayer*
... visual and colorimetric methods, of three fish species. Linear relationships were observed between CIELAB colour measurements (lightness-L*, redness-a*, yellowness-b*, hue-H°ab and chroma-Cab), DSM SalmoFan card scores and TCCs. The redness (a*) was mostly correlated with TCC of the fish fillets. The FRT group had the highest TCC (9,75 mg kg-1) compared to the other two species. However, DSM SalmoFanTM card score was hi...

Muhammad Ilyas1, Sardar Ali Khan1, Shahid Iqbal Awan1, Shafiq-ur-Rehman1, Waqar Ahmed1, Muhammad Riaz Khan2, Raja Mohib Muazzam Naz2, Muhammad Mohib Ullah Khan3 and Sumaira Hafeez1* 

...e quantitative biometric methods based on measurement of phenotypic performances of certain quantitative traits on as many as possible plant individuals in basic experimental generations. Initially, 108 inbred lines of maize were planted in two separate trails under water deficit and normal irrigation conditions in the experimental field of Department of Plant Breeding and Molecular Genetics. Selection of parents for further study was done with regards to grai...
Muhammad Irfan Ullah1*, Muhammad Arshad1, Sajjad Ali2, Asad Abdullah1, Samina Khalid3, Hafiz Muhammad Aatif4, Syed Muhammad Ali Zahid1, Muhammad Afzal1 and Jaima Molina-Ochoa5
...s impartment. The recent methods used to quantify the foliar damage are relatively expensive and are mainly dependent on complex and state of art devices and instruments. Instead, smartphone application may be a good source to measure the foliar damage. In this study, foliar damage of Spodoptera litura Fab. (Lepidoptera: Noctuidae) on sunflower and soybean crops was measured using BioLeaf android application. The infected leaves due to citrus canker were also ...
Larysa Kladnytska1, Anatoliy Mazurkevych1, Natalia Bezdieniezhnykh2Oleg Melnyc1, Sergiy Velychko3, Mykola Malyuk1, Vasyl Danilov1Yuriy Kharkevych1 and Magdalena Gryzinska4*
Sohail Ahmed1,*, Muhammad Waseem Saleem1, Abid Ali1, Muhammad Shahid Nisar2, Rashad Rasool Khan1 and Abdul Rashid3
...ation with other control methods proved to be the most effective treatment. Releases of bio-agents viz., C. septempunctata and C. carnea resulted in 4.72 and 4.51 thrips leaf-1, respectively, and also did not differ significantly with each other. Application of bio-agents in combination was less effective with 4.37 thrips leaf-1 and differed significantly from all other treatments. Application of NKSE resulted in 4.00...

Shah Nawaz Khuhro1*, Irshad Ali Junejo1, Muhammad Haroon Hullio1, Sultan Ahmed Maitlo2, Javeed Shabir Daar3 and Shahjahan Rajput

...lor traps may be used in methods of insect population monitoring. The other colors were less attractant so this study found that using yellow-colored sticky-traps as an alternative for Brinjal crop for the monitoring and management of whitefly. On the basis of results it is suggested that sticky traps are the most excellent integrated pest management approach to prevent problems from increase of whitefly population in the brinjal crop. Further is suggested tha...

Shah Nawaz Khuhro1*, Irshad Ali Junejo1, Muhammad Haroon Hullio1, Mohammad Farooque Hassan2, Sultan Ahmed Maitlo3 and Mukhtiar Ahmed Shaikh

...sing more on alternative methods for sustainable crop production and by reducing the use of pesticides and trainings on pest counting helps in sustainable pesticide usage in Pakistan. Randomly 66 farmers were selected to conduct the interview for background information about the growers’ status on pesticide application. The results show that the majority of the growers did not get any kind of trainings on sustainable use of pesticides. Results indicated ...

Ghani Akbar 

...e to traditional farming methods. Therefore, this paper is aimed to investigate the key agricultural issues and to identify strategies for utilizing the full potential of agriculture for improving livelihood in this region. This study indicated that climate change induced hazards of land degradation and water scarcity due to frequent floods and droughts, decline in traditional springfed Kuhl (gravity channel) irrigation system, low productivity of crops and ho...

Wajiha Anum1*, Liaquat Ali1, Umair Riaz2*, Abid Ali1, Nadia Manzoor3, Laal Hussain Akhter4, Asad Ur Rahman5, Naeem Maan6 and Ijaz Ahmad

...t of various P placement methods and fertilizer rates on wheat. The fertilizer treatments were F1=150-00-60 NPK kilogram/hectare, F2=150-30-60 NPK kilogram/hectare, F3=150-60-60 NPK kilogram/hectare, F4=150-90-60 NPK kilogram/hectare, F5=150-120-60 NPK kilogram/hectare. While the placement methods were M1=Line sowing + Band application of phosphorus, M2=Line sowing +Broadcast application of Phosphorus at sowing, M3=Line sowi...

Ghani Akbar*, Muhammad Asif, Zafar Islam and Shahid Hameed 

...velled border irrigation methods are the norms on majority of Pakistani farms, which is inherently inefficient. Sprinkler and drip irrigation are costly and require more technical knowledge, thus negligibly adopted and are generally considered as late time solutions. Although furrow bed irrigation, a relatively more efficient irrigation method, has been adopted, especially for row crops but decision support guidelines for their appropriate installation and man...
Hina Nazli, Abida Butt*
...cal and chemical control methods are required to successfully manage insect pests. Along with insect pests, many predators of these pests are also present in crops and affected by these management activities. Spiders are the most abundant predators of insect pests in the agroecosystem. The present study was designed to assess and compare acute and residual toxicity of two insecticides i.e Spiromesifen and Thiamethoxam on the lynx spider Oxyopes javan...
Fehmina Ashraf1*, Muhammad Irfan2, Hafiz Abdullah Shakir1, Shaukat Ali3, Muhammad Khan1
...been purified by various methods. It involves in di-oxygen to water reduction and 1e- oxidation of phenolic and its allied parts. Laccases are mostly used in different industries like food, paper and pulp, pharmaceutical and textile etc. Both laccases and mediators have been used in different process like delignification of pulp. Laccases from bacterial species are used in dye decolouration and biobleaching processes. This review article describes the whole ov...

Md. Al-Amin Tan1, Mst. Antora Akter1, Md. Sabuj Rahman1, Marzia Rahman2 and Md. Mahmudul Alam1* 

...f skin disinfectants and methods of skin preparation is still aberrant in veterinary practice. The study was, thus, conducted to standardize pre-surgical skin preparation with various formulations of Chlorhexidine gluconate, Chlorxylenol, and Povidone-iodine (PI) in goat. Seven surgical fields were prepared for evaluating seven formulations of these antiseptics. The bacterial swabs collected at different stages of skin preparations were transferred in nutrient...

Joseph Anejo-Okopi1*, Ocheme Julius Okojokwu1 and Onyemocho Audu2 

...nd inexpensive molecular methods for accurate diagnosis. The sub-Saharan Africa HCV genotypes distribution are diverse, but genotype I is the most common. This review highlights the need for more robust surveillance studies with introduction of opt-in testing at all clinics for better epidemiology the HCV disease burden more accurately.  

...

Rana Shahzad Noor1,2*, Fiaz Hussain2, Muhammad Umair2, Waqas Aslam2, Muhammad Ameen4, Muhammad Adnan Islam3 and Muhammad Sheraz2 

 

... under different tillage methods was 2097.8 kg ha-1, while the lowest yield was observed as 1649.9 kg ha-1 respectively. The fuel consumption of main crop was measured between 33.33 l ha-1 and 63.48 l ha-1, while second crop fuel consumption was ranged as 34.92 l ha-1 to 62.48 l ha-1. The highest and lowest fuel consumptions levels of different soil tillage for main and second crop peanuts were 62.61 and 34.08 l ha-1 and 33.09 and 61.82 l ha-1, respectively. T...

Nasir Ahmad1*, Jehad Ali2, Khalil Khan3, Muhammad Naeem4, Usman Ali5 

ROBUST MULTIMODAL FACE RECOGNITION WITH PRE-PROCESSED KINECT RGB-D IMAGES
...r applicability of these methods is inadequate in practical implementations
due to high cost of 3D sensors, therefore we are using the low cost sensor Kinect acquired images. We do
face recognition from RGB images, depth images and then we combine both RGB and depth maps i.e. concatenate
different Modalities to improve the accuracy of recognition. Depth maps have holes and noise induced from camera
sensors, therefore we process them...

Misbah Ullah1, Rehman Akhtar1, Sikandar Bilal Khattak1, Khawar Naeem1 and Khizar Azam2 

FACTORS IDENTIFICATION FOR COAL BRIQUETTES MANUFACTURING USING STATISTICAL EXPERIMENTAL DESIGN APPROACH
...red through conventional methods in developing countries including Pakistan.
Shortages of energy resources across the globe have pushed companies to manufacture those products with least
possible costs by using significant factors. Indeed, the role of statistical design of experiment in general and factorial
design in particular is vital in identifying these significant factors in comparison to the conventional techniques. This
pape...

Tehmina Amjad1, Ishfaq Ahmad1, Muhammad Akram Shaikh2, Nabila Naz1 

VARIATION IN CITATION BASED FRACTIONAL COUNTING OF AUTHORSHIP
...

...

Nazish Huma Khan1, Mohammad Nafees1, Adila Bashir1, Farooq Ahmad1 

STUDY OF POLLUTION LAOD IN PAPER MILL EFFLUENTS AND ITS RECYCLING BY SUNDRY PLANTS AT HAYATABAD INDUSTRIAL ESTATE, PESHAWAR
...D5) by adopting standard methods for the
examination of water and waste water. These samples were also investigated for micronutrients/heavy metals (Cr, Pd,
Cd, Ni, Fe, Zn and Cu), using Atomic Absorption Spectrophotometer. The obtained results were compared with Pak-
NEQS for industrial effluents. Results showed high limits of pH(8-8.2), EC(738-764uS/cm), Alkalinity(463-581mg/l),
Turbidity(166-180NTU), TSS(967-1063.5mg/L), TDS(1673...

Asif Nawaz1*, Muhammad Zafarullah Khan1, Rehmat Ullah1, Arshad Farooq2 and Abdul Hassan2

Assessment of Weed Management Competency of Field Assistants in Khyber Pakhtunkhwa, Pakistan
...t indirect weeds control methods. Moreover, it was found that Field Assistants required highest training needs in familiarity with biological weed control followed by critical threshold level of weeds and critical period of weed competition. Findings of Rank Based Quotient revealed that lack of promotion, training opportunities and incentives or motivation were the top most constraints that respondents faced in building the required competencies. Therefore, it...

M. Rizwan Gul*

COMPARISON OF THE OF MECHANICAL AND WEAR BEHAVIOURS OF DIFFERNT TYPES OF POLYETHYLENES AND THE EFFECT OF RADIATION CROSS-LINKING ON THESE BEHAVIOURS
...e of the most convenient methods. This study explores the difference in mechanical and wear behavior of different polyethylenes, and compares the effect of radiation cross-linking and radiation dose on these properties. Three different types of polyethylenes: low density (LDPE), high density (HDPE), and ultra-high molecular weight (UHMWPE) were studied. Cross-linking was carried out by high energy electron beam at room temperature, with radiation dose ranging ...

Muhammad Imran Ahmad*, Nan Zhang**, Megan Jobson**, Muhammad Younas*, Kamran Ghani***

DELUMPING PROCEDURE FOR PREDICTION OF DISTRIBUTION OF PRODUCTS IN DISTILLATION USING A SHORT-CUT MODEL
...ng short-cut calculation methods. The proposed methodology helps retain molecular information of composition of petroleum fractions and may help extend the premise of molecular components-based modeling to separation processes such as distillation.
...

Muhammad Naeem*, Sohail Asghar**

A REVIEW OF FEATURE SELECTION TECHNIQUES IN STRUCTURE LEARNING
... corresponding searching methods under six well known scoring functions in K2 which is a notable structure learning technique in Bayesian belief network. We have come up with some useful findings for overall computationally efficient approach among eleven evaluators. This analysis is useful in inducing better structure from the given dataset in imparting improved performance metric for Bayesian belief network.
...

Shahid Ali1, Habib Akbar2, Shamsher Ali3*, Adnan Nasim1, Muhammad Ismail1, Nur Ul Haq1 and Muhammad Usman4

Effect of Planting Sources, Cane Portions and Setts Placement Methods on Sugarcane Yield Attributing Traits
...ions and setts placement methods on sugarcane yield attributing traits were examined at Sugar Crops Research Institute (SCRI), Mardan, Khyber Pakhtunkhwa Pakistan. The experiment was conducted in RCB Design with split plot arrangements, in 3 replications for two years (2012-14). Different planting material i.e., trenched (sugarcane buried in soil to avoid frost damage) and fresh cane (directly obtained after harvesting of standing crop) stalk segments i.e. upp...

 Gulzar Ahmad1, M. Inayatullah Babar2

AN EFFICIENT PATCH ANTENNA WITH IMPROVED BANDWIDTH FOR FUTURE APPLICATIONS
...s 1.64mm. Different methods were engaged to expand its bandwidth from 9.07GHz to 13.13GHz. The reflection coefficient of -45dB at the central frequency, a gain of 7dBi and total efficiency of 0.99 have been demonstrated by this proposed design of the patch antenna. This novel structured wide band and high efficient antenna can be engaged in various types of wireless applications in near future.

...

 Saman Shahid1, Shahid Ali1, Muhammad Abdullah Ahmad1, Sarfraz Munir2

RE-DESIGNING AND OPERATIONAL ANALYSES OF THE SEDIMENT TRANSPORT CONTROL IN IRRIGATION CANAL NETWORK
...ngelund Hansen predictor methods. The data related to BRBD canal, including its cross-sectional properties, hydraulics and sediment parameters were acquired from the Irrigation Department, Lahore. The paper also described the problem which exists in design and operation of the canal by comparing it with original field data. The BRBD canal achieved a regime condition in 1.5 years. The results of sediment deposition produced by Brownlie predictor were more accur...

 Nashitah Alwaz1, Safdar Raza1*, Sadaqat Ali1

A COMMUNICATION-FREE SIGNAL GENERATING TECHNIQUE FOR REACTIVE POWER CORRECTION IN ISLANDED MICROGRID
...mands. The droop control methods are normally used for appropriate power sharing. However, these control techniques suffer badly due to the feeder impedances and uncontrollable power coupling issues. Many techniques have been proposed to share power accurately, most of them rely on communication
link. In this work a localized/communication free signal generating mechanism is proposed. The mechanism injects&nbs...

Altaf Hussain Rajpar1, Abdul Hameed Memon1, Mohammad Akram Shaikh2, Weimin Zhang3

HAND-EYE COORDINATION FOR OBJECT GRASPING TASK OF HUMANOID ROBOT (BHR-02)
...Least Square Error (LSE) methods. For hand-eye coordination a hand-eye model was developed to adjust the position and orientation error for object grasping task. In the hand-eye model world coordinate system was set on right shoulder joint. The position and orientation of the hand was obtained through coordinate transformation from left eye coordinate system to end-effector coordinate system. The results presented in this paper justify the ...

 Nisar Mohammad1, Mohammad Mansoor Khan1

RECOVERY OF TALC FROM TALC- CARBONATE SCHIST OF SWAT EMERALD MINES, NWFP, PAKISTAN
...ration and leaching methods were conducted to see the amenability of talc to enrichment by these techniques. Analysis of seven talcum powder products available in the local market including pharmaceutical grade talc was carried out for comparison with Swat raw- talc under study and to know the level of impurities in it. The results revealed that Swat emerald mines talc rock contains calcite, magnesite, dolomite, muscovite and quartz as impu...

 Faizullah Mahar1, Syed Saad Azhar Ali1, Abid Karim1

DESIGN OF FIXED ORDER ROBUST CONTROLLER USING H∞ -NORM AND EVOLUTIONARY TECHNIQUES: COMPARISONS AND PERFORMANCE ANALYSIS
...oreover, in these design methods the order of controller cannot be fixed a priori to control law design. In industrial applications it is hard to execute these controllers practically. To overcome this problem, this paper proposes the use of evolutionary techniques (i.e. genetic algorithm and immune algorithm) for the designing of a low, fixed order robust controller. To check the effectiveness of the proposed approach, resulting controller...

Rafi Javed Qureshi* and Iftikhar Hussain**

INTERNATIONALIZING MANUFACTURING SMEs IN A DEVELOPING REGION: AN EXHAUSTIVE CDA-BASED EXPLORATORY APPROACH
...p;in question. The three methods relevant to categorical data analysis (CDA) in this paper highlights the enabling role of the exploratory factors in understanding the relationship with SME’s exporting. Categorical regression overcomes the limitations of both contingency table method and binary logistic regression in exploring this relationship, and provides substantive space to experiment and investigate the contributory role of expo...
Ayesha Noreen1, Amina Elahi1, Dilara Abbas Bukhari2 and Abdul Rehman1*

 

...etric and molybdene blue methods) at pH 7 and 37°C in the presence of Zn2+ as cofactor. The protein profile of B. cereus 3.1S, showed two bands of approximately 14 and 70 kDa, which had their possible role in arsenite oxidation. This was confirmed by transforming E. coli DH5α with plasmid DNA of B. cereus 3.1S. This arsenite resistant bacterial strain oxidized 76 and 86.5% As3+ from the original industrial w...
Shikun Ge1, 2, Yanli Yang3, Brindha Chelliappan4, 5, Antonysamy Michael5, Fagang Zhong1 and Xiaoying Zhang1, 2, 3, 4*
... However, the extraction methods and storage stability of IgY antibodies from egg yolk for feed supplements remain major problems in mass production. Based on Based on IgY antibody titer, IgY thermostability, IgY acid and alkali resistance, resistance to trypsin and pepsin, four different IgY purification methods (PEG extraction, water dilution method, the effect of liquid egg yolk and egg yolk powder preparations) on the ac...
Huma Abbas1*, Nazir Javed1,Muhammad Kamran2, Sajid Aleem Khan1Hira Abbas3, Ehetisham-ul-Haq2, Abdul Jabbar1, Mehwish Naz1 and Ihsan Ullah4
...of different application methods; soil drench and root dip was also tested. Results revealed that all the chemicals have more or less systemic effect against M. incognita. Cartap was found to be more effective in reduction of egg masses followed by Virtako in both treated and untreated root portion. Number of egg masses was reduced significantly in Cure and Azadirachtin in treated (45.7, 58.2) as well untreated root portion (93.7, 80.7) respectively. In...
Ishrat Perveen1, Yasar Saleem2 and Javed Iqbal Qazi3* 
...high temperature cooking methods and higher quantities of HCAs in RTE Chicken kabab commercially available from Pakistani markets.
...

Ummad ud Din Umar1, Syed Atif Hasan Naqvi1*, Ishfaq Ahmed1, Ateeq ur Rehman1, Muhammad Asif Zulfiqar2, Sibghat Ullah3, Shah Pasand4, Zehri Khan3 and Abdur Rehman5

Prevalence and Serological Detection of Spiroplasma citri, a Cause of Citrus Stubborn Disease in Southern Punjab, Pakistan
...ological based detection methods by conducting a comprehensive survey in various districts of Southern Punjab. The results of the survey and serology of CSD showed that this destructive disease which causes symptoms mostly similar to citrus greening is present in all areas of South Punjab. Layyah, and Multan showed maximum incidence of CSD with “11.66” and “5.66%” respectively while least incidence was observed in District Bhakkar (03%)...

Md. Aminul Islam1*, A.N.M. Aminoor Rahman2, Mohammad Shah Alam3 and Md. Taimur Islam4

Gastrointestinal Nematodiasis in Quail in Bangladesh
...analyzed by direct smear methods and nematodes identified by the presence of characteristic eggs in the feces. Five types of nematode eggs found on different farms. The overall prevalence of gastrointestinal nematodiasis were (17.5%), which could consider relatively higher. The highest rate of infection found for Heterakis gallinarum (7%) and the lowest rate of infection found for Trichuris spp. (1.5%). A significant difference in nematodiasis in different far...

Christian Marco Hadi Nugroho1 and Jeanne Adiwinata Pawitan2,3,4*

Production of Antibody for Direct Fluorescence Antibody Assay against Avian Influenza H9N2
...ies (OIE) suggested some methods for the detection of H9N2 infection including the immunofluorescence method that is commonly known as the direct fluorescent antibody (DFA) assay. This method is a combination of immunology and bio-imaging. This DFA assay is rapid and sensitive, but specificity is influenced by antibody production methods. Therefore, the aim of this review was to describe various antibody production
Muhammad Ilyas1,2*, Imran Khan2*, Muhammad Umer Chattha2, Muhammad Umair Hassan2, Muhammad Zain3, Wajid Farhad4, Sana Ullah1, Ateeq Shah5, Safeer Ahmed6, Bismillah Khan1 and Muhammad Adeel1
Evaluating the Effect of Zinc Application Methods on Growth and Yield of Wheat Cultivars
...f various Zn application methods on the growth and yield of wheat cultivars during the growing season 2014-2015. The study was comprised of three cultivars of wheat; Galaxy-2014, Punjab-2011 and Millat-2011 and Zn application methods; seed priming @ 0.4% Zn solution, soil application @ 40 kg ZnSO4. H2O ha-1 foliar spray @ 0.5% Zn solution at vegetative stage and combination of all three methods
Taqiur Rahman1, Tabassum Yaseen1*, Ali Mujtaba Shah2, Samiullah3, Ghulam Jelani3, Gul Nawaz1,Qudratullah Kalwar2
...s; the methodologies and methods used to treat diseases, maintaining animal health and its importance to ethnobotany and Pharmacogonosy.The objective of the present study is to identify the leading medicinal plants used by local communities for the treatment of animals and document the threatened herbal knowledge from the elder formers (R1, R2 and R4) this study labels the ethno veterinary practices in different villages of District Shangla, where the document...
Muhammad Zeeshan Majeed1*, Muhammad Afzal1,Muhammad Asam Riaz1,Kanwer Shahzad Ahmed1, Muhammad Luqman2, Mehar Zubair Shehzad1, Muhammad Bilal Tayyab1, Mujahid Tanvir1 and Saadia Wahid1
...ilter paper-dip bioassay methods, respectively. Results revealed that the extracts of Mentha longifolia, Sonchus asper and Nerium indicum were the most toxic to D. citri exhibiting 90% mortality. The extracts of Dodonaea viscosa and Olea ferruginea caused highest mortality of S. litura (i.e. 70 and 58%, respectively). Maximum mortality of C. quinquefasciatus larvae was observed by Maerua ...

Agustine Christela Melviana1, Rizkita Rachmi Esyanti1*, Roy Hendroko Setyobudi2, Maizirwan Mel3, Praptiningsih Gamawati Adinurani4 and Juris Burlakovs5

Gene Expression Related to Steviol Glycoside Synthesis Produced in Stevia rebaudiana (Bert.) Shoot Culture Induced with High Far-Red LED Light in TIS RITA® Bioreactor System
...via plants using in vivo methods, an alternative technique of propagation using in vitro methods is needed. The micropropagation method with the RITA® (Recipient for Automated Temporary Immersion System) is used in the production of a large amount of stevia biomass in approximately a short period. The forming of flowers is one of the limiting factors interfering with the metabolite production, as the content of steviol g...

Muhammad Kashif Khan1*, Muhammad Wasim Jan Khan2, Kaleem Ullah Jan Khan3, Muhammad Ishaq Khan4, Muhammad Faheem Jan Khan5 and Abdul Salam Lodhi6

 

Impacts of Construction Risk Attributes on Organizational Performance of Registered Construction Companies in Khyber Pakhtunkhwa, Pakistan
...tigation through various methods and techniques. The purpose of this study is to address the need of risk management practices in the construction industry of Khyber Pakhtunkhwa (KPK), Pakistan. The research work carried out with the involvement of 150 Pakistan Engineering Council (PEC) registered construction companies not only emphasizes on the crucial risk factors associated with construction projects but also insights into the impacts of the risk factors o...

Mehreen Ijaz

Effect of Weave Type on Tensile Strength of Cotton Fabrics
Rehman Akhtar1, Khizar Azam2, Abdur Rehman Babar1, Qazi Salman Khalid1, Rashid Nawaz1 and Imran Ahmad1 
 
...ost frequently used MCDM methods in literature, the Analytical Hierarchy Process (AHP) is integrated with fuzzy logic, called as F-AHP, is used in this research to identify and evaluate solar farm location in division of Khyber Pakhtunkhwa (KPK). Thana, Chakdara, Harichand, Palai, Pir Khel and Shergarh city are evaluation by Fuzzy AHP on the basis of above-mentioned factors. Shergarh city is selected for most suitable location for the installation of solar pan...

