Reviewed by David Svolba, Fitchburg State University, USA email: [email protected]
Science, Religion and Culture
...e should not be taken as expression of skepticism on Leiter’s part concerning the case for religious tolerance. Leiter affirms the strength of that case in the book’s opening chapter....
Sandeep Satapathy and Rupam Ghosh
...h IL-6 signalling is the expression acceleration of sequestrome 1(SQSTM1) / aggrosome and colocalization of HDAC6 to the cytoplasm. The key player HDAC6 is investigated for modelling several intracellular processes that come into action to bring out the viral clearance. The shuttling of HDAC6 takes place from nucleus to cytoplasm and vice versa controlling the overexpression of IFN-beta and ISGs which subsequently triggers ...
Qingzhong Yu
...sertion site for optimal expression of a foreign gene without compromising the replication of the Newcastle disease virus. Recently, inserting a green fluorescent protein (GFP) gene upstream from the GE sequences of the viral genes and subsequent quantitative measurements of the GFP fluorescence intensity, we have concluded that the P and M junction region is the optimal insertion site for a high level of foreign gene expression...
Rabbi Paul Shrell-Fox
...e. Some have found other expressions of their talents, while others anxiously await the opportunity to find alternate means of financial support. Specific issues of Jewish dietary practice, Sabbath observance and daily prayer are addressed, as well as an exploration of the rabbis’ connection to the Jewish people, despite waning practice....
Tauseef Taj Kiani1*, Mozammil Hussain1 and Hidayat ur Rahman2
... has its most signi cant expression in maize than other cereal crops. Exploitation of this phenom- enon of superior performance of F1 progeny over their parents not only trans gured maize breeding schemes but also constituted the foundation of maize seed industry. Evaluation of per se performance of parental lines and estimation of heterosis and inbreeding depression are prerequisites to start hybrid development program. In this context, we explored heterosis ...
Rongfei Lu1, 2, Zhiyang Liu1, Peng Fang1, Guangyi Chen1, Feng Sun1, Yongjian Fan1, Yijun Zhou1, Tong Zhou1*
...tandard for the relative expression of target genes, the selection of reference genes is crucial. This review describes the history of the RT-qPCR technology, emphasizes the importance of reference genes and enumerates several algorithms to screen reference genes to normalize the RT-qPCR data. Additionally, several possible improvements in the selection of reference genes are discussed....
Qaizar Ahmed1, Fida Mohammad2, Hidayat-ur-Rahman2, Sheraz Ahmed2* and Fakharuddin2
...tive gene actions in the expression of traits. Performance of hybrids was environment-specific. However, heterobeltiosis estimates ranged between -7.9 and 25.90 for leaf area, -6.76 and 130.8 for number of cured leaves kg-1, -10.8 and 17.1 for yield, -16.0 and 9.94 for grade index, -10.4 and 68.66 for nicotine and -26.89 and 31.25 for reducing sugar, respectively. The current study identified KHG24/Spt G 28; KHG21/NC606 and Spt G 126/KHG24 as best c...
Felwa A. Thagfan1, Mohamed A. Dkhil1,2* and Saleh Al-Quraishy1
...cted mice. Moreover, the expression of the goblet cell mucin gene, muc2 was upregulated after treatment with the root extract. Based on our results, it could be concluded that, S. persica root extracts acts as anticoccidial agent....
Laila M. Fadda1, Nouf M. Al-Rasheed1, Iman H. Hasan1, Hanaa M. Ali2,3*, Nawal M. Al-Rasheed1,4, Musaed Al-Fayez5, Aly M. Ahmed5, Nada Almutlaq1, Nehal Qasem1 and Reem Khalaf1
...l investigations for the expression of the proapoptotic protein (Bax) and the expression of macrosialin cluster of differentiation (CD68). APAP elevated of most of the previously measured parameters and decreased GSH, SOD, and total protein levels. Liver sections of H&E demonstrated liver injury characterized by centrilobular hepatocellular necrosis, CD68, and Bax expressions were also...
