Xingdong Yang and Lijuan Yuan
... particularly a suitable animal species for the modeling of human enteric pathogen infections, immune responses and for vaccine evaluations. These are the basis for the successful establishment of the infection and disease models for human rotavirus, human norovirus and human enterovirus 71 using neonatal gnotobiotic pigs. The differences between germ-free and normal animals in the maturation status of immune systems caused ...
Ahmed Sultan Jatoi1*, Shahid Mehmood2, Jibran Hussain2, Hafiz Muhammad Ishaq2, Yassar Abbas3, Muhammad Akram2
Rodolfo Villagra-Blanco1*, Gaby Dolz1, Danilo Montero-Caballero2, Juan Jose Romero-Zuniga2
...rds from Costa Rica, and animals seem not show clinical signs. We recommend carrying out further studies, to determine the presence of BTV in goats and wild ruminants, and to identify serotypes present in the country....
Shumaila Manzoor1, Afshan Ahmed1 and Muhammad Abubakar2*
...rn of antibody titers in animals of different age groups was observed as less than 1 year, 1-2 years and more than 2 years for both ELISA and SNT. The peak immune response measured by SNT was 2.8log10 whereas ELISA detected serum antibody titer up to 2.4 log10. Findings showed that highest titers were seen in animals of age > 2 years i.e. 1.70 log10 (for serotype Asia 1), 1....
Muhammad Arif Zafar1, Murtaz-ul-Hasan1*, Aayesha Riaz1, Asim Shamim2, Muhammad Farooq Iqbal1 and Arfan Yousaf
E-mail | murtazhassan@yahoo.com
...sions. To this end, nine animals were selected clinical and sporadic papillomas different body parts. These animals were divided into three groups; Group A animals were operated for partial excision of the lesions, Group B were inoculated with autogenous vaccine alone and Group C were treated with inoculation of autogenous vaccine supplemented with immune-modulators (Levamisole a...
Muhammad Arif Zafar1*, Arfan Yousaf1, Murtaz-ul-Hasan1, Faisal Ayub Kiayani1, Matee ul Islam2 and Muhammad Farooq Iqbal1
Jonas Johansson Wensman1*a, Karl-Johan Leuchowius2,3 a, Jiting Yan1, Anna-Lena Berg4, Liv Bode5, Hanns Ludwig5, Sandor Belak6, Ulf Landegren2, Ola Soderberg2, Mikael Berg6
... wide range of mammalian animals, possibly including humans. Viral persistence in the brain has been frequently observed, however, the exact mechanisms behind BDV’s ability to establish persistence despite a prominent immune response are not known. Here we have used in situ proximity ligation assay (in situ PLA), a selective tool for studying virus-host protein-protein interactions. BDV P (phosphoprotein) and N (nucleoprotein) have previously been report...
Behzad Hussain1, 2, Abdullah Iqbal2, Muhammad Abubakar2*
...th blood of the infected animal. The occurrence of CCHF outbreak in Pakistan is increased quite significantly near Eid-ul-Adha. Haemorrhages are the major signs of this fatal disease. No treatment of CCHF is available currently but Ribavirin antiviral drug is used to cure this disease. There is currently no commercially available vaccine for this disease. It is recommended that the disease can be controlled by adopting preventive measures such as avoiding of t...
Haq Aman Ullah1*, Aneela Zameer Durrani2, Muhammad Ijaz2 and Aqeel Javeed3
...hly toxic for humans and animals. In the present study contamination status of aflatoxin B1 in cotton seed cake, wanda, wheat bran and homemade concentrate mixture of dairy goats was investigated in district Lahore of Pakistan. A total of Twenty 20 goat farms were randomly selected and out of that 40 feed samples 2 from each farm were collected in the month of April. All the samples were analysed for the determination of aflatoxin B1 by using reverse phase Hig...
Moustafa Kardjadj
...contagious transboundary animal disease and one of the most economically devastating diseases that affect cloven-hoofed livestock including cattle, goats, sheep, pigs and wild ungulates. Wide prevalence of the disease in Asia and Africa associated with huge economic losses to livestock farming and industry has increased global concern for the disease. Currently, 3 serotypes of FMD virus (O, A and SAT-2) and 06 lineage are circula...
Ashley C. Banyard1* and Satya Parida2
...aïve populations of animals have demonstrated an expansion of the distribution of the disease. Having been in the shadow of rinderpest virus for decades little is understood regarding factors that influence the outcome of infection with both viral and host genetics being thought to influence the outcome of disease. Following the successful global eradication of rinderpest virus, peste des petits ruminants virus has also been targeted for globa...