Shawaiz Iqbal1*, Nadeem Iqbal2, Usama Bin Khalid1, Muhammad Usman Saleem1, Adila Iram1, Muhammad Rizwan1, Muhammad Sabar1 and Tahir Hussain Awan1

Growth, Yield and Economic Analysis of Dry-Seeded Basmati Rice
...e the different planting methods. Three dry-seeded rice (DSR) planting methods viz. (i) DSR-broadcast, (ii) DSR-drill, (iii) DSR-ridges were compared with the conventional transplanting (TR) method in lines having row to row and plant to plant distance of 22.5cm distance, and farmer transplanting method. Experimental design was randomized complete block, replicated 3 times. Data were recorded on height (cm), tillers m-2, fil...
Wentao Wang1,2, Xu Lin3, Jianshu Zhuo3, Dongjie Zhang2, Xiuqin Yang3* and Di Liu1, 2*
...edicted by bioinformatic methods. The results increase our knowledge of E2F3b mRNA diversity and provide basis for in-depth functional research.
...
Minghao Gong1*, Shiliang Pang2, Zhongyan Gao2, Wanyu Wen1, Ling Zhang3, Gang Liu1, Huixin Li1, Fawen Qian4 and Wenfeng Wang2
...lso, organic agriculture methods and crop species that consume less water than rice should be adopted to mitigate the adverse climate changes that accompany global warming and will contribute to RCC habitat degradation.
...
Alam Zeb* and Amanullah Jan
...onse of sesame to sowing methods, nitrogen and sulphur levels” were carried out in Agronomy Research Farms, The University of Agriculture, Peshawar. During two consecutive Kharif growing seasons of 2013 and 2014, respectively. Three sowing methods (flat, ridges and raised bed) were kept as main plot factor, whereas four nitrogen levels (00, 60, 120, and 180 kg ha-1) and four treatments of sulphur (...

Hillary M.O. Otieno

A Review of White Mango Scale (Aulacaspis tubercularis Newstead; Hemiptera: Diaspididae) in Sub-Saharan Africa: Distribution, Impact and Management Strategies
...tive traditional control methods. Farmers capable of using pesticides apply very toxic products that are not only harmful to the applicators but also to the beneficial organisms. Agronomic management strategies like right varieties, spacing, better fertilization and shed management are also applied inappropriately. This review is therefore essential in providing the basis of identification of A. tubercularis. It also provides management strategies that could b...
Tariq Mahmood Khalil1,2*, Muhammad Ajmal3, Iftikhar Zeb1,4 and Muhammad Azeem Khan1,5
 
 
...the existing traditional methods for measuring the soil salinity. It is also expected that this technique will also help in calibrating the existing salinity measuring devices and may minimize the influence of bias or prejudice induced due to instrumental errors.
...

 Shuanping Zhao, Lei Xu, Hai Jin and Yutang Jia*

...ng and (forced) PCR-RFLP methods, three polymorphisms (g.1500G>A, g.1694T>A and g.2213C>G) were identified and genotyped in our population (n=298). Genetic diversity analysis showed that g.1500G>A and g.1694T>A belonged to an intermediate level of genetic diversity (0.25<PIC<0.5), and SNP g.2213C>G belonged to a low polymorphism level (PIC<0.25). LD (Linkage disequilibrium) analysis showed that SNP g.1694T>A and g.2213C>G had a...
Huan Yang1, Tingting Li1, Huimin Zhao1,2, Xiaoyuan Zhang1, Huiyan Xu1, Yangqing Lu1, Xingwei Liang1, Shengsheng Lu1, Xiaogan Yang1*and Kehuan Lu1*
...lo SSCs in vitro, methods for their isolation, enrichment, culture and preliminary identification were established. Testes from 3- to 6-month-old buffalos were digested with two-step enzymatic treatment to obtain isolated single cells and then enriched with a differential plating method. Isolated SSCs were cultured on STO feeder cells with SSCs culture medium (SSCM), which is serum-free. SSCs began to proliferate on the second day and quickly formed gra...
Faheem Ahmed Khan1*, Sarzamin Khan2, Qurat-ul-Ain3, Saqib Ishaq1Muhammad Salman1, Abdul Rehman1, Ikram Ullah4, Kalsoom5 and Johar Jamil5
...ary to explore alternate methods for production of non-conventional proteins utilizing the available cheap sources. The indigenous Saccharomyces cerevisiae were isolated from different fruit samples, identified by conventional polymerase chain reaction (PCR) and were characterized for production of single cell microbial protein (SCMP). Out of 60 different fruit samples, a total number of confirmed S. cerevisiae isolates were 1, 2 and 1 from orang...
Małgorzata Dzierzęcka1, Sławomir Paśko2, Marcin Komosa3, Karolina Barszcz1, Bartłomiej Jan Bartyzel1 and Ewa Czerniawska-Piątkowska4*
...ible using other imaging methods. The angle between the coffin bone parietal surface and the ground was found to be correlated with age, with a 0.27 correlation coefficient at p=0.1, but not with body weight. The correlation coefficient for body weight was -0.08 at p=0.64.
...
Kamal Al-Samawi1, Mohamed Al-Hassan2, Hussein Migdadi3,4*, Megahed Ammar5 and Salem Alghamdi3
...23 post NM. These methods will provide early and precise detection of pregnancy compared to the routinely used serum P4 and US methods.
...
Muhammad Qayash Khan1,2, Muhammad Zubair Anjum2, Muhammad Adnan3, Abbas Khan1, Hafsa Zahid1, Javed Nawab4, Sher Zaman Safi5Muhammad Ishaq Ali Shah6, Atif Kamil7 and Abid Ali1*
...he traditional taxonomic methods.
...
Iram Liaqat1*, Tahir Hussain1,2, Aisha Waheed Qurashi3, Gulbeena Saleem2Asia Bibi4, Muhammad Fiaz Qamar5,ShaukatAli1 and Ikram-ul-Haq6 
...icken by three different methods i.e., congo red, test tube and air liquid interface coverslip, (2) biofilm quantification at different time intervals and (3) monitor antibiofilm effect of three proteolytic enzymes including trypsin, chymotrypsin and proteinase k against S. Gallinarum. We observed that S. Gallinarum has strong biofilm forming ability as observed by dark black colonies on congo red medium. Quantification assays such as test...
Saba Shabeer1, Riffat Tahira2 and Atif Jamal3*
 
...on in storage. Different methods including chemical, cultural, and biological control strategies are employed to control this fungus. In this review, mycotoxin production, characterization, identification, and different economically important diseases associated with Fusarium species as well as their control are discussed in detail.
...
Salman Ali1*, Ayaz Ali2, Riaz Ali Rind2, Majid Ali3, Zulfiqar Ali Mastoi2, Shagufta Naz3, Muhammad Shakir3, Rashid Ahmed Qaim Khani1
...aled that the cooking in methods were significantly varied (LSD<0.05) for different parameters. The fish cooked in microwave resulted average value of moisture 65.03%, titratable acidity 1.78%, pH value 6.26, protein 24.07%, fat 3.76%. Whereas, the fried fish resulted moisture 62.16%, titratable acidity 1.39%, pH value 5.96, protein 21.11%, fat 5.93%. The fish grilled resulted in moisture 60.19%, titratable acidity 1.32%, pH value 5.89, protein 20.28%, and ...
Muhammad Nawaz Kandhro1*, Nadia Mangrio1, Aijaz Ahmed Soomro1, Zia-ul-Hassan Shah2, Ghulam Sughra Mangrio3, Nihaluddin Mari4, Zulfiqar Ali Abbasi1 and Shazia Parveen Tunio5
 
 
...ion of suitable planting methods and NPK level play a major role in improving sugarcane yield and quality. Experiment was performed under field conditions at Sugarcane Research Institute, Tandojam, Sindh, Pakistan (located at 250 25.19 N 680 32.07 E) during 2016-17, repeated in 2017-18 and data was averaged. The sugarcane variety PSTJ-41 was used for this study. The experiment was laid out in spilt plot design. Main-plot consisted of planting
Hafiz Tassawar Abbas1*, Tamoor Khan1, Ghulam Khaliq2, Muhammad Aqeel Sarwar3, Muhammad Rashid4, Intazar Ali5, Muhammad Abuzar Jaffar2, Ghulam Ali Bugti6 and Muhammad Waseem4
 

...ological and biochemical methods of the host plants which help to prevent the extent of pathogen. 

...
Asma Chaudhary1, Qurat-ul-Ain Ahmad1, Afia Muhammad Akram1, Sadia Roshan2 and Javed Iqbal Qazi3*
...s well as disk diffusion methods with maximum growth inhibition zones i.e., 15 and 17 mm by Sphingomonas sp. AsCh-P3 and Bacillus aerius AsCh-A7 respectively. Six probiotic bacterial isolates of known enumeration (C.F.U.ml-1) showed growth antagonism for the fish pathogen with inocula comprising of less number of C.F.U.ml-1. Highest survival rates i-e 63.5 and 70.17% were expressed by Sphingomonas sp.AsC...
Suwati Suwati1, ErniRomansyah1,*, Syarifudin Syarifudin1, Yahya Jani2, Agus Heri Purnomo3
Damat Damat4 and Erkata Yandri5,6
...a-carotene. Right drying methods are needed to preserve the quality of dried seaweed. This study aims to compare the trend of weight reduction in seaweed during drying by natural and cabinet methods. The methods were used experimentally in the field and laboratory. And the data were analyzed using simple linear regression to formulate a trend of reduction in the weight of seaweed while dry...

Anwar Ali1,5*, Horia Shaukat2, Munir Ahmed3,5, Ahmed Bostani4 and Syed Ashiq Hussain3

...quately along with other methods. In spite of a wide exploration and study on electrical stimulation, the fact that it has been explored and investigated thoroughly in a number of countries around the world. It is currently applied properly on commercial scale in few countries. It has different impact on different type of muscles, so basic know how of changes occurring when it is applied to different kind of muscles should be known to get maximum benefits from...
Shehzadi Saima1, Maqsoda Perveen2, Muhammad Nawaz1, Khalid M. Ahmad1,* and Haider Sultan1
...s by different treatment methods, and to find out which method is more promising. The corms of Gladiolus hybrid cv. yellow stone was used for plantation. Experiment was carried out in complete randomized design (CRD) with four replications and three fertilizer treatments (control, 2g/pot with side dressing, 2g/pot with foliar spray). Results revealed that foliar spray of potassium enhanced most of parameters i.e. length of stalk, fresh weight of stalk, ...

Aamer Sattar1, Sadia Sultana1*, Abid Niaz1, Muhammad Aftab1, Ghulam Sarwar2, Irfan Rasheed3, Muhammad Shoaib4, Raheela Naz1, Amina Kalsom1, Nisa Mukhtar1, Farah Rasheed1, Arfan ul Haq1, Munazza Rafique5, Muhammad Arif1, Sarfraz Hussain1 and Jafar Salim6

...rice with 3 major plots (methods of residues management) i. traditional practice by complete removing (C) whole crop biomass from field, ii. burnt (B) practice by burning the crop biomass in field after threshing and iii. Residue’s (R) incorporation practice by rotavating the crop biomass into the soil. The selected field was sufficient in exchangeable K and available P but was deficient in organic matter contents. The results showed that in first year t...

Syed Mazuan, Syed Mohamed, Insyirah Ishak, Dzolkhifli Omar and Norhayu Asib*

... plana. Various spraying methods had been developed for controlling the bagworms. A ground study was conducted in FELDA Gunung Besout 04, Perak, with its main focus on mistblower application towards bagworms. Stihl SR420 mistblower was characterised by having 1.2L / min flow rate, produced volume median diameter of 80μm, and achieved spraying productivity of 2.58 hectares’ land size (approximately 350 oil palms) per man day. The study was to evaluate ...
Aneeqa Saleem1, Muhammad Islam1, Hamid Saeed1* and Mehwish Iqtedar2
...ained using hot and cold methods. Ethylene glycol (0.75% V/V) was used IP for 10 days to develop mice model of nephrolithiasis. Extracts were administered at a dose of 400 µg/g for 11 – 21 days. Outcome measures included serum creatinine, uric acid and BUN levels. Significant differences exist with regards to primary and secondary metabolites between both peel and pulp extractives. After 21 days of treatment in mice, only chloroform (CHL) extractiv...
Fuhua Zhang, Yishuang Yu and Shibao Wu*
...d with hormone detection methods. Efficient detection methods such as B-ultrasonography are critical to avoid housing already-pregnant pangolins together with males, which may mate with the females again or stress them, resulting in abortion. This approach also helps to conserve male reproductive resources, thereby improving the reproductive efficiency of captive Sunda pangolins. Effective and efficient captive breeding prog...

Aioub Sofizadeh1, Ehsan Allah Kalteh2, Shahin Saeedi3, Mulood Mohammadi Bavani4*

...rolling and ultra violet methods from May to September, 2019. Then, the specimens were put into a 75% alcohol-containing plastic bottle. Finally, the specimens were identified using a valid identification key. Distribution maps were prepared using ArcGIS (Ver 10.4.). A total of 111 scorpion samples were captured. All the samples belonged to the Buthidae family, including Mesobuthus eupeus (97.3%), Orthochirus farzanpayi (0.9%), and Mesobuthus caucasicus (1.8%)...

Imtiaz Ahmed*, Naveed Ahmed, Abdul Waheed, Muhammad Abbass Khan, Noorullah Khan and Fayyaz Ahmed

...“Impact of growing methods and direction of sowing on the growth and seed production of ridge guard (Luffa acutangula Roxb)” was conducted at PARC-National Tea and High Value Crops Research Institute, Shinkiari, Mansehra, Pakistan during the months of April-September, 2019. The experiment was laid out in Randomized Complete Block design (RCBD) with split plot arrangement having three replications. There were 45 plants in each replication. The treat...

Muhammad Ibrahim Kubar1, Fahad Nazir Khoso1*, Imran Khatri1, Niaz Hussain Khuhro2 and Arfan Ahmed Gilal1

...e the farmers to use IPM methods for healthy vegetable with high yield.

...

Amar Iqbal Saqib, Khalil Ahmed*, Muhammad Khalid Bhatti, Ghulam Qadir, Muhammad Qaisar Nawaz, Muhammad Ashfaq Anjum, Abdul Rasul Naseem, Aftab Ahmad Sheikh and Belqees Akhter

...acticable and economical methods in two different textured soils. Treatments were: (A) Types of soils. 1) sandy loam, 2) clay loam, (B) Remedial strategies, 1). canal water, 2) saline-sodic tube well water (continuous), 3) three irrigations of tube well water and two of canal water in a cyclic manner (short cyclic use), 4) tube well water + gypsum @ RSC of water, 5) tube well water+ canal water in 1:1 ratio (conjunctive use). Data showed that the highest bioma...

Arshad Khan1, Mohammad Ihsan1, Mohammad Nisar1, Ali Hazrat1*, Murad Ali3, Rashid Ul-Haq3, Khalid Khan2, Karishma Gul1 and Shah Faisal1 

...igated through different methods such as morphological traits, biochemical (SDS PAGE) and molecular markers. The present work was conducted on the basis of morphological and biochemical characterization in order to estimate the genetic diversity among the barley landraces; to explore significant variation which can be used in breeding programs. For this purpose, 40 barley landraces were collected from Dir Lower and Swat, districts of KP, Pakistan. A total of 1...

Iftikhar Jan* and Sahib Alam

...ction) and immunological methods i.e., enzyme linked immunosorbent assay (ELISA). All the four cultural methods successfully differentiated aflatoxigenic and nonaflatoxigenic isolates, however ammonium hydroxide vapor induced test was found to be the most efficient (80.29%) for segregation of the isolates. Among all isolates, thirty were screened for their total aflatoxin production in corn meal agar medium by using ELISA te...

El-Habbaa, A.S.

...ere
suitable methods for CPDV detection but PCR was most specific and sensitive
diagnostic tool for rapid detection of CPDV.
...

Hanaa A. Ahmed1 and Eman M. AboHatab2

...ed positive by molecular methods. Eleven out of twenty one nodular samples were positive by (IFAT) in CAM, which prove that PCR and Real Time PCR are much sensitive and rapid diagnostic tool of LSD reflecting their importance in controlling the rapid spread of disease in Egypt.

...
Eman A. Ahmed1, Osama Y. shalaby2, Emad F. Dwidar2, Samah A. Mokbel1 and Ahmed K. El-Attar1
...e secondary walls. Three methods were done towards the production of phytoplasma-free tomato plantlets through tissue culture using antibiotic compound (tetracycline hydrochloride in three concentrations 25mgL-1, 50mgL-1 and 75mgL-1), irradiation by three doses (3, 5 and 10 Gy) of gamma ray and natural compound (1ml of garlic juice). All treatments proven to be a very useful effect against phytoplasma, except concentration 25 mgL-1 of tetracycline hydrochlorid...

Lamya A. F. Ateya1, Said A. Ahmed 2, Mansour H. Ayman3, Khamees K. Ashraf 4, Heba A. Abdel-Hady 5

Ola Youssef 1; EL-Deeb A.H.2 Nassif S.A.1 and Ahmed A. El-Sanousi 2.

Ehab M. El-Nahas1, Hemat S. El-Sayed2, Sawsan S. El-Basuni3, Gabr F. El-Bagoury1

Ayman S. El-Habbaa 1, Gabr F. El-Bagoury1, Samar F. El-Adaway2, and Susan S. El-Mahdy2

Manar F. Seioudy1, Magda M. Sayed1, Ahmed A. El-Sanousi2 and Mohammed A. Shalaby2

Muhammad G. Khodary1, Ayman H. El-Deeb2, Mohamed M. Emara2, Othman E. Othman1, Hussein A. Hussein2

Lamiaa M. Omar, Mohamed A. Abdrabo, Dali M. Omar, Nermeen A. Marden

...ation (VI) and titration methods
in (ECE) or by molecular assay for detection AI virus specific nucleic acids such as (rRT-PCR).
Methods: Titration of the HPAI virus for infection of SPF chicken. The tracheal swabs were collected
from diseased chicken for virus isolation and quantification by ECE inoculation and rRT-PCR.
Results: the titer of original HPAI virus was 1010.3 EID5...

Ahmed A. Azab, Wesam H. Mady, Ali Zanaty, Mahmoud Samir

...n of different isolation methods was done
by generating a standard curve by real time PCR.
Results: The results revealed that the propagated virus in CEK showed the highest virus titer (5.952 x
106 and 3.245 x 106) than Vero cell line (7.184 x 101 and 2.14 x 103) and ECE (7.536 x 103 and 5.444 x
105) after both 48 and 72 hours respectively.
Conclusion: The chicken embryo kidney cell (CEK) is the most ...
Ashraf S. Khameis, lamya F. Atteya, Ayman H. Mansour, Heba A. Abdelhady, Ashraf A.
Saad

Dina A. Abdulrahman1, Ayman H. EL-Deeb2, Momtaz A. Shaheen1 & Hussein A. Hussein2

Dalia M. Omar1, Nermeen A. Marden1, Lamiaa M. Gaafar1, Elham A. El-ebiary1, Khalid El-Dougdoug2, Badawi Othman2, Abd Elsattar Arafa3, Hussein A. Hussein4

Nermeen A. Marden1, Lamiaa M. Gaafar1, Hussein A. Hussein2

...ironment. The evaluation methods of the AI vaccines are very important
for controlling the license of the bad vaccines. Comparative studies between the invitro and invivo
methods for 8 types of AI vaccines (5 batches for each) to evaluate their efficacies were done.
Methods: The invivo method, was done through the vaccination-challenge pathway of SPF chi...
Sahar Mubashar*, Tariq Mukhtar and Nasir Ahmad Khan
...ngi and viruses. Various methods are used for the detection of the virus such as nucleic acid and immunological methods but RT-PCR is considered as the most reliable. Some antiviral drugs have shown to be effective against the virus like Favilavir, Remdesivir, Chloroquine, hydroxy-chloroquine, Tocilizumab etc. but further clinical studies are required to confirm their efficacy. In Pakistan, blood plasma therapy is in high de...

Akram I. Aboelkhair1, Ayman H. El-Deeb2, Momtaz A. Shahin1 and Hussein A. Hussein2

Nagwa K. Meselhy1, Mohamed A. Abo El-khair1, Basem M. Ahmed2, Sherif A. El-Soally3,
Abdelhamid M. Fares1, Mohamed A. Nayel1 and Hussein A. Hussein2

Hanaa A. Elsamadony1, Laila A. Tantawy1, Sabry E. Omar2 and Heba A. Abd Alah1

...>
Egypt to improve methods of virus control and to usually determine the relationship of circulating
CAV with vaccine strains and other CAV strains. The most obvious thing in this study, that’s we need
to make our vaccine from our strain which circulating in the field with periodic updating.
...
Othman N. O. Mansour 1, Naglaa Hagag 2, Momtaz A. Shahein 1, Sayed S. Hassan 1 Ahmed
A. EL-Sanousi 3 and Mohamed A. Shalaby3

Rana A. Rabiea1, Mohamed Fawzy2, Mohamed H. Khodeir3 and MokhtarM. EL-Tarabili2

Mohsen M. Elsharkawy1, Salem H. Homayed1, Mohamed M. Elsawy2 and Amr A. Khedr1

Allam A. Megahed, Badawi A. Othman, Samar S. El Masry, Abeer A. Faiesal and Mohamed A. Nasr Eldin
Samira M. Bolis1, Magda Mahmoud2, Salah E. Gumma3, Naser E. Bilal1 and
Isam ElKhidir4
Riham H. Bassam, Hussein A. Hussein, Mohamed M. Amer, Basem M. Ahmed and Ahmed A. El-Sanousi

Riham H. Bassam, Hussein A. Hussein, Mohamed M. Amer and Ahmed A. El-Sanousi

Aml S. El-Saadany1, Ayman H. El-Deeb2 and Hussien A. Hussien2

Mohamed F. ElKersh1, Fatma M. Abdalla2, Gamilat K. Farg2, Soad A. Nasef3, Ahmed A. Ali2

Mohsen M. Elsharkawy1, Sara E. Hanbal2, Samir A. Sidaros1, Mohamed M. Elsawy2, Ali H. Hamed2

Shimaa A. Khalaf1 , Mohsen M. Elsharkawy2, Samir A. Sidaros2, Shawky A. El Kewey2, Ayman F. Omar2, Ali Hamed1

Huda S. Darwish 1, Om-hashem M. El-Banna2, Mohamed S. Abbas3, Hoda M. Waziri 1, Maisa A. Awad1
Hadeer M. Mossa, Ausama A. Yousif, Emad A. Aboelsoud, Mahmoud El Gamal, Ahmed El-
Sanousi
...faster and more accurate methods to
analyze, characterize and qualify the virus as a seed for future vaccine production. Analysis workflow
starts on a high performance laptop device (GENUS), sequence data is generated for bacteria, viruses,
fungi and archaea that present in the sample it was then classified to subspecies and strain level in a
quantitative manner in approximately 48min and 23 second.
C...
Ahmed A. ElWakil1, Ausama A. Yousif2 , Adel A. Fayed3, Ibrahim M. elsabagh2, Mahmoud
El Gamal2, Omnia H. Refaei3

Mohamed M. Mashaly1, Ayman H. El-Deeb2, Momtaz A. Shahein1, Hussein A. Hussein2

Soad, E. Elsayed 1, Mohammed A. Shalaby2, Ausama A. Yousef2, Abu bakr A. Agor1,
Sherouk E. Aly1
Shourok E. Aly 1, Karim Zaki1, Soad E. Elsayed2, M. Elgamal2, Ausama A. Yousef1, Mohamed A. Shalaby1

Ljupco Angelovski*, Zagorka Popova, Katerina Blagoevska, Sandra Mojsova, Marija Ratkova Manovska, Mirko Prodanov, Dean Jankuloski, Pavle Sekulovski

...ltiplex PCR method. Both methods confirmed the overall prevalence of C. jejuni of 39.2%. In the second part of the study 108 confirmed isolates of C. jejuni were analyzed for the presence of resistance genes (CmeB, Blaoxa-61, tet(O), aph-3-1 and aadE). The analysis in the third part of the study was concentrated on the antimicrobial resistance of the C. jejuni isolates towards three important antimicrobials (ciprofloxacin, erythromycin and te...

Mian Ibad Ali Shah*, Yasir Saleem Afridi and Laiq Hasan

...is comprising of various methods and factors have been used to infer conclusions from data. Some important factors like, stringency index, number of tests, etc. have been utilized in this paper to achieve the aforementioned goals. 
...

Iftikhar ud Din* and Yousaf Hayat

... widely used statistical methods for assessing the differences among the means of more than two treatments by considering single trait independently. In reality these traits are not independent and appropriate techniques for analyzing multiple traits simultaneously, is the multivariate analysis of variance (MANOVA). This study deals with the illustration of MANOVA using simulated data, related to agricultural trials. The appropriate design,

 Yongmin Li1,2, Huabin Zhang1, Xiaoyou Wu1, Dongwei Li 2, Peng Yan1 and Xiaobing Wu1*

... Bayesian inference (BI) methods supported the sister-group relationship between ((Rhacophoridae + Mantellidae) + Ranidae) and Dicroglossidae. Within Rhacophoridae, two species of the genus Rhacophorus (R. schlegelii and R. dennysi) were clustered together with the representative of the genus Polypedates (P. megacephalus), meanwhile, the representative of the genus Buergeria (B. buergeri) occupied the basal posi...
Houqiang Luo1*, Yanfang Lan2, Ping Gan3, Wenjun Zhou4, Meng Wang1
Bing Hu5, Zhuning Zhang5, Yu Bai1* and Kun Li6*
... and molecular detection methods were used to identify the tick species and CME by amplification of the cytochrome oxidase subunit I and disulfide oxidoreductase gene, respectively. The results indicated that 1.29% of the serum samples were positive for E. canis, and 5.50% of dogs were infested with ticks. The Wenzhou samples of R. sanguineus exhibited a high homology (99.7%–99.8%) and these parasites showed a 99.1%–100% homology to p...