Characterization, Tissue-Specific and Developmental Stage Expression of Somatostatin in Coilia nasus
Siyu Yang1, Fukuan Du2 and Pao Xu1,2*
... which demonstrated high expression level in the brain. During embryogenesis, SS1 mRNA was detected during early-stage embryonic development, decreased during subsequent developmental stages then increased gradually from the stage of midgastrula onward. This study provides some basic evidence that SS1 may play a role in growth, development and metabolism in C. nasus, and provides a basis for further study of SS neuropeptide family in C. nasus....
Wenmin Cheng1,2*, Weirong Pan1,2, Yubo Qing1, Yingchao Liu1, Xingqin Zha1,2, Yan Huang2, Jige Xin1,2, Hongjiang Wei1 and Yangzhi Zeng2
...) by using a prokaryotic expression system and compared the difference on the developmental effects between exogenous Npm and its structural analog, polyglutamic acid (PGA). We chose the pMD18-T vector for Npm expression and transformed E. coli BL21 (DE3) cells to construct a prokaryotic expression system. The results showed that the recombinant Npm protein of 32 kDa could be obtained. Rec...
Ahmed Samy, Wesam Mady, Naglaa M. Haggag, Samah H. Mohamed, Ebtissam N. AlShamy, M.K. Hassan
...time PCR, cytokines gene expression were examined in response to infection with two strains of Egyptian H9N2 (namely V3 and RSF/1) in chicken peripheral blood mononuclear cells (PBMC). Hemagglutinin gene sequence analysis of the two strains reveal high similarity with difference close to cleavage site (HARSSR/ GLF) and (PARSSR/GLF) for V3 and RSF/1 respectively. Results of growth kinetics in PBMCs showed that V3 replicated more efficiently comparing to RSF/1 a...
Xiangxing Zhu1, Junyu Nie1, Shouneng Quan1,2, Huiyan Xu1, Xiaogan Yang1, Yangqing Lu1, Kehuan Lu1 and Shengsheng Lu1*
...ocyte-specific transgene expression of transgene have made significant contributions to understanding astrocytic structure and function in vitro and in vivo, evidence suggests that mouse models are limited and sometimes do not fully replicate the complete spectrum of neurological phenotypes seen in human diseases. On the contrary, pigs, especially mini-pigs, show exciting potential for modeling human CNS diseases due to similarities in body size, anatomy, life...
Muhammad Saeed* and Iftikhar Hussain Khalil
...hat non-additive genetic expression were pre-dominant for most of traits studied. Line BRF3 and testers ZM04 were good general combiners for most of traits under consideration, while F1 hybrids B4N11 × PS08, B4N11 × JZ, BRF1 × ZM04, BRF1 × KT10 were best specific combiners observed for several important traits including grain yield plant-1 which can be subsequently utilized in future wheat breeding to develop high yielding new wheat cul...
Zamarud Shah, Safdar Hussain Shah, Asad Jan and Gul Shad Ali
...confirmation, HsfA1d overexpression lines (OX1, OX2 and OX3) were evaluated for their response to heat stress. Overexpression lines on average showed 33.26% less electrolyte leakage after induction of heat stress at 42°C compared to wild type plants (WT). No significant decrease was recorded in the chlorophyll content of overexpression lines whereas significant decrease was recorded in...
Wan-Long Zhu1 and Guang Yang2,*
...amic neuropeptides genes expressions on body mass regulation under different photoperiods in Eothenomys miletus, body mass, food intake, serum leptin levels and hypothalamic neuropeptide neuropeptide Y (NPY), Agouti related peptide (AgRP), pro-opiomelanocortin (POMC), cocaine and amphetamine regulated transcript (CART) expressions were measured. The results showed that short photoperiod reduced body mass and body fat ...
Gabriela Mansano do Nascimento, Helena Lage Ferreira and Clarice Weis Arns
...already reported the overexpression of these genes against IBDV infection in susceptible cells. The protection ability of ISGs to safeguard chicken cells against the virus could be potentially applied for vaccine production, better vaccination protocols and infection control in the future....
Jiancheng Zhai, Li Gao, Yanling Xia and Heping Li*
... Ci2) presented a higher expression in antler mesenchyme of female reindeer, but the other two (Ae1, Af3) were the opposite. The results of Blast in GenBank showed that they shared high homologies with SMAD5 (95%), COL6A3 (95%), SLC17A5 (93%), TPM4 (99%) and HMGB1 (98%), respectively. Quantitative RT-PCR validation of gene COL6A3 showed that the expression in both sexes was consistent with the results of mRNA differential di...