Moustafa Kardjadj1, Pam Dachung Luka2
...uncontrolled movement of animals across porous borders, limited financial capacity of farmers to expand or improve the systems, and inadequate supply of quality vaccines and the problem of delivery to maintain cold chain, there is need for a very strong regional coordination for effective and sustainable control of PPR in Africa. The African continent is currently divided into 57 countries (or independent territories), many of which rely heavily on livestock f...
Ahmed Zein Mahmoud1, Muaz Abdellatif 2, 3*, Luai Shazali1
...c-ELISA. Out of examined animals, 83 (62.9%) goats and 70 (33.2%) sheep were detected positive against PPRV antibodies, whereas camels appeared to be seronegative. Based upon the seasonal variations in the antibodies detection, environment appeared to be a significant factor on the level of antibodies in the tested small ruminants population. Taken together, results indicate the seropositivity of PPRV in the region and warrant future large-scale surveillance s...
Amitha Reena Gomes1, Belamaranahally Muniveerappa Veeregowda2, Sonnahallipura Munivenkatappa Byregowda1, Vinayagamurthy Balamurugan3*
... infected and vaccinated animals. With the advent of recombinant DNA technology, recombinant protein based vaccines and/or diagnostics are being tested in various heterologous systems across the globe for development of vaccines and/or diagnostic antigens. The recombinant viral proteins, virus like particle based vaccines, bivalent/multivalent vaccines, recombinant viral vectored vaccines, RNA interference as a therapy, suicidal DNAs, synthetic epitopes and pe...
Muhammad Abubakar1*, Shumaila Manzoor2, Jonas Johansson Wensman3, Emeli Torsson3, Qurban Ali1 and Muhammad Munir4
... the introduction of new animals in the herd, was investigated in an unvaccinated mixed herd of sheep and goats. Goats in the herd showed characteristic signs of PPR including nasal and ocular discharges, high temperature, diarrhea and ulcerative lesions in the oral cavity. A total of eighteen goats from a herd of sixty, were affected and two goats succumbed within two weeks. Interestingly, the disease was exclusively observed in goats and all sheep kept in th...
Zafar Khan, Asad Sultan, Rajwali Khan, Sarzamin Khan, Imranullah and Kamran Farid
... on the health status of animals. Chicken eggs are one of the main sources of protein but if contaminated by toxic heavy metals will cause a harmful effect on human health. The concentration of heavy metals (Pb, Cd, Cr, Fe, Mn and Zn) present in poultry egg and meat were determined in the following three districts; Peshawar, Dir Lower and Malakand of Khyber Pakhtunkhwa by using atomic absorption spectrophotometer. In all the three districts the egg albumen was...
Inamullah Khan, Muhammad Subhan Qureshi, Rajwali Khan, Syed Muhammad Sohail, Ijaz Ahmad, Muhammad Shoaib and Asim Ijaz
...ucellosis is an emerging animal’s as well as human health issue and holds an important position under the Biorisk Management framework and One Health perspective. A total of 410 and 202 blood samples were collected from cattle and humans respectively. Total 40 samples of subsistence farms and 30 nomadic herds were analysed. Forty eight farm workers, 40 farm owner 36 abattoirs workers, 15 veterinary assistants, 15 veterinarian and 40 butchers were also sc...
Arif Khan, Abdur Rahman, Shoail Akhtar, Siraj Uddin, Zahid Ullah and Nazir Ahmad
... The effect of breed and animal age on marbling, water holding capacity (WHC) and tenderness and sarcomere lenth of the two buffalo breed beef samples were investigated. A two x three factorial design was used to analyze the beef quality characteristic data in Genstat software. Breed and animal age had significant and their interaction has significant (P < 0.05) effects on marbling. Animal<...
Jannatul Noor, Md. Ahaduzzaman, Mir Md. Afzal Hossain, Mohammad Alamgir Hossain, Sardar Abdur Rahim and Md. Samun Sarker
...an another coat coloured animals (P=0.00). Age less than 6 months goats were mostly affected (55.0%) than adult goats (26.67%) (P=0.00). In this study, males were more prone (58.33%) than female goats (41.67%) (P=0.036). The ticks were more in goat reared under semi intensive system (63.33%) than free range (20.0%) and the intensive system (16.67%) (P=0.00). Ticks were found mostly around the ear (66.67%) than the other body parts. The most abundant ticks were...