Sana Javeid* and Khalid Nawab

...gents, extension contact methods, strategies and trainings showed significant relationship with poverty. Hence, it is recommended that efforts are needed to regularize all extension activities for poverty reduction in the study area.

...

Kawa A. Ali1*, Sazar S. Noraldeen2 and Arshad A. Yaseen2, 3

... first by nondestructive methods using SPAD (SPAD-502 Plus Konica Minolta Tokyo, Japan) and atLEAF chlorophyll meter (at-LEAF FT-GREEN-LLC Willington, DE, USA) instruments, then the same leaves were cut and chlorophyll estimation were done by destructive methods using two organic solvents ( 80% acetone and 100% ethanol). Results demonstrate significant differences between SPAD and atLEAF data for tomato and pepper plants whe...
Chaochao Hu1,2, Xue Xu1, Wenjia Yao1, Wei Liu3, Deyun Tai1, Wan Chen4 and Qing Chang1*
...s by at least one of the methods. This study provides a valuable resource facilitating further study of phylogenetic and evolutionary analysis for wader, and improves our understanding of the evolutionary and taxonomic research within Charadriiformes.
...
Muhammad Bilal Tayyab1, Muhammad Zeeshan Majeed1*, Muhammad Asam Riaz1, Muhammad Anjum Aqueel2, Sylvain Nafiba Ouedraogo3, Muhammad Luqman4, Kanwer Shahzad Ahmed1 and Mujahid Tanvir1
...ip and leaf-dip bioassay methods, respectively. Results of initial screening bioassay showed the highest mortality of D. citri by 10% extracts of Mentha longifolia (L.) Huds. (93%), Melilotus officinalis (L.) Pall. (91%), Nerium indicum Mill. (89%), Datura alba L. (88%) and Salvia officinalis L. (81%). Second bioassay conducted against A. gossypii using different concentrations (5, 10, 20 and 40%) of the most effective botanical extracts revealed that the extr...

Hafiz Muhammad Tahir*, Iram Liaqat*, Junaid Nadeem, Hammad Aamir and Shaukat Ali

...ial by either of the two methods. However, application of web silk using the DSS treated discs showed no growth inhibition compared to the DgS treated discs, where increased inhibition zones were observed against all three bacteria. This study suggested that spider silk does possess antibacterial potential, however, this is not an intrinsic property and is type specific as well as species specific. Use of spider silk for coating of medical devices such as sten...

Usman Asghar1*, Muhammad Faheem Malik1, Umer Rashid2, Naeem Mahmood Ashraf2, Sumera Afsheen1 and Muhammad Hashim1

...DNA-based identification methods are ideal and reliable. DNA was extracted from muscle tissues of cow, buffalo, goat, donkey and a dog. A set of degenerative primers was designed for Polymerase Chain Reaction (PCR) of the cytochrome b gene fragment. All PCR was accomplished at the same reaction conditions. Amplified products had a length of 400 bp, which was our estimated length. After the sequencing of these amplicons, In silico restriction analysis was perfo...
Farhat Iqbal1, Abdul Waheed2*, Zil-e-Huma3 and Asim Faraz2
...radient boosting machine methods have been used to predict the live body weight (target variable) of the animal. The predictors (independent variables) included in the current study were sex of the animal, neck length, diagonal body length, head girth above eyes,shank circumference, belly sprung, and rump height measurements of beetal goats (1 – 7 years in age) of Pakistan. In order to test the performance of candidate methods

Mohammed Abdel-Mageed Abdel-Aziz Abdel-Mageed1, Eman Alsayed Hammad2*, Nashaat Abdel-Aziz Mahmoud1 and Anas Farag El-Mesalamy1

...atodes better than other methods of application.

...

Mst. Deloara Begum1, Md. Muniruzzaman1, Md. Salauddin2* and Md. Mostafizer Rahman1

...cteriological laboratory methods and purified isolates were identified according to gram’s staining reaction, colony morphology, cultural characteristics, biochemical and antibiotic susceptibility test. All most 100% dried and 20% cooked fish sample were contaminated. In this study seven different species and 168 isolates were identified from dried fish and these were Escherichia coli 21.43% (36), Vibrio spp. 18.45% (31), Staphylococcus spp.17.86% (30), ...

Ayesha Khan1*, Asmatullah2, Urooba Perviaz1, Khalid Nawab1 and Mahmood Iqbal1

... fertilizers alternative methods are adopted to decrease use of fertilizers. One of the techniques for organic farming is EM-Technology. EM-Technology is used in addition with animal manure and other organic wastes effectively in agriculture. The universe of the study was Malakand division of Khyber Pakhtunkhwa. The EM-Technology using 128 farmers’ data were gathered from the District Director Agriculture of six districts. Each farmer was interviewed fac...
Misbah Sarwar1*, Abdul Hamid2 and Iftikhar Hussain2
...s employed both of these methods to capture cranes. In total, 7947 cranes were captured during the study period. Age wise segregation showed that 6624 (83.4%) of captured cranes were adults and 1323 (16.6%) sub-adults. Among the adult cranes, 3279 (49.5%) were males and 3345 (50.5%) females. Cranes captured during spring and fall from 2012 to 2015 included 1021 and 502; 981 and 527; 787 and 311 and 843 and 462 respectively. The difference between the spring an...

Eman H. Mahrous1, Mohamed. W. Abd Al -Azeem2, Faisal A. Wasel1, Waleed Younis2* 

...t and other conventional methods of identification. Recovery of P. multocida isolates of diseased rabbits from lung, liver, and heart were 23 (46%), 11 (22%), 13 (26%), respectively, with a total incidence of 47 (31.3%). Biochemical reactions proved that 20 (42.5%) out of 47 isolates had the typical biochemical properties of P. multocida. However, only 10 isolates were identified as P. multocida using species-specific primers by conventional PCR with an incide...

Muhammad Zeeshan Majeed1*, Muhammad Irfan Ullah1, Dilbar Hussain2, Muhammad Luqman3, Muhammad Qasim1, Gulfam Yousaf1, Hamza Latif1 and Muhammad Zeeshan1

...ilter paper-dip bioassay methods were used against sap-feeding pests and termites, respectively. Factorial analyses of variance revealed a significant impact of tested insecticidal formulations and exposure time on the mortality all insect pest individuals. The most effective differential chemistry insecticides against mango leafhopper, citrus psyllids, mango mealybugs and subterranean termites were nitenpyram, clothianidin, thiamethoxam and imidacloprid (caus...

Abbas M. Al-Azab1*, Ahmed A. Zaituon2, Khalid M. Al-Ghamdi3, Fahd M. Abd Al Galil4,5 

...(black holes) and manual methods were used for collecting adults and immature stages in five residential areas in Jeddah. A total of 796 adults of Aedes aegypti mosquitoes were collected. Out of these, 373 males (46.8 %) and, 423 females (53.2%). Out of 950 collected immature stages, 83.41% larvae and 16.6% were pupae. The obtained statistical analysis showed that the availability of the adult and juvenile stages of A. aegypti occurred throughout the year in d...

Ijaz Rasool Noorka1,2*, Bilal Ahmad Khan3*, Kafeel Ahmad4, Zafar Iqbal Khan4, Muhammad Ather Nadeem3, Amer Nawaz2, Tasneem Ahmad5 and Humayun Bashir4

...dard samples preparation methods were worker to check occurrence of the heavy rock’s metals in the forage and soil samples. The Atomic Absorption Spectrophotometer was used to check Iron (Fe). The results revealed that Fe were found in safe limit, as per WHO. 

...
Amjad Ali1, Muhammad Amir2*, Bilal Ur Rehman2, Zafar Khan3 and Abid Munir1
 
...ally, appropriate proven methods are used for processing fingerprints in a sequential manner followed by the application of various encryption algorithms for ensuring protection of such biometric data. In fingerprinting, image processing techniques include: low-quality-fingerprint enhancement, normalization, binarization and thinning of fingerprint image followed by feature extraction. In this paper, a novel encryption method for minutiae based low-quality-fin...

Mustafa Ozan Atasoy

...een generated by various methods and some of these constructs have been made commercially available so far. The efficacy of HVT-vectored vaccines has been assessed either individually or in combination with other vaccine formulations; hence, the optimal competence of these vaccines against viral diseases has been reported. Based on the current knowledge, this article outlines the benefits and drawbacks of HVT-vectored vaccines in combating health-threatening p...

Mirza Gul1*, Muhammad Zahid1, Ikram Ilahi2, Hazrat Ali2*, Fida Hussain3 and Muhammad Anwar Sajad3

... performed following WHO methods, with slight modifications. LC50 values for A. scoparia and A. indica against early fourth instar larvae were 360.4 and 971.1 ppm, respectively. LC50 values for pupae were 1665 and 2838 ppm for A. scoparia and A. indica extracts, respectively. Percent knockdown after 1 h exposure was 49.0 for A. scoparia. KDT50 and KDT90 values for A. scoparia were 69.7 and 763.5 min, respectively. LC50 values for A. scoparia and A. indica agai...

Khadim Hussain Memon1,*, Amir Mahmood Memon2, Tahira Jabeen Ursani3, Abdul Manan Shaikh1 and Sapna Waheed Memon1

...th function and ELEFAN-I methods to estimate the growth parameters. The asymptotic length (L∞) and the growth rate (K) were 82.95 cm and 0.390/year, respectively, wherein the hypothetical age at zero length (t0) and goodness of fit or score (Rn) -0.495 year and 0.561 were also estimated correspondingly. The annual total mortality (Z) was 1.03/year, natural mortality (M) was 0.635/year at mean annual surface water temperature of Indus River 21°C, fish...

Irshad Ahmad1,2*

... adopting the regulatory methods of domestic and international quarantine. This paper provides updated information about IPM, which look like a promising paradigm that can be adopted for the control of red palm weevil.

...

Yuan Zhang1, Qingyue Han1, Peiquan Du1, Yi Lu1, Lianmei Hu1, Sadaqat Ali2, Khalid Mehmood2, Sajid Hameed2, Zhaoxin Tang1, Hui Zhang1* and Ying Li1*

 

...ntroduces the diagnostic methods of IHC and immunohistochemistry, rarely used in the diagnosis of lymphoma in clinical practice in China, which can provide a reference for veterinarians in clinical diagnosis.

...

Yuan Zhang1, Qingyue Han1, Peiquan Du1, Yi Lu1, Lianmei Hu1, Sadaqat Ali2, Khalid Mehmood2, Sajid Hameed2, Zhaoxin Tang1, Hui Zhang1* and Ying Li1*

 

...ntroduces the diagnostic methods of IHC and immunohistochemistry, rarely used in the diagnosis of lymphoma in clinical practice in China, which can provide a reference for veterinarians in clinical diagnosis.

...

Neven Waheeb1*, Sherif Marouf2, Essam Nasr3, Shaymaa Abdelmalek2 

...nvasive and non-invasive methods. Invasive methods require endoscopy which is uncomfortable and unacceptable for most patients. Therefore, non-invasive methods particularly serological tests are easier and comfortable for patients. The objective of this study was to comparatively evaluate noninvasive enzyme-linked immunosorbent assay (ELISA) and polymerase chain reaction (PCR) in the diagn...

Lin Huang1, Ling Mai2, Keyan Zhong1 and Xinjun Chen3*

...modification sites. Both methods predicted that SeFABP contained 5 linear epitopes, and were highly coincident, indicating that SeFABP had good immunogenicity. Its secondary structure contains 2 α-helix (13.07%), 10 β-sheet (56.92%) and 30% random coli. The comparison of multiple FABPs sequences showed that the species were identical and highly conserved at multiple AA sites. Specifically, SeFABP has the highest similarity with T. multiceps and M. v...

Abdul Jabbar*, Anees-Ul-Hussnain Shah, Abdul Basit, Ghulam Ahmad, Aftab Ahmad Khan, Suleman Raza, Muhammad Sultan Ali Bazmi, Imtiaz Akram Khan Niazi and Ahmad Hussain

...ence of different sowing methods on seed production of berseem (SB-11) was directed at Fodder Research Institute, Sargodha, Pakistan during 2017-18 and 2018-19. Different sowing methods, broadcast and row spacing of 15cm, 30cm, 45cm, 60cm apart were used. Plant height (cm), number of capsules/m2, number of seeds/capsule, 1000-grains weight (g) and seed yield (kg/ha) parameters were studied. It was observed that sowing
Syed Makhdoom Hussain1,*, Hina Gohar1, Muhammad Asrar1, Muhammad Mudassar Shahzad2, Azhar Rasul1, Majid Hussain3, Muhammad Zubair ul Hassan Arsalan1, Nisar Ahmad4 and Aqsa Sharif1
...etermined using standard methods and formulae. Highest minerals absorption (Ca, Na, K, Fe, Cu, P, Mg and Al) was observed in the fish fed at 400mg/kg of polyphenols in canola meal based diets. Similarly, best hematological parameters (RBCs, WBCs, PLT, Hb, PCV, MCHC, MCH and MCV) as well as proximate composition (crude protein, crude fat, ash, moisture and carbohydrates) were noted in fish group fed on 400mg/kg of polyphenols in diet. Hence supplementation of p...

Juliet N. Ozioko1, Obinna V. Ayogu2, Benjamin O. Ezema1, Dilibe C. Uramah3 and Kingsley O. Omeje*,1

...uantified using standard methods. Alkaloids, flavonoids, tannins, saponnins and terpernoids were detected. The leaf extract had 0.415 % of alkaloids, 0.102 mg/TAEq of tannins, 0.211 mg/QEq of flavonoids and 0.360 mg/GAEq of phenolics. Phytol, oleic acids, hexadecanoic acids and octadecanoic acids were detected in the leaf extract by Gas Chomatography-Mass Spectroscopy. White blood cells, hemoglobin and platelets were some of the hematological indices increased...

Raheela Naz1*, Muhammad Aftab1, Ghulam Sarwar2, Ana Aslam1, Qudsia Nazir1, Asifa Naz3, Abid Niaz4, Farah Rasheed1, Amina Kalsom1, Nisa Mukhtar1, Sadia Sultana1, Ifra Saleem1, Arfan ul Haq1, Muhammad Arif1, Aamer Sattar1, Sarfraz Hussain5 and Muhammad Adnan Rafique6

...t of fertilizer applying methods for nitrogen and potash at different times was investigated on wheat crop. Wheat was sown as a test crop with six different fertilizer application methods at varying times under RCBD arrangement with three replications. The experimental soil was high in pH, low in fertility status and free from salinity and sodicity hazards. Wheat grains were analyzed for mineral contents. The results reveale...

Alexander Tsybulsky1*, Tatyana Tabakaeva1, Anna Klimovich2, Michail Shchelkanov1, Eduard Kostetsky1, Maksim Aliev1, Anton Degtyarenko3 

...in cats. The fundamental methods for such pathologies diagnostics, along with haematological analysis, include the replicative and expressive activity of FeLV evaluation in the infected organism cells, innate immune factors for antivirals evaluation, interferon system (IFN) in particular, molecular guard factors for cell genome integrity evaluation (p53, etc.). Methods. In domestic cats with a previously established diagnosi...

Saba Iqbal, Asmat Ullah*, Muhammad Luqman, Hafiz Muhammad Akram, Muhammad Kashif Munir and Nawal Zafar

...d broadcast (0.05 kg/m3) methods. Moreover cost benefit ratio of ridge (1:1.15) and bed (1:1.25) planting techniques was higher than drill (1:0.93) and broadcast (1:0.44) techniques. In conclusion, it is recommended that wheat may be planted on ridges and raised bed to have more yield, high water use efficiency and sound financial return to farming community.

...

Amtul Jamil Sami*, Sehrish Bilal and Syeda Anum Zahra

...s of semen supply or the methods used for its introduction can give clues about these findings.

...
Anjum Ara1, Usman Ali1, Muhammad Furqan2*, Zulfiqar Ali2
Muhammad Mudassar Shahzad3, Khawaja Basharat Ahmed4
Riaz Aziz Minhas4, Waqar Ahmed2, Zakir Hussain2 and Mian Aman Ullah2
...rvey, tracking, trapping methods were applied to collect data related to diversity, distribution, density and questionnaire method applied to assess threats. Analysis of data revealed that four freshwater turtle species including Indian flapshell turtle (Lissemys punctate), Indian narrow headed softshell turtle (Chitra indica) Indian softshell turtle (Nilssonia gangetica) and Crowned river turtle (Hardella thurjii) were identified and distributed in all study ...

Riaz Shah1*, Shaikha Hilal Al Ismaili2, Sheikha S. Al-Siaby1, Amal Mohammed Al Nasiri2, Thuraiya Hafidh Al Maskari2, Jamal AlSabahi3 and Huda Al-Ruqaishi3

...ng conditions and drying methods of basil may change its essential oil quantity and composition. The aim of this study was to determine the chemical composition of essential oils extracted from commercially available fresh and dried organic or conventionally grown basil available in Oman. The essential oils from Omani and Indian fresh conventionally grown basil, fresh organic Belgian basil, dried organic Egyptian basil and dried conventionally grown UAE basil ...

Muhammad Tahir Jan1, Sarfraz Ali Shad2, Mushtaq Ahmad Saleem3 and Muhammad Binyameen2,*

...ing insect pest scouting methods to determine the potential damage and to apply control measures for key pest like Earias vittella in cotton. Field experiments were conducted to assess the distribution of E. vittella eggs within the cotton plant. Out of 240 plants inspected, E. vittella females laid 204 and 194 eggs on 40.9% and 36.9%of the plants in 2011 and 2012, respectively. Spearman Rank Correlation showed that a significant and positive relationship (ass...

Semra Okur Gumusova1, Gul Fatma Yarim2, Cuneyt Tamer1,*, Ayris Salt2 and Gokhan Inat3

...y the spectrophotometric methods. Data obtained revealed higher MDA levels in IPNV infected cells than negative control medium (p<0.001). However, SOD and GPx levels in IPNV infected cells were lover than negative control cells medium (p<0.001). These results indicate that, cell damage in RTG-2 cell line, that is caused by IPNV is assosiated with oxidative stress.

...

Fatma Desouki Mohammed Abdallah

...role in many statistical methods like principal component regression.
 
Keywords | Carcass traits, Eigen-values, Japanese quail, Principal component analysis, Scree plot
...
Alshimaa A. Hassanien1*, Asmaa Osama Tolba2, Asmaa A. A. Hussein3, Walaa M. Elsherif4
...for the best application methods.
 
Keywords | S. aureus, Meat products, Food handlers, 23S rRNA sequencing, Casein, α-lactalbumin
...

Hazel Tamakan* and Huban Gocmen

... by conventional culture methods, automated identification system and PCR-RFLP. This is the first study to research the presence of MRSP in cats and dogs in Cyprus. The PCR-RFLP method based on the pta gene was confirmed to S. pseudintermedius. Methicillin resistance results were revealed with VITEK 2 and mecA gene detection was performed by PCR. A total of 100 samples including 50 cats and 50 dogs were examined from 6 veterinary clinics. S. pseudintermedius (...

Tarık Bugra Saruhan and Alptug Sarı*

...and indirect observation methods were used in field studies. Direct observations were made by one or two-person using point and line observations, as well as using camera traps. In indirect observations, signs such as the jackal’s footprints, scats, etc. were used. As a result of the research, data such as 253 camera trap images of the jackal, direct observation in one area, 16 jackal howling sounds in three areas, and footprints in one area were obtaine...

Hilarius Yosef Sikone1*, Budi Hartono2, Suyadi2, Bambang Ali Nugroho2 

... of the sample using two methods: purposive sampling (applied to farmer respondents) and snowball sampling (to collect respondents from traders and butchers). Samples used in the study were 115 respondents consisting of 90 farmers, ten collectors, four inter-island cattle traders, six cattle butchers, and five meat retailers. The data was analyzed quantitatively, the presentation of the data was descriptive, and the added value of marketing was calculated usin...

Heba El-Zahar*, Zeinab Abd El-Rahman, Abbas El-Naggar 

...imple and cost-effective methods of diagnostic and clinical assessment are urgently required. The goal of this study was to look at the hematobiochemical alterations in association with the evaluation of C-reactive proteins (CRP), haptoglobin and fecal calprotectin concentration as prognostic markers in dogs with IBD. After a detailed clinical, laboratory and ultrasonographic examination 21 IBD dogs with symptoms of chronic gastrointestinal diseases were chose...

Naila Shahzadi1, Hafiz Muhammad Tahir1*, Shaukat Ali1, Muhammad Farooq Bhatti2, Azizullah1, Shafaat Yar Khan3, Abdul Khaliq4

...lated to sericulture and methods to improve economic and biological traits through diet supplementation have been discussed.
...

Ji Xu, Zhonghua Liu, Zhenhai Cui and Wenhai Zhao*

...ression level. The above methods were used to detect the effects of miR-99a overexpression on IL-1β-induced chondrocyte inflammatory factors and apoptosis rate. Anti-miR-NC and anti-miR-99a were transfected into chondrocytes, respectively, and treated with a culture solution containing GSTT and IL-1β for 24 h. Inflammatory factor levels and apoptotic rates were detected. After IL-1β treatment, the levels of inflammatory factors IL-6, TNF-α...

Husmaini1*, Riza Andesca Putra1, Indri Juliyarsi1, Tevina Edwin1, Linda Suhartati1, Aditya Alqamal Alianta1, Harmaini2 

...ed sources, and hatching methods in maintenance management. There were cuts in KBC 59.65% with 277 chickens and sales of KBC 64.91% with 397 chickens, It concluded that the increase in the KBC population in in-situ areas is still slow, so it is necessary to improve maintenance management strategies in order to maintain indigenous chicken Indonesian especially in West Sumatera.

Keywords | Kokok Balenggek Chicken, Population Structure, Management, in-sit...

Rana A. Ali1, Abd-Elraheim A. Elshater2, Heba A. Mohammed3*, Mahmoud Elshazly4 

Mashakgene Isaac Senoamadi, Thobela Louis, Tyasi Teedzai Chitura* 

...linical haemonchosis and methods of controlling Haemonchus contortus and other internal parasites in a communal farming system of Limpopo province, South Africa. Twenty-six sheep and one-hundred and sixty-three goats were evaluated for clinical haemonchosis using the FAMACHA© diagnostic system. Information on the methods of control used by the smallholder farmers was gathered through a questionnaire-based survey that wa...

Muhammad Idrees1, Bashir Ahmad2, Muhammad Waqas1, Syed Muhammad Mukarram Shah3 and Saad Ahmad Khan4

...fied using computational methods. In the ligand base pharmacophore, an already reported four ligands for the four proteins encoded by the resistant genes of M. tuberculosis were selected for the generation of pharmacophores. The validated pharmacophores model of all the four proteins, generated on the basis of ligand base, were selected for further screening of ZINC drug like database. As a screening results 486 structurally diverse hits for katG, 542 for PncA...

Djadouni Fatima* and Meliani Amina

...erent ancient and modern methods of treating these diseases and to share them with other societies.

...

Ahmed M. Darwish1*, Hassan R. Darwish1, Dalia M. Mabrouk1, Mohamed A. Abdelhafez1, Ahmed M. Abdel-Salam2, Ibrahim E. Mohamed3, Ibrahim M. Farag1 

...etermined by biochemical methods in all milk samples. Different genotypes of β-LG and LEP genes were detected by single-strand conformation polymorphism (SSCP-PCR), and then validated by sequence analysis. The results of SSCP-PCR showed a monomorphic pattern for the β-LG gene and a polymorphic pattern for the LEP gene. The sequence analysis showed that the β-LG gene has one genotype without any single nucleotide polymorphism (SNP) while the LEP ...

Nahla Hamada Magd Khalil1*, Ihab Mahmoud Helal2, El-Desoky Hassan Ibrahim Dorrah1, Soad Ahmed Soliman Ismail3 

...y reduced in all cooking methods (100%). Heptachlor reduction by roasting was 86.36%, and completely reduced by microwaving and boiling. Heptachlor epoxide reduction was 94.88%, 79.49% after microwaving and roasting, respectively, and completely reduced by boiling. Concerning α-HCH, the reduction percentage was 95.83% for roasting, and 100% by microwaving and boiling while γ-HCH was completely reduced (100%) by all cooking ...