Yang Wang1, Zhide Cheng2, Mengjie Tang3, Haixia Zhou1, Xiaolu Yuan4,Muhammad Aqeel Ashraf5, Shuting Mao1 and Jing Wang1,*
...ne. The mRNA and protein expression levels of Ldh-c in plateau pika skeletal muscles were determined by real-time PCR and Western blot. The LDH activities, lactate contents and ATP levels in skeletal muscle were compared between the experimental group and the control group. The results showed that 1) the expression levels of Ldh-c mRNA and protein were 0.804±0.059 and 0.979±0.176, respectively; 2)...
Jun Cui, Xiaoxu Zhou, Zhicheng Wang, Derong Kong, Xuemei Qiu, Hongdi Wang and Xiuli Wang*
...d in liver, with limited expression observed in intestine, skeletal muscle and kidney. CcWap65 was significantly up-regulated in the liver of crucian carp after heat stress, suggesting that increase in Wap65 gene may be related to high water temperature stress and play important roles in high water temperature environment of crucian carp.
...
Faiz Muhammad,1, 2,*, Zhang Zhi-Feng,1, Shao Ming-Yu1 and Muhammad Shafi3
...e analysis revealed that expression is in order of muscle, gill, lymphoid organ and hepatopancrease.
...
Andrea Rezic1, Ivica Boskovic2, Piera Lubinu3, Marina Piria1, Tihomir Florijancic2, Massimo Scandura3 and Nikica Sprem1*
...o obtain a more accurate expression of form and better differentiation between the sexes.
...
Wesam Hasan Mohamed Mady 1*, Bing Liu2, Dong Huang2, A. Arafa1, M.K. Hassan1, M.M. Aly1, Pucheng Chen2, Yongping Jiang2* and Hualan Chen2
...n promoter. The in vitro expression of recombinant plasmid DNA was performed by transfection of 293T (HEK) cell line. The HA protein was analyzed using SDS-PAGE followed by Western blot and immunofluorescence assays. The pCAGG-optiH9 vaccine efficacy was evaluated by intramuscular immunization of SPF chickens with different concentrations of plasmid DNA and the sera were collected weekly post vaccination for antibody detection by HI test. All immunized chicken...
Shams Ur Rehman, Muhammad Arif and Abdul Mateen
Soil-borne viruses in major potato growing areas of Pakistan
... characteristic symptoms expression, bait test, infectivity assays and double antibody sandwich-enzyme linked immunosorbent assay (DAS-ELISA). Both the viruses were prevalent in Hazara and Malakand divisions. The average symptoms based incidence in Hazara divi-sion for PMTV was 8.83% and TRV was 6.80% while in Malakand division the average symptoms based inci-dence for PMTV was 8.46% and TRV was 9.95%. DAS-ELISA based average incidence in Hazara of PMTV and TR...
Sadia Munir1a, Asif Nadeem1a*, Maryam Javed1, Masroor Ellahi Babar2, Tanveer Hussain2, Wasim Shehzad1, Rajput Zahid Iqbal3 and Sidra Manzoor1
...d regulating the hormone expression in animals. In current study, Pit1 gene was screened for polymorphic sites in Sahiwal cattle. Samples from Sahiwal cattle breeds were sequenced using six set of primers. A total 15 polymorphisms; 12 in intronic and 3 in exonic region, were identified. The sequences of the amplified Pit1 gene fragments were aligned with the help of BLAST for SNPs identification. This is a first report toward genetic screening of...
Baohua Chen1,2, Wenzhu Peng3, Jian Xu2, Jingyan Feng1,2, Chuanju Dong1,2 and Peng Xu2,3,*
...WGD) of common carp. The expression patterns of all GST genes were established in various tissues, including brain, heart, spleen, kidney, intestine, gill, liver, skin, blood and muscle of common carp. Expression profiles provided us more evidences to understand GST gene functions as well as their functional evolution post duplication. Overall, the whole set of GST genes provide essential genomic resources for future biochem...