Muhammad Ibrahim, Jawaria Ali Khan, Muhammad Sarwar Khan, Wasim Shehzad, Muhammad Avais, Ali Husnain, Naimatullah Khan, Muhammad Kamran Ameen and Abdullah Iqbal
...ion of healthy cows with animals under trial. Our study concluded that Vitamin E+ Se and Garlic were the most efficient drugs as 66% cows suffering from subclinical mastitis were recovered by treatment of each of them. Cows suffering from subclinical mastitis showed a 50 % recovery percentage when treated with lemon for five days and 33 percent cows recovered from subclinical mastitis when treated with Black Pepper for five days. Udder health status and milk q...
Wan-Long Zhu* and Gao Wenrong
... play important roles in animals’ physiological adjustments. In order to determine the contributions of temperature and photoperiod to seasonal changes in body mass and thermogenesis in Apodemus chevrieri, we examined seasonal-acclimatized and lab-acclimated animals on several physiological, hormonal, and biochemical markers indicative of thermogenic capacity. The results showed that A. chevrieri ada...
John Gachohi, Simon Karanja, Salome Wanyoike, Nduhiu Gitahi, Salome Bukachi, Kenneth Ngure
...-based biotic (human and animal) and abiotic (soil, water and weather) habitats in Leptospira propagation. Influential habitats would serve as key targets for interventions. To achieve this aim, we have designed a pseudo-longitudinal survey to be undertaken for two years in Kibera slums in Nairobi city Kenya. Human and animal blood and urine samples will be collected at the household level whereas soil and water samples will...
Ecevit Eyduran1, Daniel Zaborski2*, Abdul Waheed3, Senol Celik4, Koksal Karadas5 and Wilhelm Grzesiak2
...required feed amount for animals. The ascertainment of the body measurements associated with BW using data mining algorithms can be considered as an indirect selection criterion for future goat breeding studies. ...
Jose M. Rojas, Noemi Sevilla and Veronica Martin
Bibi Tayyaba Batool1, Aemal Tareen1, Shahjahan Shabir Ahmed1, Hira Ejaz1, Mohammad Azam Kakar1, Syed Abdul Rehman2, Mohammed Saeed1, Zafar Ahmad3, Mohammad Masood Tariq3, Mohammad Arif Awan3 and Muhammad Naeem Shahwani1*
Abdul Sattar Baloch1*, Abdul Rasheed1,2, Rahmatullah Rind1, Jam Kashif Sahito1, Rehana Buriro1, Muhammad Faisal Ayoob1 and Parkash Dewani1
... affects a wide range of animal species including human beings and is more severe in human than in animals. It imposes a huge burden on human health due to its zoonotic nature. Our study involves the testing of 100 sera samples and 50 milk samples from camels of three districts of Sindh province of Pakistan. Rose Bengal Plate Test (RBPT), serum agglutination test (SAT) and competitive-ELISA (c-ELISA) were used as screening t...
Abdul Mannan Babar1* and Abdul Hannan Nagi2
...in humankind, and not in animals. Thus far no practicable animal model of Keloid has been made. Recently a prototype rabbit ear keloid model has been produced by the authors by giving Transforming Growth Factor Beta 1 injection, and then skin punch excision on rabbit ear. In the present follow-up study consisting of eight refind techniques, best Keloid was produced by the technique where Transforming Growth Factor Beta 1 in...
Rongrong Li1, Kun Li2, Xiaoqiang Wang2, Houqiang Luo2, Gang Qiu2, Hui zhang2, Yanfang Lan2 and Jiakui Li2,3*
...hreat to human and other animals in this unique region of the world....
Hussain Ahmad1, Inamullah1, Ijaz Ali2, Tauseef Ahmad3, Muhammad Tufail4, Kabir Ahmad5 and Bibi Nazia Murtaza3,6*
...isease affecting several animal species and human beings. It is caused by Brucella abortus in animals and Brucella melitensis in human. The current study aimed to find out the prevalence of Brucella and active brucellosis in the human population of district Swat. Total 300 individuals (both female and male of all ages, meeting our inclusion/exclusion criteria) with informed consent were included in the study. Blood samples w...
KM Fakhrul-Islam, Mohammad Shah Jalal, Sonnet Poddr, Md Nurul Quader, Md Sahidur-Rahman, Avijit Dutta and Shuvo Mazumder
..., sheep, goats, and wild animals around the globe. This highly contagious disease causes severe economic loss due to reduced productivity of the affected animals as well as increased mortality in calves and kids. In this study proportionate prevalence of FMD in cattle was estimated and the distribution of FMD according to different factors was evaluated. Both retrospective and prospective FMD clinical cases were included con...