Aml M. Ragab1*, Maha R. Basyoni1, Enas A.I. Khoris2, Nadia A. Abd Elghany3 

... identified by molecular methods based on sequencing of genes, the potential of the groEL gene as a phylogenetic marker, and identified (hbl ,(nhe), (cytK), and (ces) enterotoxigenic genes. B. cereus isolates were analyzed for antibiotic susceptibility. A lab trial was conducted for two weeks using 60 Tilapia fish were divided into three equal groups, (1): kept as control negative, (2): infected intraperitoneally with (0.1ml) 8×107 (CFU/ ml/ fish) B. cer...

Muhammad Ali Raza1*, Aneela Zameer Durrani1, Muhammad Hassan Saleem1, Kamran Ashraf2, Muhammad Muddassir Ali3, Kumayl Hassan Akhtar4 and Nazia Rubab5

.... Kit detection and HPLC methods were used to identify ABs residues. The samples were collected from Lahore. Raw milk of buffalo and cow collected from the open market have shown 23.5% as the highest positive and UHT milk as the lowest positive 8.5%. Thus, it is an indispensable question to be further explored prospectively. 
 
Novelty Statement | The detection of antibiotic residues including peni...

Uzochukwu Ephraim Emeto, Chukwuemeka Calistus Okolo* and Nwakaego Ernestina Nweze

... dentition and normogram methods respectively. The results showed that 89.9% [confidence interval (CI) 86.4%, 93.4%] of the horses harboured gastrointestinal endoparasites including strongyles (81.3%), Parascaris equorum (8.2%), Oxyuris equi (21.1%), Strongyloides westeri (13.5%), Anoplocephala spp. (10.2%) Paranoplocephala mamillana (3.6%). Polyparasitism was prevalent (43.4%, CI 37.8%, 49.0%). Associations existing between age groups or gender, and the prese...

Jiang Wu*

...entified by conventional methods as Staphylococcus aureus, Salmonella, Escherichia coli and Listeria monocytogenes. Different bacteria were respectively inoculated into LB medium, the OD600 was adjusted to 0.5, and the initial bacterial concentration was adjusted to about 5×108 CFU/mL. T. officinale were dried in a hot air oven, and chemical substances in the dried powder were separated using Soxhlet extraction method with light petroleum as organic solv...

Nkiru E. Ekechukwu, Felicia N. Ekeh, Chinenye M. Ohanu, Hope C. Ezinwa and Ifeanyi Oscar N. Aguzie*

...icking as the collection methods. Overall 12 species were recorded, Orba had the lowest species diversity with 7 species while Obollo-afor and Ikpa recorded 10 and 9 species respectively. Dipteran and Coleoptera were the most diverse and abundant order observed with Musca domestica (Diptera: Muscidae) being the most abundant species encountered. Equitability index of evenness revealed that Calliphora vomitoria, Blattella orientalis, Monomorium minimum, Dermest...

Aiman Batool1, Muhammad Sohail Sajid1,2*, Hafiz Muhammad Rizwan3**, Asif Iqbal4, Imaad Rashid5, Ibadullah Jan6, Faiza Bano7, Fiaz Ahmad8, Waqas Ahmad1, Muhammad Nisar Khan1 

...itative and quantitative methods, and Giemsa-stained thin smear, respectively. Morphological identification of ectoparasites was carried out under the stereomicroscope. The overall prevalence of GI parasites, haemoparasites and ectoparasites in the small ruminant population was 74%, 16% and 53%, respectively. Sheep were found more prone to endoparasites conversely ectoparasites were more prevalent in the goat population. A higher prevalence of GI, haemo and ec...

Mimi Syazwani Jaapar1, Eric Lim Teik Chung1,2*, Nazri Nayan1, Mamat Hamidi Kamalludin1,2, Mookiah Saminathan3, Kalai Vaani Muniandy2, Muhammad Hazziq Mohd Hamdan1, Shokri Jusoh1, Faez Firdaus Abdullah Jesse2,4 

...according to established methods and data collected were subjected to one-way analysis of variance (ANOVA) and Duncan’s Multiple Range Test to determine the level of significance among treatments. The net gas production and gas production kinetics demonstrated notable changes (P < 0.05) among treatments. Besides, only the acetic acid and total VFA showed significant differences (P < 0.05) for the rumen fermentation characteristics. Generally, the T...

Junaid Ali1, Muhammad Nawaz2, Muhammad Ilyas3, Muhammad Umer Chattha1, Imran Khan1, Muhammad Bilal Chattha4*, Muhammad Arshad5, Muhammad Akram6, Mina Kharal7, Ehsan Ullah1, Muhammad Talha Aslam1, Fareeha Athar1, Ayesha Mustafa1 and Muhammad Umair Hassan1

...eral and protein. Sowing methods and irrigation application are an important agronomic consideration to get maximum production of lentil crop. Therefore, this study was conducted in RCBD with a two-factor split plot to determine the impact of various levels of irrigation and sowing methods on the growth and yield of lentil. Crop was sown by three sowing methods; flat, bed and ridge and wit...

Syeda Rubab Zaidi* and Safdar Ali Mirza

...ation and anion exchange methods were used along with protein estimation. The purified fraction was then subjected to enzymatic characterization to find its thermal stability, effect of pH and selected chemical compounds. Other significant experimental findings were; optimum incubation period was 7 days, inoculum size was 3 discs of 0.5 cm, CuSO4 as inducer improved production, laccase activity of crude extract i.e. 10.89U/ml increased to 15.07U/ml with purifi...

Saima Mehar1 and Salma Javed2*

...sed through spectroscopy methods such as Fourier-transform infrared spectroscopy (FTIR), Ultraviolet–visible spectroscopy (UV), Mass spectrometry (MS), and Nuclear Magnetic Resonance (NMR). The structure of isolated chemical components employing bioassay-directed fractionation of both extracts. Concentration of the extract was directly proportional to the mortality of second stage juveniles, the resulted, six withanolides comprising coagulin E, G and J, ...

Imtiaz Ahmed*, Imran Khan and Zia Ud Din

...performed using standard methods. Folin-Ciocalteu, Aluminium chloride, and Colorimetric assays (ABTS and DPPH) were followed to determine total phenolic content, flavonoid contents and antioxidant status respectively along with“in-vitro starch digestibility”. The ash, fiber and protein content increased significantly while the carbohydrate content had significantly lower values (p, for all trends < 0.05) as the ratio of incorporation increased w...

Abdullah Channo1*, Asmatullah Kaka1, Qudratullah Kalwar2, Imdadullah Jamali3, Ghulam Jelani2, Muhammad Bakhsh4, Ghulam Nabi Dahri5 and Jai Parkash Goil6

...s rectal ultrasonography methods have been used. COD should be treated by using different treatment protocols such as hormonal, medicinal and homeopathic medicines.

...

Veronica Ngozi Emenuga1, Clara Idara Eleazar2*, Seto Tunrayo Aladenika1  

...rried out using standard methods. Antifungal biogram was performed. Result showed that all the cattle and the bedding tested positive for fungi, while 41(82%) of the milk samples had fungal contamination. Rate of isolation from cattle, bedding and the milk was not significantly (P= 0.365) different. The distributions of various species within the bedding was significantly different (P= 0.001). Trichophyton mentagrophytes had the highest rate in the beddings (...

Tinda Afriani*, Zaituni Udin, James Hellyward, Endang Purwati, Adisti Rastosari, Dwiki Wahyudi 

...min gradient and swim-up methods in Pesisir bull. The semen samples from three bulls were collected and evaluated for macroscopic and microscopic quality parameters including the percentage of motility, plasma membrane integrity (PMI) and acrosome intact (AI). The result revealed that the average motility, PMI and AI of sperms bearing X- and Y-chromosome in the albumin gradient method was 58.33±10.32% and 60.83± 13.57%, 53.63±16.54% an...

Lyudmila Yakovlevna Rodionova*, Irina Valeryevna Sobol, Lyudmila Vladimirovna Donchenko, Artem Vasilevich Stepovoy  and Andrey Georgievich Koshchaev

... standardized and modern methods of physical and chemical analysis of plant materials were used. The biochemical parameters of pumpkins were studied: the content of dry substances, sugars, titratable acidity, the content of ascorbic acid and carotene. Data on the content and fractional composition of pectin substances in different parts of pumpkins are presented. The kinetics of changes in the content of pectin substances in pumpkins after harvesting and stora...

Zahin Anjum1*, Farhat Shehzad1, Shaista Ali1, Sumbla Yousaf2, Amina Rahat1 and Hamid Ullah Shah3

...fect of various grilling methods on PAH levels was evaluated. Mutton grilled with three grilling procedures, were analyzed for its PAHs content. A total of 120 samples were collected from local food vendor spots or general restaurants, and control samples of gas and electric grilled meat samples were also prepared and analyzed. Significant difference sp≤0.05 in PAHs levels were observed in different grilling procedures. Among the mutton samples, the highest...

Jie Zhang, Na Li, Tingting Guo, Dandan Hu, Xiaofeng XU and Lili Zhang*

...ect of different feeding methods on the rumen bacterial flora of Tan sheep, a Chinese indigenous breed, using 16S rDNA high-throughput analysis. The rumen fluid was collected from 9-month-old Tan sheep that were either house-fed or grazed. The results showed that the diversity of rumen flora in the grazing group was significantly higher than that in the house-raised group (P < 0.01). In the grazing group, the abundance of 96 genera was significantly higher ...

Alexander Tsybulsky1*, Eduard Kostetsky1, Anton Degtyarenko2, Michail Shchelkanov1,2,3 

...se laboratory tests with methods for assessing the expression of interferon (IFN) genes can expand the possibilities of diagnosing animal cancers and improve the accuracy of differential diagnosis of benign and malignant neoplasms, in particular of the mammary gland (MG). Methods. Surgical material obtained after unilateral mastectomy with simultaneous hysterovariectomy, as well as peripheral blood samples of cats with MG tu...

Heba S.S. Salem1, Hend M. Megahed2, Marwa M. Sarhan2, Maha M. El Alem3, Gehan N. Alagmy3* 

... via traditional culture methods and molecular confirmation using PCR. All tested S. aureus isolates were positive for virulence-associated genes (nuc, icaA, and Hlg). In an experimental trial, a total of 75 healthy rabbits were divided equally into five groups (n =15/each group). The first group served as a negative control, the second group was injected intra-articulately with S. aureus, did not receive any treatment, and served as a positive control group,...

Shomaila Sikandar1*, Imran Afzal1 and Sadaf Sarfraz2

...rly mycotoxins detection methods and making regularity bodies to contain the spread of mycotoxins. This review summarizes the occurrence and toxicity of five major types of mycotoxins associated with food and feed and their importance in human nutrition and animal health.
...

Fengbin Jin1, Xin Liu2 and Yanqiu Yue3*

...he efficacy of these two methods was compared between two groups. Besides, we compared the changes in the vital sign (including blood pressure and heart rate), blood-gas indicators and scores of symptoms before and after treatment between two groups. Additionally, we also observed the adverse reactions of patients in two groups. After treatment, the effectiveness rate of patients in the observation group and control group were 96.49% and 79.70%, and the differ...

Sohail Nadeem1, Muhammad Aziz2*, Ayesha Mohy ud Din1 and Tayyaba Jamil1

...de through computational methods as well as to evaluate its antifungal properties. In order to achieve the goal, softwares like Avogadro, Gamess, Gabedit, Chimera and free online databases were used. The infrared and raman spectra were computed at the harmonic level with restricted Hartree-Fock in basis sets 3-21G and 6-31G. Molecular docking is calculated using online server with Lanosterol 14α-demethylase to know antifungal properties. The theoreticall...

Huajun Lin, Wei Qu and Zhuxian Yu*

...reated with conventional methods. The clinical treatment effects, connective tissue growth factor (CTGF), TGF-β1 levels in serum and cognitive function were compared between the two groups. The total effective rate of treatment in the combination group was 93.75%, which was significantly higher than 62.50% in the control group (P<0.05); after treatment, the serum CTGF and TGF-β1 levels in both groups were higher than before treatment. The serum CT...

Indyah Aryani 1,3*, Suyadi Suyadi2, Hartutik Hartutik2, Kuswati Kuswati2 

...g whitlock sedimentation methods. The specific species was identified by their morphology. A total 43 Madura cattles were infected by nematode worm as follows: Oesophagostomum sp., Cooperia sp., Capilaria sp., Ostertagia sp, and Moniezia sp) while, 14 Madura cattles infected by trematode worms (Fasciola sp and Paramphistomum sp). The prevalence of gastrointestinal endoparasite was found as follows: Cooperia sp. 31%, Fasciola sp. 12%, Oesophagostomum sp. 6%, M...

Marcos E. Bollido1,2*, Renell Jay G. Villaluz1,2 and Ronald L. Orale1

...was examined using mixed methods. Supply and consumption were forecasted using Autoregressive Integrated Moving Average (ARIMA) models. Pork prices increased by 22-50% in 2021 compared to 2010 data. Live-weight pig prices also increased by 9-39% for the same year in review. It was noted that 27% of the supply was locally produced, and 73% were imported from other regions. Locally sourced pig supply is pegged at 53%, and the rest (47%) are imported from various...

Farheen Shaikh*, Saima Naz and Nadir Ali Birmani

...e collected hand picking methods with fine brushes and preserved in 70-75% ethyl alcohol. The permanent microscopic slides with cover slips were prepared for final mounting in Canada balsam. At present 130 specimens were recovered and classified into 4 species which belongs to 3 genera. The species and their prevalence are 19.23% for Menopon gallinae (Linnaeus, 1758), 23.84% for Menacanthus stramineus (Nitzsh, 1818), and 22. 30% for Menacanthus abdominalis (Pi...

Shakeel Ahmad1*, Humaira Wasila2, Juweria Abid3, Nazir Muhammad4 and Hazrat Usman5 

...ample. AOAC 15th edition methods were used to analyze moisture, ash, carbohydrates, fats and protein contents of milk products. Atomic absorption spectroscopy and flame photometry were used for mineral analysis. Total phenolic compounds were evaluated by the Folin-Ciocalteu method. The aluminum chloride colorimetric method was used for evaluating total flavonoids contents. Antioxidant activity was assessed by the DPPH method. The results showed that moisture w...

Muhammad Said1, Amjad Hussain Mirani1, Abdul Kabir*1,2, Muhammad Haris Raza Farhan3, Abdul Latif Bhutto1, Ghulam Shabbir Barham1, Khaleeq Ur Rahman Bhutto4, Muhammad Uzair1, Maaz Khan1 

...primarily on the complex methods of mastitis detection, as identification of the etiological agents are vital to preventing mastitis in dairy cows.

Keywords | Mastitis, Diagnosis, Bovine Mastitis, Immunoassays, Diagnostic Techniques, Chronic Mastitis 

...

Ishtiaq Ahmad1*, Muhammad Nafees1, Maryam2, Irfan Ashraf3, Ambreen Maqsood4 and Muhammad Saqib5 

...tion of different drying methods to improve shelf-life of dry mulberry fruits for their sustainable availability in the market for human consumption. For this purpose, common drying methods viz., sun drying, shade drying and oven drying were applied on freshly harvested black and white mulberry fruits to evaluate the significant drying method with minimal nutritional losses. The fruit samples were collected from mulberry pla...

Ansar Abbas1*, Muhammad Aman Ullah2, Abdul Waheed3 and Muhammad Asif4

...alli sheep by using both methods. However, the comparative analysis shows that the CHAID algorithm performed well as compared to the MLR (CHAID vs. MLR: R2 % = 79.38 vs. 73.27; Adj-R2 %= 78.70 vs. 72.50; SD ratio=0.45 vs. 0.51; RMSE= 3.15 vs. 3.58; RAE= 0.12 vs. 0.14; MAD= 2.41 vs. 2.74; CV%=13.28 vs. 15.11; AIC =638.50 vs. 708.28; r= 0.89 vs. 0.85). Consequently, it is hoped that the results of the study on the morphological characterization of Thalli sheep m...

Neni Widaningsih1,2*, Budi Hartono2, Hari Dwi Utami2, Eni Siti Rohaeni3 

...Kalimantan Province. The methods used in this study are survey and observation methods. Data collection carried out includes; purposive sampling and accidental sampling techniques with the main respondents, namely breeders, collecting merchants, wholesalers, retailers, and consumers. The number of respondents in this study was 135 persons. Methods of data analysis carried out include; qual...

Fahrauk Faramayuda*, Soraya Riyanti and Sitty Mahanadhiandinie

...donesia using extraction methods other than brewing, i.e., maceration. Study of free radical scavenging activity of 1,1- Diphenyl-2- Picrylhydrazyl (DPPH) and the determination of the total polyphenol content of white tea leaves have been conducted using UV-Visible spectrophotometry. Results of the study revealed that the antioxidant activities of the ethanol extract, n-hexane fraction, the water fraction and the ethyl acetate fraction of white tea leaves had ...

Shama Sadaf1*, Komal Hassan1, Ayesha Saeed1 and Zeeshan Ahmad2 

... using ISO standard test methods. The fabric which was treated with A. indica showed 100% reduction on all samples after 22 hours, but after six days only one colony of microorganism was appeared on treated fabric, while nine colonies was observed on untreated cotton sample The cotton fabric treated with B. monosperma and L. chinensis exhibited 100% reduction after 22 hours and even after six days interval. In case of mechanical properties (Tensile strength, T...

Ahmed M. El-Waziry1,2*, Saeid M. Basmaeil1, Ibrahim A. Alhidary1, Gamaleldin M. Suliman1,3, Mutassim M. Abdelrahman1, Maged A. Al-Garadi1 

...eed. Among the available methods of increasing production is using feed additives. One class of these additives is ionophores, a type of antibiotic that increases the nutritional value of the feed, thereby increasing production by reducing of feed intake, and decreasing the degradability of protein in the rumen, which increases its by-pass to the small intestine and decreasing the ammonia concentration in the rumen. Moreover, ionophores increase propionic acid...
Emel Hulya Yukseloglu1, Fatma Cavus Yonar1*, Omer Karatas1, Gulten Rayimoglu1, Faruk Asicioglu1, Elif Canpolat1, Onur Ozturk2 and Itir Erkan3

Eighty Mardiyan Kurniawati1*, Budi Santoso1, Fedik Abdul Rantam2, Widjiati3, Budi Iman Santoso4, Tri Hastono Setyo Hadi1, Gatut Hardianto1, Hari Paraton1  

Mochamad Rizal Umami1, Zainuri*2, Sebastiana Viphindrartin2 and Rafael Purtomo Somaji2

...yzed through descriptive methods and content analysis. The study found that the COVID-19 pandemic formed a new pattern of sugarcane trading system, switching from a contractual trade model to a free market model (redeemer). The model refutes the theory stating that the contractual model will be more efficient than the free market model due to its lower transaction costs. The proposition (substantive theory) produced in this study states that in conditions of s...
Alia Tajdar1,3, Shaghef Ejaz2, Muhammad Shah Zaib1, Anum Ishfaq1, Syed Muhammad Zaka1*, Hafiz Muhammad Safeer1 and Khalid Abbas1
...ter harvesting different methods are used (cultural practices, mechanical practices, biological practices), but chemical control practices are dominant among all. Besides quick and effective outcomes of chemical control. It also has side effects (residues in fruits, human health issues, resistance, damage to non-target population, environment pollution). In the present study the effect of different botanical extracts on the olfactory response of B. zonata and ...

Manzoor Ali Magsi1*, Naimatullah Laghari1, Ahmed Ali Tagar1, and Habibullah Magsi2

...e most efficient tillage methods for the wheat crop were shallow tillage + compost and deep tillage + NPK respectively. On the other hand, the highest calcium (Deep + FM), magnesium (Deep + Poultry, Shallow + FM), chloride (Deep + NPK and compost), and carbonate and bicarbonate (Deep + FM and Compost) treatments were found during the cropping year 2019-20 and 2020-21 experiments. Based on experimental results under wheat crop, application of reduced tillage tr...

Shahid Hameed Khan Khalil1*, Abdus Subhan3, Ghani Akbar1, Muhammad Asif1, Zafar Islam1, Adnan Shakeel1 and Fawad Anwar2 

... conventional irrigation methods, irrigation was used to maintain field capacity using 33kPa tension-meter reading during tillering, stem elongation, tillering and grain formation stages. It is clear from the experiment results that parameter of wheat growth including plant height, days to harvest, and number of tillers per plant and grain weight per ear were significantly changed by the application of different amounts of water (treatment) during the two year...
Huma Abbas1*, Nazir Javed1, Muhammad Kamran2, Sajid Aleem Khan1, Abdul Jabbar1, Akhtar Hameed1, Hira Abbas3, Azhar Iqbal2 and Ehetisham-ul-Haq2
...s. Different application methods regarding protective and curative were used to test the efficacy of Cartap, Virtako, Cure and Azadirachtin. Results revealed that Protective application of bio and synthetic chemicals was more effective at nematode suppression rather than being curative. Then the impact of bio and synthetic chemicals were tested in combination with NPK on nematode reproduction. Following treatments i.e. Virtako + NPK, Cartap + NPK, Cure + NPK, ...

Jin-Ming Zhao1,2, Yun Fang2 and Yue-Hua Sun2*

...racy compared with other methods, such as egg candling and floating. Our results confirmed the conveniency and accuracy of the weight loss method in estimating nest ages of Chinese grouse. We further discussed some cautions when applying the weight loss method. We recommended investigators to use the weight loss method rather than the candling or egg floating methods to determine avian nest ages in future field studies.

...

R. Hadadfar1, E. Mahdikhani-Moghadam2†, S. Baghaee2 and M. S. Bajestani1

...extracted by centrifugal methods. Psilenchus species were identified on morphological and morphometrical characters based on recent valid keys. Seven species from Psilenchus genus were identified viz., Psilenchus curcumerus, P. aestuarius, P. iranicus, P. hilarus, P. bilineatus, P. pratensis and P. bahiablancae of which P. bilineatus, P. pratensis and P. bahiablancae are new reports of these species for Iranian nematode fauna.

...

A. M. Rahoo1, 2, †, T. Mukhtar3, B. A. Bughio4, S. R. Gowen1 and R. K. Rahoo5

...d
extraction methods was investigated on the production of IJ in Galleria mellonella. There was significantly greater
emergence of IJ of S. affine from the Galleria mellonella using White traps than with modified Baermann extraction
trays. In Baermann extraction trays, the maximum emergence of IJ of S. affine was observed at an inoculation dose
of 400 IJ followed by the dose of 50 IJ....

M. A. Radwan†, M. M. Abu-Elamayem, S. A. A. Farrag and N. S. Ahmed

...
application methods significantly reduced tomato root galls and 2nd stage juvenile numbers in soil compared with
control. Except DNSA and AA, foliar application of tested organic acids was more effective in reducing nematode
galls than soil drench application. Foliar application of ASA caused superior effect in the reduction of J2 in soil (100
%) followed by AA (89.44 %), OA (88.25 %) and SA (70.69 %). CA (96.52...

Nadia A Eltablawy1, Ibrahim El Tantawy El Sayed2, Hamed Mohamed Abdel Barry2, Marwa A. Ibrahim3*, Maha Nageib Ahmed Serag ElDein2 

Istkhar† and A. K. Chaubey†
...
neighbour joining methods and comparative account on nucleotides analyses based on ITSs study confirmed the
present specimens as isolates of S. abbasi. The nucleotide composition resemblance within 5.8S gene with other
Steinernema species evident the less polymorphism and highly conserved nature of this region as compared to ITS1
and ITS2 and delimit the relationship of steinernematid nematodes.
...

A. Sattar1, †, A. Khan2, N. Khatoon1 and A. Mujahid3

...asitological
methods. The nematode parasites namely; Raphidascaris acus (Bloch, 1779) larvae, Metabronema magnum (Taylor,
1925), Haplonema immutatum (Ward et Magath, 1917) and Hedruris bryttosi (Yamaguti, 1935) were recorded from
the gut of the catfish, Arius arius (Hamilton, 1822). These are new host records as these parasites have not been
reported from Arius arius species in the region, off the Karachi coast.<...

F. Shahina† and G. Mehreen

... by using four different methods i.e.,
Maximum Evolution (ME), Maximum Likelihood (ML), Maximum Parsimony (MP) and Neighbor Joining (NJ)
based on the two makers. Sequence composition and phylogenetic analyses of these isolates showed closeness with
Steinernema  abbasi. On the basis of ITS rDNA region these seven Pakistani isolates were compared with seven
worldwide isolates of S. abbasi and species of bicorn...

F. Shahina† and G. Mehreen

...cted using two different methods maximum parsimony (MP) and
Bayesian inference (BI) in which most of them form highly to moderately supported clades.
...

Rinat Islamov*, Dinara Turegeldieva, Altyn Rysbekova, Kuralay Sarmantayeva, Victor Semenuyk 

... were selected. The main methods of testing biological samples from laboratory mice and rats were realtime polymerase chain reaction and enzyme-linked immunosorbent assay. A high prevalence of some pathogens among conventional laboratory animals used by some academic institutes and universities of Almaty has been shown. Rodentibacter pneumotropicus has been detected in the SPF animals, while no other pathogens from the FELASA list have been detected. The obtai...