Wenqiao Hui1, Jishun Tang1, Dejian Zhu1, Qian Ban2* and Sheng Chen1*
...hways which modulate the expression of hypothalamic genes involved in reproduction and metabolism. We found that leptin significantly upregulated the LEPR in the adipose tissue of goat at the puberty onset stage. Moreover, we observed that LEPR, JAK2, STAT3 (P<0.05), KISS1 (P<0.05) were also upregulated in the hypothalamic tissue, except NPY, which was downregulated. Taken together, it is, therefore, inferred that probably leptin secreted in the adipose ...
Mehwish Faheem1,*, Saba Khaliq2 and Khalid Parvez Lone2
Wesam Hasan Mohamed Mady1*, Bing Liu2, Dong Huang2, Abdel Satar Arafa1, Mohamed Khalifa Hassan1, Mona Mehrez Aly1, Pucheng Chen2, Yongping Jiang2* and Hualan Chen2
...ed into pCAGGS mammalian expression vector under the control of chicken β-actin promoter. The construct was transfected into 293T HEK (Human Embryonic Kidney) cell line for in vitro expression of the recombinant plasmid DNA. The confirmation of H5 protein expression was performed using SDS-PAGE followed by Western blotting and immunofluorescence assays. The humoral immune response was...
Yijin He1, Song Ma2, Bo Liu1,*, Ting Xue1, Qunlan Zhou1, Wu Jin1 and Kui Chen2,*
...studied the differential expression spectrum of tissues in combination with bioinformatics analysis so as to understand the possible functions in response to chemical sensation behavior of taste receptors. The receptors when compared with T. rubripes, there was 99.2% identity in two kinds ORF sequences, with only 7 bases different, and there were 5 amino acids different between speculated fusion proteins. Under bioinformatics analysis, the T2R1 g...
Mian Ahmad Raza*, Ghulam Hassan and Naqib Ullah Khan
...nism was involved in the expression for maturity trait, plant stature, spike length, spikelet spike-1 and grains spike-1. Negative intercept of regression line indicated the major role of over-dominance in the inheritance of plant height, spikelets spike-1while for grains spike-1 the positive intercept of regression line indicated predominance of partial dominance in inheritance.F1 cross combination FS × JNB were observed as good specific combiners for s...
Fang Wang, Yong-Fang Jia, Po Wang, Qi-Yan Du and Zhong-Jie Chang*
...LET for 15 days, and the expression profiles of seven gonad differentiation related genes (Cyp19A, Cyp19B, Arα, ERα, Dax, Foxl2, Dmrt1) and gonad histological changes were examined. The results showed that LET could inhibit oocyte growth in female and promote the development of testes in males. For the gonad differentiation related genes, the expression profiles of Cyp19A and Cyp19B show a pattern of increase in ...
Zaheer Abbas*, Shaukat Ali, Jalal-Ud-Din and Ghulam M. Ali*
...beta glucuronidase (GUS) expression with average frequency of 40.56% was noticed with three days of co-cultivation period while two days of co-cultivation period resulted 24.07% transient GUS expression. Addition of 100 mgL-1 L-cysteine in co-cultivation medium increased the transient transformation by 33.9% compared to control treatment. It has also been observed that cysteine enhanced the expressio...
Saba Manzoor1,*, Ali Raza Awan1, Abdul Wajid1, Sehrish Firyal1, Muhammad Tayyab1, Muhammad Mansha2, Asim Khalid Mahmood3, Abu Saeed Hashmi1 and Muhammad Wasim1,*
... ailment.
...
Rehana Shahida1, Tasnim Farasat1, Shagufta Naz1,* and Shahjahan2
... study aims to study the expression of TNF-α among impaired glucose tolerant (n=35), diabetic subjects (n-35) and age matched healthy control (n=35), and to identify the relationship of inflammatory cytokines with insulin resistance and the molecular mechanisms involved in the interaction of atherosclerosis and hyperglycemia within the context of chosen pro atherogenic parameters including plasma glucose level, serum levels of insulin, IL-6, and TNF-&alp...
Xue-yang Wang1, Shang-zhi Zhang1, Ming-hui Liu2, Dong Yu1, Yan Ma1, Dong-qiong Fei1, Hai-zhong Yu1 and Jia-ping Xu1,*
... mRNA showed highest expression in hemolymph, significantly high expression levels in 4th instar, moth, and egg, stable high expression levels in the early embryonic development, relatively high expression levels in the 1st day and 5th day of the pupa stage, and highest expression l...