Asmaa Hamid1,* and Samia Kalsoom2
...ol of these experimental animals. Bengal gram however, proved to have a significantly (p<0.05) greater effect on lowering of these parameters....
Jixue Hou1,2, Xueling Chen3, Jing Wang4, Hongwei Zhang2, Yu Xi2, Jie Xia2, Xinyu Peng2,* and Xiangwei Wu2,5*
...is just limited to large animals, like pigs, rabbits, and big rats. The mice with reproduction fast cycle, lower costing, and close to human genes, can be showed about some phenomenon quickly, also more easy to form model of atherosclerosis. Thus, a mouse model of vascular injury is vitally important for researching the pathophysiological mechanisms to restenosis after percutaneous transluminal coronary angioplasty (PTCA) as well as translational approaches. I...
Aiza Tahir, Muhammad Asif, Zaigham Abbas and Shafiq ur Rehman*
...k products from mastitic animals are at high risk of foodborne infections. Escalating antibiotic resistance strived us to explore the alternate option to control mastitis. In ongoing era, bacteriophages are an alternate possible effective remedy. Therefore, in this study the infection ability of three lytic phages SA, SANF and SA2 was determined against ten isolates of Staphylococci and one Micrococcus, isolated from five raw milk samples. Their morphological,...
Yusuf Koc1, Ecevit Eyduran1* and Omer Akbulut2
...n the data set regarding animal science. To achieve the aim, 1884 Mengali lambs were provided for predicting weaning weight from sex (male and female), birth type (single and twin), birth year (2005, 2006, 2007, 2008 and 2009), farm (Research station, Mastung, Quetta, and Noshki), birth weight, dam age, and dam weight. To choice the best regression tree method, goodness of fit criteria such as coefficient of determination (R2%), adjusted coefficient...
Hafiz Aftab Ahmed1, Asif Ali2, Akbar Ali2, Salman Ahmed Abid3 and Sajid Umar4*
...hind limb extension. The animals were unable to flex the limb during walk. Diagnosis was based on case history, clinical sign and clinical examination. Upward fixation of patella was diagnosed in all three animals. After careful palpation of the ligament, medial patellar desmotomy was performed in lateral recumbence, under local anesthesia achieved by 2% lignocaine hydrochloride. The skin was apposed with simple interrupted ...
Guang-Xin E1, Yong-Ju Zhao1, Zhong-Yuan Zhang1, Yan-Guo Han1, Wei-Wei Ni1, Ming-Xing Chu2, Yue-Hui Ma2, Jian-Ning He3, Huai-Zhi Jiang4, Jia-Hua Zhang1, Zhong-Quan Zhao1, Yan Zeng1, Ri-Su Na1, Gao-Fu Wang5, Hang-Xing Ren5 and Yong-Fu Huang1*
...of economically domestic animals. Some microsatellites related to the fecundity of domestic animals have been identified. In the current study, we investigated five fecundity related microsatellites, including BM1329, BM143, LSCV043, OarHH35, and OarHH55, which had been identified in sheep. We estimated their diversity and population structure in three high fecundity goats (Jianzhou big ear goat, Jining grey goat, Dazu black...
Kiran Afshan1*, Sarwat Jahan1 and Mazhar Qayyum2
...e diagnostic efficacy of animal fascioliasis caused by Fasciola hepatica and Fasciola gigantica in Pakistan, we first time evaluated the diagnostic accuracy of commercially available bovine F. hepatica enzyme-linked immunosorbent assay (ELISA) for the detection of IgG against fascioliasis in sera of small ruminants. The result of current study indicated diagnostic accuracy of test 95.6%, while sensitivity and specificity of this assay for ...
Syed Fazal Baqi Kakakhel*, Asad Ullah and Abdur Rashid
...ial valley. Among the 84 animals found in Kalam 26 were females, 23 males of unidentified horn size, 12 young of six months and four yearling males of 18 months age; the number of males with more than 36// horn size was five, 18// to 36// and 18// horn size, seven each. While among the 52 animals observed in Mankial 14 were female, 15 males of unidentified horn size, five young of ...
Sumra Ashraf1, Zain ul Abdin1,*, Saqi Kosar Abbas2,Rao Sohail Ahmad Khan3, Muhammad Tahir1, Sehrish Rasool1, Maryam Anwar1 and Fiaz Hussain1
...e commonly obtained from animal secretions or plant exudates which include honeydew, fruit juices and both floral and extra-floral nectar. A direct behavioral assay was conducted to investigate the dietary preference and effects of different diets on the fecundity, sex ratio and longevity of B. hebetor. Three different diets [Honey syrup, sugar syrup, date syrup and a control (water)] were used with different solution percentage (25, 50, 75 and 90%). It...