Ahmad Abrar Khan1*, Ghulam Qadar1, Asif Ali Abro2 and Muhammad Awais3

...n of right time, control methods, appropriate insecticides/pesticides along-with recommended dose and treatments must be defined by the recommendations of agriculture experts instead of fellow farmers and agrovet sellers. Trainings, workshops and field activities should be organized regarding improved farm management practices especially on the aspect of insects/pests management to minimize the yield losses. 

...
Aulia Evi Susanti1*, Rudy Priyanto2, Muladno2, Dewi Apri Astuti3, Lucia Cyrilla2
...lected through interview methods to obtain data on production systems and business models, reproductive and economic parameters, and feed applications to obtain production parameters at the Field Station-School for Smallholder Community (FS-SSC) Maju Bersama, Musi Banyuasin Regency in 2021. The feed application experimental design used a factorial 3x2 Randomized Block Design (RBD) with 10 replications. A descriptive analysis was conducted to evaluate the corpo...

Sutawi*, Ahmad Wahyudi, Abdul Malik, Suyatno, Asmah Hidayati, Imbang D. Rahayu, Endang S. Hartatie

...condary data exploration methods. There are three factors that can be attributed to the re-emergence of FMD outbreaks in Indonesia. First, the loss of the principle of maximum security. Indonesia’s success in freeing itself from FMD after 100 years is thanks to the implementation of maximum security policy by enacting a country-based system, which is to only import livestock and livestock products into the country from FMD-free countries. Second, the sha...

Syed Shah Mohioudin Gillani1, Muhammad Naveed Tahir1, Adeel Anwar1*, Syed Ijaz Ul Haq2, Muhammad Awais3, Mujahid Iqbal4, Javed Iqbal5, Hina Ahmed Malik3, Syed Muhammad Zaigham Abbas Naqvi6, Raza Ullah7 and Muhammad Abdullah Khan1

... through non-destructive methods enable us to understand the different environmental impacts and help to respond to them precisely and rapidly. Chlorophyll pigmentation in plants is a supreme factor to assess the health of plants and ultimately leads to crop yield estimation. The present experiment evaluated the capacity of high-resolution satellite imagery to estimate wheat chlorophyll content coupled with ground-based information for accurately monitoring cr...

Saba Iqbal1, Salman Khurshid1*, Hafiza Mehwish Iqbal1, Qurrat-Ul-Ain Akbar1, Aqeel Ahmed Siddique3, Saqib Arif1, Shahid Yousaf2, Masooma Munir4, Abdul Karim Khan4, Shazia Arif5,  Abdul Ahad6 and Muhammad Arif7

...nalyzed through standard methods for hardness such as hardness index (HI) and particle size index (PSI). Samples had hardness scores ranging between 55.5 and 79.1. A positive correlation shown among the values of NIRS and HI (r = 0.92). However, PSI values were weakly related to HI (r = -0.46) and NIR (r = -0.30) values. The grouping of data further suggested that the samples belonged to the medium and hard wheat classes. The correlation coefficients (r) evalu...

M.M. Mikailov1*, O. Yu. Chernykh2, E.A. Yanikova1, G.A. Nurlygayanova3, O.D. Sklyarov4 

...f most other serological methods. Data from comparative serological studies of cattle blood serum in RNGA, ELISA, RA, RSK, RBP and RID with O-PS, confirmed the specificity of the tests and high sensitivity of RNGA with brucellosis erythrocyte antigen 

...

Dalia Zaafar1*, Heba M.A Khalil2, Soha Hassanin3, Mohamed R. Mousa4, Mona G. Khalil1 

Ghulam Jelani1, Muhammad Haris Raza Farhan2, Rida Asrar3, Muhammad Ahmad1,4*, Chanda Naseem5, Zafar Ali Khoso6, Majid Hussain Soomro7* 

...isolation and diagnostic methods as well as improper use of antimicrobial therapy causes the treatment failure. Different types of antibiotics are used to treat it and different research use antibiotics of aminoglycoside group. For preventing the flock from infection separates the infected birds from healthy ones. Different treatments show average growth and good performance with the recovery of clinical signs, histopathological changes, mortality, and morbidi...
 
 USMAN AHMAD*1, AMTUL JAMIL SAMI1, MADEEHA KARAMAT1, MADEEHA KHALID1, FAIZAN NAEEM2 & MUHAMMAD JAMIL YOUSAF
...ltrasonic and electrical methods. Drug delivery through the largest organ (skin) by dermal patches of psyllium based hydrogels was observed. In this article we have fabricated different psyllium based dermal patches of hydrogels for insulin delivery. Psyllium, a natural polysaccharide, is a dietary fiber and widely utilized in gel forming. To study the structural aspects of these various polymeric webs were categorized with FTIR, SEM and release related experi...

Irene S. Gamil1* and Dalia Fouad1,2

...rnorate, Egypt. Standard methods of collection of the snakes and examination of the cestode tapeworms for taxonomic studies were used. Ophiotaenia tessellata sp. n. was identified and being separable from Ophiotaenia species found in African snakes as well as those from colubrid snakes based on many morphological characteristics. Analysis of a dataset based on 473 bp of its 18S rRNA gene regions was carried out to determine the phylogenetic position of the new...
Nazish Huma Khan1*, Muhammad Sufyan Khan1, Tooba Saeed2, Mohammad Ilyas3 and Hazrat Husaain4
...medical and conventional methods need to be adopted depending on the severity of viral attack. Moreover, vaccination is also referred to improve the immunity against this viral attack.
...

Theresia Nur Indah Koni*, Tri Anggarini Yuniwati Foenay, Stormy Vertygo

...nt of 14-16%. One of the methods to reduce crude fiber is through anaerobic fermentation, which requires carbon from soluble carbohydrates such as palmyra sap and nitrogen from Non-Protein Nitrogen such as urea. This experiment aimed to evaluate the fermentation characteristics and nutrient content of rice bran fermented anaerobically for six days. This experiment used a Completely Randomized Design with four treatments and four replications. The treatments in...

Faradji Khalil1*, Slimani Noureddine2 and Senoussi Abdelhakim3

...te observation or survey methods and the use of analysis means adapted to the situations encountered. In this context, the means used to carry out this work are based on documentary research, surveys and observations. Based on field investigations, three main categories of livestock breeders were distinguished the farmers surveyed have at least an education level thanks to the reforms carried out by the Algerian state in the field of rural development and that...

Ijaz Haider1,2*, Muhammad Riaz2, Sikandar Ali2, Qurban Ali1, Ali Noman3, Dilbar Hussain1, Imran Nadeem1, Muhammad Faheem Akhtar1, Aqsa Abbas1, Asad Aslam1, Hafiz Saad Bin Mustafa2, Ejaz Ul Hassan2, Muhammad Zubair2, Muhammad Saleem1 and Muhammad Kamil Malik1

...cluding chemical control methods. Due to certain environmental issues and insecticide resistance problems, adoption of alternate methods of insect control is imperative. In this experiment we used some insecticides alone and in combination with Salicylic acid against B. tabaci. Flonicamid 50% DF at the rate of 80 g/ac and Diafenthiuran 50% SC at the rate of 200 mL/ac were used against Whitefly. Salicylic acid (SA) by Sigma-A...

Shaimaa M. Ahmed1, Gehan M.A. Kassem1*, Fernando Pérez-Rodríguez2, Heba H.S. Abdel-Naeem1 

...urbidity and plate count methods as well as monitoring and modelling its potential growth in chicken breast and thigh meats at room temperature (25 °C) and refrigeration temperature (4 °C). The calibration curve showed a linear relationship between the turbidity measurement and bacterial cell count in TSB. Moreover, the coefficient of determination index (R2) was 0.8789 which indicates that the linear function was acceptable for reflecting the relation...

Mostafa Mohamed Attia Hammam, Moawad Mohamed Mohamed Mohamed* and Mahfouz Mohamed Mostafa Abd-Elgawad

...esents effective control methods as Egypt is the world’s largest importer of faba bean. Using gain thresholds, the study compares costs of a biological versus a chemical nematicide for M. arenaria control on faba bean. Meloidogyne arenaria was not detected at pre-planting soil samples of two consecutive field seasons. However, the potential of field weeds as hosts/reservoirs for M. arenaria to transfer from weeds and build-up on ‘Giza 843’ fa...

E. K. Allam.1; B. A. Othman.1; Hayam S. Abdelkader2; and Noher A. Mahmoud2

... and molecular detection methods provide tools assisting in the understanding of the epidemiology and diversity of nepoviruses as well as to facilitate resistance breeding, cultivar selection, and development of control strategies.

...

M.A.S. El-Kady1, Om-Hashem M. El-Banna 2, E.A Salama2, Salwa N. Zein1

...n of BSMV by serological methods i.e., Enzyme-linked immunosorbent assay (ELISA), Dot-blot and tissues-blot immunobinding assays (DBIA and TBIA). The presence of the virus was confirmed in mature seed parts and non-seed parts of the different five barley cultivars tested.

...

Waheed Khan1, Muhammad Ajmal Khan2, Bakhtawar Khan1*, Aftab Amin3, Muhsin Ali4, Awais Farid, Ali Hazrat5 and Muhammad Yahya5

...ded to investigate novel methods to improve DM treatment. Thus plant-based management could be a possible antidiabetic strategy. The objective of the most recent study was to assess Verbascum thapsus’s ability to combat hyperlipidemia and diabetes. Mice were given an intraperitoneal injection of alloxan (150 mg kg-1, b.w.) to cause diabetes. There were five groups of mice (n=10): group 1 (normal control) received normal food and water, group 2 (diabetic ...

Muhammad Ismail1, Abu Bakar Muhammad Raza1,2, Zarina Qasim3, Muhammad Zeeshan Majeed1*

...l available pest control methods with minimum implication of hazardous synthetic insecticides. In this study, five treatments including field sanitation (T1), pheromone-based attract and kill strategy (T2), protein-based bait method (T3), combination of all three methods (T4) and control (T5) were evaluated against B. dorsalis infestation in citrus during 2015 and 2016. Data of percent infested fallen fruits, percent pupae r...

Behari Lal Meghwar1*, Attaullah Khan1, Imran Ali Lakhiar1, Asif Ali Mirani2, Muhammad Siddique Daper1 and Muhammad Waseem Kalroo

...red two different drying methods, solar tunnel (T1) and solar cum gas (T2), and compared the rate of reducing the moisture over time with T3 method. The T1 and T2 methods are substitutes for the T3 drying method. Therefore, a small-scale natural convection solar tunnel dryer and solar cum gas-based drying methods were developed and tested at PARC-Arid Zone Research Center, Umerkot. This st...

Pengliang Xie1, Lufang Zheng2*, Mingxia Dong3, Huaiqiang Zhang1 and Fang Chen1

...nd develop new treatment methods.

...

Shaimaa A.Tawfik2,3, EL S.T. Awad1*, Hoda O.Abu Bakr1, Amira M.Gamal-Eldeen4, Esmat Ashour2, Ismail M.Ahmed1  

Luqman1*, Zahid Hussain2, Tamana Bakht3, Fazli Wahab1, Miftah-ud-Din1, Haroon Khan2, Imran Shinwari1, Ata Ullah1, Liaqat Khan1 and Sara Hidayat

Yue-Xia Ding1, Qun Wu2, Zhao Namula1 and Yi Ma1,*

...drug resistance transfer methods was lower than that of their parents.

...

Khitam J. Yahya*, Mohammed T. S. Al-Zubaidi 

...ium spp. using molecular methods (PCR), followed by DNA sequencing. The overall infection rate was 37% (37/100) of the samples were Cryptosporidium-positive by using PCR. Three of five quails whose 18S gene was amplified by nested PCR and sequenced were found to belong to C. meleagridis in the sequence of this gene deposit on the website of the National Center for Biotechnology Information (NCBI) under accession number (HQ917077.1), while the others were found...

Nawfal Hammadi Jasim1, Yasmeen Jassim Mohammed2 , Jala Amir Salman Alahmed1, Majdy Faisal Majeed3* 

...his study. Materials and methods: receiving an oral dose (2 daily MPH doses), represented by Group 1 (5 mg/kg/day in a 5% glucose solution), Group 2 received daily dosages of methylphenidate in escalating amounts (5, 8, 12, and 16 mg/kg/day in a 5% of sucrose solution), as well as Group 3 as control animals ( 5% glucose solution). Group 1 resembled the therapeutic doses administered to humans, while Group 2 simulated the wrong use of some of the human addicts...

Jawad Ali1,*, Samiya Rehman1, Aqsa Aslam1, Fouzia Tanvir2, Rida Liaqat1, Muhammad Ahmad1, Aamir Riaz1, Muhammad Shafique3, Hassan Ali1 and Amjad Ali1

...PH and Phosphomolybdenum methods. Results shows leaves extract from Mianwali has the highest total antioxidant capacity while stems collected form the Sargodha has highest free radicals scavenging ability with values of 47.60± 3.00 Vit C equiv mg/g DW and 50.00± 3.00 IC50 value. 

...

Tasew Getu1*, Wassu Mohammed2, Awol Seid3, Tesfamariam Mekete4 and Bekele Kassa

...the effect of irrigation methods, cultivars, vermicompost (VC) and their interactions for management of the MI and RS disease complex under field conditions. The experiment was laid out in a split-split plot design with three replications. The findings indicated that the treatments and their interactions significantly (P ≤ 0.05) affected the development of MI, RS, and impacted potato yield. The cultivar Gudenie had the lowest mean value (2.2) of MI reproduc...

Salvator Minani1,2*, Eric Nsengiyumva1, Anatole Bigirimana1,2, Arnaud Cubahiro1,2, Dieudonné Ntakirutimana1,2 and Vénuste Bizoza1,2

...rundi. Effective control methods of liver flukes for intermediate and definitive hosts should be implemented to control fascioliasis in Burundi.

...

Antonius Agung Wiono1, Maria Elizabeth Nana Wijayanti1, Dhika Yonika Primacitra1, Trioso Purnawarman2, Andriyanto3, Aulia Andi Mustika3, Amaq Fadholly3* 

Luigi Liotta1, Vincenzo Lopreiato1, Fariborz Asroosh2 and Alireza Seidavi2*

...ccording to the standard methods. Macro and micro elements of milk were analyzed using inductively coupled plasma mass spectrometry. Data were subjected to the two-way ANOVA analysis to determine the significant differences between species/breeds. The acidity, density and fat in Talesh sheep’s milk was higher than milk from other species/breeds (P<0.01). Also, the amount of protein in Talesh sheep’s milk was significantly higher (P<0.05) than...
Muhammad Rizwan1, Hamid Akbar1*, Aftab Ahmad Anjum1, Muhammad Arif Khan1, Aneela Zameer Durrani2, Muhammad Abid Hayat3, Anjum Masood4, Muhammad Talha Sajjad1 and Nadeem Raza2
...y authenticated standard methods. We observed that serum levels of MDA and BHBA were higher at day 0 to day 14 (P<0.01), while the level of nonesterified fatty acid (NEFA) was higher at day 0 to day 7 (P<0.01) which dropped down to normal at day 21 (P>0.05) post-surgery in Group-B than Group-A. Moreover, CAT level in serum decreased (P<0.01) at day 0 to day 7, then it gradually increased to normal on day 14 to day 21 (P>0.05) post-surgery in Gro...

Mehwish Malik1*, Zanib Sadia2, Muhammad Sajid3, Hammidullah1, Yasir Amin1, Zubair Ali1 and Sohail Ahmad1

... microbe’s culture methods and biochemical tests. The E. coli isolated is further tested for ESBL E. coli. The detection of ESBL E. coli is done through DDST and CDT. It was noted that out of 210 samples 08 samples were found negative (no growth of any bacteria found) and 20 samples were shown growth other than E. coli suggesting a total prevalence of E. coli as 86.6 %. The prevalence of ESBLs in cecum of broilers in the present study is 53% of the total...

Isatay Tusupovich Jakupov, Gulzhan Tursunovna Yeszhanova, Gulnur Kurbanalievna Mamytbekova* 

...ere examined by clinical methods after calving, of which 45 cows showed signs of acute endometritis. Subsequently, the bacterial background of uterine mucus in sick cows was investigated and St. pyogenes, E. faecalis, and E. coli were isolated. For the complex treatment of acute postpartum endometritis, the cows of the experimental groups were treated with the phytopreparation C. leucocladum. For comparison, the drug «Biometrosanit» was used in the...

Alisher Safarov1*, Nasreen Nasreen4, Firuza Akramova5, Shukhrat Djabbarov1, Adolat Mirzaeva5, Javokhir Esonboev5, Djalaliddin Azimov5, Mourad Ben Said2,3 

Luthfiralda Sjahfirdi1*, Zulfi Atsiil Suandhy1, Tatang Mitra Setia2 

... all-occurrence sampling methods. Nest-building skills were assessed using the Likert scale. Moni (male orangutan) was always observed to nest using used nests. Meanwhile, Terra (female orangutan) was frequently observed building new nests, although she was caught sleeping on the forest floor several times. During observations, it was found that they prefer nest trees with a circumference of more than 1 m with a reasonably dense canopy refers preferred trees t...

Nadia Jabeen1, Abdul Mubeen Lodhi1*, Rehana Naz Syed1, Muhammad Ali Khanzada2 and Alia Gul3

...d by sun and oven drying methods. The area of District Mansehra is enriched with natural vegetation and can be explored for new species.

...

Asim Zubair1*, Anam Farid Chishtti2, Zohaib Atta Mehdie3, Aamna Arshad3, Ghulam Akbar Malik4 and Iffat Batool5

...tified using statistical methods. The findings show that women dominated practically all farming operations in the chosen area.

...

Amthal Ahmed Fouad1, Basem Mohamed Ahmed2, Momtaz Abdelhady Shahein1, Hussein Aly Hussein2* 

Deny Juniwati1, Hadri Latif2*, Trioso Purnawarman2, Zhang Shuqi3

 

...n EBN from two different methods. Nitrite levels in the samples were measured using a UV-Vis spectrophotometer. Utilizing an ultrasonic wave frequency of 40 kHz for 20 seconds could reduce nearly 80% of nitrite levels. It was considered more effective than standard methods, which were only able to reduce nitrite levels by up to 60%. 
 
Keywords | Edible bird nest, Nitrite, Ultrasonic wave

Saddam Hussein Mahmoud* and Shaimaa Nabhan Yassein

...ed by using conventional methods such as the germ tube test, urease production, Chlamydospore formation, and chromogenic medium. Hemolysis, phospholipase activities, and biofilm formation were among the virulence tested factors. Hemolysis and phospholipase activities were detected in 76.2% and 83.33% of C. albicans isolates, respectively, while biofilm formation was detected in 100% of C. albicans isolates. The diagnosis was confirmed using 25 of the most viru...

Bing Yan1, Meng Meng Zhang2, Xi Chen2, Yongming Li2*, Muhammad Khan3* and Tonghui Ma1*

...t to improve therapeutic methods, the prognosis of GBM still remains very poor. The poor prognosis is associated with cancer stem cells (CSCs) which hold the potential of self-renewal, and differentiation into pathogenomic heterogeneous tumor formation leading to therapeutic resistance. Therefore, targeting CSCs and their associated signaling pathways is crucial for effective treatment of GBM. In this study, we showed that alantolactone (ALT) hold the capacity...

Feng-Yan Liu1,2,3, Ting Chen1,2,3, Yu-Hui Li1,2,3, Zheng Liu1,2,3, Jing-Jin Zheng1,2,3, You-Bang Li1,2,3* and Zhong-hao Huang1,2,3*

...study, with non-invasive methods, we measured physiological hormones including glucocorticoids (GCs), cortisol, and thyroid hormones (THs, T3 and T4) of rhesus macaques’ (Macaca mulatta) fecal pellet sampled from the Longhushan Nature Reserve, Southwest Guangxi, China. The results indicated that cortisol was negatively correlated with ambient temperature and sunlight, whereas T4 was positively correlated with sunlight. Cortisol levels sampled from spring...

A.M. Abdul Azeem1, A.N. El shahat1* and Mohamed H.M. Abd el Megid2

...to physical and chemical methods because it is inexpensive and considered environmentally friendly. Therefore, the objective of this study was to synthesize selenium nanoparticles from Citrus paradise peels extract (SeNPs-CP), their general characterization, and their assessment for antioxidant activities against liver injury induced by bisphenol A (BPA) in male rats. In this work, Citrus paradise peels extract used for reduction of sodium selenite (Na2SeO3) t...

A.M. Abdul Azeem1, A.N. El shahat1* and Mohamed H.M. Abd el Megid2

...to physical and chemical methods because it is inexpensive and considered environmentally friendly. Therefore, the objective of this study was to synthesize selenium nanoparticles from Citrus paradise peels extract (SeNPs-CP), their general characterization, and their assessment for antioxidant activities against liver injury induced by bisphenol A (BPA) in male rats. In this work, Citrus paradise peels extract used for reduction of sodium selenite (Na2SeO3) t...

Wei Wang1, Meifeng Zhou2, Yuebo Liang1, Fan Zhang1, Zhong Wu3, Shaowei Mo4* and Yi Qing Wang1*

...the clinical therapeutic methods of HER2 positive breast cancer.

...

Stepanus Pakage*, Daud Krey, Trisiwi. Wahyu Widayati, Deny Anjelus Iyai, Agustinus Gatot Murwanto, Alnita Baaka, Daniel Yohanes Seseray, Djonly Woran 

...elihood Estimation (MLE) methods on the Cobb-Douglas production function model and the Technical Efficiency Effect Model option. The results showed that piglets, feed, and firewood had an effect on pig production. Pig farmers in Manokwari Regency have been allocatively efficient, but technically and economically inefficient. 

...

Arnold Christian Tabun*, Ferdinan Suharjono Suek, Cardial L Leo Penu, Johanis A. Jermias, Thomas Lapenangga  

...rome b (CYT B) gene. The methods used CYT B gene base methods of DNA analysis such as DNA extraction, Polymerase Chain Restriction, DNA sequencing, and identification of DNA sequences nucleotide. Bali cows with sorrel, black and white coat colors are used with an age range of 3-5 years. DNA sequencing conducted multiple alignments, the identification of the genetic distance, and the preparation of phylogenetic trees using th...

Nadeem Akmal1, Abid Hussain1*, Muhammad Yousaf2, Waqar Akhtar1 and Hassnain Shah1

...conventional rice sowing methods in the rice-wheat cropping zone of the Punjab province has been made by applying economic analysis technique. As, Basmati-Super and Basmati- 386 are main rice varieties grown in the country. Thus, this study is based on data of 46 purposively selected farmers for the crop season 2021, including 30 and 16 farmers of Basmati-386 and Basmati-Super, respectively. Cost comparison of the crop produced through mechanical and conventio...

Bdour Muhammed Al-Shweily1*, Jawad Bulbul Al-Zaidawi2, Muhammed Jubair Hanawi1 

...aken to identify optimal methods for its control. Due to the adverse effects of chemical pesticides on consumer health, particularly human health, the pursuit of alternative safe measures has been paramount. Among the prominent alternatives, biological control has garnered attention, wherein environmentally safe organisms such as nematodes and fungi are employed. This study examines their efficacy in controlling the insect larvae and pupae. The integration of ...

Muneer Abbas1*, Sohail Abbas2, Imran Faraz3, Niaz Hussain1, Muhammad Aslam1, Muhammad Irshad1, Mudassar Khaliq1, Abdul Ghaffar1, Zubeda Parveen1, Muhammad Nadeem1, Sana Ullah4* and Malik Akhtar Iqbal5

...: justify;">Conventional methods of insect control have faced significant challenges, primarily due to the extensive use of pesticides. This overreliance on pesticides has led to issues such as pest resistance and environmental pollution. Some formerly minor pests have transformed into major threats by altering their biology and host preferences. Integrated Pest Management (IPM) offers a promising solution by reducing the reliance on insecticides. Many countri...

Rimsha Ijaz, Rida Zahra, Naseer Ali Shah*, Haroon Ahmed, Nazish Bostan and Sadia Sattar

...having precise detection methods that allow for early diagnosis and care for better outcomes is critical.

...

Wei Fang1,2,3, Jiawei Hong1,2,3,4, Zhengyi Fu1,2,3, Jing Hu1,2,3, Gang Yu1,2,3, Zhenhua Ma1,2,3* and Humin Zong5*

...gene, and bioinformatics methods were then adopted to perform functional annotation classification, metabolic pathways and SSR feature analysis. The results showed that a total of 42.40 Gb data were obtained from transcriptome sequencing, and 38,817 Unigenes were obtained after assembly and de-redundancy; Using BLAST to annotate the assembled unigenes with seven functional databases (NR, NT, GO, COG, KEGG, Swissprot and Interpro), a total of 23,417 unigenes we...
Muhammad Abdul Mannan1,2, Sharmin Chowdhury2, Md Abul Hashem3,4 and Md. Hazzaz Bin Kabir1,5*
...ological and microscopic methods. The Polymerase Chain Reaction (PCR) was then employed to amplify the sec internal transcribed spacer (ITS-2) of the nuclear ribosomal DNA and the mitochondrial nicotinamide dehydrogenase subunit 4 gene (nad4) to identify H. contortus, followed by direct sequencing. Out of the 400 samples tested, 186 (46.5%) were positive for H. contortus. The parasite was found in 51.3% of sheep and 43.6% of goats. Through the sequencing analy...