Zhenyu Chang1, Hui Zhang1, Khalid Mehmood1,2, Mujeeb Ur Rehman1, Xiaodan Yuan1, Zhaoyang Li1, Fazul Nabi1, Xiaoxing Wu1, Xinxin Tian1, Xueting Liu1, Jingen Xu3 and Donghai Zhou1,*
...liferation and TFF3 mRNA expression were examined by 3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide (MTT) assays and real-time quantitative PCR, respectively. The results showed that rhTFF3 effectively prevented the cell injury induced by LPS (P<0.05). A low concentration of LPS (10 µg/mL) down-regulated the expression of TFF3 mRNA, whereas a high concentration of LPS (100 µg/mL) up-regu...
Farid S. Ataya,1,2,* Dalia Fouad,3,4 Ajamaluddin Malik,1 Nikolaos E. Labrou,5 Mohamed S. Daoud1,6 and Hesham M. Saeed7
..., we report the cloning, expression and characterization of the glutathione transferase isoenzyme P1-1 gene from Camelus dromedarius (CdGSTP1-1). The coding sequence was cloned using RT-PCR. Sequence analysis demonstrated significant differences between amino acid sequence of C. dromedarius and other mammalian GSTP1-1 enzymes. Phylogenetic relationship was studied with different organisms belonging to animal kingdom and revealed that Cd...
Jianping Li1, Qian Jiang2, Wei Chen2, Yumei Li3, Huaizhi Jiang4, Jinlong Huo5 and Qiaoling Zhang2*
...ve addressed the diverse expression of the KIT gene and its protein ingoats of different fur colors. The effect of KIT mutations on KIT protein expression was examined in white cashmere and black cashmere goats. A single A→G missense mutation in exon 13 differentiated cashmere goats with different colors. Only a histidine (H)→arginine (R) amino acid (AA) change was detected at KIT exon 1...
Jing Xin Mao1,2, Guo Wei Wang2, Yuan She Huang3, Rui Wang2, Guo Ze Wang4, Bing Zeng1 and Fu Yuan Zuo1,*
...rative study on BPI gene expression in various tissues between Rongchang pig and Landrace, we confirmed the expression of pig BPI gene in various tissues to determine the difference expression of antibacterial related gene among indigenous breed (Rongchang pig) and artificially cultivated breed (Landrace), The Rongchang pig (0 day old, 28 day old, 120 day old) and Landrace BPI (0 day old, ...
Pei-feng Yin1,2,Xiu-xiu Li1,Qi-wei Zeng3, Cheng-chen Shen1, Le Gao1 andJian-zhang Ton2,*
...esponsive proteins which expression level more than 1.5-fold were identified, where 50 proteins were up-regulated and 28 proteins were down-regulated; The identified proteins were categorized into 16 classes, which are mainly including energy metabolism, gene expression regulation, oxidation-reduction reaction, cellular component and stress responses, and the stress proteins including Mn-superoxide dismutase and thaumatin-li...
Lihong Qin1, Guoliang Zhang1, Chaojie Zhu1, Jian Wu1, Zhihui Zhao2,* and Yumin Zhao1,*
...> and FKBP1B mRNA expression levels in different bull testes tissues (1-day, 12-months-old and 24-months-old). In addition, genes mRNA and protein levels were detected after bull testicular Sertoli cells were transfected with miR-122 and miR-449a. Meanwhile, DUSP4, PDK4 and FKBP1B overexpression experiments were conducted. Eventually, MTT assay was performed to observe the cells proliferation. The...
Bian Xunguang1,2, Li Jiancheng1, Xu Chu1 and Yang Li2,3,4,*
...ieve. In this paper, the expression of AR mRNA in various parts of the oviduct was analyzed by qPCR. Oviductal structure was observed by H&E staining. The distribution of AR in the cells was detected by immunohistochemistry. The results showed that the expression of AR mRNA was found in the infundibulum, magnum, isthmus, uterus and vagina of the oviduct. The expression of the AR mRNA i...