Muhammad Luqman Sohail1,*, Muhammad Sarwar Khan1, Muhammad Ijaz1, Muhammad Avais1, Muhammad Yasir Zahoor2, Omer Naseer1 and Muhammad Usman Saleem3
...ffecting a wide range of animal species and humans. 18 Leptospira positive horses based on ELISA and 9 healthy horses were included in the study. Blood profile, serum biochemistry and mineral profile were analyzed at day 0, 7 and 21 of infection. Results showed significant decrease (P<0.05) in red blood cells (RBC), packed cell volume (PCV), hemoglobin (Hb), mean corpuscular hemoglobin concentration (MCHC) and platelets while total leucocytes count (...
Ali Zurwan1,*, Muhammad Moaeen-ud-Din2, Ghulam Bilal2, Zia-ur-Rehman1,2 and Muhammad Sajjad Khan1
...ty and random factors of animal. Indices values for Wood, Johnson and Hansen, Mahadevan, Ludwick and Petersen and Gajbhiye and Tripathi were 138.66, 75.68, 243.65, 85.84 and 275.41, respectively. Moreover, our results indicated that all indices were significantly affected by period and season of calving and lactation number except that Wood index that did not vary with seasons. Heritability estimates of various indices were low and ranged from 0.004 to 0.067 i...
Sadaf Ijaz Malik1, Kiran Afshan2* and Mazhar Qayyum1
...lood samples of the same animals were analysed. The infection was found prevalent throughout the year but during post monsoon season it was relatively high. Morphological identification of G. expalanatum was carried out on the basis of size and shape of worm. The hematological and bile biochemical changes were found in two groups. Pathologically significant changes were observed in the infected tissue. The parasites had formed pocked in the bile duct mu...
Mingqin Shao1*, Bin Chen1, Peng Cui2, Binbin Zeng1 and Jianhong Jiang1
...rmation for the study of animal behavior. The present study analyzed the daily travel distance, home range and group characteristics of scaly-sided merganser Mergus squamatus in Yihuang and Wuyuan counties, Jiangxi Province, China, from December 2012 to March 2013 and December 2015 to March 2016. Results showed that the daily travel distance of scaly-sided merganser was 3100 ± 1313 m; the daily home range was 122,460 ± 42,019 m2...
Akbar Ali1,*, Khalil Mohamed2 and Fawzia Toulah3
...fections of warm-blooded animals including man. In most adults it does not cause serious illness, but it can cause serious health problems in infants, pregnant women and immune compromised patients. Abortion is one of the most serious outcomes in pregnant women. In this cross-sectional study, a total of 162 women of child bearing age were included from Rafha city of Saudi Arabia. Demographic data was recorded. Specific Toxoplasma gondii IgG and IgM were...
Tuli Dey, Sonnet Poddar and Mukti Barua
... as the condition of the animal were followed up and gave the result of successful recovery without any complication....
Eman M. Farghaly1, Ahmed Samy1,2*, Heba Roshdy
... to cover the deficit in animal protein in developing countries including Egypt. However little is known about the prevalence and antibiotic resistance of major bacterial pathogens such as Escherichia Coli, Staphylococcus aureus Salmonella and Pasteurella spp. in Egyptian quail farms. Such information is important for drug choice and success of treatment as well as spotting the light on emerging antimicrobial resistance that represent major concern for public ...
Zahir Shah, Ahmed Nawaz Tunio, Shakoor Ahmad, Ijaz Ahmad, Javid Ali, Sher Bhader Khan
Shahzad Ali1,*, Heinrich Neubauer2, Falk Melzer2, Iahtasham Khan1, Shamim Akhter3,Tariq Jamil2 and Sajid Umar3
...de zoonotic infection in animals including Pakistan. A study was conducted to identify the potential cause of infection in three different cattle farms located at Potohar Plateau in Punjab, Pakistan. A total of399 serum samples from indeginous and exotic cattle were tested for brucellosis by RBPT, SAT and multiplex real-time PCR for Brucella sp., Brucella abortus and Brucella melitensis. A total of 20 (5.01%) samples were found seropositiv...
Tausif Ahmad1, Iahtasham Khan2, Saddaf Razzaq1, Saeed-ul-Hassan Khan1,* and Raheela Akhtar3
...il Veterinary Hospitals, animal markets and peri-urban livestock holdings in Rawalpindi and Islamabad. Serum samples were initially screened by the Rose Bengal Plate Test (RBPT). RBPT positive samples were subjected to a B. abortus specific indirect enzyme-linked immunosorbent assay (i-ELISA). Serum samples that were confirmed to be positive for B. abortus through serology were subjected to an rPCR in order to test its efficacy in detecting Br...