Ihab G. AL-Shemmari1*, Ali Hussein Fadhil1, Mohammed Assad S. Alkabi1, Eman Jawad Jabber2 

SHEHLA AKBAR*, SAIQA ISHTIAQ*, KHALID HUSSAIN, ANS MUNIR & SAIRA REHMAN

...iv>
using standard methods for estimating the primary and secondary
metabolites. The maximum value of total carbohydrates (11.04μg/ml),
total starch (14.69μg/ml), total protein (14.34μg/ml), total amino
acids(28.43μg/ml), total lipids (3.67 μg/ml), total glycosaponins (14.95%),
total alkaloids (24.67%), total polyphenolics (28.40%) and total flavonoids
(5.67%) were found in given ...

Salam N. Aritonang*, Elly Roza, Rizqan, Winda Habsani 

...ths of age. The research methods used census and survey methods with purposive sampling techniques for determining the research location. We observed variables such as body weight, chest circumference, body length, and shoulder height. Data analysis was carried out descriptively based on arithmetic mean, standard deviation, and coefficient of variance. The result showed that the body weights of male and female buffalo crosse...

Orooba Mohammed Saeed Ibrahim1*, Rawaa Saladdin Jumaa2, Nibras Zeyad Yahya1 

...ion, agar well diffusion methods and time kill curve. Results revealed that the used juice extracts were variously bacteriostatic and bactericidal against the examined bacteria depending on concentration. Phytochemicals analysis indicated the presence of alkaloids, phenol, saponins, flavonoids, tannins, and glycosides. The test organism was more sensitive to C. limonum compared to C. aurantium. The MICs ranged from 1.56 to 12.5mg/ml; MBCs, (3.125 - 25mg/ml) an...
Fariha Javaid1*, Zahoor Qadir Samra1, Madeeha Shahzad Lodhi1,2, Aroosha Hussain1 and Gulnaz Pervaiz1
...t-effective purification methods. This research work aims to purify BRM by using magnetic nanoparticles. After purification, BRM enzyme was characterized and utilized in the detergent industry due to its stability at a wide range of pH and temperature; results showed that stem BRM has the highest enzyme activity at 23.5 U/ml as compared to crown leaf (11.7 U/ml) and peel (19.5 U/ml). The specific activity of leaf, peel, and stem BRM was determined 0.8, 1.2, an...

Putu Henrywaesa Sudipa1*, Hapsari Mahatmi1, Ketut Tono Pasek Gelgel1, I Gusti Ketut Suarjana1, I Nengah Kerta Besung1, Romy Muhammad Dary Mufa2 , I Wayan Sukernayasa3 

...i by using morphological methods. The samples were vaginal swabs obtained from 22 healthy female Bali cattle from various locations in Bali, Indonesia. For isolation, vaginal swabs were cultured on Sabouraud’s dextrose agar (SDA) medium and identification was performed macroscopically by observing colony growth and microscopically using the tape smear method stained with Methylene Blue stain. The results showed that 32 fungi belonging to seven genera wer...

Imtiaz Khan1*, Abdullah1, Muhammad Ibrahim1, Muhammad Ishfaq Khan1, Saima Hashim1, Shomayela Afzal2 and Khalid Nawab3 

...appropriate weed control methods to enhance crop productivity and reduce weed competition in chickpea cultivation. However, it is essential to consider factors like herbicide resistance, environmental impacts, and sustainability while formulating weed management strategies in agricultural practices. Further research may be needed to optimize the application of different treatments and to explore additional weed management techniques to maintain effective weed ...

Muhammad Nauman Arif1, Muhammad Mansha1* and Tanveer Hussain2

...y using in-house organic methods. PCR was used to amplify a total of 880 bp of overlapping genes, which were then sequenced. Bioedit software was used to align, and edit the sequences, and single nucleotide polymorphisms (SNPs) were evaluated. The MEGA X-based maximum likelihood method was employed to study the genetic relatedness between sequenced samples and reference sequence as well as other deer. Among query sequences, the greatest genetic distance was ob...

Sabi Ur Rehman1*, Laiba Arshad1, Saman Ali1, Shazma Massey2, Safeer Khan3, Abdul Samad4 and Fazal-Ur-Rehman5

...cance of green synthesis methods in nanoparticle production and their potential roles in various fields, including medicine and biotechnology.

...

Muhammad Mansoor1, Shahid Hameed Khalil2,4*, Muhammad Arshad Khan3, Ghani Akbar2, Muhammad Shahzad Khan4, Rana Naveed Mustafa2 and Salah ud Din5

...act of varied irrigation methods on mung bean growth, yield, and nodulation. Results showed superior performance in raised beds compared to flatbed systems, indicating their positive influence on mung bean outcomes. The study highlighted that a three-day irrigation schedule positively affected growth and nodulation, while a six-day interval was optimal for maximizing seed yield. Balancing vegetative growth and seed yield is crucial for overall productivity in ...

Merita Ayu Indrianti1,2*, Didi Rukmana3, Eymal Bahsar Demmallino3, Muh. Hatta Jamil3

...esearch used qualitative methods with a literature study approach supported by data from the Head of the Gorontalo District Agriculture Office as key informants. The results of this study indicate that the Gorontalo regency government currently has a regional regulation (PERDA) No. 2 of 2017 which regulates LP2B protection program, however, there are no clear geographical boundaries regarding the LP2B program in Gorontalo Regency also not implemented yet the r...

Dan Song1,2, Ming-Juan Ge3, Jie Li1,2, Yi Jiang1, Xiu-Mei Kong1, Jiao-Jiao Xu1, Xu Ji1,2, Rui-Xin Shi1 and Qin Zhao1,2*

...l extract. Three extract methods were employed to evaluate the best extraction methods. Male Kunming specific pathogen-free (SPF) mice were randomly divided into blank control, model (hypoxia), positive (propranolol 30 mg/kg + hypoxia), and three G. straminea ethanol extracts dose groups (10, 5 and 2.5 g/kg respectively + hypoxia), administered intragastrically once a day for 14 consecutive days. After that, multiple hypoxia...
Mahmoud M. Bayoumi
...production, the delivery methods, and the essential compositions added to the mRNA vaccines to enhance efficacy and stability. This information ultimately would pave the way to better thinking to combat viral infectious diseases.
...
Moustafa A. Zaghloul1*, Mohamed F. Azooz1, Saleh E. Ali1*, Heba M. Soliman1, Maha M. Sayed1, Mohamed H. Kafafy2 and Alaa R. Morsy1
...V. Several bioinformatic methods were employed to find superior epitopes. Fifty-three epitopes passed the three B-cell prediction tools. Twelve promising and top linear B epitopes passed the antigenicity, allergenicity and toxicity tests. Fifty-seven MHC I epitopes were the most promising, while (49) were from MHC II. Thirteen peptides for MHC II were chosen as the best targets for the vaccine. Seventeen MHC I epitopes were the most promising because they were...
Amany M. Reyad1*, Aya Maher Rabie1, Reda Mohamed Taha1 and Khalid El-Dougdoug2
...ntages over conventional methods of controlling pathogenic bacteria in which they have a high ability to self-reproduce, host specificity, and develop with their bacterial hosts. The most common pathogenic bacteria were isolated from fresh meat and identified using the VITEK II automated system. Phages specific to Escherichia coli, Staphylococcu...

Zhimin Wu1,2, Yaping Yang1, Arshad Zahoor1, Aftab Shaukat1 and Ganzhen Deng1,2*

...ut different measurement methods and indexes, the simple radiographic findings are difficult to be used in accurate clinical evaluation. This test aimed to develop a radiographic method of comparing canine posterior lung field angles include vertebrophrenic angle (cranial), vertebrophrenic angle (caudal), sterno-diaphragmatic angle, costophrenic angle (left), costophrenic angle (right) to identify whether pulmonary disordered. Meanwhile this method also introd...

Lijiao Wang1, Haibin Chen2*, Hongyan Yu1, Zexian Fu3 and Jianjun Zhao2*

... and immunohistochemical methods were used to detect the expression of EPAS-1 in the three groups. The expression of EPAS-1 and the clinicopathology of RCC were further analysed. To examine the relationship between these characteristics, a receiver operating characteristic curve (ROC) was used to evaluate the diagnostic value of EPAS-1 expression for lymph node metastasis in RCC. Compared with the adjacent tissue, the expression of EPAS-1 in RCC was significan...
Muhammad Fiaz Qamar1*, Tahir Hussain1, Iram Liaqat2, Madiha Kiran1, Abdur Rahman Ansari3, Faiza Yasmeen1 and Tahira Batool3
...rough routine laboratory methods in district Jhang. Fresh fecal samples (n = 1800) of pigeons were gathered in plastic bags from district Jhang home fanciers and local retailers. Smearing, sedimentation, and flotation procedures were among the various parasitological methods used to analyze all of the samples that were gathered. Herein, the prevalence of coccidiosis was 70.7% and that of capillariasis was 15.7% wherea...
Nauman Jamil Khan1, Rabia Tariq2, Hina Saleem2 and Muhammad Waheed Mushtaq2*
...troscopy, chromatography methods, ion selective electrode method, laser-induced breakdown spectroscopy, complexometric titration method and thermometric endpoint titrimetry are developed to determine the quantity of sodium. Spectrophotometric methods are cheap, easy to handle and more precise to quantify sodium in food contents.
...

Rahmat Elahi1, Gulnaz Parveen1*, Nazara1, Faryal Ali2, Nain Tara3 and Saba Iqbal1

...tter test and agar plate methods, a total of eight different species of fungi were isolated from five different maize varieties. In the blotter test method, only spores and mycelium of seed-borne fungi were detected, that was further confirmed by the agar plate method revealed the presence of eight different types of fungal colonies in maize varieties, each with varying percentages. Like Trichoderma harzianum colonies accounted for 3.33% of the treated seeds a...
Johan Sukweenadhi1*, Stefan Pratama Chandra1, Finna Setiawan2, Christina Avanti2, Kartini Kartini2, Arief Koeswanto3 and Deok-Chun Yang4
...synthesis is one of many methods that can be used to synthesize silver nanoparticles. Based on a statistical analysis of the average wound area, epidermal reconstruction, and TIME-H scores, significant differences began to occur from day 12 to day 14. The average wound area, epidermal reconstruction, and TIME-H scores in the diabetic mice group that were given a dermal patch formulated with silver nanoparticles synthesized using Plantago major L. extrac...
Hagag, N.M.*•, Arafa, A.*; Shalaby, M. A. ** El-Sanousi, A. A. **and Aly, M. M. 
...Application of molecular methods such as Reverse Transcriptase polymerase chain reaction (RT-PCR) and Real time RT-PCR (RRT-PCR) as a rapid, specific and sensitive detection methods currently used in national and reference laboratories worldwide. In this study a comparison of the specificity and sensitivity between 2 different formats of conventional RT-PCR (one and two steps) and 3 different formats of RRTPCR (one step usin...

Nasr-Eldin1 , M. A.; El-Dougdoug2, K. A.; Othman2, B. Ahmedi , Sabah A, and Abdcl-Azizl , S. Il.

...l indexing and molecular methods. In the present study, direct tissue-printing and dot-blot on the membranes, procedures has been applied on a large scale for an initial screening of HSVd, CEVd and PSTVd in Egypt. The results showed that the tissue print assays allowed clear discrimination between healthy and viroid-infected grapevine plants than dot-blot hybridization, the number Of HSVd-infected grapevine plants were 10 plants, PSTVd were 10 plants and the C...

Khatab, Eman A.H.; Zein, Salwa N. and Ahmed, Amal A.

...on using three different methods of injections. The titer Ofthe prepared antiserum as determined by indirect ELISA with dilution of tissues 1/5 was 1/1024. Purification of the immunogamaglobulin G and conjugate of IgG with alkaline phosphatase were carried out to be used for ELISA detection. The concentrations of IgG and IgG conjugated with alkaline phosphatase were 1.0 mg/ml and 1:1000 respectively. Antiserum produced specific to BBTMV was used for virus dete...

Manal A. El-Shazly1, A. s. 2 Abdel Wahab and Salwa N. Zein3

...t serological diagnostic methods such as indirect ELISA and dot-blot immunoassay (DBIA) on nitrocellulose membranes.

...

S. A. Sidaros*, S. A. EL-Kewey*, Eman A. H. khattab**, M. M. ELsharkawy* 

...vely using two different methods. The UV absorption spectrum had maxima and minima at 260 and245 nm, respectively for the two viruses. The absorbance ratios of A and A260/280 of the combined lower bands of purified BBSV preparations were 1.19 and 1.62, respectively. Infected faba bean plants yielded up to 0.48 mg/100g tissue. The corresponding data for CABMV were 1.20&1.22 and its yield was 3.52 mg/100g of cowpea leaves. The maximum antiserum titer against...

S.Y.M. Mahmoud1 and M. Hashem2

...norate. thus, precaution methods must be taken for reducing virus transmitting to other free-virus regions. 

...

A.A.Farrag;  I.A.M. Ibrahim and, H.M. Mazyad

... molecular hybridization methods. The result showed that it is more sensitive than DASELISA and can be used for large scale detection. PCR product was cloned and sequenced. Comparison between local isolate sequence and other published sequences of the Same virus shows 79.4% - 84% homology.

...

Rana Mohammed Ibrahim1*, Haider Mohammed Ali Al-Rubaie2

...olymerase Chain Reaction methods in Baghdad city between 1/11/ 2021 and 31/3/2022. Fifty jugular venous blood samples (about 5ml) were collected. The total infection rate of Leucocytozoon spp. in the blood smears was 10% (5/50), which divided into males 6% and in females 4%, while by qRT-PCR was 4% (2/50) with an equal ratio between males and females (2%). In conclusion, it is the first diagnosed study in Baghdad city to detect Leucocytozoon spp. in broiler ch...

Sulake Fadhil Al-Zubaidi1*, Ghusoon A.A. Alneamah2, Ali Saleh Mahdi1, Abdulraheem Abduljalil Wali3

... was to evaluate various methods of treatment of pyometra in bitches. For this purpose, the study was conducted in a private clinic and veterinary hospital in Baghdad. The study included seventeen different breeds bitches. The ages of bitches range from 12-132 months with an average of 38 months. Bitches are diagnosed with pyometra (open and closed) depending on clinical signs, ultrasound, and complete blood count (CBC). Bitches were allocated into four groups...

Makmun1, Imam Mujahidin Fahmid2, Muhammad Saleh S. Ali2*, Muhammad Yamin Saud2, Rahmadanih3

...e and efficient business methods.
 
Keywords | Actor, Actor relation, Egg production, Laying chicken, Blitar regency
...

Nurlan Akhmetsadykov1*, Tanatar Kydyrov2, Moldir Akhmetzhanova1, Gulnazi Akhmetova3, Maxat Berdikulov4 

...w serological diagnostic methods is highly relevant. The primary objective of this study was to produce a recombinant GM6 antigen derived from T. evansi and investigate its immunological properties for the detection and diagnosis of trypanosomosis. To create a recombinant antigen, bioinformatic analysis of the primary structure of T. evansi GM6 antigen from several regions was carried out. The protein sequence was reverse translated to the nucleotide sequence,...

Kouengoua Kouengoua Armelle Prudence1*, Yessinou Roland2, Nankam Chimi Roland1, Fotsac Dzousse Muller1, Kouam Alain1, Djuikwo Felicite1, Facho Balaam1, Awah Ndukum Julius3, Farougou Souaibou2 

...ce, host, causal agents, methods of diagnostics, risk factors. After several screenings, six articles were considered for this systematic review. East and Southern Africa countries carry on most researches on bovine tuberculosis in wildlife. Available studies assessed the status of wild mammal, carnivore. Further researches to improve the knowledge on the status of bovine tuberculosis in wildlife in Africa are still needed in order to allow proper disease moni...

Skeikh Mustafizur Rahman1*, Mst. Shirin Sharmin Khan1, Yousef Ahmed Alkhamis2,3, Roshmon Thomas Mathew2, Md. Moshiur Rahman1, Mohammed Monirul Islam4, Md. Asadujjaman5 and Md. Golam Sarower1

...56%) between species and methods of cooking varied considerably. The highest amounts of protein (19.18 to 33.55%) were found in the fried fish, while the raw and boiled fish contained almost similar amount (protein, 14.00 to 19.57%). Fat content differed between species ranged from 0.38 to 4% in raw fish, 0.78 to 3.43% in boiled fish, and 15.72 to 33.78% in fried fish. The ash content also differed among raw (1.05 to 3.76%), boiled (0.51 to 3.00%) and fried (1...

Ahokpossi AGAC1,2*, Salifou CFA2, Youssao Abdou Karim I2, Ameyapoh Y3 

...s. Regardless of farming methods, the meat of Goliath chickens was accepted and appreciated by the panelists.  

...
Syed Roohullah Jan1, Kareem Akhtar2*, Uroosa1, Muhammad Zeeshan Zahir3, Abdul Shakoor4
 
...erated using traditional methods undergo oxidation and contamination problems. Cold spray is recently used to obtain pure coatings without oxidation. In this work coatings of Ag-Sn-Cu powder are achieved on the Aluminum substrate and human teeth, using the high-pressure cold spray technique. The powder when mixed with Mercury is called amalgam and is commonly used in dental applications for cavity filling. However, Mercury is health hazardous. In the present s...

Siqiang Li1,2, Tiantian Wang1, Peng Sun1, Airong Gao1, Xin Gong1, Yuanhong Xu2, Baogen Wang2, Jun Wu1* and Bo Liu1*

...tional molecular cloning methods, and expressed in a 5-liter fermentation tank. The target protein was purified in three-step purification and identified by peptide mass of fingerprint. The enzymatic activity and optimal reaction conditions of MDS I were detected using DNA sequencer-assisted fluorophore-assisted carbohydrate electrophoresis. We obtained MDS I with a purity exceeding 90% in gram scales, which was capable of digesting α-1,2 linked mannose ...

Khaeruddin1, Gatot Ciptadi2, Muhammad Yusuf3, Iswati4, Setya Budhi Udrayana4, Sri Wahjuningsih2*

... using abdominal massage methods and divided into 4 tubes filled with diluent. For the analysis, the diluent selected was egg yolk Ringer’s lactate added with BHT at concentrations of 1, 2, and 3 mM, respectively as well as a control treatment without BHT. Liquid semen was stored for 72 hours at 5°C, with spermatozoa quality monitored every 24 hours in 10 replicated experiments. The results showed that sperm motility, viability, and plasma membrane i...
Yongtao Xu1, Dandan Wang1, Xiaolong Hu1, Minling Li1, Ming Tang1, Wuhua Liu2, Jianwen Zhan2 and Weiwei Zhang1*
...ed with traditional diet methods. This study assembled basic summer diet data and provided an important accumulation for the protection and monitoring of Taohongling sika deers, which also provides reference for the establishment of local database in the future.

...

Wael Halawa1, Raed M. Al-Atiyat2*, Jamal Abo Omar1, Faisal S. Rashaydeh3, Fath Ullah4, Shakirullah5 and Berkant Ismail Yildiz6

...ct of different suckling methods on newborn Assaf lambs growth performance and mortality. A total of 97 lambs were used in the study, with lambing periods starting from October 2020 to January 2021. Ewes’ parities were first, second, and third. After lambing, lambs were randomly distributed according to the suckling method into three groups: natural suckling (G1) (39 lambs), lambs fed completely with milk replacer (G2) (29 lambs), and lambs fed milk repl...

Zhenglei Qiao*, Jie Xing and Fang Li

...e and maximum likelihood methods. We found that P. stoliczkana was closely related to Pethia ticto and Pethia padamya. These results enrich the mitochondrial genome database of Smiliogastrinae fish and provide reference materials for systematic classification of this group of fish.

...

Lijuan Liu1, Jinghang Chen2*, Min Wang3 and Wei Li4

... systemic administration methods such as oral or injection, inhaled medications are directly administered to the respiratory tract to exert a therapeutic impact, which has clear benefits in the treatment of respiratory illnesses. However, existing inhaled medications are difficult to fulfill clinical prescription demands, and the research and development of novel inhaled pharmaceuticals has significant hurdles due to a lack of comprehensive theoretical and pra...

Ghaith Z. Hasan Al-Askari *, Eman H. Yousif Al-Taee

...sues of female rats. The methods included in the first experiment, the female rats were orally administered the following lethal doses: 0.5, 1, 1.5, 2, 2.5, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, and 16 mg/kg b.w. of vitamin D. Survival and mortality numbers were observed following 24 hours of intoxication. In the second experiment, the selected groups were divided according to the doses they received. In a cross-sectional study, 30 female rats were divi...

Taghreed Jabbar Humadai*, Bushra Ibrahim AL-Kaisei

...in male albino rats. The methods included sixty male albino rats at age 8-9 weeks and weighted 190-200 gm were divided into three groups: The first group: Consisted of 20 rats control were given distilled water and normal rat pellet, the second group: Consisted of 20 rats injected weekly 0.5µg /kg. b.w. by I/P route which represented 1/20 LD50 of tetrodotoxin, the third group: It also consisted of 20 rats injected weekly 1 µg /kg. b.w. by I/P route...

Hashim Hadi Al-Jebory1*, Mohammed Khalil Ibrahim Al-Saeedi2, Majeed Ajafar1, Nihad Abdul-Lateef Ali1

...ct of various levels and methods of adding melatonin to water and diets on the growth performance of broiler chickens.  One day old, 400 Ross 308 broiler chicks were randomly allocated into five random groups, with each group being quadruplicated and 20 birds allocated to each replication. Melatonin was supplemented in the G1 control group. G2 received 10 mg of melatonin per kg of diet, G3 received 20 mg of melatonin per kg of diet, G4 received 10 mg of m...

Nadya Indriyani1, Sri Murtini2*, Sri Darwati3, Cece Sumantri3

...le disease virus (AbNDV) methods using Hemaglutination Inhibition (HI) tests. Descriptive analysis was performed on production performance data and humoral immunity to estimate phenotypic correlation of the statistical method of Pearson correlation analysis. The results of IgY examination in men aged 27 weeks were 9.49 ± 1.37 mg/mL and IgY in women 9.36 ± 2.89 mg/mL. AbNDV ages 0 and 2 weeks at 1.34±0.95 and 1,370.95 LOG 2 GMT. At the age ...

ZOHAIB SAEED1, SHAHID IQBAL*1, UMER YOUNAS1,2, MUHAMMAD PERVAIZ*3, SYED MOHSIN ALI NAQVI3 & RANA RASHAD MAHMOOD KHAN3

...ntalists. There are many methods for its disposal. The most suitable and economical method is its land application. Lagenaria siceraria L. plant was grown in various amendments of soil and sewage sludge to check the tolerance of the plant and to select the optimized mixing of sewage sludge with soil for this particular plant. Estimation of biochemical parameters like chlorophyll content, carotenoid content, and protein content along with total biomass of the p...

ASMA WAHEED QURESHI1* & SHUMAILA ALAM2

... assessed using standard methods. Overall parasitic contamination of 30.7% was observed. Highest contamination was detected in Mardan (36%) followed by Katlang (30%) while the lowest contamination was observed in Takhat bhai (26%). Hookworm (32.6%) was the most common contaminant found followed by Ascaris lumbricoides (21.7%) and Trichostrongylus spp. (10.87%) and the least detected parasite was Schistosoma (1.08%). Among vegetables, lettuce and mint showed hi...

*SHAJAHAN BAIG1, MUSHTAQ AHMAD SALEEM1, MUHAMMAD AZMAT ULLAH KHAN1 ZEESHAN NADEEM2, SUFIAN AHMED2, MEMUNA GHAFOOR SHAHID3 & TANZEEM AKBAR CHEEMA3

...cs and protein profiling methods. These profiling methods are very useful but still studies on sensitivity, specificity and substantiation are needed. Furthermore, bioinformatics studies may be quite useful for the successful application of these profiling methods to analyze the safety of genetically modified (GM) foods. These bioinformatic methods emplo...

ASMA ZAFAR*, MONIBA KHAWAR, MAHNOOR CHAUDHRY, SAYYEDA SANA BATOOL, AMJAD HUSSAIN** & MUSHTAQ AHMAD SALEEM

...d of medicine. Different methods are being used to eradicate cancer including chemotherapy, radiotherapy, immunotoxins, and drug therapy but these treatments cause various side effects to the cancer patients, as they not only target cancer cells but also cause harm to normal body cells. At present some live bacteria, bacterial products, and bacterial proteins are used to treat cancer. One of the amazing bacterial protein azurin can work as an anti-cancerous ag...

UMER YOUNAS, SHAHID IQBAL* & JAMSHED AKBAR

...lts revealed that drying methods have no significant influence on recovery of antioxidants from sample matrix. However, methanolic extracts exhibited highest antioxidant potential that confirmed superiority of methanol as extraction media over the other solvents used. In addition, microwave-assisted extraction technique for the recovery of antioxidants from M. nigra leaves was optimized using response surface methodology. For the purpose, oven dried samples we...