Malak Atiq Ullah Khan* and Fida Mohammad
...fect than G and E on the expression of phenotype for all traits. Average over nine environments, genotype G-79 exhibited maximum values for tillers m-2 (181 tillers); grains spike-1 (71 grains); grain yield (4862 kg ha-1) and thus appeared as leading genotype for yield and production traits. Similarly, G-79 had higher grain yield in all test environments except E-03 i.e. E-01 (4537 kg ha-1); E-02 (4840 kg ha-1); E-04 (5035 kg ha-1); E-05 (4976 kg ha-1); E-06 (...
RuiHua Zhang, YongFang Jia, TingTing Liang, QianWen Yang, QiYan Du and ZhongJie Chang*
...ng nervous system. Their expression has been seen in neural stem cells as well as differentiating neurons. However, despite their importance in development, a relatively low number have been characterized for freshwater fish. In this study, we were able to gain three full-length SoxD sequences from transcriptome sequencing data of carp: CcSox5, CcSox6 and CcSox13 (Cyprinus carpio SoxD).These genes were verified by sequ...
Tania A. Shakoori1,*, Soumble Zulfiqar2, Khadija Irfan Khawaja3 and Faisal Masud4
...ted. We compared genetic expression of Sirt1, AMPK, Akt, and mTOR in adipose tissue from mice treated with NaB versus placebo. Four groups of mice (n=27) mice were administered oral NaB, intraperitoneal (i.p) NaB, oral placebo or i.p placebo for 16 days. RNA was isolated from the epididymal fat pads and relative genetic expression of AMPK, Sirt1, mTOR and Akt was measured using real time PCR. Oral NaB group showed decreased ...
Yanjie Guo1, Weini Wu1, Hongmei Wang2, Xiao Guo2 and Yongping Xu2,*
...ridization to detect the expression and distribution of IL-18Rα in the inferior mesenteric ganglion of the female goat. Our results showed that IL-18Rα mRNA and proteins are expressed in the IMG, which provides molecular evidence for the study of immune and neuro-modulation in the female reproductive system.
... 
Sadaf Ghulam Rasul1*, Uzma Yaqub2, Maida Manzoor1 and Hafsa Mubasshar1
...ntial for aromatase gene expression that leads to aromatase and estrogen production. Danazol is a synthetic androgen whereas Letrozole is aromatase inhibitor. This study was conducted to compare the mean decrease in visual analogue scale (VAS) pain score with Danazol versus Letrozole for the pain management of endometriosis in females. A total of 140 females fulfilling inclusion criteria were registered and an non-probability purposive technique of sampling wa...
Hidayatullah, T. Mahmood, M. Farooq, M. A. Khokhar and S.I. Hussain*
* Corresponding author: [email protected]
PLANT GROWTH REGULATORS AFFECTING SEX EXPRESSION OF BOTTLE GOURD (LAGENARIA SICERARIA MOLINA) PLANTS
...00 µmol l ) on sex expression of bottle gourd (Lagenaria siceraria Molina) plants cv. Faisalabad Round was investigated under field conditions in National Agricultural Research Centre, Islamabad. Plants sprayed with distilled water were considered as control. Among all foliar agents, the response of -l GA3 3 and MH was found better. Exogenous application with 30 µmol l GA maximally increased the pistillate flower production as compared to control. ...
Tasawar Sultana*, Farah Deeba* and S.M. Saqlan Naqvi*
HIGH-THROUGHPUT AGROBACTERIUM-MEDIATED TRANSFORMATION OF MEDICAGO TRUNCATULA IN COMPARISON TO TWO EXPRESSION VECTORS
...vector was compared with expression vector from pCAMBIA series over-expressing same gene (pCOsRGLP1). A lower number of explants generated hygromycin resistant plantlet for instance, 18.3 with pGOsRGLP1 vector as compared to 35.5% with pCOsRGLP1 vector. Transformation efficiency of PCR positive plants generated was 9.4% for pGOsRGLP1 while 21.6% for pCOsRGLP1. Furthermore 24.4% of explants -1 generated antibiotic resistant plantlet on 20 mgl of hygromycin whic...
Yanling Xia1,2, Heping Li2, Yuntao Liang2, Jichen Zhao2, Binshan Lu2 and Di Liu1,*
...6A gene had a higher expression level at day 90 than those obtained at day 60 and 30.
... 