Adel Eid Mohamed Mahmoud*
... kg; in three groups (17 animals, each). Experimental rations were composed of concentrate and clover hay in a 60:40 concentrate: roughage ratio.There was no effect of bakery by-products inclusion on nutrients digestibility and nutritive values except digestion coefficients of crude protein and digestible crude protein. Ruminal pH values and ammonia nitrogen concentrations did not show any significant differences among groups.No significant differences were ob...
Yi-qiang Ouyang1, Zhen-xin Liang1, Shi-wen Huang2, Ying Zhang3, Shao-shi Luo1, Jin-ning Liang1, Jia-fu Li1, Yu He4 and Song-chao Guo1,*
...gn: justify;">Most small-animal studies of Alzheimer’s disease (AD) involve rats and mice, yet the tree shrew (Tupaia belangeri) is in many respects more similar to primates than rodents are. To demonstrate the feasibility and usefulness of using the tree shrew as an AD model, we examined how well the model recapitulates features of the disease. Injecting animals intraperitoneally with d-galactose to induce acut...
Wazha Mugabe1,2, Lawrence Akanyang1, Mackenzie Nsinamwa1, Batanani Moatswi1, Naledi Matthews1, Kealeboga Dipheko1, Imtiaz Ahmed Ujjan3 and Assar Ali Shah2*
...ium point for optimizing animal productivity, with limited range degradation. Therefore, the current study was aimed at determining and comparing fodder tree species composition along grazing gradient in fenced and unfenced grazing area in the Gaborone North Region. For each study area, two parallel transects measuring 1000 m radiating from the water points (borehole) were established. Sampling plots of sizes 10m x 10m were systematically placed along each tra...
Jakub Jan Skorupski
.... The study involved 389 animals. The sequencing results revealed the presence of 5 exons (10 bp, 161 bp, 117 bp, 162 bp, 201 bp) and 4 introns (245 bp, 171 bp, 176 bp, 290 bp), with a total length of 1745 bp. Fourteen polymorphic variable sites were identified: 12 SNP substitutions, one single nucleotide deletion and one ins/del polymorphism. The assessment of informativeness and conclusiveness of the identified variable sitesshows that the highest ability to...
Bushra Niaz1,*, Tahir Zahoor1, Muhammad Atif Randhawa1 and Amer Jamil2
...te in milk secretions of animals and humandisplays a variety of antimicrobial, antioxidant and immunomodulatory as well as a number of other biological functions. Its potential can be exploited in several food applications. Keeping in view the increased demand of natural antimicrobial agents to control prevalence of food borne pathogens in final packaged food products as well as for food preservation/ biocontrol, this study was designed to isolate lactoferrin ...
Sadia Munir1a, Asif Nadeem1a*, Maryam Javed1, Masroor Ellahi Babar2, Tanveer Hussain2, Wasim Shehzad1, Rajput Zahid Iqbal3 and Sidra Manzoor1
...he hormone expression in animals. In current study, Pit1 gene was screened for polymorphic sites in Sahiwal cattle. Samples from Sahiwal cattle breeds were sequenced using six set of primers. A total 15 polymorphisms; 12 in intronic and 3 in exonic region, were identified. The sequences of the amplified Pit1 gene fragments were aligned with the help of BLAST for SNPs identification. This is a first report toward genetic screening of this gene at ...
Burhan Azam1, Muhammad Naeem Tahir2,*, Faisal Shahzad2, Abdul Ghaffar3, Ghulam Abbas4, Madiha Gohar5 and Saima1
...0, T3 = 15 and T4 = 20 g/animal/day) in a 4 × 4 Latin Square change-over design with four experimental periods. Each period consisted of a 14-days enzyme supplementation period following a 3-day transition period without enzyme supplementation between consecutive experimental periods. The results showed a significant linear (P<0.001) increase in the intakes of DM, CP and NDF by the animals with increasing level of e...
Naimat Ullah Khan1,2, Muhammad Hassan Saleem1, Aneela Zameer Durrani1, Nisar Ahmad3, Ayesha Hassan1, Sultan Ayaz2, Sajid Umar4, Muhammad Luqman Sohail5,*, Muhammad Shafee6, Ikramullah Khan7, Mumtaz Ali Khan1, Azmat Ullah Khan8, Naimat Ullah Malik1 and Abdul Razzaq9
...ubjects having livestock animal contact and persistent diarrhea, highlighting its zoonotic potential from livestock animals to human beings in the study area. This is first study addressing the one of the main cause of neonatal mortality in Southern KPK and its zoonotic potential.