1*AFIRA WAQAR, 2ADNAN RAUF, 3BUSHRA CHAUDHRY &4IKRAM-UL HAQ

...and the most appropriate methods for genetic analysis.

...

Sri Wahjuningsih1*, Ani Atul Arif1, Khaerudin2, Herlina Pratiwi3, Ardyah Ramadhina Irsanti Putri1

... collected using massage methods. This study uses a complete random design of factorial patterns with factor I being the equilibration time and factor II being the different thawing conditions. Fresh semen was evaluated macroscopically and microscopically. The sperm used had a motility of over 80%. The semen was diluted Lactate Ringer with 10% egg yolk and the addition of 7% DMSO cryoprotectant. Then the semen was equilibrated at different times and then froze...

Putri Okta Shafura1, Mardiati Zain2*, Elihasridas2, Bima Bagaskara1, Ummi Amanah1, Laras Sukma Sucitra1, Bella Veliana Utami1, Roni Pazla2, Erpomen2, Ezi Masdia Putri3, Rusmana Wijaya Setia Ningrat2, Riris Delima Purba3, Ruslan Abdul Gopar3, Putut Suryo Negoro3

...illey and Terry in-vitro methods were utilized to measure rumen characteristics, nutrient digestibility, protozoan population, microbial protein synthesis, and methane gas production. The results revealed a significant impact (P < 0.05) of these treatments on nutrient digestibility and rumen fermentation characteristics. The combination of 0.5% S. cerevisiae and 0.3% sulfur tended to increase nutrient digestibility, total VFA production, and microbial prote...

Waseem Abbas, Imtiaz Ahmed* and Imran Khan 

...performed using standard methods. In-vitro starch digestibility and total starch were determined by Megazyme starch assay kit. A nine-point hedonic scale assessed bread samples for consumer acceptance. The findings of proximate composition showed a significant increase in ash at 2 and 3 % incorporation. The fiber content also significantly increased at the incorporation ratio of 1, 2 and 3%. In CWB, fat concentration at 2 and 3% incorporation remained almost e...

Muhammad Wasif Gulzar*, Riffat Maqsood, Muhammad Zain, Muhammad Suleman, Tayyab Ur Rehman, Sana Asif, Abdul Wadood and Jawad Hussain

...n tissues. The preferred methods for routine diagnosis include PCR (Polymerase Chain Reaction), and Mouse Inoculation Test (MIT). In endemic locations, vaccinations with DNA, recombinant vaccines, and live, attenuated, or inactivated viruses can be administered. This study provides comprehensive information on immunization, treatment techniques, pathophysiology, epidemiology, transmission, and relevant preventive and control measures.

...

Yousef Abdal Jalil Fadladdin1, Ateeq Ullah2*, Raheela Nawaz3, Yagoob Garedaghi4, Abbas M.A. Al-Azab5 and Mashael Abdullah Aldamigh6

...rocessed in direct smear methods and examined under microscope first under low power objectives and then higher power Lense. Evidence of infection was noted by the presence of helminth eggs. Out of 200 samples 65% (n=130) were found to be infected with 2 species of soil transmitted helminths including 27% (n=54) A. lumbricoides and 35.5% (n=71) A. duodenale. Regarding ages, 11-12 years were highly infected. Females were observed to be infected more than male s...

Aspen Abutalip*, Batyrbek Aitzhanov, Assiya Mussayeva, Vladislava Suchshikh, Natalya Yegorova

...es a constant search for methods aimed at its elimination. One such approach is the timely and rapid diagnosis of the disease, which will allow the timely implementation of preventive measures. The purpose of the study was to develop an express test to identify cases of animal morbidity for emphysematous carbuncle in the field. For this purpose, a serological method was used using the latex agglutination test (LAT). When preparing the diagnostic, antibodies to...

Shangmei Peng1 and Changhe Yu2*

...in different examination methods. There was no significant difference compared with that in contrast-enhanced CT group (4.90%, P>0.05). The serum creatinine, uric acid and urea nitrogen in the contrast-enhanced CT group decreased, while cystatin C and eGFR increased after the examination, with statistical significance (P<0.05). But there was no significant difference in creatinine, urea, urea nitrogen, cystatin C and eGFR between the two groups at the sa...

Hilarius Yosef Sikone1*, Gomera Bouk2, Edelnia Kristina Bere2, Yohana Kamlasi2, Elisabeth Yulia Nugraha1

...This research used mixed methods to obtain qualitative and quantitative data, a survey was conducted by 180 breeders from 18 respondent groups. Respondents must meet two requirements: have raised Bali cattle for at least five years and own at least three cows. A basic random sampling technique was employed to identify the sample, which involved selecting sample members randomly from among farmer respondents in six sub-districts without considering strata. The ...
Luqman1, Zahid Hussain1, Mohammad Ilyas2, Azmat Ali Awan3, Javed Rahman 2,
Mazhar Ali2 and Tamana Bakht4
...e="text-align: justify;">methods in bitter gourd. The treatments comprised of four mulches (i.e. Rumex crispus,
Silybum marianum, newspaper as mulch, and saw-dust as mulch), a herbicide i.e. Stomp
330 EC (pendimethalin) applied as pre-emergence. For comparison of the treatments with
weed free and weedy conditions, a hand weeding and a weedy check (control) treatment
were also kept. Data were collected on weeds fr...
Haseeb Ahmad1*, Muhammad Shafi1, Waqas Liaqat1, Muhammad Faheem Jan1,
Shahzad Ahmad2 and Muhammad Farooq3
...tillage and weed control methods was conducted at Agronomy Research Farm, The
University of Agriculture Peshawar during summer 2016. Field experiment was conducted in
randomized complete block design (RCBD) with split plot arrangement having four
replications. Tillage practices i.e. chisel plough + rotavator, mouldboard plough + rotavator,
cultivator + rotavator and rotavator were assigned to main plots. Weeds ma...
Imtiaz Khan,1 Muhammad Kabir, Muhammad Ishfaq Khan, Haroon Khan, Saima
Hashim and Muhammad Azim Khan
... performance by adopting methods of sowing and mixed tank herbicides
application. The experiment was arranged in split-plot with randomized complete block
(RCB) design with 4 replications. For practical adaptation of the experiment, the sowing
methods were allotted as the main plots, while tank mixed herbicides were kept in the subplots
to get prominent results for the possible...
Muhammad Saleem Chang*[1], Muhammad Nawaz Kandhro2, Zulfiqar Ali Abbasi4, Jay Kumar Sootaher*3, Piar Ali Shar3, Kashif Ali Buriro2, Nadia Mangrio5, Zafarullah Channa3
 
 

Muhammad Ather Nadeem1, Bilal Ahmad Khan1* Sadia Afzal2, Rizwan Maqbool3, Hasnain Waheed1, Aneela Nijabat4, Muhammad Ikram5, Amir Aziz6, Muhammad Adnan1, Qaisar Mehmood7, Hasnain Umer3

... controlled by different methods among them use of aqueous extracts of crops is an imperative method. In this method allelochemicals present in crop plant help in suppressing or inhibiting weeds. Therefore, this study was planned to investigate the allopathic potential of aqueous extract of winter crop Papaver somniferum (opium) on summer weed Echinochloa cruss-galli (barnyard grass. The experiment comprised of seven concentrations (0, 0.25, 0.50, 1, 2, 4, and...

Mthi S. 1,2, Ikusika O.O.1*, Washaya S.3, Mpendulo C.T.1, Nowers C.B.2 

...aster and modified Stoll methods, while body weight was measured using an electronic scale. Treatments had a significant difference (P<0.05) in the weight gain of lambs. Improvement in the average daily weight gain performance of lambs was also observed at 10% DME inclusion levels, followed by T1 (31.96±7.787 kg), T2 (31.86±7.787 kg), and T3 (31.5±7.787 kg), respectively. Coccidia and roundworms were the only species found in the collec...

Mansoor Ali Khan*, Khalid Imran, Zahid Rauf, Tanvir Hussain, Muhammad Umair Khan and Sajid Ali

...) and spectrophotometric methods were used for sample analysis. Results from the Guava (Psidium guajava) revealed that moisture contents (80.16%), protein (2.52%), fibers (2.22%), phosphorus (1.47%), iron (80.20PPm), Vit-C (1.36%), total phenol contents (180.00mg/100g), calcium (11.00mg/100g), magnesium (16.50mg/100g), manganese (0.12mg/100g) and potassium (373.20mg/100g) were maximum in the famous Kohat variety of guava. Alkaloids (0.60%), pectin (0.10%) lipi...

Ghalib Nadeem* and Muhammad Irfan Anis 

... information sources and methods is widely applicate in the field of bovine disease prevention and health monitoring. Bovine disease detection is an emerging subject today to accomplish the farms demands of individuals across the globe. This research delves into the realm of bovine disease and event detection using advanced Machine Learning (ML) techniques. Focusing on the critical events of estrus, acidosis, mastitis, lameness, and calving, our study aims to ...
FAZAL MUNSIF,Ayeni Leye Samuel,ALI SHAN,ZAHID ALI,SHAHEN SHAH,Muhammad Amin,Akhlaq Ahmad
HABIB UR REHMAN,MUHAMMAD SADIQ,MUHAMMAD ANWAR KHAN,Kalim Ullah,MUHAMMAD ZUBAIR,ABDUL MATEEN KHATTAK
FAZAL MUNSIF,IMRAN KHAN,KAWSAR ALI,Zahid Hussain,Naushad khan
Abdullah, Gul Hassan, Ijaz Ahmad Khan, Sheraz Ahmad Khan , Hussain Ali
TABASSUM YASEEN,MASOD AHMAD,KAWSAR ALI,Abdullah,WAHEED ULLAH,NAVEEDULLAH,Md. Abdullah Al Mamun,Muhammad Amin
HAQ NAWAZ MALIK,Muhammad Aslam,MUHAMMAD AZAM KHAN TAREEN,HABIB IQBAL JAVED
MOHAMMAD JAMAL KHAN,NAZIR AHMAD,AZHAR RAUF,MOHAMMAD AMIN,MOHAMMAD TARIQ JAN,MASOOD UR REHMAN
Muhammad Sarwar Cheema,MUHAMMAD SALEEM AHMAD,MUHAMMAD AFZAL,Muhammad Aslam
Muhammad Aslam , Haji Khalil Ahmad , Ejaz Ahmad , Himayatullah , Muhammad Ayyaz Khan , Abdul Ghaffar Sagoo
Bakhitar Gul,K.B. Marwat,Haroon Khan,M. Azim Khan,MUHAMMAD FAWAD
Muhammad Sarwar Cheema , Muhammad Akhtar , Muhammad Sarfaraz Iqbal
Masood Ur Rahman,Muhammad Hanif,Muhammad Amin,Salim Shah,Subhan Uddin,SAJJAD ALI,Rafi Ud Din,Anwar Ali Shad
A. TANVIR,S. AHMED,A. MAHMOOD,Z. A. CHEEMA
KAWSAR ALI,Zahid Hussain,IFTIKHAR UD DIN,SHAD KHAN KHALIL,FAZAL MUNSIF,WAGMA
Zaid Khan, Bashir Ahmad, Habib Akbar, Muhammad Ayoub Khan , Sajjad Ahmad, Hafeez ur Rahim, Barkat Ali and Laiq Zada

Muhammad Bilal Zia1*, Ashar Farooq1, Hammad Ud Din1, Barkat Ullah Khan1 and Samee Ud Din2

...ng stock and propagation methods for Paulownia indicated that the root stumps grew substantially taller, having straight stems and symmetrical crowns. Highest survival was observed for Paulownia fortunei, Paulownia tomentosa and Paulownia elongata. Paulownia fortunei and Populus deltoides; both had comparable growth in diameter and physico-mechanical properties. Apart from its utilization in wood works, its scope is also emerging in pulp and paper industry. Us...

Wei Li1, Xvming Qi2, Xueli Lu3, Li Yuan4 and Huaiqing Deng1*

... by through non-invasive methods, and the levels of progesterone and testosterone were analyzed by high-performance liquid chromatography mass spectrometry. The results showed that the menstrual cycle of adult females in Hainan gibbon group C was 32±3.6 days and the duration of the menstrual period was 10±2.4 days. There was no significant difference in progesterone levels between individuals of different age and sex classes, no significant perio...

Lipigwe Lauya1*, Peace Nkiruka Okeke2, Nanma Tongnan Cosmas1 and Chukwudi Chizorom Ibeh1

...e; inadequate diagnostic methods and algorithms for monitoring HIV-2, the possibility of dangerous genetic mutations and resurgence of super-strains, cross-reactivity of HIV types that interfere with accurate reporting of HIV-2 cases, and suboptimal/neglected treatment and care (S/NTC) for HIV-2 individuals. Additionally, gaps in surveillance infrastructure, health inequality in classifying HIV burdens based on HIV type, and deprived HIV-2 research and policy ...

Basheer Ahmad, Nowsherwan Zarif, Saif Ullah Khan, Salman Ahmad and Anwar Ali*

...ng sustainable plans and methods.

...
Salman Ahmad1, Anwar Ali1, Basheer Ahmad1, Nowsherwan Zarif1* and Saif Ullah Khan1
...op long-term sustainable methods and methodologies are recommended. This study was conducted in District Malakand to learn the effects of agroforestry practices on farmers and the significant reasons for planting as well as the primary advantages obtained from agroforestry in order to comprehend the effects of agroforestry practices on farmers in Tehsil Thana, District Malakand, KP. A structured questionnaire was used to gather the necessary data from 40 respo...
Muhammad Umair1, Nowsherwan Zarif1*, Zahid Rauf1, Anwar Ali1, Ghayyas Ahmed1, Basheer Ahmed1, Salman Ahmad1 and Saifullah1
...ting systems and develop methods and approaches that are sustainable over the long run-in order to improve agroforestry in Pakistan.
Keywords: Agroforestry, Sustainable, Intercropping, socioeconomics, sustainable economics.
...
Nasim Abbas, M. Yousaf Khan, Tanvir Hussain and Muhammad Zahid
...n by standard laboratory methods. Permanent slides of cross, radial and tangential sections of the wood were prepared to determine anatomical properties of the wood through microscopic observations. The wood samples were prepared and tested as per international standard to determine physical and mechanical properties of wood. The thick walled and long fibers were observed which showed its better strength. The wood may be less durable or non-durable, however, c...
Naveed Ahmed
...ainst termites using two methods of application, soil treatment and wood treatment at Pakistan Forest Institute (PFI), Peshawar and Punjab Forest Research Institute (PFRI), Gatwala, Faisalabad. Concentrations of 0.5%, 1.0% and 2.0% of each termiticide were applied for soil and wood treatment. Result indicated that after 50 months of treatment to wooden stacks, Tenekil Plus 100% E.C. gave 98% and 90% protection in 2.0% concentration at Peshawar and Faisalabad, ...
Mian Muhammad Shafiq and Hussain Bux Bhaagat
...dlife staff) observation methods were used.

These lakes support a good population of waterfowls, Marsh crocodiles, otters and fish stock. About 1% of global population of globally threatened duck specie i.e. Marbled teal (Marmaronetta angustisvostris) breed in these lakes in May-July. According to the data Deh Akro II wildlife sanctuary provides good wintering ground to the migratory waterfowls, as there was least disturbance. Moreover, this ...

Iqbal Mahmood, Gul Rukh and Tanvir Hussain
...d by standard laboratory methods. .The results of the properties have been compared with the same species found in Burma. On the basis of result of properties the locally grown Teak wood is not only superior in strength but also suitable for various purposes where high stress is required. The local Teak wood has favourable relationship between tangential and radial shrinkage and aptness for framework and similar articles where the dimensional stability is o...
Sarfraz Ahmad, Sarwat N. Mirza and Muhammad Islam
...ween drill and broadcast methods. The seedlings of S.vermiculata were able to survive and attain some growth under extreme drought conditions and withstand minimum temperature of -11oC. The initial trial on emergence and survival indicates that S. vermiculata possesses characteristics of drought and cold resistance and has adaptability to be grown under arid conditions of highland Balochistan....
Mian Muhammad Shafiq and Muhammad Idrees
...angri, Dogazza and Patti methods are used for the live catching/trapping of migratory falcons in these areas.

The Government of Pakistan has signed International Conventions like CITES, CMS etc. According to the CITES convention Appendices list (www.cites.org) the peregrine falcon is placed in the list of in Appendix l and Saker falcon is in the Appendix II. Whereas both these species are included in the list of Appendix II of the CMS.

...
Ajiboye, A. A.1, Ebofin, A. O.1, M. O. Atayese,2, M. O. Adedire3, D. A. Agboola1 and M. Kadiri1
... species. Some standard methods of releasing seed dormancy were used. Percentage germination range in fresh seeds of P. africana and D. guineensis was 0-10%. The seeds of the two tree species exhibit physical dormancy due to hard seed coat. Dormancy in P. africana and D. guineensis was terminated by soaking of seeds in 90% concentrated sulphuric acid for 10-15 minutes; and mechanical scarification using coarse sand, gravel and e...
Sardar M. Rafique and Ghulam Ali Bajwa
...) more than conventional methods of rearing. In case of 206-PO, return of larval body weight gain, cocoon and shell weight were 8.15%, 11.76% and 9.56% higher, respectively. Larvae transported in wax paper lost larval body weight by 4.24% (C-102) and 4.09% (206-PO) as compared to wooden rearing tray. Combination of motorbike and wooden rearing tray produced maximum larval body weight (3.23gl-1), cocoon weight (1.53g), shell weight (0.32) and cocoon ...
Ghayyas Ahmad
...dge-based classification methods. Patch size of the remaining semi-natural forests was selected as the most important criterion for the identification of priority areas. However, patch shape index was also measured.

NDVI (Normalized difference vegetation index) was found to be a good predictor of forest vegetation of the area, particularly, when remote-sensing data is collected during summer rainy season as in this study. Results of image classification ...

Siraj-ud-Din and Abdul Waheed Baloch
...to conventional planting methods. Within the various water conservation techniques contour trenches and earthen moistures are more economical and are able to keep the period happy and vigorous for a longer period of time. Biological treatment to sand dune stabilization is the only permanent and everlasting solution to this problem.
...
Iqbal Mahmood and G. M. Nasir
...accordance with standard methods. The results indicated that the wood of Cedrela toona is good in all the physical and mechanical properties both in green and air dry conditions and meet the standard requirements. More diversified and extensive use of the wood can be contemplated in addition to its traditional uses....
Hanif Gul, G. A. Bajwa and G. N. Panhwar
... mechanical and chemical methods were incorporated. During November - December 1.5 to 2.0 meter area around each previously infested trees of Jacranda mymosipholia and Paulownia spp. were hoed and in following February sticky bands were applied on stems at breast height level. For chemical control two experiments were carried out in RCBD. In first experiment five insecticides, i.e. Buldock 25 EC, Endon 35 EC, Fenvelrate 20 EC, Mepra 50EC ...
Ghulam Akbar, Muhammad Arshad and Taj Naseeb Khan
...eding and stump planting methods of five grasses were compared at Arid Zone Research Institute (PARC) farm in Cholistan during monsoon seasons of 1992-94. A seed rate of 250 seeds/m2 was used in case of seeding while for stump planting the plant to plant spacing was kept at 0.5 m. Overall dry matter yield (DMY) than rest of the grasses. Field observations confirmed that plants emerging from seed were morphologically more vigorous than those ...
Muhammad Hafeez, Liaqat Hussain Jafri and Mohammad Rafiq
...onditions by two common methods i.e., direct sowing vs tubed planting, an experiment was laid out in Shakaragarh Tehsil during July, 1987. Costs incurred on raising of Acacia nilotica by two methods was calculated. Results indicate that although the method of direct sowing is easier and apparently cheaper but in the long run, the method of raising kikar woodlots on farm lands by planting tubed plants is prefer...
Hanif Gul
...agement of termites with methods other than the conventional chemical control. The new concepts in addition to chemical control for build up of a model of an integrated management of termites were population assessment, wood resistance trails, resistant wood extractive trials, influence of plant stress on termites infestation. Population assessment trial carried out in different localities showed the species wise distribution along with their strength....
Mohammad Rafique Sardar
...lanted under 5 planting methods viz; Surface planting, pit planting, trench planting, slope planting and slope with trench planting in Peshawar during August, 1983. The main objective of the study was to test the survival and growth performance of fodder trees under arid conditions. Results indicated that survival and establishment of L. leucocephala was highest (87%) of all other 5 trees species. A. albida had second best survival with ...
K. M. Siddiqui
...ons were using tools and methods of forest contractors because there were no arrangements within the country to train the staff in improved methods of tree felling, conversion and transportation and for conducting research to find out the best methods for the local conditions....
Mohammad Ayaz and Gerhard Stohr
...ansportation system. The methods and means of timber harvesting not only determine the magnitude of material to be extracted and economic gains of the operation but also the preservation of remaining stand and site....
Mahmood Iqbal Sheikh
...for finding out suitable methods of afforestation in arid and semi-arid areas of Pakistan, some studies have been conducted in Rakh Dagar Kotli (Thal desert of Pakistan) using different methods of site preparation and tree species. The water harvesting methods used i9nvolved preparation of trenches with and without catchments, sloping catchments without a trench, pits and flat groun...
Mohammad Afzal Cheema and Michael Kleine
...of one of the following methods till 40 years back....
Mahmood Iqbal Sheikh
...fties. Through the years methods of growing this tree on a large scale have been perfected and particular clones which would successfully grow under different climatic conditions have been identified. However, the optimum growing space requirements of poplars still remained to be standardised. Such a study had a special significance in Pakistan in view of the tremendous shortage of raw material for various end uses and a final decision was required to be t...
M. I. Shiekh, R.W. Hussain and Saliheen Khan
...s 58.16 million kg. Four methods are adopted for tobacco curing i.e. air, sun flue and fire and fire drying. The tobacco crop grown in NWFP is 15,000. Data were collected from 58curing units throughout the province. The average loading capacity of green tobacco per unit is 1500-2500 kg. The average consumption of fire -wood per sample unit is 1.26 m3 per load and 6 to 7 days are required to cure one load. The average price of fore wood is 20/-, 28/-...
Gerald Foley
...introduce more efficient methods of charcoal making; most have been failures. Some development experts now say charcoal making should be discouraged. The Gambia has imposed a ban on charcoal making.

Charcoal Making in the Developing World is essential reading for anyone concerned with the problems of woodfuel and deforestation in the developing world. It is the most comprehensive publication available on the techniques of charcoal making, the physics fi...

Mahmood Iqbal Sheikh
...suited to poplars. Using methods have been initiated. Sports goods industry is the main user of willow wood. Biomass research on Salicacea is comparatively new to Pakistan. It has been found that different poplar clones grown under the same set of condition yield different quantities of biomass. ...
Mahmood Iqbal Sheikh, Ali Akbar Khan and Mohammad Khan
...te a few years back, the methods adopted in actual practice left much to be desired. Under the Hazara Preinvestment Project, World Bank authorities desired that in view of the extensive planting programme involved in the project it would be better if these practices were standardized for the benefit of the field foresters. The job was assigned to the Pakistan Forest Institute....
G. M. Khattak and Ashiq Ahmad
...flotation in butenol....
Mahmood Iqbal Sheikh and Abdul Aleem
...w it artificially. Usual methods are: planting one year old bare rooted nursery raised stock, and dibbling of seed. In the Murree Hills, dibbling of seed has been the usual method with poor results. This investigation was started to find the best method of growing walnut in that area....
Anwar Ahmad Khan, Munawar Sultana and Shakeel Haider Zaidi
...al rain fall) to develop methods for growing , rhizomes were collected in November from Murree and stored for one and two months at Peshawar in1:1 soil and sand mixture at one meter depth and planted after the storage period. Two months storage period gave significantly high (at 5%level) sprouting as compared to one month storage. Planting of full size rhizomes were tested against rhizome pieces (5, 7.5 and 10 cm). though full rhizo...
M. A. Quraishi, A. Khalique, Shahida Perveen and Perveen Akhtar
...the host. To develop new methods of control efforts are being made all over the world to collect basic physiological information about the host-parasite relationship. The present investigation is in the same direction and aims to explore the diurnal fluctuations in water balance parameters of this mistletoe in relation to those of its host, under natural conditions. ...
Taj Ali Wazir and S. M. A. Shah
...s. A number of different methods of retting were investigated to get at the optimum procedure. The anatomical characteristics, length, diameter and ratio of length to diameter of the ultimate fibers were evaluated. The effects of stage of harvesting, methods of retting, different levels of relative humidity, and anatomical characteristics on the strength of the filaments were examined. ...
M. B. Shrivastava and B. Ulrich
...Sites Evaluation and the methods used to solve them so far. The chief components of Eco-Systems determining the growth potential of forest lands have been discussed. The dependent variable of site quality exhibiting real difference in site productivity has been suggested. An easily applicable and accurate empirical method of estimating the potential productivity of the forest lands regardless of the kind of or lack of vegetation has been suggested. The...
Moin Uddin Ahmad and S. A. Qadir Dr.
... by quadrat and plotless methods. Community attributes, maturity and homogeneity of every stand were studied. 10 communities were recognized on the basis of physiognomy, floristic composition and importance value index....
Mahmood Iqbal Sheikh
...sistent effort, the best methods of growing and managing these species have been developed over a period of time. Since enough state land is not available to practice poplar cultivation with intensive agriculture, the farming community has been encouraged and persuaded to grow this tree on their land in the form of widely spaced compact blocks or row plantations. The targets which to many looked rather ambitious in the beginning, have fully been achieved throu...
Anwar Masrur, PFS (I)
...by means of quantitative methods instead of purely qualitative and deductive studies. The paper describes some aspects of morphometric analysis of Kunhar river watershed including stream order, bifurcation ratio, drainage density, constant of channel maintenance, stream frequency, shape, slope and elevation....