...
Wenqiao Hui1, Jishun Tang1, Dejian Zhu1, Qian Ban2* and Sheng Chen1*
...hysical maturation of an animal with the capability of sexual reproduction. In domestic animals, age at attainment of puberty is an event that contributes significantly to lifetime reproductive efficiency. Anhui white goat is a native Chinese breed, with the characters of early time puberty onset and high fecundity. However, the molecular mechanism of puberty onset has not been well understood. It has been demonstrated lepti...
Sidra Manzoor1,Asif Nadeem1,*, Masroor Ellahi Babar2, Wasim Shehzad1, Abu Saeed Hashmi1, Muhammad Imran1, Tanveer Hussain2, Abdul Wajid1 and Maryam Javed1
...ection efficacy of dairy animals, the effects of candidate polymorphism should further be screened in different environments and genetic backgrounds.
...
Furukh Faiz1,2, Muhammad Issa Khan2, Masood Sadiq2 and Haq Nawaz3
...effects on the health of animals and poultry. In the current studies the citrus waste is dried and mixed with the broiler feed replacing maize from the feed at 0%, 2.5%, 5%, 7.5% and 10%. 225 birds were reared in 5 treatments and 3 replications of each treatment having 15 birds in each replication. The chicks were being provided with the standard conditions for light, water, vaccination and experimental feed for 42 days. The body weight gain, feed intake and t...
Muhammad Imran Shabbir
... the basis for plant and animal breeding to get desired varieties as well as performing genetic analysis including prediction of patterns of inheritance in family lines and to calculate the recurrence risk for relatives etc. Punnett square is used to describe the possible combinations of paternal and maternal alleles for a particular cross. Described here is a line-dot method using ternary genotype codes to determine genotype probabilities in a very quick and ...
A. Hamid1,*, M. Ilyas2 and S. Kalsoom3
... the TPC in experimental animals compared with purified diet AIN-93, WBF and MBF. The VLDL, LDL and triglycerides levels were also decreased accordingly while a higher ratio of HDL was recorded in rats fed on BGF and SGF compared to the other feeds. The results suggest that BGF and SGF containing the soluble ß-glucan extract can reduce TPC, VLDL, LDL, and triglycerides and higher HDL.
...
Shuangye Wang1, Yunlin Zhao1, Zhenggang Xu1,2,*, Li Li3, Liang Wu1, Choucang Duan1 and Jiao Peng1
...t, it was found that the animal’s behavioural rhythms changed during acclimation to the new habitat. It is likely that resting and feeding behaviours were influenced by temperature.
...
Yijin He1, Song Ma2, Bo Liu1,*, Ting Xue1, Qunlan Zhou1, Wu Jin1 and Kui Chen2,*
...ne of human genome model animal since mid-1990s. In this experiment, we cloned a pair of DNA fragment sequence including complete ORF (921bp) of taste receptor candidate gene T2R1 of T. fasciatus and studied the differential expression spectrum of tissues in combination with bioinformatics analysis so as to understand the possible functions in response to chemical sensation behavior of taste receptors. The receptors when compared with T. rubri...
Rahat Naseer1*, Abu Saeed Hashmi1, Zulfiqar-ul-Hassan2, H. Rehman1, Saima Naveed1, F. Masood1 and M. Tayyab1
... offered to experimental animals (n=36, 22 male 14 female Lohi sheep almost evenly distributed in experimental groups) having almost uniform body weight (BW). During 60 days of the trial, no significant difference (p =0.067) in the daily feed intake among the different experimental groups was recorded. Maximum feed consumption was recorded in HT (hydrothermal treatment) 1096.69±26.8 (mean± SE) while lowest in NC (untreated husk) 974.91±18....
Rais Ahmed1,*, Khushi Muhammad1, Masood Rabbani1 and Muhammad Sarwar Khan2
...eproductive disorders in animals and human beings. In this project, prevalence and distribution of soil borne Brucella species in nine districts of Punjab including Lahore, Faisalabad, Sheikhupura, Sargodha, D.G. Khan, Chakwal, Sahiwal, Gujranwala and Attock were determined. By using grid based sampling strategy, soil samples (n= 1280) were collected from villages (n=256, 5 samples/village) of the nine districts. Brucella genus specific DNA (b...
Ahmed Zein Elabdeen Mahmoud1, Muaz Magzob Abdellatif2* and Mohamed Abdelsalam Abdalla3
... 648 (49.3%) of affected animals, the overall morbidity in goats (33.3%) was higher as compared to sheep (12.3%), with a case fatality rate in goats reached 74.69% in the 2nd outbreak. Affected animals exhibited fever, stomatitis, mucopurulent to bloody nasal/ocular discharges, watery to bloody diarrhea and lameness. The major gross lesions were erosive stomatitis, hemorrhages in lung, liver, abomasum, small and large intest...
Tiansen Li1, Meiling Huang2, Zhen Wang1,Fei Guo3,Hui Zhang1,* and Chuangfu Chen1,*
...enic bacteria that cause animal and human brucellosis. Currently, the mechanism behind the pathogenesis of Brucella remains unclear. For this reason omp10 mutant was constructed to examine the impacts of the outer membrane protein 10 (Omp10) on bacterial survival, virulence, phagosome-lysosome fusion, and apoptosis induction, as assessed in appropriate in vitro (cell culture) and animal models. Results s...
Kadir Karakuş1, Bahat Comba2, Abuzer Taş3, Tunahan Sancak3, Arzu Comba2, Devrim Saripinar Aksu4, Hasan Koyun5 and Mohammad Masood Tariq6,*
...om vena jugularis of the animals before the application and every two months for a total of 4 times for 6 months and hormone analyses were performed. The order of statistical significance for TSH (thyroid stimulating hormone) were found as Surgical > Control > Formol (P <0.05). The order of statistical significance for growth hormone was Formol = Surgery > Control (P<0.05). In August month, the application factor was found to be significant only...
Muhammad Sohail1, Muhammad Nauman-ul-Islam1, Hayazuddin2*, Imtiaz Ali Shah1, Abdur Raziq1, Subhan Ullah3 and Arsalan Ali Shah1
...us (n= 08). Experimental animals were allocated to diet containing zero (control), 12 and 18 percent MSC. All the three rations were iso-caloric and iso-nitrogeniuos. Each cow was given a daily feed ration according to NARC standards. Milk yield of each experimental animals were recorded twice daily while its composition were checked through milk analyzer twice a week. Mean daily production of milk among three groups was sig...
Shahid Hussain Farooqi1,*, Muhammad Ijaz1, Muhammad Hassan Saleem1, Muhammad Imran Rashid2, Muhammad Oneeb2, Amjad Khan3, Amjad Islam Aqib1 and Shakeel Mahmood4
...ected from four hundreds animals comprising of two hundred and fifty cattle and one hundred and fifty buffaloes. Descriptive statistics with Pearson’s Chi-square test and regression model were applied to analyze the data. The results of study found Rhepicephalus the most prevalent genus followed by Heamaphysalis, Hyalomma, Dermacenter and Amblyomma with the prevalence of 78.50%, 10.33%, 10.08%, 0.67% and 0.42%, respectively. On speci...
Kashif Prince1,*, Muhammad Sarwar Khan1, Muhammad Ijaz1, Aftab Ahmad Anjum2, Atif Prince3, Naimat Ullah Khan1 and Mumtaz Ali Khan1
...um with and without VES: animals in D and E were at day 60 prepartum with and without VES: animals in F and G were at 75 days prepartum stage with and without VES: respectively. At day 0 of experimental trial VES was given to animals in group C, E and G. All groups were given oil based HS vaccine at day 15 and alum based FMD trivalent vaccine at day 30 of experimental trial except Group A....
Habibun Nabi1, Saher Islam2, Amna Arshad Bajwa2, Imran Rashid1,*, Haroon Akbar1, Wasim Shehzad2, Kamran Ashraf1, Nisar Ahmad1 and Aneela Durrani3
...of T. gondii from animals and man need to be performed in Pakistan.
... 
Sanjukta Chakrabarti1, Colin J. Barrow2, Rupinder K. Kanwar3, Venkata Ramana1*, Rakesh N. Veedu4,5 and Jagat R. Kanwar3*
Nosheen Rafiq1, Asmatullah Kakar2,*, Arshad Ghani3, Asim Iqbal2, Wali Mohammad Achakzai2, Shagufta Sadozai2, Mohammad Shafiq4 and Mohammad Alam Mengal5
...ick counting with age of animals revealed positive and significant correlation (P = 0.1145, P < 0.05). The ratio of tick infestation was observed to be insignificant (P < 0.01) among the breeds, with most elevated prepotency in local (88.97%) and cross breeds (13.33%), respectively.
... 