Kashif Shehzad* and Ikramul Haq

...ogy, sustainable farming methods and climate-resilient practices.

...

Ahmad Shandookh Hameed1, Ali Abd Kadhum2 and Murtada Wafi Beden1

... traditional cultivation methods along with antibiotic susceptibility testing for cultivated microbes. Using online sequential organ failure assessment score calculator to predict the outcome of the patients involved. A total of 41 patients the in I.C.U. suffers ventilator-associated pneumonia in this study, of whom 23 were infectious and 18 were non-infectious. The infectious group had higher sequential organ failure (SOFA) scores (p<0.05) and more Acineto...
Abdul Basit1, Ayesha Zahid1, Syed Tanveer Shah1, Inayat Ullah2, Sana Ullah3*, Muhammad
Kashif Nawaz4, Muhammad Areeb Khalid1, Izhar Ullah5, Fawad Ali1, Shaukat Ali1
...ifferent types of sowing methods (ridges and flat beds), and reduced to various number of
picking (3, 4, and 5 intervals). The results of the present study revealed that okra plants
sown on the ridges recorded maximum germination percentage (96.9%), fruit weight
(22.41g), pod length (14.44 cm), seed pod-1 (48), 100 seed weight (6.41 g), seed yield
(102.2 kg ha-1) and fresh yield (0.165 tons ha-1). Data regarding ...

Abdul Basit1 and 2Sana Ullah*

... and various
methods practiced by the gardeners to manage their nurseries, and distribution of
ornamental plant species. The main objective was to improve the current situation of the
nurseries and suggest recommendations in light of the highlighted problems. Various
attributes i.e. identification of plants, total area of nurseries, soil type, irrigation system,
weeding, fertilizer application, propag...

Ke Sun, Guangwei Fan, Yujie Zhang, Ji Luo, Xiaobing Wu and Tao Pan*

...ed and compared with two methods based on Otsu’s algorithm and iterative algorithm, respectively. The results show that the accuracy of the method based on the adaptive bimodal threshold segmentation algorithm is the highest (absolute error: 0.018±0.016) among these three methods. Please note that the accuracy is higher for images captured on cloudy days as compared to the images captured on sunny days. Moreover...

Muddassir Ali, Fraz Ahmad Khan*, Mohsin Ali Raza, Muhammad Shahid Munir and Muhammad Usman Saleem

...fferent paddy harvesting methods in terms of grain losses and head rice yield. This research evaluated the three harvesting methods: a Head-feed harvester (model PRO-588I-G, Kubota, Japan), a Whole-stalk harvester (model 8060, NH, Italy), and manual harvesting. The experiments were carried out on an area of 12,060 m2 with a complete randomized design (CRD) and three replications during the year 2021. The yield-related crop p...

Muhammad Nadeem1, Jamshaid Iqbal2, Muneer Abbas1*, Niaz Hussain1, Muhammad Tariq Javeed1, Abdul Ghaffar1, Muhammad Irshad1, Muhammad Aslam1, Gul Rehman2 and Shahar Yar Ahsan1

Md. Maniruzzaman1, Md. Kamrul Haque1,3, Md Rokonuzzaman2, Md. Mahmudul Hasan3, Rumana Biswas4, Md. Mustafizur Rahman5, Tahmina Akter Rimi6, Md. Rahat Uz Zaman7*, Md. Alauddin8, Md. Abdul Baki9 and Md. Yeamin Hossain10

...ines several alternative methods such as food processing, exporting to other countries, and involvement of GOs-NGOs and research institutes with the overall production system in terms of training, information transformation, and market research. The pandemic disproportionately affected various sectors of the Bangladesh economy. In this dark moment, agribusiness has shown a little light. The Coronavirus has created many opportunities in this sector that can hel...

Hafiz Muhammad Usama Shaheen1, Nasir Ahmed Rajput1*, Muhammad Atiq1, Ghalib Ayaz Kachelo1, Hadeed Ahmad1, Muhammad Wahab1, Muhammad Faran Tahir1 and Abuzar Hasnain2

... using three application methods (Preventive, Curative and after symptom appearance). Findings of contemporary study showed that, Azadirachta indica exhibited the best results in-vitro (10.29 mm) followed by Allium sativum (12.24 mm). Under greenhouse conditions, the combination of Azadirachta indica and Allium sativum expressed significant effect against BLS of rice with least disease incidence percentage (18.07%), when applied as preventive treatment. These ...
Suherman Suherman1*, Muhammad Fahri Rizky1, Zaki Bahrul Fikri1, Hadiyanto Hadiyanto1,  Zane Vincevica-Gaile2, Anwar Saeed Khan3, Erkata Yandri4,5 and Iswahyudi Iswahyudi6 
...dried using conventional methods. Although, traditional drying is relatively simple, it has disadvantages such as relatively long drying time, relatively low drying quality, significant damage, and the sun’s ultraviolet rays can also damage the chemical components of dried materials. Therefore, a new drying process technology, Photovoltaic Ventilation (Solar Dryer) was developed to overcome the above problems. In this research, onions were dried using a ...

Shoaib Zawar1, Muhammad Waqas Yonas1,2*, Muhammad Mujahid Akbar1 and Abeer Ahmad1

...and productivity, sowing methods may greatly influence crop performance. This study aimed to assess the effects of different sowing methods on spring wheat yield. The experiment was carried out in 2020-21 and repeated in 2021-22 under Randomized Complete Block Design (RCBD) with three replications focusing on five sowing methods (i.e. the conventional broadcasting, bed sowing (75 cm distan...

Anom Yusuf Tri Bambang Susilo1, Muhammad Fathin Hanif1, Bambang Ariyadi2, Ahmad Romadhoni Surya Putra3, Ali Agus1*

...s the use of statistical methods to discover patterns and trends in scientific publications. This approach includes studying the quantity of publications, identifying the countries with the highest productivity, and defining the main fields of research. Through this analysis, we could gain valuable understanding of the dynamics and limitations in knowledge within the field. In this case, a bibliometric analysis was conducted on the cage-free housing system in ...

Amal Ahmed Abdel-Halim1, Esteftah Mohamed El-Komy2, Ahmed Mosaad Abdelsalam2, Gomaa Said Ramadan2*

...way analysis of variance methods were applied to examine the differences in various parameters. The results revealed significant variations in the weekly mean body weight of the quail birds at weeks 0, 1, 2, 3, 4, 5, and 6 among the different hybrid lines. These differences imply varying growth rates and body weights throughout the study period. Furthermore, the average carcass characteristics displayed significant variation among the hybrid lines. It was obse...

Mahfuza Ferdous1, Sabuj Kanti Nath1 and Mustasim Famous2*

...on (p<0.005). Feeding methods by implementing new technological approaches, farmers can improve milk quality and gain more profit by minimizing feed costs. 
...

Muhammad Muneeb1, Ali Hussain2*, Qurat-ul-Ain Ahmad3, Arshad Javid1 and Jibran Hussain3

...ifferent physicochemical methods have been used to reduce these pollutants but all have their own limitations including production of secondary pollutants. The current study was designed to show the effect of cellulolytic and sulphate-reducing bacterial species in the form of a co-culture to treat an in-vitro prepared sulphate-rich wastewater while employing various agro-industrial organic waste as economical growth substrates. A combination of sulphate-reduci...

Alain Kouam Simo1, Roland Nankam Chimi1*, Stevine Ngoueni Megouo1, Ariane Megne Fossouo1, Félicité Flore Djuikwo-Teukeng2, Mamoudou Abdoulmoumini3

...September 2023.Screening methods by visual exploration of the mesenteric veins in search of adult worms and coproscopic analysis using the filtration technique were used. This last technique, although being the technique of choice because it is done on animals in ante-morten, gives less reliable results than macroscopic observation in post-mortem. Of the 700 animals explored, we had a total 50.6% of animals affected by schistosomiasis with prevalence’s o...
Olagbaju Olawole1, 2, Ayoola Mathew*1, Lawal Tunde1, Olorunleke Solomon3, Oladejo Opeyemi1, Oguntunji Abel1 , Alabi Olufemi1 , Aderemi Foluke1, Ajayi Abimbola1
...choosing synchronization methods based on specific breeding objectives. 
 
Keywords | Small ruminant, Reproduction, Estradiol, Progesterone, Estrus, Goat
...

Saba Mehnaz1*, Farhan Ahmad Atif2, Rao Zahid Abbas1, Muhammad Kasib Khan1 and Muhammad Saqib3

...d appropriate diagnostic methods, the study was carried out to detect Anaplasma marginale and Theileria annulata from buffaloes of Sheikhupura, Jhang, and Rawalpindi districts by using duplex PCR. A total of 1,152 blood samples of buffaloes were screened for epidemiological investigations. DNA extracted from blood samples was used for the amplification of the msp1β gene and cytochrome b gene by using single and duplex PCR. The prevalence of these pathogen...

Noor Rahmawati*, Dea Indriani Astuti and Pingkan Aditiwati

...) and spectrophotometric methods were used right away to assess the potential of endophytic fungus to create antioxidant chemicals. Additional investigation on the endophytic fungi that produce antioxidant chemicals was carried out using ITS molecular analysis. Thirty strains of endophytic fungus were taken from twigs and leaves. Based on the early TLC examination, a number of endophytic fungus showed the presence of compounds with antioxidant function. Endoph...

Zahraa M. Mahdi 

... use of characterisation methods such as SEM, XRD as well as FT-IR for analyzing and understanding the structural and functional properties of the nano-composite membranes. Concerns like the production of the fouling membrane and resistance troublesome conditions are dealt with, yielding a detailed view of the limits in this field. This paper is summarized with future applications of nanocomposite membranes in enhancing the processes of forward osmosis, wastew...

Kusuma Adhianto*, Tina Rahmawati, Rio Ramanda, Muhtarudin Muhtarudin, Sri Suharyati 

...alyzed using descriptive methods for pH, consistency, color, and odor, while volume, motility, live spermatozoa, abnormality, and concentration were analyzed using ANOVA and followed by Bonferroni post hoc test at a significance level of 5%. The findings of this study indicate that there is no significant (P>0.05) difference in pH, consistency, color, and odor between natural vitamin E and synthetic vitamin E supplemented groups, and they do not significant...

Hazrat Younas1,*, Khuram Nawaz Sadozai1, Amjad Ali1 and Rizwan Ahmad2

...tical and linear staking methods, with former incurring a production cost of Rs. 6,99,125/- compared to Rs. 1,84,000/- for the latter. Correspondingly, structured vertical staking led to higher profits, with Rs. 4,25,875/- compared to Rs. 1,92,000/- for linear staking, demonstrating the increased profitability of vertical staking practices. Based on the analysis, the study offers recommendations to foster the growth, promotion, and enhancement of vertically st...

Mohammed Abdulameer Rashid Al-Sarray1, Hasanain Jihad Neamah2*, Sarah Gatea Fayyadh AL-Omairi1

...ric acid reaction (TBAR) methods, however, 2, 2 – Diphenyl - 1- picrylhydrazyl (DPPH) was used to estimate the TAC of seminal plasma depending on the absorbance. The P1, P2, O1, and O2 groups recorded a highly significant (P≤0.01) decrease in seminal MDA level. The P1, P2, O1, and O2 groups recorded a highly significant (P≤0.01) decrease in seminal MDA level. On the other hand, the results suggested a higher TAC increase for all groups than the con...

Muhammad Farooq* and Sanam Zarif

...uminating their effects, methods of management, and related difficulties. The necessity to supply the demand for fuel wood, lumber, and animal feed has historically led to the introduction of exotic species into Pakistan. Even while invasive species are not as severe in Pakistan as they are in some other nations, their effects on the region’s biodiversity are frequently overlooked. Significant ecological ramifications result from the roughly 700 foreign ...

Nesreen Ahmed Nasser1, Anas H. Sadek2, Bayadir Abdulhussein Mahmeed3, Osama Akram Mohsein4* 

... are the main diagnostic methods. Aims of the study: Estimating the levels of prostate cancer antigen and leptin in patients with prostate cancer and their role in the development of the disease. Methodology; The study included 35 men suffering from prostate cancer and 35 healthy men as a control group. Samples were collected at Al-Kadhimiya Hospital for the period between 1/11/2023 and 1/3/2024, and 5 ml was withdrawn from each participant and placed in a gel...

Natalia Shchur1,2*, Tetiana Mazur1, Orest Katsaraba3, Ihor Halka2, Ludmila Shalimova2, Larisa Moskalenko4, Tetiana Ponomariova-Herasymiuk4, Maksym Lusta4, Vitalii Nedosekov1

...e the indication system, methods were modified by reducing cultivation time, simultaneously using selective nutrient media of various compositions, and employing both commercial materials and those prepared from components. Additionally, a comparative assessment of two isolation methods was conducted using a model of 138 samples of cecal contents from poultry (n=106) and animals (n=32) from farms in Vinnytsia, Volyn, Kyiv, a...

Saima Maher1*, Salma javed2*, Gul mina1, Rizwana Salam1, Farah Mukhttar1, Noureen Khan3 and Fakhra Batool3 

...op practical application methods for field use. The research emphasizes the need for further investigations to isolate active components, optimize concentrations, and develop practical application methods for field use. These findings contribute to ongoing efforts aimed at developing environmentally sustainable nematode management solutions.

...

Hina Yasin1,2*, Shaukat Mahmud2, Hina Abrar1, Kaneez Fatima3, Rabia Bushra1 and Saima Zahid2

...ard drug using different methods of evaluation for analgesic activity. Brine shrimp bioassay in different leaves extracts also showed similar effects likewise to the standard drug vincristine sulphate. All extracts exhibited marked cytotoxic activity as well against the Artemia salina (brine shrimp eggs). Based on the findings, it is concluded that the leaves extracts of the C. bonplandianus have potent analgesic and cytotoxic effects but in higher doses only....

Ashraf Samir Hakim1*, Doaa Diab Khalaf1, Engy Farahat1, Mohammed Darwish Mohammed1, Wahid Hussein El-Dabae1, Khaled Abd El-Hamid Abd El‑Razik2, Amany Nabil Dapgh3, Ehab Ali Fouad4, Hussein Ahmed Abuelhag1

...phenotypic and molecular methods. Besides that, the study determined their multidrug resistance patterns, particularly the existence of the gene responsible for carbapenem resistance. A cross-sectional study performed on one hundred and ninety two urine samples owned to urinary infected humans, dogs and cats were obtained from Egyptian laboratories in Great Cairo governorates. The samples were cultured onto the suitable media; the presumptive E. coli colonies ...

Eman Mamdouh Qenawy1, Mohamed Abou-Ellail1, Fatma Abdel-Motaal2, Mohammed O. Alshaharni3, Nady Khairy Elbarbary4*

...cterial analysis and PCR methods. The fraud samples exhibited a higher overall bacterial count than pure bovine meat. P. aeruginosa and E. coli were identified in 41.1% and 30% of the examined samples, with a high occurrence rate in minced beef samples. All P. aeruginosa isolates examined by PCR carried the rpoB gene, and 70% and 40% showed positive lasB and exoS virulence genes. Meanwhile, E. coli isolates showed hylA, stx1, and stx2 virulence genes in 50%, 8...

Jahid Hasan Tipu1, Md Ashraful Islam2, Md Altafur Rahman3, Md. Nazim Uddin4, Obaidul Islam5* 

...iable and cost-effective methods for predicting live body weight in Black Bengal goats. 

...

Preetysh Nanda Patnaik1, V.K. Venkataramani1, N. Jayakumar1, R. Durairaja1, Adyasha Sahu 1 and C. Sudhan2*

...e. A set of multivariate methods (Principal component analysis and Discriminant function analysis) was performed to make detailed investigation on distinction and pattern of morphological variations among all the four studied species, which revealed that there is significant variation in morphometrics of the portunid species. The first two components of the principal component analysis explained 99.99% of the overall variation. The first principal component (P...
Faiza Ghazanfar1, Masood Rabbani1*, Aamir Ghafoor2 and Muhammad Hassan Mushtaq3
... chain reaction) testing methods. Phylogenetic tree analysis was also performed for genetic identification of the species. in vitro antibiotic disc diffusion method of those selected isolates was performed against 20 most common human therapeutic antibiotics. The isolates were found to be highly resistant against most of the available antibiotics leaving behind very less choice of selection for treatment. The results are as follows: ciprofloxacin (91%), clinda...

Rahmat Ullah Khan* and Karim Gabol

...transect and point count methods. A total of eighty-three bird species were reported that belonged to 15 orders and 40 families; of these, the Passeriformes was the dominant order. However, the habitat having the richest average avian diversity was; agriculture at 51.42% (540.85±25) followed by residential at 42.61% (706.78±32) and low at mountains at 5.96% (165.87±36). Regarding feeding habits, most bird species were insectivores (49.39%)...

Saleem Abid1* and Muhammad Jahanzaib2

...ments. Various stability methods were used for stable genotypes identification across environments. According to different stability measures groundnut genotype 11AK011 turned out to be the most stable entry as revealed by Wricke’s ecovalence, mean square deviation, coefficient of determination, Shukla’s stability variance and coefficient of variation. The GGE biplot analysis also confirmed that 11AK011 groundnut genotype and can be considered as a...

Yasemin Öztürk

... count and line transect methods were applied between the early hours of the morning and sunset, when the birds were active. According to Shannon-Wiener diversity values, the diversity was determined to be highest in summer and lowest in spring. Comparisons made among sites according to bird species abundance determined that plots L24c3, L25d4, and L25d3 had the highest Shannon-Wiener diversity indices (H = 3.081; H = 2.77; H = 2.77, respectively). These sites...

Fazli Amin1*, Amjad Usman1, Maqsood Shah¹ and Sarzamin Khan²

... cost constraints, these methods may not always be feasible, and alternative nutritional strategies can be followed, including the use of balancing of nutrients. One such nutritional strategy for reducing stress is the incorporation of Tenebrio molitor (mealworm) as a feed additive. Therefore, this study was designed to investigate the impact of T. molitor as a feed supplement on the performance, immunity, and economics of heat-stressed broilers. A total of 15...
Nur Anis Hashim1, Nor Hasima Mahmod1*, Abubakar Abdullahi Lema2, Lee-Hoon Ho3 and Mohammad Moneruzzaman Khandaker1
...h calyx and three drying methods investigated, FC in ethanol produces the highest potential in antioxidant activity, followed by FD, AD and OD. Phytochemical screening tests found that the fresh and freeze-dried calyx possessed important antioxidative metabolites such as anthocyanins, tannins, saponins, glycosides, flavonoids and terpenoids. The findings demonstrated that fresh calyx retained the highest antioxidant capacity, thus providing insights into post-...

King Hei Ip

...mproving data collection methods and conducting further studies on bat immunity and viral co-infection would further the understanding on infection dynamics and control as well as the relationship between bats and viruses, allowing a better prevention of future pandemics through a more understanding towards the mechanisms and triggers to viral disease emergence. 

...

Muhammad Ehsan Maqbool1*, Muhammad Nadeem Mushtaq1, Tamathues2, Khadija Tul Kubra1, Arish Zulfiqar1, Muhammad Shoaib Sarwar1, Zeeshan Yousaf3, Mudassar Yaseen1, Farwa Nosheen3

...collected with different methods. Most species (9) belong to the order Hemiptera, four species from Lepidoptera, three species from Coleoptera, and one species each from Diptera, Orthoptera, and Thysanoptera. Amrasca biguttula biguttula (31.38%) was dominant species throughout the study period followed by Aphis gossypii (12.39%), Podagrica sp. (10.98%), Bemisia tabaci (9.45%), Syllepte derogate (8.30%), Mylabris pustulata (7%), Phenacoccus solenopsis (4.17%), ...

Seyed Ahmadreza Hashemi1, Mastooreh Doustdar2, Asadullah Ali Muhammad3* and Reza Abbaspour Naderi4

...ters. In this study, two methods were used to determine the biological reference points (BRPs) of Kawakawa in Iranian southern waters (the Persian Gulf and Oman Sea). Catch data were gathered for 26 years (1997–2022), and R software and the limited data technique were used to determine the optimal catch limit. During this time, the average catch (Ct) was 22439 tonnes (95% confidence interval 18299 -26638 tonnes), and it has climbed considerably over the ...

Budi Guntoro1*, Nguyen Hoang Qui1,2, Ahmad Romadhoni Surya Putra1, Nguyen Thi Anh Thu1,2 and Nguyen Van Vui2

...ne of the most effective methods to reduce environmental emissions in pig farming. By analyzing the extended technology acceptance model (TAM extended), this study investigated the factors of social profiles, production characteristics, and perception of farmers affecting the acceptance of biogas systems in pig farms. The data showed that occupation, perceived usefulness, information awareness, social influence, and perceived cost were significant predictors o...

Soumia Ed-Day1*, Meryam Laanaya1, Chaimae El Kourchi3, Radia Elgui1, Fatima Ezzahra Kacimi2, Latifa Didou1, Samira Boulbaroud2, Hicham Harhar3, Fatima-Zahra Azzaoui1

Mostafa R. Zaher1,2*, Amir A. Shehata1, Azza M. El Amir2, Naglaa M. Hagag1, Reham H. Tammam3**

Muhammad Umair Khan1*, Abdur Rehman1, Mansoor Ali Khan1, Abdur Rahman Khan1, Sajid Ali1 and Zeeshan Ahmad2

...rations. Several pulping methods can be used for non woody raw material such as soda/soda anthraquinone and organosolv. The availability and utilization of raw materials of non-wood for Pakistan’s pulp and paper sector is examined in this study. Non-wood fibers offer a variety of physicochemical characteristics that make them a viable raw material substitute for Pakistani pulp and paper manufacturers.

...

Wan Nurfarzana Wan Mohamad Zani1, Norrizah Jaafar Sidik1*, Asmah Awal2, Nurul Izzati Osman3, Lyena Watty Zuraine Ahmad1 and Mohd Khairi Nordin4

... Traditional propagation methods are inconvenient and time-intensive. As an alternative, micropropagation techniques are opted to overcome these challenges. This research aimed to develop a micropropagation protocol for expanding D. asper bamboo through the utilization of various propagule sizes and a comparative analysis of their growth on liquid and semi-solid MS media. In vitro nodal segments were initiated on MS media supplemented with 4 mg L-1 BAP and 0.5...

Karar Ali Abdulkhuder* and Abdul Kareem Salman Alyassari 

...y testing using standard methods. Additionally, a subset of isolates demonstrating high resistance was selected for molecular analysis to detect the presence of antibiotic resistance genes, including mecA, ermA, and ermB. The antibiotic susceptibility testing revealed varying degrees of effectiveness among the antibiotics tested. Notably, mecA, ermA, and ermB genes were detected in all samples analyzed, indicating widespread methicillin and macrolide resistanc...

Qasim Jawad Amer, Wissam Ahmed Sabah Al-Janabi* 

...e potential of molecular methods as a reliable alternative to traditional techniques for accurate and rapid diagnosis of T. evansi in camel populations. For this purpose, this study was designed to collect seventy-five blood samples (n=75) from camels of different ages of both sexes (male and female) from different areas of northern and southern Babylon.Each blood sample was placed in a special anticoagulant tube (EDTA), with a label containing the number, age...

Ye-Ling Lao, Jin-Long Huang, Cheng-He Sun, Xiao-Ying Huang, Ting Wu and Qun Zhang*

... likelihood and Bayesian methods and verified the classification of the target species M. altispinosus. According to our analysis, further studies should focus on the relationships between Amphilophus and Symphysodon. By determining the complete mitogenome of M. altispinosus, this study improves the phylogenetic resolution of New World cichlids. This new resource is highly important for the classification of New World cichlids and provides valuable data for su...

Shibao Guo1, Junhua Chen1, Nan Song2, Fangmei Zhang1*

...d and bayesian inference methods suggested the genus relationship of Tephritidae: ((Bactrocera, Dacus, Zeugodacus), Felderimyia, Anastrepha), (Acrotaeniostola, (Neoceratitis, Ceratitis), Euleia, Rivellia), (Procecidochares, (Tephritis, Sphaenisscus))))). Our results presented the first mitogenome from Sphaeniscus and provide insights into the species identification, taxonomy and phylogeny of S. atilius.

...

Pakistan Journal of Zoology

October

Pakistan J. Zool., Vol. 56, Iss. 5, pp. 2001-2500

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